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2024-03-29T06:56:45Z
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http://2011.igem.org/Team:Fatih_Turkey/Results
Team:Fatih Turkey/Results
2011-09-22T04:07:13Z
<p>Metugen: </p>
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<a href="https://2011.igem.org/Team:Fatih_Turkey/Project">Project<span>Rainbow Graveyard</span></a><br />
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<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Project">Overall Project</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/LALF">LALF</a></li><br />
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<h2 style="font-family: Verdana, Arial, SunSans-Regular, sans-serif;">Project</h2><br />
<h5 style="font-family: Verdana, Arial, SunSans-Regular, sans-serif;">Results</h5> <br />
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<p><em>1. </em><em>technique;</em> in case of applying on surfaces, LALF protein can work like a new sterilization material like gluteraldehyte or alcohol that are commonly used for hospital and lab sterilization.<em> </em></p><br />
<p><em>2. </em><em>Alternative cure for septic shock;</em> after gram negative bacterial infections; LPS, which is the main component of cell wall of gram negative bacteria, can cause deathly conditions that is generally called “septic shock”. In our project, our LALF protein binds this material strictly, thus LALF can be used as an alternative cure method for such condition.<em> </em></p><br />
<p><em>3. </em><em>Possible new structure of antibiotic-like drug; </em>antibiotics commonly effect on production of cell wall of bacteria. This is done by inhibiting the source of production. On the other hand, our LALF protein directly binds the cell wall material and stops the bacteria growth. Thus, a new antibiotic like drug made of LALF may be produced and used in some infections.<em> </em></p><br />
<p><em>4. </em><em>Possibility of producing longtime sterile layers; </em>if any kind of object is produced by covering with LALF, that object can stay sterile for long time because of gram negative bacteria protection of LALF. In case of a possible infection on surface, LALF will stop bacteria growth.<em> </em></p><br />
<p><em>5. </em><em>Alternative cure method for bacterial diseases; </em>diseases like urinary system infection or gastritis are caused by gram negative bacteria.<em> </em>In the course of applying our bacteria that produce LALF in the infectious cite, infection of harmful bacteria can be prevented or stopped. This may suggest an alternative cure for such diseases.<em> </em></p><br />
<p>On the other hand, our secondary protein, reflectin, can be used in varying applications.</p><br />
<p><em>1. </em><em>Camouflage material; </em>this protein allows its host, cephalopod, to hide when cephalopod hunts or to defend itself from its hunters. In the course of development of reflectin protein, a new camouflage material may be produced for humans.<em> </em></p><br />
<p><em>2. </em><em>New material of dye for varying applications; </em>Dye that changes its color can be used almost every colorful application. From fireworks to wall paints, from windows of buildings to road signs, this ability can be very useful.<em></em></p><br />
<p><em>3. </em><em>Possibility of producing specialized light colors; </em>in some circumstances, a unique light color may be necessary in order to gain maximum output like in greenhouses or dark rooms in photography industry. Reflectin reflects the light by changing its wavelength; therefore the light that is reflected has only one color. This ability can be applied in such application that mentioned above.<em></em></p><br />
<p><strong><br /><br />
</strong></p><br />
<br />
<p>COLLABORATION </p><br />
<br />
<p>With friendly communications teams can take some useful advises and learn some <br />
<br />
important points. Also with helping to other groups friendly feelings increase <br />
<br />
and the team’s motivation increases. During our lab working we were always <br />
<br />
keeping touch with other teams. We were helping each other and making some <br />
<br />
activities together. </p><br />
<br />
<br />
<p>We helped to Bilkent_UNAM_Turkey team when they needed our part 1001 and they <br />
<br />
helped to us with giving LB Agar. Also the Team Michigan asked helping to us <br />
<br />
about board game. We designed the Canvas Town Game and the Team Michigan wanted <br />
<br />
some points about preparing and designing. And we were being glad to help them.<br />
Association of Synthetic Biology – METU-Ankara Team invited our team to <br />
<br />
Synthetic Biology Turkey Meetings 1.0 of Igem Turkey Teams. This meeting was <br />
<br />
really funny and informative. </p><br />
<br />
<br />
<p>We are planning a little tournament of Canvas Town Game in the Asia Religion <br />
<br />
Jamboree. For this organization we help from Religion Asia Organization <br />
<br />
Committee and they helped us very quickly and politely.</p><br />
<br />
<br />
<p>For the tournament four teams will determine with drawing. 2 teams will play <br />
<br />
together and the other 2 teams will play together. Just 6 persons can play our <br />
<br />
game so every team should play with their 3 members. Then the winner teams will <br />
<br />
play together in the end of this game 3 finalist member of the last winner team <br />
<br />
will be presented.</p><br />
<br />
<br />
<p>With these activities we were really enjoyed and informed.</p><br />
<br />
<br />
<br />
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<br />
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<div class="meta-left-full"> <br />
<tr style="height: 20px;vertical-align: top;"><th colspan="2" style="text-align: center; font-size: 20px; font-weight: bold;">RESULTS For our project lab executions;</th></tr><br />
<br />
<p> 1. LALF based anti-LPS factor system has been worked expectedly<em> </em></p><br />
<p><em>2.we have constructed and submitted 12 biobrick. Three devices working in two for <br />
<br />
B.subtilis one for E.coli work expectedly.<br />
<em> </em></p><br />
<p><em>3. Our B.subtilis specific promoter with SacB- extracellur signal- has been seen <br />
<br />
working well.<em> </em></p><br />
<br />
<p><em>4. We have synthesized Reflectin 1a protein in E.coli; however, because we did not <br />
<br />
have enough time to purify it to visualize via aid-eye, we could not use <br />
<br />
Reflectin 1a device as biosensor for our lalf-LPS interaction in B.subtilis-<br />
<br />
E.coli mixed culture. <em> </em></p><br />
<br />
<p>5. There are b.subtilis with LALF in our B1 plate and B.subtilis without LALF in our B2 plate.For working of our LALF protein ,count of e.coli with RFP in B1 is lower than B2.</p><br />
<br />
<br />
<p>6. We spread fresh E.coli which produce RFP and put them onto 100uL Bacillus Subtilis supernatant which include LALF (plate D1) and no LALF protein producing Bacillus Subtilis supernatant(plateD2).As you can see where Bacillus supernatant with LALF exist ,there wasn’t any E.coli with RFP.</p><br />
<br />
<br />
<p>7. We spread fresh E.coli which produce RFP and put them onto 100uL Bacillus Subtilis which include LALF (plateC1 ) and no LALF protein producing Bacillus Subtilis (plateC2).As you can see where Bacillus with LALF exist ,there wasn’t any E.coli with RFP.</p><br />
<br />
<p>8. We saw that size of colonies with LALF protein is smaller than size of colonies without LALF protein and also antibiotic didn’t changed the results.</p><br />
<br />
<p>9. IPTG was caused of suicide of E.coli.Our promoter is IPTG inducable.So when we added IPTG in the media LALF synthezised.Result of this experiment we can say that E.coli kills itself.</p><br />
<br />
<p>10. LALF find in the DNA sequence and mRNA sequence. We produced LALF protein by E. coli.</p><br />
<br />
<p>11. In conclusion, BBa_K541915, BBa_K541545, BBa_K541515, BBa_K541596, BBa_K541800, BBa_K541501, BBa_K541502, BBa_K541503, BBa_K541504, BBa_K541505, BBa_K541506, BBa_K541516, BBa_K541526, BBa_K541546 are confirmed. </p><br />
<br />
<p>12. LALF find in the DNA sequence and mRNA sequence. We produced LALF protein by E. coli.</p><br />
<br />
<p>13. Producing of expected part’s mRNA is confirmed by electrophoresis and sequence analysis.<br />
As a result, mRNA of LALF protein has been produced, the promoter is producing both LALF and GFP sequences.</p><br />
<br />
<p>14. Producing of expected part’s mRNA is confirmed by electrophoresis and sequence analysis.<br />
As a result, mRNA of LALF protein has been produced, the promoter is producing both LALF and GFP sequences.</p><br />
<br />
<p>15. After adding the FR solutions on all of plates, we measured their OD values in the end of this experiment we measured again and saw that the 1st, 2nd, 3rd, 4th plates OD values didn’t change. But the the OD value of 5th plate increased. 5th plate is the control group. OFR is more effective than DFR for B,subtilis.<br />
<br />
DFR is more effective than OFR for E.coli.</p><br />
<br />
<br />
<br />
<tr style="height: 20px;vertical-align: top;"><th colspan="2" style="text-align: center; font-size: 20px; font-weight: bold;"><strong>For Human Practice; We have performed the activities as follows;</strong></th></tr><br />
<br />
<p><strong><em></em><em></em></strong></p><br />
<br />
<p>1. Providing Sporocide Method for prevention from spores of B.subtilis in further <br />
<br />
B.subtilis synthetic biology project.<em> </em></p><br />
<br />
<p>2. Preparing a novel board game `Canvas Time` to inform people from any distinct <br />
<br />
education level about features of our project, laboratory equipments and <br />
<br />
interesting Biobrick of iGEM competition.<br />
.</p><br />
<p>3.Performing open-lab to public meetings such as gifted children studying in <br />
<br />
several schools of Ankara, undergraduate students from Hacettepe University <br />
<br />
Medical School and postgraduate students from Ankara University Medical School<br />
</p><br />
<p>4.Kindergarten synthetic biology trip to teach synthetic biology to five-year-old <br />
<br />
children. <br />
</p><br />
<p><strong><br /><br />
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Team:Fatih Turkey/Results
2011-09-22T04:02:36Z
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<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Sporocide">Sporocide</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/iGEM_for_7_to_77">iGEM for 7 to 77</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/game">Game</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/canvas_times">Canvas Times</a></li><br />
</ul><br />
</li><br />
<li><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey/Lab_Garage">Lab Garage</a><br />
<ul><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Notebook">Notebook</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Procedures">Procedures</a></li><br />
</ul><br />
</li><br />
</ul><br />
</div><br />
</div><br />
</div><br />
</div> <br />
</div><br />
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<div class="bar-title-whole"><br />
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<h2 style="font-family: Verdana, Arial, SunSans-Regular, sans-serif;">Project</h2><br />
<h5 style="font-family: Verdana, Arial, SunSans-Regular, sans-serif;">Results</h5> <br />
</div> <br />
<div class="bar-title-sh-left"></div><br />
<div class="bar-title-sh"></div><br />
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<p><em>1. </em><em>technique;</em> in case of applying on surfaces, LALF protein can work like a new sterilization material like gluteraldehyte or alcohol that are commonly used for hospital and lab sterilization.<em> </em></p><br />
<p><em>2. </em><em>Alternative cure for septic shock;</em> after gram negative bacterial infections; LPS, which is the main component of cell wall of gram negative bacteria, can cause deathly conditions that is generally called “septic shock”. In our project, our LALF protein binds this material strictly, thus LALF can be used as an alternative cure method for such condition.<em> </em></p><br />
<p><em>3. </em><em>Possible new structure of antibiotic-like drug; </em>antibiotics commonly effect on production of cell wall of bacteria. This is done by inhibiting the source of production. On the other hand, our LALF protein directly binds the cell wall material and stops the bacteria growth. Thus, a new antibiotic like drug made of LALF may be produced and used in some infections.<em> </em></p><br />
<p><em>4. </em><em>Possibility of producing longtime sterile layers; </em>if any kind of object is produced by covering with LALF, that object can stay sterile for long time because of gram negative bacteria protection of LALF. In case of a possible infection on surface, LALF will stop bacteria growth.<em> </em></p><br />
<p><em>5. </em><em>Alternative cure method for bacterial diseases; </em>diseases like urinary system infection or gastritis are caused by gram negative bacteria.<em> </em>In the course of applying our bacteria that produce LALF in the infectious cite, infection of harmful bacteria can be prevented or stopped. This may suggest an alternative cure for such diseases.<em> </em></p><br />
<p>On the other hand, our secondary protein, reflectin, can be used in varying applications.</p><br />
<p><em>1. </em><em>Camouflage material; </em>this protein allows its host, cephalopod, to hide when cephalopod hunts or to defend itself from its hunters. In the course of development of reflectin protein, a new camouflage material may be produced for humans.<em> </em></p><br />
<p><em>2. </em><em>New material of dye for varying applications; </em>Dye that changes its color can be used almost every colorful application. From fireworks to wall paints, from windows of buildings to road signs, this ability can be very useful.<em></em></p><br />
<p><em>3. </em><em>Possibility of producing specialized light colors; </em>in some circumstances, a unique light color may be necessary in order to gain maximum output like in greenhouses or dark rooms in photography industry. Reflectin reflects the light by changing its wavelength; therefore the light that is reflected has only one color. This ability can be applied in such application that mentioned above.<em></em></p><br />
<p><strong><br /><br />
</strong></p><br />
<br />
<p>COLLABORATION </p><br />
<br />
<p>With friendly communications teams can take some useful advises and learn some <br />
<br />
important points. Also with helping to other groups friendly feelings increase <br />
<br />
and the team’s motivation increases. During our lab working we were always <br />
<br />
keeping touch with other teams. We were helping each other and making some <br />
<br />
activities together. </p><br />
<br />
<br />
<p>We helped to Bilkent_UNAM_Turkey team when they needed our part 1001 and they <br />
<br />
helped to us with giving LB Agar. Also the Team Michigan asked helping to us <br />
<br />
about board game. We designed the Canvas Town Game and the Team Michigan wanted <br />
<br />
some points about preparing and designing. And we were being glad to help them.<br />
Association of Synthetic Biology – METU-Ankara Team invited our team to <br />
<br />
Synthetic Biology Turkey Meetings 1.0 of Igem Turkey Teams. This meeting was <br />
<br />
really funny and informative. </p><br />
<br />
<br />
<p>We are planning a little tournament of Canvas Town Game in the Asia Religion <br />
<br />
Jamboree. For this organization we help from Religion Asia Organization <br />
<br />
Committee and they helped us very quickly and politely.</p><br />
<br />
<br />
<p>For the tournament four teams will determine with drawing. 2 teams will play <br />
<br />
together and the other 2 teams will play together. Just 6 persons can play our <br />
<br />
game so every team should play with their 3 members. Then the winner teams will <br />
<br />
play together in the end of this game 3 finalist member of the last winner team <br />
<br />
will be presented.</p><br />
<br />
<br />
<p>With these activities we were really enjoyed and informed.</p><br />
<br />
<br />
<br />
<p><div><br />
<br />
<div><br />
<div class="meta-left-full"> <br />
<tr style="height: 20px;vertical-align: top;"><th colspan="2" style="text-align: center; font-size: 20px; font-weight: bold;">RESULTS For our project lab executions;</th></tr><br />
<br />
<p> 1. LALF based anti-LPS factor system has been worked expectedly<em> </em></p><br />
<p><em>2.we have constructed and submitted 12 biobrick. Three devices working in two for <br />
<br />
B.subtilis one for E.coli work expectedly.<br />
<em> </em></p><br />
<p><em>3. Our B.subtilis specific promoter with SacB- extracellur signal- has been seen <br />
<br />
working well.<em> </em></p><br />
<br />
<p><em>4. We have synthesized Reflectin 1a protein in E.coli; however, because we did not <br />
<br />
have enough time to purify it to visualize via aid-eye, we could not use <br />
<br />
Reflectin 1a device as biosensor for our lalf-LPS interaction in B.subtilis-<br />
<br />
E.coli mixed culture. <em> </em></p><br />
<br />
<p>5. There are b.subtilis with LALF in our B1 plate and B.subtilis without LALF in our B2 plate.For working of our LALF protein ,count of e.coli with RFP in B1 is lower than B2.</p><br />
<br />
<br />
<p>6. We spread fresh E.coli which produce RFP and put them onto 100uL Bacillus Subtilis supernatant which include LALF (plate D1) and no LALF protein producing Bacillus Subtilis supernatant(plateD2).As you can see where Bacillus supernatant with LALF exist ,there wasn’t any E.coli with RFP.</p><br />
<br />
<br />
<p>7. We spread fresh E.coli which produce RFP and put them onto 100uL Bacillus Subtilis which include LALF (plateC1 ) and no LALF protein producing Bacillus Subtilis (plateC2).As you can see where Bacillus with LALF exist ,there wasn’t any E.coli with RFP.</p><br />
<br />
<p>8. We saw that size of colonies with LALF protein is smaller than size of colonies without LALF protein and also antibiotic didn’t changed the results.</p><br />
<br />
<p>9. IPTG was caused of suicide of E.coli.Our promoter is IPTG inducable.So when we added IPTG in the media LALF synthezised.Result of this experiment we can say that E.coli kills itself.</p><br />
<br />
<p>10. LALF find in the DNA sequence and mRNA sequence. We produced LALF protein by E. coli.</p><br />
<br />
<p>11. In conclusion, BBa_K541915, BBa_K541545, BBa_K541515, BBa_K541596, BBa_K541800, BBa_K541501, BBa_K541502, BBa_K541503, BBa_K541504, BBa_K541505, BBa_K541506, BBa_K541516, BBa_K541526, BBa_K541546 are confirmed. </p><br />
<br />
<p>12. LALF find in the DNA sequence and mRNA sequence. We produced LALF protein by E. coli.</p><br />
<br />
<p>13. Producing of expected part’s mRNA is confirmed by electrophoresis and sequence analysis.<br />
As a result, mRNA of LALF protein has been produced, the promoter is producing both LALF and GFP sequences.</p><br />
<br />
<p>14. Producing of expected part’s mRNA is confirmed by electrophoresis and sequence analysis.<br />
As a result, mRNA of LALF protein has been produced, the promoter is producing both LALF and GFP sequences.</p><br />
<br />
<p>15. After adding the FR solutions on all of plates, we measured their OD values in the end of this experiment we measured again and saw that the 1st, 2nd, 3rd, 4th plates OD values didn’t change. But the the OD value of 5th plate increased. 5th plate is the control group. OFR is more effective than DFR for B,subtilis.<br />
<br />
DFR is more effective than OFR for E.coli.</p><br />
<br />
<br />
<br />
<tr style="height: 20px;vertical-align: top;"><th colspan="2" style="text-align: center; font-size: 20px; font-weight: bold;"><strong>For Human Practice; We have performed the activities as follows;</strong></th></tr><br />
<br />
<p><strong><em></em><em></em></strong></p><br />
<br />
<p>1. Providing Sporocide Method for prevention from spores of B.subtilis in further <br />
<br />
B.subtilis synthetic biology project.<em> </em></p><br />
<br />
<p>2. Preparing a novel board game `Canvas Time` to inform people from any distinct <br />
<br />
education level about features of our project, laboratory equipments and <br />
<br />
interesting Biobrick of iGEM competition.<br />
.</p><br />
<p>3.Performing open-lab to public meetings such as gifted children studying in <br />
<br />
several schools of Ankara, undergraduate students from Hacettepe University <br />
<br />
Medical School and postgraduate students from Ankara University Medical School<br />
</p><br />
<p>4.Kindergarten synthetic biology trip to teach synthetic biology to five-year-old <br />
<br />
children. <br />
</p><br />
<p><strong><br /><br />
</strong></p><br />
<br />
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Metugen
http://2011.igem.org/Team:Fatih_Turkey/Results
Team:Fatih Turkey/Results
2011-09-22T03:59:21Z
<p>Metugen: </p>
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<h2 style="font-family: Verdana, Arial, SunSans-Regular, sans-serif;">Project</h2><br />
<h5 style="font-family: Verdana, Arial, SunSans-Regular, sans-serif;">Results</h5> <br />
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<p><em>1. </em><em>technique;</em> in case of applying on surfaces, LALF protein can work like a new sterilization material like gluteraldehyte or alcohol that are commonly used for hospital and lab sterilization.<em> </em></p><br />
<p><em>2. </em><em>Alternative cure for septic shock;</em> after gram negative bacterial infections; LPS, which is the main component of cell wall of gram negative bacteria, can cause deathly conditions that is generally called “septic shock”. In our project, our LALF protein binds this material strictly, thus LALF can be used as an alternative cure method for such condition.<em> </em></p><br />
<p><em>3. </em><em>Possible new structure of antibiotic-like drug; </em>antibiotics commonly effect on production of cell wall of bacteria. This is done by inhibiting the source of production. On the other hand, our LALF protein directly binds the cell wall material and stops the bacteria growth. Thus, a new antibiotic like drug made of LALF may be produced and used in some infections.<em> </em></p><br />
<p><em>4. </em><em>Possibility of producing longtime sterile layers; </em>if any kind of object is produced by covering with LALF, that object can stay sterile for long time because of gram negative bacteria protection of LALF. In case of a possible infection on surface, LALF will stop bacteria growth.<em> </em></p><br />
<p><em>5. </em><em>Alternative cure method for bacterial diseases; </em>diseases like urinary system infection or gastritis are caused by gram negative bacteria.<em> </em>In the course of applying our bacteria that produce LALF in the infectious cite, infection of harmful bacteria can be prevented or stopped. This may suggest an alternative cure for such diseases.<em> </em></p><br />
<p>On the other hand, our secondary protein, reflectin, can be used in varying applications.</p><br />
<p><em>1. </em><em>Camouflage material; </em>this protein allows its host, cephalopod, to hide when cephalopod hunts or to defend itself from its hunters. In the course of development of reflectin protein, a new camouflage material may be produced for humans.<em> </em></p><br />
<p><em>2. </em><em>New material of dye for varying applications; </em>Dye that changes its color can be used almost every colorful application. From fireworks to wall paints, from windows of buildings to road signs, this ability can be very useful.<em></em></p><br />
<p><em>3. </em><em>Possibility of producing specialized light colors; </em>in some circumstances, a unique light color may be necessary in order to gain maximum output like in greenhouses or dark rooms in photography industry. Reflectin reflects the light by changing its wavelength; therefore the light that is reflected has only one color. This ability can be applied in such application that mentioned above.<em></em></p><br />
<p><strong><br /><br />
</strong></p><br />
<br />
<p>COLLABORATION </p><br />
<br />
<p>With friendly communications teams can take some useful advises and learn some <br />
<br />
important points. Also with helping to other groups friendly feelings increase <br />
<br />
and the team’s motivation increases. During our lab working we were always <br />
<br />
keeping touch with other teams. We were helping each other and making some <br />
<br />
activities together. </p><br />
<br />
<br />
<p>We helped to Bilkent_UNAM_Turkey team when they needed our part 1001 and they <br />
<br />
helped to us with giving LB Agar. Also the Team Michigan asked helping to us <br />
<br />
about board game. We designed the Canvas Town Game and the Team Michigan wanted <br />
<br />
some points about preparing and designing. And we were being glad to help them.<br />
Association of Synthetic Biology – METU-Ankara Team invited our team to <br />
<br />
Synthetic Biology Turkey Meetings 1.0 of Igem Turkey Teams. This meeting was <br />
<br />
really funny and informative. </p><br />
<br />
<br />
<p>We are planning a little tournament of Canvas Town Game in the Asia Religion <br />
<br />
Jamboree. For this organization we help from Religion Asia Organization <br />
<br />
Committee and they helped us very quickly and politely.</p><br />
<br />
<br />
<p>For the tournament four teams will determine with drawing. 2 teams will play <br />
<br />
together and the other 2 teams will play together. Just 6 persons can play our <br />
<br />
game so every team should play with their 3 members. Then the winner teams will <br />
<br />
play together in the end of this game 3 finalist member of the last winner team <br />
<br />
will be presented.</p><br />
<br />
<br />
<p>With these activities we were really enjoyed and informed.</p><br />
<br />
<br />
<br />
<p><div><br />
<br />
<div><br />
<div class="meta-left-full"> <br />
<tr style="height: 20px;vertical-align: top;"><th colspan="2" style="text-align: center; font-size: 20px; font-weight: bold;">RESULTS For our project lab executions;</th></tr><br />
<br />
<p> 1. LALF based anti-LPS factor system has been worked expectedly<em> </em></p><br />
<p><em>2.we have constructed and submitted 12 biobrick. Three devices working in two for <br />
<br />
B.subtilis one for E.coli work expectedly.<br />
<em> </em></p><br />
<p><em>3. Our B.subtilis specific promoter with SacB- extracellur signal- has been seen <br />
<br />
working well.<em> </em></p><br />
<br />
<p><em>4. We have synthesized Reflectin 1a protein in E.coli; however, because we did not <br />
<br />
have enough time to purify it to visualize via aid-eye, we could not use <br />
<br />
Reflectin 1a device as biosensor for our lalf-LPS interaction in B.subtilis-<br />
<br />
E.coli mixed culture. <em> </em></p><br />
<br />
<p>5. There are b.subtilis with LALF in our B1 plate and B.subtilis without LALF in our B2 plate.For working of our LALF protein ,count of e.coli with RFP in B1 is lower than B2.</p><br />
<br />
<br />
<p>6. We spread fresh E.coli which produce RFP and put them onto 100uL Bacillus Subtilis supernatant which include LALF (plate D1) and no LALF protein producing Bacillus Subtilis supernatant(plateD2).As you can see where Bacillus supernatant with LALF exist ,there wasn’t any E.coli with RFP.</p><br />
<br />
<br />
<p>7. We spread fresh E.coli which produce RFP and put them onto 100uL Bacillus Subtilis which include LALF (plateC1 ) and no LALF protein producing Bacillus Subtilis (plateC2).As you can see where Bacillus with LALF exist ,there wasn’t any E.coli with RFP.</p><br />
<br />
<p>8. We saw that size of colonies with LALF protein is smaller than size of colonies without LALF protein and also antibiotic didn’t changed the results.</p><br />
<br />
<p>9. IPTG was caused of suicide of E.coli.Our promoter is IPTG inducable.So when we added IPTG in the media LALF synthezised.Result of this experiment we can say that E.coli kills itself.</p><br />
<br />
<p>10. LALF find in the DNA sequence and mRNA sequence. We produced LALF protein by E. coli.</p><br />
<br />
<p>11. In conclusion, BBa_K541915, BBa_K541545, BBa_K541515, BBa_K541596, BBa_K541800, BBa_K541501, BBa_K541502, BBa_K541503, BBa_K541504, BBa_K541505, BBa_K541506, BBa_K541516, BBa_K541526, BBa_K541546 are confirmed. </p><br />
<br />
<p>12. LALF find in the DNA sequence and mRNA sequence. We produced LALF protein by E. coli.</p><br />
<br />
<p>13. Producing of expected part’s mRNA is confirmed by electrophoresis and sequence analysis.<br />
As a result, mRNA of LALF protein has been produced, the promoter is producing both LALF and GFP sequences.</p><br />
<br />
<p>14. Producing of expected part’s mRNA is confirmed by electrophoresis and sequence analysis.<br />
As a result, mRNA of LALF protein has been produced, the promoter is producing both LALF and GFP sequences.</p><br />
<br />
<p>15. After adding the FR solutions on all of plates, we measured their OD values in the end of this experiment we measured again and saw that the 1st, 2nd, 3rd, 4th plates OD values didn’t change. But the the OD value of 5th plate increased. 5th plate is the control group. OFR is more effective than DFR for B,subtilis.<br />
<br />
DFR is more effective than OFR for E.coli.</p><br />
<br />
<br />
<br />
<br />
<br />
<br />
<p><strong><em></em><em>For Human Practice; We have performed the activities as follows;</em></strong></p><br />
<br />
<p>1. Providing Sporocide Method for prevention from spores of B.subtilis in further <br />
<br />
B.subtilis synthetic biology project.<em> </em></p><br />
<br />
<p>2. Preparing a novel board game `Canvas Time` to inform people from any distinct <br />
<br />
education level about features of our project, laboratory equipments and <br />
<br />
interesting Biobrick of iGEM competition.<br />
.</p><br />
<p>3.Performing open-lab to public meetings such as gifted children studying in <br />
<br />
several schools of Ankara, undergraduate students from Hacettepe University <br />
<br />
Medical School and postgraduate students from Ankara University Medical School<br />
</p><br />
<p>4.Kindergarten synthetic biology trip to teach synthetic biology to five-year-old <br />
<br />
children. <br />
</p><br />
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http://2011.igem.org/Team:Fatih_Turkey/Results
Team:Fatih Turkey/Results
2011-09-22T03:57:43Z
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<h2 style="font-family: Verdana, Arial, SunSans-Regular, sans-serif;">Project</h2><br />
<h5 style="font-family: Verdana, Arial, SunSans-Regular, sans-serif;">Results</h5> <br />
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<p><em>1. </em><em>technique;</em> in case of applying on surfaces, LALF protein can work like a new sterilization material like gluteraldehyte or alcohol that are commonly used for hospital and lab sterilization.<em> </em></p><br />
<p><em>2. </em><em>Alternative cure for septic shock;</em> after gram negative bacterial infections; LPS, which is the main component of cell wall of gram negative bacteria, can cause deathly conditions that is generally called “septic shock”. In our project, our LALF protein binds this material strictly, thus LALF can be used as an alternative cure method for such condition.<em> </em></p><br />
<p><em>3. </em><em>Possible new structure of antibiotic-like drug; </em>antibiotics commonly effect on production of cell wall of bacteria. This is done by inhibiting the source of production. On the other hand, our LALF protein directly binds the cell wall material and stops the bacteria growth. Thus, a new antibiotic like drug made of LALF may be produced and used in some infections.<em> </em></p><br />
<p><em>4. </em><em>Possibility of producing longtime sterile layers; </em>if any kind of object is produced by covering with LALF, that object can stay sterile for long time because of gram negative bacteria protection of LALF. In case of a possible infection on surface, LALF will stop bacteria growth.<em> </em></p><br />
<p><em>5. </em><em>Alternative cure method for bacterial diseases; </em>diseases like urinary system infection or gastritis are caused by gram negative bacteria.<em> </em>In the course of applying our bacteria that produce LALF in the infectious cite, infection of harmful bacteria can be prevented or stopped. This may suggest an alternative cure for such diseases.<em> </em></p><br />
<p>On the other hand, our secondary protein, reflectin, can be used in varying applications.</p><br />
<p><em>1. </em><em>Camouflage material; </em>this protein allows its host, cephalopod, to hide when cephalopod hunts or to defend itself from its hunters. In the course of development of reflectin protein, a new camouflage material may be produced for humans.<em> </em></p><br />
<p><em>2. </em><em>New material of dye for varying applications; </em>Dye that changes its color can be used almost every colorful application. From fireworks to wall paints, from windows of buildings to road signs, this ability can be very useful.<em></em></p><br />
<p><em>3. </em><em>Possibility of producing specialized light colors; </em>in some circumstances, a unique light color may be necessary in order to gain maximum output like in greenhouses or dark rooms in photography industry. Reflectin reflects the light by changing its wavelength; therefore the light that is reflected has only one color. This ability can be applied in such application that mentioned above.<em></em></p><br />
<p><strong><br /><br />
</strong></p><br />
<br />
<p>COLLABORATION </p><br />
<br />
<p>With friendly communications teams can take some useful advises and learn some <br />
<br />
important points. Also with helping to other groups friendly feelings increase <br />
<br />
and the team’s motivation increases. During our lab working we were always <br />
<br />
keeping touch with other teams. We were helping each other and making some <br />
<br />
activities together. </p><br />
<br />
<br />
<p>We helped to Bilkent_UNAM_Turkey team when they needed our part 1001 and they <br />
<br />
helped to us with giving LB Agar. Also the Team Michigan asked helping to us <br />
<br />
about board game. We designed the Canvas Town Game and the Team Michigan wanted <br />
<br />
some points about preparing and designing. And we were being glad to help them.<br />
Association of Synthetic Biology – METU-Ankara Team invited our team to <br />
<br />
Synthetic Biology Turkey Meetings 1.0 of Igem Turkey Teams. This meeting was <br />
<br />
really funny and informative. </p><br />
<br />
<br />
<p>We are planning a little tournament of Canvas Town Game in the Asia Religion <br />
<br />
Jamboree. For this organization we help from Religion Asia Organization <br />
<br />
Committee and they helped us very quickly and politely.</p><br />
<br />
<br />
<p>For the tournament four teams will determine with drawing. 2 teams will play <br />
<br />
together and the other 2 teams will play together. Just 6 persons can play our <br />
<br />
game so every team should play with their 3 members. Then the winner teams will <br />
<br />
play together in the end of this game 3 finalist member of the last winner team <br />
<br />
will be presented.</p><br />
<br />
<br />
<p>With these activities we were really enjoyed and informed.</p><br />
<br />
<br />
<br />
<p><div><br />
<br />
<div><br />
<div class="meta-left-full"> <br />
<p><strong><em></em><em>For our project lab executions;</em></strong></p><br />
<br />
<p> 1. LALF based anti-LPS factor system has been worked expectedly<em> </em></p><br />
<p><em>2.we have constructed and submitted 12 biobrick. Three devices working in two for <br />
<br />
B.subtilis one for E.coli work expectedly.<br />
<em> </em></p><br />
<p><em>3. Our B.subtilis specific promoter with SacB- extracellur signal- has been seen <br />
<br />
working well.<em> </em></p><br />
<br />
<p><em>4. We have synthesized Reflectin 1a protein in E.coli; however, because we did not <br />
<br />
have enough time to purify it to visualize via aid-eye, we could not use <br />
<br />
Reflectin 1a device as biosensor for our lalf-LPS interaction in B.subtilis-<br />
<br />
E.coli mixed culture. <em> </em></p><br />
<br />
<p>5. There are b.subtilis with LALF in our B1 plate and B.subtilis without LALF in our B2 plate.For working of our LALF protein ,count of e.coli with RFP in B1 is lower than B2.</p><br />
<br />
<br />
<p>6. We spread fresh E.coli which produce RFP and put them onto 100uL Bacillus Subtilis supernatant which include LALF (plate D1) and no LALF protein producing Bacillus Subtilis supernatant(plateD2).As you can see where Bacillus supernatant with LALF exist ,there wasn’t any E.coli with RFP.</p><br />
<br />
<br />
<p>7. We spread fresh E.coli which produce RFP and put them onto 100uL Bacillus Subtilis which include LALF (plateC1 ) and no LALF protein producing Bacillus Subtilis (plateC2).As you can see where Bacillus with LALF exist ,there wasn’t any E.coli with RFP.</p><br />
<br />
<p>8. We saw that size of colonies with LALF protein is smaller than size of colonies without LALF protein and also antibiotic didn’t changed the results.</p><br />
<br />
<p>9. IPTG was caused of suicide of E.coli.Our promoter is IPTG inducable.So when we added IPTG in the media LALF synthezised.Result of this experiment we can say that E.coli kills itself.</p><br />
<br />
<p>10. LALF find in the DNA sequence and mRNA sequence. We produced LALF protein by E. coli.</p><br />
<br />
<p>11. In conclusion, BBa_K541915, BBa_K541545, BBa_K541515, BBa_K541596, BBa_K541800, BBa_K541501, BBa_K541502, BBa_K541503, BBa_K541504, BBa_K541505, BBa_K541506, BBa_K541516, BBa_K541526, BBa_K541546 are confirmed. </p><br />
<br />
<p>12. LALF find in the DNA sequence and mRNA sequence. We produced LALF protein by E. coli.</p><br />
<br />
<p>13. Producing of expected part’s mRNA is confirmed by electrophoresis and sequence analysis.<br />
As a result, mRNA of LALF protein has been produced, the promoter is producing both LALF and GFP sequences.</p><br />
<br />
<p>14. Producing of expected part’s mRNA is confirmed by electrophoresis and sequence analysis.<br />
As a result, mRNA of LALF protein has been produced, the promoter is producing both LALF and GFP sequences.</p><br />
<br />
<p>15. After adding the FR solutions on all of plates, we measured their OD values in the end of this experiment we measured again and saw that the 1st, 2nd, 3rd, 4th plates OD values didn’t change. But the the OD value of 5th plate increased. 5th plate is the control group. OFR is more effective than DFR for B,subtilis.<br />
<br />
DFR is more effective than OFR for E.coli.</p><br />
<br />
<br />
<br />
<br />
<br />
<br />
<p><strong><em></em><em>For Human Practice; We have performed the activities as follows;</em></strong></p><br />
<br />
<p>1. Providing Sporocide Method for prevention from spores of B.subtilis in further <br />
<br />
B.subtilis synthetic biology project.<em> </em></p><br />
<br />
<p>2. Preparing a novel board game `Canvas Time` to inform people from any distinct <br />
<br />
education level about features of our project, laboratory equipments and <br />
<br />
interesting Biobrick of iGEM competition.<br />
.</p><br />
<p>3.Performing open-lab to public meetings such as gifted children studying in <br />
<br />
several schools of Ankara, undergraduate students from Hacettepe University <br />
<br />
Medical School and postgraduate students from Ankara University Medical School<br />
</p><br />
<p>4.Kindergarten synthetic biology trip to teach synthetic biology to five-year-old <br />
<br />
children. <br />
</p><br />
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Metugen
http://2011.igem.org/Team:Fatih_Turkey/Results
Team:Fatih Turkey/Results
2011-09-22T03:57:14Z
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<a href="https://2011.igem.org/Team:Fatih_Turkey/Project">Project<span>Rainbow Graveyard</span></a><br />
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<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Project">Overall Project</a></li><br />
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<h2 style="font-family: Verdana, Arial, SunSans-Regular, sans-serif;">Project</h2><br />
<h5 style="font-family: Verdana, Arial, SunSans-Regular, sans-serif;">Results</h5> <br />
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<p><em>1. </em><em>technique;</em> in case of applying on surfaces, LALF protein can work like a new sterilization material like gluteraldehyte or alcohol that are commonly used for hospital and lab sterilization.<em> </em></p><br />
<p><em>2. </em><em>Alternative cure for septic shock;</em> after gram negative bacterial infections; LPS, which is the main component of cell wall of gram negative bacteria, can cause deathly conditions that is generally called “septic shock”. In our project, our LALF protein binds this material strictly, thus LALF can be used as an alternative cure method for such condition.<em> </em></p><br />
<p><em>3. </em><em>Possible new structure of antibiotic-like drug; </em>antibiotics commonly effect on production of cell wall of bacteria. This is done by inhibiting the source of production. On the other hand, our LALF protein directly binds the cell wall material and stops the bacteria growth. Thus, a new antibiotic like drug made of LALF may be produced and used in some infections.<em> </em></p><br />
<p><em>4. </em><em>Possibility of producing longtime sterile layers; </em>if any kind of object is produced by covering with LALF, that object can stay sterile for long time because of gram negative bacteria protection of LALF. In case of a possible infection on surface, LALF will stop bacteria growth.<em> </em></p><br />
<p><em>5. </em><em>Alternative cure method for bacterial diseases; </em>diseases like urinary system infection or gastritis are caused by gram negative bacteria.<em> </em>In the course of applying our bacteria that produce LALF in the infectious cite, infection of harmful bacteria can be prevented or stopped. This may suggest an alternative cure for such diseases.<em> </em></p><br />
<p>On the other hand, our secondary protein, reflectin, can be used in varying applications.</p><br />
<p><em>1. </em><em>Camouflage material; </em>this protein allows its host, cephalopod, to hide when cephalopod hunts or to defend itself from its hunters. In the course of development of reflectin protein, a new camouflage material may be produced for humans.<em> </em></p><br />
<p><em>2. </em><em>New material of dye for varying applications; </em>Dye that changes its color can be used almost every colorful application. From fireworks to wall paints, from windows of buildings to road signs, this ability can be very useful.<em></em></p><br />
<p><em>3. </em><em>Possibility of producing specialized light colors; </em>in some circumstances, a unique light color may be necessary in order to gain maximum output like in greenhouses or dark rooms in photography industry. Reflectin reflects the light by changing its wavelength; therefore the light that is reflected has only one color. This ability can be applied in such application that mentioned above.<em></em></p><br />
<p><strong><br /><br />
</strong></p><br />
<br />
<p>COLLABORATION </p><br />
<br />
<p>With friendly communications teams can take some useful advises and learn some <br />
<br />
important points. Also with helping to other groups friendly feelings increase <br />
<br />
and the team’s motivation increases. During our lab working we were always <br />
<br />
keeping touch with other teams. We were helping each other and making some <br />
<br />
activities together. </p><br />
<br />
<br />
<p>We helped to Bilkent_UNAM_Turkey team when they needed our part 1001 and they <br />
<br />
helped to us with giving LB Agar. Also the Team Michigan asked helping to us <br />
<br />
about board game. We designed the Canvas Town Game and the Team Michigan wanted <br />
<br />
some points about preparing and designing. And we were being glad to help them.<br />
Association of Synthetic Biology – METU-Ankara Team invited our team to <br />
<br />
Synthetic Biology Turkey Meetings 1.0 of Igem Turkey Teams. This meeting was <br />
<br />
really funny and informative. </p><br />
<br />
<br />
<p>We are planning a little tournament of Canvas Town Game in the Asia Religion <br />
<br />
Jamboree. For this organization we help from Religion Asia Organization <br />
<br />
Committee and they helped us very quickly and politely.</p><br />
<br />
<br />
<p>For the tournament four teams will determine with drawing. 2 teams will play <br />
<br />
together and the other 2 teams will play together. Just 6 persons can play our <br />
<br />
game so every team should play with their 3 members. Then the winner teams will <br />
<br />
play together in the end of this game 3 finalist member of the last winner team <br />
<br />
will be presented.</p><br />
<br />
<br />
<p>With these activities we were really enjoyed and informed.</p><br />
<br />
<br />
<br />
<p><div><br />
<br />
<div><br />
<div class="meta-left-full"> <br />
<p><strong><em></em><em>For our project lab executions;</em></strong></p><br />
<br />
<p> 1. LALF based anti-LPS factor system has been worked expectedly<em> </em></p><br />
<p><em>2.we have constructed and submitted 12 biobrick. Three devices working in two for <br />
<br />
B.subtilis one for E.coli work expectedly.<br />
<em> </em></p><br />
<p><em>3. Our B.subtilis specific promoter with SacB- extracellur signal- has been seen <br />
<br />
working well.<em> </em></p><br />
<br />
<p><em>4. We have synthesized Reflectin 1a protein in E.coli; however, because we did not <br />
<br />
have enough time to purify it to visualize via aid-eye, we could not use <br />
<br />
Reflectin 1a device as biosensor for our lalf-LPS interaction in B.subtilis-<br />
<br />
E.coli mixed culture. <em> </em></p><br />
<br />
<p>DISC EXPERIMENT1</p><br />
<br />
<p>5. There are b.subtilis with LALF in our B1 plate and B.subtilis without LALF in our B2 plate.For working of our LALF protein ,count of e.coli with RFP in B1 is lower than B2.</p><br />
<br />
<br />
<p>6. We spread fresh E.coli which produce RFP and put them onto 100uL Bacillus Subtilis supernatant which include LALF (plate D1) and no LALF protein producing Bacillus Subtilis supernatant(plateD2).As you can see where Bacillus supernatant with LALF exist ,there wasn’t any E.coli with RFP.</p><br />
<br />
<br />
<p>7. We spread fresh E.coli which produce RFP and put them onto 100uL Bacillus Subtilis which include LALF (plateC1 ) and no LALF protein producing Bacillus Subtilis (plateC2).As you can see where Bacillus with LALF exist ,there wasn’t any E.coli with RFP.</p><br />
<br />
<p>8. We saw that size of colonies with LALF protein is smaller than size of colonies without LALF protein and also antibiotic didn’t changed the results.</p><br />
<br />
<p>THE SUICIDE EXPERIMENT OF E. COLI</p><br />
<br />
<p>9. IPTG was caused of suicide of E.coli.Our promoter is IPTG inducable.So when we added IPTG in the media LALF synthezised.Result of this experiment we can say that E.coli kills itself.</p><br />
<br />
<p>10. LALF find in the DNA sequence and mRNA sequence. We produced LALF protein by E. coli.</p><br />
<br />
<p>11. In conclusion, BBa_K541915, BBa_K541545, BBa_K541515, BBa_K541596, BBa_K541800, BBa_K541501, BBa_K541502, BBa_K541503, BBa_K541504, BBa_K541505, BBa_K541506, BBa_K541516, BBa_K541526, BBa_K541546 are confirmed. </p><br />
<br />
<p>12. LALF find in the DNA sequence and mRNA sequence. We produced LALF protein by E. coli.</p><br />
<br />
<p>13. Producing of expected part’s mRNA is confirmed by electrophoresis and sequence analysis.<br />
As a result, mRNA of LALF protein has been produced, the promoter is producing both LALF and GFP sequences.</p><br />
<br />
<p>14. Producing of expected part’s mRNA is confirmed by electrophoresis and sequence analysis.<br />
As a result, mRNA of LALF protein has been produced, the promoter is producing both LALF and GFP sequences.</p><br />
<br />
<p>15. After adding the FR solutions on all of plates, we measured their OD values in the end of this experiment we measured again and saw that the 1st, 2nd, 3rd, 4th plates OD values didn’t change. But the the OD value of 5th plate increased. 5th plate is the control group. OFR is more effective than DFR for B,subtilis.<br />
<br />
DFR is more effective than OFR for E.coli.</p><br />
<br />
<br />
<br />
<br />
<br />
<br />
<p><strong><em></em><em>For Human Practice; We have performed the activities as follows;</em></strong></p><br />
<br />
<p>1. Providing Sporocide Method for prevention from spores of B.subtilis in further <br />
<br />
B.subtilis synthetic biology project.<em> </em></p><br />
<br />
<p>2. Preparing a novel board game `Canvas Time` to inform people from any distinct <br />
<br />
education level about features of our project, laboratory equipments and <br />
<br />
interesting Biobrick of iGEM competition.<br />
.</p><br />
<p>3.Performing open-lab to public meetings such as gifted children studying in <br />
<br />
several schools of Ankara, undergraduate students from Hacettepe University <br />
<br />
Medical School and postgraduate students from Ankara University Medical School<br />
</p><br />
<p>4.Kindergarten synthetic biology trip to teach synthetic biology to five-year-old <br />
<br />
children. <br />
</p><br />
<p><strong><br /><br />
</strong></p><br />
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Team:Fatih Turkey/Results
2011-09-22T03:51:31Z
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<p><em>1. </em><em>technique;</em> in case of applying on surfaces, LALF protein can work like a new sterilization material like gluteraldehyte or alcohol that are commonly used for hospital and lab sterilization.<em> </em></p><br />
<p><em>2. </em><em>Alternative cure for septic shock;</em> after gram negative bacterial infections; LPS, which is the main component of cell wall of gram negative bacteria, can cause deathly conditions that is generally called “septic shock”. In our project, our LALF protein binds this material strictly, thus LALF can be used as an alternative cure method for such condition.<em> </em></p><br />
<p><em>3. </em><em>Possible new structure of antibiotic-like drug; </em>antibiotics commonly effect on production of cell wall of bacteria. This is done by inhibiting the source of production. On the other hand, our LALF protein directly binds the cell wall material and stops the bacteria growth. Thus, a new antibiotic like drug made of LALF may be produced and used in some infections.<em> </em></p><br />
<p><em>4. </em><em>Possibility of producing longtime sterile layers; </em>if any kind of object is produced by covering with LALF, that object can stay sterile for long time because of gram negative bacteria protection of LALF. In case of a possible infection on surface, LALF will stop bacteria growth.<em> </em></p><br />
<p><em>5. </em><em>Alternative cure method for bacterial diseases; </em>diseases like urinary system infection or gastritis are caused by gram negative bacteria.<em> </em>In the course of applying our bacteria that produce LALF in the infectious cite, infection of harmful bacteria can be prevented or stopped. This may suggest an alternative cure for such diseases.<em> </em></p><br />
<p>On the other hand, our secondary protein, reflectin, can be used in varying applications.</p><br />
<p><em>1. </em><em>Camouflage material; </em>this protein allows its host, cephalopod, to hide when cephalopod hunts or to defend itself from its hunters. In the course of development of reflectin protein, a new camouflage material may be produced for humans.<em> </em></p><br />
<p><em>2. </em><em>New material of dye for varying applications; </em>Dye that changes its color can be used almost every colorful application. From fireworks to wall paints, from windows of buildings to road signs, this ability can be very useful.<em></em></p><br />
<p><em>3. </em><em>Possibility of producing specialized light colors; </em>in some circumstances, a unique light color may be necessary in order to gain maximum output like in greenhouses or dark rooms in photography industry. Reflectin reflects the light by changing its wavelength; therefore the light that is reflected has only one color. This ability can be applied in such application that mentioned above.<em></em></p><br />
<p><strong><br /><br />
</strong></p><br />
<br />
<p>COLLABORATION </p><br />
<br />
<p>With friendly communications teams can take some useful advises and learn some <br />
<br />
important points. Also with helping to other groups friendly feelings increase <br />
<br />
and the team’s motivation increases. During our lab working we were always <br />
<br />
keeping touch with other teams. We were helping each other and making some <br />
<br />
activities together. </p><br />
<br />
<br />
<p>We helped to Bilkent_UNAM_Turkey team when they needed our part 1001 and they <br />
<br />
helped to us with giving LB Agar. Also the Team Michigan asked helping to us <br />
<br />
about board game. We designed the Canvas Town Game and the Team Michigan wanted <br />
<br />
some points about preparing and designing. And we were being glad to help them.<br />
Association of Synthetic Biology – METU-Ankara Team invited our team to <br />
<br />
Synthetic Biology Turkey Meetings 1.0 of Igem Turkey Teams. This meeting was <br />
<br />
really funny and informative. </p><br />
<br />
<br />
<p>We are planning a little tournament of Canvas Town Game in the Asia Religion <br />
<br />
Jamboree. For this organization we help from Religion Asia Organization <br />
<br />
Committee and they helped us very quickly and politely.</p><br />
<br />
<br />
