http://2011.igem.org/wiki/index.php?title=Special:Contributions&feed=atom&limit=50&target=Cambi&year=&month=2011.igem.org - User contributions [en]2024-03-28T19:32:07ZFrom 2011.igem.orgMediaWiki 1.16.0http://2011.igem.org/Team:SYSU-China/page_aboutus_team_membersTeam:SYSU-China/page aboutus team members2011-10-28T16:11:48Z<p>Cambi: </p>
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<li><a href="https://2011.igem.org/Team:SYSU-China"><span></span></a></li><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/main_page_news"><span>NEWS</span></a></li><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/main_page_story"><span>STORY</span></a></li> <br />
<li><a href="https://2011.igem.org/Team:SYSU-China/main_page_project" class="parent"><span>PROJECT</span></a><br />
<div><br />
<ul><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/project_bacterial_migration"><span>Bacterial Migration</span></a><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/project_Cesium_Absorption"><span>Cesium Absorption</span></a></li><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/project_Aggregation_Recovery"><span>Aggregation & Recovery</span></a></li><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/page_project_notes"><span>Notes</span></a></li><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/page_project_datapage"><span>Data Page</span></a></li><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/page_project_safety"><span>Safety</span></a></li><br />
</ul><br />
</div><br />
</li><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/main_page_human_practice" class="parent"><span>HUMAN PRACTICE</span></a><br />
<div><br />
<ul><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/page_human_practice_app"><span>iOS App</span></a><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/page_human_practice_labcraft"><span>LabCraft Board Game</span></a></li><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/page_human_practice_workshop"><span>Workshop</span></a></li><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/page_human_practice_survey"><span>Survey</span></a></li><br />
</ul><br />
</div><br />
</li><br />
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<li><a href="https://2011.igem.org/Team:SYSU-China/main_page_aboutus" class="parent"><span>ABOUT US</span></a><br />
<div><br />
<ul><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/page_aboutus_team_members"><span>Team Members</span></a><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/page_aboutus_special_thanks"><span>Special Thanks</span></a></li><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/page_aboutus_LT"><span>Visual Identity</span></a></li><br />
</ul><br />
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</li><br />
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<div id="logo_bar"><a href="https://2011.igem.org/Team:SYSU-China"><img src="https://static.igem.org/mediawiki/2011/2/25/Logo_green.png" width="93" height="125" /></a></div><br />
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<div id="page_content_baBar_details"><a href="#page_content_textBar_enterTEXT">See Details</a></div><br />
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<div class="page_content_aboutus_textBar"><br />
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<ul><br />
<li><img name="" src="https://static.igem.org/mediawiki/2011/5/5d/2011_10_04_iGEM_SYSU_%E9%9B%86%E4%BD%93%E7%85%A7093.jpg" width="114" height="152" alt="" /><br />
<h1>Li WANG</h1><br />
<p>Western Bloter</p><br />
</li><br />
<li><img name="" src="https://static.igem.org/mediawiki/2011/1/10/2011_10_04_iGEM_SYSU_%E9%9B%86%E4%BD%93%E7%85%A7123.jpg" width="114" height="152" alt="" /><br />
<h1>Qiang KOU</h1><br />
<p>Modeler 模特儿</li><br />
<li><img name="" src="https://static.igem.org/mediawiki/2011/9/9a/2011_10_04_iGEM_SYSU_%E9%9B%86%E4%BD%93%E7%85%A7067.jpg" width="114" height="152" alt="" /><br />
<h1>Tao GUO</h1><br />
<p>iPhone app coder </p><br />
</li><br />
<li><img name="" src="https://static.igem.org/mediawiki/2011/0/00/2011_10_04_iGEM_SYSU_%E9%9B%86%E4%BD%93%E7%85%A7083.jpg" width="114" height="152" alt="" /><br />
<h1>WeiWen SUN</h1><br />
<p>TOEFLer</p><br />
</li><br />
<li><img name="" src="https://static.igem.org/mediawiki/2011/9/93/2011_10_04_iGEM_SYSU_%E9%9B%86%E4%BD%93%E7%85%A7077.jpg" width="114" height="152" alt="" /><br />
<h1>ZiLong WANG</h1><br />
<p>Leader</p><br />
</li><br />
<li><img name="" src="https://static.igem.org/mediawiki/2011/8/80/2011_10_04_iGEM_SYSU_%E9%9B%86%E4%BD%93%E7%85%A7100.jpg" width="114" height="152" alt="" /><br />
<h1>Yi ZHENG</h1><br />
<p>Experiemntalist & Fantastic Dancer</p><br />
<p><br />
</li><br />
<li><img name="" src="https://static.igem.org/mediawiki/2011/7/7c/2011_10_04_iGEM_SYSU_%E9%9B%86%E4%BD%93%E7%85%A7112.jpg" width="114" height="152" alt="" /><br />
<h1>YingJie LIU</h1><br />
<p>Coder, and superisingly, a girl......</p><br />
</li><br />
<li><img name="" src="https://static.igem.org/mediawiki/2011/0/0b/2011_10_04_iGEM_SYSU_%E9%9B%86%E4%BD%93%E7%85%A7082.jpg" width="114" height="152" alt="" /><br />
<h1>YiTian XU</h1><br />
<p>Big fan of Big Bang Theory!! The TV of course!! Experiemntalist ><</p><br />
</li><br />
<li><img name="" src="https://static.igem.org/mediawiki/2011/e/ed/2011_10_04_iGEM_SYSU_%E9%9B%86%E4%BD%93%E7%85%A7130.jpg" width="114" height="152" alt="" /><br />
<h1>Garfield ZHAO</h1><br />
<p>Experiemntalist & Wiki Coder &amp; a man's man... &amp; not a professional TOEFLer or GREer...</p><br />
</li><br />
<li><img name="" src="https://static.igem.org/mediawiki/2011/9/9e/2011_10_04_iGEM_SYSU_%E9%9B%86%E4%BD%93%E7%85%A7141.jpg" width="114" height="152" alt="" /><br />
<h1>WenJun SU</h1><br />
<p>Vision Superviser & a little cub ... yes,a little cub indeed...<br />
</li><br />
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</ul><br />
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</div> <br />
</div><br />
</div><br />
<!---------------------------------------------!--><br />
<div id="page_content_RsideBar"><br />
<div class="page_content_relatedBar_aboutus"><br />
<h2>Check our Renren pages:</h2><br />
<h1><a href="http://www.renren.com/profile.do?id=398865894&q=%E7%8E%8B%E5%8A%9B&p=&s=&u=250313171&act=name&rt=user&in=5&ft=2&hh=1">Li Wang's Renren</a></h1><br />
<h1><a href="http://www.renren.com/profile.do?id=268292351&q=%E5%AF%87%E5%BC%BA&p=&s=&u=250313171&act=head&rt=user&in=0&ft=1&hh=1">Qiang Kou's Renren</a></h1><br />
<h1><a href="http://www.renren.com/profile.do?id=243854154&q=%E9%83%AD%E6%B6%9B&p=&s=&u=250313171&act=head&rt=user&in=2&ft=1&hh=1">Tao Guo's Renren</a></h1><br />
<h1><a href="http://www.renren.com/profile.do?id=248684166&q=%E5%AD%99%E5%A8%81%E6%96%87&p=&s=&u=250313171&act=head&rt=user&in=0&ft=1&hh=1">Weiwen Sun's Renren</a></h1><br />
<h1><a href="http://www.renren.com/profile.do?id=265056277&q=%E7%8E%8B%E5%AD%90%E9%BE%99&p=&s=open&u=250313171&act=head&rt=user&in=1&ft=1&hh=1">Zilong Wang's Renren</a></h1><br />
<h1><a href="http://www.renren.com/profile.do?id=248483845&q=%E9%83%91%E6%AF%85&p=&s=&u=250313171&act=head&rt=user&in=0&ft=1&hh=1">Yi Zheng's Renren</a></h1><br />
<h1><a href="http://www.renren.com/profile.do?id=282370064&q=%E5%88%98%E9%A2%96%E6%B4%81&p=&s=&u=250313171&act=head&rt=user&in=0&ft=1&hh=1">Yingjie Liu's Renren</a></h1><br />
<h1><a href="http://www.renren.com/profile.do?id=250313171&from=opensearch">Garfield Zhao's Renren</a></h1><br />
<h1><a href="http://www.renren.com/profile.do?id=256143356&q=%E8%8B%8F%E6%96%87%E9%AA%8F&p=&s=&u=250313171&act=head&rt=user&in=0&ft=1&hh=1">Wenjun Su's RenRen</a></h1><br />
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<p>Note for non-Chinese:</p><br /><br />
<p>&nbsp;&nbsp;&nbsp;&nbsp;Renren is "Chinalized" Facebook. Normally, we can not get access to Facebook or Twitter because of you-know-why....</p><br />
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<!--here begins the contact_bottombar!--><!-- #BeginLibraryItem "/Library/contact_bottombar.lbi" --><div class="contact_bottombar"><br />
<p><a href="https://2011.igem.org/Main_Page">2011 iGEM Mainpage</a></p><br />
<p><a href="https://igem.org/Team_Wikis?year=2011">2011 iGEM Team wikis</a></p><br />
<br />
<p>From the 2011 iGEM team SYSU-China (2011)</p><br />
<p>Sun Yat-Sen University, Guangzhou, China</p><br />
<p>Address: 135# Xingang Rd.(W.), Haizhu Guangzhou, P.R.China</p><br />
<p><a href="http://eng.sysu.edu.cn/">visit the Sun Yat-sen university website</a></p><br />
<p>Thanks Apycom jQuery Menus and <a href="http://apycom.com/">visit their website</a><br />
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</html></div>Cambihttp://2011.igem.org/Team:SYSU-China/page_aboutus_team_membersTeam:SYSU-China/page aboutus team members2011-10-28T16:10:33Z<p>Cambi: </p>
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<ul class="menu"><br />
<li><a href="https://2011.igem.org/Team:SYSU-China"><span></span></a></li><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/main_page_news"><span>NEWS</span></a></li><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/main_page_story"><span>STORY</span></a></li> <br />
<li><a href="https://2011.igem.org/Team:SYSU-China/main_page_project" class="parent"><span>PROJECT</span></a><br />
<div><br />
<ul><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/project_bacterial_migration"><span>Bacterial Migration</span></a><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/project_Cesium_Absorption"><span>Cesium Absorption</span></a></li><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/project_Aggregation_Recovery"><span>Aggregation & Recovery</span></a></li><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/page_project_notes"><span>Notes</span></a></li><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/page_project_datapage"><span>Data Page</span></a></li><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/page_project_safety"><span>Safety</span></a></li><br />
</ul><br />
</div><br />
</li><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/main_page_human_practice" class="parent"><span>HUMAN PRACTICE</span></a><br />
<div><br />
<ul><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/page_human_practice_app"><span>iOS App</span></a><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/page_human_practice_labcraft"><span>LabCraft Board Game</span></a></li><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/page_human_practice_workshop"><span>Workshop</span></a></li><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/page_human_practice_survey"><span>Survey</span></a></li><br />
</ul><br />
</div><br />
</li><br />
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<li><a href="https://2011.igem.org/Team:SYSU-China/main_page_aboutus" class="parent"><span>ABOUT US</span></a><br />
<div><br />