<p>For the tournament four teams will determine with drawing. 2 teams will play <br />
<br />
together and the other 2 teams will play together. Just 6 persons can play our <br />
<br />
game so every team should play with their 3 members. Then the winner teams will <br />
<br />
play together in the end of this game 3 finalist member of the last winner team <br />
<br />
will be presented.</p><br />
<br />
<br />
<p>With these activities we were really enjoyed and informed.</p><br />
<br />
<br />
<br />
<p><div><br />
<br />
<div><br />
<div class="meta-left-full"> <br />
<p><strong><em></em><em>For our project lab executions;</em></strong></p><br />
<br />
<p> 1. LALF based anti-LPS factor system has been worked expectedly<em> </em></p><br />
<p><em>2.we have constructed and submitted 12 biobrick. Three devices working in two for <br />
<br />
B.subtilis one for E.coli work expectedly.<br />
<em> </em></p><br />
<p><em>3. Our B.subtilis specific promoter with SacB- extracellur signal- has been seen <br />
<br />
working well.<em> </em></p><br />
<br />
<p><em>4. We have synthesized Reflectin 1a protein in E.coli; however, because we did not <br />
<br />
have enough time to purify it to visualize via aid-eye, we could not use <br />
<br />
Reflectin 1a device as biosensor for our lalf-LPS interaction in B.subtilis-<br />
<br />
E.coli mixed culture. <em> </em></p><br />
<br />
<p>DISC EXPERIMENT1</p><br />
<br />
<p>5. There are b.subtilis with LALF in our B1 plate and B.subtilis without LALF in our B2 plate.For working of our LALF protein ,count of e.coli with RFP in B1 is lower than B2.</p><br />
<br />
<br />
<p>6. We spread fresh E.coli which produce RFP and put them onto 100uL Bacillus Subtilis supernatant which include LALF (plate D1) and no LALF protein producing Bacillus Subtilis supernatant(plateD2).As you can see where Bacillus supernatant with LALF exist ,there wasn’t any E.coli with RFP.</p><br />
<br />
<br />
<p>7. We spread fresh E.coli which produce RFP and put them onto 100uL Bacillus Subtilis which include LALF (plateC1 ) and no LALF protein producing Bacillus Subtilis (plateC2).As you can see where Bacillus with LALF exist ,there wasn’t any E.coli with RFP.</p><br />
<br />
<p>8. We saw that size of colonies with LALF protein is smaller than size of colonies without LALF protein and also antibiotic didn’t changed the results.</p><br />
<br />
<p>THE SUICIDE EXPERIMENT OF E. COLI</p><br />
<br />
<p>9. IPTG was caused of suicide of E.coli.Our promoter is IPTG inducable.So when we added IPTG in the media LALF synthezised.Result of this experiment we can say that E.coli kills itself.</p><br />
<br />
<br />
<br />
<p><strong><em></em><em>For Human Practice; We have performed the activities as follows;</em></strong></p><br />
<br />
<p>1. Providing Sporocide Method for prevention from spores of B.subtilis in further <br />
<br />
B.subtilis synthetic biology project.<em> </em></p><br />
<br />
<p>2. Preparing a novel board game `Canvas Time` to inform people from any distinct <br />
<br />
education level about features of our project, laboratory equipments and <br />
<br />
interesting Biobrick of iGEM competition.<br />
.</p><br />
<p>3.Performing open-lab to public meetings such as gifted children studying in <br />
<br />
several schools of Ankara, undergraduate students from Hacettepe University <br />
<br />
Medical School and postgraduate students from Ankara University Medical School<br />
</p><br />
<p>4.Kindergarten synthetic biology trip to teach synthetic biology to five-year-old <br />
<br />
children. <br />
</p><br />
<p><strong><br /><br />
</strong></p><br />
<br />
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Team:Fatih Turkey/Results
2011-09-22T03:50:29Z
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<h2 style="font-family: Verdana, Arial, SunSans-Regular, sans-serif;">Project</h2><br />
<h5 style="font-family: Verdana, Arial, SunSans-Regular, sans-serif;">Results</h5> <br />
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<p><em>1. </em><em>technique;</em> in case of applying on surfaces, LALF protein can work like a new sterilization material like gluteraldehyte or alcohol that are commonly used for hospital and lab sterilization.<em> </em></p><br />
<p><em>2. </em><em>Alternative cure for septic shock;</em> after gram negative bacterial infections; LPS, which is the main component of cell wall of gram negative bacteria, can cause deathly conditions that is generally called “septic shock”. In our project, our LALF protein binds this material strictly, thus LALF can be used as an alternative cure method for such condition.<em> </em></p><br />
<p><em>3. </em><em>Possible new structure of antibiotic-like drug; </em>antibiotics commonly effect on production of cell wall of bacteria. This is done by inhibiting the source of production. On the other hand, our LALF protein directly binds the cell wall material and stops the bacteria growth. Thus, a new antibiotic like drug made of LALF may be produced and used in some infections.<em> </em></p><br />
<p><em>4. </em><em>Possibility of producing longtime sterile layers; </em>if any kind of object is produced by covering with LALF, that object can stay sterile for long time because of gram negative bacteria protection of LALF. In case of a possible infection on surface, LALF will stop bacteria growth.<em> </em></p><br />
<p><em>5. </em><em>Alternative cure method for bacterial diseases; </em>diseases like urinary system infection or gastritis are caused by gram negative bacteria.<em> </em>In the course of applying our bacteria that produce LALF in the infectious cite, infection of harmful bacteria can be prevented or stopped. This may suggest an alternative cure for such diseases.<em> </em></p><br />
<p>On the other hand, our secondary protein, reflectin, can be used in varying applications.</p><br />
<p><em>1. </em><em>Camouflage material; </em>this protein allows its host, cephalopod, to hide when cephalopod hunts or to defend itself from its hunters. In the course of development of reflectin protein, a new camouflage material may be produced for humans.<em> </em></p><br />
<p><em>2. </em><em>New material of dye for varying applications; </em>Dye that changes its color can be used almost every colorful application. From fireworks to wall paints, from windows of buildings to road signs, this ability can be very useful.<em></em></p><br />
<p><em>3. </em><em>Possibility of producing specialized light colors; </em>in some circumstances, a unique light color may be necessary in order to gain maximum output like in greenhouses or dark rooms in photography industry. Reflectin reflects the light by changing its wavelength; therefore the light that is reflected has only one color. This ability can be applied in such application that mentioned above.<em></em></p><br />
<p><strong><br /><br />
</strong></p><br />
<br />
<p>COLLABORATION </p><br />
<br />
<p>With friendly communications teams can take some useful advises and learn some <br />
<br />
important points. Also with helping to other groups friendly feelings increase <br />
<br />
and the team’s motivation increases. During our lab working we were always <br />
<br />
keeping touch with other teams. We were helping each other and making some <br />
<br />
activities together. </p><br />
<br />
<br />
<p>We helped to Bilkent_UNAM_Turkey team when they needed our part 1001 and they <br />
<br />
helped to us with giving LB Agar. Also the Team Michigan asked helping to us <br />
<br />
about board game. We designed the Canvas Town Game and the Team Michigan wanted <br />
<br />
some points about preparing and designing. And we were being glad to help them.<br />
Association of Synthetic Biology – METU-Ankara Team invited our team to <br />
<br />
Synthetic Biology Turkey Meetings 1.0 of Igem Turkey Teams. This meeting was <br />
<br />
really funny and informative. </p><br />
<br />
<br />
<p>We are planning a little tournament of Canvas Town Game in the Asia Religion <br />
<br />
Jamboree. For this organization we help from Religion Asia Organization <br />
<br />
Committee and they helped us very quickly and politely.</p><br />
<br />
<br />
<p>For the tournament four teams will determine with drawing. 2 teams will play <br />
<br />
together and the other 2 teams will play together. Just 6 persons can play our <br />
<br />
game so every team should play with their 3 members. Then the winner teams will <br />
<br />
play together in the end of this game 3 finalist member of the last winner team <br />
<br />
will be presented.</p><br />
<br />
<br />
<p>With these activities we were really enjoyed and informed.</p><br />
<br />
<br />
<br />
<p><div><br />
<br />
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<p><strong><em></em><em>For our project lab executions;</em></strong></p><br />
<br />
<p> 1. LALF based anti-LPS factor system has been worked expectedly<em> </em></p><br />
<p><em>2.we have constructed and submitted 12 biobrick. Three devices working in two for <br />
<br />
B.subtilis one for E.coli work expectedly.<br />
<em> </em></p><br />
<p><em>3. Our B.subtilis specific promoter with SacB- extracellur signal- has been seen <br />
<br />
working well.<em> </em></p><br />
<br />
<p><em>4. We have synthesized Reflectin 1a protein in E.coli; however, because we did not <br />
<br />
have enough time to purify it to visualize via aid-eye, we could not use <br />
<br />
Reflectin 1a device as biosensor for our lalf-LPS interaction in B.subtilis-<br />
<br />
E.coli mixed culture. <em> </em></p><br />
<br />
<p>Disc Experiment1</p><br />
<br />
<p>5. There are b.subtilis with LALF in our B1 plate and B.subtilis without LALF in our B2 plate.For working of our LALF protein ,count of e.coli with RFP in B1 is lower than B2.</p><br />
<br />
<br />
<p>6. We spread fresh E.coli which produce RFP and put them onto 100uL Bacillus Subtilis supernatant which include LALF (plate D1) and no LALF protein producing Bacillus Subtilis supernatant(plateD2).As you can see where Bacillus supernatant with LALF exist ,there wasn’t any E.coli with RFP.</p><br />
<br />
<br />
<p>7. We spread fresh E.coli which produce RFP and put them onto 100uL Bacillus Subtilis which include LALF (plateC1 ) and no LALF protein producing Bacillus Subtilis (plateC2).As you can see where Bacillus with LALF exist ,there wasn’t any E.coli with RFP.</p><br />
<br />
<p>8. We saw that size of colonies with LALF protein is smaller than size of colonies without LALF protein and also antibiotic didn’t changed the results.</p><br />
<br />
<p>THE SUICIDE EXPERIMENT OF E. COLI</p><br />
<br />
<p>9. IPTG was caused of suicide of E.coli.Our promoter is IPTG inducable.So when we added IPTG in the media LALF synthezised.Result of this experiment we can say that E.coli kills itself.</p><br />
<br />
<br />
<br />
<p><strong><em></em><em>For Human Practice; We have performed the activities as follows;</em></strong></p><br />
<br />
<p>1. Providing Sporocide Method for prevention from spores of B.subtilis in further <br />
<br />
B.subtilis synthetic biology project.<em> </em></p><br />
<br />
<p>2. Preparing a novel board game `Canvas Time` to inform people from any distinct <br />
<br />
education level about features of our project, laboratory equipments and <br />
<br />
interesting Biobrick of iGEM competition.<br />
.</p><br />
<p>3.Performing open-lab to public meetings such as gifted children studying in <br />
<br />
several schools of Ankara, undergraduate students from Hacettepe University <br />
<br />
Medical School and postgraduate students from Ankara University Medical School<br />
</p><br />
<p>4.Kindergarten synthetic biology trip to teach synthetic biology to five-year-old <br />
<br />
children. <br />
</p><br />
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Team:Fatih Turkey/Results
2011-09-22T03:48:46Z
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<h2 style="font-family: Verdana, Arial, SunSans-Regular, sans-serif;">Project</h2><br />
<h5 style="font-family: Verdana, Arial, SunSans-Regular, sans-serif;">Results</h5> <br />
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<p><em>1. </em><em>technique;</em> in case of applying on surfaces, LALF protein can work like a new sterilization material like gluteraldehyte or alcohol that are commonly used for hospital and lab sterilization.<em> </em></p><br />
<p><em>2. </em><em>Alternative cure for septic shock;</em> after gram negative bacterial infections; LPS, which is the main component of cell wall of gram negative bacteria, can cause deathly conditions that is generally called “septic shock”. In our project, our LALF protein binds this material strictly, thus LALF can be used as an alternative cure method for such condition.<em> </em></p><br />
<p><em>3. </em><em>Possible new structure of antibiotic-like drug; </em>antibiotics commonly effect on production of cell wall of bacteria. This is done by inhibiting the source of production. On the other hand, our LALF protein directly binds the cell wall material and stops the bacteria growth. Thus, a new antibiotic like drug made of LALF may be produced and used in some infections.<em> </em></p><br />
<p><em>4. </em><em>Possibility of producing longtime sterile layers; </em>if any kind of object is produced by covering with LALF, that object can stay sterile for long time because of gram negative bacteria protection of LALF. In case of a possible infection on surface, LALF will stop bacteria growth.<em> </em></p><br />
<p><em>5. </em><em>Alternative cure method for bacterial diseases; </em>diseases like urinary system infection or gastritis are caused by gram negative bacteria.<em> </em>In the course of applying our bacteria that produce LALF in the infectious cite, infection of harmful bacteria can be prevented or stopped. This may suggest an alternative cure for such diseases.<em> </em></p><br />
<p>On the other hand, our secondary protein, reflectin, can be used in varying applications.</p><br />
<p><em>1. </em><em>Camouflage material; </em>this protein allows its host, cephalopod, to hide when cephalopod hunts or to defend itself from its hunters. In the course of development of reflectin protein, a new camouflage material may be produced for humans.<em> </em></p><br />
<p><em>2. </em><em>New material of dye for varying applications; </em>Dye that changes its color can be used almost every colorful application. From fireworks to wall paints, from windows of buildings to road signs, this ability can be very useful.<em></em></p><br />
<p><em>3. </em><em>Possibility of producing specialized light colors; </em>in some circumstances, a unique light color may be necessary in order to gain maximum output like in greenhouses or dark rooms in photography industry. Reflectin reflects the light by changing its wavelength; therefore the light that is reflected has only one color. This ability can be applied in such application that mentioned above.<em></em></p><br />
<p><strong><br /><br />
</strong></p><br />
<br />
<p>COLLABORATION </p><br />
<br />
<p>With friendly communications teams can take some useful advises and learn some <br />
<br />
important points. Also with helping to other groups friendly feelings increase <br />
<br />
and the team’s motivation increases. During our lab working we were always <br />
<br />
keeping touch with other teams. We were helping each other and making some <br />
<br />
activities together. </p><br />
<br />
<br />
<p>We helped to Bilkent_UNAM_Turkey team when they needed our part 1001 and they <br />
<br />
helped to us with giving LB Agar. Also the Team Michigan asked helping to us <br />
<br />
about board game. We designed the Canvas Town Game and the Team Michigan wanted <br />
<br />
some points about preparing and designing. And we were being glad to help them.<br />
Association of Synthetic Biology – METU-Ankara Team invited our team to <br />
<br />
Synthetic Biology Turkey Meetings 1.0 of Igem Turkey Teams. This meeting was <br />
<br />
really funny and informative. </p><br />
<br />
<br />
<p>We are planning a little tournament of Canvas Town Game in the Asia Religion <br />
<br />
Jamboree. For this organization we help from Religion Asia Organization <br />
<br />
Committee and they helped us very quickly and politely.</p><br />
<br />
<br />
<p>For the tournament four teams will determine with drawing. 2 teams will play <br />
<br />
together and the other 2 teams will play together. Just 6 persons can play our <br />
<br />
game so every team should play with their 3 members. Then the winner teams will <br />
<br />
play together in the end of this game 3 finalist member of the last winner team <br />
<br />
will be presented.</p><br />
<br />
<br />
<p>With these activities we were really enjoyed and informed.</p><br />
<br />
<br />
<br />
<p><div><br />
<br />
<div><br />
<div class="meta-left-full"> <br />
<p><strong><em></em><em>For our project lab executions;</em></strong></p><br />
<br />
<p> 1. LALF based anti-LPS factor system has been worked expectedly<em> </em></p><br />
<p><em>2.we have constructed and submitted 12 biobrick. Three devices working in two for <br />
<br />
B.subtilis one for E.coli work expectedly.<br />
<em> </em></p><br />
<p><em>3. Our B.subtilis specific promoter with SacB- extracellur signal- has been seen <br />
<br />
working well.<em> </em></p><br />
<br />
<p><em>4. We have synthesized Reflectin 1a protein in E.coli; however, because we did not <br />
<br />
have enough time to purify it to visualize via aid-eye, we could not use <br />
<br />
Reflectin 1a device as biosensor for our lalf-LPS interaction in B.subtilis-<br />
<br />
E.coli mixed culture. <em> </em></p><br />
<br />
<p>Disc Experiment1</p><br />
<br />
<p>There are b.subtilis with LALF in our B1 plate and b.subtilis without LALF in our B2 plate.For working of our LALF protein ,count of e.coli with RFP in B1 is lower than B2.</p><br />
<br />
<br />
<p>We spread fresh E.coli which produce RFP and put them onto 100uL Bacillus Subtilis supernatant which include LALF (plate D1) and no LALF protein producing Bacillus Subtilis supernatant(plateD2).As you can see where Bacillus supernatant with LALF exist ,there wasn’t any E.coli with RFP.</p><br />
<br />
<br />
<p>We spread fresh E.coli which produce RFP and put them onto 100uL Bacillus Subtilis which include LALF (plateC1 ) and no LALF protein producing Bacillus Subtilis (plateC2).As you can see where Bacillus with LALF exist ,there wasn’t any E.coli with RFP.</p><br />
<br />
<p>We saw that size of colonies with LALF protein is smaller than size of colonies without LALF protein and also antibiotic didn’t changed the results.</p><br />
<br />
<p>THE SUICIDE EXPERIMENT OF E. COLI</p><br />
<br />
<p>IPTG was caused of suicide of E.coli.Our promoter is IPTG inducable.So when we added IPTG in the media LALF synthezised.Result of this experiment we can say that E.coli kills itself.</p><br />
<br />
<br />
<br />
<p><strong><em></em><em>For Human Practice; We have performed the activities as follows;</em></strong></p><br />
<br />
<p>1. Providing Sporocide Method for prevention from spores of B.subtilis in further <br />
<br />
B.subtilis synthetic biology project.<em> </em></p><br />
<br />
<p>2. Preparing a novel board game `Canvas Time` to inform people from any distinct <br />
<br />
education level about features of our project, laboratory equipments and <br />
<br />
interesting Biobrick of iGEM competition.<br />
.</p><br />
<p>3.Performing open-lab to public meetings such as gifted children studying in <br />
<br />
several schools of Ankara, undergraduate students from Hacettepe University <br />
<br />
Medical School and postgraduate students from Ankara University Medical School<br />
</p><br />
<p>4.Kindergarten synthetic biology trip to teach synthetic biology to five-year-old <br />
<br />
children. <br />
</p><br />
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http://2011.igem.org/Team:Fatih_Turkey/Results
Team:Fatih Turkey/Results
2011-09-22T03:47:55Z
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<h2 style="font-family: Verdana, Arial, SunSans-Regular, sans-serif;">Project</h2><br />
<h5 style="font-family: Verdana, Arial, SunSans-Regular, sans-serif;">Results</h5> <br />
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<p><em>1. </em><em>technique;</em> in case of applying on surfaces, LALF protein can work like a new sterilization material like gluteraldehyte or alcohol that are commonly used for hospital and lab sterilization.<em> </em></p><br />
<p><em>2. </em><em>Alternative cure for septic shock;</em> after gram negative bacterial infections; LPS, which is the main component of cell wall of gram negative bacteria, can cause deathly conditions that is generally called “septic shock”. In our project, our LALF protein binds this material strictly, thus LALF can be used as an alternative cure method for such condition.<em> </em></p><br />
<p><em>3. </em><em>Possible new structure of antibiotic-like drug; </em>antibiotics commonly effect on production of cell wall of bacteria. This is done by inhibiting the source of production. On the other hand, our LALF protein directly binds the cell wall material and stops the bacteria growth. Thus, a new antibiotic like drug made of LALF may be produced and used in some infections.<em> </em></p><br />
<p><em>4. </em><em>Possibility of producing longtime sterile layers; </em>if any kind of object is produced by covering with LALF, that object can stay sterile for long time because of gram negative bacteria protection of LALF. In case of a possible infection on surface, LALF will stop bacteria growth.<em> </em></p><br />
<p><em>5. </em><em>Alternative cure method for bacterial diseases; </em>diseases like urinary system infection or gastritis are caused by gram negative bacteria.<em> </em>In the course of applying our bacteria that produce LALF in the infectious cite, infection of harmful bacteria can be prevented or stopped. This may suggest an alternative cure for such diseases.<em> </em></p><br />
<p>On the other hand, our secondary protein, reflectin, can be used in varying applications.</p><br />
<p><em>1. </em><em>Camouflage material; </em>this protein allows its host, cephalopod, to hide when cephalopod hunts or to defend itself from its hunters. In the course of development of reflectin protein, a new camouflage material may be produced for humans.<em> </em></p><br />
<p><em>2. </em><em>New material of dye for varying applications; </em>Dye that changes its color can be used almost every colorful application. From fireworks to wall paints, from windows of buildings to road signs, this ability can be very useful.<em></em></p><br />
<p><em>3. </em><em>Possibility of producing specialized light colors; </em>in some circumstances, a unique light color may be necessary in order to gain maximum output like in greenhouses or dark rooms in photography industry. Reflectin reflects the light by changing its wavelength; therefore the light that is reflected has only one color. This ability can be applied in such application that mentioned above.<em></em></p><br />
<p><strong><br /><br />
</strong></p><br />
<br />
<p>COLLABORATION </p><br />
<br />
<p>With friendly communications teams can take some useful advises and learn some <br />
<br />
important points. Also with helping to other groups friendly feelings increase <br />
<br />
and the team’s motivation increases. During our lab working we were always <br />
<br />
keeping touch with other teams. We were helping each other and making some <br />
<br />
activities together. </p><br />
<br />
<br />
<p>We helped to Bilkent_UNAM_Turkey team when they needed our part 1001 and they <br />
<br />
helped to us with giving LB Agar. Also the Team Michigan asked helping to us <br />
<br />
about board game. We designed the Canvas Town Game and the Team Michigan wanted <br />
<br />
some points about preparing and designing. And we were being glad to help them.<br />
Association of Synthetic Biology – METU-Ankara Team invited our team to <br />
<br />
Synthetic Biology Turkey Meetings 1.0 of Igem Turkey Teams. This meeting was <br />
<br />
really funny and informative. </p><br />
<br />
<br />
<p>We are planning a little tournament of Canvas Town Game in the Asia Religion <br />
<br />
Jamboree. For this organization we help from Religion Asia Organization <br />
<br />
Committee and they helped us very quickly and politely.</p><br />
<br />
<br />
<p>For the tournament four teams will determine with drawing. 2 teams will play <br />
<br />
together and the other 2 teams will play together. Just 6 persons can play our <br />
<br />
game so every team should play with their 3 members. Then the winner teams will <br />
<br />
play together in the end of this game 3 finalist member of the last winner team <br />
<br />
will be presented.</p><br />
<br />
<br />
<p>With these activities we were really enjoyed and informed.</p><br />
<br />
<br />
<br />
<p><div><br />
<br />
<div><br />
<div class="meta-left-full"> <br />
<p><strong><em></em><em>For our project lab executions;</em></strong></p><br />
<br />
<p> 1. LALF based anti-LPS factor system has been worked expectedly<em> </em></p><br />
<p><em>2.we have constructed and submitted 12 biobrick. Three devices working in two for <br />
<br />
B.subtilis one for E.coli work expectedly.<br />
<em> </em></p><br />
<p><em>3. Our B.subtilis specific promoter with SacB- extracellur signal- has been seen <br />
<br />
working well.<em> </em></p><br />
<br />
<p><em>4. We have synthesized Reflectin 1a protein in E.coli; however, because we did not <br />
<br />
have enough time to purify it to visualize via aid-eye, we could not use <br />
<br />
Reflectin 1a device as biosensor for our lalf-LPS interaction in B.subtilis-<br />
<br />
E.coli mixed culture. <em> </em></p><br />
<br />
<p>Disc Experiment1</p><br />
<br />
<html>https://static.igem.org/mediawiki/2011/d/d1/Sss1.png</html><br />
<br />
<p>There are b.subtilis with LALF in our B1 plate and b.subtilis without LALF in our B2 plate.For working of our LALF protein ,count of e.coli with RFP in B1 is lower than B2.</p><br />
<br />
https://static.igem.org/mediawiki/igem.org/f/fd/Sss2.png<br />
<br />
<p>We spread fresh E.coli which produce RFP and put them onto 100uL Bacillus Subtilis supernatant which include LALF (plate D1) and no LALF protein producing Bacillus Subtilis supernatant(plateD2).As you can see where Bacillus supernatant with LALF exist ,there wasn’t any E.coli with RFP.</p><br />
<br />
https://static.igem.org/mediawiki/2011/8/85/Sss3.png<br />
<br />
<p>We spread fresh E.coli which produce RFP and put them onto 100uL Bacillus Subtilis which include LALF (plateC1 ) and no LALF protein producing Bacillus Subtilis (plateC2).As you can see where Bacillus with LALF exist ,there wasn’t any E.coli with RFP.</p><br />
<br />
<br />
https://static.igem.org/mediawiki/2011/f/f9/IMG_0331.JPG<br />
https://static.igem.org/mediawiki/2011/1/1f/IMG_0335.JPG<br />
https://static.igem.org/mediawiki/2011/a/a6/IMG_0339.JPG<br />
We saw that size of colonies with LALF protein is smaller than size of colonies without LALF protein and also antibiotic didn’t changed the results.<br />
<br />
<p>THE SUICIDE EXPERIMENT OF E. COLI</p><br />
<br />
<p>IPTG was caused of suicide of E.coli.Our promoter is IPTG inducable.So when we added IPTG in the media LALF synthezised.Result of this experiment we can say that E.coli kills itself.</p><br />
<br />
<br />
<br />
<p><strong><em></em><em>For Human Practice; We have performed the activities as follows;</em></strong></p><br />
<br />
<p>1. Providing Sporocide Method for prevention from spores of B.subtilis in further <br />
<br />
B.subtilis synthetic biology project.<em> </em></p><br />
<br />
<p>2. Preparing a novel board game `Canvas Time` to inform people from any distinct <br />
<br />
education level about features of our project, laboratory equipments and <br />
<br />
interesting Biobrick of iGEM competition.<br />
.</p><br />
<p>3.Performing open-lab to public meetings such as gifted children studying in <br />
<br />
several schools of Ankara, undergraduate students from Hacettepe University <br />
<br />
Medical School and postgraduate students from Ankara University Medical School<br />
</p><br />
<p>4.Kindergarten synthetic biology trip to teach synthetic biology to five-year-old <br />
<br />
children. <br />
</p><br />
<p><strong><br /><br />
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Metugen
http://2011.igem.org/Team:Fatih_Turkey/Results
Team:Fatih Turkey/Results
2011-09-22T03:46:55Z
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<h2 style="font-family: Verdana, Arial, SunSans-Regular, sans-serif;">Project</h2><br />
<h5 style="font-family: Verdana, Arial, SunSans-Regular, sans-serif;">Results</h5> <br />
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<p><em>1. </em><em>technique;</em> in case of applying on surfaces, LALF protein can work like a new sterilization material like gluteraldehyte or alcohol that are commonly used for hospital and lab sterilization.<em> </em></p><br />
<p><em>2. </em><em>Alternative cure for septic shock;</em> after gram negative bacterial infections; LPS, which is the main component of cell wall of gram negative bacteria, can cause deathly conditions that is generally called “septic shock”. In our project, our LALF protein binds this material strictly, thus LALF can be used as an alternative cure method for such condition.<em> </em></p><br />
<p><em>3. </em><em>Possible new structure of antibiotic-like drug; </em>antibiotics commonly effect on production of cell wall of bacteria. This is done by inhibiting the source of production. On the other hand, our LALF protein directly binds the cell wall material and stops the bacteria growth. Thus, a new antibiotic like drug made of LALF may be produced and used in some infections.<em> </em></p><br />
<p><em>4. </em><em>Possibility of producing longtime sterile layers; </em>if any kind of object is produced by covering with LALF, that object can stay sterile for long time because of gram negative bacteria protection of LALF. In case of a possible infection on surface, LALF will stop bacteria growth.<em> </em></p><br />
<p><em>5. </em><em>Alternative cure method for bacterial diseases; </em>diseases like urinary system infection or gastritis are caused by gram negative bacteria.<em> </em>In the course of applying our bacteria that produce LALF in the infectious cite, infection of harmful bacteria can be prevented or stopped. This may suggest an alternative cure for such diseases.<em> </em></p><br />
<p>On the other hand, our secondary protein, reflectin, can be used in varying applications.</p><br />
<p><em>1. </em><em>Camouflage material; </em>this protein allows its host, cephalopod, to hide when cephalopod hunts or to defend itself from its hunters. In the course of development of reflectin protein, a new camouflage material may be produced for humans.<em> </em></p><br />
<p><em>2. </em><em>New material of dye for varying applications; </em>Dye that changes its color can be used almost every colorful application. From fireworks to wall paints, from windows of buildings to road signs, this ability can be very useful.<em></em></p><br />
<p><em>3. </em><em>Possibility of producing specialized light colors; </em>in some circumstances, a unique light color may be necessary in order to gain maximum output like in greenhouses or dark rooms in photography industry. Reflectin reflects the light by changing its wavelength; therefore the light that is reflected has only one color. This ability can be applied in such application that mentioned above.<em></em></p><br />
<p><strong><br /><br />
</strong></p><br />
<br />
<p>COLLABORATION </p><br />
<br />
<p>With friendly communications teams can take some useful advises and learn some <br />
<br />
important points. Also with helping to other groups friendly feelings increase <br />
<br />
and the team’s motivation increases. During our lab working we were always <br />
<br />
keeping touch with other teams. We were helping each other and making some <br />
<br />
activities together. </p><br />
<br />
<br />
<p>We helped to Bilkent_UNAM_Turkey team when they needed our part 1001 and they <br />
<br />
helped to us with giving LB Agar. Also the Team Michigan asked helping to us <br />
<br />
about board game. We designed the Canvas Town Game and the Team Michigan wanted <br />
<br />
some points about preparing and designing. And we were being glad to help them.<br />
Association of Synthetic Biology – METU-Ankara Team invited our team to <br />
<br />
Synthetic Biology Turkey Meetings 1.0 of Igem Turkey Teams. This meeting was <br />
<br />
really funny and informative. </p><br />
<br />
<br />
<p>We are planning a little tournament of Canvas Town Game in the Asia Religion <br />
<br />
Jamboree. For this organization we help from Religion Asia Organization <br />
<br />
Committee and they helped us very quickly and politely.</p><br />
<br />
<br />
<p>For the tournament four teams will determine with drawing. 2 teams will play <br />
<br />
together and the other 2 teams will play together. Just 6 persons can play our <br />
<br />
game so every team should play with their 3 members. Then the winner teams will <br />
<br />
play together in the end of this game 3 finalist member of the last winner team <br />
<br />
will be presented.</p><br />
<br />
<br />
<p>With these activities we were really enjoyed and informed.</p><br />
<br />
<br />
<br />
<p><div><br />
<br />
<div><br />
<div class="meta-left-full"> <br />
<p><strong><em></em><em>For our project lab executions;</em></strong></p><br />
<br />
<p> 1. LALF based anti-LPS factor system has been worked expectedly<em> </em></p><br />
<p><em>2.we have constructed and submitted 12 biobrick. Three devices working in two for <br />
<br />
B.subtilis one for E.coli work expectedly.<br />
<em> </em></p><br />
<p><em>3. Our B.subtilis specific promoter with SacB- extracellur signal- has been seen <br />
<br />
working well.<em> </em></p><br />
<br />
<p><em>4. We have synthesized Reflectin 1a protein in E.coli; however, because we did not <br />
<br />
have enough time to purify it to visualize via aid-eye, we could not use <br />
<br />
Reflectin 1a device as biosensor for our lalf-LPS interaction in B.subtilis-<br />
<br />
E.coli mixed culture. <em> </em></p><br />
<br />
<p>Disc deneyi1</p><br />
<br />
https://static.igem.org/mediawiki/2011/d/d1/Sss1.png<br />
<br />
<p>There are b.subtilis with LALF in our B1 plate and b.subtilis without LALF in our B2 plate.For working of our LALF protein ,count of e.coli with RFP in B1 is lower than B2.</p><br />
<br />
https://static.igem.org/mediawiki/igem.org/f/fd/Sss2.png<br />
<br />
<p>We spread fresh E.coli which produce RFP and put them onto 100uL Bacillus Subtilis supernatant which include LALF (plate D1) and no LALF protein producing Bacillus Subtilis supernatant(plateD2).As you can see where Bacillus supernatant with LALF exist ,there wasn’t any E.coli with RFP.</p><br />
<br />
https://static.igem.org/mediawiki/2011/8/85/Sss3.png<br />
<br />
<p>We spread fresh E.coli which produce RFP and put them onto 100uL Bacillus Subtilis which include LALF (plateC1 ) and no LALF protein producing Bacillus Subtilis (plateC2).As you can see where Bacillus with LALF exist ,there wasn’t any E.coli with RFP.</p><br />
<br />
<br />
https://static.igem.org/mediawiki/2011/f/f9/IMG_0331.JPG<br />
https://static.igem.org/mediawiki/2011/1/1f/IMG_0335.JPG<br />
https://static.igem.org/mediawiki/2011/a/a6/IMG_0339.JPG<br />
We saw that size of colonies with LALF protein is smaller than size of colonies without LALF protein and also antibiotic didn’t changed the results.<br />
<br />
<p>THE SUICIDE EXPERIMENT OF E. COLI</p><br />
<br />
<p>IPTG was caused of suicide of E.coli.Our promoter is IPTG inducable.So when we added IPTG in the media LALF synthezised.Result of this experiment we can say that E.coli kills itself.</p><br />
<br />
<br />
<br />
<p><strong><em></em><em>For Human Practice; We have performed the activities as follows;</em></strong></p><br />
<br />
<p>1. Providing Sporocide Method for prevention from spores of B.subtilis in further <br />
<br />
B.subtilis synthetic biology project.<em> </em></p><br />
<br />
<p>2. Preparing a novel board game `Canvas Time` to inform people from any distinct <br />
<br />
education level about features of our project, laboratory equipments and <br />
<br />
interesting Biobrick of iGEM competition.<br />
.</p><br />
<p>3.Performing open-lab to public meetings such as gifted children studying in <br />
<br />
several schools of Ankara, undergraduate students from Hacettepe University <br />
<br />
Medical School and postgraduate students from Ankara University Medical School<br />
</p><br />
<p>4.Kindergarten synthetic biology trip to teach synthetic biology to five-year-old <br />
<br />
children. <br />
</p><br />
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Metugen
http://2011.igem.org/Team:Fatih_Turkey/Results
Team:Fatih Turkey/Results
2011-09-22T03:46:00Z
<p>Metugen: </p>
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<h2 style="font-family: Verdana, Arial, SunSans-Regular, sans-serif;">Project</h2><br />
<h5 style="font-family: Verdana, Arial, SunSans-Regular, sans-serif;">Results</h5> <br />
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<p><em>1. </em><em>technique;</em> in case of applying on surfaces, LALF protein can work like a new sterilization material like gluteraldehyte or alcohol that are commonly used for hospital and lab sterilization.<em> </em></p><br />
<p><em>2. </em><em>Alternative cure for septic shock;</em> after gram negative bacterial infections; LPS, which is the main component of cell wall of gram negative bacteria, can cause deathly conditions that is generally called “septic shock”. In our project, our LALF protein binds this material strictly, thus LALF can be used as an alternative cure method for such condition.<em> </em></p><br />
<p><em>3. </em><em>Possible new structure of antibiotic-like drug; </em>antibiotics commonly effect on production of cell wall of bacteria. This is done by inhibiting the source of production. On the other hand, our LALF protein directly binds the cell wall material and stops the bacteria growth. Thus, a new antibiotic like drug made of LALF may be produced and used in some infections.<em> </em></p><br />
<p><em>4. </em><em>Possibility of producing longtime sterile layers; </em>if any kind of object is produced by covering with LALF, that object can stay sterile for long time because of gram negative bacteria protection of LALF. In case of a possible infection on surface, LALF will stop bacteria growth.<em> </em></p><br />
<p><em>5. </em><em>Alternative cure method for bacterial diseases; </em>diseases like urinary system infection or gastritis are caused by gram negative bacteria.<em> </em>In the course of applying our bacteria that produce LALF in the infectious cite, infection of harmful bacteria can be prevented or stopped. This may suggest an alternative cure for such diseases.<em> </em></p><br />
<p>On the other hand, our secondary protein, reflectin, can be used in varying applications.</p><br />
<p><em>1. </em><em>Camouflage material; </em>this protein allows its host, cephalopod, to hide when cephalopod hunts or to defend itself from its hunters. In the course of development of reflectin protein, a new camouflage material may be produced for humans.<em> </em></p><br />
<p><em>2. </em><em>New material of dye for varying applications; </em>Dye that changes its color can be used almost every colorful application. From fireworks to wall paints, from windows of buildings to road signs, this ability can be very useful.<em></em></p><br />
<p><em>3. </em><em>Possibility of producing specialized light colors; </em>in some circumstances, a unique light color may be necessary in order to gain maximum output like in greenhouses or dark rooms in photography industry. Reflectin reflects the light by changing its wavelength; therefore the light that is reflected has only one color. This ability can be applied in such application that mentioned above.<em></em></p><br />
<p><strong><br /><br />
</strong></p><br />
<br />
<p>COLLABORATION </p><br />
<br />
<p>With friendly communications teams can take some useful advises and learn some <br />
<br />
important points. Also with helping to other groups friendly feelings increase <br />
<br />
and the team’s motivation increases. During our lab working we were always <br />
<br />
keeping touch with other teams. We were helping each other and making some <br />
<br />
activities together. </p><br />
<br />
<br />
<p>We helped to Bilkent_UNAM_Turkey team when they needed our part 1001 and they <br />
<br />
helped to us with giving LB Agar. Also the Team Michigan asked helping to us <br />
<br />
about board game. We designed the Canvas Town Game and the Team Michigan wanted <br />
<br />
some points about preparing and designing. And we were being glad to help them.<br />
Association of Synthetic Biology – METU-Ankara Team invited our team to <br />
<br />
Synthetic Biology Turkey Meetings 1.0 of Igem Turkey Teams. This meeting was <br />
<br />
really funny and informative. </p><br />
<br />
<br />
<p>We are planning a little tournament of Canvas Town Game in the Asia Religion <br />
<br />
Jamboree. For this organization we help from Religion Asia Organization <br />
<br />
Committee and they helped us very quickly and politely.</p><br />
<br />
<br />
<p>For the tournament four teams will determine with drawing. 2 teams will play <br />
<br />
together and the other 2 teams will play together. Just 6 persons can play our <br />
<br />
game so every team should play with their 3 members. Then the winner teams will <br />
<br />
play together in the end of this game 3 finalist member of the last winner team <br />
<br />
will be presented.</p><br />
<br />
<br />
<p>With these activities we were really enjoyed and informed.</p><br />
<br />
<br />
<br />
<p><div><br />
<br />
<div><br />
<div class="meta-left-full"> <br />
<p><strong><em></em><em>For our project lab executions;</em></strong></p><br />
<br />
<p> 1. LALF based anti-LPS factor system has been worked expectedly<em> </em></p><br />
<p><em>2.we have constructed and submitted 12 biobrick. Three devices working in two for <br />
<br />
B.subtilis one for E.coli work expectedly.<br />
<em> </em></p><br />
<p><em>3. Our B.subtilis specific promoter with SacB- extracellur signal- has been seen <br />
<br />
working well.<em> </em></p><br />
<br />
<p><em>4. We have synthesized Reflectin 1a protein in E.coli; however, because we did not <br />
<br />
have enough time to purify it to visualize via aid-eye, we could not use <br />
<br />
Reflectin 1a device as biosensor for our lalf-LPS interaction in B.subtilis-<br />
<br />
E.coli mixed culture. <em> </em></p><br />
<br />
<p>Disc deneyi1</p><br />
<br />
https://static.igem.org/mediawiki/2011/d/d1/Sss1.png<br />
<br />
<p>There are b.subtilis with LALF in our B1 plate and b.subtilis without LALF in our B2 plate.For working of our LALF protein ,count of e.coli with RFP in B1 is lower than B2.</p><br />
<br />
https://static.igem.org/mediawiki/igem.org/f/fd/Sss2.png<br />
<br />
<p>We spread fresh E.coli which produce RFP and put them onto 100uL Bacillus Subtilis supernatant which include LALF (plate D1) and no LALF protein producing Bacillus Subtilis supernatant(plateD2).As you can see where Bacillus supernatant with LALF exist ,there wasn’t any E.coli with RFP.</p><br />
<br />
https://static.igem.org/mediawiki/2011/8/85/Sss3.png<br />
<br />
<p>We spread fresh E.coli which produce RFP and put them onto 100uL Bacillus Subtilis which include LALF (plateC1 ) and no LALF protein producing Bacillus Subtilis (plateC2).As you can see where Bacillus with LALF exist ,there wasn’t any E.coli with RFP.</p><br />
<br />
<br />
https://static.igem.org/mediawiki/2011/f/f9/IMG_0331.JPG<br />
https://static.igem.org/mediawiki/2011/1/1f/IMG_0335.JPG<br />
https://static.igem.org/mediawiki/2011/a/a6/IMG_0339.JPG<br />
We saw that size of colonies with LALF protein is smaller than size of colonies without LALF protein and also antibiotic didn’t changed the results.<br />
<br />
<p>THE SUICIDE EXPERIMENT OF E. COLI</p><br />
<br />
<p>IPTG was caused of suicide of E.coli.Our promoter is IPTG inducable.So when we added IPTG in the media LALF synthezised.Result of this experiment we can say that E.coli kills itself.</p><br />
<br />
<br />
<br />
<p><strong><em></em><em>For Human Practice; We have performed the activities as follows;</em></strong></p><br />
<br />
<p>1. Providing Sporocide Method for prevention from spores of B.subtilis in further <br />
<br />
B.subtilis synthetic biology project.<em> </em></p><br />
<br />
<p>2. On the other hand, our secondary protein, reflectin, can be used in varying applications.</p><br />
<p>3. Preparing a novel board game `Canvas Time` to inform people from any distinct <br />
<br />
education level about features of our project, laboratory equipments and <br />
<br />
interesting Biobrick of iGEM competition.<br />
.</p><br />
<p>4.Performing open-lab to public meetings such as gifted children studying in <br />
<br />
several schools of Ankara, undergraduate students from Hacettepe University <br />
<br />
Medical School and postgraduate students from Ankara University Medical School<br />
</p><br />
<p>5.Kindergarten synthetic biology trip to teach synthetic biology to five-year-old <br />
<br />
children. <br />
</p><br />
<p><strong><br /><br />
</strong></p><br />
<br />
<br />
<br />
<br />
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http://2011.igem.org/Team:Fatih_Turkey/Results
Team:Fatih Turkey/Results
2011-09-22T03:42:06Z
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<a href="https://2011.igem.org/Team:Fatih_Turkey/Lab_Garage">Lab Garage</a><br />
<ul><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Notebook">Notebook</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Procedures">Procedures</a></li><br />
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<h2 style="font-family: Verdana, Arial, SunSans-Regular, sans-serif;">Project</h2><br />
<h5 style="font-family: Verdana, Arial, SunSans-Regular, sans-serif;">Results</h5> <br />
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<p><em>1. </em><em>technique;</em> in case of applying on surfaces, LALF protein can work like a new sterilization material like gluteraldehyte or alcohol that are commonly used for hospital and lab sterilization.<em> </em></p><br />
<p><em>2. </em><em>Alternative cure for septic shock;</em> after gram negative bacterial infections; LPS, which is the main component of cell wall of gram negative bacteria, can cause deathly conditions that is generally called “septic shock”. In our project, our LALF protein binds this material strictly, thus LALF can be used as an alternative cure method for such condition.<em> </em></p><br />
<p><em>3. </em><em>Possible new structure of antibiotic-like drug; </em>antibiotics commonly effect on production of cell wall of bacteria. This is done by inhibiting the source of production. On the other hand, our LALF protein directly binds the cell wall material and stops the bacteria growth. Thus, a new antibiotic like drug made of LALF may be produced and used in some infections.<em> </em></p><br />
<p><em>4. </em><em>Possibility of producing longtime sterile layers; </em>if any kind of object is produced by covering with LALF, that object can stay sterile for long time because of gram negative bacteria protection of LALF. In case of a possible infection on surface, LALF will stop bacteria growth.<em> </em></p><br />
<p><em>5. </em><em>Alternative cure method for bacterial diseases; </em>diseases like urinary system infection or gastritis are caused by gram negative bacteria.<em> </em>In the course of applying our bacteria that produce LALF in the infectious cite, infection of harmful bacteria can be prevented or stopped. This may suggest an alternative cure for such diseases.<em> </em></p><br />
<p>On the other hand, our secondary protein, reflectin, can be used in varying applications.</p><br />
<p><em>1. </em><em>Camouflage material; </em>this protein allows its host, cephalopod, to hide when cephalopod hunts or to defend itself from its hunters. In the course of development of reflectin protein, a new camouflage material may be produced for humans.<em> </em></p><br />
<p><em>2. </em><em>New material of dye for varying applications; </em>Dye that changes its color can be used almost every colorful application. From fireworks to wall paints, from windows of buildings to road signs, this ability can be very useful.<em></em></p><br />
<p><em>3. </em><em>Possibility of producing specialized light colors; </em>in some circumstances, a unique light color may be necessary in order to gain maximum output like in greenhouses or dark rooms in photography industry. Reflectin reflects the light by changing its wavelength; therefore the light that is reflected has only one color. This ability can be applied in such application that mentioned above.<em></em></p><br />
<p><strong><br /><br />
</strong></p><br />
<br />
<p>COLLABORATION </p><br />
<br />
<p>With friendly communications teams can take some useful advises and learn some <br />
<br />
important points. Also with helping to other groups friendly feelings increase <br />
<br />
and the team’s motivation increases. During our lab working we were always <br />
<br />
keeping touch with other teams. We were helping each other and making some <br />
<br />
activities together. </p><br />
<br />
<br />
<p>We helped to Bilkent_UNAM_Turkey team when they needed our part 1001 and they <br />
<br />
helped to us with giving LB Agar. Also the Team Michigan asked helping to us <br />
<br />
about board game. We designed the Canvas Town Game and the Team Michigan wanted <br />
<br />
some points about preparing and designing. And we were being glad to help them.<br />
Association of Synthetic Biology – METU-Ankara Team invited our team to <br />
<br />
Synthetic Biology Turkey Meetings 1.0 of Igem Turkey Teams. This meeting was <br />
<br />
really funny and informative. </p><br />
<br />
<br />
<p>We are planning a little tournament of Canvas Town Game in the Asia Religion <br />
<br />
Jamboree. For this organization we help from Religion Asia Organization <br />
<br />
Committee and they helped us very quickly and politely.</p><br />
<br />
<br />
<p>For the tournament four teams will determine with drawing. 2 teams will play <br />
<br />
together and the other 2 teams will play together. Just 6 persons can play our <br />
<br />
game so every team should play with their 3 members. Then the winner teams will <br />
<br />
play together in the end of this game 3 finalist member of the last winner team <br />
<br />
will be presented.</p><br />
<br />
<br />
<p>With these activities we were really enjoyed and informed.</p><br />
<br />
<br />
<br />
<p><div><br />
<br />
<div><br />
<div class="meta-left-full"> <br />
<p><strong><em></em><em>For our project lab executions;</em></strong></p><br />
<br />
<p> 1. LALF based anti-LPS factor system has been worked expectedly<em> </em></p><br />
<p><em>2.we have constructed and submitted 12 biobrick. Three devices working in two for <br />
<br />
B.subtilis one for E.coli work expectedly.<br />
<em> </em></p><br />
<p><em>3. Our B.subtilis specific promoter with SacB- extracellur signal- has been seen <br />
<br />
working well.<em> </em></p><br />
<br />
<p><em>4. We have synthesized Reflectin 1a protein in E.coli; however, because we did not <br />
<br />
have enough time to purify it to visualize via aid-eye, we could not use <br />
<br />
Reflectin 1a device as biosensor for our lalf-LPS interaction in B.subtilis-<br />
<br />
E.coli mixed culture. <em> </em></p><br />
<br />
<br />
<br />
<p><strong><em></em><em>For Human Practice; We have performed the activities as follows;</em></strong></p><br />
<br />
<p>1. Providing Sporocide Method for prevention from spores of B.subtilis in further <br />
<br />
B.subtilis synthetic biology project.<em> </em></p><br />
<br />
<p>2. On the other hand, our secondary protein, reflectin, can be used in varying applications.</p><br />
<p>3. Preparing a novel board game `Canvas Time` to inform people from any distinct <br />
<br />
education level about features of our project, laboratory equipments and <br />
<br />
interesting Biobrick of iGEM competition.<br />
.</p><br />
<p>4.Performing open-lab to public meetings such as gifted children studying in <br />
<br />
several schools of Ankara, undergraduate students from Hacettepe University <br />
<br />
Medical School and postgraduate students from Ankara University Medical School<br />
</p><br />
<p>5.Kindergarten synthetic biology trip to teach synthetic biology to five-year-old <br />
<br />
children. <br />
</p><br />
<p><strong><br /><br />
</strong></p><br />
<br />
<br />
<br />
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http://2011.igem.org/Team:Fatih_Turkey/Results
Team:Fatih Turkey/Results
2011-09-22T03:40:41Z
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<h2 style="font-family: Verdana, Arial, SunSans-Regular, sans-serif;">Project</h2><br />
<h5 style="font-family: Verdana, Arial, SunSans-Regular, sans-serif;">Results</h5> <br />
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<p><em>1. </em><em>technique;</em> in case of applying on surfaces, LALF protein can work like a new sterilization material like gluteraldehyte or alcohol that are commonly used for hospital and lab sterilization.<em> </em></p><br />