<ul><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/page_aboutus_team_members"><span>Team Members</span></a><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/page_aboutus_special_thanks"><span>Special Thanks</span></a></li><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/page_aboutus_LT"><span>Visual Identity</span></a></li><br />
</ul><br />
</div><br />
</li><br />
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</ul><br />
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<div id="page_content_baBar_details"><a href="#page_content_textBar_enterTEXT">See Details</a></div><br />
</div><br />
<div class="page_content_aboutus_textBar"><br />
<div id="page_content_textBar_enterTEXT"><br />
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<ul><br />
<li><img name="" src="https://static.igem.org/mediawiki/2011/5/5d/2011_10_04_iGEM_SYSU_%E9%9B%86%E4%BD%93%E7%85%A7093.jpg" width="114" height="152" alt="" /><br />
<h1>Li WANG</h1><br />
<p>Western Bloter</p><br />
</li><br />
<li><img name="" src="https://static.igem.org/mediawiki/2011/1/10/2011_10_04_iGEM_SYSU_%E9%9B%86%E4%BD%93%E7%85%A7123.jpg" width="114" height="152" alt="" /><br />
<h1>Qiang KOU</h1><br />
<p>Modeler 模特儿</li><br />
<li><img name="" src="https://static.igem.org/mediawiki/2011/9/9a/2011_10_04_iGEM_SYSU_%E9%9B%86%E4%BD%93%E7%85%A7067.jpg" width="114" height="152" alt="" /><br />
<h1>Tao GUO</h1><br />
<p>The one brings milk </p><br />
</li><br />
<li><img name="" src="https://static.igem.org/mediawiki/2011/0/00/2011_10_04_iGEM_SYSU_%E9%9B%86%E4%BD%93%E7%85%A7083.jpg" width="114" height="152" alt="" /><br />
<h1>WeiWen SUN</h1><br />
<p>TOEFLer</p><br />
</li><br />
<li><img name="" src="https://static.igem.org/mediawiki/2011/9/93/2011_10_04_iGEM_SYSU_%E9%9B%86%E4%BD%93%E7%85%A7077.jpg" width="114" height="152" alt="" /><br />
<h1>ZiLong WANG</h1><br />
<p>Leader</p><br />
</li><br />
<li><img name="" src="https://static.igem.org/mediawiki/2011/8/80/2011_10_04_iGEM_SYSU_%E9%9B%86%E4%BD%93%E7%85%A7100.jpg" width="114" height="152" alt="" /><br />
<h1>Yi ZHENG</h1><br />
<p>Experiemntalist & Fantastic Dancer</p><br />
<p><br />
</li><br />
<li><img name="" src="https://static.igem.org/mediawiki/2011/7/7c/2011_10_04_iGEM_SYSU_%E9%9B%86%E4%BD%93%E7%85%A7112.jpg" width="114" height="152" alt="" /><br />
<h1>YingJie LIU</h1><br />
<p>Coder, and superisingly, a girl......</p><br />
</li><br />
<li><img name="" src="https://static.igem.org/mediawiki/2011/0/0b/2011_10_04_iGEM_SYSU_%E9%9B%86%E4%BD%93%E7%85%A7082.jpg" width="114" height="152" alt="" /><br />
<h1>YiTian XU</h1><br />
<p>Big fan of Big Bang Theory!! The TV of course!! Experiemntalist ><</p><br />
</li><br />
<li><img name="" src="https://static.igem.org/mediawiki/2011/e/ed/2011_10_04_iGEM_SYSU_%E9%9B%86%E4%BD%93%E7%85%A7130.jpg" width="114" height="152" alt="" /><br />
<h1>Garfield ZHAO</h1><br />
<p>Experiemntalist & Wiki Coder &amp; a man's man... &amp; not a professional TOEFLer or GREer...</p><br />
</li><br />
<li><img name="" src="https://static.igem.org/mediawiki/2011/9/9e/2011_10_04_iGEM_SYSU_%E9%9B%86%E4%BD%93%E7%85%A7141.jpg" width="114" height="152" alt="" /><br />
<h1>WenJun SU</h1><br />
<p>Vision Superviser & a little cub ... yes,a little cub indeed...<br />
</li><br />
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</ul><br />
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</div><br />
</div><br />
<!---------------------------------------------!--><br />
<div id="page_content_RsideBar"><br />
<div class="page_content_relatedBar_aboutus"><br />
<h2>Check our Renren pages:</h2><br />
<h1><a href="http://www.renren.com/profile.do?id=398865894&q=%E7%8E%8B%E5%8A%9B&p=&s=&u=250313171&act=name&rt=user&in=5&ft=2&hh=1">Li Wang's Renren</a></h1><br />
<h1><a href="http://www.renren.com/profile.do?id=268292351&q=%E5%AF%87%E5%BC%BA&p=&s=&u=250313171&act=head&rt=user&in=0&ft=1&hh=1">Qiang Kou's Renren</a></h1><br />
<h1><a href="http://www.renren.com/profile.do?id=243854154&q=%E9%83%AD%E6%B6%9B&p=&s=&u=250313171&act=head&rt=user&in=2&ft=1&hh=1">Tao Guo's Renren</a></h1><br />
<h1><a href="http://www.renren.com/profile.do?id=248684166&q=%E5%AD%99%E5%A8%81%E6%96%87&p=&s=&u=250313171&act=head&rt=user&in=0&ft=1&hh=1">Weiwen Sun's Renren</a></h1><br />
<h1><a href="http://www.renren.com/profile.do?id=265056277&q=%E7%8E%8B%E5%AD%90%E9%BE%99&p=&s=open&u=250313171&act=head&rt=user&in=1&ft=1&hh=1">Zilong Wang's Renren</a></h1><br />
<h1><a href="http://www.renren.com/profile.do?id=248483845&q=%E9%83%91%E6%AF%85&p=&s=&u=250313171&act=head&rt=user&in=0&ft=1&hh=1">Yi Zheng's Renren</a></h1><br />
<h1><a href="http://www.renren.com/profile.do?id=282370064&q=%E5%88%98%E9%A2%96%E6%B4%81&p=&s=&u=250313171&act=head&rt=user&in=0&ft=1&hh=1">Yingjie Liu's Renren</a></h1><br />
<h1><a href="http://www.renren.com/profile.do?id=250313171&from=opensearch">Garfield Zhao's Renren</a></h1><br />
<h1><a href="http://www.renren.com/profile.do?id=256143356&q=%E8%8B%8F%E6%96%87%E9%AA%8F&p=&s=&u=250313171&act=head&rt=user&in=0&ft=1&hh=1">Wenjun Su's RenRen</a></h1><br />
<br /><br />
<p>Note for non-Chinese:</p><br /><br />
<p>&nbsp;&nbsp;&nbsp;&nbsp;Renren is "Chinalized" Facebook. Normally, we can not get access to Facebook or Twitter because of you-know-why....</p><br />
</div><br />
</div> <br />
<!---------------------------------------------!--><br />
<div class="main_page_clearer_light"><br />
</div><br />
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</div><!--here ends the page_content_WholeBox!--><br />
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<br />
<!--here begins the contact_bottombar!--><!-- #BeginLibraryItem "/Library/contact_bottombar.lbi" --><div class="contact_bottombar"><br />
<p><a href="https://2011.igem.org/Main_Page">2011 iGEM Mainpage</a></p><br />
<p><a href="https://igem.org/Team_Wikis?year=2011">2011 iGEM Team wikis</a></p><br />
<br />
<p>From the 2011 iGEM team SYSU-China (2011)</p><br />
<p>Sun Yat-Sen University, Guangzhou, China</p><br />
<p>Address: 135# Xingang Rd.(W.), Haizhu Guangzhou, P.R.China</p><br />
<p><a href="http://eng.sysu.edu.cn/">visit the Sun Yat-sen university website</a></p><br />
<p>Thanks Apycom jQuery Menus and <a href="http://apycom.com/">visit their website</a><br />
</div><!-- #EndLibraryItem --><!--here ends the contact_bottombar!--><br />
<br />
</div><!--here ends the backgroud_shadow_bar!--><br />
</body><br />
</html></div>Cambihttp://2011.igem.org/Team:SYSU-China/page_aboutus_team_membersTeam:SYSU-China/page aboutus team members2011-10-28T16:10:06Z<p>Cambi: </p>
<hr />
<div>{{:Team:SYSU-China/header/wikireset}}<br />
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<meta http-equiv="Content-Type" content="text/html; charset=utf-8" /><br />
<title>Team Members-Sun Yat-sen Univ.</title><br />
<link type="text/css" href="menu.css" rel="stylesheet" /><br />
<script type="text/javascript" src="jquery.js"></script><br />
<script type="text/javascript" src="menu.js"></script><br />
<script type="text/javascript" src="scrollTo.js"></script><br />
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<!--------here begins the manu bar section--!--><!-- #BeginLibraryItem "/Library/manu.page.lbi" --><!-- #BeginLibraryItem "/Library/manu.lbi" --><!--------here begins the manu bar section--!--><br />
<div class="title_container"><br />
<div id="menu"><br />
<ul class="menu"><br />
<li><a href="https://2011.igem.org/Team:SYSU-China"><span></span></a></li><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/main_page_news"><span>NEWS</span></a></li><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/main_page_story"><span>STORY</span></a></li> <br />
<li><a href="https://2011.igem.org/Team:SYSU-China/main_page_project" class="parent"><span>PROJECT</span></a><br />
<div><br />
<ul><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/project_bacterial_migration"><span>Bacterial Migration</span></a><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/project_Cesium_Absorption"><span>Cesium Absorption</span></a></li><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/project_Aggregation_Recovery"><span>Aggregation & Recovery</span></a></li><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/page_project_notes"><span>Notes</span></a></li><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/page_project_datapage"><span>Data Page</span></a></li><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/page_project_safety"><span>Safety</span></a></li><br />
</ul><br />
</div><br />
</li><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/main_page_human_practice" class="parent"><span>HUMAN PRACTICE</span></a><br />
<div><br />
<ul><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/page_human_practice_app"><span>iOS App</span></a><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/page_human_practice_labcraft"><span>LabCraft Board Game</span></a></li><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/page_human_practice_workshop"><span>Workshop</span></a></li><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/page_human_practice_survey"><span>Survey</span></a></li><br />
</ul><br />
</div><br />
</li><br />
<br />
<li><a href="https://2011.igem.org/Team:SYSU-China/main_page_aboutus" class="parent"><span>ABOUT US</span></a><br />
<div><br />
<ul><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/page_aboutus_team_members"><span>Team Members</span></a><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/page_aboutus_special_thanks"><span>Special Thanks</span></a></li><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/page_aboutus_LT"><span>Visual Identity</span></a></li><br />
</ul><br />
</div><br />
</li><br />
<br />
</ul><br />
</div><!--here ends the manu!--><br />
<div id="logo_bar"><a href="https://2011.igem.org/Team:SYSU-China"><img src="https://static.igem.org/mediawiki/2011/2/25/Logo_green.png" width="93" height="125" /></a></div><br />
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<div class="page_clear_and_introBar"><br />
<img src="https://static.igem.org/mediawiki/igem.org/0/09/SYSU_Page_aboutus_team_members_introBar.png<br />
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<div id="page_content_WholeBox"><br />
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<div class="page_content_bgBar"><img src="https://static.igem.org/mediawiki/2011/9/9d/Pagehead_tm.jpg" /><br />
<div id="page_content_baBar_details"><a href="#page_content_textBar_enterTEXT">See Details</a></div><br />
</div><br />
<div class="page_content_aboutus_textBar"><br />
<div id="page_content_textBar_enterTEXT"><br />
<br />
<ul><br />
<li><img name="" src="https://static.igem.org/mediawiki/2011/5/5d/2011_10_04_iGEM_SYSU_%E9%9B%86%E4%BD%93%E7%85%A7093.jpg" width="114" height="152" alt="" /><br />
<h1>Li WANG</h1><br />
<p>Western Bloter</p><br />
</li><br />
<li><img name="" src="https://static.igem.org/mediawiki/2011/1/10/2011_10_04_iGEM_SYSU_%E9%9B%86%E4%BD%93%E7%85%A7123.jpg" width="114" height="152" alt="" /><br />
<h1>Qiang KOU</h1><br />