<p><em>2. </em><em>Alternative cure for septic shock;</em> after gram negative bacterial infections; LPS, which is the main component of cell wall of gram negative bacteria, can cause deathly conditions that is generally called “septic shock”. In our project, our LALF protein binds this material strictly, thus LALF can be used as an alternative cure method for such condition.<em> </em></p><br />
<p><em>3. </em><em>Possible new structure of antibiotic-like drug; </em>antibiotics commonly effect on production of cell wall of bacteria. This is done by inhibiting the source of production. On the other hand, our LALF protein directly binds the cell wall material and stops the bacteria growth. Thus, a new antibiotic like drug made of LALF may be produced and used in some infections.<em> </em></p><br />
<p><em>4. </em><em>Possibility of producing longtime sterile layers; </em>if any kind of object is produced by covering with LALF, that object can stay sterile for long time because of gram negative bacteria protection of LALF. In case of a possible infection on surface, LALF will stop bacteria growth.<em> </em></p><br />
<p><em>5. </em><em>Alternative cure method for bacterial diseases; </em>diseases like urinary system infection or gastritis are caused by gram negative bacteria.<em> </em>In the course of applying our bacteria that produce LALF in the infectious cite, infection of harmful bacteria can be prevented or stopped. This may suggest an alternative cure for such diseases.<em> </em></p><br />
<p>On the other hand, our secondary protein, reflectin, can be used in varying applications.</p><br />
<p><em>1. </em><em>Camouflage material; </em>this protein allows its host, cephalopod, to hide when cephalopod hunts or to defend itself from its hunters. In the course of development of reflectin protein, a new camouflage material may be produced for humans.<em> </em></p><br />
<p><em>2. </em><em>New material of dye for varying applications; </em>Dye that changes its color can be used almost every colorful application. From fireworks to wall paints, from windows of buildings to road signs, this ability can be very useful.<em></em></p><br />
<p><em>3. </em><em>Possibility of producing specialized light colors; </em>in some circumstances, a unique light color may be necessary in order to gain maximum output like in greenhouses or dark rooms in photography industry. Reflectin reflects the light by changing its wavelength; therefore the light that is reflected has only one color. This ability can be applied in such application that mentioned above.<em></em></p><br />
<p><strong><br /><br />
</strong></p><br />
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<p><strong><em></em><em>For our project lab executions;</em></strong></p><br />
<br />
<p> 1. LALF based anti-LPS factor system has been worked expectedly<em> </em></p><br />
<p><em>2.we have constructed and submitted 12 biobrick. Three devices working in two for <br />
<br />
B.subtilis one for E.coli work expectedly.<br />
<em> </em></p><br />
<p><em>3. Our B.subtilis specific promoter with SacB- extracellur signal- has been seen <br />
<br />
working well.<em> </em></p><br />
<br />
<p><em>4. We have synthesized Reflectin 1a protein in E.coli; however, because we did not <br />
<br />
have enough time to purify it to visualize via aid-eye, we could not use <br />
<br />
Reflectin 1a device as biosensor for our lalf-LPS interaction in B.subtilis-<br />
<br />
E.coli mixed culture. <em> </em></p><br />
<br />
<br />
<br />
<p><strong><em></em><em>For Human Practice; We have performed the activities as follows;</em></strong></p><br />
<br />
<p>1. Providing Sporocide Method for prevention from spores of B.subtilis in further <br />
<br />
B.subtilis synthetic biology project.<em> </em></p><br />
<br />
<p>2. On the other hand, our secondary protein, reflectin, can be used in varying applications.</p><br />
<p>3. Preparing a novel board game `Canvas Time` to inform people from any distinct <br />
<br />
education level about features of our project, laboratory equipments and <br />
<br />
interesting Biobrick of iGEM competition.<br />
.</p><br />
<p>4.Performing open-lab to public meetings such as gifted children studying in <br />
<br />
several schools of Ankara, undergraduate students from Hacettepe University <br />
<br />
Medical School and postgraduate students from Ankara University Medical School<br />
</p><br />
<p>5.Kindergarten synthetic biology trip to teach synthetic biology to five-year-old <br />
<br />
children. <br />
</p><br />
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Metugen
http://2011.igem.org/Team:Fatih_Turkey/Results
Team:Fatih Turkey/Results
2011-09-22T03:39:52Z
<p>Metugen: </p>
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<h2 style="font-family: Verdana, Arial, SunSans-Regular, sans-serif;">Project</h2><br />
<h5 style="font-family: Verdana, Arial, SunSans-Regular, sans-serif;">Results</h5> <br />
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<p><em>1. </em><em>technique;</em> in case of applying on surfaces, LALF protein can work like a new sterilization material like gluteraldehyte or alcohol that are commonly used for hospital and lab sterilization.<em> </em></p><br />
<p><em>2. </em><em>Alternative cure for septic shock;</em> after gram negative bacterial infections; LPS, which is the main component of cell wall of gram negative bacteria, can cause deathly conditions that is generally called “septic shock”. In our project, our LALF protein binds this material strictly, thus LALF can be used as an alternative cure method for such condition.<em> </em></p><br />
<p><em>3. </em><em>Possible new structure of antibiotic-like drug; </em>antibiotics commonly effect on production of cell wall of bacteria. This is done by inhibiting the source of production. On the other hand, our LALF protein directly binds the cell wall material and stops the bacteria growth. Thus, a new antibiotic like drug made of LALF may be produced and used in some infections.<em> </em></p><br />
<p><em>4. </em><em>Possibility of producing longtime sterile layers; </em>if any kind of object is produced by covering with LALF, that object can stay sterile for long time because of gram negative bacteria protection of LALF. In case of a possible infection on surface, LALF will stop bacteria growth.<em> </em></p><br />
<p><em>5. </em><em>Alternative cure method for bacterial diseases; </em>diseases like urinary system infection or gastritis are caused by gram negative bacteria.<em> </em>In the course of applying our bacteria that produce LALF in the infectious cite, infection of harmful bacteria can be prevented or stopped. This may suggest an alternative cure for such diseases.<em> </em></p><br />
<p>On the other hand, our secondary protein, reflectin, can be used in varying applications.</p><br />
<p><em>1. </em><em>Camouflage material; </em>this protein allows its host, cephalopod, to hide when cephalopod hunts or to defend itself from its hunters. In the course of development of reflectin protein, a new camouflage material may be produced for humans.<em> </em></p><br />
<p><em>2. </em><em>New material of dye for varying applications; </em>Dye that changes its color can be used almost every colorful application. From fireworks to wall paints, from windows of buildings to road signs, this ability can be very useful.<em></em></p><br />
<p><em>3. </em><em>Possibility of producing specialized light colors; </em>in some circumstances, a unique light color may be necessary in order to gain maximum output like in greenhouses or dark rooms in photography industry. Reflectin reflects the light by changing its wavelength; therefore the light that is reflected has only one color. This ability can be applied in such application that mentioned above.<em></em></p><br />
<p><strong><br /><br />
</strong></p><br />
<br />
<br />
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<p><em></em><em>For our project lab executions;</em></p><br />
<br />
<p> 1. LALF based anti-LPS factor system has been worked expectedly<em> </em></p><br />
<p><em>2.we have constructed and submitted 12 biobrick. Three devices working in two for <br />
<br />
B.subtilis one for E.coli work expectedly.<br />
<em> </em></p><br />
<p><em>3. Our B.subtilis specific promoter with SacB- extracellur signal- has been seen <br />
<br />
working well.<em> </em></p><br />
<br />
<p><em>4. We have synthesized Reflectin 1a protein in E.coli; however, because we did not <br />
<br />
have enough time to purify it to visualize via aid-eye, we could not use <br />
<br />
Reflectin 1a device as biosensor for our lalf-LPS interaction in B.subtilis-<br />
<br />
E.coli mixed culture. <em> </em></p><br />
<br />
<br />
<br />
<p><em></em><em>For Human Practice; We have performed the activities as follows;</em></p><br />
<br />
<p>1. Providing Sporocide Method for prevention from spores of B.subtilis in further <br />
<br />
B.subtilis synthetic biology project.<em> </em></p><br />
<br />
<p>2. On the other hand, our secondary protein, reflectin, can be used in varying applications.</p><br />
<p>3. Preparing a novel board game `Canvas Time` to inform people from any distinct <br />
<br />
education level about features of our project, laboratory equipments and <br />
<br />
interesting Biobrick of iGEM competition.<br />
.</p><br />
<p>4.Performing open-lab to public meetings such as gifted children studying in <br />
<br />
several schools of Ankara, undergraduate students from Hacettepe University <br />
<br />
Medical School and postgraduate students from Ankara University Medical School<br />
</p><br />
<p>5.Kindergarten synthetic biology trip to teach synthetic biology to five-year-old <br />
<br />
children. <br />
</p><br />
<p><strong><br /><br />
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Metugen
http://2011.igem.org/Team:Fatih_Turkey/Results
Team:Fatih Turkey/Results
2011-09-22T03:37:05Z
<p>Metugen: </p>
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<h2 style="font-family: Verdana, Arial, SunSans-Regular, sans-serif;">Project</h2><br />
<h5 style="font-family: Verdana, Arial, SunSans-Regular, sans-serif;">Results</h5> <br />
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<p><em>1. </em><em>technique;</em> in case of applying on surfaces, LALF protein can work like a new sterilization material like gluteraldehyte or alcohol that are commonly used for hospital and lab sterilization.<em> </em></p><br />
<p><em>2. </em><em>Alternative cure for septic shock;</em> after gram negative bacterial infections; LPS, which is the main component of cell wall of gram negative bacteria, can cause deathly conditions that is generally called “septic shock”. In our project, our LALF protein binds this material strictly, thus LALF can be used as an alternative cure method for such condition.<em> </em></p><br />
<p><em>3. </em><em>Possible new structure of antibiotic-like drug; </em>antibiotics commonly effect on production of cell wall of bacteria. This is done by inhibiting the source of production. On the other hand, our LALF protein directly binds the cell wall material and stops the bacteria growth. Thus, a new antibiotic like drug made of LALF may be produced and used in some infections.<em> </em></p><br />
<p><em>4. </em><em>Possibility of producing longtime sterile layers; </em>if any kind of object is produced by covering with LALF, that object can stay sterile for long time because of gram negative bacteria protection of LALF. In case of a possible infection on surface, LALF will stop bacteria growth.<em> </em></p><br />
<p><em>5. </em><em>Alternative cure method for bacterial diseases; </em>diseases like urinary system infection or gastritis are caused by gram negative bacteria.<em> </em>In the course of applying our bacteria that produce LALF in the infectious cite, infection of harmful bacteria can be prevented or stopped. This may suggest an alternative cure for such diseases.<em> </em></p><br />
<p>On the other hand, our secondary protein, reflectin, can be used in varying applications.</p><br />
<p><em>1. </em><em>Camouflage material; </em>this protein allows its host, cephalopod, to hide when cephalopod hunts or to defend itself from its hunters. In the course of development of reflectin protein, a new camouflage material may be produced for humans.<em> </em></p><br />
<p><em>2. </em><em>New material of dye for varying applications; </em>Dye that changes its color can be used almost every colorful application. From fireworks to wall paints, from windows of buildings to road signs, this ability can be very useful.<em></em></p><br />
<p><em>3. </em><em>Possibility of producing specialized light colors; </em>in some circumstances, a unique light color may be necessary in order to gain maximum output like in greenhouses or dark rooms in photography industry. Reflectin reflects the light by changing its wavelength; therefore the light that is reflected has only one color. This ability can be applied in such application that mentioned above.<em></em></p><br />
<p><strong><br /><br />
</strong></p><br />
<br />
<br />
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<p><em></em><em>For our project lab executions;</em></p><br />
<br />
<p> 1. LALF based anti-LPS factor system has been worked expectedly<em> </em></p><br />
<p><em>2.we have constructed and submitted 12 biobrick. Three devices working in two for <br />
<br />
B.subtilis one for E.coli work expectedly.<br />
<em> </em></p><br />
<p><em>3. Our B.subtilis specific promoter with SacB- extracellur signal- has been seen <br />
<br />
working well.<em> </em></p><br />
<br />
<p><em>4. We have synthesized Reflectin 1a protein in E.coli; however, because we did not <br />
<br />
have enough time to purify it to visualize via aid-eye, we could not use <br />
<br />
Reflectin 1a device as biosensor for our lalf-LPS interaction in B.subtilis-<br />
<br />
E.coli mixed culture. <em> </em></p><br />
<br />
<br />
<br />
<p><em></em><em>For Human Practice; We have performed the activities as follows;</em></p><br />
<br />
<p>1. Providing Sporocide Method for prevention from spores of B.subtilis in further <br />
<br />
B.subtilis synthetic biology project.<em> </em></p><br />
<br />
<p>2. On the other hand, our secondary protein, reflectin, can be used in varying applications.</p><br />
<p><em>3. Preparing a novel board game `Canvas Time` to inform people from any distinct <br />
<br />
education level about features of our project, laboratory equipments and <br />
<br />
interesting Biobrick of iGEM competition.<br />
.<em> </em></p><br />
<p><em>2. </em><em>New material of dye for varying applications; </em>Dye that changes its color can be used almost every colorful application. From fireworks to wall paints, from windows of buildings to road signs, this ability can be very useful.<em></em></p><br />
<p><em>3. </em><em>Possibility of producing specialized light colors; </em>in some circumstances, a unique light color may be necessary in order to gain maximum output like in greenhouses or dark rooms in photography industry. Reflectin reflects the light by changing its wavelength; therefore the light that is reflected has only one color. This ability can be applied in such application that mentioned above.<em></em></p><br />
<p><strong><br /><br />
</strong></p><br />
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Metugen
http://2011.igem.org/Team:Fatih_Turkey/Results
Team:Fatih Turkey/Results
2011-09-22T03:33:37Z
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<h2 style="font-family: Verdana, Arial, SunSans-Regular, sans-serif;">Project</h2><br />
<h5 style="font-family: Verdana, Arial, SunSans-Regular, sans-serif;">Results</h5> <br />
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<p><em>1. </em><em>technique;</em> in case of applying on surfaces, LALF protein can work like a new sterilization material like gluteraldehyte or alcohol that are commonly used for hospital and lab sterilization.<em> </em></p><br />
<p><em>2. </em><em>Alternative cure for septic shock;</em> after gram negative bacterial infections; LPS, which is the main component of cell wall of gram negative bacteria, can cause deathly conditions that is generally called “septic shock”. In our project, our LALF protein binds this material strictly, thus LALF can be used as an alternative cure method for such condition.<em> </em></p><br />
<p><em>3. </em><em>Possible new structure of antibiotic-like drug; </em>antibiotics commonly effect on production of cell wall of bacteria. This is done by inhibiting the source of production. On the other hand, our LALF protein directly binds the cell wall material and stops the bacteria growth. Thus, a new antibiotic like drug made of LALF may be produced and used in some infections.<em> </em></p><br />
<p><em>4. </em><em>Possibility of producing longtime sterile layers; </em>if any kind of object is produced by covering with LALF, that object can stay sterile for long time because of gram negative bacteria protection of LALF. In case of a possible infection on surface, LALF will stop bacteria growth.<em> </em></p><br />
<p><em>5. </em><em>Alternative cure method for bacterial diseases; </em>diseases like urinary system infection or gastritis are caused by gram negative bacteria.<em> </em>In the course of applying our bacteria that produce LALF in the infectious cite, infection of harmful bacteria can be prevented or stopped. This may suggest an alternative cure for such diseases.<em> </em></p><br />
<p>On the other hand, our secondary protein, reflectin, can be used in varying applications.</p><br />
<p><em>1. </em><em>Camouflage material; </em>this protein allows its host, cephalopod, to hide when cephalopod hunts or to defend itself from its hunters. In the course of development of reflectin protein, a new camouflage material may be produced for humans.<em> </em></p><br />
<p><em>2. </em><em>New material of dye for varying applications; </em>Dye that changes its color can be used almost every colorful application. From fireworks to wall paints, from windows of buildings to road signs, this ability can be very useful.<em></em></p><br />
<p><em>3. </em><em>Possibility of producing specialized light colors; </em>in some circumstances, a unique light color may be necessary in order to gain maximum output like in greenhouses or dark rooms in photography industry. Reflectin reflects the light by changing its wavelength; therefore the light that is reflected has only one color. This ability can be applied in such application that mentioned above.<em></em></p><br />
<p><strong><br /><br />
</strong></p><br />
<br />
<br />
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<p><em></em><em>For our project lab executions;</em> 1. LALF based anti-LPS factor system has been worked expectedly<em> </em></p><br />
<p><em>2. </em><em>Alternative cure for septic shock;</em> after gram negative bacterial infections; LPS, which is the main component of cell wall of gram negative bacteria, can cause deathly conditions that is generally called “septic shock”. In our project, our LALF protein binds this material strictly, thus LALF can be used as an alternative cure method for such condition.<em> </em></p><br />
<p><em>3. </em><em>Possible new structure of antibiotic-like drug; </em>antibiotics commonly effect on production of cell wall of bacteria. This is done by inhibiting the source of production. On the other hand, our LALF protein directly binds the cell wall material and stops the bacteria growth. Thus, a new antibiotic like drug made of LALF may be produced and used in some infections.<em> </em></p><br />
<p><em>4. </em><em>Possibility of producing longtime sterile layers; </em>if any kind of object is produced by covering with LALF, that object can stay sterile for long time because of gram negative bacteria protection of LALF. In case of a possible infection on surface, LALF will stop bacteria growth.<em> </em></p><br />
<p><em>5. </em><em>Alternative cure method for bacterial diseases; </em>diseases like urinary system infection or gastritis are caused by gram negative bacteria.<em> </em>In the course of applying our bacteria that produce LALF in the infectious cite, infection of harmful bacteria can be prevented or stopped. This may suggest an alternative cure for such diseases.<em> </em></p><br />
<p>On the other hand, our secondary protein, reflectin, can be used in varying applications.</p><br />
<p><em>1. </em><em>Camouflage material; </em>this protein allows its host, cephalopod, to hide when cephalopod hunts or to defend itself from its hunters. In the course of development of reflectin protein, a new camouflage material may be produced for humans.<em> </em></p><br />
<p><em>2. </em><em>New material of dye for varying applications; </em>Dye that changes its color can be used almost every colorful application. From fireworks to wall paints, from windows of buildings to road signs, this ability can be very useful.<em></em></p><br />
<p><em>3. </em><em>Possibility of producing specialized light colors; </em>in some circumstances, a unique light color may be necessary in order to gain maximum output like in greenhouses or dark rooms in photography industry. Reflectin reflects the light by changing its wavelength; therefore the light that is reflected has only one color. This ability can be applied in such application that mentioned above.<em></em></p><br />
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http://2011.igem.org/Team:Fatih_Turkey/Results
Team:Fatih Turkey/Results
2011-09-22T03:32:34Z
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<h2 style="font-family: Verdana, Arial, SunSans-Regular, sans-serif;">Project</h2><br />
<h5 style="font-family: Verdana, Arial, SunSans-Regular, sans-serif;">Results</h5> <br />
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<p><em>1. </em><em>technique;</em> in case of applying on surfaces, LALF protein can work like a new sterilization material like gluteraldehyte or alcohol that are commonly used for hospital and lab sterilization.<em> </em></p><br />
<p><em>2. </em><em>Alternative cure for septic shock;</em> after gram negative bacterial infections; LPS, which is the main component of cell wall of gram negative bacteria, can cause deathly conditions that is generally called “septic shock”. In our project, our LALF protein binds this material strictly, thus LALF can be used as an alternative cure method for such condition.<em> </em></p><br />
<p><em>3. </em><em>Possible new structure of antibiotic-like drug; </em>antibiotics commonly effect on production of cell wall of bacteria. This is done by inhibiting the source of production. On the other hand, our LALF protein directly binds the cell wall material and stops the bacteria growth. Thus, a new antibiotic like drug made of LALF may be produced and used in some infections.<em> </em></p><br />
<p><em>4. </em><em>Possibility of producing longtime sterile layers; </em>if any kind of object is produced by covering with LALF, that object can stay sterile for long time because of gram negative bacteria protection of LALF. In case of a possible infection on surface, LALF will stop bacteria growth.<em> </em></p><br />
<p><em>5. </em><em>Alternative cure method for bacterial diseases; </em>diseases like urinary system infection or gastritis are caused by gram negative bacteria.<em> </em>In the course of applying our bacteria that produce LALF in the infectious cite, infection of harmful bacteria can be prevented or stopped. This may suggest an alternative cure for such diseases.<em> </em></p><br />
<p>On the other hand, our secondary protein, reflectin, can be used in varying applications.</p><br />
<p><em>1. </em><em>Camouflage material; </em>this protein allows its host, cephalopod, to hide when cephalopod hunts or to defend itself from its hunters. In the course of development of reflectin protein, a new camouflage material may be produced for humans.<em> </em></p><br />
<p><em>2. </em><em>New material of dye for varying applications; </em>Dye that changes its color can be used almost every colorful application. From fireworks to wall paints, from windows of buildings to road signs, this ability can be very useful.<em></em></p><br />
<p><em>3. </em><em>Possibility of producing specialized light colors; </em>in some circumstances, a unique light color may be necessary in order to gain maximum output like in greenhouses or dark rooms in photography industry. Reflectin reflects the light by changing its wavelength; therefore the light that is reflected has only one color. This ability can be applied in such application that mentioned above.<em></em></p><br />
<p><strong><br /><br />
</strong></p><br />
<br />
<br />
<p><div><br />
<br />
<div><br />
<div class="meta-left-full"> <br />
<p><em>1. </em><em>technique;</em> in case of applying on surfaces, LALF protein can work like a new sterilization material like gluteraldehyte or alcohol that are commonly used for hospital and lab sterilization.<em> </em></p><br />
<p><em>2. </em><em>Alternative cure for septic shock;</em> after gram negative bacterial infections; LPS, which is the main component of cell wall of gram negative bacteria, can cause deathly conditions that is generally called “septic shock”. In our project, our LALF protein binds this material strictly, thus LALF can be used as an alternative cure method for such condition.<em> </em></p><br />
<p><em>3. </em><em>Possible new structure of antibiotic-like drug; </em>antibiotics commonly effect on production of cell wall of bacteria. This is done by inhibiting the source of production. On the other hand, our LALF protein directly binds the cell wall material and stops the bacteria growth. Thus, a new antibiotic like drug made of LALF may be produced and used in some infections.<em> </em></p><br />
<p><em>4. </em><em>Possibility of producing longtime sterile layers; </em>if any kind of object is produced by covering with LALF, that object can stay sterile for long time because of gram negative bacteria protection of LALF. In case of a possible infection on surface, LALF will stop bacteria growth.<em> </em></p><br />
<p><em>5. </em><em>Alternative cure method for bacterial diseases; </em>diseases like urinary system infection or gastritis are caused by gram negative bacteria.<em> </em>In the course of applying our bacteria that produce LALF in the infectious cite, infection of harmful bacteria can be prevented or stopped. This may suggest an alternative cure for such diseases.<em> </em></p><br />
<p>On the other hand, our secondary protein, reflectin, can be used in varying applications.</p><br />
<p><em>1. </em><em>Camouflage material; </em>this protein allows its host, cephalopod, to hide when cephalopod hunts or to defend itself from its hunters. In the course of development of reflectin protein, a new camouflage material may be produced for humans.<em> </em></p><br />
<p><em>2. </em><em>New material of dye for varying applications; </em>Dye that changes its color can be used almost every colorful application. From fireworks to wall paints, from windows of buildings to road signs, this ability can be very useful.<em></em></p><br />
<p><em>3. </em><em>Possibility of producing specialized light colors; </em>in some circumstances, a unique light color may be necessary in order to gain maximum output like in greenhouses or dark rooms in photography industry. Reflectin reflects the light by changing its wavelength; therefore the light that is reflected has only one color. This ability can be applied in such application that mentioned above.<em></em></p><br />
<p><strong><br /><br />
</strong></p><br />
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Metugen
http://2011.igem.org/Team:Fatih_Turkey/Results
Team:Fatih Turkey/Results
2011-09-22T03:31:47Z
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<h2 style="font-family: Verdana, Arial, SunSans-Regular, sans-serif;">Project</h2><br />
<h5 style="font-family: Verdana, Arial, SunSans-Regular, sans-serif;">Results</h5> <br />
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<p><em>1. </em><em>technique;</em> in case of applying on surfaces, LALF protein can work like a new sterilization material like gluteraldehyte or alcohol that are commonly used for hospital and lab sterilization.<em> </em></p><br />
<p><em>2. </em><em>Alternative cure for septic shock;</em> after gram negative bacterial infections; LPS, which is the main component of cell wall of gram negative bacteria, can cause deathly conditions that is generally called “septic shock”. In our project, our LALF protein binds this material strictly, thus LALF can be used as an alternative cure method for such condition.<em> </em></p><br />
<p><em>3. </em><em>Possible new structure of antibiotic-like drug; </em>antibiotics commonly effect on production of cell wall of bacteria. This is done by inhibiting the source of production. On the other hand, our LALF protein directly binds the cell wall material and stops the bacteria growth. Thus, a new antibiotic like drug made of LALF may be produced and used in some infections.<em> </em></p><br />
<p><em>4. </em><em>Possibility of producing longtime sterile layers; </em>if any kind of object is produced by covering with LALF, that object can stay sterile for long time because of gram negative bacteria protection of LALF. In case of a possible infection on surface, LALF will stop bacteria growth.<em> </em></p><br />
<p><em>5. </em><em>Alternative cure method for bacterial diseases; </em>diseases like urinary system infection or gastritis are caused by gram negative bacteria.<em> </em>In the course of applying our bacteria that produce LALF in the infectious cite, infection of harmful bacteria can be prevented or stopped. This may suggest an alternative cure for such diseases.<em> </em></p><br />
<p>On the other hand, our secondary protein, reflectin, can be used in varying applications.</p><br />
<p><em>1. </em><em>Camouflage material; </em>this protein allows its host, cephalopod, to hide when cephalopod hunts or to defend itself from its hunters. In the course of development of reflectin protein, a new camouflage material may be produced for humans.<em> </em></p><br />
<p><em>2. </em><em>New material of dye for varying applications; </em>Dye that changes its color can be used almost every colorful application. From fireworks to wall paints, from windows of buildings to road signs, this ability can be very useful.<em></em></p><br />
<p><em>3. </em><em>Possibility of producing specialized light colors; </em>in some circumstances, a unique light color may be necessary in order to gain maximum output like in greenhouses or dark rooms in photography industry. Reflectin reflects the light by changing its wavelength; therefore the light that is reflected has only one color. This ability can be applied in such application that mentioned above.<em></em></p><br />
<p><strong><br /><br />
</strong></p><br />
<br />
<br />
<p><div><br />
For our project lab executions;</div><br />
<br />
<div>LALF based anti-LPS factor system has been worked expectedly.</div><br />
<br />
<div>We have constructed and submitted 12 biobrick. Three devices working in two for <br />
<br />
B.subtilis one for E.coli work expectedly.</div><br />
<br />
<div>Our B.subtilis specific promoter with SacB- extracellur signal- has been seen working well.</div><br />
<br />
<div>We have synthesized Reflectin 1a protein in E.coli; however, because we did not have enough time to purify it to visualize via aid-eye, we could not use Reflectin 1a device as biosensor for our lalf-LPS interaction in B.subtilis-<br />
E.coli mixed culture. </div><br />
<br />
<br />
<br />
<br />
For Human Practice; We have performed the activities as follows;<br />
<br />
Providing Sporocide Method for prevention from spores of B.subtilis in further <br />
<br />
B.subtilis synthetic biology project.<br />
<br />
Preparing a novel board game `Canvas Time` to inform people from any distinct <br />
<br />
education level about features of our project, laboratory equipments and <br />
<br />
interesting Biobrick of iGEM competition.<br />
<br />
Meeting other iGEM teams from Turkey in SYNTHETIC BIOLOGY TURKEY MEETING.<br />
<br />
Stroving for the innovation and presentation of synthetic biology to people. As <br />
<br />
a medical school, our hospital has its own quarterly magazine, “Yasama Sanati” <br />
<br />
(in English: The Art of Living). <br />
<br />
Preparing a journal which is named “Canvas Times” and it gives you the actual <br />
<br />
news of some time to fun and talk with people about our project, synthetic <br />
<br />
biology and iGEM... Our all of activities and human practices are existed on our <br />
<br />
journal such as “iGEM Picnic” and “International Medical Students Conference. <br />
<br />
Performinf open-lab to public meetings such as gifted children studying in <br />
<br />
several schools of Ankara, undergraduate students from Hacettepe University <br />
<br />
Medical School and postgraduate students from Ankara University Medical School<br />
<br />
Kindergarten synthetic biology trip to teach synthetic biology to five-year-old <br />
<br />
children. <br />
<br />
COLLABORATION <br />
<br />
With friendly communications teams can take some useful advises and learn some <br />
<br />
important points. Also with helping to other groups friendly feelings increase <br />
<br />
and the team’s motivation increases. During our lab working we were always <br />
<br />
keeping touch with other teams. We were helping each other and making some <br />
<br />
activities together. <br />
<br />
We helped to Bilkent_UNAM_Turkey team when they needed our part 1001 and they <br />
<br />
helped to us with giving LB Agar. Also the Team Michigan asked helping to us <br />
<br />
about board game. We designed the Canvas Town Game and the Team Michigan wanted <br />
<br />
some points about preparing and designing. And we were being glad to help them.<br />
Association of Synthetic Biology – METU-Ankara Team invited our team to <br />
<br />
Synthetic Biology Turkey Meetings 1.0 of Igem Turkey Teams. This meeting was <br />
<br />
really funny and informative. <br />
<br />
We are planning a little tournament of Canvas Town Game in the Asia Religion <br />
<br />
Jamboree. For this organization we help from Religion Asia Organization <br />
<br />
Committee and they helped us very quickly and politely.<br />
<br />
For the tournament four teams will determine with drawing. 2 teams will play <br />
<br />
together and the other 2 teams will play together. Just 6 persons can play our <br />
<br />
game so every team should play with their 3 members. Then the winner teams will <br />
<br />
play together in the end of this game 3 finalist member of the last winner team <br />
<br />
will be presented.<br />
<br />
With these activities we were really enjoyed and informed.</p><br />
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Metugen
http://2011.igem.org/Team:Fatih_Turkey/Results
Team:Fatih Turkey/Results
2011-09-22T03:30:52Z
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<h2 style="font-family: Verdana, Arial, SunSans-Regular, sans-serif;">Project</h2><br />
<h5 style="font-family: Verdana, Arial, SunSans-Regular, sans-serif;">Results</h5> <br />
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<p><em>1. </em><em>technique;</em> in case of applying on surfaces, LALF protein can work like a new sterilization material like gluteraldehyte or alcohol that are commonly used for hospital and lab sterilization.<em> </em></p><br />
<p><em>2. </em><em>Alternative cure for septic shock;</em> after gram negative bacterial infections; LPS, which is the main component of cell wall of gram negative bacteria, can cause deathly conditions that is generally called “septic shock”. In our project, our LALF protein binds this material strictly, thus LALF can be used as an alternative cure method for such condition.<em> </em></p><br />
<p><em>3. </em><em>Possible new structure of antibiotic-like drug; </em>antibiotics commonly effect on production of cell wall of bacteria. This is done by inhibiting the source of production. On the other hand, our LALF protein directly binds the cell wall material and stops the bacteria growth. Thus, a new antibiotic like drug made of LALF may be produced and used in some infections.<em> </em></p><br />
<p><em>4. </em><em>Possibility of producing longtime sterile layers; </em>if any kind of object is produced by covering with LALF, that object can stay sterile for long time because of gram negative bacteria protection of LALF. In case of a possible infection on surface, LALF will stop bacteria growth.<em> </em></p><br />
<p><em>5. </em><em>Alternative cure method for bacterial diseases; </em>diseases like urinary system infection or gastritis are caused by gram negative bacteria.<em> </em>In the course of applying our bacteria that produce LALF in the infectious cite, infection of harmful bacteria can be prevented or stopped. This may suggest an alternative cure for such diseases.<em> </em></p><br />
<p>On the other hand, our secondary protein, reflectin, can be used in varying applications.</p><br />
<p><em>1. </em><em>Camouflage material; </em>this protein allows its host, cephalopod, to hide when cephalopod hunts or to defend itself from its hunters. In the course of development of reflectin protein, a new camouflage material may be produced for humans.<em> </em></p><br />
<p><em>2. </em><em>New material of dye for varying applications; </em>Dye that changes its color can be used almost every colorful application. From fireworks to wall paints, from windows of buildings to road signs, this ability can be very useful.<em></em></p><br />
<p><em>3. </em><em>Possibility of producing specialized light colors; </em>in some circumstances, a unique light color may be necessary in order to gain maximum output like in greenhouses or dark rooms in photography industry. Reflectin reflects the light by changing its wavelength; therefore the light that is reflected has only one color. This ability can be applied in such application that mentioned above.<em></em></p><br />
<p><strong><br /><br />
</strong></p><br />
<br />
<br />
<p><div><br />
For our project lab executions;</div><br />
<br />
<div>LALF based anti-LPS factor system has been worked expectedly.</div><br />
<br />
<br />
we have constructed and submitted 12 biobrick. Three devices working in two for <br />
<br />
B.subtilis one for E.coli work expectedly.<br />
<br />
Our B.subtilis specific promoter with SacB- extracellur signal- has been seen <br />
<br />
working well.<br />
<br />
<br />
We have synthesized Reflectin 1a protein in E.coli; however, because we did not <br />
<br />
have enough time to purify it to visualize via aid-eye, we could not use <br />
<br />
Reflectin 1a device as biosensor for our lalf-LPS interaction in B.subtilis-<br />
<br />
E.coli mixed culture. <br />
<br />
<br />
<br />
<br />
For Human Practice; We have performed the activities as follows;<br />
<br />
Providing Sporocide Method for prevention from spores of B.subtilis in further <br />
<br />
B.subtilis synthetic biology project.<br />
<br />
Preparing a novel board game `Canvas Time` to inform people from any distinct <br />
<br />
education level about features of our project, laboratory equipments and <br />
<br />
interesting Biobrick of iGEM competition.<br />
<br />
Meeting other iGEM teams from Turkey in SYNTHETIC BIOLOGY TURKEY MEETING.<br />
<br />
Stroving for the innovation and presentation of synthetic biology to people. As <br />
<br />
a medical school, our hospital has its own quarterly magazine, “Yasama Sanati” <br />
<br />
(in English: The Art of Living). <br />
<br />
Preparing a journal which is named “Canvas Times” and it gives you the actual <br />
<br />
news of some time to fun and talk with people about our project, synthetic <br />
<br />
biology and iGEM... Our all of activities and human practices are existed on our <br />
<br />
journal such as “iGEM Picnic” and “International Medical Students Conference. <br />
<br />
Performinf open-lab to public meetings such as gifted children studying in <br />
<br />
several schools of Ankara, undergraduate students from Hacettepe University <br />
<br />
Medical School and postgraduate students from Ankara University Medical School<br />
<br />
Kindergarten synthetic biology trip to teach synthetic biology to five-year-old <br />
<br />
children. <br />
<br />
COLLABORATION <br />
<br />
With friendly communications teams can take some useful advises and learn some <br />
<br />
important points. Also with helping to other groups friendly feelings increase <br />
<br />
and the team’s motivation increases. During our lab working we were always <br />
<br />
keeping touch with other teams. We were helping each other and making some <br />
<br />
activities together. <br />
<br />
We helped to Bilkent_UNAM_Turkey team when they needed our part 1001 and they <br />
<br />
helped to us with giving LB Agar. Also the Team Michigan asked helping to us <br />
<br />
about board game. We designed the Canvas Town Game and the Team Michigan wanted <br />
<br />
some points about preparing and designing. And we were being glad to help them.<br />
Association of Synthetic Biology – METU-Ankara Team invited our team to <br />
<br />
Synthetic Biology Turkey Meetings 1.0 of Igem Turkey Teams. This meeting was <br />
<br />
really funny and informative. <br />
<br />
We are planning a little tournament of Canvas Town Game in the Asia Religion <br />
<br />
Jamboree. For this organization we help from Religion Asia Organization <br />
<br />
Committee and they helped us very quickly and politely.<br />
<br />
For the tournament four teams will determine with drawing. 2 teams will play <br />
<br />
together and the other 2 teams will play together. Just 6 persons can play our <br />
<br />
game so every team should play with their 3 members. Then the winner teams will <br />
<br />
play together in the end of this game 3 finalist member of the last winner team <br />
<br />
will be presented.<br />
<br />
With these activities we were really enjoyed and informed.</p><br />
<br />
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Metugen
http://2011.igem.org/Team:Fatih_Turkey/Results
Team:Fatih Turkey/Results
2011-09-22T03:29:49Z
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<h2 style="font-family: Verdana, Arial, SunSans-Regular, sans-serif;">Project</h2><br />
<h5 style="font-family: Verdana, Arial, SunSans-Regular, sans-serif;">Results</h5> <br />
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<p><em>1. </em><em>technique;</em> in case of applying on surfaces, LALF protein can work like a new sterilization material like gluteraldehyte or alcohol that are commonly used for hospital and lab sterilization.<em> </em></p><br />
<p><em>2. </em><em>Alternative cure for septic shock;</em> after gram negative bacterial infections; LPS, which is the main component of cell wall of gram negative bacteria, can cause deathly conditions that is generally called “septic shock”. In our project, our LALF protein binds this material strictly, thus LALF can be used as an alternative cure method for such condition.<em> </em></p><br />
<p><em>3. </em><em>Possible new structure of antibiotic-like drug; </em>antibiotics commonly effect on production of cell wall of bacteria. This is done by inhibiting the source of production. On the other hand, our LALF protein directly binds the cell wall material and stops the bacteria growth. Thus, a new antibiotic like drug made of LALF may be produced and used in some infections.<em> </em></p><br />
<p><em>4. </em><em>Possibility of producing longtime sterile layers; </em>if any kind of object is produced by covering with LALF, that object can stay sterile for long time because of gram negative bacteria protection of LALF. In case of a possible infection on surface, LALF will stop bacteria growth.<em> </em></p><br />
<p><em>5. </em><em>Alternative cure method for bacterial diseases; </em>diseases like urinary system infection or gastritis are caused by gram negative bacteria.<em> </em>In the course of applying our bacteria that produce LALF in the infectious cite, infection of harmful bacteria can be prevented or stopped. This may suggest an alternative cure for such diseases.<em> </em></p><br />
<p>On the other hand, our secondary protein, reflectin, can be used in varying applications.</p><br />
<p><em>1. </em><em>Camouflage material; </em>this protein allows its host, cephalopod, to hide when cephalopod hunts or to defend itself from its hunters. In the course of development of reflectin protein, a new camouflage material may be produced for humans.<em> </em></p><br />
<p><em>2. </em><em>New material of dye for varying applications; </em>Dye that changes its color can be used almost every colorful application. From fireworks to wall paints, from windows of buildings to road signs, this ability can be very useful.<em></em></p><br />
<p><em>3. </em><em>Possibility of producing specialized light colors; </em>in some circumstances, a unique light color may be necessary in order to gain maximum output like in greenhouses or dark rooms in photography industry. Reflectin reflects the light by changing its wavelength; therefore the light that is reflected has only one color. This ability can be applied in such application that mentioned above.<em></em></p><br />
<p><strong><br /><br />
</strong></p><br />
<br />
<br />
<p><br />
For our project lab executions;<br />
<br />
LALF based anti-LPS factor system has been worked expectedly<br />
<br />
we have constructed and submitted 12 biobrick. Three devices working in two for <br />
<br />
B.subtilis one for E.coli work expectedly.<br />
<br />
Our B.subtilis specific promoter with SacB- extracellur signal- has been seen <br />
<br />
working well.<br />
<br />
We have synthesized Reflectin 1a protein in E.coli; however, because we did not <br />
<br />
have enough time to purify it to visualize via aid-eye, we could not use <br />
<br />
Reflectin 1a device as biosensor for our lalf-LPS interaction in B.subtilis-<br />
<br />
E.coli mixed culture. <br />
<br />
<br />
<br />
<br />
For Human Practice; We have performed the activities as follows;<br />
<br />
Providing Sporocide Method for prevention from spores of B.subtilis in further <br />
<br />
B.subtilis synthetic biology project.<br />
<br />
Preparing a novel board game `Canvas Time` to inform people from any distinct <br />
<br />
education level about features of our project, laboratory equipments and <br />
<br />
interesting Biobrick of iGEM competition.<br />
<br />
Meeting other iGEM teams from Turkey in SYNTHETIC BIOLOGY TURKEY MEETING.<br />
<br />
Stroving for the innovation and presentation of synthetic biology to people. As <br />
<br />
a medical school, our hospital has its own quarterly magazine, “Yasama Sanati” <br />
<br />
(in English: The Art of Living). <br />
<br />
Preparing a journal which is named “Canvas Times” and it gives you the actual <br />
<br />
news of some time to fun and talk with people about our project, synthetic <br />
<br />
biology and iGEM... Our all of activities and human practices are existed on our <br />
<br />
journal such as “iGEM Picnic” and “International Medical Students Conference. <br />
<br />
Performinf open-lab to public meetings such as gifted children studying in <br />
<br />
several schools of Ankara, undergraduate students from Hacettepe University <br />
<br />
Medical School and postgraduate students from Ankara University Medical School<br />
<br />
Kindergarten synthetic biology trip to teach synthetic biology to five-year-old <br />
<br />
children. <br />
<br />
COLLABORATION <br />
<br />
With friendly communications teams can take some useful advises and learn some <br />
<br />
important points. Also with helping to other groups friendly feelings increase <br />
<br />
and the team’s motivation increases. During our lab working we were always <br />
<br />
keeping touch with other teams. We were helping each other and making some <br />
<br />
activities together. <br />
<br />
We helped to Bilkent_UNAM_Turkey team when they needed our part 1001 and they <br />
<br />
helped to us with giving LB Agar. Also the Team Michigan asked helping to us <br />
<br />
about board game. We designed the Canvas Town Game and the Team Michigan wanted <br />
<br />
some points about preparing and designing. And we were being glad to help them.<br />
Association of Synthetic Biology – METU-Ankara Team invited our team to <br />
<br />
Synthetic Biology Turkey Meetings 1.0 of Igem Turkey Teams. This meeting was <br />
<br />
really funny and informative. <br />
<br />
We are planning a little tournament of Canvas Town Game in the Asia Religion <br />
<br />
Jamboree. For this organization we help from Religion Asia Organization <br />
<br />
Committee and they helped us very quickly and politely.<br />
<br />
For the tournament four teams will determine with drawing. 2 teams will play <br />
<br />
together and the other 2 teams will play together. Just 6 persons can play our <br />
<br />
game so every team should play with their 3 members. Then the winner teams will <br />
<br />
play together in the end of this game 3 finalist member of the last winner team <br />
<br />
will be presented.<br />
<br />
With these activities we were really enjoyed and informed.</p><br />
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Metugen
http://2011.igem.org/Team:Fatih_Turkey/Experiments
Team:Fatih Turkey/Experiments
2011-09-22T03:27:08Z
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<h2 style="font-family: Verdana, Arial, SunSans-Regular, sans-serif;">Project</h2><br />
<h5 style="font-family: Verdana, Arial, SunSans-Regular, sans-serif;">Experiments</h5> <br />
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<div style="font-size:15px; color: #333;"><br />
A)EXPERIMENTS<br />
<p style="padding-left: 30px;">1)<a href="#1">Disc experiments 1</a></p><br />
<p style="padding-left: 30px;">2)<a href="#2">Disc experiments 2</a></p><br />
<p style="padding-left: 30px;">3)<a href="#3">Sterilization period of Supernatant</a></p><br />
<p style="padding-left: 30px;">4)<a href="#4">The Suicide Experiment Of E. COLI</a></p><br />
<p style="padding-left: 30px;">5)<a href="#5">The Effect Of Feton Reant Solution On The Biofilm</a></p><br />
<p style="padding-left: 30px;">6)<a href="#6">The Difference between Colonies Containing and Not Containing Gene Parts of Reflectin Protein</a></p><br />
<p style="padding-left: 30px;">7)<a href="#7">Experiment of Supernatant</a></p><br />
<p style="padding-left: 30px;">8)<a href="#8">Experiment&nbsp;&nbsp;of liquid culture</a></p><br />
B)<a href="#B">CONFIRMATION</a><br />
<br><br />
C)<a href="#C">CHARACTERIZATION</a><br />
<br />
&nbsp;<br />
<br />
</div><br />
<br />
<br />
<span id="1"><strong><h1>Disc Experiment 1</h1></strong></span><br />
<p>We prepared an experiment like the disc experiments methods used to see antibiotic effectiveness, in order to see the effect of B. Subtillis with LALF on E. Coli.</p><br />
<p>By adding E. Coli on Bacillus Subtilis;</p><br />
<p><strong>Plate A1);</strong> We put E. Coli on the B. Subtilis with LALF (K541915) biofilm. For his purpose we first prepared acorn starch with liquid LB medium and added 100 ul B. Subtilis liquid medium as a point over it. For a qualified biofilm we incubated it for 24 hours at 37˚C.</p><br />
<p>Later we added 10 uL E. Coli with RFP as a single point and again incubated it.</p><br />
<p><strong>Plate A2);</strong> In order to understand whether LALF protein or another factor killed E. Coli on the Bacillus biofilm we prepared the same contrivance with a B. subtilis biofilm that doesn’t produce LALF protein or doesn’t have antibiotic resistance. So we could understand if the B. Subtilis biofilm itself not LALF, inhibited the growth of E. Coli.</p><br />
<p><strong>Plate A3);</strong>At this contrivancethe B. Subtilis we used for biofilm didn’t includeLALF protein but had antibiotic resistance. Why we didi this is we knew that there would be a color difference between Plate A1 and A2 but we woluld like to know if the antibiotic would prevent E. Coli with RFP from having color.</p><br />
<p><strong>Plate B1);</strong> We spreaded B. Subtilis with LALF over a normal lb medium. We put E. Coli as a single point while the Bacillus spread is fresh. We aimed to see if B. Subtilis could kill E. Coli without forming a biofilm.</p><br />
<p><strong>Plate B2);</strong> We prepared this plate as a control plate of Plate C. For thşs we prepared the same contrivance with only B Subtilis that doesn’t produce LALF protein or doesn’t have antibiotic resistance in order to be sure that if the EColi of the plate B1 does not grow the reason for this is LALF protein.</p><br />
<p><strong>Plate B3);</strong> In this contrivance we spread B. Subtilis that doesn’t produce LALAF protein but has antibiotic resistance and added E.Coli with RFP as a single drop over it.</p><br />
<p>By adding E. Coli over B. Subtilis ;</p><br />
<p><strong>Plate C1);</strong> We added 100 ul liquid culture of B. Subtilis with LAFL (k541915), over the normal lg medium applied with E. Coli with RFP,as a single drop.</p><br />
<p><strong>Plate C2);</strong> Over the fresh spread of E. Coli, 100 ul of liquid culture of B. Subtiis not producing LALF or doesn’t have antibiotic resistance as a single drop.</p><br />
<p><strong>Plate C3);</strong> At this contrivance we added B. Subtilis that doesn’t produce LALF but has antibiotic resistance over the fresh spread of E. Coli with RFP likewise. We aimed to see the affect of antibiotic resistance over E. Coli with RFP ,by using B. subtilis with and without antibiotic resistance.</p><br />
<p><strong>Plate D1);</strong> We added supernatant of B. Subtilis with LALF as a single drop over fresh spread of E. Coli with RFP. Because at the beginning of the LALF part of B. Subtilis there was a throwing out signal and we expected death mostly here.</p><br />