<p>Modeler 模特儿</li><br />
<li><img name="" src="https://static.igem.org/mediawiki/2011/9/9a/2011_10_04_iGEM_SYSU_%E9%9B%86%E4%BD%93%E7%85%A7067.jpg" width="114" height="152" alt="" /><br />
<h1>Tao GUO</h1><br />
<p>The one brings milk... </p><br />
</li><br />
<li><img name="" src="https://static.igem.org/mediawiki/2011/0/00/2011_10_04_iGEM_SYSU_%E9%9B%86%E4%BD%93%E7%85%A7083.jpg" width="114" height="152" alt="" /><br />
<h1>WeiWen SUN</h1><br />
<p>TOEFLer</p><br />
</li><br />
<li><img name="" src="https://static.igem.org/mediawiki/2011/9/93/2011_10_04_iGEM_SYSU_%E9%9B%86%E4%BD%93%E7%85%A7077.jpg" width="114" height="152" alt="" /><br />
<h1>ZiLong WANG</h1><br />
<p>Leader</p><br />
</li><br />
<li><img name="" src="https://static.igem.org/mediawiki/2011/8/80/2011_10_04_iGEM_SYSU_%E9%9B%86%E4%BD%93%E7%85%A7100.jpg" width="114" height="152" alt="" /><br />
<h1>Yi ZHENG</h1><br />
<p>Experiemntalist & Fantastic Dancer</p><br />
<p><br />
</li><br />
<li><img name="" src="https://static.igem.org/mediawiki/2011/7/7c/2011_10_04_iGEM_SYSU_%E9%9B%86%E4%BD%93%E7%85%A7112.jpg" width="114" height="152" alt="" /><br />
<h1>YingJie LIU</h1><br />
<p>Coder, and superisingly, a girl......</p><br />
</li><br />
<li><img name="" src="https://static.igem.org/mediawiki/2011/0/0b/2011_10_04_iGEM_SYSU_%E9%9B%86%E4%BD%93%E7%85%A7082.jpg" width="114" height="152" alt="" /><br />
<h1>YiTian XU</h1><br />
<p>Big fan of Big Bang Theory!! The TV of course!! Experiemntalist ><</p><br />
</li><br />
<li><img name="" src="https://static.igem.org/mediawiki/2011/e/ed/2011_10_04_iGEM_SYSU_%E9%9B%86%E4%BD%93%E7%85%A7130.jpg" width="114" height="152" alt="" /><br />
<h1>Garfield ZHAO</h1><br />
<p>Experiemntalist & Wiki Coder &amp; a man's man... &amp; not a professional TOEFLer or GREer...</p><br />
</li><br />
<li><img name="" src="https://static.igem.org/mediawiki/2011/9/9e/2011_10_04_iGEM_SYSU_%E9%9B%86%E4%BD%93%E7%85%A7141.jpg" width="114" height="152" alt="" /><br />
<h1>WenJun SU</h1><br />
<p>Vision Superviser & a little cub ... yes,a little cub indeed...<br />
</li><br />
<br />
</ul><br />
<br />
</div> <br />
</div><br />
</div><br />
<!---------------------------------------------!--><br />
<div id="page_content_RsideBar"><br />
<div class="page_content_relatedBar_aboutus"><br />
<h2>Check our Renren pages:</h2><br />
<h1><a href="http://www.renren.com/profile.do?id=398865894&q=%E7%8E%8B%E5%8A%9B&p=&s=&u=250313171&act=name&rt=user&in=5&ft=2&hh=1">Li Wang's Renren</a></h1><br />
<h1><a href="http://www.renren.com/profile.do?id=268292351&q=%E5%AF%87%E5%BC%BA&p=&s=&u=250313171&act=head&rt=user&in=0&ft=1&hh=1">Qiang Kou's Renren</a></h1><br />
<h1><a href="http://www.renren.com/profile.do?id=243854154&q=%E9%83%AD%E6%B6%9B&p=&s=&u=250313171&act=head&rt=user&in=2&ft=1&hh=1">Tao Guo's Renren</a></h1><br />
<h1><a href="http://www.renren.com/profile.do?id=248684166&q=%E5%AD%99%E5%A8%81%E6%96%87&p=&s=&u=250313171&act=head&rt=user&in=0&ft=1&hh=1">Weiwen Sun's Renren</a></h1><br />
<h1><a href="http://www.renren.com/profile.do?id=265056277&q=%E7%8E%8B%E5%AD%90%E9%BE%99&p=&s=open&u=250313171&act=head&rt=user&in=1&ft=1&hh=1">Zilong Wang's Renren</a></h1><br />
<h1><a href="http://www.renren.com/profile.do?id=248483845&q=%E9%83%91%E6%AF%85&p=&s=&u=250313171&act=head&rt=user&in=0&ft=1&hh=1">Yi Zheng's Renren</a></h1><br />
<h1><a href="http://www.renren.com/profile.do?id=282370064&q=%E5%88%98%E9%A2%96%E6%B4%81&p=&s=&u=250313171&act=head&rt=user&in=0&ft=1&hh=1">Yingjie Liu's Renren</a></h1><br />
<h1><a href="http://www.renren.com/profile.do?id=250313171&from=opensearch">Garfield Zhao's Renren</a></h1><br />
<h1><a href="http://www.renren.com/profile.do?id=256143356&q=%E8%8B%8F%E6%96%87%E9%AA%8F&p=&s=&u=250313171&act=head&rt=user&in=0&ft=1&hh=1">Wenjun Su's RenRen</a></h1><br />
<br /><br />
<p>Note for non-Chinese:</p><br /><br />
<p>&nbsp;&nbsp;&nbsp;&nbsp;Renren is "Chinalized" Facebook. Normally, we can not get access to Facebook or Twitter because of you-know-why....</p><br />
</div><br />
</div> <br />
<!---------------------------------------------!--><br />
<div class="main_page_clearer_light"><br />
</div><br />
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<br />
<!--here begins the contact_bottombar!--><!-- #BeginLibraryItem "/Library/contact_bottombar.lbi" --><div class="contact_bottombar"><br />
<p><a href="https://2011.igem.org/Main_Page">2011 iGEM Mainpage</a></p><br />
<p><a href="https://igem.org/Team_Wikis?year=2011">2011 iGEM Team wikis</a></p><br />
<br />
<p>From the 2011 iGEM team SYSU-China (2011)</p><br />
<p>Sun Yat-Sen University, Guangzhou, China</p><br />
<p>Address: 135# Xingang Rd.(W.), Haizhu Guangzhou, P.R.China</p><br />
<p><a href="http://eng.sysu.edu.cn/">visit the Sun Yat-sen university website</a></p><br />
<p>Thanks Apycom jQuery Menus and <a href="http://apycom.com/">visit their website</a><br />
</div><!-- #EndLibraryItem --><!--here ends the contact_bottombar!--><br />
<br />
</div><!--here ends the backgroud_shadow_bar!--><br />
</body><br />
</html></div>Cambihttp://2011.igem.org/Team:SYSU-China/page_aboutus_team_membersTeam:SYSU-China/page aboutus team members2011-10-28T16:02:36Z<p>Cambi: </p>
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<head><br />
<meta http-equiv="Content-Type" content="text/html; charset=utf-8" /><br />
<title>Team Members-Sun Yat-sen Univ.</title><br />
<link type="text/css" href="menu.css" rel="stylesheet" /><br />
<script type="text/javascript" src="jquery.js"></script><br />
<script type="text/javascript" src="menu.js"></script><br />
<script type="text/javascript" src="scrollTo.js"></script><br />
<link href="style.css" rel="stylesheet" type="text/css" /><br />
<script type="text/javascript" language="javascript"><br />
$(function(){<br />
// $("#page_content_baBar_details").scrollTo(600,2横向)<br />
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//<br />
// <br />
$("#page_content_baBar_details").scrollTo(700)<br />
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<!--------here begins the manu bar section--!--><!-- #BeginLibraryItem "/Library/manu.page.lbi" --><!-- #BeginLibraryItem "/Library/manu.lbi" --><!--------here begins the manu bar section--!--><br />
<div class="title_container"><br />
<div id="menu"><br />
<ul class="menu"><br />
<li><a href="https://2011.igem.org/Team:SYSU-China"><span></span></a></li><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/main_page_news"><span>NEWS</span></a></li><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/main_page_story"><span>STORY</span></a></li> <br />
<li><a href="https://2011.igem.org/Team:SYSU-China/main_page_project" class="parent"><span>PROJECT</span></a><br />
<div><br />
<ul><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/project_bacterial_migration"><span>Bacterial Migration</span></a><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/project_Cesium_Absorption"><span>Cesium Absorption</span></a></li><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/project_Aggregation_Recovery"><span>Aggregation & Recovery</span></a></li><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/page_project_notes"><span>Notes</span></a></li><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/page_project_datapage"><span>Data Page</span></a></li><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/page_project_safety"><span>Safety</span></a></li><br />
</ul><br />
</div><br />
</li><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/main_page_human_practice" class="parent"><span>HUMAN PRACTICE</span></a><br />
<div><br />
<ul><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/page_human_practice_app"><span>iOS App</span></a><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/page_human_practice_labcraft"><span>LabCraft Board Game</span></a></li><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/page_human_practice_workshop"><span>Workshop</span></a></li><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/page_human_practice_survey"><span>Survey</span></a></li><br />
</ul><br />
</div><br />
</li><br />
<br />
<li><a href="https://2011.igem.org/Team:SYSU-China/main_page_aboutus" class="parent"><span>ABOUT US</span></a><br />
<div><br />
<ul><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/page_aboutus_team_members"><span>Team Members</span></a><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/page_aboutus_special_thanks"><span>Special Thanks</span></a></li><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/page_aboutus_LT"><span>Visual Identity</span></a></li><br />
</ul><br />
</div><br />
</li><br />
<br />
</ul><br />
</div><!--here ends the manu!--><br />
<div id="logo_bar"><a href="https://2011.igem.org/Team:SYSU-China"><img src="https://static.igem.org/mediawiki/2011/2/25/Logo_green.png" width="93" height="125" /></a></div><br />
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<div class="page_content_bgBar"><img src="https://static.igem.org/mediawiki/2011/9/9d/Pagehead_tm.jpg" /><br />
<div id="page_content_baBar_details"><a href="#page_content_textBar_enterTEXT">See Details</a></div><br />
</div><br />
<div class="page_content_aboutus_textBar"><br />
<div id="page_content_textBar_enterTEXT"><br />
<br />
<ul><br />
<li><img name="" src="https://static.igem.org/mediawiki/2011/5/5d/2011_10_04_iGEM_SYSU_%E9%9B%86%E4%BD%93%E7%85%A7093.jpg" width="114" height="152" alt="" /><br />
<h1>Li WANG</h1><br />
<p>Western Bloter</p><br />
</li><br />
<li><img name="" src="https://static.igem.org/mediawiki/2011/1/10/2011_10_04_iGEM_SYSU_%E9%9B%86%E4%BD%93%E7%85%A7123.jpg" width="114" height="152" alt="" /><br />
<h1>Qiang KOU</h1><br />
<p>Modeler 模特儿</li><br />
<li><img name="" src="https://static.igem.org/mediawiki/2011/9/9a/2011_10_04_iGEM_SYSU_%E9%9B%86%E4%BD%93%E7%85%A7067.jpg" width="114" height="152" alt="" /><br />
<h1>Tao GUO</h1><br />
<p>iPhone app and wiki coder, the one brings milk... </p><br />
</li><br />
<li><img name="" src="https://static.igem.org/mediawiki/2011/0/00/2011_10_04_iGEM_SYSU_%E9%9B%86%E4%BD%93%E7%85%A7083.jpg" width="114" height="152" alt="" /><br />
<h1>WeiWen SUN</h1><br />
<p>TOEFLer</p><br />
</li><br />
<li><img name="" src="https://static.igem.org/mediawiki/2011/9/93/2011_10_04_iGEM_SYSU_%E9%9B%86%E4%BD%93%E7%85%A7077.jpg" width="114" height="152" alt="" /><br />
<h1>ZiLong WANG</h1><br />
<p>Leader</p><br />
</li><br />
<li><img name="" src="https://static.igem.org/mediawiki/2011/8/80/2011_10_04_iGEM_SYSU_%E9%9B%86%E4%BD%93%E7%85%A7100.jpg" width="114" height="152" alt="" /><br />
<h1>Yi ZHENG</h1><br />
<p>Experiemntalist & Fantastic Dancer</p><br />
<p><br />
</li><br />
<li><img name="" src="https://static.igem.org/mediawiki/2011/7/7c/2011_10_04_iGEM_SYSU_%E9%9B%86%E4%BD%93%E7%85%A7112.jpg" width="114" height="152" alt="" /><br />
<h1>YingJie LIU</h1><br />
<p>Coder, and superisingly, a girl......</p><br />
</li><br />