<p><strong>Plate D2);</strong> At this plate we applied 100 ul of supernatant of B: subtilis not producing LALF protein and not having antibiotic resistance over the fresh spread of E. Coli as a single drop.</p><br />
<p><strong>Plate D3);</strong> At this plate we applied 100 ul of supernatant of B: subtilis not producing LALF protein and but that has antibiotic resistance over the fresh spread of E. Coli as a single drop.</p><br />
<p><img src="https://static.igem.org/mediawiki/2011/d/d1/Sss1.png"></p><br />
<p>Plates B2 and B1 (Respectively)</p><br />
<p><img src="https://static.igem.org/mediawiki/igem.org/f/fd/Sss2.png"></p><br />
<p>Plates C1 and C2 (Respectively)</p><br />
<p><img src="https://static.igem.org/mediawiki/2011/8/85/Sss3.png" ></p><br />
<p>Plates D2 and D1</p><br />
<p><strong><span style="text-decoration: underline;"><br /><br />
</span></strong></p><br />
<br />
<br />
<br />
<p><strong><span id="2"><h1>DISC EXPERIMENT 2</h1></span></strong></p><br />
<p>We have prevented E.coli growth in our experiment. We took antibiotic disks which are used in hospitals.</p><br />
<p>We put antibiotic disks onto spreads of E.coli which produces RFP protein and has the chloramphenicol resistance. Then, we added different amounts of supernatant.</p><br />
<p>We used supernatant with LALF and without LALF of B.subtilis. We centrifuged LB broth culture which was incubated for 12 hours at 37°C at 7000 rpm for 5 minutes. It is filtered with 0,2 filter, afterwards.</p><br />
<p><img src="https://static.igem.org/mediawiki/2011/f/f9/IMG_0331.JPG"></p><br />
<p>E.coli with RFP was spread on both of two plates. 40ul, 80ul, 120ul and 160ul supernatant of B.subtilis which do not include LALF protein was added into left plate. Then 40ul, 80ul, 120ul and 160 supernatant of B.subtilis which include LALF protein was added into right plate .We got results which support other experiments.</p><br />
<p><img src="https://static.igem.org/mediawiki/2011/1/1f/IMG_0335.JPG"></p><br />
<p>&nbsp;</p><br />
<p><img src="https://static.igem.org/mediawiki/2011/a/a6/IMG_0339.JPG"></p><br />
<p>Supernatants with and without LALF protein was put onto 50 ul spread of E.coli with RFP in declared amount at the picture .Again we got results which is overlapped other our experiments.</p><br />
<p>The reason of making this experiment with antibiotic and without antibiotic plate was if antibiotic effects LALF or not?</p><br />
<p><strong><br /><br />
</strong></p><br />
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<p><strong><h1><span id"3">Sterilization period of Supernatant</span></h1></strong></p><br />
<p>We have deduced that supernatant of B.subtilis that produces LALF sterilize the surfaces that LALF had applied on. Thus, we wanted to test how long this sterilization exists. To do this, supernatants with and without LALF waited in +4 ⁰C for 24 and 36 hours. Then, we tested their ability to sterilize.</p><br />
<p><img src="https://2011.igem.org/File:Mustafa1.JPG"></p><br />
<p>Equal amount of E.coli with RFP sequence were spread into the fresh plates Then, 100 ul supernatant with and without was added on plates. Note that no colony grew on where supernatant that contents LALF was added after both 24 and 36 hours. (the plates on the right)</p><br />
<p><img src="https://static.igem.org/mediawiki/2011/1/10/Mustafa2.JPG"></p><br />
<p>To understand better how much effective our protein is, we measured the diameter of the no growth zone. In 24 hour waited plate, diameter was 2,5 cm; on the other hand, it was measured 1,7 cm in 36 hour waited plate.</p><br />
<p>&nbsp;</p><br />
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<h1><strong><span id="4">THE SUICIDE EXPERIMENT OF E. COLI</span></strong></h1><br />
<p><em>Our K541545 part contains IPTG promoter and the gene part of LALF protein on a backbone which has resistance to chloramphenicol. We transported this part to E. coli for testing whether E. coli would kill itself or not.</em></p><br />
<p><strong>Experiments of plates with and without IPTG<em> </em></strong></p><br />
<p>Plate A1: We streaked E coli culture which has K541545 on this plate and the plate includes IPTG and Chloramphenicol.</p><br />
<p>Plate A2: We streaked E coli culture which has K541545 on this plate and the plate includes just Chloramphenicol.</p><br />
<p>Plate B1: This plate does not have IPTG and we streaked E.coli culture which does not have IPTG promoter.</p><br />
<p>PlateB2: This plate has IPTG and we streaked E coli culture which has IPTG promoter.</p><br />
<p>We prepared the B1 and B2 plates for testing toxic effect of IPTG. Because we wanted to be sure that the only factor affecting growth of E coli is LALF protein.</p><br />
<p>&nbsp;</p><br />
<p><strong> </strong></p><br />
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<p><strong><span id="5"><h1>THE EFFECT OF FENTON REAGENT SOLUTION ON THE BIOFILM</h1></span></strong></p><br />
<p>Fenton Reagent solution is a solution that can kill the B. Subtilis spors and bacteria. It kills the spors by oxidizing with its Cu ions.</p><br />
<p>We prepared this solution in two ways one with the hydrogen peroxide (OFR) according to the literature and the other with distilized water (DFR). In our experiments we learned which of these two solutions is more effective on E. Coli, B. Subtilis or the the spores of B. Subtilis and degree of its effectiveness.</p><br />
<p>The aim of this experiment is seeing the effects of the fenton reagen solution on the B. Subtilis and E. Coli on the biofilms.</p><br />
<p>We prepared Corn Strach Lb Agar for a more qualified biofilm. In order to understand the death in the plates we added a small amount to the liquid culture.</p><br />
<p>Plate 1: On this plate OFR is added on the B. Subtilis biofilm</p><br />
<p>Plate 2: We formed another B. Subtilis biofilm and added same amount of DFR on it.</p><br />
<p>Plate 3: On this contrivance one drop of E. Coli with RFP on the B. Subtilis biofilm and added same amount of DFR on it</p><br />
<p>Plate 4: On this contrivance one drop of E. Coli with RFP on the B. Subtilis biofilm and added same amount of OFR on it</p><br />
<p>Plate 5: this plate prepared as a control group of 3<sup>rd</sup> and 4<sup>th</sup> plates. On this contrivance one drop of E. Coli with RFP on the B. Subtilis biofilm but any kind of fenton reagent solutions didn’t add on it.</p><br />
<p>RESULT: After adding the FR solutions on all of plates, we measured their OD values in the end of this experiment we measured again and saw that the 1<sup>st</sup>, 2<sup>nd</sup>, 3<sup>rd</sup>, 4<sup>th</sup> plates OD values didn’t change. But the the OD value of 5<sup>th</sup> plate increased. 5<sup>th</sup> plate is the control group.</p><br />
<p>&nbsp;</p><br />
<p>&nbsp;</p><br />
<p><img src="https://static.igem.org/mediawiki/igem.org/6/6e/S1.png"></p><br />
<p>Figur2: plate 3 and plate 4</p><br />
<p><img src="https://static.igem.org/mediawiki/2011/4/40/S2.png"></p><br />
<p><img src="https://static.igem.org/mediawiki/2011/a/af/S3.png"></p><br />
<br />
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<p><strong><span id="6"></span></strong></p><h1><strong>The Difference between Colonies Containing and Not Containing Gene Parts of Reflectin Protein</strong></h1><p></p><br />
<p>We transfered the plasmid which includes the gene part of reflectin protein (j04450 shuttle, j04500 backbone, and 1006) to E. Coli and we saw that there is a difference between reflectin including colonies and the colonies which does not include reflectin protein.</p><br />
<p>We saw that the plate which does not include reflectin protein is normally transparent. But the other plate which includes reflectin protein seemed white. And this is showing us that our plasmid is working and the protein is produced by E. coli.<br><br />
Figure-1: the difference of colonies is showing clearly, the colonies at the right side are seemed white and the colonies at the left side are seemed transparent.</p><br />
<p><img src="https://static.igem.org/mediawiki/igem.org/3/34/Aaa1.png"></p><br />
<p><img src="https://static.igem.org/mediawiki/igem.org/1/14/Aaa2.png"></p><br />
<p>Additionally, the colonies are looked into by microscope. The colony which is including reflectin has some transparent points. But the control group hasn’t these points. We are thinking that because of reflectin protein there are the points in these colonies.</p><br />
<p>&nbsp;</p><br />
<p><img src="https://static.igem.org/mediawiki/igem.org/0/09/Aaa3.png"><br><img src="https://static.igem.org/mediawiki/igem.org/9/9a/Aaa4.png"></p><br />
<p>Figure-4: Image of the colonies which are include reflectin protein by microscope it’s clearly showing that the transparent points in the colonies</p><br />
<p>&nbsp;</p><br />
<p>Figure-6: Image of the control group which is not including the 1006 gene part and reflectin protein and it can clearly showing the difference from the colonies which include reflectin protein. There aren’t the transparent points here.</p><br />
<p><img src="https://static.igem.org/mediawiki/igem.org/0/0f/Aaa5.png"></p><br />
<p>Consequently, it’s confirmed that the 1006 gene part is producing the reflectin protein</p><br />
<p>&nbsp;</p><br />
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<div><br />
<p></p><h1><strong><span id="7">Experiment of Supernatant</span></strong></h1><p></p><br />
</div><br />
<p>We wanted to investigate to effects of supernatant includes LALF protein to E.coli in this experiment.</p><br />
<p>Therefore we put 8 ul LB broth into falcons.then we added them E.coli which has rfp and incubated for 12 hours in 37⁰C .We put pure E.coli into only one falcon for control.We added 1ul,10ul and 100ul supernatant with LALF and without LALF.</p><br />
<p>We diluated them 7 times 2-4-8-12hours later then we spreaded 10ul to plates which has 1/0,5 l Cloramphenicol.We did santrifuge liquid culture which incubated for 12 hours at 37⁰C at 7000 rpm for 5 minutes because of preparing supernant with LALF and without LALF.Then we passed them from the 0,2 filter.</p><br />
<p>Plates were spreaded after 2 hours later:</p><br />
<p><img src="https://static.igem.org/mediawiki/igem.org/5/5f/2saat.jpg"></p><br />
<p>1a)We added 1ul supernatant includes LALF into left plate and 1ul supernatant without LALF into right plate</p><br />
<p><img src="https://static.igem.org/mediawiki/igem.org/1/12/2saat_b.jpg"></p><br />
<p>1b)We added 10 ul supernatant includes LALF into left plate and 10 ul supernatant without LALF into right plate</p><br />
<p><img src="https://static.igem.org/mediawiki/igem.org/d/db/2_saat_c.jpg"></p><br />
<p>1c)We added 100ul supernatant includes LALF into left plate and 100ul supernatant without LALF into right plate</p><br />
<p>Plates were spreaded after 4 hours later;</p><br />
<p><img src="https://static.igem.org/mediawiki/igem.org/9/9d/4saat_a.jpg"></p><br />
<p>2a)We added 1ul supernatant includes LALF into left plate and 1ul supernatant without LALF into right plate.</p><br />
<p><img src="https://static.igem.org/mediawiki/igem.org/9/9a/4saat_b.jpg"></p><br />
<p>2b)We added 100ul supernatant includes LALF into left plate and 100 ul supernatant without LALF into right plate.</p><br />
<p>Plates were spreaded after 8 hours later;</p><br />
<p><img src="https://static.igem.org/mediawiki/igem.org/e/e7/8_saat_a.jpg"></p><br />
<p>3a)We added 1ul supernatant includes LALF into left plate and 1ul suoernatant without LALF into right plate.</p><br />
<p><img src="https://static.igem.org/mediawiki/igem.org/c/ca/8_saat_b.jpg"></p><br />
<p>3b)We added 10 ul supernatant includes LALF into left plate and 10 ul supernatant without LALF into right plate.</p><br />
<p><img src="https://static.igem.org/mediawiki/igem.org/d/de/8_saat_c.jpg"></p><br />
<p>3c) We added 100ul supernatant includes LALF into left plate and 100 ul supernatant without LALF into right plate.</p><br />
<p>Plates were spreaded after 12 hours later;</p><br />
<p><img src="https://static.igem.org/mediawiki/igem.org/1/19/12_saat_a.jpg"></p><br />
<p>4a) We added 1ul supernatant includes LALF into left plate and 1ul suoernatant without LALF into right plate.</p><br />
<p><img src="https://static.igem.org/mediawiki/igem.org/2/2b/12_saat_b.jpg"></p><br />
<p>4b)We added 10 ul supernatant includes LALF into left plate and 10 ul supernatant without LALF into right plate.</p><br />
<p><img src="https://static.igem.org/mediawiki/igem.org/f/fc/12_saat_c.jpg"></p><br />
<p>4c)We added 100ul supernatant includes LALF into left plate and 100 ul supernatant without LALF into right plate.</p><br />
<p><em><br><br />
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<h3><strong><span id="8"><h1>Experiment of liquid culture</h1></span></strong></h3><br><br />
&nbsp;&nbsp;&nbsp;We wanted to see the effects of supernatant of B.subtilis which produces LALF in order to stop e.coli with RFP growth in liquid culture.<br><br />
&nbsp;&nbsp;&nbsp;We put 8 ml LB broth in falcons.<br><br />
&nbsp;&nbsp;&nbsp;We planned 3 control groups.These are:<br />
<ul><br />
<li>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Control group 1:Includes only 5ul e.coli which waited for 12 hours in 37⁰C. These can synthesize RFP.</li><br />
<li>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Control group 2:Includes only 5 ul b.subtilis which produces LALF. They waited for 12 hours in 37⁰C.</li><br />
<li>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Control group 3:Includes only 5ul b.subtilis which do not produce LALF. They waited for 12 hours in 37⁰C.</li><br />
</ul><br />
&nbsp;&nbsp;&nbsp;First of all, we put 5ul e.coli which has RFP into 8 falcons as experimental groups. We waited experimental falcons for 12 hours in 37⁰C . Then, we added 1ul,10ul,100ul and 1000ul liquid culture with LALF and liquid culture without LALF. After this, we incubated them in 37⁰C and measured their OD value 2-4-8-12 hours later in 405 nm. Their results are as below:<br><br />
<img src="https://static.igem.org/mediawiki/2011/e/ee/Graphicexp1.jpg"><br><br />
Graph1 : Absorbance(OD405) value difference through time of AntiLPS-LPS binding with 1 ul LALF<br><br />
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<img src="https://static.igem.org/mediawiki/2011/f/fb/Graphicexp2.jpg"><br><br />
Graph2 : Absorbance(OD405) value difference through time of AntiLPS-LPS binding with 10 ul LALF<br><br />
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<img src="https://static.igem.org/mediawiki/2011/f/f1/Graphicexp3.jpg"><br><br />
Graph3 : Absorbance(OD405) value difference through time of AntiLPS-LPS binding with 100 ul LALF<br><br />
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<img src="https://static.igem.org/mediawiki/2011/b/b4/Graphicexp4.jpg"><br><br />
Graph4 : Absorbance(OD405) value difference through time of AntiLPS-LPS binding with 1000 ul LALF<br><br />
<hr><br><br />
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<br><hr><span id="B"><br />
<h1><strong>CONFIRMATION</strong></h1><br />
<img src="https://static.igem.org/mediawiki/igem.org/d/db/M1.jpg"></span><br><br />
1: This part includes a gram positive promoter, an RBS sequence and SacB signal peptide sequence to synthesize the protein outside of the cell. This image shows that indicated part is considered as confirmed.<br />
<hr><br />
<img src="https://static.igem.org/mediawiki/2011/c/cd/M2.jpg"><br><br />
2:This part includes a gram positive promoter, an RBS sequence and LipA signal peptide sequence to synthesize the protein outside of the cell. This image shows that indicated part is considered as confirmed.<br />
<hr><br />
<img src="https://static.igem.org/mediawiki/2011/7/77/M3.jpg"><br><br />
3: This part includes a constitutive promoter, an RBS sequence and Tat signal peptide sequence to synthesize the protein outside of the cell. This image shows that indicated part is considered as confirmed.<br />
<hr><br />
<img src="https://static.igem.org/mediawiki/2011/5/5d/M4.jpg"><br><br />
4:This part includes a IPTG inducible promoter, an RBS sequence and Tat signal peptide sequence to synthesize the protein outside of the cell. This image shows that indicated part is considered as confirmed.<br />
<hr><br />
<img src="https://static.igem.org/mediawiki/2011/4/49/M5.jpg"><br><br />
5:Our “LALF (limulus anti-lipopolysaccharide factor)” protein allows stopping any kind of gram negative bacteria growth by binding their cell wall material, LPS. This image shows that indicated part is considered as confirmed.<br />
<hr><br />
<img src="https://static.igem.org/mediawiki/2011/6/6c/M6.jpg"><br><br />
6:Our “reflectin” protein has the ability to reflect the light by changing its wavelength, thus its color. In our project, we planned to use this protein as an indicator on E.coli whether our LALF protein works properly or not. This image shows that indicated part is considered as confirmed.<br />
<hr><br />
<img src="https://static.igem.org/mediawiki/2011/6/6c/M7.jpg"><br><br />
8:This part is a well-studied gram positive promoter. We planned to use this gene in the case of our signal peptide sequences do not work. When the protein, which is attached to this gene, is produced, we planned to blow up the bacteria; therefore synthesized protein would be in supernatant. This image shows that indicated part is considered as confirmed.<br />
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<hr><br />
<img src="https://static.igem.org/mediawiki/igem.org/0/07/M8.jpg"><br><br />
500: To perform cloning perfectly, we used pSB1C3 in E.coli. This vector is also the main vector for our parts that works only in E.coli. All of our parts are also inserted into this vector; because it is declared that all parts must be sent to Registry in pSB1C3. This image shows that this part is confirmed.<br />
<br />
<hr><br />
<img src="https://static.igem.org/mediawiki/2011/c/c0/Ms4.png"><br><br />
501: To perform ligation procedure appropriately, we designed an alternative part for all of our material parts. We inserted our promoters and proteins into pSB1C3 in order to have the alternative part that possesses different antibiotic resistance, comparing to original vectors of the parts. Moreover, it is wanted that all parts must be sent to Registry in pSB1C3. This part includes a gram positive promoter, RBS sequence and SacB signal peptide sequence. In the image, it can be seen that this part is confirmed.<br />
<br />
<hr><br />
<img src="https://static.igem.org/mediawiki/2011/6/61/M10.jpg"><br><br />
502: To perform ligation procedure appropriately, we designed an alternative part for all of our material parts. We inserted our promoters and proteins into pSB1C3 in order to have the alternative part that possesses different antibiotic resistance, comparing to original vectors of the parts. Moreover, it is wanted that all parts must be sent to Registry in pSB1C3. This part includes a gram positive promoter, RBS sequence and LipA signal peptide sequence. In the image, it can be seen that this part is confirmed.<br />
<br />
<hr><br />
<img src="https://static.igem.org/mediawiki/2011/4/48/M11.jpg"><br><br />
505:To perform ligation procedure appropriately, we designed an alternative part for all of our material parts. We inserted our promoters and proteins into pSB1C3 in order to have the alternative part that possesses different antibiotic resistance, comparing to original vectors of the parts. Moreover, it is wanted that all parts must be sent to Registry in pSB1C3. This part includes the sequence of our LALF protein. In the image, it can be seen that this part is confirmed.<br />
<br />
<hr><br />
<img src="https://static.igem.org/mediawiki/2011/a/aa/M12.jpg"><br><br />
506:To perform ligation procedure appropriately, we designed an alternative part for all of our material parts. We inserted our promoters and proteins into pSB1C3 in order to have the alternative part that possesses different antibiotic resistance, comparing to original vectors of the parts. Moreover, it is wanted that all parts must be sent to Registry in pSB1C3. This part includes the sequence of our reflectin protein. In the image, it can be seen that this part is confirmed.<br />
<br />
<hr><br />
<img src="https://static.igem.org/mediawiki/2011/2/25/M13.jpg"><br><br />
800: In our project, we planned to use gram positive bacteria to synthesize our LALF protein in order to stop gram negative growth. On the other hand, to identify effectively whether our protein works or not, we decided to use reflectinprotein and to produce it from both of our bacteria; B.subtilis and E.coli. Thus, we determined to use a shuttle vector that works both bacteria kinds. This vector has ampicillin resistance for E.coli and chloramphenicol resistance for B.subtilis. In the image, it can be seen that this part is confirmed.<br />
<br />
<hr><br />
<img src="https://static.igem.org/mediawiki/2011/a/a0/Ms3.png"><br><br />
900:In our project, we planned to use gram positive bacteria to synthesize our LALF protein in order to stop gram negative growth. On the other hand, to identify effectively whether our protein works or not, we decided to use reflectin protein and to produce it from both of our bacteria; B.subtilis and E.coli. Thus, we determined to use a shuttle vector that works both bacteria kinds. This vector has chloramphenicol resistance for both of bacteria kinds. This part also includes an RFP sequence. In the image, it can be seen that this part is confirmed.<br />
<hr><br />
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<img src="https://static.igem.org/mediawiki/igem.org/a/a5/M16.jpg"><br><br />
545: This part is designed; because it is wanted that all parts must be sent to headquarters in pSB1C3 vector. It possesses a promoter that works in gram negative bacteria, Tat signal sequence in order to synthesize the protein outside and LALF protein gene. In this image, indicated part is considered as confirmed.<br />
<hr><br />
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<img src="https://static.igem.org/mediawiki/2011/6/64/Ms2.png"><br><br />
815: In order to stop gram negative bacteria growth, our LALF protein must be synthesized by another type of bacteria, gram positive. Thus, we designed a special gene that works in only gram positive bacteria by inserting a promoter specialized for gram positive. Also, SacB signal sequence that allows us synthesizing the protein outside and LALF protein gene is included.All components are inserted into 800 that isdesigned for gram positive. In this image, indicated part is considered as confirmed<br />
<hr><br />
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<img src="https://static.igem.org/mediawiki/2011/6/6c/Ms1.png"><br><br />
915: In order to stop gram negative bacteria growth, our LALF protein must be synthesized by another type of bacteria, gram positive. Thus, we designed a special gene that works in only gram positive bacteria by inserting a promoter specialized for gram positive. Also, SacB signal sequence that allows us synthesizing the protein outside and LALF protein gene is included.All components are inserted into 900 that is designed for gram positive. In this image, indicated part is considered as confirmed.<br />
<hr><br />
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<img src="https://static.igem.org/mediawiki/2011/5/54/M18.jpg"><br><br />
925:In order to stop gram negative bacteria growth, our LALF protein must be synthesized by another type of bacteria, gram positive. Thus, we designed a special gene that works in only gram positive bacteria by inserting a promoter specialized for gram positive. Also, LipA signal sequence that allows us synthesizing the protein outside and LALF protein gene is included.All components are inserted into 900 that is designed for gram positive. In this image, indicated part is considered as confirmed.<br />
<br />
<hr><br />
<br />
<img src="https://static.igem.org/mediawiki/2011/3/30/M19.jpg"><br><br />
516: This part is designed; because all parts must be sent to headquarters in pSB1C3 vector. In this image, indicated part is considered as confirmed.<br />
<br />
<hr><br />
<br />
<img src="https://static.igem.org/mediawiki/2011/5/5c/M20.jpg"><br><br />
526: This part is designed; because all parts must be sent to headquarters in pSB1C3 vector.In this image, indicated part is considered as confirmed.<br />
<br />
<hr><br />
<br />
<img src="https://static.igem.org/mediawiki/2011/d/d9/M21.jpg"><br><br />
536:In our experiments, we aimed to indicate whether our LALF protein works or not, we used an alternative indicator protein, reflectin. This part is designed to work in gram negative E.coli. A constitutive promoter, a Tat signal sequence that allows synthesizing the protein outside and reflectin protein gene is included in pSB1C3. In this image, indicated part is considered as confirmed.<br />
<br />
<hr><br />
<br />
<img src="https://static.igem.org/mediawiki/2011/3/3b/M22.jpg"><br><br />
546:In our experiments, we aimed to indicate whether our LALF protein works or not, we used an alternative indicator protein, reflectin. This part is designed to work in gram negative E.coli. An IPTG inducible promoter, a Tat signal sequence that allows synthesizing the protein outside and reflectin protein gene is included in pSB1C3. In this image, indicated part is considered as confirmed.<br />
<br />
<hr><br />
<br />
<img src="https://static.igem.org/mediawiki/igem.org/8/85/M23.jpg"><br><br />
596:In our experiments, we aimed to indicate whether our LALF protein works or not, we used an alternative indicator protein, reflectin. We designed an alternative part in the case that our signal sequences do not work. This part includes a promoter that works in only gram negative and reflectin protein gene. All components are inserted into pSB1C3. In this image, indicated part is considered as confirmed.<br />
<br />
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<p><strong><span id="C"><h1>CHARACTERIZATION OF LALF PROTEIN</h1></span></strong></p><br />
<p>&nbsp;</p><br />
<p>TARGET: Showing that the LALF protein is produced.</p><br />
<p>&nbsp;</p><br />
<p>1)</p><br />
<p>&nbsp;</p><br />
<p><img src="https://static.igem.org/mediawiki/igem.org/1/17/F_1.png"></p><br />
<p>&nbsp;</p><br />
<p><img src="https://static.igem.org/mediawiki/2011/e/ee/Karakterizasyon2.jpg"></p><br />
<p>&nbsp;</p><br />
<p>Transformation is done and green colonies are selected then we started to do our second procedure.</p><br />
<p>&nbsp;</p><br />
<p><img src="https://static.igem.org/mediawiki/2011/0/04/Karakterizasyon3.jpg"></p><br />
<p>&nbsp;</p><br />
<p>2)</p><br />
<p>&nbsp;</p><br />
<p><img src="https://static.igem.org/mediawiki/2011/4/42/F4.png"></p><br />
<p>&nbsp;</p><br />
<p><img src="https://static.igem.org/mediawiki/2011/e/eb/Karakterizasyon4.jpg"></p><br />
<p>&nbsp;</p><br />
<p><img src="https://static.igem.org/mediawiki/2011/b/bd/Karakterizasyon5.jpg"></p><br />
<p><strong><span style="text-decoration: underline;"><br /><br />
</span></strong></p><br />
<br />
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<p><strong><span style="text-decoration: underline;">TARGET</span></strong><strong>: The characterization of part 545 which is done suicide experiment of E.coli</strong></p><br />
<p><strong>1) </strong><strong>The sequence of part 545</strong></p><br />
<p>Procedures of liquid culture which is planted by cloning team:</p><br />
<p>-PCR</p><br />
<p><em> -Existence of expected plasmid is confirmed by electrophoresis.</em></p><br />
<p><em>-Sequence</em></p><br />
<p><strong>2) </strong><strong>Part 545 c DNA sequence</strong></p><br />
<p>Procedures of liquid culture which is planted by cloning team:</p><br />
<p>Plasmid isolation</p><br />
<p>BL21 transformation</p><br />
<p>Liquid culture</p><br />
<p>RNA isolation</p><br />
<p>C DNA</p><br />
<p>PCR</p><br />
<p><em>Producing of expected part’s mRNA is confirmed by electrophoresis.</em></p><br />
<p>Sequence</p><br />
<p>&nbsp;</p><br />
<p><img src="https://static.igem.org/mediawiki/igem.org/a/af/S3.png"></p><br />
<p><strong><span style="text-decoration: underline;">RESULT</span></strong><strong>: LALF find in the DNA sequence and mRNA sequence. We produced LALF protein by E. coli</strong>.<strong> </strong></p><br />
<p>&nbsp;</p><br />
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Team:Fatih Turkey/Team
2011-09-22T02:50:05Z
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<big>ESRA GÜNDÜZ</big><br/>(Associate Professor of Medical Genetics)<br />
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<p>Hello everyone! My name is Esra. I’m an Associate Professor of Medical Genetics. This is my third year back in Turkey after staying twelve years in Japan. I mainly work on Cancer Genetics and more specifically on Tumor Suppressor Genes. I do enjoy being with my students and working with them together. iGEM competition has provided us to become closer. I think we share a lot scientifically, socially and friendly together with my students. I also like speaking Japanese and cooking.</p><br />
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<big>KADİR DEMİRCAN</big><br/>(Assistant Professor of Medical Genetics)<br />
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<td>&nbsp;<br />
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<big>HÜSAMETTİN ERDAMAR</big><br/>(Assistant Professor of Medical Biochemistry)<br />
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<td>&nbsp;</td><br />
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</tr><br />
<br />
<tr style="height: 70px;vertical-align: top;"><th colspan="2" style="text-align: center; font-size: 20px; font-weight: bold;">ADVISORS</th></tr><br />
<br />
<tr style="height: 200px;" id="13"><br />
<td><br />
<img src="https://static.igem.org/mediawiki/2011/c/c1/Cihantastan.jpg"/><br />
</td><br />
<td width="600" style="padding-left: 20px; padding-bottom: 20px;"><br />
<table style="margin-top:0px;"><br />
<tr><td><br />
<big>CİHAN TAŞTAN</big><br/>(Ph.D Student on Tumor Immunology at Sackler Institue of New York University)<br />
</td></tr><br />
<tr><br />
<td><br />
<p> I am graduate student for Ph.D. on Tumor Immunology at Sackler Institue of New York University. I have participated iGEM competition several times. I have taken parts in the competition as a student member of METU-Gene 2009 (Silver), METU-Turkey Software 2010 (Silver) and METU-Turkey 2010 (Gold). Now, I have been advisor of Fatih University for couple of months in order to start integration of other universities of Turkey into iGEM and to prepare an environment for establisihing and spreading of new synthetic biology labs through Turkey.</p><br />
</td><br />
</tr><br />
</table><br />
</td><br />
</tr><br />
<br />
<tr style="height: 200px;" id="14"><br />
<td><br />
<img src="https://static.igem.org/mediawiki/2011/e/e3/Muradiye.jpg"/><br />
</td><br />
<td width="600" style="padding-left: 20px; padding-bottom: 20px;"><br />
<table style="margin-top:0px;"><br />
<tr><td><br />
<big>MURADİYE ACAR</big><br/>(Ph.D Student on Mecdical Genetics)<br />
</td></tr><br />
<tr><br />
<td><br />
&nbsp; </td><br />
</tr><br />
</table><br />
</td><br />
</tr><br />
<br />
<tr style="height: 20px;vertical-align: top;"><th colspan="2" style="text-align: center; font-size: 20px; font-weight: bold;">ATTRIBUTION</th></tr><br />
<br />
<div><tr style="height:100px;"><td colspan="2"> Canvas Town is a proud product of Fatih Turkey group where all work presented on this wiki is the sole work of the iGEM team members listed below.<br />
All project executions have been performed by medical school team members through the summer. They have also performed all activities of Human practice.<br />
Additionally all the logo, illustrations, cartoons, other visual media and web design has been created by the design team in the group.We are also extremely thankful for the help of advisors and instructors just above.<br />
</td></tr></div><br />
<br />
<br />
<tr style="height: 70px;vertical-align: top;"><th colspan="2" style="text-align: center; font-size: 20px; font-weight: bold;">STUDENTS</th></tr><br />
<br />
<tr style="height: 200px;" id="1"><br />
<td><br />
<img src="https://static.igem.org/mediawiki/2011/0/0a/Alperen.jpg"/><br />
</td><br />
<td width="600" style="padding-left: 20px; padding-bottom: 20px;"><br />
<table style="margin-top:0px;"><br />
<tr><td><br />
<big>ALPEREN GÜVER</big><br/>Medical Student (2nd Year)<br />
</td></tr><br />
<tr><br />
<td><br />
<p>I am a first term student of Fatih University Medical Faculty and I will finish first year on July. When I finish my faculty, I want to be a clinician. I like playing tennis and taking some photos.</p><br />
</td><br />
</tr><br />
</table><br />
</td><br />
</tr><br />
<br />
<tr style="height: 200px;" id="2"><br />
<td><br />
<img src="https://static.igem.org/mediawiki/2011/f/f7/Betul.jpg"/><br />
</td><br />
<td width="600" style="padding-left: 20px; padding-bottom: 20px;"><br />
<table style="margin-top:0px;"><br />
<tr><td><br />
<big>BETÜL GÜNBEY</big><br/>Medical Student (2nd Year)<br />
</td></tr><br />
<tr><br />
<td><br />
<p>I’m a first year student in medical faculty. Also this is my first year in iGEM, I’m interested in clinical medicine; but also, I always like genetics and working in the lab. I think iGEM is a perfect way to learn synthetic biology and teamwork for a student. I like playing guitar. I’m sure our team will be very successful.</p><br />
</td><br />
</tr><br />
</table><br />
</td><br />
</tr><br />
<br />
<tr style="height: 200px;" id="3"><br />
<td><br />
<img src="https://static.igem.org/mediawiki/2011/0/0f/Hasanyasin.jpg"/><br />
</td><br />
<td width="600" style="padding-left: 20px; padding-bottom: 20px;"><br />
<table style="margin-top:0px;"><br />
<tr><td><br />
<big>HASAN YASİN SOYLU</big><br/>Medical Student (2nd Year)<br />
</td></tr><br />
<tr><br />
<td><br />
<p>I'm happy to be a part of the team. There is nothing to say about the past time; but I'm here for the future.<br />
"Bismillah is the beginning of every good thing. So, let's say Bismillah!.."</p><br />
</td><br />
</tr><br />
</table><br />
</td><br />
</tr><br />
<br />
<tr style="height: 200px;" id="4"><br />
<td><br />
<img src="https://static.igem.org/mediawiki/2011/7/7a/Team-fazilet.jpg"/><br />
</td><br />
<td width="600" style="padding-left: 20px; padding-bottom: 20px;"><br />
<table style="margin-top:0px;"><br />
<tr><td><br />
<big>FAZİLET GÜLER</big><br/>Medical Student (3rd Year)<br />
</td></tr><br />
<tr><br />
<td><br />
<p>I finished my second year in medical school on June. I'll be a doctor but I don't want to be a clinician. Thus, genetics and synthetic biology are treasure for me:) This is my first year in iGEM. I'm interested in physics and computer science. I like traveling and hiking. Also, I enjoy riding a roller coaster!</p><br />
</td><br />
</tr><br />
</table><br />
</td><br />
</tr><br />
<br />
<tr style="height: 200px;" id="5"><br />
<td><br />
<img src="https://static.igem.org/mediawiki/2011/9/95/Kenangzl.jpg"/><br />
</td><br />
<td width="600" style="padding-left: 20px; padding-bottom: 20px;"><br />
<table style="margin-top:0px;"><br />
<tr><td><br />
<big>KENAN OĞUZ GÜZEL</big><br/>Cartoonist<br />
</td></tr><br />
<tr><br />
<td><br />
&nbsp;<br />
</td><br />
</tr><br />
</table><br />
</td><br />
</tr><br />
<br />
<tr style="height: 200px;" id="6"><br />
<td><br />
<img src="https://static.igem.org/mediawiki/2011/d/d0/Hale.jpg"/><br />
</td><br />
<td width="600" style="padding-left: 20px; padding-bottom: 20px;"><br />
<table style="margin-top:0px;"><br />
<tr><td><br />
<big>HALENUR GÜNGÖRDÜ</big><br/>Medical Student (3rd Year)<br />
</td></tr><br />
<tr><br />
<td><br />
<p>I'm second term student of Fatih University Medical Faculty. This is the first time to attend iGEM competition. In spite of this, we have a great team and our advisors who spend a lot of time for that project. This competition process will be a great advantage for us like working together in solidarity and making idea exchange between us. So this project will broaden our horizon. See you in Holland(o'-'o)//</p><br />
</td><br />
</tr><br />
</table><br />
</td><br />
</tr><br />
<br />
<tr style="height: 200px; "id="7"><br />
<td><br />
<img src="https://static.igem.org/mediawiki/2011/f/ff/Mustafa.jpg"/><br />
</td><br />
<td width="600" style="padding-left: 20px; padding-bottom: 20px;"><br />
<table style="margin-top:0px;"><br />
<tr><td><br />
<big>MUSTAFA SEMİH ELİTOK</big><br/>Medical Student (2nd Year)<br />
</td></tr><br />
<tr><br />
<td><br />
<p>I'm Mustafa, undergraduate student in Fatih University Medical School. First of all, being a part of this team is an honor. I am so impressed of their patience with me. I am definitely a laid-back person, because my point of view to the life is about not taking the things so seriously. I mean seeing the facts on their funny ways, especially when it gets complicated and seems hard. And the recent studies corrects me that not being stressed is healthy, so relax. And I like playing basketball, chess and poking other people.</p><br />
</td><br />
</tr><br />
</table><br />
</td><br />
</tr><br />
<br />
<tr style="height: 200px;" id="8"><br />
<td><br />
<img src="https://static.igem.org/mediawiki/2011/3/30/Secil.jpg"/><br />
</td><br />
<td width="600" style="padding-left: 20px; padding-bottom: 20px;"><br />
<table style="margin-top:0px;"><br />
<tr><td><br />
<big>SEÇİL TAŞHAN</big><br/>Medical Student (2nd Year)<br />
</td></tr><br />
<tr><br />
<td><br />
<p>This is my first year at Fatih University Medical School as a student. Although my department is the Medicine , I have a great interest in Genetics. After I complete my 6-year medical education , I want to maintain my academic education in Genetics. We are the first medical school in Turkey that participates in iGEM Competition. I hope we will maintain our success for next years, too.. I love watching cartoon :)</p><br />
</td><br />
</tr><br />
</table><br />
</td><br />
</tr><br />
<br />
<tr style="height: 200px;" id="9"><br />
<td><br />
<img src="https://static.igem.org/mediawiki/2011/8/81/Ozantahsin1.jpg"/><br />
</td><br />
<td width="600" style="padding-left: 20px; padding-bottom: 20px;"><br />
<table style="margin-top:0px;"><br />
<tr><td><br />
<big>OZAN SAMİ BAYRAK</big><br/>Medical Student (2nd Year)<br />
</td></tr><br />
<tr><br />
<td><br />
<p>I'm Ozan! I'm undergraduate student at Fatih University Medical School. I like genetics and synthetic biology, so I'm a part of this team. I advise you to participate in iGEM if you realy love genetics. In addition, I'm a goalkeeper and football is my life. If I didn't choose to be a doctor, I would be a professional goalkeeper.</p><br />
</td><br />
</tr><br />
</table><br />
</td><br />
</tr><br />
<br />
<tr style="height: 200px;" id="10"><br />
<td><br />
<img src="https://static.igem.org/mediawiki/2011/7/72/Tserap1.jpg"/><br />
</td><br />
<td width="600" style="padding-left: 20px; padding-bottom: 20px;"><br />
<table style="margin-top:0px;"><br />
<tr><td><br />
<big>TÜLİN SERAP YILMAZ</big><br/>Medical Student (2nd Year)<br />
</td></tr><br />
<tr><br />
<td><br />
<p>Hi! I m Tülin Serap Yılmaz. I finished my first year in Fatih University Medical School .I m interested in genetics, surgery and psychiatry. I am too bored learning everything in books; so I took part in Team Genesis . We learn everything by seeing. I wish an enjoying summer full with science for you :)</p><br />
</td><br />
</tr><br />
</table><br />
</td><br />
</tr><br />
<br />
<tr style="height: 200px;" id="11"><br />
<td><br />
<img src="https://static.igem.org/mediawiki/2011/a/a3/Yasincakar.jpg"/><br />
</td><br />
<td width="600" style="padding-left: 20px; padding-bottom: 20px;"><br />
<table style="margin-top:0px;"><br />
<tr><td><br />
<big>YASİN ÇAKAR</big><br/>Medical Student (2nd Year)<br />
</td></tr><br />
<tr><br />
<td><br />
<p>Yasin Çakar.. My first year in medicine school.. Glad to being a part of experimental researches in genetics and being a member of Fatih University iGEM Team. Believing that it's loss if your today is equal to yesterday, tomorrow should be different from today..</p><br />
</td><br />
</tr><br />
</table><br />
</td><br />
</tr><br />
<br />
<tr style="height: 200px;" id="12"><br />
<td><br />
<img src="https://static.igem.org/mediawiki/2011/8/83/Buraksimsek.jpg"/><br />
</td><br />
<td width="600" style="padding-left: 20px; padding-bottom: 20px;"><br />
<table style="margin-top:0px;"><br />
<tr><td><br />
<big>BURAK ŞİMŞEK</big><br/>Computer Engineering Student (4th Year)<br />
</td></tr><br />
<tr><br />
<td><br />
&nbsp;<br />
</td><br />
</tr><br />
</table><br />
</td><br />
</tr><br />
<br />
</table><br />
</center><br />
</div><br />
</div><br />
</div><br />
</div><br />
</div><br />
<br />
<div class="footer-sh-wrapper"><br />
<div class="footer-sh-left"></div><br />
<div class="footer-sh"></div><br />
<div class="footer-sh-right"></div><br />
</div><br />
<div id="footer"><br />
<style><br />
#footer img:hover{<br />
opacity:0.6;<br />
}<br />
</style><br />
<div><br />
<h3 style="color:#BBB;">Sponsors</h3><br />
<img height="100" src="https://static.igem.org/mediawiki/2011/7/72/Tubitak_-_Kopya.png"/><br />
<a href="http://www.sentegen.com"><br />
<img height="100" src="https://static.igem.org/mediawiki/2011/2/2d/Sentegennnn.png"/></a><br />
</div><br />
</div><br />
<div class="copyright-open"> <br />
</div><br />
<div class="copyright"><br />
2011 © Fatih Medical School<br />
</div><br />
</div><br />
<br />
<br />
</body><br />
</html></div>
Metugen
http://2011.igem.org/Team:Fatih_Turkey/Team
Team:Fatih Turkey/Team
2011-09-22T02:49:00Z
<p>Metugen: </p>
<hr />
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<head><br />
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<h2 style="font-family: Verdana, Arial, SunSans-Regular, sans-serif;">Team</h2><br />
<h5 style="font-family: Verdana, Arial, SunSans-Regular, sans-serif;">All Members</h5> <br />
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<tr style="height: 70px; vertical-align: top;"><th colspan="2" style="text-align: center; font-size: 20px; font-weight: bold;">INSTRUCTORS</th></tr><br />
<tr style="height: 200px;" id="15"><br />
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<img src="https://static.igem.org/mediawiki/2011/6/64/Esrahoca.jpg"/><br />
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<big>ESRA GÜNDÜZ</big><br/>(Associate Professor of Medical Genetics)<br />
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<p>Hello everyone! My name is Esra. I’m an Associate Professor of Medical Genetics. This is my third year back in Turkey after staying twelve years in Japan. I mainly work on Cancer Genetics and more specifically on Tumor Suppressor Genes. I do enjoy being with my students and working with them together. iGEM competition has provided us to become closer. I think we share a lot scientifically, socially and friendly together with my students. I also like speaking Japanese and cooking.</p><br />
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<tr style="height: 200px;" id="17"><br />
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<big>KADİR DEMİRCAN</big><br/>(Assistant Professor of Medical Genetics)<br />
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<td>&nbsp;<br />
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<img src="https://static.igem.org/mediawiki/2011/b/b2/Husamettinhoca.jpg"/><br />
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<td width="600" style="padding-left: 20px; padding-bottom: 20px;"><br />
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<big>HÜSAMETTİN ERDAMAR</big><br/>(Assistant Professor of Medical Biochemistry)<br />
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<td>&nbsp;</td><br />
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<tr style="height: 70px;vertical-align: top;"><th colspan="2" style="text-align: center; font-size: 20px; font-weight: bold;">ADVISORS</th></tr><br />
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<tr style="height: 200px;" id="13"><br />
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<img src="https://static.igem.org/mediawiki/2011/c/c1/Cihantastan.jpg"/><br />
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<big>CİHAN TAŞTAN</big><br/>(Ph.D Student on Tumor Immunology at Sackler Institue of New York University)<br />
</td></tr><br />
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<td><br />
<p> I am graduate student for Ph.D. on Tumor Immunology at Sackler Institue of New York University. I have participated iGEM competition several times. I have taken parts in the competition as a student member of METU-Gene 2009 (Silver), METU-Turkey Software 2010 (Silver) and METU-Turkey 2010 (Gold). Now, I have been advisor of Fatih University for couple of months in order to start integration of other universities of Turkey into iGEM and to prepare an environment for establisihing and spreading of new synthetic biology labs through Turkey.</p><br />
</td><br />
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<tr style="height: 200px;" id="14"><br />
<td><br />
<img src="https://static.igem.org/mediawiki/2011/e/e3/Muradiye.jpg"/><br />
</td><br />
<td width="600" style="padding-left: 20px; padding-bottom: 20px;"><br />
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<big>MURADİYE ACAR</big><br/>(Ph.D Student on Mecdical Genetics)<br />
</td></tr><br />
<tr><br />
<td><br />
&nbsp; </td><br />
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</td><br />
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<tr style="height: 20px;vertical-align: top;"><th colspan="2" style="text-align: center; font-size: 20px; font-weight: bold;">ATTRIBUTION</th></tr><br />
<br />
<div><tr style="height:100px;"><td colspan="2"> Canvas Town is a proud product of Fatih Turkey group where all work presented on this wiki is the sole work of the iGEM team members listed below.<br />
All project executions have been performed by medical school team members through the summer. They have also performed all activities of Human practice.<br />
Additionally all the logo, illustrations, cartoons, other visual media and web design has been created by the design team in the group.<br />
</td></tr></div><br />
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<tr style="height: 70px;vertical-align: top;"><th colspan="2" style="text-align: center; font-size: 20px; font-weight: bold;">STUDENTS</th></tr><br />
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<tr style="height: 200px;" id="1"><br />
<td><br />
<img src="https://static.igem.org/mediawiki/2011/0/0a/Alperen.jpg"/><br />
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<td width="600" style="padding-left: 20px; padding-bottom: 20px;"><br />
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<big>ALPEREN GÜVER</big><br/>Medical Student (2nd Year)<br />
</td></tr><br />
<tr><br />
<td><br />
<p>I am a first term student of Fatih University Medical Faculty and I will finish first year on July. When I finish my faculty, I want to be a clinician. I like playing tennis and taking some photos.</p><br />
</td><br />
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</td><br />
</tr><br />
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<tr style="height: 200px;" id="2"><br />
<td><br />
<img src="https://static.igem.org/mediawiki/2011/f/f7/Betul.jpg"/><br />
</td><br />
<td width="600" style="padding-left: 20px; padding-bottom: 20px;"><br />
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<big>BETÜL GÜNBEY</big><br/>Medical Student (2nd Year)<br />
</td></tr><br />
<tr><br />
<td><br />
<p>I’m a first year student in medical faculty. Also this is my first year in iGEM, I’m interested in clinical medicine; but also, I always like genetics and working in the lab. I think iGEM is a perfect way to learn synthetic biology and teamwork for a student. I like playing guitar. I’m sure our team will be very successful.</p><br />
</td><br />
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</td><br />
</tr><br />
<br />
<tr style="height: 200px;" id="3"><br />
<td><br />
<img src="https://static.igem.org/mediawiki/2011/0/0f/Hasanyasin.jpg"/><br />
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<td width="600" style="padding-left: 20px; padding-bottom: 20px;"><br />
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<big>HASAN YASİN SOYLU</big><br/>Medical Student (2nd Year)<br />
</td></tr><br />
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<td><br />
<p>I'm happy to be a part of the team. There is nothing to say about the past time; but I'm here for the future.<br />
"Bismillah is the beginning of every good thing. So, let's say Bismillah!.."</p><br />
</td><br />
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<tr style="height: 200px;" id="4"><br />
<td><br />
<img src="https://static.igem.org/mediawiki/2011/7/7a/Team-fazilet.jpg"/><br />
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<big>FAZİLET GÜLER</big><br/>Medical Student (3rd Year)<br />
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<td><br />
<p>I finished my second year in medical school on June. I'll be a doctor but I don't want to be a clinician. Thus, genetics and synthetic biology are treasure for me:) This is my first year in iGEM. I'm interested in physics and computer science. I like traveling and hiking. Also, I enjoy riding a roller coaster!</p><br />
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<tr style="height: 200px;" id="5"><br />
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<img src="https://static.igem.org/mediawiki/2011/9/95/Kenangzl.jpg"/><br />
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<td width="600" style="padding-left: 20px; padding-bottom: 20px;"><br />
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<big>KENAN OĞUZ GÜZEL</big><br/>Cartoonist<br />
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<td><br />
&nbsp;<br />
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</table><br />
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<tr style="height: 200px;" id="6"><br />
<td><br />
<img src="https://static.igem.org/mediawiki/2011/d/d0/Hale.jpg"/><br />
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<td width="600" style="padding-left: 20px; padding-bottom: 20px;"><br />
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<big>HALENUR GÜNGÖRDÜ</big><br/>Medical Student (3rd Year)<br />
</td></tr><br />
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<td><br />
<p>I'm second term student of Fatih University Medical Faculty. This is the first time to attend iGEM competition. In spite of this, we have a great team and our advisors who spend a lot of time for that project. This competition process will be a great advantage for us like working together in solidarity and making idea exchange between us. So this project will broaden our horizon. See you in Holland(o'-'o)//</p><br />
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<tr style="height: 200px; "id="7"><br />
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<img src="https://static.igem.org/mediawiki/2011/f/ff/Mustafa.jpg"/><br />
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<big>MUSTAFA SEMİH ELİTOK</big><br/>Medical Student (2nd Year)<br />
</td></tr><br />
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<td><br />
<p>I'm Mustafa, undergraduate student in Fatih University Medical School. First of all, being a part of this team is an honor. I am so impressed of their patience with me. I am definitely a laid-back person, because my point of view to the life is about not taking the things so seriously. I mean seeing the facts on their funny ways, especially when it gets complicated and seems hard. And the recent studies corrects me that not being stressed is healthy, so relax. And I like playing basketball, chess and poking other people.</p><br />
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<tr style="height: 200px;" id="8"><br />
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<img src="https://static.igem.org/mediawiki/2011/3/30/Secil.jpg"/><br />
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<big>SEÇİL TAŞHAN</big><br/>Medical Student (2nd Year)<br />
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<td><br />
<p>This is my first year at Fatih University Medical School as a student. Although my department is the Medicine , I have a great interest in Genetics. After I complete my 6-year medical education , I want to maintain my academic education in Genetics. We are the first medical school in Turkey that participates in iGEM Competition. I hope we will maintain our success for next years, too.. I love watching cartoon :)</p><br />
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<tr style="height: 200px;" id="9"><br />
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<img src="https://static.igem.org/mediawiki/2011/8/81/Ozantahsin1.jpg"/><br />
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<big>OZAN SAMİ BAYRAK</big><br/>Medical Student (2nd Year)<br />
</td></tr><br />
<tr><br />
<td><br />
<p>I'm Ozan! I'm undergraduate student at Fatih University Medical School. I like genetics and synthetic biology, so I'm a part of this team. I advise you to participate in iGEM if you realy love genetics. In addition, I'm a goalkeeper and football is my life. If I didn't choose to be a doctor, I would be a professional goalkeeper.</p><br />
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<tr style="height: 200px;" id="10"><br />
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<img src="https://static.igem.org/mediawiki/2011/7/72/Tserap1.jpg"/><br />
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<big>TÜLİN SERAP YILMAZ</big><br/>Medical Student (2nd Year)<br />
</td></tr><br />
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<td><br />
<p>Hi! I m Tülin Serap Yılmaz. I finished my first year in Fatih University Medical School .I m interested in genetics, surgery and psychiatry. I am too bored learning everything in books; so I took part in Team Genesis . We learn everything by seeing. I wish an enjoying summer full with science for you :)</p><br />
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<tr style="height: 200px;" id="11"><br />
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<img src="https://static.igem.org/mediawiki/2011/a/a3/Yasincakar.jpg"/><br />
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<big>YASİN ÇAKAR</big><br/>Medical Student (2nd Year)<br />
</td></tr><br />
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<td><br />
<p>Yasin Çakar.. My first year in medicine school.. Glad to being a part of experimental researches in genetics and being a member of Fatih University iGEM Team. Believing that it's loss if your today is equal to yesterday, tomorrow should be different from today..</p><br />
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<img src="https://static.igem.org/mediawiki/2011/8/83/Buraksimsek.jpg"/><br />
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<h2 style="font-family: Verdana, Arial, SunSans-Regular, sans-serif;">Project</h2><br />
<h5 style="font-family: Verdana, Arial, SunSans-Regular, sans-serif;">Experiments</h5> <br />
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<div style="font-size:15px; color: #333;"><br />
A)EXPERIMENTS<br />
<p style="padding-left: 30px;">1)<a href="#1">Disc experiments 1</a></p><br />
<p style="padding-left: 30px;">2)<a href="#2">Disc experiments 2</a></p><br />
<p style="padding-left: 30px;">3)<a href="#3">Sterilization period of Supernatant</a></p><br />
<p style="padding-left: 30px;">4)<a href="#4">The Suicide Experiment Of E. COLI</a></p><br />
<p style="padding-left: 30px;">5)<a href="#5">The Effect Of Feton Reant Solution On The Biofilm</a></p><br />
<p style="padding-left: 30px;">6)<a href="#6">The Difference between Colonies Containing and Not Containing Gene Parts of Reflectin Protein</a></p><br />
<p style="padding-left: 30px;">7)<a href="#7">Experiment of Supernatant</a></p><br />
<p style="padding-left: 30px;">8)<a href="#8">Experiment&nbsp;&nbsp;of liquid culture</a></p><br />
B)<a href="#B">CONFIRMATION</a><br />
<br><br />
C)<a href="#C">CHARACTERIZATION</a><br />
<br />
&nbsp;<br />
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<br />
<span id="1"><strong><h1>Disc Experiment 1</h1></strong></span><br />
<p>We prepared an experiment like the disc experiments methods used to see antibiotic effectiveness, in order to see the effect of B. Subtillis with LALF on E. Coli.</p><br />
<p>By adding E. Coli on Bacillus Subtilis;</p><br />
<p><strong>Plate A1);</strong> We put E. Coli on the B. Subtilis with LALF (K541915) biofilm. For his purpose we first prepared acorn starch with liquid LB medium and added 100 ul B. Subtilis liquid medium as a point over it. For a qualified biofilm we incubated it for 24 hours at 37˚C.</p><br />
<p>Later we added 10 uL E. Coli with RFP as a single point and again incubated it.</p><br />
<p><strong>Plate A2);</strong> In order to understand whether LALF protein or another factor killed E. Coli on the Bacillus biofilm we prepared the same contrivance with a B. subtilis biofilm that doesn’t produce LALF protein or doesn’t have antibiotic resistance. So we could understand if the B. Subtilis biofilm itself not LALF, inhibited the growth of E. Coli.</p><br />
<p><strong>Plate A3);</strong>At this contrivancethe B. Subtilis we used for biofilm didn’t includeLALF protein but had antibiotic resistance. Why we didi this is we knew that there would be a color difference between Plate A1 and A2 but we woluld like to know if the antibiotic would prevent E. Coli with RFP from having color.</p><br />
<p><strong>Plate B1);</strong> We spreaded B. Subtilis with LALF over a normal lb medium. We put E. Coli as a single point while the Bacillus spread is fresh. We aimed to see if B. Subtilis could kill E. Coli without forming a biofilm.</p><br />
<p><strong>Plate B2);</strong> We prepared this plate as a control plate of Plate C. For thşs we prepared the same contrivance with only B Subtilis that doesn’t produce LALF protein or doesn’t have antibiotic resistance in order to be sure that if the EColi of the plate B1 does not grow the reason for this is LALF protein.</p><br />
<p><strong>Plate B3);</strong> In this contrivance we spread B. Subtilis that doesn’t produce LALAF protein but has antibiotic resistance and added E.Coli with RFP as a single drop over it.</p><br />
<p>By adding E. Coli over B. Subtilis ;</p><br />
<p><strong>Plate C1);</strong> We added 100 ul liquid culture of B. Subtilis with LAFL (k541915), over the normal lg medium applied with E. Coli with RFP,as a single drop.</p><br />
<p><strong>Plate C2);</strong> Over the fresh spread of E. Coli, 100 ul of liquid culture of B. Subtiis not producing LALF or doesn’t have antibiotic resistance as a single drop.</p><br />
<p><strong>Plate C3);</strong> At this contrivance we added B. Subtilis that doesn’t produce LALF but has antibiotic resistance over the fresh spread of E. Coli with RFP likewise. We aimed to see the affect of antibiotic resistance over E. Coli with RFP ,by using B. subtilis with and without antibiotic resistance.</p><br />
<p><strong>Plate D1);</strong> We added supernatant of B. Subtilis with LALF as a single drop over fresh spread of E. Coli with RFP. Because at the beginning of the LALF part of B. Subtilis there was a throwing out signal and we expected death mostly here.</p><br />
<p><strong>Plate D2);</strong> At this plate we applied 100 ul of supernatant of B: subtilis not producing LALF protein and not having antibiotic resistance over the fresh spread of E. Coli as a single drop.</p><br />
<p><strong>Plate D3);</strong> At this plate we applied 100 ul of supernatant of B: subtilis not producing LALF protein and but that has antibiotic resistance over the fresh spread of E. Coli as a single drop.</p><br />
<p><img src="https://static.igem.org/mediawiki/2011/d/d1/Sss1.png"></p><br />
<p>Plates B2 and B1 (Respectively)</p><br />
<p><img src="https://static.igem.org/mediawiki/igem.org/f/fd/Sss2.png"></p><br />
<p>Plates C1 and C2 (Respectively)</p><br />
<p><img src="https://static.igem.org/mediawiki/2011/8/85/Sss3.png" ></p><br />
<p>Plates D2 and D1</p><br />
<p><strong><span style="text-decoration: underline;"><br /><br />
</span></strong></p><br />
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<p><strong><span id="2"><h1>DISC EXPERIMENT 2</h1></span></strong></p><br />
<p>We have prevented E.coli growth in our experiment. We took antibiotic disks which are used in hospitals to identify which type of antibiotic is the most effective against to unknown bacteria.</p><br />
<p>We put antibiotic disks onto spreads of E.coli which produces RFP protein and has the chloramphenicol resistance. Then, we added different amounts of supernatant.</p><br />
<p>We used supernatant with LALF and without LALF of B.subtilis. We centrifuged LB broth culture which was incubated for 12 hours at 37°C at 7000 rpm for 5 minutes. It is filtered with 0,2 filter, afterwards.</p><br />
<p><img src="https://static.igem.org/mediawiki/2011/f/f9/IMG_0331.JPG"></p><br />
<p>E.coli with RFP was spread on both of two plates. 40ul, 80ul, 120ul and 160ul supernatant of B.subtilis which do not include LALF protein was added into left plate. Then 40ul, 80ul, 120ul and 160 supernatant of B.subtilis which include LALF protein was added into right plate .We got results which support other experiments.</p><br />
<p><img src="https://static.igem.org/mediawiki/2011/1/1f/IMG_0335.JPG"></p><br />
<p>&nbsp;</p><br />
<p><img src="https://static.igem.org/mediawiki/2011/a/a6/IMG_0339.JPG"></p><br />
<p>Supernatants with and without LALF protein was put onto 50 ul spread of E.coli with RFP in declared amount at the picture .Again we got results which is overlapped other our experiments.</p><br />
<p>The reason of making this experiment with antibiotic and without antibiotic plate was if antibiotic effects LALF or not?</p><br />
<p><strong><br /><br />
</strong></p><br />
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<p><strong><h1><span id"3">Sterilization period of Supernatant</span></h1></strong></p><br />
<p>We have deduced that supernatant of B.subtilis that produces LALF sterilize the surfaces that LALF had applied on. Thus, we wanted to test how long this sterilization exists. To do this, supernatants with and without LALF waited in +4 ⁰C for 24 and 36 hours. Then, we tested their ability to sterilize.</p><br />
<p><img src="https://2011.igem.org/File:Mustafa1.JPG"></p><br />
<p>Equal amount of E.coli with RFP sequence were spread into the fresh plates Then, 100 ul supernatant with and without was added on plates. Note that no colony grew on where supernatant that contents LALF was added after both 24 and 36 hours. (the plates on the right)</p><br />
<p><img src="https://static.igem.org/mediawiki/2011/1/10/Mustafa2.JPG"></p><br />
<p>To understand better how much effective our protein is, we measured the diameter of the no growth zone. In 24 hour waited plate, diameter was 2,5 cm; on the other hand, it was measured 1,7 cm in 36 hour waited plate.</p><br />
<p>&nbsp;</p><br />
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<h1><strong><span id="4">THE SUICIDE EXPERIMENT OF E. COLI</span></strong></h1><br />
<p><em>Our K541545 part contains IPTG promoter and the gene part of LALF protein on a backbone which has resistance to chloramphenicol. We transported this part to E. coli for testing whether E. coli would kill itself or not.</em></p><br />
<p><strong>Experiments of plates with and without IPTG<em> </em></strong></p><br />
<p>Plate A1: We streaked E coli culture which has K541545 on this plate and the plate includes IPTG and Chloramphenicol.</p><br />
<p>Plate A2: We streaked E coli culture which has K541545 on this plate and the plate includes just Chloramphenicol.</p><br />
<p>Plate B1: This plate does not have IPTG and we streaked E.coli culture which does not have IPTG promoter.</p><br />
<p>PlateB2: This plate has IPTG and we streaked E coli culture which has IPTG promoter.</p><br />
<p>We prepared the B1 and B2 plates for testing toxic effect of IPTG. Because we wanted to be sure that the only factor affecting growth of E coli is LALF protein.</p><br />
<p>&nbsp;</p><br />
<p><strong> </strong></p><br />
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<p><strong><span id="5"><h1>THE EFFECT OF FENTON REAGENT SOLUTION ON THE BIOFILM</h1></span></strong></p><br />
<p>Fenton Reagent solution is a solution that can kill the B. Subtilis spors and bacteria. It kills the spors by oxidizing with its Cu ions.</p><br />
<p>We prepared this solution in two ways one with the hydrogen peroxide (OFR) according to the literature and the other with distilized water (DFR). In our experiments we learned which of these two solutions is more effective on E. Coli, B. Subtilis or the the spores of B. Subtilis and degree of its effectiveness.</p><br />
<p>The aim of this experiment is seeing the effects of the fenton reagen solution on the B. Subtilis and E. Coli on the biofilms.</p><br />
<p>We prepared Corn Strach Lb Agar for a more qualified biofilm. In order to understand the death in the plates we added a small amount to the liquid culture.</p><br />
<p>Plate 1: On this plate OFR is added on the B. Subtilis biofilm</p><br />
<p>Plate 2: We formed another B. Subtilis biofilm and added same amount of DFR on it.</p><br />
<p>Plate 3: On this contrivance one drop of E. Coli with RFP on the B. Subtilis biofilm and added same amount of DFR on it</p><br />
<p>Plate 4: On this contrivance one drop of E. Coli with RFP on the B. Subtilis biofilm and added same amount of OFR on it</p><br />
<p>Plate 5: this plate prepared as a control group of 3<sup>rd</sup> and 4<sup>th</sup> plates. On this contrivance one drop of E. Coli with RFP on the B. Subtilis biofilm but any kind of fenton reagent solutions didn’t add on it.</p><br />
<p>RESULT: After adding the FR solutions on all of plates, we measured their OD values in the end of this experiment we measured again and saw that the 1<sup>st</sup>, 2<sup>nd</sup>, 3<sup>rd</sup>, 4<sup>th</sup> plates OD values didn’t change. But the the OD value of 5<sup>th</sup> plate increased. 5<sup>th</sup> plate is the control group.</p><br />
<p>&nbsp;</p><br />
<p>&nbsp;</p><br />
<p><img src="https://static.igem.org/mediawiki/igem.org/6/6e/S1.png"></p><br />
<p>Figur2: plate 3 and plate 4</p><br />
<p><img src="https://static.igem.org/mediawiki/2011/4/40/S2.png"></p><br />
<p><img src="https://static.igem.org/mediawiki/2011/a/af/S3.png"></p><br />
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<p><strong><span id="6"></span></strong></p><h1><strong>The Difference between Colonies Containing and Not Containing Gene Parts of Reflectin Protein</strong></h1><p></p><br />
<p>We transfered the plasmid which includes the gene part of reflectin protein (j04450 shuttle, j04500 backbone, and 1006) to E. Coli and we saw that there is a difference between reflectin including colonies and the colonies which does not include reflectin protein.</p><br />
<p>We saw that the plate which does not include reflectin protein is normally transparent. But the other plate which includes reflectin protein seemed white. And this is showing us that our plasmid is working and the protein is produced by E. coli.<br><br />
Figure-1: the difference of colonies is showing clearly, the colonies at the right side are seemed white and the colonies at the left side are seemed transparent.</p><br />
<p><img src="https://static.igem.org/mediawiki/igem.org/3/34/Aaa1.png"></p><br />
<p><img src="https://static.igem.org/mediawiki/igem.org/1/14/Aaa2.png"></p><br />
<p>Additionally, the colonies are looked into by microscope. The colony which is including reflectin has some transparent points. But the control group hasn’t these points. We are thinking that because of reflectin protein there are the points in these colonies.</p><br />
<p>&nbsp;</p><br />
<p><img src="https://static.igem.org/mediawiki/igem.org/0/09/Aaa3.png"><br><img src="https://static.igem.org/mediawiki/igem.org/9/9a/Aaa4.png"></p><br />
<p>Figure-4: Image of the colonies which are include reflectin protein by microscope it’s clearly showing that the transparent points in the colonies</p><br />
<p>&nbsp;</p><br />
<p>Figure-6: Image of the control group which is not including the 1006 gene part and reflectin protein and it can clearly showing the difference from the colonies which include reflectin protein. There aren’t the transparent points here.</p><br />
<p><img src="https://static.igem.org/mediawiki/igem.org/0/0f/Aaa5.png"></p><br />
<p>Consequently, it’s confirmed that the 1006 gene part is producing the reflectin protein</p><br />
<p>&nbsp;</p><br />
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<div><br />
<p></p><h1><strong><span id="7">Experiment of Supernatant</span></strong></h1><p></p><br />
</div><br />
<p>We wanted to investigate to effects of supernatant includes LALF protein to E.coli in this experiment.</p><br />
<p>Therefore we put 8 ul LB broth into falcons.then we added them E.coli which has rfp and incubated for 12 hours in 37⁰C .We put pure E.coli into only one falcon for control.We added 1ul,10ul and 100ul supernatant with LALF and without LALF.</p><br />
<p>We diluated them 7 times 2-4-8-12hours later then we spreaded 10ul to plates which has 1/0,5 l Cloramphenicol.We did santrifuge liquid culture which incubated for 12 hours at 37⁰C at 7000 rpm for 5 minutes because of preparing supernant with LALF and without LALF.Then we passed them from the 0,2 filter.</p><br />
<p>Plates were spreaded after 2 hours later:</p><br />
<p><img src="https://static.igem.org/mediawiki/igem.org/5/5f/2saat.jpg"></p><br />
<p>1a)We added 1ul supernatant includes LALF into left plate and 1ul supernatant without LALF into right plate</p><br />
<p><img src="https://static.igem.org/mediawiki/igem.org/1/12/2saat_b.jpg"></p><br />
<p>1b)We added 10 ul supernatant includes LALF into left plate and 10 ul supernatant without LALF into right plate</p><br />
<p><img src="https://static.igem.org/mediawiki/igem.org/d/db/2_saat_c.jpg"></p><br />
<p>1c)We added 100ul supernatant includes LALF into left plate and 100ul supernatant without LALF into right plate</p><br />
<p>Plates were spreaded after 4 hours later;</p><br />
<p><img src="https://static.igem.org/mediawiki/igem.org/9/9d/4saat_a.jpg"></p><br />
<p>2a)We added 1ul supernatant includes LALF into left plate and 1ul supernatant without LALF into right plate.</p><br />
<p><img src="https://static.igem.org/mediawiki/igem.org/9/9a/4saat_b.jpg"></p><br />
<p>2b)We added 100ul supernatant includes LALF into left plate and 100 ul supernatant without LALF into right plate.</p><br />
<p>Plates were spreaded after 8 hours later;</p><br />
<p><img src="https://static.igem.org/mediawiki/igem.org/e/e7/8_saat_a.jpg"></p><br />
<p>3a)We added 1ul supernatant includes LALF into left plate and 1ul suoernatant without LALF into right plate.</p><br />
<p><img src="https://static.igem.org/mediawiki/igem.org/c/ca/8_saat_b.jpg"></p><br />
<p>3b)We added 10 ul supernatant includes LALF into left plate and 10 ul supernatant without LALF into right plate.</p><br />
<p><img src="https://static.igem.org/mediawiki/igem.org/d/de/8_saat_c.jpg"></p><br />
<p>3c) We added 100ul supernatant includes LALF into left plate and 100 ul supernatant without LALF into right plate.</p><br />
<p>Plates were spreaded after 12 hours later;</p><br />
<p><img src="https://static.igem.org/mediawiki/igem.org/1/19/12_saat_a.jpg"></p><br />
<p>4a) We added 1ul supernatant includes LALF into left plate and 1ul suoernatant without LALF into right plate.</p><br />
<p><img src="https://static.igem.org/mediawiki/igem.org/2/2b/12_saat_b.jpg"></p><br />
<p>4b)We added 10 ul supernatant includes LALF into left plate and 10 ul supernatant without LALF into right plate.</p><br />
<p><img src="https://static.igem.org/mediawiki/igem.org/f/fc/12_saat_c.jpg"></p><br />
<p>4c)We added 100ul supernatant includes LALF into left plate and 100 ul supernatant without LALF into right plate.</p><br />
<p><em><br><br />
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<h3><strong><span id="8"><h1>Experiment of liquid culture</h1></span></strong></h3><br><br />
&nbsp;&nbsp;&nbsp;We wanted to see the effects of supernatant of B.subtilis which produces LALF in order to stop e.coli with RFP growth in liquid culture.<br><br />
&nbsp;&nbsp;&nbsp;We put 8 ml LB broth in falcons.<br><br />
&nbsp;&nbsp;&nbsp;We planned 3 control groups.These are:<br />
<ul><br />
<li>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Control group 1:Includes only 5ul e.coli which waited for 12 hours in 37⁰C. These can synthesize RFP.</li><br />
<li>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Control group 2:Includes only 5 ul b.subtilis which produces LALF. They waited for 12 hours in 37⁰C.</li><br />
<li>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Control group 3:Includes only 5ul b.subtilis which do not produce LALF. They waited for 12 hours in 37⁰C.</li><br />
</ul><br />
&nbsp;&nbsp;&nbsp;First of all, we put 5ul e.coli which has RFP into 8 falcons as experimental groups. We waited experimental falcons for 12 hours in 37⁰C . Then, we added 1ul,10ul,100ul and 1000ul liquid culture with LALF and liquid culture without LALF. After this, we incubated them in 37⁰C and measured their OD value 2-4-8-12 hours later in 405 nm. Their results are as below:<br><br />
<img src="https://static.igem.org/mediawiki/2011/e/ee/Graphicexp1.jpg"><br><br />
Graph1 : Absorbance(OD405) value difference through time of AntiLPS-LPS binding with 1 ul LALF<br><br />
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<img src="https://static.igem.org/mediawiki/2011/f/fb/Graphicexp2.jpg"><br><br />
Graph2 : Absorbance(OD405) value difference through time of AntiLPS-LPS binding with 10 ul LALF<br><br />
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<img src="https://static.igem.org/mediawiki/2011/f/f1/Graphicexp3.jpg"><br><br />
Graph3 : Absorbance(OD405) value difference through time of AntiLPS-LPS binding with 100 ul LALF<br><br />
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<img src="https://static.igem.org/mediawiki/2011/b/b4/Graphicexp4.jpg"><br><br />
Graph4 : Absorbance(OD405) value difference through time of AntiLPS-LPS binding with 1000 ul LALF<br><br />
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<br><hr><span id="B"><br />
<h1><strong>CONFIRMATION</strong></h1><br />
<img src="https://static.igem.org/mediawiki/igem.org/d/db/M1.jpg"></span><br><br />
1: This part includes a gram positive promoter, an RBS sequence and SacB signal peptide sequence to synthesize the protein outside of the cell. This image shows that indicated part is considered as confirmed.<br />
<hr><br />
<img src="https://static.igem.org/mediawiki/2011/c/cd/M2.jpg"><br><br />
2:This part includes a gram positive promoter, an RBS sequence and LipA signal peptide sequence to synthesize the protein outside of the cell. This image shows that indicated part is considered as confirmed.<br />
<hr><br />
<img src="https://static.igem.org/mediawiki/2011/7/77/M3.jpg"><br><br />
3: This part includes a constitutive promoter, an RBS sequence and Tat signal peptide sequence to synthesize the protein outside of the cell. This image shows that indicated part is considered as confirmed.<br />
<hr><br />
<img src="https://static.igem.org/mediawiki/2011/5/5d/M4.jpg"><br><br />
4:This part includes a IPTG inducible promoter, an RBS sequence and Tat signal peptide sequence to synthesize the protein outside of the cell. This image shows that indicated part is considered as confirmed.<br />
<hr><br />
<img src="https://static.igem.org/mediawiki/2011/4/49/M5.jpg"><br><br />
5:Our “LALF (limulus anti-lipopolysaccharide factor)” protein allows stopping any kind of gram negative bacteria growth by binding their cell wall material, LPS. This image shows that indicated part is considered as confirmed.<br />
<hr><br />
<img src="https://static.igem.org/mediawiki/2011/6/6c/M6.jpg"><br><br />
6:Our “reflectin” protein has the ability to reflect the light by changing its wavelength, thus its color. In our project, we planned to use this protein as an indicator on E.coli whether our LALF protein works properly or not. This image shows that indicated part is considered as confirmed.<br />
<hr><br />
<img src="https://static.igem.org/mediawiki/2011/6/6c/M7.jpg"><br><br />
8:This part is a well-studied gram positive promoter. We planned to use this gene in the case of our signal peptide sequences do not work. When the protein, which is attached to this gene, is produced, we planned to blow up the bacteria; therefore synthesized protein would be in supernatant. This image shows that indicated part is considered as confirmed.<br />
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<hr><br />
<img src="https://static.igem.org/mediawiki/igem.org/0/07/M8.jpg"><br><br />
500: To perform cloning perfectly, we used pSB1C3 in E.coli. This vector is also the main vector for our parts that works only in E.coli. All of our parts are also inserted into this vector; because it is declared that all parts must be sent to Registry in pSB1C3. This image shows that this part is confirmed.<br />
<br />
<hr><br />
<img src="https://static.igem.org/mediawiki/2011/c/c0/Ms4.png"><br><br />
501: To perform ligation procedure appropriately, we designed an alternative part for all of our material parts. We inserted our promoters and proteins into pSB1C3 in order to have the alternative part that possesses different antibiotic resistance, comparing to original vectors of the parts. Moreover, it is wanted that all parts must be sent to Registry in pSB1C3. This part includes a gram positive promoter, RBS sequence and SacB signal peptide sequence. In the image, it can be seen that this part is confirmed.<br />
<br />
<hr><br />
<img src="https://static.igem.org/mediawiki/2011/6/61/M10.jpg"><br><br />
502: To perform ligation procedure appropriately, we designed an alternative part for all of our material parts. We inserted our promoters and proteins into pSB1C3 in order to have the alternative part that possesses different antibiotic resistance, comparing to original vectors of the parts. Moreover, it is wanted that all parts must be sent to Registry in pSB1C3. This part includes a gram positive promoter, RBS sequence and LipA signal peptide sequence. In the image, it can be seen that this part is confirmed.<br />
<br />
<hr><br />
<img src="https://static.igem.org/mediawiki/2011/4/48/M11.jpg"><br><br />
505:To perform ligation procedure appropriately, we designed an alternative part for all of our material parts. We inserted our promoters and proteins into pSB1C3 in order to have the alternative part that possesses different antibiotic resistance, comparing to original vectors of the parts. Moreover, it is wanted that all parts must be sent to Registry in pSB1C3. This part includes the sequence of our LALF protein. In the image, it can be seen that this part is confirmed.<br />
<br />
<hr><br />
<img src="https://static.igem.org/mediawiki/2011/a/aa/M12.jpg"><br><br />
506:To perform ligation procedure appropriately, we designed an alternative part for all of our material parts. We inserted our promoters and proteins into pSB1C3 in order to have the alternative part that possesses different antibiotic resistance, comparing to original vectors of the parts. Moreover, it is wanted that all parts must be sent to Registry in pSB1C3. This part includes the sequence of our reflectin protein. In the image, it can be seen that this part is confirmed.<br />
<br />
<hr><br />
<img src="https://static.igem.org/mediawiki/2011/2/25/M13.jpg"><br><br />
800: In our project, we planned to use gram positive bacteria to synthesize our LALF protein in order to stop gram negative growth. On the other hand, to identify effectively whether our protein works or not, we decided to use reflectinprotein and to produce it from both of our bacteria; B.subtilis and E.coli. Thus, we determined to use a shuttle vector that works both bacteria kinds. This vector has ampicillin resistance for E.coli and chloramphenicol resistance for B.subtilis. In the image, it can be seen that this part is confirmed.<br />
<br />
<hr><br />
<img src="https://static.igem.org/mediawiki/2011/a/a0/Ms3.png"><br><br />
900:In our project, we planned to use gram positive bacteria to synthesize our LALF protein in order to stop gram negative growth. On the other hand, to identify effectively whether our protein works or not, we decided to use reflectin protein and to produce it from both of our bacteria; B.subtilis and E.coli. Thus, we determined to use a shuttle vector that works both bacteria kinds. This vector has chloramphenicol resistance for both of bacteria kinds. This part also includes an RFP sequence. In the image, it can be seen that this part is confirmed.<br />
<hr><br />
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<img src="https://static.igem.org/mediawiki/igem.org/a/a5/M16.jpg"><br><br />
545: This part is designed; because it is wanted that all parts must be sent to headquarters in pSB1C3 vector. It possesses a promoter that works in gram negative bacteria, Tat signal sequence in order to synthesize the protein outside and LALF protein gene. In this image, indicated part is considered as confirmed.<br />
<hr><br />
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<img src="https://static.igem.org/mediawiki/2011/6/64/Ms2.png"><br><br />
815: In order to stop gram negative bacteria growth, our LALF protein must be synthesized by another type of bacteria, gram positive. Thus, we designed a special gene that works in only gram positive bacteria by inserting a promoter specialized for gram positive. Also, SacB signal sequence that allows us synthesizing the protein outside and LALF protein gene is included.All components are inserted into 800 that isdesigned for gram positive. In this image, indicated part is considered as confirmed<br />
<hr><br />
<br />
<img src="https://static.igem.org/mediawiki/2011/6/6c/Ms1.png"><br><br />
915: In order to stop gram negative bacteria growth, our LALF protein must be synthesized by another type of bacteria, gram positive. Thus, we designed a special gene that works in only gram positive bacteria by inserting a promoter specialized for gram positive. Also, SacB signal sequence that allows us synthesizing the protein outside and LALF protein gene is included.All components are inserted into 900 that is designed for gram positive. In this image, indicated part is considered as confirmed.<br />
<hr><br />
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<img src="https://static.igem.org/mediawiki/2011/5/54/M18.jpg"><br><br />
925:In order to stop gram negative bacteria growth, our LALF protein must be synthesized by another type of bacteria, gram positive. Thus, we designed a special gene that works in only gram positive bacteria by inserting a promoter specialized for gram positive. Also, LipA signal sequence that allows us synthesizing the protein outside and LALF protein gene is included.All components are inserted into 900 that is designed for gram positive. In this image, indicated part is considered as confirmed.<br />
<br />
<hr><br />
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<img src="https://static.igem.org/mediawiki/2011/3/30/M19.jpg"><br><br />
516: This part is designed; because all parts must be sent to headquarters in pSB1C3 vector. In this image, indicated part is considered as confirmed.<br />
<br />
<hr><br />
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<img src="https://static.igem.org/mediawiki/2011/5/5c/M20.jpg"><br><br />
526: This part is designed; because all parts must be sent to headquarters in pSB1C3 vector.In this image, indicated part is considered as confirmed.<br />
<br />
<hr><br />
<br />
<img src="https://static.igem.org/mediawiki/2011/d/d9/M21.jpg"><br><br />
536:In our experiments, we aimed to indicate whether our LALF protein works or not, we used an alternative indicator protein, reflectin. This part is designed to work in gram negative E.coli. A constitutive promoter, a Tat signal sequence that allows synthesizing the protein outside and reflectin protein gene is included in pSB1C3. In this image, indicated part is considered as confirmed.<br />
<br />
<hr><br />
<br />
<img src="https://static.igem.org/mediawiki/2011/3/3b/M22.jpg"><br><br />
546:In our experiments, we aimed to indicate whether our LALF protein works or not, we used an alternative indicator protein, reflectin. This part is designed to work in gram negative E.coli. An IPTG inducible promoter, a Tat signal sequence that allows synthesizing the protein outside and reflectin protein gene is included in pSB1C3. In this image, indicated part is considered as confirmed.<br />
<br />
<hr><br />
<br />
<img src="https://static.igem.org/mediawiki/igem.org/8/85/M23.jpg"><br><br />
596:In our experiments, we aimed to indicate whether our LALF protein works or not, we used an alternative indicator protein, reflectin. We designed an alternative part in the case that our signal sequences do not work. This part includes a promoter that works in only gram negative and reflectin protein gene. All components are inserted into pSB1C3. In this image, indicated part is considered as confirmed.<br />
<br />
<br />
<br />
<br />
<p><strong><span id="C"><h1>CHARACTERIZATION OF LALF PROTEIN</h1></span></strong></p><br />
<p>&nbsp;</p><br />
<p><span style="text-decoration: underline;">TARGET</span>: Showing that the LALF protein is produced.</p><br />
<p><strong><em>PROCEDURE 1: </em></strong><strong> </strong></p><br />
<p><strong> </strong></p><br />
<p><strong> </strong><img src="https://static.igem.org/mediawiki/igem.org/1/17/F_1.png"><br />
<strong> </strong></p><br />
<p><strong> </strong><strong> </strong></p><br />
<p><strong> </strong></p><br />
<p><strong> </strong></p><br />
<p><strong> J04500-GFP </strong></p><br />
<p><strong> </strong><strong> </strong></p><br />
<p><strong> <img src="https://static.igem.org/mediawiki/2011/2/20/Jo4450.png"></strong></p><br />
<p><strong> PSB1C3</strong></p><br />
<p><strong> </strong></p><br />
<p>Transformation is done and green colonies are selected then we started to do our second procedure<strong>.</strong></p><br />
<p><img src="https://static.igem.org/mediawiki/2011/9/96/F3.png"><strong><em> </em></strong></p><br />
<p><strong><em> </em></strong></p><br />
<p><strong><em> </em></strong></p><br />
<p><strong><em> </em></strong></p><br />
<p><strong><em> </em></strong></p><br />
<p><strong><em> </em></strong></p><br />
<p><strong><em> </em></strong></p><br />
<p><strong><em> </em></strong></p><br />
<p><strong><em> </em></strong></p><br />
<p><strong><em> </em></strong></p><br />
<p><strong><em> </em></strong></p><br />
<p><strong><em>PROCEDURE 2:</em></strong></p><br />
<p><strong> </strong></p><br />
<p><img src="https://static.igem.org/mediawiki/2011/4/42/F4.png"></p><br />
<p><img src="https://static.igem.org/mediawiki/2011/1/18/F5.png"></p><br />
<p><img src="https://static.igem.org/mediawiki/2011/f/fb/F6.png"></p><br />
<p>Green colonies are selected and picked up then next procedures are completed.</p><br />
<p>1) <em>Plasmid isolation</em></p><br />
<p><em> 2) Digestion</em></p><br />
<p><em>Existence of expected plasmid is confirmed by electrophoresis.</em></p><br />
<p><em> 3) BL21 transformed</em></p><br />
<p><em> 4) BL21 liquid culture planted</em></p><br />
<p><em> 5) RNA isolation</em></p><br />
<p><em> 6) c DNA</em></p><br />
<p><em> 7) PCR</em></p><br />
<p><em>Producing of expected part’s mRNA is confirmed by electrophoresis.</em></p><br />
<p><em> 8) Sequents</em></p><br />
<p><em> </em></p><br />
<p><img src="https://static.igem.org/mediawiki/igem.org/d/d1/Sss1.png"><em> </em></p><br />
<p><span style="text-decoration: underline;">RESULT</span>: Since mRNA of LALF protein has been produced, the promoter is producing both LALF and GFP sequences.</p><br />
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<li><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey/Lab_Garage">Lab Garage</a><br />
<ul><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Notebook">Notebook</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Procedures">Procedures</a></li><br />
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<h2 style="font-family: Verdana, Arial, SunSans-Regular, sans-serif;">Team</h2><br />
<h5 style="font-family: Verdana, Arial, SunSans-Regular, sans-serif;">All Members</h5> <br />
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<tr style="height: 70px; vertical-align: top;"><th colspan="2" style="text-align: center; font-size: 20px; font-weight: bold;">INSTRUCTORS</th></tr><br />
<tr style="height: 200px;" id="15"><br />
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<img src="https://static.igem.org/mediawiki/2011/6/64/Esrahoca.jpg"/><br />
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<big>ESRA GÜNDÜZ</big><br/>(Associate Professor of Medical Genetics)<br />
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<p>Hello everyone! My name is Esra. I’m an Associate Professor of Medical Genetics. This is my third year back in Turkey after staying twelve years in Japan. I mainly work on Cancer Genetics and more specifically on Tumor Suppressor Genes. I do enjoy being with my students and working with them together. iGEM competition has provided us to become closer. I think we share a lot scientifically, socially and friendly together with my students. I also like speaking Japanese and cooking.</p><br />
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<big>KADİR DEMİRCAN</big><br/>(Assistant Professor of Medical Genetics)<br />
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<big>HÜSAMETTİN ERDAMAR</big><br/>(Assistant Professor of Medical Biochemistry)<br />
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<big>CİHAN TAŞTAN</big><br/>(Ph.D Student on Tumor Immunology at Sackler Institue of New York University)<br />
</td></tr><br />
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<td><br />
<p> I am graduate student for Ph.D. on Tumor Immunology at Sackler Institue of New York University. I have participated iGEM competition several times. I have taken parts in the competition as a student member of METU-Gene 2009 (Silver), METU-Turkey Software 2010 (Silver) and METU-Turkey 2010 (Gold). Now, I have been advisor of Fatih University for couple of months in order to start integration of other universities of Turkey into iGEM and to prepare an environment for establisihing and spreading of new synthetic biology labs through Turkey.</p><br />
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<big>MURADİYE ACAR</big><br/>(Ph.D Student on Mecdical Genetics)<br />
</td></tr><br />
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&nbsp; </td><br />
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</td><br />
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<tr style="height: 70px;vertical-align: top;"><th colspan="2" style="text-align: center; font-size: 20px; font-weight: bold;">ATTRIBUTION</th></tr><br />
<br />
<p><tr><td> Canvas Town is a proud product of Fatih Turkey group where all work presented on this wiki is the sole work of the iGEM team members listed above.</td></tr></p><br />
<br />
<br />
<tr style="height: 70px;vertical-align: top;"><th colspan="2" style="text-align: center; font-size: 20px; font-weight: bold;">STUDENTS</th></tr><br />
<br />
<tr style="height: 200px;" id="1"><br />
<td><br />
<img src="https://static.igem.org/mediawiki/2011/0/0a/Alperen.jpg"/><br />
</td><br />
<td width="600" style="padding-left: 20px; padding-bottom: 20px;"><br />
<table style="margin-top:0px;"><br />
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<big>ALPEREN GÜVER</big><br/>Medical Student (2nd Year)<br />
</td></tr><br />
<tr><br />
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<p>I am a first term student of Fatih University Medical Faculty and I will finish first year on July. When I finish my faculty, I want to be a clinician. I like playing tennis and taking some photos.</p><br />
</td><br />
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</table><br />
</td><br />
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<tr style="height: 200px;" id="2"><br />
<td><br />
<img src="https://static.igem.org/mediawiki/2011/f/f7/Betul.jpg"/><br />
</td><br />
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<table style="margin-top:0px;"><br />
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<big>BETÜL GÜNBEY</big><br/>Medical Student (2nd Year)<br />
</td></tr><br />
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<td><br />
<p>I’m a first year student in medical faculty. Also this is my first year in iGEM, I’m interested in clinical medicine; but also, I always like genetics and working in the lab. I think iGEM is a perfect way to learn synthetic biology and teamwork for a student. I like playing guitar. I’m sure our team will be very successful.</p><br />
</td><br />
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</table><br />
</td><br />
</tr><br />
<br />
<tr style="height: 200px;" id="3"><br />
<td><br />
<img src="https://static.igem.org/mediawiki/2011/0/0f/Hasanyasin.jpg"/><br />
</td><br />
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<big>HASAN YASİN SOYLU</big><br/>Medical Student (2nd Year)<br />
</td></tr><br />
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<p>I'm happy to be a part of the team. There is nothing to say about the past time; but I'm here for the future.<br />
"Bismillah is the beginning of every good thing. So, let's say Bismillah!.."</p><br />
</td><br />
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<img src="https://static.igem.org/mediawiki/2011/7/7a/Team-fazilet.jpg"/><br />
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<big>FAZİLET GÜLER</big><br/>Medical Student (3rd Year)<br />
</td></tr><br />
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<p>I finished my second year in medical school on June. I'll be a doctor but I don't want to be a clinician. Thus, genetics and synthetic biology are treasure for me:) This is my first year in iGEM. I'm interested in physics and computer science. I like traveling and hiking. Also, I enjoy riding a roller coaster!</p><br />
</td><br />
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<td><br />
<img src="https://static.igem.org/mediawiki/2011/9/95/Kenangzl.jpg"/><br />
</td><br />
<td width="600" style="padding-left: 20px; padding-bottom: 20px;"><br />
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<big>KENAN OĞUZ GÜZEL</big><br/>Cartoonist<br />
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&nbsp;<br />
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<td><br />
<img src="https://static.igem.org/mediawiki/2011/d/d0/Hale.jpg"/><br />
</td><br />
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<big>HALENUR GÜNGÖRDÜ</big><br/>Medical Student (3rd Year)<br />
</td></tr><br />
<tr><br />
<td><br />
<p>I'm second term student of Fatih University Medical Faculty. This is the first time to attend iGEM competition. In spite of this, we have a great team and our advisors who spend a lot of time for that project. This competition process will be a great advantage for us like working together in solidarity and making idea exchange between us. So this project will broaden our horizon. See you in Holland(o'-'o)//</p><br />
</td><br />
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</td><br />
</tr><br />
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<tr style="height: 200px; "id="7"><br />
<td><br />
<img src="https://static.igem.org/mediawiki/2011/f/ff/Mustafa.jpg"/><br />
</td><br />
<td width="600" style="padding-left: 20px; padding-bottom: 20px;"><br />
<table style="margin-top:0px;"><br />
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<big>MUSTAFA SEMİH ELİTOK</big><br/>Medical Student (2nd Year)<br />
</td></tr><br />
<tr><br />
<td><br />
<p>I'm Mustafa, undergraduate student in Fatih University Medical School. First of all, being a part of this team is an honor. I am so impressed of their patience with me. I am definitely a laid-back person, because my point of view to the life is about not taking the things so seriously. I mean seeing the facts on their funny ways, especially when it gets complicated and seems hard. And the recent studies corrects me that not being stressed is healthy, so relax. And I like playing basketball, chess and poking other people.</p><br />
</td><br />
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<td><br />
<img src="https://static.igem.org/mediawiki/2011/3/30/Secil.jpg"/><br />
</td><br />
<td width="600" style="padding-left: 20px; padding-bottom: 20px;"><br />
<table style="margin-top:0px;"><br />
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<big>SEÇİL TAŞHAN</big><br/>Medical Student (2nd Year)<br />
</td></tr><br />
<tr><br />
<td><br />
<p>This is my first year at Fatih University Medical School as a student. Although my department is the Medicine , I have a great interest in Genetics. After I complete my 6-year medical education , I want to maintain my academic education in Genetics. We are the first medical school in Turkey that participates in iGEM Competition. I hope we will maintain our success for next years, too.. I love watching cartoon :)</p><br />
</td><br />
</tr><br />
</table><br />
</td><br />
</tr><br />
<br />
<tr style="height: 200px;" id="9"><br />
<td><br />
<img src="https://static.igem.org/mediawiki/2011/8/81/Ozantahsin1.jpg"/><br />
</td><br />
<td width="600" style="padding-left: 20px; padding-bottom: 20px;"><br />
<table style="margin-top:0px;"><br />
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<big>OZAN SAMİ BAYRAK</big><br/>Medical Student (2nd Year)<br />
</td></tr><br />
<tr><br />
<td><br />
<p>I'm Ozan! I'm undergraduate student at Fatih University Medical School. I like genetics and synthetic biology, so I'm a part of this team. I advise you to participate in iGEM if you realy love genetics. In addition, I'm a goalkeeper and football is my life. If I didn't choose to be a doctor, I would be a professional goalkeeper.</p><br />
</td><br />
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</td><br />
</tr><br />
<br />
<tr style="height: 200px;" id="10"><br />
<td><br />
<img src="https://static.igem.org/mediawiki/2011/7/72/Tserap1.jpg"/><br />
</td><br />
<td width="600" style="padding-left: 20px; padding-bottom: 20px;"><br />
<table style="margin-top:0px;"><br />
<tr><td><br />
<big>TÜLİN SERAP YILMAZ</big><br/>Medical Student (2nd Year)<br />
</td></tr><br />
<tr><br />
<td><br />
<p>Hi! I m Tülin Serap Yılmaz. I finished my first year in Fatih University Medical School .I m interested in genetics, surgery and psychiatry. I am too bored learning everything in books; so I took part in Team Genesis . We learn everything by seeing. I wish an enjoying summer full with science for you :)</p><br />
</td><br />
</tr><br />
</table><br />
</td><br />
</tr><br />
<br />
<tr style="height: 200px;" id="11"><br />
<td><br />
<img src="https://static.igem.org/mediawiki/2011/a/a3/Yasincakar.jpg"/><br />
</td><br />
<td width="600" style="padding-left: 20px; padding-bottom: 20px;"><br />
<table style="margin-top:0px;"><br />
<tr><td><br />
<big>YASİN ÇAKAR</big><br/>Medical Student (2nd Year)<br />
</td></tr><br />
<tr><br />
<td><br />
<p>Yasin Çakar.. My first year in medicine school.. Glad to being a part of experimental researches in genetics and being a member of Fatih University iGEM Team. Believing that it's loss if your today is equal to yesterday, tomorrow should be different from today..</p><br />
</td><br />
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</table><br />
</td><br />
</tr><br />
<br />
<tr style="height: 200px;" id="12"><br />
<td><br />
<img src="https://static.igem.org/mediawiki/2011/8/83/Buraksimsek.jpg"/><br />
</td><br />
<td width="600" style="padding-left: 20px; padding-bottom: 20px;"><br />
<table style="margin-top:0px;"><br />
<tr><td><br />
<big>BURAK ŞİMŞEK</big><br/>Computer Engineering Student (4th Year)<br />
</td></tr><br />
<tr><br />
<td><br />
&nbsp;<br />
</td><br />
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</table><br />
</td><br />
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Metugen
http://2011.igem.org/Team:Fatih_Turkey/Team
Team:Fatih Turkey/Team
2011-09-22T02:27:40Z
<p>Metugen: </p>
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<a href="https://2011.igem.org/Team:Fatih_Turkey/Project">Project<span>Rainbow Graveyard</span></a><br />
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<h2 style="font-family: Verdana, Arial, SunSans-Regular, sans-serif;">Team</h2><br />
<h5 style="font-family: Verdana, Arial, SunSans-Regular, sans-serif;">All Members</h5> <br />
</div> <br />
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<tr style="height: 70px; vertical-align: top;"><th colspan="2" style="text-align: center; font-size: 20px; font-weight: bold;">INSTRUCTORS</th></tr><br />
<tr style="height: 200px;" id="15"><br />
<td width="166"><br />
<img src="https://static.igem.org/mediawiki/2011/6/64/Esrahoca.jpg"/><br />
</td><br />
<td width="600" style="padding-left: 20px; padding-bottom: 20px;"><br />
<table style="margin-top:0px;"><br />
<tr><td><br />
<big>ESRA GÜNDÜZ</big><br/>(Associate Professor of Medical Genetics)<br />
</td></tr><br />
<tr><br />
<td><br />
<p>Hello everyone! My name is Esra. I’m an Associate Professor of Medical Genetics. This is my third year back in Turkey after staying twelve years in Japan. I mainly work on Cancer Genetics and more specifically on Tumor Suppressor Genes. I do enjoy being with my students and working with them together. iGEM competition has provided us to become closer. I think we share a lot scientifically, socially and friendly together with my students. I also like speaking Japanese and cooking.</p><br />
</td><br />
</tr><br />
</table><br />
</td><br />
</tr><br />
<br />
<tr style="height: 200px;" id="17"><br />
<td><br />
<img src="https://static.igem.org/mediawiki/2011/6/64/Kadirhoca.jpg"/><br />
</td><br />
<td width="600" style="padding-left: 20px; padding-bottom: 20px;"><br />
<table style="margin-top:0px;"><br />
<tr><td><br />
<big>KADİR DEMİRCAN</big><br/>(Assistant Professor of Medical Genetics)<br />
</td></tr><br />
<tr><br />
<td>&nbsp;<br />
</td><br />
</tr><br />
</table><br />
</td><br />
</tr><br />
<br />
<br />
<tr style="height: 200px;" id="16"><br />
<td><br />
<img src="https://static.igem.org/mediawiki/2011/b/b2/Husamettinhoca.jpg"/><br />
</td><br />
<td width="600" style="padding-left: 20px; padding-bottom: 20px;"><br />
<table style="margin-top:0px;"><br />
<tr><td><br />
<big>HÜSAMETTİN ERDAMAR</big><br/>(Assistant Professor of Medical Biochemistry)<br />
</td></tr><br />
<tr><br />
<td>&nbsp;</td><br />
</tr><br />
</table><br />
</td><br />
<br />
</tr><br />
<br />
<tr style="height: 70px;vertical-align: top;"><th colspan="2" style="text-align: center; font-size: 20px; font-weight: bold;">ADVISORS</th></tr><br />
<br />
<tr style="height: 200px;" id="13"><br />
<td><br />
<img src="https://static.igem.org/mediawiki/2011/c/c1/Cihantastan.jpg"/><br />
</td><br />
<td width="600" style="padding-left: 20px; padding-bottom: 20px;"><br />
<table style="margin-top:0px;"><br />
<tr><td><br />
<big>CİHAN TAŞTAN</big><br/>(Ph.D Student on Tumor Immunology at Sackler Institue of New York University)<br />
</td></tr><br />
<tr><br />
<td><br />
<p> I am graduate student for Ph.D. on Tumor Immunology at Sackler Institue of New York University. I have participated iGEM competition several times. I have taken parts in the competition as a student member of METU-Gene 2009 (Silver), METU-Turkey Software 2010 (Silver) and METU-Turkey 2010 (Gold). Now, I have been advisor of Fatih University for couple of months in order to start integration of other universities of Turkey into iGEM and to prepare an environment for establisihing and spreading of new synthetic biology labs through Turkey.</p><br />
</td><br />
</tr><br />
</table><br />
</td><br />
</tr><br />
<br />
<tr style="height: 200px;" id="14"><br />
<td><br />
<img src="https://static.igem.org/mediawiki/2011/e/e3/Muradiye.jpg"/><br />
</td><br />
<td width="600" style="padding-left: 20px; padding-bottom: 20px;"><br />
<table style="margin-top:0px;"><br />
<tr><td><br />
<big>MURADİYE ACAR</big><br/>(Ph.D Student on Mecdical Genetics)<br />
</td></tr><br />
<tr><br />
<td><br />
&nbsp; </td><br />
</tr><br />
</table><br />
</td><br />
</tr><br />
<br />
<tr style="height: 70px;vertical-align: top;"><th colspan="2" style="text-align: center; font-size: 20px; font-weight: bold;">ATTRIBUTION</th></tr><br />
<br />
<div><tr><td> Canvas Town is a proud product of Fatih Turkey group where all work presented on this wiki is the sole work of the iGEM team members listed above.</td></tr></div><br />
<br />
<br />
<tr style="height: 70px;vertical-align: top;"><th colspan="2" style="text-align: center; font-size: 20px; font-weight: bold;">STUDENTS</th></tr><br />
<br />
<tr style="height: 200px;" id="1"><br />
<td><br />
<img src="https://static.igem.org/mediawiki/2011/0/0a/Alperen.jpg"/><br />
</td><br />
<td width="600" style="padding-left: 20px; padding-bottom: 20px;"><br />
<table style="margin-top:0px;"><br />
<tr><td><br />
<big>ALPEREN GÜVER</big><br/>Medical Student (2nd Year)<br />
</td></tr><br />
<tr><br />
<td><br />
<p>I am a first term student of Fatih University Medical Faculty and I will finish first year on July. When I finish my faculty, I want to be a clinician. I like playing tennis and taking some photos.</p><br />
</td><br />
</tr><br />
</table><br />
</td><br />
</tr><br />
<br />
<tr style="height: 200px;" id="2"><br />
<td><br />
<img src="https://static.igem.org/mediawiki/2011/f/f7/Betul.jpg"/><br />
</td><br />
<td width="600" style="padding-left: 20px; padding-bottom: 20px;"><br />
<table style="margin-top:0px;"><br />
<tr><td><br />
<big>BETÜL GÜNBEY</big><br/>Medical Student (2nd Year)<br />
</td></tr><br />
<tr><br />
<td><br />
<p>I’m a first year student in medical faculty. Also this is my first year in iGEM, I’m interested in clinical medicine; but also, I always like genetics and working in the lab. I think iGEM is a perfect way to learn synthetic biology and teamwork for a student. I like playing guitar. I’m sure our team will be very successful.</p><br />
</td><br />
</tr><br />
</table><br />
</td><br />
</tr><br />
<br />
<tr style="height: 200px;" id="3"><br />
<td><br />
<img src="https://static.igem.org/mediawiki/2011/0/0f/Hasanyasin.jpg"/><br />
</td><br />
<td width="600" style="padding-left: 20px; padding-bottom: 20px;"><br />
<table style="margin-top:0px;"><br />
<tr><td><br />
<big>HASAN YASİN SOYLU</big><br/>Medical Student (2nd Year)<br />
</td></tr><br />
<tr><br />
<td><br />
<p>I'm happy to be a part of the team. There is nothing to say about the past time; but I'm here for the future.<br />
"Bismillah is the beginning of every good thing. So, let's say Bismillah!.."</p><br />
</td><br />
</tr><br />
</table><br />
</td><br />
</tr><br />
<br />
<tr style="height: 200px;" id="4"><br />
<td><br />
<img src="https://static.igem.org/mediawiki/2011/7/7a/Team-fazilet.jpg"/><br />
</td><br />
<td width="600" style="padding-left: 20px; padding-bottom: 20px;"><br />
<table style="margin-top:0px;"><br />
<tr><td><br />
<big>FAZİLET GÜLER</big><br/>Medical Student (3rd Year)<br />
</td></tr><br />
<tr><br />
<td><br />
<p>I finished my second year in medical school on June. I'll be a doctor but I don't want to be a clinician. Thus, genetics and synthetic biology are treasure for me:) This is my first year in iGEM. I'm interested in physics and computer science. I like traveling and hiking. Also, I enjoy riding a roller coaster!</p><br />