<li><img name="" src="https://static.igem.org/mediawiki/2011/0/0b/2011_10_04_iGEM_SYSU_%E9%9B%86%E4%BD%93%E7%85%A7082.jpg" width="114" height="152" alt="" /><br />
<h1>YiTian XU</h1><br />
<p>Big fan of Big Bang Theory!! The TV of course!! Experiemntalist ><</p><br />
</li><br />
<li><img name="" src="https://static.igem.org/mediawiki/2011/e/ed/2011_10_04_iGEM_SYSU_%E9%9B%86%E4%BD%93%E7%85%A7130.jpg" width="114" height="152" alt="" /><br />
<h1>Garfield ZHAO</h1><br />
<p>Experiemntalist & Wiki Coder &amp; a man's man... &amp; not a professional TOEFLer or GREer...</p><br />
</li><br />
<li><img name="" src="https://static.igem.org/mediawiki/2011/9/9e/2011_10_04_iGEM_SYSU_%E9%9B%86%E4%BD%93%E7%85%A7141.jpg" width="114" height="152" alt="" /><br />
<h1>WenJun SU</h1><br />
<p>Vision Superviser & a little cub ... yes,a little cub indeed...<br />
</li><br />
<br />
</ul><br />
<br />
</div> <br />
</div><br />
</div><br />
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<div id="page_content_RsideBar"><br />
<div class="page_content_relatedBar_aboutus"><br />
<h2>Check our Renren pages:</h2><br />
<h1><a href="http://www.renren.com/profile.do?id=398865894&q=%E7%8E%8B%E5%8A%9B&p=&s=&u=250313171&act=name&rt=user&in=5&ft=2&hh=1">Li Wang's Renren</a></h1><br />
<h1><a href="http://www.renren.com/profile.do?id=268292351&q=%E5%AF%87%E5%BC%BA&p=&s=&u=250313171&act=head&rt=user&in=0&ft=1&hh=1">Qiang Kou's Renren</a></h1><br />
<h1><a href="http://www.renren.com/profile.do?id=243854154&q=%E9%83%AD%E6%B6%9B&p=&s=&u=250313171&act=head&rt=user&in=2&ft=1&hh=1">Tao Guo's Renren</a></h1><br />
<h1><a href="http://www.renren.com/profile.do?id=248684166&q=%E5%AD%99%E5%A8%81%E6%96%87&p=&s=&u=250313171&act=head&rt=user&in=0&ft=1&hh=1">Weiwen Sun's Renren</a></h1><br />
<h1><a href="http://www.renren.com/profile.do?id=265056277&q=%E7%8E%8B%E5%AD%90%E9%BE%99&p=&s=open&u=250313171&act=head&rt=user&in=1&ft=1&hh=1">Zilong Wang's Renren</a></h1><br />
<h1><a href="http://www.renren.com/profile.do?id=248483845&q=%E9%83%91%E6%AF%85&p=&s=&u=250313171&act=head&rt=user&in=0&ft=1&hh=1">Yi Zheng's Renren</a></h1><br />
<h1><a href="http://www.renren.com/profile.do?id=282370064&q=%E5%88%98%E9%A2%96%E6%B4%81&p=&s=&u=250313171&act=head&rt=user&in=0&ft=1&hh=1">Yingjie Liu's Renren</a></h1><br />
<h1><a href="http://www.renren.com/profile.do?id=250313171&from=opensearch">Garfield Zhao's Renren</a></h1><br />
<h1><a href="http://www.renren.com/profile.do?id=256143356&q=%E8%8B%8F%E6%96%87%E9%AA%8F&p=&s=&u=250313171&act=head&rt=user&in=0&ft=1&hh=1">Wenjun Su's RenRen</a></h1><br />
<br /><br />
<p>Note for non-Chinese:</p><br /><br />
<p>&nbsp;&nbsp;&nbsp;&nbsp;Renren is "Chinalized" Facebook. Normally, we can not get access to Facebook or Twitter because of you-know-why....</p><br />
</div><br />
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<!--here begins the contact_bottombar!--><!-- #BeginLibraryItem "/Library/contact_bottombar.lbi" --><div class="contact_bottombar"><br />
<p><a href="https://2011.igem.org/Main_Page">2011 iGEM Mainpage</a></p><br />
<p><a href="https://igem.org/Team_Wikis?year=2011">2011 iGEM Team wikis</a></p><br />
<br />
<p>From the 2011 iGEM team SYSU-China (2011)</p><br />
<p>Sun Yat-Sen University, Guangzhou, China</p><br />
<p>Address: 135# Xingang Rd.(W.), Haizhu Guangzhou, P.R.China</p><br />
<p><a href="http://eng.sysu.edu.cn/">visit the Sun Yat-sen university website</a></p><br />
<p>Thanks Apycom jQuery Menus and <a href="http://apycom.com/">visit their website</a><br />
</div><!-- #EndLibraryItem --><!--here ends the contact_bottombar!--><br />
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</html></div>Cambihttp://2011.igem.org/Team:SYSU-China/page_human_practice_appTeam:SYSU-China/page human practice app2011-10-27T17:04:12Z<p>Cambi: </p>
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<title>Apple App-Sun Yat-sen Univ.</title><br />
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<div class="title_container"><br />
<div id="menu"><br />
<ul class="menu"><br />
<li><a href="https://2011.igem.org/Team:SYSU-China"><span></span></a></li><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/main_page_news"><span>NEWS</span></a></li><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/main_page_story"><span>STORY</span></a></li> <br />
<li><a href="https://2011.igem.org/Team:SYSU-China/main_page_project" class="parent"><span>PROJECT</span></a><br />
<div><br />
<ul><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/project_bacterial_migration"><span>Bacterial Migration</span></a><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/project_Cesium_Absorption"><span>Cesium Absorption</span></a></li><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/project_Aggregation_Recovery"><span>Aggregation & Recovery</span></a></li><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/page_project_notes"><span>Notes</span></a></li><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/page_project_datapage"><span>Data Page</span></a></li><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/page_project_safety"><span>Safety</span></a></li><br />
</ul><br />
</div><br />
</li><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/main_page_human_practice" class="parent"><span>HUMAN PRACTICE</span></a><br />
<div><br />
<ul><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/page_human_practice_app"><span>iOS App</span></a><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/page_human_practice_labcraft"><span>LabCraft Board Game</span></a></li><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/page_human_practice_workshop"><span>Workshop</span></a></li><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/page_human_practice_survey"><span>Survey</span></a></li><br />
</ul><br />
</div><br />
</li><br />
<br />
<li><a href="https://2011.igem.org/Team:SYSU-China/main_page_aboutus" class="parent"><span>ABOUT US</span></a><br />
<div><br />
<ul><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/page_aboutus_team_members"><span>Team Members</span></a><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/page_aboutus_special_thanks"><span>Special Thanks</span></a></li><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/page_aboutus_LT"><span>Visual Identity</span></a></li><br />
</ul><br />
</div><br />
</li><br />
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</ul><br />
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<div id="logo_bar"><a href="https://2011.igem.org/Team:SYSU-China"><img src="https://static.igem.org/mediawiki/2011/2/25/Logo_green.png" width="93" height="125" /></a></div><br />
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<div class="page_clear_and_introBar"><br />
<img src="https://static.igem.org/mediawiki/2011/4/49/Page_title_bar_app.png" width="958" height="65" /></div><br />
<!------here begins the main content section--!--><br />
<br />
<div id="page_content_WholeBox"><br />
<!---------------------------------------------!--><br />
<div class="page_content_LsideBar"><br />
<div class="page_content_bgBar"><img src="https://static.igem.org/mediawiki/2011/7/79/Pagehead_app.jpg"/><br />
<div id="page_content_baBar_details"><a href="#page_content_textBar_enterTEXT">See Details</a></div><br />
</div><br />
<div class="page_content_textBar"><br />
<div id="page_content_textBar_enterTEXT"><br />
<h2>SynBio Intro</h2><br />
<br /><br />
<p>SynBio Intro is an app runs on iPhone / iPod Touch. It was designed to provide an all-in-one experience of modern biotechnology from explaining "what is DNA" to building an organism on your own. With easy language and friendly UI, you will geta joyful but deep impression on how synthetic biology works and change our lives.</p><br />
<br /><br />
<p>The app is now available for free on App Store.</p> <li><a href="http://itunes.apple.com/us/app/synbio-igem-intro/id468666589?ls=1&mt=8"><span>Visit App Store</span></a></li><br />
<br /><br />
<p>With hundreds of downloads, we've collected some of the comments below:</p><br />
<br /><br />
<p>"The illustrations are fancinating and the introductions are easy to understand. The puzzle is really a challenge so that I have to read the content carefully, think it over, fully understand it and then get the answer. I really learned a lot from the friendly app."</p><br />
<p>-- Knightly, a student in English major</p><br />
<br /><br />
<p>"The app is pretty good. You'll know that as soon as you download it."</p><br />
<p>-- comment from App Store (China)</p><br />
<br /><br />
<p>"Pretty lovely! SynBio is not as difficult as I think!"</p><br />
<p>-- Ngating Wong from Australia</p><br />
<br /><br />
<p>"Could not be better!"</p><br />
<p>-- Maojie Lee, a student in English major</p><br />
</div> <br />
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<link rel="stylesheet" type="text/css" href="Library/jquery.fancybox-1.3.4.css" media="screen" /><br />
<script src="Library/youtube_player.js"></script><br />
<a id="youtube_player" href="http://www.youtube.com/watch?v=Ghxmpo7SKC0&feature=feedwll;feature=player_embedded#at=41"><img src="https://static.igem.org/mediawiki/2011/2/2e/Tour_2.jpg" width="307" height="173" /></a> <!-- #EndLibraryItem --></div><br />
<div class="page_content_relatedBar_humanpractice_app"><br />
<h2>Pages we think helpful:</h2><br />
<h1><a href="http://itunes.apple.com/us/app/synbio-igem-intro/id468666589?ls=1&mt=8">iTune page for SynBio Intro</a></h1><br />
<h1><a href="https://2011.igem.org/Team:SYSU-China/main_page_human_practice">our human practice main page</a></h1><br />
<h1><a href="https://2011.igem.org/Team:SYSU-China/main_page_story">or our Story page</a></h1><br />
<p>&nbsp;</p><br />
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<!--here begins the contact_bottombar!--><!-- #BeginLibraryItem "/Library/contact_bottombar.lbi" --><div class="contact_bottombar"><br />
<p><a href="https://2011.igem.org/Main_Page">2011 iGEM Mainpage</a></p><br />
<p><a href="https://igem.org/Team_Wikis?year=2011">2011 iGEM Team wikis</a></p><br />
<br />
<p>From the 2011 iGEM team SYSU-China (2011)</p><br />
<p>Sun Yat-Sen University, Guangzhou, China</p><br />
<p>Address: 135# Xingang Rd.(W.), Haizhu Guangzhou, P.R.China</p><br />
<p><a href="http://eng.sysu.edu.cn/">visit the Sun Yat-sen university website</a></p><br />
<p>Thanks Apycom jQuery Menus and <a href="http://apycom.com/">visit their website</a><br />
</div><!-- #EndLibraryItem --><!--here ends the contact_bottombar!--><br />
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</html></div>Cambihttp://2011.igem.org/World_Championship_Jamboree/Schedule/Practice_SessionsWorld Championship Jamboree/Schedule/Practice Sessions2011-10-26T04:05:13Z<p>Cambi: </p>
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<img src="https://static.igem.org/mediawiki/2011/4/44/2011_WC_Main_Left1.jpg"><br />
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<!--BEGIN REGONLINE LINK CODE!--><br />