</td><br />
</tr><br />
</table><br />
</td><br />
</tr><br />
<br />
<tr style="height: 200px;" id="5"><br />
<td><br />
<img src="https://static.igem.org/mediawiki/2011/9/95/Kenangzl.jpg"/><br />
</td><br />
<td width="600" style="padding-left: 20px; padding-bottom: 20px;"><br />
<table style="margin-top:0px;"><br />
<tr><td><br />
<big>KENAN OĞUZ GÜZEL</big><br/>Cartoonist<br />
</td></tr><br />
<tr><br />
<td><br />
&nbsp;<br />
</td><br />
</tr><br />
</table><br />
</td><br />
</tr><br />
<br />
<tr style="height: 200px;" id="6"><br />
<td><br />
<img src="https://static.igem.org/mediawiki/2011/d/d0/Hale.jpg"/><br />
</td><br />
<td width="600" style="padding-left: 20px; padding-bottom: 20px;"><br />
<table style="margin-top:0px;"><br />
<tr><td><br />
<big>HALENUR GÜNGÖRDÜ</big><br/>Medical Student (3rd Year)<br />
</td></tr><br />
<tr><br />
<td><br />
<p>I'm second term student of Fatih University Medical Faculty. This is the first time to attend iGEM competition. In spite of this, we have a great team and our advisors who spend a lot of time for that project. This competition process will be a great advantage for us like working together in solidarity and making idea exchange between us. So this project will broaden our horizon. See you in Holland(o'-'o)//</p><br />
</td><br />
</tr><br />
</table><br />
</td><br />
</tr><br />
<br />
<tr style="height: 200px; "id="7"><br />
<td><br />
<img src="https://static.igem.org/mediawiki/2011/f/ff/Mustafa.jpg"/><br />
</td><br />
<td width="600" style="padding-left: 20px; padding-bottom: 20px;"><br />
<table style="margin-top:0px;"><br />
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<big>MUSTAFA SEMİH ELİTOK</big><br/>Medical Student (2nd Year)<br />
</td></tr><br />
<tr><br />
<td><br />
<p>I'm Mustafa, undergraduate student in Fatih University Medical School. First of all, being a part of this team is an honor. I am so impressed of their patience with me. I am definitely a laid-back person, because my point of view to the life is about not taking the things so seriously. I mean seeing the facts on their funny ways, especially when it gets complicated and seems hard. And the recent studies corrects me that not being stressed is healthy, so relax. And I like playing basketball, chess and poking other people.</p><br />
</td><br />
</tr><br />
</table><br />
</td><br />
</tr><br />
<br />
<tr style="height: 200px;" id="8"><br />
<td><br />
<img src="https://static.igem.org/mediawiki/2011/3/30/Secil.jpg"/><br />
</td><br />
<td width="600" style="padding-left: 20px; padding-bottom: 20px;"><br />
<table style="margin-top:0px;"><br />
<tr><td><br />
<big>SEÇİL TAŞHAN</big><br/>Medical Student (2nd Year)<br />
</td></tr><br />
<tr><br />
<td><br />
<p>This is my first year at Fatih University Medical School as a student. Although my department is the Medicine , I have a great interest in Genetics. After I complete my 6-year medical education , I want to maintain my academic education in Genetics. We are the first medical school in Turkey that participates in iGEM Competition. I hope we will maintain our success for next years, too.. I love watching cartoon :)</p><br />
</td><br />
</tr><br />
</table><br />
</td><br />
</tr><br />
<br />
<tr style="height: 200px;" id="9"><br />
<td><br />
<img src="https://static.igem.org/mediawiki/2011/8/81/Ozantahsin1.jpg"/><br />
</td><br />
<td width="600" style="padding-left: 20px; padding-bottom: 20px;"><br />
<table style="margin-top:0px;"><br />
<tr><td><br />
<big>OZAN SAMİ BAYRAK</big><br/>Medical Student (2nd Year)<br />
</td></tr><br />
<tr><br />
<td><br />
<p>I'm Ozan! I'm undergraduate student at Fatih University Medical School. I like genetics and synthetic biology, so I'm a part of this team. I advise you to participate in iGEM if you realy love genetics. In addition, I'm a goalkeeper and football is my life. If I didn't choose to be a doctor, I would be a professional goalkeeper.</p><br />
</td><br />
</tr><br />
</table><br />
</td><br />
</tr><br />
<br />
<tr style="height: 200px;" id="10"><br />
<td><br />
<img src="https://static.igem.org/mediawiki/2011/7/72/Tserap1.jpg"/><br />
</td><br />
<td width="600" style="padding-left: 20px; padding-bottom: 20px;"><br />
<table style="margin-top:0px;"><br />
<tr><td><br />
<big>TÜLİN SERAP YILMAZ</big><br/>Medical Student (2nd Year)<br />
</td></tr><br />
<tr><br />
<td><br />
<p>Hi! I m Tülin Serap Yılmaz. I finished my first year in Fatih University Medical School .I m interested in genetics, surgery and psychiatry. I am too bored learning everything in books; so I took part in Team Genesis . We learn everything by seeing. I wish an enjoying summer full with science for you :)</p><br />
</td><br />
</tr><br />
</table><br />
</td><br />
</tr><br />
<br />
<tr style="height: 200px;" id="11"><br />
<td><br />
<img src="https://static.igem.org/mediawiki/2011/a/a3/Yasincakar.jpg"/><br />
</td><br />
<td width="600" style="padding-left: 20px; padding-bottom: 20px;"><br />
<table style="margin-top:0px;"><br />
<tr><td><br />
<big>YASİN ÇAKAR</big><br/>Medical Student (2nd Year)<br />
</td></tr><br />
<tr><br />
<td><br />
<p>Yasin Çakar.. My first year in medicine school.. Glad to being a part of experimental researches in genetics and being a member of Fatih University iGEM Team. Believing that it's loss if your today is equal to yesterday, tomorrow should be different from today..</p><br />
</td><br />
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</td><br />
</tr><br />
<br />
<tr style="height: 200px;" id="12"><br />
<td><br />
<img src="https://static.igem.org/mediawiki/2011/8/83/Buraksimsek.jpg"/><br />
</td><br />
<td width="600" style="padding-left: 20px; padding-bottom: 20px;"><br />
<table style="margin-top:0px;"><br />
<tr><td><br />
<big>BURAK ŞİMŞEK</big><br/>Computer Engineering Student (4th Year)<br />
</td></tr><br />
<tr><br />
<td><br />
&nbsp;<br />
</td><br />
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</table><br />
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Metugen
http://2011.igem.org/Team:Fatih_Turkey/Team
Team:Fatih Turkey/Team
2011-09-22T02:26:34Z
<p>Metugen: </p>
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<h2 style="font-family: Verdana, Arial, SunSans-Regular, sans-serif;">Team</h2><br />
<h5 style="font-family: Verdana, Arial, SunSans-Regular, sans-serif;">All Members</h5> <br />
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<tr style="height: 70px; vertical-align: top;"><th colspan="2" style="text-align: center; font-size: 20px; font-weight: bold;">INSTRUCTORS</th></tr><br />
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<big>ESRA GÜNDÜZ</big><br/>(Associate Professor of Medical Genetics)<br />
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<p>Hello everyone! My name is Esra. I’m an Associate Professor of Medical Genetics. This is my third year back in Turkey after staying twelve years in Japan. I mainly work on Cancer Genetics and more specifically on Tumor Suppressor Genes. I do enjoy being with my students and working with them together. iGEM competition has provided us to become closer. I think we share a lot scientifically, socially and friendly together with my students. I also like speaking Japanese and cooking.</p><br />
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<big>KADİR DEMİRCAN</big><br/>(Assistant Professor of Medical Genetics)<br />
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<big>HÜSAMETTİN ERDAMAR</big><br/>(Assistant Professor of Medical Biochemistry)<br />
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<big>CİHAN TAŞTAN</big><br/>(Ph.D Student on Tumor Immunology at Sackler Institue of New York University)<br />
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<p> I am graduate student for Ph.D. on Tumor Immunology at Sackler Institue of New York University. I have participated iGEM competition several times. I have taken parts in the competition as a student member of METU-Gene 2009 (Silver), METU-Turkey Software 2010 (Silver) and METU-Turkey 2010 (Gold). Now, I have been advisor of Fatih University for couple of months in order to start integration of other universities of Turkey into iGEM and to prepare an environment for establisihing and spreading of new synthetic biology labs through Turkey.</p><br />
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<big>MURADİYE ACAR</big><br/>(Ph.D Student on Mecdical Genetics)<br />
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<tr style="height: 70px;vertical-align: top;"><th colspan="2" style="text-align: center; font-size: 20px; font-weight: bold;">ATTRIBUTION</th></tr><br />
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<div> Canvas Town is a proud product of Fatih Turkey group where all work presented on this wiki is the sole work of the iGEM team members listed above.</div><br />
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<big>ALPEREN GÜVER</big><br/>Medical Student (2nd Year)<br />
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<p>I am a first term student of Fatih University Medical Faculty and I will finish first year on July. When I finish my faculty, I want to be a clinician. I like playing tennis and taking some photos.</p><br />
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<big>BETÜL GÜNBEY</big><br/>Medical Student (2nd Year)<br />
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<p>I’m a first year student in medical faculty. Also this is my first year in iGEM, I’m interested in clinical medicine; but also, I always like genetics and working in the lab. I think iGEM is a perfect way to learn synthetic biology and teamwork for a student. I like playing guitar. I’m sure our team will be very successful.</p><br />
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<big>HASAN YASİN SOYLU</big><br/>Medical Student (2nd Year)<br />
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<p>I'm happy to be a part of the team. There is nothing to say about the past time; but I'm here for the future.<br />
"Bismillah is the beginning of every good thing. So, let's say Bismillah!.."</p><br />
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<big>FAZİLET GÜLER</big><br/>Medical Student (3rd Year)<br />
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<p>I finished my second year in medical school on June. I'll be a doctor but I don't want to be a clinician. Thus, genetics and synthetic biology are treasure for me:) This is my first year in iGEM. I'm interested in physics and computer science. I like traveling and hiking. Also, I enjoy riding a roller coaster!</p><br />
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<big>KENAN OĞUZ GÜZEL</big><br/>Cartoonist<br />
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<big>HALENUR GÜNGÖRDÜ</big><br/>Medical Student (3rd Year)<br />
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<p>I'm second term student of Fatih University Medical Faculty. This is the first time to attend iGEM competition. In spite of this, we have a great team and our advisors who spend a lot of time for that project. This competition process will be a great advantage for us like working together in solidarity and making idea exchange between us. So this project will broaden our horizon. See you in Holland(o'-'o)//</p><br />
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<big>MUSTAFA SEMİH ELİTOK</big><br/>Medical Student (2nd Year)<br />
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<p>I'm Mustafa, undergraduate student in Fatih University Medical School. First of all, being a part of this team is an honor. I am so impressed of their patience with me. I am definitely a laid-back person, because my point of view to the life is about not taking the things so seriously. I mean seeing the facts on their funny ways, especially when it gets complicated and seems hard. And the recent studies corrects me that not being stressed is healthy, so relax. And I like playing basketball, chess and poking other people.</p><br />
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<big>SEÇİL TAŞHAN</big><br/>Medical Student (2nd Year)<br />
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<p>This is my first year at Fatih University Medical School as a student. Although my department is the Medicine , I have a great interest in Genetics. After I complete my 6-year medical education , I want to maintain my academic education in Genetics. We are the first medical school in Turkey that participates in iGEM Competition. I hope we will maintain our success for next years, too.. I love watching cartoon :)</p><br />
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<big>OZAN SAMİ BAYRAK</big><br/>Medical Student (2nd Year)<br />
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<p>I'm Ozan! I'm undergraduate student at Fatih University Medical School. I like genetics and synthetic biology, so I'm a part of this team. I advise you to participate in iGEM if you realy love genetics. In addition, I'm a goalkeeper and football is my life. If I didn't choose to be a doctor, I would be a professional goalkeeper.</p><br />
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<big>TÜLİN SERAP YILMAZ</big><br/>Medical Student (2nd Year)<br />
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<p>Hi! I m Tülin Serap Yılmaz. I finished my first year in Fatih University Medical School .I m interested in genetics, surgery and psychiatry. I am too bored learning everything in books; so I took part in Team Genesis . We learn everything by seeing. I wish an enjoying summer full with science for you :)</p><br />
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<big>YASİN ÇAKAR</big><br/>Medical Student (2nd Year)<br />
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<p>Yasin Çakar.. My first year in medicine school.. Glad to being a part of experimental researches in genetics and being a member of Fatih University iGEM Team. Believing that it's loss if your today is equal to yesterday, tomorrow should be different from today..</p><br />
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<big>BURAK ŞİMŞEK</big><br/>Computer Engineering Student (4th Year)<br />
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Metugen
http://2011.igem.org/Team:Fatih_Turkey/Team
Team:Fatih Turkey/Team
2011-09-22T02:25:48Z
<p>Metugen: </p>
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<a href="https://2011.igem.org/Team:Fatih_Turkey/Project">Project<span>Rainbow Graveyard</span></a><br />
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<h2 style="font-family: Verdana, Arial, SunSans-Regular, sans-serif;">Team</h2><br />
<h5 style="font-family: Verdana, Arial, SunSans-Regular, sans-serif;">All Members</h5> <br />
</div> <br />
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<tr style="height: 70px; vertical-align: top;"><th colspan="2" style="text-align: center; font-size: 20px; font-weight: bold;">INSTRUCTORS</th></tr><br />
<tr style="height: 200px;" id="15"><br />
<td width="166"><br />
<img src="https://static.igem.org/mediawiki/2011/6/64/Esrahoca.jpg"/><br />
</td><br />
<td width="600" style="padding-left: 20px; padding-bottom: 20px;"><br />
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<tr><td><br />
<big>ESRA GÜNDÜZ</big><br/>(Associate Professor of Medical Genetics)<br />
</td></tr><br />
<tr><br />
<td><br />
<p>Hello everyone! My name is Esra. I’m an Associate Professor of Medical Genetics. This is my third year back in Turkey after staying twelve years in Japan. I mainly work on Cancer Genetics and more specifically on Tumor Suppressor Genes. I do enjoy being with my students and working with them together. iGEM competition has provided us to become closer. I think we share a lot scientifically, socially and friendly together with my students. I also like speaking Japanese and cooking.</p><br />
</td><br />
</tr><br />
</table><br />
</td><br />
</tr><br />
<br />
<tr style="height: 200px;" id="17"><br />
<td><br />
<img src="https://static.igem.org/mediawiki/2011/6/64/Kadirhoca.jpg"/><br />
</td><br />
<td width="600" style="padding-left: 20px; padding-bottom: 20px;"><br />
<table style="margin-top:0px;"><br />
<tr><td><br />
<big>KADİR DEMİRCAN</big><br/>(Assistant Professor of Medical Genetics)<br />
</td></tr><br />
<tr><br />
<td>&nbsp;<br />
</td><br />
</tr><br />
</table><br />
</td><br />
</tr><br />
<br />
<br />
<tr style="height: 200px;" id="16"><br />
<td><br />
<img src="https://static.igem.org/mediawiki/2011/b/b2/Husamettinhoca.jpg"/><br />
</td><br />
<td width="600" style="padding-left: 20px; padding-bottom: 20px;"><br />
<table style="margin-top:0px;"><br />
<tr><td><br />
<big>HÜSAMETTİN ERDAMAR</big><br/>(Assistant Professor of Medical Biochemistry)<br />
</td></tr><br />
<tr><br />
<td>&nbsp;</td><br />
</tr><br />
</table><br />
</td><br />
<br />
</tr><br />
<br />
<tr style="height: 70px;vertical-align: top;"><th colspan="2" style="text-align: center; font-size: 20px; font-weight: bold;">ADVISORS</th></tr><br />
<br />
<tr style="height: 200px;" id="13"><br />
<td><br />
<img src="https://static.igem.org/mediawiki/2011/c/c1/Cihantastan.jpg"/><br />
</td><br />
<td width="600" style="padding-left: 20px; padding-bottom: 20px;"><br />
<table style="margin-top:0px;"><br />
<tr><td><br />
<big>CİHAN TAŞTAN</big><br/>(Ph.D Student on Tumor Immunology at Sackler Institue of New York University)<br />
</td></tr><br />
<tr><br />
<td><br />
<p> I am graduate student for Ph.D. on Tumor Immunology at Sackler Institue of New York University. I have participated iGEM competition several times. I have taken parts in the competition as a student member of METU-Gene 2009 (Silver), METU-Turkey Software 2010 (Silver) and METU-Turkey 2010 (Gold). Now, I have been advisor of Fatih University for couple of months in order to start integration of other universities of Turkey into iGEM and to prepare an environment for establisihing and spreading of new synthetic biology labs through Turkey.</p><br />
</td><br />
</tr><br />
</table><br />
</td><br />
</tr><br />
<br />
<tr style="height: 200px;" id="14"><br />
<td><br />
<img src="https://static.igem.org/mediawiki/2011/e/e3/Muradiye.jpg"/><br />
</td><br />
<td width="600" style="padding-left: 20px; padding-bottom: 20px;"><br />
<table style="margin-top:0px;"><br />
<tr><td><br />
<big>MURADİYE ACAR</big><br/>(Ph.D Student on Mecdical Genetics)<br />
</td></tr><br />
<tr><br />
<td><br />
&nbsp; </td><br />
</tr><br />
</table><br />
</td><br />
</tr><br />
<br />
<tr style="height: 70px;vertical-align: top;"><th colspan="2" style="text-align: center; font-size: 20px; font-weight: bold;">ATTRIBUTION</th></tr><br />
<br />
Canvas Town is a proud product of Fatih Turkey group where all work presented on this wiki is the sole work of the iGEM team members listed above.<br />
<br />
<br />
<br />
<tr style="height: 70px;vertical-align: top;"><th colspan="2" style="text-align: center; font-size: 20px; font-weight: bold;">STUDENTS</th></tr><br />
<br />
<tr style="height: 200px;" id="1"><br />
<td><br />
<img src="https://static.igem.org/mediawiki/2011/0/0a/Alperen.jpg"/><br />
</td><br />
<td width="600" style="padding-left: 20px; padding-bottom: 20px;"><br />
<table style="margin-top:0px;"><br />
<tr><td><br />
<big>ALPEREN GÜVER</big><br/>Medical Student (2nd Year)<br />
</td></tr><br />
<tr><br />
<td><br />
<p>I am a first term student of Fatih University Medical Faculty and I will finish first year on July. When I finish my faculty, I want to be a clinician. I like playing tennis and taking some photos.</p><br />
</td><br />
</tr><br />
</table><br />
</td><br />
</tr><br />
<br />
<tr style="height: 200px;" id="2"><br />
<td><br />
<img src="https://static.igem.org/mediawiki/2011/f/f7/Betul.jpg"/><br />
</td><br />
<td width="600" style="padding-left: 20px; padding-bottom: 20px;"><br />
<table style="margin-top:0px;"><br />
<tr><td><br />
<big>BETÜL GÜNBEY</big><br/>Medical Student (2nd Year)<br />
</td></tr><br />
<tr><br />
<td><br />
<p>I’m a first year student in medical faculty. Also this is my first year in iGEM, I’m interested in clinical medicine; but also, I always like genetics and working in the lab. I think iGEM is a perfect way to learn synthetic biology and teamwork for a student. I like playing guitar. I’m sure our team will be very successful.</p><br />
</td><br />
</tr><br />
</table><br />
</td><br />
</tr><br />
<br />
<tr style="height: 200px;" id="3"><br />
<td><br />
<img src="https://static.igem.org/mediawiki/2011/0/0f/Hasanyasin.jpg"/><br />
</td><br />
<td width="600" style="padding-left: 20px; padding-bottom: 20px;"><br />
<table style="margin-top:0px;"><br />
<tr><td><br />
<big>HASAN YASİN SOYLU</big><br/>Medical Student (2nd Year)<br />
</td></tr><br />
<tr><br />
<td><br />
<p>I'm happy to be a part of the team. There is nothing to say about the past time; but I'm here for the future.<br />
"Bismillah is the beginning of every good thing. So, let's say Bismillah!.."</p><br />
</td><br />
</tr><br />
</table><br />
</td><br />
</tr><br />
<br />
<tr style="height: 200px;" id="4"><br />
<td><br />
<img src="https://static.igem.org/mediawiki/2011/7/7a/Team-fazilet.jpg"/><br />
</td><br />
<td width="600" style="padding-left: 20px; padding-bottom: 20px;"><br />
<table style="margin-top:0px;"><br />
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<big>FAZİLET GÜLER</big><br/>Medical Student (3rd Year)<br />
</td></tr><br />
<tr><br />
<td><br />
<p>I finished my second year in medical school on June. I'll be a doctor but I don't want to be a clinician. Thus, genetics and synthetic biology are treasure for me:) This is my first year in iGEM. I'm interested in physics and computer science. I like traveling and hiking. Also, I enjoy riding a roller coaster!</p><br />
</td><br />
</tr><br />
</table><br />
</td><br />
</tr><br />
<br />
<tr style="height: 200px;" id="5"><br />
<td><br />
<img src="https://static.igem.org/mediawiki/2011/9/95/Kenangzl.jpg"/><br />
</td><br />
<td width="600" style="padding-left: 20px; padding-bottom: 20px;"><br />
<table style="margin-top:0px;"><br />
<tr><td><br />
<big>KENAN OĞUZ GÜZEL</big><br/>Cartoonist<br />
</td></tr><br />
<tr><br />
<td><br />
&nbsp;<br />
</td><br />
</tr><br />
</table><br />
</td><br />
</tr><br />
<br />
<tr style="height: 200px;" id="6"><br />
<td><br />
<img src="https://static.igem.org/mediawiki/2011/d/d0/Hale.jpg"/><br />
</td><br />
<td width="600" style="padding-left: 20px; padding-bottom: 20px;"><br />
<table style="margin-top:0px;"><br />
<tr><td><br />
<big>HALENUR GÜNGÖRDÜ</big><br/>Medical Student (3rd Year)<br />
</td></tr><br />
<tr><br />
<td><br />
<p>I'm second term student of Fatih University Medical Faculty. This is the first time to attend iGEM competition. In spite of this, we have a great team and our advisors who spend a lot of time for that project. This competition process will be a great advantage for us like working together in solidarity and making idea exchange between us. So this project will broaden our horizon. See you in Holland(o'-'o)//</p><br />
</td><br />
</tr><br />
</table><br />
</td><br />
</tr><br />
<br />
<tr style="height: 200px; "id="7"><br />
<td><br />
<img src="https://static.igem.org/mediawiki/2011/f/ff/Mustafa.jpg"/><br />
</td><br />
<td width="600" style="padding-left: 20px; padding-bottom: 20px;"><br />
<table style="margin-top:0px;"><br />
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<big>MUSTAFA SEMİH ELİTOK</big><br/>Medical Student (2nd Year)<br />
</td></tr><br />
<tr><br />
<td><br />
<p>I'm Mustafa, undergraduate student in Fatih University Medical School. First of all, being a part of this team is an honor. I am so impressed of their patience with me. I am definitely a laid-back person, because my point of view to the life is about not taking the things so seriously. I mean seeing the facts on their funny ways, especially when it gets complicated and seems hard. And the recent studies corrects me that not being stressed is healthy, so relax. And I like playing basketball, chess and poking other people.</p><br />
</td><br />
</tr><br />
</table><br />
</td><br />
</tr><br />
<br />
<tr style="height: 200px;" id="8"><br />
<td><br />
<img src="https://static.igem.org/mediawiki/2011/3/30/Secil.jpg"/><br />
</td><br />
<td width="600" style="padding-left: 20px; padding-bottom: 20px;"><br />
<table style="margin-top:0px;"><br />
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<big>SEÇİL TAŞHAN</big><br/>Medical Student (2nd Year)<br />
</td></tr><br />
<tr><br />
<td><br />
<p>This is my first year at Fatih University Medical School as a student. Although my department is the Medicine , I have a great interest in Genetics. After I complete my 6-year medical education , I want to maintain my academic education in Genetics. We are the first medical school in Turkey that participates in iGEM Competition. I hope we will maintain our success for next years, too.. I love watching cartoon :)</p><br />
</td><br />
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</table><br />
</td><br />
</tr><br />
<br />
<tr style="height: 200px;" id="9"><br />
<td><br />
<img src="https://static.igem.org/mediawiki/2011/8/81/Ozantahsin1.jpg"/><br />
</td><br />
<td width="600" style="padding-left: 20px; padding-bottom: 20px;"><br />
<table style="margin-top:0px;"><br />
<tr><td><br />
<big>OZAN SAMİ BAYRAK</big><br/>Medical Student (2nd Year)<br />
</td></tr><br />
<tr><br />
<td><br />
<p>I'm Ozan! I'm undergraduate student at Fatih University Medical School. I like genetics and synthetic biology, so I'm a part of this team. I advise you to participate in iGEM if you realy love genetics. In addition, I'm a goalkeeper and football is my life. If I didn't choose to be a doctor, I would be a professional goalkeeper.</p><br />
</td><br />
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<br />
<tr style="height: 200px;" id="10"><br />
<td><br />
<img src="https://static.igem.org/mediawiki/2011/7/72/Tserap1.jpg"/><br />
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<td width="600" style="padding-left: 20px; padding-bottom: 20px;"><br />
<table style="margin-top:0px;"><br />
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<big>TÜLİN SERAP YILMAZ</big><br/>Medical Student (2nd Year)<br />
</td></tr><br />
<tr><br />
<td><br />
<p>Hi! I m Tülin Serap Yılmaz. I finished my first year in Fatih University Medical School .I m interested in genetics, surgery and psychiatry. I am too bored learning everything in books; so I took part in Team Genesis . We learn everything by seeing. I wish an enjoying summer full with science for you :)</p><br />
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<br />
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<td><br />
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<big>YASİN ÇAKAR</big><br/>Medical Student (2nd Year)<br />
</td></tr><br />
<tr><br />
<td><br />
<p>Yasin Çakar.. My first year in medicine school.. Glad to being a part of experimental researches in genetics and being a member of Fatih University iGEM Team. Believing that it's loss if your today is equal to yesterday, tomorrow should be different from today..</p><br />
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<big>BURAK ŞİMŞEK</big><br/>Computer Engineering Student (4th Year)<br />
</td></tr><br />
<tr><br />
<td><br />
&nbsp;<br />
</td><br />
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Metugen
http://2011.igem.org/Team:Fatih_Turkey/Team
Team:Fatih Turkey/Team
2011-09-22T02:25:00Z
<p>Metugen: </p>
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<img src="https://static.igem.org/mediawiki/2011/6/64/Esrahoca.jpg"/><br />
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<big>ESRA GÜNDÜZ</big><br/>(Associate Professor of Medical Genetics)<br />
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<p>Hello everyone! My name is Esra. I’m an Associate Professor of Medical Genetics. This is my third year back in Turkey after staying twelve years in Japan. I mainly work on Cancer Genetics and more specifically on Tumor Suppressor Genes. I do enjoy being with my students and working with them together. iGEM competition has provided us to become closer. I think we share a lot scientifically, socially and friendly together with my students. I also like speaking Japanese and cooking.</p><br />
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<big>KADİR DEMİRCAN</big><br/>(Assistant Professor of Medical Genetics)<br />
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<big>HÜSAMETTİN ERDAMAR</big><br/>(Assistant Professor of Medical Biochemistry)<br />
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<img src="https://static.igem.org/mediawiki/2011/c/c1/Cihantastan.jpg"/><br />
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<big>CİHAN TAŞTAN</big><br/>(Ph.D Student on Tumor Immunology at Sackler Institue of New York University)<br />
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<p> I am graduate student for Ph.D. on Tumor Immunology at Sackler Institue of New York University. I have participated iGEM competition several times. I have taken parts in the competition as a student member of METU-Gene 2009 (Silver), METU-Turkey Software 2010 (Silver) and METU-Turkey 2010 (Gold). Now, I have been advisor of Fatih University for couple of months in order to start integration of other universities of Turkey into iGEM and to prepare an environment for establisihing and spreading of new synthetic biology labs through Turkey.</p><br />
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<big>MURADİYE ACAR</big><br/>(Ph.D Student on Mecdical Genetics)<br />
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&nbsp; </td><br />
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<tr style="height: 70px;vertical-align: top;">ATTRIBUTION</tr><br />
<td><br />
Canvas Town is a proud product of Fatih Turkey group where all work presented on this wiki is the sole work of the iGEM team members listed above.<br />
</td><br />
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<tr style="height: 70px;vertical-align: top;"><th colspan="2" style="text-align: center; font-size: 20px; font-weight: bold;">STUDENTS</th></tr><br />
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<img src="https://static.igem.org/mediawiki/2011/0/0a/Alperen.jpg"/><br />
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<big>ALPEREN GÜVER</big><br/>Medical Student (2nd Year)<br />
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<p>I am a first term student of Fatih University Medical Faculty and I will finish first year on July. When I finish my faculty, I want to be a clinician. I like playing tennis and taking some photos.</p><br />
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<img src="https://static.igem.org/mediawiki/2011/f/f7/Betul.jpg"/><br />
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<big>BETÜL GÜNBEY</big><br/>Medical Student (2nd Year)<br />
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<p>I’m a first year student in medical faculty. Also this is my first year in iGEM, I’m interested in clinical medicine; but also, I always like genetics and working in the lab. I think iGEM is a perfect way to learn synthetic biology and teamwork for a student. I like playing guitar. I’m sure our team will be very successful.</p><br />
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<img src="https://static.igem.org/mediawiki/2011/0/0f/Hasanyasin.jpg"/><br />
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<big>HASAN YASİN SOYLU</big><br/>Medical Student (2nd Year)<br />
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<p>I'm happy to be a part of the team. There is nothing to say about the past time; but I'm here for the future.<br />
"Bismillah is the beginning of every good thing. So, let's say Bismillah!.."</p><br />
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<img src="https://static.igem.org/mediawiki/2011/7/7a/Team-fazilet.jpg"/><br />
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<big>FAZİLET GÜLER</big><br/>Medical Student (3rd Year)<br />
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<p>I finished my second year in medical school on June. I'll be a doctor but I don't want to be a clinician. Thus, genetics and synthetic biology are treasure for me:) This is my first year in iGEM. I'm interested in physics and computer science. I like traveling and hiking. Also, I enjoy riding a roller coaster!</p><br />
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<img src="https://static.igem.org/mediawiki/2011/9/95/Kenangzl.jpg"/><br />
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<big>KENAN OĞUZ GÜZEL</big><br/>Cartoonist<br />
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&nbsp;<br />
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<big>HALENUR GÜNGÖRDÜ</big><br/>Medical Student (3rd Year)<br />
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<td><br />
<p>I'm second term student of Fatih University Medical Faculty. This is the first time to attend iGEM competition. In spite of this, we have a great team and our advisors who spend a lot of time for that project. This competition process will be a great advantage for us like working together in solidarity and making idea exchange between us. So this project will broaden our horizon. See you in Holland(o'-'o)//</p><br />
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<img src="https://static.igem.org/mediawiki/2011/f/ff/Mustafa.jpg"/><br />
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<big>MUSTAFA SEMİH ELİTOK</big><br/>Medical Student (2nd Year)<br />
</td></tr><br />
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<td><br />
<p>I'm Mustafa, undergraduate student in Fatih University Medical School. First of all, being a part of this team is an honor. I am so impressed of their patience with me. I am definitely a laid-back person, because my point of view to the life is about not taking the things so seriously. I mean seeing the facts on their funny ways, especially when it gets complicated and seems hard. And the recent studies corrects me that not being stressed is healthy, so relax. And I like playing basketball, chess and poking other people.</p><br />
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<big>SEÇİL TAŞHAN</big><br/>Medical Student (2nd Year)<br />
</td></tr><br />
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<td><br />
<p>This is my first year at Fatih University Medical School as a student. Although my department is the Medicine , I have a great interest in Genetics. After I complete my 6-year medical education , I want to maintain my academic education in Genetics. We are the first medical school in Turkey that participates in iGEM Competition. I hope we will maintain our success for next years, too.. I love watching cartoon :)</p><br />
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<img src="https://static.igem.org/mediawiki/2011/8/81/Ozantahsin1.jpg"/><br />
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<big>OZAN SAMİ BAYRAK</big><br/>Medical Student (2nd Year)<br />
</td></tr><br />
<tr><br />
<td><br />
<p>I'm Ozan! I'm undergraduate student at Fatih University Medical School. I like genetics and synthetic biology, so I'm a part of this team. I advise you to participate in iGEM if you realy love genetics. In addition, I'm a goalkeeper and football is my life. If I didn't choose to be a doctor, I would be a professional goalkeeper.</p><br />
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<td><br />
<img src="https://static.igem.org/mediawiki/2011/7/72/Tserap1.jpg"/><br />
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<big>TÜLİN SERAP YILMAZ</big><br/>Medical Student (2nd Year)<br />
</td></tr><br />
<tr><br />
<td><br />
<p>Hi! I m Tülin Serap Yılmaz. I finished my first year in Fatih University Medical School .I m interested in genetics, surgery and psychiatry. I am too bored learning everything in books; so I took part in Team Genesis . We learn everything by seeing. I wish an enjoying summer full with science for you :)</p><br />
</td><br />
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<tr style="height: 200px;" id="11"><br />
<td><br />
<img src="https://static.igem.org/mediawiki/2011/a/a3/Yasincakar.jpg"/><br />
</td><br />
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<big>YASİN ÇAKAR</big><br/>Medical Student (2nd Year)<br />
</td></tr><br />
<tr><br />
<td><br />
<p>Yasin Çakar.. My first year in medicine school.. Glad to being a part of experimental researches in genetics and being a member of Fatih University iGEM Team. Believing that it's loss if your today is equal to yesterday, tomorrow should be different from today..</p><br />
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<td><br />
<img src="https://static.igem.org/mediawiki/2011/8/83/Buraksimsek.jpg"/><br />
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<td width="600" style="padding-left: 20px; padding-bottom: 20px;"><br />
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<big>BURAK ŞİMŞEK</big><br/>Computer Engineering Student (4th Year)<br />
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&nbsp;<br />
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<h3 style="color:#BBB;">Sponsors</h3><br />
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Metugen
http://2011.igem.org/Team:Fatih_Turkey/Team
Team:Fatih Turkey/Team
2011-09-22T02:23:15Z
<p>Metugen: </p>
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<a href="https://2011.igem.org/Team:Fatih_Turkey/Project">Project<span>Rainbow Graveyard</span></a><br />
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<h2 style="font-family: Verdana, Arial, SunSans-Regular, sans-serif;">Team</h2><br />
<h5 style="font-family: Verdana, Arial, SunSans-Regular, sans-serif;">All Members</h5> <br />
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<tr style="height: 70px; vertical-align: top;"><th colspan="2" style="text-align: center; font-size: 20px; font-weight: bold;">INSTRUCTORS</th></tr><br />
<tr style="height: 200px;" id="15"><br />
<td width="166"><br />
<img src="https://static.igem.org/mediawiki/2011/6/64/Esrahoca.jpg"/><br />
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<td width="600" style="padding-left: 20px; padding-bottom: 20px;"><br />
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<big>ESRA GÜNDÜZ</big><br/>(Associate Professor of Medical Genetics)<br />
</td></tr><br />
<tr><br />
<td><br />
<p>Hello everyone! My name is Esra. I’m an Associate Professor of Medical Genetics. This is my third year back in Turkey after staying twelve years in Japan. I mainly work on Cancer Genetics and more specifically on Tumor Suppressor Genes. I do enjoy being with my students and working with them together. iGEM competition has provided us to become closer. I think we share a lot scientifically, socially and friendly together with my students. I also like speaking Japanese and cooking.</p><br />
</td><br />
</tr><br />
</table><br />
</td><br />
</tr><br />
<br />
<tr style="height: 200px;" id="17"><br />
<td><br />
<img src="https://static.igem.org/mediawiki/2011/6/64/Kadirhoca.jpg"/><br />
</td><br />
<td width="600" style="padding-left: 20px; padding-bottom: 20px;"><br />
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<big>KADİR DEMİRCAN</big><br/>(Assistant Professor of Medical Genetics)<br />
</td></tr><br />
<tr><br />
<td>&nbsp;<br />
</td><br />
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</td><br />
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<br />
<br />
<tr style="height: 200px;" id="16"><br />
<td><br />
<img src="https://static.igem.org/mediawiki/2011/b/b2/Husamettinhoca.jpg"/><br />
</td><br />
<td width="600" style="padding-left: 20px; padding-bottom: 20px;"><br />
<table style="margin-top:0px;"><br />
<tr><td><br />
<big>HÜSAMETTİN ERDAMAR</big><br/>(Assistant Professor of Medical Biochemistry)<br />
</td></tr><br />
<tr><br />
<td>&nbsp;</td><br />
</tr><br />
</table><br />
</td><br />
<br />
</tr><br />
<br />
<tr style="height: 70px;vertical-align: top;"><th colspan="2" style="text-align: center; font-size: 20px; font-weight: bold;">ADVISORS</th></tr><br />
<br />
<tr style="height: 200px;" id="13"><br />
<td><br />
<img src="https://static.igem.org/mediawiki/2011/c/c1/Cihantastan.jpg"/><br />
</td><br />
<td width="600" style="padding-left: 20px; padding-bottom: 20px;"><br />
<table style="margin-top:0px;"><br />
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<big>CİHAN TAŞTAN</big><br/>(Ph.D Student on Tumor Immunology at Sackler Institue of New York University)<br />
</td></tr><br />
<tr><br />
<td><br />
<p> I am graduate student for Ph.D. on Tumor Immunology at Sackler Institue of New York University. I have participated iGEM competition several times. I have taken parts in the competition as a student member of METU-Gene 2009 (Silver), METU-Turkey Software 2010 (Silver) and METU-Turkey 2010 (Gold). Now, I have been advisor of Fatih University for couple of months in order to start integration of other universities of Turkey into iGEM and to prepare an environment for establisihing and spreading of new synthetic biology labs through Turkey.</p><br />
</td><br />
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</table><br />
</td><br />
</tr><br />
<br />
<tr style="height: 200px;" id="14"><br />
<td><br />
<img src="https://static.igem.org/mediawiki/2011/e/e3/Muradiye.jpg"/><br />
</td><br />
<td width="600" style="padding-left: 20px; padding-bottom: 20px;"><br />
<table style="margin-top:0px;"><br />
<tr><td><br />
<big>MURADİYE ACAR</big><br/>(Ph.D Student on Mecdical Genetics)<br />
</td></tr><br />
<tr><br />
<td><br />
&nbsp; </td><br />
</tr><br />
</table><br />
</td><br />
</tr><br />
<br />
<tr style="height: 70px;vertical-align: top;"><th colspan="2" style="text-align: center; font-size: 20px; font-weight: bold;">ATTRIBUTION</th></tr><br />
<td><br />
Canvas Town is a proud product of Fatih Turkey group where all work presented on this wiki is the sole work of the iGEM team members listed above.<br />
</td><br />
<br />
<br />
<tr style="height: 70px;vertical-align: top;"><th colspan="2" style="text-align: center; font-size: 20px; font-weight: bold;">STUDENTS</th></tr><br />
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<tr style="height: 200px;" id="1"><br />
<td><br />
<img src="https://static.igem.org/mediawiki/2011/0/0a/Alperen.jpg"/><br />
</td><br />
<td width="600" style="padding-left: 20px; padding-bottom: 20px;"><br />
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<big>ALPEREN GÜVER</big><br/>Medical Student (2nd Year)<br />
</td></tr><br />
<tr><br />
<td><br />
<p>I am a first term student of Fatih University Medical Faculty and I will finish first year on July. When I finish my faculty, I want to be a clinician. I like playing tennis and taking some photos.</p><br />
</td><br />
</tr><br />
</table><br />
</td><br />
</tr><br />
<br />
<tr style="height: 200px;" id="2"><br />
<td><br />
<img src="https://static.igem.org/mediawiki/2011/f/f7/Betul.jpg"/><br />
</td><br />
<td width="600" style="padding-left: 20px; padding-bottom: 20px;"><br />
<table style="margin-top:0px;"><br />
<tr><td><br />
<big>BETÜL GÜNBEY</big><br/>Medical Student (2nd Year)<br />
</td></tr><br />
<tr><br />
<td><br />
<p>I’m a first year student in medical faculty. Also this is my first year in iGEM, I’m interested in clinical medicine; but also, I always like genetics and working in the lab. I think iGEM is a perfect way to learn synthetic biology and teamwork for a student. I like playing guitar. I’m sure our team will be very successful.</p><br />
</td><br />
</tr><br />
</table><br />
</td><br />
</tr><br />
<br />
<tr style="height: 200px;" id="3"><br />
<td><br />
<img src="https://static.igem.org/mediawiki/2011/0/0f/Hasanyasin.jpg"/><br />
</td><br />
<td width="600" style="padding-left: 20px; padding-bottom: 20px;"><br />
<table style="margin-top:0px;"><br />
<tr><td><br />
<big>HASAN YASİN SOYLU</big><br/>Medical Student (2nd Year)<br />
</td></tr><br />
<tr><br />
<td><br />
<p>I'm happy to be a part of the team. There is nothing to say about the past time; but I'm here for the future.<br />
"Bismillah is the beginning of every good thing. So, let's say Bismillah!.."</p><br />
</td><br />
</tr><br />
</table><br />
</td><br />
</tr><br />
<br />
<tr style="height: 200px;" id="4"><br />
<td><br />
<img src="https://static.igem.org/mediawiki/2011/7/7a/Team-fazilet.jpg"/><br />
</td><br />
<td width="600" style="padding-left: 20px; padding-bottom: 20px;"><br />
<table style="margin-top:0px;"><br />
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<big>FAZİLET GÜLER</big><br/>Medical Student (3rd Year)<br />
</td></tr><br />
<tr><br />
<td><br />
<p>I finished my second year in medical school on June. I'll be a doctor but I don't want to be a clinician. Thus, genetics and synthetic biology are treasure for me:) This is my first year in iGEM. I'm interested in physics and computer science. I like traveling and hiking. Also, I enjoy riding a roller coaster!</p><br />
</td><br />
</tr><br />
</table><br />
</td><br />
</tr><br />
<br />
<tr style="height: 200px;" id="5"><br />
<td><br />
<img src="https://static.igem.org/mediawiki/2011/9/95/Kenangzl.jpg"/><br />
</td><br />
<td width="600" style="padding-left: 20px; padding-bottom: 20px;"><br />
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<big>KENAN OĞUZ GÜZEL</big><br/>Cartoonist<br />
</td></tr><br />
<tr><br />
<td><br />
&nbsp;<br />
</td><br />
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</table><br />
</td><br />
</tr><br />
<br />
<tr style="height: 200px;" id="6"><br />
<td><br />
<img src="https://static.igem.org/mediawiki/2011/d/d0/Hale.jpg"/><br />
</td><br />
<td width="600" style="padding-left: 20px; padding-bottom: 20px;"><br />
<table style="margin-top:0px;"><br />
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<big>HALENUR GÜNGÖRDÜ</big><br/>Medical Student (3rd Year)<br />
</td></tr><br />
<tr><br />
<td><br />
<p>I'm second term student of Fatih University Medical Faculty. This is the first time to attend iGEM competition. In spite of this, we have a great team and our advisors who spend a lot of time for that project. This competition process will be a great advantage for us like working together in solidarity and making idea exchange between us. So this project will broaden our horizon. See you in Holland(o'-'o)//</p><br />
</td><br />
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</td><br />
</tr><br />
<br />
<tr style="height: 200px; "id="7"><br />
<td><br />
<img src="https://static.igem.org/mediawiki/2011/f/ff/Mustafa.jpg"/><br />
</td><br />
<td width="600" style="padding-left: 20px; padding-bottom: 20px;"><br />
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<big>MUSTAFA SEMİH ELİTOK</big><br/>Medical Student (2nd Year)<br />
</td></tr><br />
<tr><br />
<td><br />
<p>I'm Mustafa, undergraduate student in Fatih University Medical School. First of all, being a part of this team is an honor. I am so impressed of their patience with me. I am definitely a laid-back person, because my point of view to the life is about not taking the things so seriously. I mean seeing the facts on their funny ways, especially when it gets complicated and seems hard. And the recent studies corrects me that not being stressed is healthy, so relax. And I like playing basketball, chess and poking other people.</p><br />
</td><br />
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</td><br />
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<td><br />
<img src="https://static.igem.org/mediawiki/2011/3/30/Secil.jpg"/><br />
</td><br />
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<big>SEÇİL TAŞHAN</big><br/>Medical Student (2nd Year)<br />
</td></tr><br />
<tr><br />
<td><br />
<p>This is my first year at Fatih University Medical School as a student. Although my department is the Medicine , I have a great interest in Genetics. After I complete my 6-year medical education , I want to maintain my academic education in Genetics. We are the first medical school in Turkey that participates in iGEM Competition. I hope we will maintain our success for next years, too.. I love watching cartoon :)</p><br />
</td><br />
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</td><br />
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<br />
<tr style="height: 200px;" id="9"><br />
<td><br />
<img src="https://static.igem.org/mediawiki/2011/8/81/Ozantahsin1.jpg"/><br />
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<big>OZAN SAMİ BAYRAK</big><br/>Medical Student (2nd Year)<br />
</td></tr><br />
<tr><br />
<td><br />
<p>I'm Ozan! I'm undergraduate student at Fatih University Medical School. I like genetics and synthetic biology, so I'm a part of this team. I advise you to participate in iGEM if you realy love genetics. In addition, I'm a goalkeeper and football is my life. If I didn't choose to be a doctor, I would be a professional goalkeeper.</p><br />
</td><br />
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<tr style="height: 200px;" id="10"><br />
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<big>TÜLİN SERAP YILMAZ</big><br/>Medical Student (2nd Year)<br />
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<td><br />
<p>Hi! I m Tülin Serap Yılmaz. I finished my first year in Fatih University Medical School .I m interested in genetics, surgery and psychiatry. I am too bored learning everything in books; so I took part in Team Genesis . We learn everything by seeing. I wish an enjoying summer full with science for you :)</p><br />
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<big>YASİN ÇAKAR</big><br/>Medical Student (2nd Year)<br />
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<p>Yasin Çakar.. My first year in medicine school.. Glad to being a part of experimental researches in genetics and being a member of Fatih University iGEM Team. Believing that it's loss if your today is equal to yesterday, tomorrow should be different from today..</p><br />
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<big>BURAK ŞİMŞEK</big><br/>Computer Engineering Student (4th Year)<br />
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<td><br />
&nbsp;<br />
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Metugen
http://2011.igem.org/Team:Fatih_Turkey/Team
Team:Fatih Turkey/Team
2011-09-22T02:22:25Z
<p>Metugen: </p>
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<h2 style="font-family: Verdana, Arial, SunSans-Regular, sans-serif;">Team</h2><br />
<h5 style="font-family: Verdana, Arial, SunSans-Regular, sans-serif;">All Members</h5> <br />
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<tr style="height: 70px; vertical-align: top;"><th colspan="2" style="text-align: center; font-size: 20px; font-weight: bold;">INSTRUCTORS</th></tr><br />
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<img src="https://static.igem.org/mediawiki/2011/6/64/Esrahoca.jpg"/><br />
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<tr><td><br />
<big>ESRA GÜNDÜZ</big><br/>(Associate Professor of Medical Genetics)<br />
</td></tr><br />
<tr><br />
<td><br />
<p>Hello everyone! My name is Esra. I’m an Associate Professor of Medical Genetics. This is my third year back in Turkey after staying twelve years in Japan. I mainly work on Cancer Genetics and more specifically on Tumor Suppressor Genes. I do enjoy being with my students and working with them together. iGEM competition has provided us to become closer. I think we share a lot scientifically, socially and friendly together with my students. I also like speaking Japanese and cooking.</p><br />
</td><br />
</tr><br />
</table><br />
</td><br />
</tr><br />
<br />
<tr style="height: 200px;" id="17"><br />
<td><br />
<img src="https://static.igem.org/mediawiki/2011/6/64/Kadirhoca.jpg"/><br />
</td><br />
<td width="600" style="padding-left: 20px; padding-bottom: 20px;"><br />
<table style="margin-top:0px;"><br />
<tr><td><br />
<big>KADİR DEMİRCAN</big><br/>(Assistant Professor of Medical Genetics)<br />
</td></tr><br />
<tr><br />
<td>&nbsp;<br />
</td><br />
</tr><br />
</table><br />
</td><br />
</tr><br />
<br />
<br />
<tr style="height: 200px;" id="16"><br />
<td><br />
<img src="https://static.igem.org/mediawiki/2011/b/b2/Husamettinhoca.jpg"/><br />
</td><br />
<td width="600" style="padding-left: 20px; padding-bottom: 20px;"><br />
<table style="margin-top:0px;"><br />
<tr><td><br />
<big>HÜSAMETTİN ERDAMAR</big><br/>(Assistant Professor of Medical Biochemistry)<br />
</td></tr><br />
<tr><br />
<td>&nbsp;</td><br />
</tr><br />
</table><br />
</td><br />
<br />
</tr><br />
<br />
<tr style="height: 70px;vertical-align: top;"><th colspan="2" style="text-align: center; font-size: 20px; font-weight: bold;">ADVISORS</th></tr><br />
<br />
<tr style="height: 200px;" id="13"><br />
<td><br />
<img src="https://static.igem.org/mediawiki/2011/c/c1/Cihantastan.jpg"/><br />
</td><br />
<td width="600" style="padding-left: 20px; padding-bottom: 20px;"><br />
<table style="margin-top:0px;"><br />
<tr><td><br />
<big>CİHAN TAŞTAN</big><br/>(Ph.D Student on Tumor Immunology at Sackler Institue of New York University)<br />
</td></tr><br />
<tr><br />
<td><br />
<p> I am graduate student for Ph.D. on Tumor Immunology at Sackler Institue of New York University. I have participated iGEM competition several times. I have taken parts in the competition as a student member of METU-Gene 2009 (Silver), METU-Turkey Software 2010 (Silver) and METU-Turkey 2010 (Gold). Now, I have been advisor of Fatih University for couple of months in order to start integration of other universities of Turkey into iGEM and to prepare an environment for establisihing and spreading of new synthetic biology labs through Turkey.</p><br />
</td><br />
</tr><br />
</table><br />
</td><br />
</tr><br />
<br />
<tr style="height: 200px;" id="14"><br />
<td><br />
<img src="https://static.igem.org/mediawiki/2011/e/e3/Muradiye.jpg"/><br />
</td><br />
<td width="600" style="padding-left: 20px; padding-bottom: 20px;"><br />
<table style="margin-top:0px;"><br />
<tr><td><br />
<big>MURADİYE ACAR</big><br/>(Ph.D Student on Mecdical Genetics)<br />
</td></tr><br />
<tr><br />
<td><br />
&nbsp; </td><br />
</tr><br />
</table><br />
</td><br />
</tr><br />
<br />
<tr style="height: 70px;vertical-align: top;"><th colspan="2" style="text-align: center; font-size: 20px; font-weight: bold;">ATTRIBUTION</th></tr><br />
<td><br />
<p>Canvas Town is a proud product of Fatih Turkey group where all work presented on this wiki is the sole work of the iGEM team members listed above. </p><br />
</td><br />
<br />
<br />
<tr style="height: 70px;vertical-align: top;"><th colspan="2" style="text-align: center; font-size: 20px; font-weight: bold;">STUDENTS</th></tr><br />
<br />
<tr style="height: 200px;" id="1"><br />
<td><br />
<img src="https://static.igem.org/mediawiki/2011/0/0a/Alperen.jpg"/><br />
</td><br />
<td width="600" style="padding-left: 20px; padding-bottom: 20px;"><br />
<table style="margin-top:0px;"><br />
<tr><td><br />
<big>ALPEREN GÜVER</big><br/>Medical Student (2nd Year)<br />
</td></tr><br />
<tr><br />
<td><br />
<p>I am a first term student of Fatih University Medical Faculty and I will finish first year on July. When I finish my faculty, I want to be a clinician. I like playing tennis and taking some photos.</p><br />
</td><br />
</tr><br />
</table><br />
</td><br />
</tr><br />
<br />
<tr style="height: 200px;" id="2"><br />
<td><br />
<img src="https://static.igem.org/mediawiki/2011/f/f7/Betul.jpg"/><br />
</td><br />
<td width="600" style="padding-left: 20px; padding-bottom: 20px;"><br />
<table style="margin-top:0px;"><br />
<tr><td><br />
<big>BETÜL GÜNBEY</big><br/>Medical Student (2nd Year)<br />
</td></tr><br />
<tr><br />
<td><br />
<p>I’m a first year student in medical faculty. Also this is my first year in iGEM, I’m interested in clinical medicine; but also, I always like genetics and working in the lab. I think iGEM is a perfect way to learn synthetic biology and teamwork for a student. I like playing guitar. I’m sure our team will be very successful.</p><br />
</td><br />
</tr><br />
</table><br />
</td><br />
</tr><br />
<br />
<tr style="height: 200px;" id="3"><br />
<td><br />
<img src="https://static.igem.org/mediawiki/2011/0/0f/Hasanyasin.jpg"/><br />
</td><br />
<td width="600" style="padding-left: 20px; padding-bottom: 20px;"><br />
<table style="margin-top:0px;"><br />
<tr><td><br />
<big>HASAN YASİN SOYLU</big><br/>Medical Student (2nd Year)<br />
</td></tr><br />
<tr><br />
<td><br />
<p>I'm happy to be a part of the team. There is nothing to say about the past time; but I'm here for the future.<br />
"Bismillah is the beginning of every good thing. So, let's say Bismillah!.."</p><br />
</td><br />
</tr><br />
</table><br />
</td><br />
</tr><br />
<br />
<tr style="height: 200px;" id="4"><br />
<td><br />
<img src="https://static.igem.org/mediawiki/2011/7/7a/Team-fazilet.jpg"/><br />
</td><br />
<td width="600" style="padding-left: 20px; padding-bottom: 20px;"><br />
<table style="margin-top:0px;"><br />
<tr><td><br />
<big>FAZİLET GÜLER</big><br/>Medical Student (3rd Year)<br />
</td></tr><br />
<tr><br />
<td><br />
<p>I finished my second year in medical school on June. I'll be a doctor but I don't want to be a clinician. Thus, genetics and synthetic biology are treasure for me:) This is my first year in iGEM. I'm interested in physics and computer science. I like traveling and hiking. Also, I enjoy riding a roller coaster!</p><br />
</td><br />
</tr><br />
</table><br />
</td><br />
</tr><br />
<br />
<tr style="height: 200px;" id="5"><br />
<td><br />
<img src="https://static.igem.org/mediawiki/2011/9/95/Kenangzl.jpg"/><br />
</td><br />
<td width="600" style="padding-left: 20px; padding-bottom: 20px;"><br />
<table style="margin-top:0px;"><br />
<tr><td><br />
<big>KENAN OĞUZ GÜZEL</big><br/>Cartoonist<br />
</td></tr><br />
<tr><br />
<td><br />
&nbsp;<br />
</td><br />
</tr><br />
</table><br />
</td><br />
</tr><br />
<br />
<tr style="height: 200px;" id="6"><br />
<td><br />
<img src="https://static.igem.org/mediawiki/2011/d/d0/Hale.jpg"/><br />
</td><br />
<td width="600" style="padding-left: 20px; padding-bottom: 20px;"><br />
<table style="margin-top:0px;"><br />
<tr><td><br />
<big>HALENUR GÜNGÖRDÜ</big><br/>Medical Student (3rd Year)<br />
</td></tr><br />
<tr><br />
<td><br />
<p>I'm second term student of Fatih University Medical Faculty. This is the first time to attend iGEM competition. In spite of this, we have a great team and our advisors who spend a lot of time for that project. This competition process will be a great advantage for us like working together in solidarity and making idea exchange between us. So this project will broaden our horizon. See you in Holland(o'-'o)//</p><br />
</td><br />
</tr><br />
</table><br />
</td><br />
</tr><br />
<br />
<tr style="height: 200px; "id="7"><br />
<td><br />
<img src="https://static.igem.org/mediawiki/2011/f/ff/Mustafa.jpg"/><br />
</td><br />
<td width="600" style="padding-left: 20px; padding-bottom: 20px;"><br />
<table style="margin-top:0px;"><br />
<tr><td><br />
<big>MUSTAFA SEMİH ELİTOK</big><br/>Medical Student (2nd Year)<br />
</td></tr><br />
<tr><br />
<td><br />
<p>I'm Mustafa, undergraduate student in Fatih University Medical School. First of all, being a part of this team is an honor. I am so impressed of their patience with me. I am definitely a laid-back person, because my point of view to the life is about not taking the things so seriously. I mean seeing the facts on their funny ways, especially when it gets complicated and seems hard. And the recent studies corrects me that not being stressed is healthy, so relax. And I like playing basketball, chess and poking other people.</p><br />
</td><br />
</tr><br />
</table><br />
</td><br />
</tr><br />
<br />
<tr style="height: 200px;" id="8"><br />
<td><br />
<img src="https://static.igem.org/mediawiki/2011/3/30/Secil.jpg"/><br />
</td><br />
<td width="600" style="padding-left: 20px; padding-bottom: 20px;"><br />
<table style="margin-top:0px;"><br />
<tr><td><br />
<big>SEÇİL TAŞHAN</big><br/>Medical Student (2nd Year)<br />
</td></tr><br />
<tr><br />
<td><br />
<p>This is my first year at Fatih University Medical School as a student. Although my department is the Medicine , I have a great interest in Genetics. After I complete my 6-year medical education , I want to maintain my academic education in Genetics. We are the first medical school in Turkey that participates in iGEM Competition. I hope we will maintain our success for next years, too.. I love watching cartoon :)</p><br />
</td><br />
</tr><br />
</table><br />
</td><br />
</tr><br />
<br />
<tr style="height: 200px;" id="9"><br />
<td><br />
<img src="https://static.igem.org/mediawiki/2011/8/81/Ozantahsin1.jpg"/><br />
</td><br />
<td width="600" style="padding-left: 20px; padding-bottom: 20px;"><br />
<table style="margin-top:0px;"><br />
<tr><td><br />
<big>OZAN SAMİ BAYRAK</big><br/>Medical Student (2nd Year)<br />
</td></tr><br />
<tr><br />
<td><br />
<p>I'm Ozan! I'm undergraduate student at Fatih University Medical School. I like genetics and synthetic biology, so I'm a part of this team. I advise you to participate in iGEM if you realy love genetics. In addition, I'm a goalkeeper and football is my life. If I didn't choose to be a doctor, I would be a professional goalkeeper.</p><br />
</td><br />
</tr><br />
</table><br />
</td><br />
</tr><br />
<br />
<tr style="height: 200px;" id="10"><br />
<td><br />
<img src="https://static.igem.org/mediawiki/2011/7/72/Tserap1.jpg"/><br />
</td><br />
<td width="600" style="padding-left: 20px; padding-bottom: 20px;"><br />
<table style="margin-top:0px;"><br />
<tr><td><br />
<big>TÜLİN SERAP YILMAZ</big><br/>Medical Student (2nd Year)<br />
</td></tr><br />
<tr><br />
<td><br />
<p>Hi! I m Tülin Serap Yılmaz. I finished my first year in Fatih University Medical School .I m interested in genetics, surgery and psychiatry. I am too bored learning everything in books; so I took part in Team Genesis . We learn everything by seeing. I wish an enjoying summer full with science for you :)</p><br />
</td><br />
</tr><br />
</table><br />
</td><br />
</tr><br />
<br />
<tr style="height: 200px;" id="11"><br />
<td><br />
<img src="https://static.igem.org/mediawiki/2011/a/a3/Yasincakar.jpg"/><br />
</td><br />
<td width="600" style="padding-left: 20px; padding-bottom: 20px;"><br />
<table style="margin-top:0px;"><br />
<tr><td><br />
<big>YASİN ÇAKAR</big><br/>Medical Student (2nd Year)<br />
</td></tr><br />
<tr><br />
<td><br />
<p>Yasin Çakar.. My first year in medicine school.. Glad to being a part of experimental researches in genetics and being a member of Fatih University iGEM Team. Believing that it's loss if your today is equal to yesterday, tomorrow should be different from today..</p><br />
</td><br />
</tr><br />
</table><br />
</td><br />
</tr><br />
<br />
<tr style="height: 200px;" id="12"><br />
<td><br />
<img src="https://static.igem.org/mediawiki/2011/8/83/Buraksimsek.jpg"/><br />
</td><br />
<td width="600" style="padding-left: 20px; padding-bottom: 20px;"><br />
<table style="margin-top:0px;"><br />
<tr><td><br />
<big>BURAK ŞİMŞEK</big><br/>Computer Engineering Student (4th Year)<br />
</td></tr><br />
<tr><br />
<td><br />
&nbsp;<br />
</td><br />
</tr><br />
</table><br />
</td><br />
</tr><br />
<br />
</table><br />
</center><br />
</div><br />
</div><br />
</div><br />
</div><br />
</div><br />
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<div class="footer-sh-left"></div><br />
<div class="footer-sh"></div><br />
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</div><br />
<div id="footer"><br />
<style><br />
#footer img:hover{<br />
opacity:0.6;<br />
}<br />
</style><br />
<div><br />
<h3 style="color:#BBB;">Sponsors</h3><br />
<img height="100" src="https://static.igem.org/mediawiki/2011/7/72/Tubitak_-_Kopya.png"/><br />
<a href="http://www.sentegen.com"><br />
<img height="100" src="https://static.igem.org/mediawiki/2011/2/2d/Sentegennnn.png"/></a><br />
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2011 © Fatih Medical School<br />
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Metugen
http://2011.igem.org/Team:Fatih_Turkey/Human_Practice
Team:Fatih Turkey/Human Practice
2011-09-22T01:36:05Z
<p>Metugen: </p>
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<h2 style="font-family: Verdana, Arial, SunSans-Regular, sans-serif;">Human Practice</h2><br />
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<td><h2><a href="https://2011.igem.org/Team:Fatih_Turkey/Sporocide">Sporocide</a></h2></td><br />
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<tr><td>To remove infections B.subtilis spores from our Anti-LPS factor containing biofilm paste, fenton reagent is used before use against E.coli.</td></tr><br />
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<tr><td>During funny summer in our project executions, we have also met hundreds of people from different ages in order to tell our project in iGEM competition and benefits of synthetic biology.</td></tr><br />
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<tr><td>We have designed monopoly like game name "The Canvas Town" to inform people from any distinct education level about features of our project, laboratory equipments and interesting Biobrick of iGEM competition.</td></tr><br />
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<tr><td>a new journal of young scientists; it gives you the actual news of our lab and team</td></tr><br />
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<br />
<h1>Outline and Detailed Human Practice</h1></div><br />
<br />
<div> <br />
<tr><td>During our Human Practice studies, we have tried to tell our project, its usage areas, synthetic better life not only to our medical student friends coming from different schools through Turkey but also citizens, childrens, highschool students <strong>with the help of `Canvas Town` our novel board game, `iGEM for 7 to 77` our weekly open-lab to the public meetings and our `Canvas Times` great summer magazine containing all our human practice activities. </strong><br />
<br />
<div>To be able to reach the best human practice prize;</tr></td></div> <br />
<br />
<div> <br />
<tr><td>1) Firstly, we aimed in our project, obtain an anti gram negative surface with using B. subtilis and E.coli bacteria. E. coli is killing by B. subtilis with B. subtilis’s own new protein LALF. But B. subtilis is a gram positive and this surface can’t protect us from some pathogenic effects of B.Subtilis’s spores. For protecting from B. subtilis we used <strong>the sporocide method as a non-used B.subtilis procedure in iGEM</strong> useful for further B.subtilis synthetic biology projects. Fenton Reagent in Sporocide methode is making our project more safety and useful as wanted in Human practice safety issue approaching way of our project. </tr></td></div> <br />
<br />
<div><tr><td>2) Secondly we have informed people about our project, synthetic biology and iGEM competition, and draw their attention to genetics.</td></tr></div><br />
<br />
<div><tr><td>- We prepared <strong>a board game named “The Canvas Town”</strong> to inform people from any distinct education level about features of our project, laboratory equipments and interesting Biobrick parts of iGEM competıtion. We have played several times with lots of people some of them are a group of gifted children and the Turkey’s other iGEM teams and it was very funny and informative. </tr></td></div><br />
<br />
<div><tr><td>-Our one of the most important goals is to introduce iGEM and Synthetic Biology to the students in Turkey. But we did not want to limit ourselves by choosing only same age groups and we asked to several ages of people <strong>“what do you want from your bacteria to do for you?” </strong>With this question we went to a kindergarten and we gave very interesting answers. The children were very interested about genetics, synthetic biology and microorganisms. Also we asked the same question to students of our university and the ideas were very funny and useful for synthetic biology. With these activities we drew their attention to genetics, synthetic biology and iGEM. </td></tr></div><br />
<br />
<div><tr><td>-<strong>Second International Medical Student Conference in Ankara</strong> had performed on April 2011 and it’s organized by University of Fatih Medical Faculty. In the conference, synthetic biology and iGEM competition related presentation was performed to inform medical school students coming from different medical schools in Turkey and also from out of Turkey ie. Japan.</td></tr></div><br />
<br />
<div><tr><td>3) Thirdly we have showed people that the benefits of synthetic biology and microorganisms </div><br />
<br />
<div>-We strove for the innovation and presentation of synthetic biology to people. As a medical school, our hospital has its <strong>own quarterly magazine, “Yasama Sanati” (in English: The Art of Living)</strong>. We decided that we could mention about iGEM and synthetic biology; thus we wrote an article that told the bacteria is not too much harmful living creature to humans as it is seen nowadays. In fact, we mentioned that bacteria would become very handy, helpful and intelligent “machines” in appropriate conditions. We listed the former experiments and parts that took place in iGEM competition and gave some examples about what to do next. With this article lots of people informed and their some negative sentiments about the microorganisms and synthetic biology would be changed.</td></tr></div><br />
<br />
<div><tr><td>4) We have prepared <strong>a journal which is named “Canvas Times” </strong>and it gives you the actual news of our lab and team. Also you can learn about our project and read our columnist and you can travel our peace and quiet town the Canvas Town. “Canvas Times” a new journal of young scientists; it gives you the actual news of our lab and team. <br />
During our lab working, we could find some time to fun and talk with people about our project, synthetic biology and iGEM... Our all of activities and human practices are existed on our journal such as<strong> “iGEM Picnic” and “International Medical Students Conference</strong>. Therewithal you will find some information of our university and faculty. You can find it all of stores.</div><br />
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Metugen
http://2011.igem.org/Team:Fatih_Turkey/Human_Practice
Team:Fatih Turkey/Human Practice
2011-09-22T01:34:20Z
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<h2 style="font-family: Verdana, Arial, SunSans-Regular, sans-serif;">Human Practice</h2><br />
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<td><h2><a href="https://2011.igem.org/Team:Fatih_Turkey/Sporocide">Sporocide</a></h2></td><br />
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<tr><td>To remove infections B.subtilis spores from our Anti-LPS factor containing biofilm paste, fenton reagent is used before use against E.coli.</td></tr><br />
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<tr><td>During funny summer in our project executions, we have also met hundreds of people from different ages in order to tell our project in iGEM competition and benefits of synthetic biology.</td></tr><br />
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<tr><td>We have designed monopoly like game name "The Canvas Town" to inform people from any distinct education level about features of our project, laboratory equipments and interesting Biobrick of iGEM competition.</td></tr><br />
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<tr><td>a new journal of young scientists; it gives you the actual news of our lab and team</td></tr><br />
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<br />
<h1>Outline and Detailed Human Practice</h1></div><br />
<br />
<div> <br />
<tr><td>During our Human Practice studies, we have tried to tell our project, its usage areas, synthetic better life not only to our medical student friends coming from different schools through Turkey but also citizens, childrens, highschool students <strong>with the help of `Canvas Town` our novel board game, `iGEM for 7 to 77` our weekly open-lab to the public meetings and our `Canvas Times` great summer magazine containing all our human practice activities. </strong><br />
<br />
<div>To be able to reach the best human practice prize;</tr></td></div> <br />
<br />
<div> <br />
<tr><td>1) Firstly, we aimed in our project, obtain an anti gram negative surface with using B. subtilis and E.coli bacteria. E. coli is killing by B. subtilis with B. subtilis’s own new protein LALF. But B. subtilis is a gram positive and this surface can’t protect us from some pathogenic effects of B.Subtilis’s spores. For protecting from B. subtilis we used the sporocide method as a non-used B.subtilis procedure in iGEM useful for further B.subtilis synthetic biology projects. Fenton Reagent in Sporocide methode is making our project more safety and useful as wanted in Human practice safety issue approaching way of our project. </tr></td></div> <br />
<br />
<div><tr><td>2) Secondly we have informed people about our project, synthetic biology and iGEM competition, and draw their attention to genetics.</td></tr></div><br />
<br />
<div><tr><td>- We prepared a board game named “The Canvas Town” to inform people from any distinct education level about features of our project, laboratory equipments and interesting Biobrick parts of iGEM competıtion. We have played several times with lots of people some of them are a group of gifted children and the Turkey’s other iGEM teams and it was very funny and informative. </tr></td></div><br />
<br />
<div><tr><td>-Our one of the most important goals is to introduce iGEM and Synthetic Biology to the students in Turkey. But we did not want to limit ourselves by choosing only same age groups and we asked to several ages of people “what do you want from your bacteria to do for you?” With this question we went to a kindergarten and we gave very interesting answers. The children were very interested about genetics, synthetic biology and microorganisms. Also we asked the same question to students of our university and the ideas were very funny and useful for synthetic biology. With these activities we drew their attention to genetics, synthetic biology and iGEM. </td></tr></div><br />
<br />
<div><tr><td>-Second International Medical Student Conference in Ankara had performed on April 2011 and it’s organized by University of Fatih Medical Faculty. In the conference, synthetic biology and iGEM competition related presentation was performed to inform medical school students coming from different medical schools in Turkey and also from out of Turkey ie. Japan.</td></tr></div><br />
<br />
<div><tr><td>3) Thirdly we have showed people that the benefits of synthetic biology and microorganisms </div><br />
<br />
<div>-We strove for the innovation and presentation of synthetic biology to people. As a medical school, our hospital has its own quarterly magazine, “Yasama Sanati” (in English: The Art of Living). We decided that we could mention about iGEM and synthetic biology; thus we wrote an article that told the bacteria is not too much harmful living creature to humans as it is seen nowadays. In fact, we mentioned that bacteria would become very handy, helpful and intelligent “machines” in appropriate conditions. We listed the former experiments and parts that took place in iGEM competition and gave some examples about what to do next. With this article lots of people informed and their some negative sentiments about the microorganisms and synthetic biology would be changed.</td></tr></div><br />
<br />
<div><tr><td>4) We have prepared a journal which is named “Canvas Times” and it gives you the actual news of our lab and team. Also you can learn about our project and read our columnist and you can travel our peace and quiet town the Canvas Town. “Canvas Times” a new journal of young scientists; it gives you the actual news of our lab and team. <br />
During our lab working, we could find some time to fun and talk with people about our project, synthetic biology and iGEM... Our all of activities and human practices are existed on our journal such as “iGEM Picnic” and “International Medical Students Conference. Therewithal you will find some information of our university and faculty. You can find it all of stores.</div><br />
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Metugen
http://2011.igem.org/Team:Fatih_Turkey/Human_Practice
Team:Fatih Turkey/Human Practice
2011-09-22T01:33:57Z
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<a href="https://2011.igem.org/Team:Fatih_Turkey/Project">Project<span>Rainbow Graveyard</span></a><br />
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<h2 style="font-family: Verdana, Arial, SunSans-Regular, sans-serif;">Human Practice</h2><br />
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<a href="https://2011.igem.org/Team:Fatih_Turkey/Sporocide"><img src="https://static.igem.org/mediawiki/2011/2/24/Sporocideicon.jpg" width="160" /></a><br />
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<td><h2><a href="https://2011.igem.org/Team:Fatih_Turkey/Sporocide">Sporocide</a></h2></td><br />
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<tr><td>To remove infections B.subtilis spores from our Anti-LPS factor containing biofilm paste, fenton reagent is used before use against E.coli.</td></tr><br />
</td><br />
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<td><h2><a href="https://2011.igem.org/Team:Fatih_Turkey/iGEM_for_7_to_77">iGEM for 7 to 77</a></h2></td><br />
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<tr><td>During funny summer in our project executions, we have also met hundreds of people from different ages in order to tell our project in iGEM competition and benefits of synthetic biology.</td></tr><br />
</td><br />
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<td><h2><a href="https://2011.igem.org/Team:Fatih_Turkey/game">Game</a></h2></td><br />
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<tr><td>We have designed monopoly like game name "The Canvas Town" to inform people from any distinct education level about features of our project, laboratory equipments and interesting Biobrick of iGEM competition.</td></tr><br />
</td><br />
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<td><h2><a href="https://2011.igem.org/Team:Fatih_Turkey/canvas_times">Canvas Times</a></h2></td><br />
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<tr><td>a new journal of young scientists; it gives you the actual news of our lab and team</td></tr><br />
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<br />
<h1>Outline and Detailed Human Practice</h1></div><br />
<br />
<div> <br />
<tr><td>During our Human Practice studies, we have tried to tell our project, its usage areas, synthetic better life not only to our medical student friends coming from different schools through Turkey but also citizens, childrens, highschool students <p><strong>with the help of `Canvas Town` our novel board game, `iGEM for 7 to 77` our weekly open-lab to the public meetings and our `Canvas Times` great summer magazine containing all our human practice activities. </strong></p><br />
<br />
<div>To be able to reach the best human practice prize;</tr></td></div> <br />
<br />
<div> <br />
<tr><td>1) Firstly, we aimed in our project, obtain an anti gram negative surface with using B. subtilis and E.coli bacteria. E. coli is killing by B. subtilis with B. subtilis’s own new protein LALF. But B. subtilis is a gram positive and this surface can’t protect us from some pathogenic effects of B.Subtilis’s spores. For protecting from B. subtilis we used the sporocide method as a non-used B.subtilis procedure in iGEM useful for further B.subtilis synthetic biology projects. Fenton Reagent in Sporocide methode is making our project more safety and useful as wanted in Human practice safety issue approaching way of our project. </tr></td></div> <br />
<br />
<div><tr><td>2) Secondly we have informed people about our project, synthetic biology and iGEM competition, and draw their attention to genetics.</td></tr></div><br />
<br />
<div><tr><td>- We prepared a board game named “The Canvas Town” to inform people from any distinct education level about features of our project, laboratory equipments and interesting Biobrick parts of iGEM competıtion. We have played several times with lots of people some of them are a group of gifted children and the Turkey’s other iGEM teams and it was very funny and informative. </tr></td></div><br />
<br />
<div><tr><td>-Our one of the most important goals is to introduce iGEM and Synthetic Biology to the students in Turkey. But we did not want to limit ourselves by choosing only same age groups and we asked to several ages of people “what do you want from your bacteria to do for you?” With this question we went to a kindergarten and we gave very interesting answers. The children were very interested about genetics, synthetic biology and microorganisms. Also we asked the same question to students of our university and the ideas were very funny and useful for synthetic biology. With these activities we drew their attention to genetics, synthetic biology and iGEM. </td></tr></div><br />
<br />
<div><tr><td>-Second International Medical Student Conference in Ankara had performed on April 2011 and it’s organized by University of Fatih Medical Faculty. In the conference, synthetic biology and iGEM competition related presentation was performed to inform medical school students coming from different medical schools in Turkey and also from out of Turkey ie. Japan.</td></tr></div><br />
<br />
<div><tr><td>3) Thirdly we have showed people that the benefits of synthetic biology and microorganisms </div><br />
<br />
<div>-We strove for the innovation and presentation of synthetic biology to people. As a medical school, our hospital has its own quarterly magazine, “Yasama Sanati” (in English: The Art of Living). We decided that we could mention about iGEM and synthetic biology; thus we wrote an article that told the bacteria is not too much harmful living creature to humans as it is seen nowadays. In fact, we mentioned that bacteria would become very handy, helpful and intelligent “machines” in appropriate conditions. We listed the former experiments and parts that took place in iGEM competition and gave some examples about what to do next. With this article lots of people informed and their some negative sentiments about the microorganisms and synthetic biology would be changed.</td></tr></div><br />
<br />
<div><tr><td>4) We have prepared a journal which is named “Canvas Times” and it gives you the actual news of our lab and team. Also you can learn about our project and read our columnist and you can travel our peace and quiet town the Canvas Town. “Canvas Times” a new journal of young scientists; it gives you the actual news of our lab and team. <br />
During our lab working, we could find some time to fun and talk with people about our project, synthetic biology and iGEM... Our all of activities and human practices are existed on our journal such as “iGEM Picnic” and “International Medical Students Conference. Therewithal you will find some information of our university and faculty. You can find it all of stores.</div><br />
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Metugen
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Team:Fatih Turkey/Human Practice
2011-09-22T01:33:35Z
<p>Metugen: </p>
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<tr><td>To remove infections B.subtilis spores from our Anti-LPS factor containing biofilm paste, fenton reagent is used before use against E.coli.</td></tr><br />
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<tr><td>During funny summer in our project executions, we have also met hundreds of people from different ages in order to tell our project in iGEM competition and benefits of synthetic biology.</td></tr><br />
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<td><h2><a href="https://2011.igem.org/Team:Fatih_Turkey/game">Game</a></h2></td><br />
</tr><br />
<tr><td>We have designed monopoly like game name "The Canvas Town" to inform people from any distinct education level about features of our project, laboratory equipments and interesting Biobrick of iGEM competition.</td></tr><br />
</td><br />
</tr><br />
</table><br />
</td><br />
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<a href="https://2011.igem.org/Team:Fatih_Turkey/canvas_times"><img src="https://static.igem.org/mediawiki/2011/0/05/Canvasicon.jpg" width="160" /></a><br />
</td><br />
<td width="600" style="vertical-align:top;"><br />
<table><br />
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<td><h2><a href="https://2011.igem.org/Team:Fatih_Turkey/canvas_times">Canvas Times</a></h2></td><br />
</tr><br />
<tr><td>a new journal of young scientists; it gives you the actual news of our lab and team</td></tr><br />
</td><br />
</tr><br />
</table><br />
</td><br />
</tr><br />
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<div align=`center`><br />
<br />
<h1>Outline and Detailed Human Practice</h1></div><br />
<br />
<div> <br />
<tr><td>During our Human Practice studies, we have tried to tell our project, its usage areas, synthetic better life not only to our medical student friends coming from different schools through Turkey but also citizens, childrens, highschool students <p><strong><span style="text-decoration: underline;">with the help of `Canvas Town` our novel board game, `iGEM for 7 to 77` our weekly open-lab to the public meetings and our `Canvas Times` great summer magazine containing all our human practice activities. </span></strong></p><br />
<br />
<div>To be able to reach the best human practice prize;</tr></td></div> <br />
<br />
<div> <br />
<tr><td>1) Firstly, we aimed in our project, obtain an anti gram negative surface with using B. subtilis and E.coli bacteria. E. coli is killing by B. subtilis with B. subtilis’s own new protein LALF. But B. subtilis is a gram positive and this surface can’t protect us from some pathogenic effects of B.Subtilis’s spores. For protecting from B. subtilis we used the sporocide method as a non-used B.subtilis procedure in iGEM useful for further B.subtilis synthetic biology projects. Fenton Reagent in Sporocide methode is making our project more safety and useful as wanted in Human practice safety issue approaching way of our project. </tr></td></div> <br />
<br />
<div><tr><td>2) Secondly we have informed people about our project, synthetic biology and iGEM competition, and draw their attention to genetics.</td></tr></div><br />
<br />
<div><tr><td>- We prepared a board game named “The Canvas Town” to inform people from any distinct education level about features of our project, laboratory equipments and interesting Biobrick parts of iGEM competıtion. We have played several times with lots of people some of them are a group of gifted children and the Turkey’s other iGEM teams and it was very funny and informative. </tr></td></div><br />
<br />
<div><tr><td>-Our one of the most important goals is to introduce iGEM and Synthetic Biology to the students in Turkey. But we did not want to limit ourselves by choosing only same age groups and we asked to several ages of people “what do you want from your bacteria to do for you?” With this question we went to a kindergarten and we gave very interesting answers. The children were very interested about genetics, synthetic biology and microorganisms. Also we asked the same question to students of our university and the ideas were very funny and useful for synthetic biology. With these activities we drew their attention to genetics, synthetic biology and iGEM. </td></tr></div><br />
<br />
<div><tr><td>-Second International Medical Student Conference in Ankara had performed on April 2011 and it’s organized by University of Fatih Medical Faculty. In the conference, synthetic biology and iGEM competition related presentation was performed to inform medical school students coming from different medical schools in Turkey and also from out of Turkey ie. Japan.</td></tr></div><br />
<br />
<div><tr><td>3) Thirdly we have showed people that the benefits of synthetic biology and microorganisms </div><br />
<br />
<div>-We strove for the innovation and presentation of synthetic biology to people. As a medical school, our hospital has its own quarterly magazine, “Yasama Sanati” (in English: The Art of Living). We decided that we could mention about iGEM and synthetic biology; thus we wrote an article that told the bacteria is not too much harmful living creature to humans as it is seen nowadays. In fact, we mentioned that bacteria would become very handy, helpful and intelligent “machines” in appropriate conditions. We listed the former experiments and parts that took place in iGEM competition and gave some examples about what to do next. With this article lots of people informed and their some negative sentiments about the microorganisms and synthetic biology would be changed.</td></tr></div><br />
<br />
<div><tr><td>4) We have prepared a journal which is named “Canvas Times” and it gives you the actual news of our lab and team. Also you can learn about our project and read our columnist and you can travel our peace and quiet town the Canvas Town. “Canvas Times” a new journal of young scientists; it gives you the actual news of our lab and team. <br />
During our lab working, we could find some time to fun and talk with people about our project, synthetic biology and iGEM... Our all of activities and human practices are existed on our journal such as “iGEM Picnic” and “International Medical Students Conference. Therewithal you will find some information of our university and faculty. You can find it all of stores.</div><br />
</td></tr><br />
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Team:Fatih Turkey/Human Practice
2011-09-22T01:31:11Z
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<li><a href="https://2011.igem.org/Team:Fatih_Turkey/game">Game</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/canvas_times">Canvas Times</a></li><br />
</ul><br />
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<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Notebook">Notebook</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Procedures">Procedures</a></li><br />
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<h2 style="font-family: Verdana, Arial, SunSans-Regular, sans-serif;">Human Practice</h2><br />
<h5 style="font-family: Verdana, Arial, SunSans-Regular, sans-serif;"></h5> <br />
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<a href="https://2011.igem.org/Team:Fatih_Turkey/Sporocide"><img src="https://static.igem.org/mediawiki/2011/2/24/Sporocideicon.jpg" width="160" /></a><br />
</td><br />
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<td><h2><a href="https://2011.igem.org/Team:Fatih_Turkey/Sporocide">Sporocide</a></h2></td><br />
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<tr><td>To remove infections B.subtilis spores from our Anti-LPS factor containing biofilm paste, fenton reagent is used before use against E.coli.</td></tr><br />
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<a href="https://2011.igem.org/Team:Fatih_Turkey/iGEM_for_7_to_77"><img src="https://static.igem.org/mediawiki/2011/7/75/Secilanaokuluicon.jpg" width="160" /></a><br />
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<td><h2><a href="https://2011.igem.org/Team:Fatih_Turkey/iGEM_for_7_to_77">iGEM for 7 to 77</a></h2></td><br />
</tr><br />
<tr><td>During funny summer in our project executions, we have also met hundreds of people from different ages in order to tell our project in iGEM competition and benefits of synthetic biology.</td></tr><br />
</td><br />
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<a href="https://2011.igem.org/Team:Fatih_Turkey/game"><img src="https://static.igem.org/mediawiki/2011/1/1b/Oyunicon.jpg" width="160" /></a><br />
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<td><h2><a href="https://2011.igem.org/Team:Fatih_Turkey/game">Game</a></h2></td><br />
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<tr><td>We have designed monopoly like game name "The Canvas Town" to inform people from any distinct education level about features of our project, laboratory equipments and interesting Biobrick of iGEM competition.</td></tr><br />
</td><br />
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<td><h2><a href="https://2011.igem.org/Team:Fatih_Turkey/canvas_times">Canvas Times</a></h2></td><br />
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<tr><td>a new journal of young scientists; it gives you the actual news of our lab and team</td></tr><br />
</td><br />
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<div align=`center`><br />
<br />
<h1>Outline and Detailed Human Practice</h1></div><br />
<br />
<div> <br />
<tr><td>During our Human Practice studies, we have tried to tell our project, its usage areas, synthetic better life not only to our medical student friends coming from different schools through Turkey but also citizens, childrens, highschool students with the help of `Canvas Town` our novel board game, `iGEM for 7 to 77` our weekly open-lab to the public meetings and our `Canvas Times` great summer magazine containing all our human practice activities. <br />
To be able to reach the best human practice prize;</tr></td></div> <br />
<br />
<div> <br />
<tr><td>1) Firstly, we aimed in our project, obtain an anti gram negative surface with using B. subtilis and E.coli bacteria. E. coli is killing by B. subtilis with B. subtilis’s own new protein LALF. But B. subtilis is a gram positive and this surface can’t protect us from some pathogenic effects of B.Subtilis’s spores. For protecting from B. subtilis we used the sporocide method as a non-used B.subtilis procedure in iGEM useful for further B.subtilis synthetic biology projects. Fenton Reagent in Sporocide methode is making our project more safety and useful as wanted in Human practice safety issue approaching way of our project. </tr></td></div> <br />
<br />
<div><tr><td>2) Secondly we have informed people about our project, synthetic biology and iGEM competition, and draw their attention to genetics.</td></tr></div><br />
<br />
<div><tr><td>- We prepared a board game named “The Canvas Town” to inform people from any distinct education level about features of our project, laboratory equipments and interesting Biobrick parts of iGEM competıtion. We have played several times with lots of people some of them are a group of gifted children and the Turkey’s other iGEM teams and it was very funny and informative. </tr></td></div><br />
<br />
<div><tr><td>-Our one of the most important goals is to introduce iGEM and Synthetic Biology to the students in Turkey. But we did not want to limit ourselves by choosing only same age groups and we asked to several ages of people “what do you want from your bacteria to do for you?” With this question we went to a kindergarten and we gave very interesting answers. The children were very interested about genetics, synthetic biology and microorganisms. Also we asked the same question to students of our university and the ideas were very funny and useful for synthetic biology. With these activities we drew their attention to genetics, synthetic biology and iGEM. </td></tr></div><br />
<br />
<div><tr><td>-Second International Medical Student Conference in Ankara had performed on April 2011 and it’s organized by University of Fatih Medical Faculty. In the conference, synthetic biology and iGEM competition related presentation was performed to inform medical school students coming from different medical schools in Turkey and also from out of Turkey ie. Japan.</td></tr></div><br />
<br />
<div><tr><td>3) Thirdly we have showed people that the benefits of synthetic biology and microorganisms </div><br />
<br />
<div>-We strove for the innovation and presentation of synthetic biology to people. As a medical school, our hospital has its own quarterly magazine, “Yasama Sanati” (in English: The Art of Living). We decided that we could mention about iGEM and synthetic biology; thus we wrote an article that told the bacteria is not too much harmful living creature to humans as it is seen nowadays. In fact, we mentioned that bacteria would become very handy, helpful and intelligent “machines” in appropriate conditions. We listed the former experiments and parts that took place in iGEM competition and gave some examples about what to do next. With this article lots of people informed and their some negative sentiments about the microorganisms and synthetic biology would be changed.</td></tr></div><br />
<br />
<div><tr><td>4) We have prepared a journal which is named “Canvas Times” and it gives you the actual news of our lab and team. Also you can learn about our project and read our columnist and you can travel our peace and quiet town the Canvas Town. “Canvas Times” a new journal of young scientists; it gives you the actual news of our lab and team. <br />
During our lab working, we could find some time to fun and talk with people about our project, synthetic biology and iGEM... Our all of activities and human practices are existed on our journal such as “iGEM Picnic” and “International Medical Students Conference. Therewithal you will find some information of our university and faculty. You can find it all of stores.</div><br />
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Team:Fatih Turkey/Human Practice
2011-09-22T01:30:47Z
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<h2 style="font-family: Verdana, Arial, SunSans-Regular, sans-serif;">Human Practice</h2><br />
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<td><h2><a href="https://2011.igem.org/Team:Fatih_Turkey/Sporocide">Sporocide</a></h2></td><br />
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<tr><td>To remove infections B.subtilis spores from our Anti-LPS factor containing biofilm paste, fenton reagent is used before use against E.coli.</td></tr><br />
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<tr><td>During funny summer in our project executions, we have also met hundreds of people from different ages in order to tell our project in iGEM competition and benefits of synthetic biology.</td></tr><br />
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<tr><td>We have designed monopoly like game name "The Canvas Town" to inform people from any distinct education level about features of our project, laboratory equipments and interesting Biobrick of iGEM competition.</td></tr><br />
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<tr><td>a new journal of young scientists; it gives you the actual news of our lab and team</td></tr><br />
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<br />
<h1>Outline and Detailed Human Practice</h1></div><br />
<br />
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<tr><td>During our Human Practice studies, we have tried to tell our project, its usage areas, synthetic better life not only to our medical student friends coming from different schools through Turkey but also citizens, childrens, highschool students with the help of `Canvas Town` our novel board game, `iGEM for 7 to 77` our weekly open-lab to the public meetings and our `Canvas Times` great summer magazine containing all our human practice activities. <br />
To be able to reach the best human practice prize;</tr></td></div> <br />
<br />
<div> <br />
<tr><td>1) Firstly, we aimed in our project, obtain an anti gram negative surface with using B. subtilis and E.coli bacteria. E. coli is killing by B. subtilis with B. subtilis’s own new protein LALF. But B. subtilis is a gram positive and this surface can’t protect us from some pathogenic effects of B.Subtilis’s spores. For protecting from B. subtilis we used the sporocide method as a non-used B.subtilis procedure in iGEM useful for further B.subtilis synthetic biology projects. Fenton Reagent in Sporocide methode is making our project more safety and useful as wanted in Human practice safety issue approaching way of our project. </tr></td></div> <br />
<br />
<div><tr><td>2) Secondly we have informed people about our project, synthetic biology and iGEM competition, and draw their attention to genetics.</td></tr></div><br />
<br />
<div><tr><td>- We prepared a board game named “The Canvas Town” to inform people from any distinct education level about features of our project, laboratory equipments and interesting Biobrick parts of iGEM competıtion. We have played several times with lots of people some of them are a group of gifted children and the Turkey’s other iGEM teams and it was very funny and informative. </tr></td></div><br />
<br />
<div><tr><td>-Our one of the most important goals is to introduce iGEM and Synthetic Biology to the students in Turkey. But we did not want to limit ourselves by choosing only same age groups and we asked to several ages of people “what do you want from your bacteria to do for you?” With this question we went to a kindergarten and we gave very interesting answers. The children were very interested about genetics, synthetic biology and microorganisms. Also we asked the same question to students of our university and the ideas were very funny and useful for synthetic biology. With these activities we drew their attention to genetics, synthetic biology and iGEM. </td></tr></div><br />
<br />
<div><tr><td>-Second International Medical Student Conference in Ankara had performed on April 2011 and it’s organized by University of Fatih Medical Faculty. In the conference, synthetic biology and iGEM competition related presentation was performed to inform medical school students coming from different medical schools in Turkey and also from out of Turkey ie. Japan.</td></tr></div><br />
<br />
<div><tr><td>3) Thirdly we have showed people that the benefits of synthetic biology and microorganisms </div><br />
<br />
<div>-We strove for the innovation and presentation of synthetic biology to people. As a medical school, our hospital has its own quarterly magazine, “Yasama Sanati” (in English: The Art of Living). We decided that we could mention about iGEM and synthetic biology; thus we wrote an article that told the bacteria is not too much harmful living creature to humans as it is seen nowadays. In fact, we mentioned that bacteria would become very handy, helpful and intelligent “machines” in appropriate conditions. We listed the former experiments and parts that took place in iGEM competition and gave some examples about what to do next. With this article lots of people informed and their some negative sentiments about the microorganisms and synthetic biology would be changed.</td></tr></div><br />
<br />
<div><tr><td>4) We have prepared a journal which is named “Canvas Times” and it gives you the actual news of our lab and team. Also you can learn about our project and read our columnist and you can travel our peace and quiet town the Canvas Town. “Canvas Times” a new journal of young scientists; it gives you the actual news of our lab and team. <br />
During our lab working, we could find some time to fun and talk with people about our project, synthetic biology and iGEM... Our all of activities and human practices are existed on our journal such as “iGEM Picnic” and “International Medical Students Conference. Therewithal you will find some information of our university and faculty. You can find it all of stores.</div><br />
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http://2011.igem.org/Team:Fatih_Turkey/Human_Practice
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2011-09-22T01:29:43Z
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<h2 style="font-family: Verdana, Arial, SunSans-Regular, sans-serif;">Human Practice</h2><br />
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<tr><td>To remove infections B.subtilis spores from our Anti-LPS factor containing biofilm paste, fenton reagent is used before use against E.coli.</td></tr><br />
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<tr><td>During funny summer in our project executions, we have also met hundreds of people from different ages in order to tell our project in iGEM competition and benefits of synthetic biology.</td></tr><br />
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<tr><td>We have designed monopoly like game name "The Canvas Town" to inform people from any distinct education level about features of our project, laboratory equipments and interesting Biobrick of iGEM competition.</td></tr><br />
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<tr><td>a new journal of young scientists; it gives you the actual news of our lab and team</td></tr><br />
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<h1>Outline and Detailed Human Practice</h1></div><br />
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<tr><td>During our Human Practice studies, we have tried to tell our project, its usage areas, synthetic better life not only to our medical student friends coming from different schools through Turkey but also citizens, childrens, highschool students with the help of `Canvas Town` our novel board game, `iGEM for 7 to 77` our weekly open-lab to the public meetings and our `Canvas Times` great summer magazine containing all our human practice activities. <br />
To be able to reach the best human practice prize;</tr></td></div> <br />
<br />
<div> <br />
<tr><td>1) Firstly, we aimed in our project, obtain an anti gram negative surface with using B. subtilis and E.coli bacteria. E. coli is killing by B. subtilis with B. subtilis’s own new protein LALF. But B. subtilis is a gram positive and this surface can’t protect us from some pathogenic effects of B.Subtilis’s spores. For protecting from B. subtilis we used the sporocide method as a non-used B.subtilis procedure in iGEM useful for further B.subtilis synthetic biology projects. Fenton Reagent in Sporocide methode is making our project more safety and useful as wanted in Human practice safety issue approaching way of our project. </tr></td></div> <br />
<br />
<div><tr><td>2) Secondly we have informed people about our project, synthetic biology and iGEM competition, and draw their attention to genetics.</td></tr></div><br />
<br />
<div><tr><td>- We prepared a board game named “The Canvas Town” to inform people from any distinct education level about features of our project, laboratory equipments and interesting Biobrick parts of iGEM competıtion. We have played several times with lots of people some of them are a group of gifted children and the Turkey’s other iGEM teams and it was very funny and informative. </tr></td></div><br />
<br />
<div><tr><td>-Our one of the most important goals is to introduce iGEM and Synthetic Biology to the students in Turkey. But we did not want to limit ourselves by choosing only same age groups and we asked to several ages of people “what do you want from your bacteria to do for you?” With this question we went to a kindergarten and we gave very interesting answers. The children were very interested about genetics, synthetic biology and microorganisms. Also we asked the same question to students of our university and the ideas were very funny and useful for synthetic biology. With these activities we drew their attention to genetics, synthetic biology and iGEM. </td></tr></div><br />
<br />