<table style="position: absolute; margin-top:127px; margin-left:15px; z-index: 10;" align="center" class='pbrROL'><tr><td><div class='pbrROL-04' ><div class='ROLbtn'><ul><li><a href='http://www.regonline.com/igem2011worldchampionship' target='_blank' title='iGEM 2011 World Championship Jamboree'><span id='regLink'>Register Now!</span></a></li></ul></div></div></td></tr></table><link rel="stylesheet" href="https://www.regonline.com/styles/ClientButton.css" type="text/css"><br />
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<a href="https://2011.igem.org/World_Championship_Jamboree"><img src="https://static.igem.org/mediawiki/2011/5/55/2011_WC_Main1.jpg"></a><br />
</div><br />
</td><br />
<br />
<td class="menu"><br />
<a href="https://2011.igem.org/World_Championship_Jamboree/Handbook">Jamboree Handbook</a><br />
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<td class="menu"><br />
<a href="https://2011.igem.org/World_Championship_Jamboree/Schedule">Schedule</a><br />
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<a href="https://2011.igem.org/World_Championship_Jamboree/Campus_Map">Campus Map</a><br />
</td><br />
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<a href="https://2011.igem.org/World_Championship_Jamboree/Program">Jamboree Program</a><br />
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<td class="menu"><br />
<a href="https://2011.igem.org/World_Championship_Jamboree/Workshops">Workshops</a><br />
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<td class="menu"><br />
<a href="https://2011.igem.org/World_Championship_Jamboree/Press">Press Kits</a><br />
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<td colspan="3" valign="top"><br />
<div class="main_item"> <!-- World Jamboree Schedule Goes Here--> <br />
<div class="title">Presentation Practice: Friday November 4th 2011</div><br />
<br />
Use this sign-up sheet to sign up for a slot on Friday night (November 4th) to practice your talk. Note that there will NOT be any A/V (audio/visual) support on staff. All classrooms will be unlocked and you should use them and leave them as you found them. <br><br> <br />
<br />
There are a limited number of time slots available on a first-come first-serve basis so please only choose one slot. We cannot match the room that you will ultimately give your presentation in with the practice room. This should, however, give you a chance to practice your talk in a new environment. Please keep in mind that there will be teams waiting to use the room after you, so make sure that your practice finishes on time.<br><br><br />
<br />
Also, on Friday, November 4th, there will also be pre-registration available <!-- beginning at <strong>1pm at Compton Lounge</strong> -->. Conference services will be on-site to pass out team registration boxes (see the <a href="https://2011.igem.org/World_Championship_Jamboree/Handbook">Jamboree Handbook</a>). <br><br><br />
<br />
<strong>Note</strong>: Use the wiki edit button to add your team to the schedule (the markup is located at the bottom of the page). Room numbers and locations will be updated as soon as possible. Additional rooms may be added in the coming weeks.<br />
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<br />
<html> <!--Practice Session Sign-Up Sheet - ADD YOUR TEAM NAME HERE!--><br />
<table class="calendar" align="center"><h2 class="date"><a name="Friday Practice">Friday, November 4</a></h2><br />
<thead><br />
<tr><br />
<th style="width:100px;">Time</th><br />
<th>RM 56-162 </th><br />
<th>RM 34-101 </th><br />
<th>RM 56-154 </th><br />
<th>RM 32-144 </th><br />
<th>RM 10-250 </th><br />
<th>RM 32-123 </th><br />
<th>RM 56-180 </th><br />
<th>RM 56-114 </th><br />
</tr><br />
</thead><br />
<tbody><br />
<tr class="even"><br />
<th>6:00p - 6:30p</th><br />
<td>Washington</td><br />
<td>A2</td><br />
<td>A3</td><br />
<td>A4</td><br />
<td>A5</td><br />
<td>A6</td><br />
<td>A7</td><br />
<td>A8</td><br />
</tr><br />
<tr class="odd"><br />
<th>6:30p - 7:00p</th><br />
<td>B1</td><br />
<td>B2</td><br />
<td>B3</td><br />
<td>B4</td><br />
<td>B5</td><br />
<td>B5</td><br />
<td>B5</td><br />
<td>B5</td><br />
</tr><br />
<tr class="even"><br />
<th>7:00p - 7:30p</th><br />
<td>C1</td><br />
<td>C2</td><br />
<td>C3</td><br />
<td>C4</td><br />
<td>Calgary</td><br />
<td>C6</td><br />
<td>C7</td><br />
<td>C8</td><br />
</tr><br />
<tr class="even"><br />
<th>7:30p - 8:00p</th><br />
<td>D1</td><br />
<td>SYSU-China</td><br />
<td>D3</td><br />
<td>D4</td><br />
<td>D5</td><br />
<td>D6</td><br />
<td>D7</td><br />
<td>D8</td><br />
</tr><br />
<tr class="odd"><br />
<th>8:00p - 8:30p</th><br />
<td>UNICAMP-EMSE</td><br />
<td>E2</td><br />
<td>E3</td><br />
<td>E4</td><br />
<td>E5</td><br />
<td>E6</td><br />
<td>E7</td><br />
<td>E8</td><br />
</tr><br />
<tr class="even"><br />
<th>8:30p - 9:00p</th><br />
<td>F1</td><br />
<td>F2</td><br />
<td>F3</td><br />
<td>F4</td><br />
<td>F5</td><br />
<td>F6</td><br />
<td>F7</td><br />
<td>F8</td><br />
</tr><br />
<tr class="odd"><br />
<th>9:00p - 9:30p</th><br />
<td>G1</td><br />
<td>G2</td><br />
<td>G3</td><br />
<td>G4</td><br />
<td>HKUST-Hong_Kong</td><br />
<td>G6</td><br />
<td>G7</td><br />
<td>G8</td><br />
</tr><br />
<tr class="even"><br />
<th>9:30p - 10:00p</th><br />
<td>Berkeley</td><br />
<td>Queens_Canada</td><br />
<td>UPO-Sevilla</td><br />
<td>Wisconsin-Madison</td><br />
<td>Tokyo-NoKoGen</td><br />
<td>Harvard</td><br />
<td>Johns Hopkins</td><br />
<td>H8</td><br />
</tr><br />
</tbody><br />
</table><br />
</html><br />
<br />
<html><br />
<table><br><br><br />
<center><br />
<iframe width="425" height="350" frameborder="0" scrolling="no" marginheight="0" marginwidth="0" src="http://www.google.com/maps/ms?vpsrc=6&amp;ctz=240&amp;ie=UTF8&amp;msa=0&amp;t=m&amp;msid=208072632249057954752.0004b024a38f4f8325221&amp;source=embed&amp;ll=42.359617,-71.091839&amp;spn=0.000808,0.002135&amp;output=embed"></iframe><br /><small>View <a href="http://www.google.com/maps/ms?vpsrc=6&amp;ctz=240&amp;ie=UTF8&amp;msa=0&amp;t=m&amp;msid=208072632249057954752.0004b024a38f4f8325221&amp;source=embed&amp;ll=42.359617,-71.091839&amp;spn=0.000808,0.002135" style="color:#0000FF;text-align:left">My Saved Places</a> in a larger map</small><br />
</center><br />
</table><br />
</html></div>Cambihttp://2011.igem.org/Team:SYSU-China/page_human_practice_appTeam:SYSU-China/page human practice app2011-10-06T04:04:33Z<p>Cambi: </p>
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<title>Apple App-Sun Yat-sen Univ.</title><br />
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$(function(){<br />
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<!--------here begins the manu bar section--!--><br />
<div class="title_container"><br />
<div id="menu"><br />
<ul class="menu"><br />
<li><a href="index.thml"><span></span></a></li><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/main_page_news"><span>NEWS</span></a></li><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/main_page_story"><span>STORY</span></a></li> <br />
<li><a href="https://2011.igem.org/Team:SYSU-China/main_page_project" class="parent"><span>PROJECT</span></a><br />
<div><br />
<ul><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/page_project_modules_construction"><span>Modules Construction</span></a><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/page_project_modules_verification"><span>Modules Verification</span></a></li><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/page_project_functional_construction"><span>Functional Verification</span></a></li><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/page_project_notes"><span>Notes</span></a></li><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/page_project_datapage"><span>Data Page</span></a></li><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/page_project_safety"><span>Safety</span></a></li><br />
</ul><br />
</div><br />
</li><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/main_page_human_practice" class="parent"><span>HUMAN PRACTICE</span></a><br />
<div><br />
<ul><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/page_human_practice_app"><span>App</span></a><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/page_human_practice_labcraft"><span>LabCraft Board Game</span></a></li><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/page_human_practice_workshop"><span>Workshop</span></a></li><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/page_human_practice_survey"><span>Survey</span></a></li><br />
</ul><br />
</div><br />
</li><br />
<br />
<li><a href="https://2011.igem.org/Team:SYSU-China/main_page_aboutus" class="parent"><span>ABOUT US</span></a><br />
<div><br />
<ul><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/page_aboutus_team_members"><span>Team Members</span></a><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/page_aboutus_special_thanks"><span>Spcial Thanks</span></a></li><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/page_aboutus_LT"><span>Logo and T-shirt</span></a></li><br />
</ul><br />
</div><br />
</li><br />
<br />
</ul><br />
</div><!--here ends the manu!--><br />
</div><!--here ends the title_container!--><br />
<div id="logo_bar"></div> <br />
<!--------here ends the title_section------!--><br />
<br />
<div class="page_clear_and_introBar"><br />
<img src="https://static.igem.org/mediawiki/igem.org/6/6a/Page_human_pratice_apple_app_introBar.png" width="958" height="65" /></div><br />
<!------here begins the main content section--!--><br />
<br />
<div id="page_content_WholeBox"><br />
<!---------------------------------------------!--><br />
<div class="page_content_LsideBar"><br />
<div class="page_content_bgBar"><img src="https://static.igem.org/mediawiki/igem.org/b/b1/Page_human_pratice_apple_app_bg.jpg" width="632" height="600" /><br />
<div id="page_content_baBar_details"><a href="#page_content_textBar_enterTEXT">See Details</a></div><br />
</div><br />
<div class="page_content_textBar"><br />
<div id="page_content_textBar_enterTEXT"><br />
<h2>SynBio Intro</h2><br />
<br /><br />
<p>&nbsp;&nbsp;&nbsp;&nbsp;SynBio Intro is an app runs on iPhone / iPod Touch. It was designed to provide an all-in-one experience of modern biotechnology from explaining &ldquo;what is DNA&rdquo; to building an organism on your own. With easy language and friendly UI, you will get a joyful but deep impression on how synthetic biology works and change our lives.</p><br />
<p>&nbsp;&nbsp;&nbsp;&nbsp;The app is available on App Store. <a href="http://itunes.apple.com/us/app/synbio-igem-intro/id468666589?ls=1&amp;mt=8">Visit App Sotre</a></p><br />
</div> <br />
</div><br />
</div><br />
<!---------------------------------------------!--><br />
<div class="page_content_RsideBar"><br />
<div class="page_content_videoBar"><br />
<br/><br />
<h1>Please watch our animination</h1><br />
<iframe width="307" height="225" src="http://www.youtube.com/embed/KymdcolAhcE?rel=0" frameborder="0" allowfullscreen></iframe><br />
<br />
<br/><br />
<h4>to see the full version of this animation,<a href="http://biogeek.kilu.de/animation.avi<br />
">click here to download</a> </h4><br />
<br/><br />
</div><br />
<div class="page_content_relatedBar_humanpractice_app"><br />
<h2>Pages we think helpful:</h2><br />
<h1><a href="http://itunes.apple.com/us/app/synbio-igem-intro/id468666589?ls=1&mt=8">iTune page for SynBio Intro</a></h1><br />
<h1><a href="https://2011.igem.org/Team:SYSU-China/main_page_human_practice">our human practice main page</a></h1><br />
<h1><a href="https://2011.igem.org/Team:SYSU-China/main_page_story">or our Story page</a></h1><br />
<p>&nbsp;</p><br />
</div><br />
</div> <br />
<!---------------------------------------------!--><br />
<div class="main_page_clearer_light"></div><br />
<!---------------------------------------------!--> <br />
<br />
</div><!--here ends the page_content_WholeBox!--><br />
<div class="main_page_clearer_light"></div><br />
</div><!--here ends the mainpage_project_WholeContainer!--><br />
<br />
<!--here begins the contact_bottombar!--><br />
<div class="contact_bottombar"><br />
<p>From the 2011 iGEM team SYSU-China (2011)</p><br />
<p>Sun Yat-Sen University, Guangzhou, China</p><br />
<p>Address: 135# Xingang Rd.(W.), Haizhu Guangzhou, P.R.China</p><br />
<p><a href="http://eng.sysu.edu.cn/">visit the Sun Yat-sen university website</a></p><br />
<p>Thanks Apycom jQuery Menus and <a href="http://apycom.com/">visit their website</a><br />
</div><br />
<!--here ends the contact_bottombar!--><br />
<br />
</div><!--here ends the backgroud_shadow_bar!--><br />
</body><br />
</html></div>Cambihttp://2011.igem.org/Team:SYSU-China/page_human_practice_surveyTeam:SYSU-China/page human practice survey2011-10-06T03:54:32Z<p>Cambi: </p>
<hr />
<div>{{:Team:SYSU-China/header/temp}}<br />
{{:Team:SYSU-China/header/jscss}}<br />
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<br />
<html xmlns="http://www.w3.org/1999/xhtml"><br />
<head><br />
<meta http-equiv="Content-Type" content="text/html; charset=utf-8" /><br />
<title>Survey-Sun Yat-sen Univ.</title><br />
<br />
<!--------template---------------!--><br />
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<link href="style.css" rel="stylesheet" type="text/css" /><br />
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<script type="text/javascript" src="jquery.js"></script><br />
<script type="text/javascript" src="menu.js"></script><br />
<script type="text/javascript" src="scrollTo.js"></script><br />
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<script type="text/javascript" language="javascript"><br />
$(function(){<br />
// $("#page_content_baBar_details").scrollTo(600,2横向)<br />
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//<br />
// <br />
$("#page_content_baBar_details").scrollTo(700)<br />
});</script><br />
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</head><br />
<br />
<body><br />
<div id="background_shadow"><br />
<div class="page_WholeContainer"> <br />
<br />
<!--------here begins the manu bar section--!--><br />
<div class="title_container"><br />
<div id="menu"><br />
<ul class="menu"><br />
<li><a href="index.thml"><span></span></a></li><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/main_page_news"><span>NEWS</span></a></li><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/main_page_story"><span>STORY</span></a></li> <br />
<li><a href="https://2011.igem.org/Team:SYSU-China/main_page_project" class="parent"><span>PROJECT</span></a><br />
<div><br />
<ul><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/page_project_modules_construction"><span>Modules Construction</span></a><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/page_project_modules_verification"><span>Modules Verification</span></a></li><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/page_project_functional_construction"><span>Functional Verification</span></a></li><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/page_project_notes"><span>Notes</span></a></li><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/page_project_datapage"><span>Data Page</span></a></li><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/page_project_safety"><span>Safety</span></a></li><br />
</ul><br />
</div><br />
</li><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/main_page_human_practice" class="parent"><span>HUMAN PRACTICE</span></a><br />
<div><br />
<ul><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/page_human_practice_app"><span>App</span></a><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/page_human_practice_labcraft"><span>LabCraft Board Game</span></a></li><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/page_human_practice_workshop"><span>Workshop</span></a></li><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/page_human_practice_survey"><span>Survey</span></a></li><br />
</ul><br />
</div><br />
</li><br />
<br />
<li><a href="https://2011.igem.org/Team:SYSU-China/main_page_aboutus" class="parent"><span>ABOUT US</span></a><br />
<div><br />
<ul><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/page_aboutus_team_members"><span>Team Members</span></a><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/page_aboutus_special_thanks"><span>Spcial Thanks</span></a></li><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/page_aboutus_LT"><span>Logo and T-shirt</span></a></li><br />
</ul><br />
</div><br />
</li><br />
<br />
</ul><br />
</div><!--here ends the manu!--><br />
</div><!--here ends the title_container!--><br />
<div id="logo_bar"></div> <br />
<!--------here ends the title_section------!--><br />
<br />
<div class="page_clear_and_introBar"><br />
<img src="https://static.igem.org/mediawiki/igem.org/b/ba/Page_human_pratice_survey_introBar.png" width="958" height="65" /></div><br />
<!------here begins the main content section--!--><br />
<br />
<div id="page_content_WholeBox"><br />
<!---------------------------------------------!--><br />
<div class="page_content_LsideBar"><br />
<div class="page_content_bgBar"><br />
<img src="https://static.igem.org/mediawiki/igem.org/b/b6/Page_human_pratice_survey_bg.jpg" width="632" height="600" /><br />
<div id="page_content_baBar_details"><a href="#page_content_textBar_enterTEXT">See Details</a></div><br />
</div><br />
<div class="page_content_textBar"><br />
<div id="page_content_textBar_enterTEXT"><br />
<!----------text section begins----------------!--> <br />
<br />
<br />
<p>&nbsp;&nbsp;&nbsp;&nbsp;We have conducted a survey to learn about what people think about the synthetic biology. To get more information, we tried to do the survey among people with different background. Most people know something about synthetic biology more or less, and they show some interest in synthetic biology and its applications. The result is shown below. </p><br />
<p>&nbsp;</p><br />
<img src="https://static.igem.org/mediawiki/2011/3/34/Survey1_s.png" width="406" height="537" /><br />
<p>&nbsp;&nbsp;&nbsp;&nbsp;From the two pie charts above, we can see that most people have some knowledge about the synthetic biology and have interests in it. As the words "Synthia" and synthetic biology appear more in the public media, it becomes a hot topic, especially on the issue of bioethics, so we set a question about that.</p><br />
<p>&nbsp;</p><br />
<img src="https://static.igem.org/mediawiki/2011/4/44/Survey_pic2.png" width="406" height="301" /><br />
<p>&nbsp;&nbsp;&nbsp;&nbsp;Most people show some worries about the biosafety, but still think synbio is a promising technique. So we should pay more attention to safety and ethics, while doing experiments.</p><br />
<p>&nbsp;</p><br />
<img src="https://static.igem.org/mediawiki/2011/b/b6/Survey_pic3.png" width="406" height="525" /><br />
<p>&nbsp;&nbsp;&nbsp;&nbsp;As we all know, interdisciplinary study get more attention recently, and synthetic biology is typical in this term. As the result shows, more people think interdisciplinary study will become more important, and more and more people have the idea that other disciplines will contribute to the synthetic biology.</p><br />
<p>&nbsp;</p><br />
<img src="https://static.igem.org/mediawiki/2011/e/eb/Survey_pic4.png" width="406" height="258" /><br />
<p>&nbsp;&nbsp;&nbsp;&nbsp;In terms of the ultimate object of synthetic biology, most people give their vote to the application, and in our opinion, the application is also the ultimate object of iGEM.</p><br />
<br />
<br />
<!-----------text section ends--------------!--> <br />
<br />
<br />
<div class="main_page_clearer_light"></div><br />
</div> <br />
</div><br />
</div><br />
<!---------------------------------------------!--><br />
<div class="page_content_RsideBar"><br />
<div class="page_content_videoBar"><br />
<br/><br />
<h1>Please watch our animination</h1><br />
<iframe width="307" height="225" src="http://www.youtube.com/embed/KymdcolAhcE?rel=0" frameborder="0" allowfullscreen></iframe><br />
<br />
<br/><br />
<h4>to see the full version of this animation,<a href="http://biogeek.kilu.de/animation.avi<br />
">click here to download</a> </h4><br />
<br/><br />
</div><br />
<div class="page_content_relatedBar_humanpractice_survey"><br />
<h2>Pages we think helpful:</h2><br />
<h1><a href="https://2011.igem.org/Team:SYSU-China/page_human_practice_labcraft">check out LabCraft board game</a></h1><br />
<h1><a href="http://biogeek.kilu.de/Lab_Craft_Guide_Book.pdf">Download our LabCraft Guide Book</a></h1><br />
<br />
<h1><a href="http://itunes.apple.com/us/app/synbio-igem-intro/id468666589?ls=1&mt=8">iTune page for SynBio Intro</a></h1><br />
<h1><a href="https://2011.igem.org/Team:SYSU-China/main_page_human_practice">our human practice main page</a></h1><br />
<h1><a href="https://2011.igem.org/Team:SYSU-China/main_page_story">or our Story page</a></h1><br />
</div><br />
</div> <br />
<!---------------------------------------------!--><br />
<div class="main_page_clearer_light"></div><br />
<!---------------------------------------------!--> <br />
<br />
</div><!--here ends the page_content_WholeBox!--><br />
<div class="main_page_clearer_light"></div><br />
</div><!--here ends the mainpage_project_WholeContainer!--><br />
<br />
<!--here begins the contact_bottombar!--><br />
<div class="contact_bottombar"><br />
<p>From the 2011 iGEM team SYSU-China (2011)</p><br />
<p>Sun Yat-Sen University, Guangzhou, China</p><br />
<p>Address: 135# Xingang Rd.(W.), Haizhu Guangzhou, P.R.China</p><br />
<p><a href="http://eng.sysu.edu.cn/">visit the Sun Yat-sen university website</a></p><br />
<p>Thanks Apycom jQuery Menus and <a href="http://apycom.com/">visit their website</a><br />
</div><br />
<!--here ends the contact_bottombar!--><br />
<br />
</div><!--here ends the backgroud_shadow_bar!--><br />
</body><br />