<div><tr><td>-Second International Medical Student Conference in Ankara had performed on April 2011 and it’s organized by University of Fatih Medical Faculty. In the conference, synthetic biology and iGEM competition related presentation was performed to inform medical school students coming from different medical schools in Turkey and also from out of Turkey ie. Japan.</td></tr></div><br />
<br />
<div><tr><td>3) Thirdly we have showed people that the benefits of synthetic biology and microorganisms </div><br />
<br />
<div>-We strove for the innovation and presentation of synthetic biology to people. As a medical school, our hospital has its own quarterly magazine, “Yasama Sanati” (in English: The Art of Living). We decided that we could mention about iGEM and synthetic biology; thus we wrote an article that told the bacteria is not too much harmful living creature to humans as it is seen nowadays. In fact, we mentioned that bacteria would become very handy, helpful and intelligent “machines” in appropriate conditions. We listed the former experiments and parts that took place in iGEM competition and gave some examples about what to do next. With this article lots of people informed and their some negative sentiments about the microorganisms and synthetic biology would be changed.</td></tr></div><br />
<br />
<div><tr><td>4) We have prepared a journal which is named “Canvas Times” and it gives you the actual news of our lab and team. Also you can learn about our project and read our columnist and you can travel our peace and quiet town the Canvas Town. “Canvas Times” a new journal of young scientists; it gives you the actual news of our lab and team. <br />
During our lab working, we could find some time to fun and talk with people about our project, synthetic biology and iGEM... Our all of activities and human practices are existed on our journal such as “iGEM Picnic” and “International Medical Students Conference. Therewithal you will find some information of our university and faculty. You can find it all of stores.</div><br />
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http://2011.igem.org/Team:Fatih_Turkey/Human_Practice
Team:Fatih Turkey/Human Practice
2011-09-22T01:27:54Z
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<h2 style="font-family: Verdana, Arial, SunSans-Regular, sans-serif;">Human Practice</h2><br />
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<tr><td>To remove infections B.subtilis spores from our Anti-LPS factor containing biofilm paste, fenton reagent is used before use against E.coli.</td></tr><br />
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<td><h2><a href="https://2011.igem.org/Team:Fatih_Turkey/iGEM_for_7_to_77">iGEM for 7 to 77</a></h2></td><br />
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<tr><td>During funny summer in our project executions, we have also met hundreds of people from different ages in order to tell our project in iGEM competition and benefits of synthetic biology.</td></tr><br />
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<tr><td>We have designed monopoly like game name "The Canvas Town" to inform people from any distinct education level about features of our project, laboratory equipments and interesting Biobrick of iGEM competition.</td></tr><br />
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<tr><td>a new journal of young scientists; it gives you the actual news of our lab and team</td></tr><br />
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<tr><td>During our Human Practice studies, we have tried to tell our project, its usage areas, synthetic better life not only to our medical student friends coming from different schools through Turkey but also citizens, childrens, highschool students with the help of `Canvas Town` our novel board game, `iGEM for 7 to 77` our weekly open-lab to the public meetings and our `Canvas Times` great summer magazine containing all our human practice activities. <br />
To be able to reach the best human practice prize;</tr></td></div> <br />
<br />
<div> <br />
<tr><td>1) Firstly, we aimed in our project, obtain an anti gram negative surface with using B. subtilis and E.coli bacteria. E. coli is killing by B. subtilis with B. subtilis’s own new protein LALF. But B. subtilis is a gram positive and this surface can’t protect us from some pathogenic effects of B.Subtilis’s spores. For protecting from B. subtilis we used the sporocide method as a non-used B.subtilis procedure in iGEM useful for further B.subtilis synthetic biology projects. Fenton Reagent in Sporocide methode is making our project more safety and useful as wanted in Human practice safety issue approaching way of our project. </tr></td></div> <br />
<br />
<div><tr><td>2) Secondly we have informed people about our project, synthetic biology and iGEM competition, and draw their attention to genetics.</td></tr></div><br />
<br />
<div><tr><td>- We prepared a board game named “The Canvas Town” to inform people from any distinct education level about features of our project, laboratory equipments and interesting Biobrick parts of iGEM competıtion. We have played several times with lots of people some of them are a group of gifted children and the Turkey’s other iGEM teams and it was very funny and informative. </tr></td></div><br />
<br />
<div><tr><td>-Our one of the most important goals is to introduce iGEM and Synthetic Biology to the students in Turkey. But we did not want to limit ourselves by choosing only same age groups and we asked to several ages of people “what do you want from your bacteria to do for you?” With this question we went to a kindergarten and we gave very interesting answers. The children were very interested about genetics, synthetic biology and microorganisms. Also we asked the same question to students of our university and the ideas were very funny and useful for synthetic biology. With these activities we drew their attention to genetics, synthetic biology and iGEM. </td></tr></div><br />
<br />
<div><tr><td>-Second International Medical Student Conference in Ankara had performed on April 2011 and it’s organized by University of Fatih Medical Faculty. In the conference, synthetic biology and iGEM competition related presentation was performed to inform medical school students coming from different medical schools in Turkey and also from out of Turkey ie. Japan.</td></tr></div><br />
<br />
<div><tr><td>3) Thirdly we have showed people that the benefits of synthetic biology and microorganisms </div><br />
<br />
<div>-We strove for the innovation and presentation of synthetic biology to people. As a medical school, our hospital has its own quarterly magazine, “Yasama Sanati” (in English: The Art of Living). We decided that we could mention about iGEM and synthetic biology; thus we wrote an article that told the bacteria is not too much harmful living creature to humans as it is seen nowadays. In fact, we mentioned that bacteria would become very handy, helpful and intelligent “machines” in appropriate conditions. We listed the former experiments and parts that took place in iGEM competition and gave some examples about what to do next. With this article lots of people informed and their some negative sentiments about the microorganisms and synthetic biology would be changed.</td></tr></div><br />
<br />
<div><tr><td>4) We have prepared a journal which is named “Canvas Times” and it gives you the actual news of our lab and team. Also you can learn about our project and read our columnist and you can travel our peace and quiet town the Canvas Town. “Canvas Times” a new journal of young scientists; it gives you the actual news of our lab and team. <br />
During our lab working, we could find some time to fun and talk with people about our project, synthetic biology and iGEM... Our all of activities and human practices are existed on our journal such as “iGEM Picnic” and “International Medical Students Conference. Therewithal you will find some information of our university and faculty. You can find it all of stores.</div><br />
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Metugen
http://2011.igem.org/Team:Fatih_Turkey/Human_Practice
Team:Fatih Turkey/Human Practice
2011-09-22T01:26:38Z
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<h2 style="font-family: Verdana, Arial, SunSans-Regular, sans-serif;">Human Practice</h2><br />
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<td><h2><a href="https://2011.igem.org/Team:Fatih_Turkey/Sporocide">Sporocide</a></h2></td><br />
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<tr><td>To remove infections B.subtilis spores from our Anti-LPS factor containing biofilm paste, fenton reagent is used before use against E.coli.</td></tr><br />
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<td><h2><a href="https://2011.igem.org/Team:Fatih_Turkey/iGEM_for_7_to_77">iGEM for 7 to 77</a></h2></td><br />
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<tr><td>During funny summer in our project executions, we have also met hundreds of people from different ages in order to tell our project in iGEM competition and benefits of synthetic biology.</td></tr><br />
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<tr><td>We have designed monopoly like game name "The Canvas Town" to inform people from any distinct education level about features of our project, laboratory equipments and interesting Biobrick of iGEM competition.</td></tr><br />
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<tr><td>a new journal of young scientists; it gives you the actual news of our lab and team</td></tr><br />
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<tr><td>During our Human Practice studies, we have tried to tell our project, its usage areas, synthetic better life not only to our medical student friends coming from different schools through Turkey but also citizens, childrens, highschool students with the help of `Canvas Town` our novel board game, `iGEM for 7 to 77` our weekly open-lab to the public meetings and our `Canvas Times` great summer magazine containing all our human practice activities. <br />
To be able to reach the best human practice prize;</tr></td></div> <br />
<div> <br />
<tr><td>1) Firstly, we aimed in our project, obtain an anti gram negative surface with using B. subtilis and E.coli bacteria. E. coli is killing by B. subtilis with B. subtilis’s own new protein LALF. But B. subtilis is a gram positive and this surface can’t protect us from some pathogenic effects of B.Subtilis’s spores. For protecting from B. subtilis we used the sporocide method as a non-used B.subtilis procedure in iGEM useful for further B.subtilis synthetic biology projects. Fenton Reagent in Sporocide methode is making our project more safety and useful as wanted in Human practice safety issue approaching way of our project. </tr></td></div> <br />
<br />
<tr><td>2) Secondly we have informed people about our project, synthetic biology and iGEM competition, and draw their attention to genetics.</td></tr><br />
<br />
<tr><td>- We prepared a board game named “The Canvas Town” to inform people from any distinct education level about features of our project, laboratory equipments and interesting Biobrick parts of iGEM competıtion. We have played several times with lots of people some of them are a group of gifted children and the Turkey’s other iGEM teams and it was very funny and informative. </tr></td><br />
<br />
<tr><td>-Our one of the most important goals is to introduce iGEM and Synthetic Biology to the students in Turkey. But we did not want to limit ourselves by choosing only same age groups and we asked to several ages of people “what do you want from your bacteria to do for you?” With this question we went to a kindergarten and we gave very interesting answers. The children were very interested about genetics, synthetic biology and microorganisms. Also we asked the same question to students of our university and the ideas were very funny and useful for synthetic biology. With these activities we drew their attention to genetics, synthetic biology and iGEM. </td></tr><br />
<br />
<tr><td>-Second International Medical Student Conference in Ankara had performed on April 2011 and it’s organized by University of Fatih Medical Faculty. In the conference, synthetic biology and iGEM competition related presentation was performed to inform medical school students coming from different medical schools in Turkey and also from out of Turkey ie. Japan.</td></tr><br />
<br />
<tr><td>3) Thirdly we have showed people that the benefits of synthetic biology and microorganisms <br />
-We strove for the innovation and presentation of synthetic biology to people. As a medical school, our hospital has its own quarterly magazine, “Yasama Sanati” (in English: The Art of Living). We decided that we could mention about iGEM and synthetic biology; thus we wrote an article that told the bacteria is not too much harmful living creature to humans as it is seen nowadays. In fact, we mentioned that bacteria would become very handy, helpful and intelligent “machines” in appropriate conditions. We listed the former experiments and parts that took place in iGEM competition and gave some examples about what to do next. With this article lots of people informed and their some negative sentiments about the microorganisms and synthetic biology would be changed.</td></tr><br />
<br />
<tr><td>4) We have prepared a journal which is named “Canvas Times” and it gives you the actual news of our lab and team. Also you can learn about our project and read our columnist and you can travel our peace and quiet town the Canvas Town. “Canvas Times” a new journal of young scientists; it gives you the actual news of our lab and team. <br />
During our lab working, we could find some time to fun and talk with people about our project, synthetic biology and iGEM... Our all of activities and human practices are existed on our journal such as “iGEM Picnic” and “International Medical Students Conference. Therewithal you will find some information of our university and faculty. You can find it all of stores.<br />
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Metugen
http://2011.igem.org/Team:Fatih_Turkey/Human_Practice
Team:Fatih Turkey/Human Practice
2011-09-22T01:25:58Z
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<h2 style="font-family: Verdana, Arial, SunSans-Regular, sans-serif;">Human Practice</h2><br />
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<td><h2><a href="https://2011.igem.org/Team:Fatih_Turkey/Sporocide">Sporocide</a></h2></td><br />
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<tr><td>To remove infections B.subtilis spores from our Anti-LPS factor containing biofilm paste, fenton reagent is used before use against E.coli.</td></tr><br />
</td><br />
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<td><h2><a href="https://2011.igem.org/Team:Fatih_Turkey/iGEM_for_7_to_77">iGEM for 7 to 77</a></h2></td><br />
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<tr><td>During funny summer in our project executions, we have also met hundreds of people from different ages in order to tell our project in iGEM competition and benefits of synthetic biology.</td></tr><br />
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<td><h2><a href="https://2011.igem.org/Team:Fatih_Turkey/game">Game</a></h2></td><br />
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<tr><td>We have designed monopoly like game name "The Canvas Town" to inform people from any distinct education level about features of our project, laboratory equipments and interesting Biobrick of iGEM competition.</td></tr><br />
</td><br />
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<td><h2><a href="https://2011.igem.org/Team:Fatih_Turkey/canvas_times">Canvas Times</a></h2></td><br />
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<tr><td>a new journal of young scientists; it gives you the actual news of our lab and team</td></tr><br />
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<tr><td>'''During our Human Practice studies,''' we have tried to tell our project, its usage areas, synthetic better life not only to our medical student friends coming from different schools through Turkey but also citizens, childrens, highschool students with the help of `Canvas Town` our novel board game, `iGEM for 7 to 77` our weekly open-lab to the public meetings and our `Canvas Times` great summer magazine containing all our human practice activities. <br />
To be able to reach the best human practice prize;</tr></td><br />
<br />
<br />
<tr><td>1) Firstly, we aimed in our project, obtain an anti gram negative surface with using B. subtilis and E.coli bacteria. E. coli is killing by B. subtilis with B. subtilis’s own new protein LALF. But B. subtilis is a gram positive and this surface can’t protect us from some pathogenic effects of B.Subtilis’s spores. For protecting from B. subtilis we used the sporocide method as a non-used B.subtilis procedure in iGEM useful for further B.subtilis synthetic biology projects. Fenton Reagent in Sporocide methode is making our project more safety and useful as wanted in Human practice safety issue approaching way of our project. </tr></td><br />
<br />
<tr><td>2) Secondly we have informed people about our project, synthetic biology and iGEM competition, and draw their attention to genetics.</td></tr><br />
<br />
<tr><td>- We prepared a board game named “The Canvas Town” to inform people from any distinct education level about features of our project, laboratory equipments and interesting Biobrick parts of iGEM competıtion. We have played several times with lots of people some of them are a group of gifted children and the Turkey’s other iGEM teams and it was very funny and informative. </tr></td><br />
<br />
<tr><td>-Our one of the most important goals is to introduce iGEM and Synthetic Biology to the students in Turkey. But we did not want to limit ourselves by choosing only same age groups and we asked to several ages of people “what do you want from your bacteria to do for you?” With this question we went to a kindergarten and we gave very interesting answers. The children were very interested about genetics, synthetic biology and microorganisms. Also we asked the same question to students of our university and the ideas were very funny and useful for synthetic biology. With these activities we drew their attention to genetics, synthetic biology and iGEM. </td></tr><br />
<br />
<tr><td>-Second International Medical Student Conference in Ankara had performed on April 2011 and it’s organized by University of Fatih Medical Faculty. In the conference, synthetic biology and iGEM competition related presentation was performed to inform medical school students coming from different medical schools in Turkey and also from out of Turkey ie. Japan.</td></tr><br />
<br />
<tr><td>3) Thirdly we have showed people that the benefits of synthetic biology and microorganisms <br />
-We strove for the innovation and presentation of synthetic biology to people. As a medical school, our hospital has its own quarterly magazine, “Yasama Sanati” (in English: The Art of Living). We decided that we could mention about iGEM and synthetic biology; thus we wrote an article that told the bacteria is not too much harmful living creature to humans as it is seen nowadays. In fact, we mentioned that bacteria would become very handy, helpful and intelligent “machines” in appropriate conditions. We listed the former experiments and parts that took place in iGEM competition and gave some examples about what to do next. With this article lots of people informed and their some negative sentiments about the microorganisms and synthetic biology would be changed.</td></tr><br />
<br />
<tr><td>4) We have prepared a journal which is named “Canvas Times” and it gives you the actual news of our lab and team. Also you can learn about our project and read our columnist and you can travel our peace and quiet town the Canvas Town. “Canvas Times” a new journal of young scientists; it gives you the actual news of our lab and team. <br />
During our lab working, we could find some time to fun and talk with people about our project, synthetic biology and iGEM... Our all of activities and human practices are existed on our journal such as “iGEM Picnic” and “International Medical Students Conference. Therewithal you will find some information of our university and faculty. You can find it all of stores.<br />
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Metugen
http://2011.igem.org/Team:Fatih_Turkey/Human_Practice
Team:Fatih Turkey/Human Practice
2011-09-22T01:25:31Z
<p>Metugen: </p>
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<tr><td>To remove infections B.subtilis spores from our Anti-LPS factor containing biofilm paste, fenton reagent is used before use against E.coli.</td></tr><br />
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<tr><td>During funny summer in our project executions, we have also met hundreds of people from different ages in order to tell our project in iGEM competition and benefits of synthetic biology.</td></tr><br />
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<td><h2><a href="https://2011.igem.org/Team:Fatih_Turkey/game">Game</a></h2></td><br />
</tr><br />
<tr><td>We have designed monopoly like game name "The Canvas Town" to inform people from any distinct education level about features of our project, laboratory equipments and interesting Biobrick of iGEM competition.</td></tr><br />
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<a href="https://2011.igem.org/Team:Fatih_Turkey/canvas_times"><img src="https://static.igem.org/mediawiki/2011/0/05/Canvasicon.jpg" width="160" /></a><br />
</td><br />
<td width="600" style="vertical-align:top;"><br />
<table><br />
<tr><br />
<td><h2><a href="https://2011.igem.org/Team:Fatih_Turkey/canvas_times">Canvas Times</a></h2></td><br />
</tr><br />
<tr><td>a new journal of young scientists; it gives you the actual news of our lab and team</td></tr><br />
</td><br />
</tr><br />
</table><br />
</td><br />
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</table> <br />
<br />
<tr><td>During our Human Practice studies, we have tried to tell our project, its usage areas, synthetic better life not only to our medical student friends coming from different schools through Turkey but also citizens, childrens, highschool students with the help of `Canvas Town` our novel board game, `iGEM for 7 to 77` our weekly open-lab to the public meetings and our `Canvas Times` great summer magazine containing all our human practice activities. <br />
To be able to reach the best human practice prize;</tr></td><br />
<br />
<br />
<tr><td>1) Firstly, we aimed in our project, obtain an anti gram negative surface with using B. subtilis and E.coli bacteria. E. coli is killing by B. subtilis with B. subtilis’s own new protein LALF. But B. subtilis is a gram positive and this surface can’t protect us from some pathogenic effects of B.Subtilis’s spores. For protecting from B. subtilis we used the sporocide method as a non-used B.subtilis procedure in iGEM useful for further B.subtilis synthetic biology projects. Fenton Reagent in Sporocide methode is making our project more safety and useful as wanted in Human practice safety issue approaching way of our project. </tr></td><br />
<br />
<tr><td>2) Secondly we have informed people about our project, synthetic biology and iGEM competition, and draw their attention to genetics.</td></tr><br />
<br />
<tr><td>- We prepared a board game named “The Canvas Town” to inform people from any distinct education level about features of our project, laboratory equipments and interesting Biobrick parts of iGEM competıtion. We have played several times with lots of people some of them are a group of gifted children and the Turkey’s other iGEM teams and it was very funny and informative. </tr></td><br />
<br />
<tr><td>-Our one of the most important goals is to introduce iGEM and Synthetic Biology to the students in Turkey. But we did not want to limit ourselves by choosing only same age groups and we asked to several ages of people “what do you want from your bacteria to do for you?” With this question we went to a kindergarten and we gave very interesting answers. The children were very interested about genetics, synthetic biology and microorganisms. Also we asked the same question to students of our university and the ideas were very funny and useful for synthetic biology. With these activities we drew their attention to genetics, synthetic biology and iGEM. </td></tr><br />
<br />
<tr><td>-Second International Medical Student Conference in Ankara had performed on April 2011 and it’s organized by University of Fatih Medical Faculty. In the conference, synthetic biology and iGEM competition related presentation was performed to inform medical school students coming from different medical schools in Turkey and also from out of Turkey ie. Japan.</td></tr><br />
<br />
<tr><td>3) Thirdly we have showed people that the benefits of synthetic biology and microorganisms <br />
-We strove for the innovation and presentation of synthetic biology to people. As a medical school, our hospital has its own quarterly magazine, “Yasama Sanati” (in English: The Art of Living). We decided that we could mention about iGEM and synthetic biology; thus we wrote an article that told the bacteria is not too much harmful living creature to humans as it is seen nowadays. In fact, we mentioned that bacteria would become very handy, helpful and intelligent “machines” in appropriate conditions. We listed the former experiments and parts that took place in iGEM competition and gave some examples about what to do next. With this article lots of people informed and their some negative sentiments about the microorganisms and synthetic biology would be changed.</td></tr><br />
<br />
<tr><td>4) We have prepared a journal which is named “Canvas Times” and it gives you the actual news of our lab and team. Also you can learn about our project and read our columnist and you can travel our peace and quiet town the Canvas Town. “Canvas Times” a new journal of young scientists; it gives you the actual news of our lab and team. <br />
During our lab working, we could find some time to fun and talk with people about our project, synthetic biology and iGEM... Our all of activities and human practices are existed on our journal such as “iGEM Picnic” and “International Medical Students Conference. Therewithal you will find some information of our university and faculty. You can find it all of stores.<br />
</td></tr><br />
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http://2011.igem.org/Team:Fatih_Turkey/Human_Practice
Team:Fatih Turkey/Human Practice
2011-09-22T01:17:07Z
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<h2 style="font-family: Verdana, Arial, SunSans-Regular, sans-serif;">Human Practice</h2><br />
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<td><h2><a href="https://2011.igem.org/Team:Fatih_Turkey/Sporocide">Sporocide</a></h2></td><br />
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<tr><td>To remove infections B.subtilis spores from our Anti-LPS factor containing biofilm paste, fenton reagent is used before use against E.coli.</td></tr><br />
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<td><h2><a href="https://2011.igem.org/Team:Fatih_Turkey/iGEM_for_7_to_77">iGEM for 7 to 77</a></h2></td><br />
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<tr><td>During funny summer in our project executions, we have also met hundreds of people from different ages in order to tell our project in iGEM competition and benefits of synthetic biology.</td></tr><br />
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<tr><td>We have designed monopoly like game name "The Canvas Town" to inform people from any distinct education level about features of our project, laboratory equipments and interesting Biobrick of iGEM competition.</td></tr><br />
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<tr><td>a new journal of young scientists; it gives you the actual news of our lab and team</td></tr><br />
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<tr><td>During our Human Practice studies, we have tried to tell our project, its usage areas, synthetic better life not only to our medical student friends coming from different schools through Turkey but also citizens, childrens, highschool students with the help of `Canvas Town` our novel board game, `iGEM for 7 to 77` our weekly open-lab to the public meetings and our `Canvas Times` great summer magazine containing all our human practice activities. <br />
To be able to reach the best human practice prize;</tr></td><br />
<tr><td>1) Firstly, we aimed in our project, obtain an anti gram negative surface with using B. subtilis and E.coli bacteria. E. coli is killing by B. subtilis with B. subtilis’s own new protein LALF. But B. subtilis is a gram positive and this surface can’t protect us from some pathogenic effects of B.Subtilis’s spores. For protecting from B. subtilis we used the sporocide method as a non-used B.subtilis procedure in iGEM useful for further B.subtilis synthetic biology projects. Fenton Reagent in Sporocide methode is making our project more safety and useful as wanted in Human practice safety issue approaching way of our project. <br />
<br />
2) Secondly we have informed people about our project, synthetic biology and iGEM competition, and draw their attention to genetics.<br />
- We prepared a board game named “The Canvas Town” to inform people from any distinct education level about features of our project, laboratory equipments and interesting Biobrick parts of iGEM competıtion. We have played several times with lots of people some of them are a group of gifted children and the Turkey’s other iGEM teams and it was very funny and informative. <br />
<br />
-Our one of the most important goals is to introduce iGEM and Synthetic Biology to the students in Turkey. But we did not want to limit ourselves by choosing only same age groups and we asked to several ages of people “what do you want from your bacteria to do for you?” With this question we went to a kindergarten and we gave very interesting answers. The children were very interested about genetics, synthetic biology and microorganisms. Also we asked the same question to students of our university and the ideas were very funny and useful for synthetic biology. With these activities we drew their attention to genetics, synthetic biology and iGEM. <br />
<br />
-Second International Medical Student Conference in Ankara had performed on April 2011 and it’s organized by University of Fatih Medical Faculty. In the conference, synthetic biology and iGEM competition related presentation was performed to inform medical school students coming from different medical schools in Turkey and also from out of Turkey ie. Japan.<br />
3) Thirdly we have showed people that the benefits of synthetic biology and microorganisms <br />
-We strove for the innovation and presentation of synthetic biology to people. As a medical school, our hospital has its own quarterly magazine, “Yasama Sanati” (in English: The Art of Living). We decided that we could mention about iGEM and synthetic biology; thus we wrote an article that told the bacteria is not too much harmful living creature to humans as it is seen nowadays. In fact, we mentioned that bacteria would become very handy, helpful and intelligent “machines” in appropriate conditions. We listed the former experiments and parts that took place in iGEM competition and gave some examples about what to do next. With this article lots of people informed and their some negative sentiments about the microorganisms and synthetic biology would be changed.<br />
<br />
4) We have prepared a journal which is named “Canvas Times” and it gives you the actual news of our lab and team. Also you can learn about our project and read our columnist and you can travel our peace and quiet town the Canvas Town. “Canvas Times” a new journal of young scientists; it gives you the actual news of our lab and team. <br />
During our lab working, we could find some time to fun and talk with people about our project, synthetic biology and iGEM... Our all of activities and human practices are existed on our journal such as “iGEM Picnic” and “International Medical Students Conference. Therewithal you will find some information of our university and faculty. You can find it all of stores.<br />
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2011-09-22T01:16:21Z
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<h2 style="font-family: Verdana, Arial, SunSans-Regular, sans-serif;">Human Practice</h2><br />
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<td><h2><a href="https://2011.igem.org/Team:Fatih_Turkey/Sporocide">Sporocide</a></h2></td><br />
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<tr><td>To remove infections B.subtilis spores from our Anti-LPS factor containing biofilm paste, fenton reagent is used before use against E.coli.</td></tr><br />
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<tr><td>During funny summer in our project executions, we have also met hundreds of people from different ages in order to tell our project in iGEM competition and benefits of synthetic biology.</td></tr><br />
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<tr><td>We have designed monopoly like game name "The Canvas Town" to inform people from any distinct education level about features of our project, laboratory equipments and interesting Biobrick of iGEM competition.</td></tr><br />
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<tr><td>a new journal of young scientists; it gives you the actual news of our lab and team</td></tr><br />
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<tr><td>During our Human Practice studies, we have tried to tell our project, its usage areas, synthetic better life not only to our medical student friends coming from different schools through Turkey but also citizens, childrens, highschool students with the help of `Canvas Town` our novel board game, `iGEM for 7 to 77` our weekly open-lab to the public meetings and our `Canvas Times` great summer magazine containing all our human practice activities. <br />
To be able to reach the best human practice prize;<br />
1) Firstly, we aimed in our project, obtain an anti gram negative surface with using B. subtilis and E.coli bacteria. E. coli is killing by B. subtilis with B. subtilis’s own new protein LALF. But B. subtilis is a gram positive and this surface can’t protect us from some pathogenic effects of B.Subtilis’s spores. For protecting from B. subtilis we used the sporocide method as a non-used B.subtilis procedure in iGEM useful for further B.subtilis synthetic biology projects. Fenton Reagent in Sporocide methode is making our project more safety and useful as wanted in Human practice safety issue approaching way of our project. <br />
<br />
2) Secondly we have informed people about our project, synthetic biology and iGEM competition, and draw their attention to genetics.<br />
- We prepared a board game named “The Canvas Town” to inform people from any distinct education level about features of our project, laboratory equipments and interesting Biobrick parts of iGEM competıtion. We have played several times with lots of people some of them are a group of gifted children and the Turkey’s other iGEM teams and it was very funny and informative. <br />
<br />
-Our one of the most important goals is to introduce iGEM and Synthetic Biology to the students in Turkey. But we did not want to limit ourselves by choosing only same age groups and we asked to several ages of people “what do you want from your bacteria to do for you?” With this question we went to a kindergarten and we gave very interesting answers. The children were very interested about genetics, synthetic biology and microorganisms. Also we asked the same question to students of our university and the ideas were very funny and useful for synthetic biology. With these activities we drew their attention to genetics, synthetic biology and iGEM. <br />
<br />
-Second International Medical Student Conference in Ankara had performed on April 2011 and it’s organized by University of Fatih Medical Faculty. In the conference, synthetic biology and iGEM competition related presentation was performed to inform medical school students coming from different medical schools in Turkey and also from out of Turkey ie. Japan.<br />
3) Thirdly we have showed people that the benefits of synthetic biology and microorganisms <br />
-We strove for the innovation and presentation of synthetic biology to people. As a medical school, our hospital has its own quarterly magazine, “Yasama Sanati” (in English: The Art of Living). We decided that we could mention about iGEM and synthetic biology; thus we wrote an article that told the bacteria is not too much harmful living creature to humans as it is seen nowadays. In fact, we mentioned that bacteria would become very handy, helpful and intelligent “machines” in appropriate conditions. We listed the former experiments and parts that took place in iGEM competition and gave some examples about what to do next. With this article lots of people informed and their some negative sentiments about the microorganisms and synthetic biology would be changed.<br />
<br />
4) We have prepared a journal which is named “Canvas Times” and it gives you the actual news of our lab and team. Also you can learn about our project and read our columnist and you can travel our peace and quiet town the Canvas Town. “Canvas Times” a new journal of young scientists; it gives you the actual news of our lab and team. <br />
During our lab working, we could find some time to fun and talk with people about our project, synthetic biology and iGEM... Our all of activities and human practices are existed on our journal such as “iGEM Picnic” and “International Medical Students Conference. Therewithal you will find some information of our university and faculty. You can find it all of stores.<br />
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2011-09-22T01:15:54Z
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<h2 style="font-family: Verdana, Arial, SunSans-Regular, sans-serif;">Human Practice</h2><br />
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<tr><td>To remove infections B.subtilis spores from our Anti-LPS factor containing biofilm paste, fenton reagent is used before use against E.coli.</td></tr><br />
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<tr><td>During funny summer in our project executions, we have also met hundreds of people from different ages in order to tell our project in iGEM competition and benefits of synthetic biology.</td></tr><br />
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<tr><td>We have designed monopoly like game name "The Canvas Town" to inform people from any distinct education level about features of our project, laboratory equipments and interesting Biobrick of iGEM competition.</td></tr><br />
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<tr><td>a new journal of young scientists; it gives you the actual news of our lab and team</td></tr><br />
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<tr><td>During funny summer in our project executions, we have also met hundreds of people from different ages in order to tell our project in iGEM competition and benefits of synthetic biology.</td></tr><br />
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Metugen
http://2011.igem.org/Team:Fatih_Turkey/Human_Practice
Team:Fatih Turkey/Human Practice
2011-09-22T01:15:29Z
<p>Metugen: </p>
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<td><h2><a href="https://2011.igem.org/Team:Fatih_Turkey/Sporocide">Sporocide</a></h2></td><br />
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<tr><td>To remove infections B.subtilis spores from our Anti-LPS factor containing biofilm paste, fenton reagent is used before use against E.coli.</td></tr><br />
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<tr><td>During funny summer in our project executions, we have also met hundreds of people from different ages in order to tell our project in iGEM competition and benefits of synthetic biology.</td></tr><br />
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<td><h2><a href="https://2011.igem.org/Team:Fatih_Turkey/game">Game</a></h2></td><br />
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<tr><td>We have designed monopoly like game name "The Canvas Town" to inform people from any distinct education level about features of our project, laboratory equipments and interesting Biobrick of iGEM competition.</td></tr><br />
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<td><h2><a href="https://2011.igem.org/Team:Fatih_Turkey/canvas_times">Canvas Times</a></h2></td><br />
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<tr><td>a new journal of young scientists; it gives you the actual news of our lab and team</td></tr><br />
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<tr><td>During funny summer in our project executions, we have also met hundreds of people from different ages in order to tell our project in iGEM competition and benefits of synthetic biology.</td></tr><br />
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Metugen
http://2011.igem.org/Gamehp.html
Gamehp.html
2011-07-23T20:49:57Z
<p>Metugen: </p>
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<td width="203" align="center" valign="middle" bgcolor="#990000"><a href="https://2011.igem.org/Team:Fatih_Turkey"><span class="style3">HOME</span></a></td><br />
<td width="228" align="center" valign="middle" bgcolor="#695E0E"><a href="canvastown.html"><span class="style3">CANVAS&nbsp; TOWN</span></a></td><br />
<td width="277" align="center" valign="middle" bgcolor="#1D3551"><a href="therainbowgraveyard.html"><span class="style3">THE RAINBOW GRAVEYARD</span></a></td><br />
<td width="192" align="center" valign="middle" bgcolor="#960C47"><a href="notebook.html"><span class="style3">&nbsp;NOTEBOOK</span></a></td><br />
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<td height="20" colspan="4" align="center" valign="middle" bgcolor="#695E0E"><span class="style3">GAME</span></td><br />
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<tr><br />
<td height="555" align="center" valign="top" bgcolor="#FFFFFF"><p><img src="https://static.igem.org/mediawiki/2011/c/c0/DSC03393.JPG" alt="" width="640" height="480" /></p><br />
<p>Canvas Town game can be played with your friends, your labmates and with other iGEM teams as a competition on Synthetic biology `Monopoly-like` game.</p><br />
<p>-------------------------------------------------------------------------------------------------------------------------------------</p><br />
<p>&nbsp;</p><br />
<p><img src="https://static.igem.org/mediawiki/2011/2/26/DSC03372.JPG" width="640" height="480" /></p><br />
<p>Despite the struggle of all players in Canvas Town Game, they are still good friends... From right to left, B.subtilis, Virus and E.coli...</p><br />
<p>-------------------------------------------------------------------------------------------------------------------------------------- </p><br />
<p><img src="https://static.igem.org/mediawiki/2011/e/e2/DSC03282.JPG" width="640" height="480" /></p><br />
<p>Players (Virus,B.subtilis,E.coli) are ready to go through dangerous ways of Canvas Town.</p><br />
<p>-----------------------------------------------------------------------------------------------------------------------------------</p><br />
<p><img src="https://static.igem.org/mediawiki/2011/1/17/DSC03290.JPG" width="640" height="480" /></p><br />
<p>After character cards distributed to players, our team member Fazilet laughes the situation of other player, whom has disadvantages.</p><br />
<p>------------------------------------------------------------------------------------------------------------------------------------<br /><br />
</p><br />
<p><img src="https://static.igem.org/mediawiki/2011/8/8c/DSC03292.JPG" width="640" height="480" /></p><br />
<p><br /><br />
Virus team in the game, Mustafa and Fazilet shares their distinct cards in the same box during the game. </p><br />
<p>---------------------------------------------------------------------------------------------------------------------------------</p><br />
<p><img src="https://static.igem.org/mediawiki/2011/3/3d/DSC03301.JPG" width="640" height="480" /></p><br />
<p>Virus team is relax in Alcohol sterilized box because viruses can withstand.<br /><br />
<br /><br />
----------------------------------------------------------------------------------------------------------------------------------<br />
</p><br />
<p><img src="https://static.igem.org/mediawiki/2011/1/14/DSC03304.JPG" width="640" height="480" /></p><br />
<p>After rolling of dice, E.coli goes forward.</p><br />
<p>----------------------------------------------------------------------------------------------------------------------------------</p><br />
<p><img src="https://static.igem.org/mediawiki/2011/5/5a/DSC03352.JPG" width="640" height="480" /></p><br />
<p>Alperen, other E.coli player, laughes but his E.coli team's situation is very bad because both of Cihan and Alperen are at START point.</p><br />
<p>------------------------------------------------------------------------------------------------------------------------------------- </p><br />
<p><img src="https://static.igem.org/mediawiki/2011/4/41/DSC03305.JPG" width="640" height="480" /></p><br />
<p><br /><br />
Situation of Cihan in the game is bad since E.coli is again and again killed in Autoclav box.</p><br />
<p>-------------------------------------------------------------------------------------------------------------------------------------</p><br />
<p><img src="https://static.igem.org/mediawiki/2011/b/ba/DSC03310.JPG" width="640" height="480" /></p><br />
<p>Mustafa comes to Gene Synthesizer box and selects new card from Gene synthesizer cards.</p><br />
<p>------------------------------------------------------------------------------------------------------------------------------------</p><br />
<p><img src="https://static.igem.org/mediawiki/2011/2/23/DSC03300.JPG" width="640" height="480" /></p><br />
<p>E.coli and B.subtilis are still at the START position inspite of several cycles of rolling dice.</p><br />
<p>------------------------------------------------------------------------------------------------------------------------------------</p><br />
<p>&nbsp;</p><br />
<p><img src="https://static.igem.org/mediawiki/2011/9/91/DSC03314.JPG" width="640" height="480" /></p><br />
<p><br /><br />
Mustafa, virus player, comes to UV box and its DNA is disrupted. Thus, goes START point.<br /><br />
------------------------------------------------------------------------------------------------------------------------------------<br />
</p><br />
<p><img src="https://static.igem.org/mediawiki/2011/7/74/DSC03315.JPG" width="640" height="480" /></p><br />
<p>Very thoughtfull time, the players think their destiny in Canvas Town. `To be or not to be, that's the point!`</p><br />
<p>-------------------------------------------------------------------------------------------------------------------------------------</p><br />
<p><img src="https://static.igem.org/mediawiki/2011/8/81/DSC03319.JPG" width="640" height="480" /></p><br />
<p>&nbsp;</p><br />
<p><img src="https://static.igem.org/mediawiki/2011/7/76/DSC03320.JPG" width="640" height="853" /></p><br />
<p><br /><br />
Top view of all Fatih Turkey team while playing the game. What an exciting game!</p><br />
<p>-------------------------------------------------------------------------------------------------------------------------------------- </p><br />
<p><img src="https://static.igem.org/mediawiki/2011/5/56/DSC03328.JPG" width="640" height="480" /></p><br />
<p><br /><br />
Ozan, B.subtilis player, rolls dice! Rolling to dead:)</p><br />
<p>-----------------------------------------------------------------------------------------------------------------------------------</p><br />
<p><img src="https://static.igem.org/mediawiki/2011/d/d6/DSC03330.JPG" width="640" height="480" /></p><br />
<p>Tulin, B.subtilis player, is laughing because she is again killed in kanamycin box due to absence of the resistance.</p><br />
<p>-------------------------------------------------------------------------------------------------------------------------------------<br /><br />
</p><br />
<p><img src="https://static.igem.org/mediawiki/2011/9/9e/DSC03340.JPG" width="640" height="480" /></p><br />
<p>Again and again... Cihan, E.coli player, is in START box even at the end of tens of cycle. Very unlucky person...</p><br />
<p>------------------------------------------------------------------------------------------------------------------------------------</p><br />
<p><img src="https://static.igem.org/mediawiki/2011/a/a3/DSC03345.JPG" width="640" height="480" /></p><br />
<p><br /><br />
The good time for Cihan because he goes three steps forward.</p><br />
<p>------------------------------------------------------------------------------------------------------------------------------------</p><br />
<p><img src="https://static.igem.org/mediawiki/2011/2/29/DSC03329.JPG" width="640" height="480" /></p><br />
<p>Ozan is very happy like reaching to Nirvana! Because he is still alive and close to END.</p><br />
<p>------------------------------------------------------------------------------------------------------------------------------------</p><br />
<p><img src="https://static.igem.org/mediawiki/2011/9/92/DSC03365.JPG" width="640" height="480" /></p><br />
<p>Looking from the edge of Convas Town Box, in which players can go forward if they have TAKE A PHOTO character card.</p><br />
<p>-------------------------------------------------------------------------------------------------------------------------------------- </p><br />
<p><img src="https://static.igem.org/mediawiki/2011/a/a8/DSC03380.JPG" width="640" height="480" /></p><br />
<p>Oooo! B.subtilis comes to Pst1 restriction site box, here if any player has a Pst1 site on its genome, then it is killed and returned to near check point... Or if the player has Pst1 sites on the character plasmids, then the player losts the character card.</p><br />
<p>------------------------------------------------------------------------------------------------------------------------------------- </p><br />
<p><img src="https://static.igem.org/mediawiki/2011/0/01/DSC03381.JPG" width="640" height="480" /></p><br />
<p>Virus and E.coli are at Fatih Turkey Lab Box;that is, they are too close to final box `iGEM` after passing Holland Box...</p><br />
<p>-------------------------------------------------------------------------------------------------------------------------------------- </p><br />
<p><img src="https://static.igem.org/mediawiki/2011/1/1d/DSC03384.JPG" width="640" height="480" /></p><br />
<p>Canvas Town Game has been designed like that any person can play and learn very useful information about organisms, techniques and devices used mostly in a synthetic biology lab and about iGEM interesting Biobrick parts.</p><br />
<p>------------------------------------------------------------------------------------------------------------------------------------ </p><br />
<p><img src="https://static.igem.org/mediawiki/2011/9/96/DSC03388.JPG" width="640" height="480" /></p><br />
<p>The winner in our game was Virus team by reaching to the END first. E.coli and B.subtilis shares second and third positions according to their situations.</p><br />
<p>--------------------------------------------------------------------------------------------------------------------------------------<br /></p><br />
<p><img src="https://static.igem.org/mediawiki/2011/a/ae/DSC03389.JPG" width="640" height="480" /></p><br />
<p>In Canvas Town game, when players come to Gene Synthesizer Box, then they can take a card from Gene synthesis place.</p><br />
<p>------------------------------------------------------------------------------------------------------------------------------------- </p><br />
<p><img src="https://static.igem.org/mediawiki/2011/7/74/DSC03400.JPG" alt="" width="640" height="480" /></p><br />
<p>You can give Canvas Town Game set to your friends, families and people in your city, especially students from primary school to high school and even in universities.</p><br />
<p>--------------------------------------------------------------------------------------------------------------------------------------</p><br />
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<td align="center" valign="top" bgcolor="#586877"><a href="gamehp.html">Back to TOP!</a></td><br />
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Metugen
http://2011.igem.org/Notebook.html
Notebook.html
2011-07-22T18:25:11Z
<p>Metugen: </p>
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Metugen
http://2011.igem.org/Notebook.html
Notebook.html
2011-07-22T18:20:31Z
<p>Metugen: </p>
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Metugen
http://2011.igem.org/Notebook.html
Notebook.html
2011-07-22T18:19:33Z
<p>Metugen: </p>
<hr />
<div><html><iframe src="https://www.google.com/calendar/embed?height=600&amp;wkst=1&amp;hl=en&amp;bgcolor=%23cc33cc&amp;src=j3om93lpmd2quea5cv67n5ooqg%40group.calendar.google.com&amp;color=%23182C57&amp;ctz=Europe%2FIstanbul" style=" border-width:0 " width="800" height="600" frameborder="0" scrolling="no"></iframe></html></div>
Metugen
http://2011.igem.org/Notebook.html
Notebook.html
2011-07-22T18:19:04Z
<p>Metugen: </p>
<hr />
<div><iframe src="https://www.google.com/calendar/embed?height=600&amp;wkst=1&amp;hl=en&amp;bgcolor=%23cc33cc&amp;src=j3om93lpmd2quea5cv67n5ooqg%40group.calendar.google.com&amp;color=%23182C57&amp;ctz=Europe%2FIstanbul" style=" border-width:0 " width="800" height="600" frameborder="0" scrolling="no"></iframe></div>
Metugen