</html></div>Cambihttp://2011.igem.org/Team:SYSU-China/page_human_practice_workshopTeam:SYSU-China/page human practice workshop2011-10-06T03:54:22Z<p>Cambi: </p>
<hr />
<div>{{:Team:SYSU-China/header/temp}}<br />
{{:Team:SYSU-China/header/jscss}}<br />
<br />
<br />
<br />
<html xmlns="http://www.w3.org/1999/xhtml"><br />
<head><br />
<meta http-equiv="Content-Type" content="text/html; charset=utf-8" /><br />
<title>Workshop-Sun Yat-sen Univ.</title><br />
<br />
<!--------template---------------!--><br />
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<link href="style.css" rel="stylesheet" type="text/css" /><br />
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<script type="text/javascript" src="scrollTo.js"></script><br />
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<script type="text/javascript" language="javascript"><br />
$(function(){<br />
// $("#page_content_baBar_details").scrollTo(600,2横向)<br />
<br />
//<br />
// <br />
$("#page_content_baBar_details").scrollTo(700)<br />
});</script><br />
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<style type="text/css"><br />
/* BeginOAWidget_Instance_2127022: #gallery */<br />
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.lbGallery { <br />
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background-color: #ffffff;<br />
padding-left: 0px; <br />
padding-top: 0px; <br />
padding-right: 0px; <br />
padding-bottom: 0px; <br />
width: 180px;<br />
height: auto;<br />
text-align:center;<br />
}<br />
.lbGallery ul { list-style: none; margin:0;padding:0; }<br />
.lbGallery ul li { display: inline;margin:0;padding:0; }<br />
.lbGallery ul li a{text-decoration:none;}<br />
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.lbGallery ul li a img {<br />
/*border color, width and margin for the images*/<br />
border-color: #ffffff;<br />
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<li><a href="index.thml"><span></span></a></li><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/main_page_news"><span>NEWS</span></a></li><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/main_page_story"><span>STORY</span></a></li> <br />
<li><a href="https://2011.igem.org/Team:SYSU-China/main_page_project" class="parent"><span>PROJECT</span></a><br />
<div><br />
<ul><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/page_project_modules_construction"><span>Modules Construction</span></a><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/page_project_modules_verification"><span>Modules Verification</span></a></li><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/page_project_functional_construction"><span>Functional Verification</span></a></li><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/page_project_notes"><span>Notes</span></a></li><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/page_project_datapage"><span>Data Page</span></a></li><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/page_project_safety"><span>Safety</span></a></li><br />
</ul><br />
</div><br />
</li><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/main_page_human_practice" class="parent"><span>HUMAN PRACTICE</span></a><br />
<div><br />
<ul><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/page_human_practice_app"><span>App</span></a><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/page_human_practice_labcraft"><span>LabCraft Board Game</span></a></li><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/page_human_practice_workshop"><span>Workshop</span></a></li><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/page_human_practice_survey"><span>Survey</span></a></li><br />
</ul><br />
</div><br />
</li><br />
<br />
<li><a href="https://2011.igem.org/Team:SYSU-China/main_page_aboutus" class="parent"><span>ABOUT US</span></a><br />
<div><br />
<ul><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/page_aboutus_team_members"><span>Team Members</span></a><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/page_aboutus_special_thanks"><span>Spcial Thanks</span></a></li><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/page_aboutus_LT"><span>Logo and T-shirt</span></a></li><br />
</ul><br />
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<br />
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<p>&nbsp;&nbsp;&nbsp;&nbsp;We ran a workshop in our school with junior students. For more effective group discussion, we limit the participants to no more than 15 students. </p><br />
<p>&nbsp;&nbsp;&nbsp;&nbsp;Firstly, we introduced the concept and achievements of synthetic biology. Then we talked about the Biobrick and iGEM. As we think, the workshop is not only for iGEM or synthetic biology, we also spend much time talking about problems they maybe meet in their future study.</p><br />
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<li> <a href="https://static.igem.org/mediawiki/igem.org/2/21/NEO_IMG_IMG_4418.jpg" title=""><img src="https://static.igem.org/mediawiki/igem.org/7/76/NEO_IMG_IMG_4418s.jpg" width="150" height="92" alt="Flower" /></a> </li><br />
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<!---------------------------------------------!--><br />
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<br/><br />
<h1>Please watch our animination</h1><br />
<iframe width="307" height="225" src="http://www.youtube.com/embed/KymdcolAhcE?rel=0" frameborder="0" allowfullscreen></iframe><br />
<br />
<br/><br />
<h4>to see the full version of this animation,<a href="http://biogeek.kilu.de/animation.avi<br />
">click here to download</a> </h4><br />
<br/><br />
<br />
</div><br />
<div class="page_content_relatedBar_humanpractice_workshop"><br />
<h2>Pages we think helpful:</h2><br />
<h1><a href="https://2011.igem.org/Team:SYSU-China/page_human_practice_labcraft">check out LabCraft board game</a></h1><br />
<h1><a href="http://biogeek.kilu.de/Lab_Craft_Guide_Book.pdf">Download our LabCraft Guide Book</a></h1><br />
<br />
<h1><a href="http://itunes.apple.com/us/app/synbio-igem-intro/id468666589?ls=1&mt=8">iTune page for SynBio Intro</a></h1><br />
<h1><a href="https://2011.igem.org/Team:SYSU-China/main_page_human_practice">our human practice main page</a></h1><br />
<h1><a href="https://2011.igem.org/Team:SYSU-China/main_page_story">or our Story page</a></h1><br />
</div><br />
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<p>From the 2011 iGEM team SYSU-China (2011)</p><br />
<p>Sun Yat-Sen University, Guangzhou, China</p><br />
<p>Address: 135# Xingang Rd.(W.), Haizhu Guangzhou, P.R.China</p><br />
<p><a href="http://eng.sysu.edu.cn/">visit the Sun Yat-sen university website</a></p><br />
<p>Thanks Apycom jQuery Menus and <a href="http://apycom.com/">visit their website</a><br />
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</html></div>Cambihttp://2011.igem.org/Team:SYSU-China/page_human_practice_labcraftTeam:SYSU-China/page human practice labcraft2011-10-06T03:53:40Z<p>Cambi: </p>
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<ul class="menu"><br />
<li><a href="index.thml"><span></span></a></li><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/main_page_news"><span>NEWS</span></a></li><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/main_page_story"><span>STORY</span></a></li> <br />
<li><a href="https://2011.igem.org/Team:SYSU-China/main_page_project" class="parent"><span>PROJECT</span></a><br />
<div><br />
<ul><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/page_project_modules_construction"><span>Modules Construction</span></a><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/page_project_modules_verification"><span>Modules Verification</span></a></li><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/page_project_functional_construction"><span>Functional Verification</span></a></li><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/page_project_notes"><span>Notes</span></a></li><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/page_project_datapage"><span>Data Page</span></a></li><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/page_project_safety"><span>Safety</span></a></li><br />
</ul><br />
</div><br />
</li><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/main_page_human_practice" class="parent"><span>HUMAN PRACTICE</span></a><br />
<div><br />
<ul><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/page_human_practice_app"><span>App</span></a><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/page_human_practice_labcraft"><span>LabCraft Board Game</span></a></li><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/page_human_practice_workshop"><span>Workshop</span></a></li><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/page_human_practice_survey"><span>Survey</span></a></li><br />
</ul><br />
</div><br />
</li><br />
<br />
<li><a href="https://2011.igem.org/Team:SYSU-China/main_page_aboutus" class="parent"><span>ABOUT US</span></a><br />
<div><br />
<ul><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/page_aboutus_team_members"><span>Team Members</span></a><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/page_aboutus_special_thanks"><span>Spcial Thanks</span></a></li><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/page_aboutus_LT"><span>Logo and T-shirt</span></a></li><br />
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<div id="page_content_baBar_details"><a href="#page_content_textBar_enterTEXT">See Details</a></div><br />
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<div class="page_content_textBar"><br />
<div id="page_content_textBar_enterTEXT"><br />
<div class="page_content_textBar_enterTEXT_enter"><br />
<h2>Click <a href="http://biogeek.kilu.de/Lab_Craft_Guide_Book.pdf">HERE</a> to download LabCraft Guide Book</h2><br />
<br /><br />
<h2>Welcome to the World of LabCraft</h2><br />
<br /><br />
<p>&nbsp;&nbsp;&nbsp;&nbsp;LabCraft is a shootout game that happens in a biological lab. In the lab, Everything is like having a war.</p><br />
<br />
<p>&nbsp;&nbsp;&nbsp;&nbsp;The game itself focus between a group of Postdocs and the PI, who is their primary target. The PhDs incognito help the PI, but there is also a Master pursuing his own goal! </p><br />
<br />
<p>&nbsp;&nbsp;&nbsp;&nbsp;In LabCraft each player plays one of these roles, and represents a famous lab character.</p> <br />
<br />
<p>&nbsp;&nbsp;&nbsp;&nbsp;Have fun and enjoy yourself with your friends, classmates, colleagues, even your teachers.<br />
<br /><br />
<br /><br />
<h2>CONTENTS</h2><br />
<br /><br />
<p>&nbsp;&nbsp;&nbsp;&nbsp;119 cards of three different types, they can be identified by their back:</p><br />
<br />
<h1>7 "ROLE" cards:</h1> <br />
<p>&nbsp;&nbsp;&nbsp;&nbsp;1 PI, 2 PhDs, 3 Postdocs, 1 Master.</p><br />
<br />
<h1>8 "CHARACTER" cards,</h1> <br />
<p>&nbsp;&nbsp;&nbsp;&nbsp;with colonies printed on the left side;.</p><br />
<br />
<h1>104 playing cards,</h1><br />
<p>&nbsp;&nbsp;&nbsp;&nbsp;also called funtional cards, printed "LabCraft" on their back.</p><br />
<br />
<h1>"colonies" cards</h1><br />
<p>&nbsp;&nbsp;&nbsp;&nbsp;And by the way, in the box we also offered several "colonies" cards printed with three, four or five colonies (circles that represented the blood/life you left).</p><br />
<br /><br />
<br /><br />
<h2>OBJECT</h2><br />
<br /><br />
<p>&nbsp;&nbsp;&nbsp;&nbsp;Each player has his own goal:</p><br />
<br />
<h1>PI</h1><br />
<p>&nbsp;&nbsp;&nbsp;&nbsp;They must eliminate all the Postdocs and the Master, to protect the lab.</p><br />
<br />
<h1>Postdoc</h1><br />
<p>&nbsp;&nbsp;&nbsp;&nbsp;They would like to eliminate the PI, but they have no scruples about eliminating each other to gain rewards!</p><br />
<br />
<h1>PhD</h1><br />
<p>&nbsp;&nbsp;&nbsp;&nbsp;They help and protect the PI, and share his same goal, at all costs!</p><br />
<br />
<h1>Master</h1><br />
<p>&nbsp;&nbsp;&nbsp;&nbsp;He wants to be the new PI; his goal is to be the last character in play.</p><br />
<br /><br />
<br /><br />
<h2>CHARACTERS</h2><br />
<br /><br />
<p>&nbsp;&nbsp;&nbsp;&nbsp;Each lab character has some special abilities, which make him unique. The series of colonies near the character's picture show how many life points the character begins with, i.e. how many times he can be hit before being eliminated from play. </p><br />
<br />
<p>Moreover, the conlonies indicate how many cards the player can hold in his hand at the end of his turn (hand size limit).</p><br />
<br /><br />
<br /><br />
<h2>THE GAME</h2><br />
<br /><br />
<p>&nbsp;&nbsp;&nbsp;&nbsp;The game is played in turns, in clockwise order, with a beginning from PI. Each player's turn is divided into three phases:</p><br />
<br />
<h1>1. Draw two cards;</h1><br />
<h1>2. Play any number of cards;</h1><br />
<h1>3. Discard excess cards.</h1><br />
<br />
<br />
<li>&nbsp;&nbsp;&nbsp;&nbsp;1. Draw two cards</li><br />
<p>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;The active player draws the top two cards from the draw pile. As soon as the draw pile is empty, shuffle the discard pile to create a new playing deck.</p><br />
<br />
<li>&nbsp;&nbsp;&nbsp;&nbsp;2. Play any number of cards</li><br />
<p>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Now the player may play cards to help himself or hurt the other players, trying to eliminate them. He is not forced to play cards during this phase. Any number of cards may be played.</p><br />
<br />
<p>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;There are only two limitations:</p><br />
<li>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;1) Only one Antibiotic! card may be played per turn;</li><br />
<li>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;2) No player can ever have two identical cards face up in front of him.</li><br />
<br />
<p>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;When a card is played, just follow the instruction on it. Cards can be played only during your turn (with the exception of "Medium" and "Biofilm!" cards).</p><br />
<br />
<p>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Normally a card has an effect which is immediately resolved, and then the card is discarded. However, "Equipments" cards have long-lasting effects, and are kept on the table face up in front of you. The effect of these cards ("in play" cards) lasts until they are discarded or removed somehow, or a special event occurs .</p><br />
<br />
<li>&nbsp;&nbsp;&nbsp;&nbsp;3. Discard excess cards</li><br />
<p>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Once the second phase is over (you do not want to or cannot play any more cards), then you must discard from your hand any cards exceeding your hand-size limit. Remember that the hand size limit of a player, at the end of his turn, is equal to the number of life points he left. </p><br />
<br />
<p>Then it is the next player's turn, in clockwise order.</p><br />
<br /><br /><br />
<p>&nbsp;&nbsp;&nbsp;&nbsp;Please download the Guide Book of LabCraft (PDF) to learn more about our LabCraft.</p><br />
<br />
</div><br />
<br />
<div class="page_content_text_Bar_enterTEXT_RpicBar"><br />
<div id="gallery" class="lbGallery"><br />
<ul><br />
<li> <a href="https://static.igem.org/mediawiki/igem.org/5/5d/Figure_1_update.jpg" title=""><img src="https://static.igem.org/mediawiki/igem.org/9/91/Figure_1_s.jpg" /></a> </li><br />
<h2>Figure 1.</h2>The box art (front cover) of the board game.<br />
<li> <a href="https://static.igem.org/mediawiki/igem.org/8/80/Figure_2.jpg" title=""><img src="https://static.igem.org/mediawiki/igem.org/0/09/Figure_2_s.jpg" /></a> </li><br />
<h2>Figure 2.</h2>The "Character" cards.<br />
<li> <a href="https://static.igem.org/mediawiki/igem.org/c/c9/Figure_3.jpg" title=""><img src="https://static.igem.org/mediawiki/igem.org/2/29/Figure_3_s.jpg" /></a> </li><br />
<h2>Figure 3.</h2>The three basic game playing (or functional) cards.<br />
</ul><br />
</div><br />
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</div><br />
<!---------------------------------------------!--><br />
<div class="page_content_RsideBar"><br />
<div class="page_content_videoBar"><br />
<br/><br />
<h1>Please watch our animination</h1><br />
<iframe width="307" height="225" src="http://www.youtube.com/embed/KymdcolAhcE?rel=0" frameborder="0" allowfullscreen></iframe><br />
<br />
<br/><br />
<h4>to see the full version of this animation,<a href="http://biogeek.kilu.de/animation.avi<br />
">click here to download</a> </h4><br />
<br/><br />
<br />
</div><br />
<div class="page_content_relatedBar_humanpractice_LB"><br />
<h2>Pages we think helpful:</h2><br />
<h1><a href="http://biogeek.kilu.de/Lab_Craft_Guide_Book.pdf">Download our LabCraft Guide Book</a></h1><br />
<br />
<h1><a href="http://itunes.apple.com/us/app/synbio-igem-intro/id468666589?ls=1&mt=8">iTune page for SynBio Intro</a></h1><br />
<h1><a href="https://2011.igem.org/Team:SYSU-China/main_page_human_practice">our human practice main page</a></h1><br />
<h1><a href="https://2011.igem.org/Team:SYSU-China/main_page_story">or our Story page</a></h1><br />
<p>&nbsp;</p><br />
</div><br />
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<!--here begins the contact_bottombar!--><br />
<div class="contact_bottombar"><br />
<p>From the 2011 iGEM team SYSU-China (2011)</p><br />
<p>Sun Yat-Sen University, Guangzhou, China</p><br />
<p>Address: 135# Xingang Rd.(W.), Haizhu Guangzhou, P.R.China</p><br />
<p><a href="http://eng.sysu.edu.cn/">visit the Sun Yat-sen university website</a></p><br />
<p>Thanks Apycom jQuery Menus and <a href="http://apycom.com/">visit their website</a><br />
</div><br />
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</html></div>Cambihttp://2011.igem.org/Team:SYSU-China/page_human_practice_appTeam:SYSU-China/page human practice app2011-10-06T03:53:27Z<p>Cambi: </p>
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<ul class="menu"><br />
<li><a href="index.thml"><span></span></a></li><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/main_page_news"><span>NEWS</span></a></li><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/main_page_story"><span>STORY</span></a></li> <br />
<li><a href="https://2011.igem.org/Team:SYSU-China/main_page_project" class="parent"><span>PROJECT</span></a><br />
<div><br />
<ul><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/page_project_modules_construction"><span>Modules Construction</span></a><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/page_project_modules_verification"><span>Modules Verification</span></a></li><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/page_project_functional_construction"><span>Functional Verification</span></a></li><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/page_project_notes"><span>Notes</span></a></li><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/page_project_datapage"><span>Data Page</span></a></li><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/page_project_safety"><span>Safety</span></a></li><br />
</ul><br />
</div><br />
</li><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/main_page_human_practice" class="parent"><span>HUMAN PRACTICE</span></a><br />
<div><br />
<ul><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/page_human_practice_app"><span>App</span></a><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/page_human_practice_labcraft"><span>LabCraft Board Game</span></a></li><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/page_human_practice_workshop"><span>Workshop</span></a></li><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/page_human_practice_survey"><span>Survey</span></a></li><br />
</ul><br />
</div><br />
</li><br />
<br />
<li><a href="https://2011.igem.org/Team:SYSU-China/main_page_aboutus" class="parent"><span>ABOUT US</span></a><br />
<div><br />
<ul><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/page_aboutus_team_members"><span>Team Members</span></a><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/page_aboutus_special_thanks"><span>Spcial Thanks</span></a></li><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/page_aboutus_LT"><span>Logo and T-shirt</span></a></li><br />
</ul><br />
</div><br />
</li><br />
<br />
</ul><br />
</div><!--here ends the manu!--><br />
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<br />
<div class="page_clear_and_introBar"><br />
<img src="https://static.igem.org/mediawiki/igem.org/6/6a/Page_human_pratice_apple_app_introBar.png" width="958" height="65" /></div><br />
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<div id="page_content_baBar_details"><a href="#page_content_textBar_enterTEXT">See Details</a></div><br />
</div><br />
<div class="page_content_textBar"><br />
<div id="page_content_textBar_enterTEXT"><br />
<h2>SynBio Intro</h2><br />
<br /><br />
<p>&nbsp;&nbsp;&nbsp;&nbsp;SynBio Intro is an app runs on iPhone / iPod Touch. It was designed to provide an all-in-one experience of modern biotechnology from explaining &ldquo;what is DNA&rdquo; to building an organism on your own. With easy language and friendly UI, you will get a joyful but deep impression on how synthetic biology works and change our lives.</p><br />
<p>&nbsp;&nbsp;&nbsp;&nbsp;The app is under review and will be available soon on <a href="http://itunes.apple.com/us/app/synbio-igem-intro/id468666589?ls=1&amp;mt=8">http://itunes.apple.com/us/app/synbio-igem-intro/id468666589?ls=1&amp;mt=8</a></p><br />
</div> <br />
</div><br />
</div><br />
<!---------------------------------------------!--><br />
<div class="page_content_RsideBar"><br />
<div class="page_content_videoBar"><br />
<br/><br />
<h1>Please watch our animination</h1><br />
<iframe width="307" height="225" src="http://www.youtube.com/embed/KymdcolAhcE?rel=0" frameborder="0" allowfullscreen></iframe><br />
<br />
<br/><br />
<h4>to see the full version of this animation,<a href="http://biogeek.kilu.de/animation.avi<br />
">click here to download</a> </h4><br />
<br/><br />
</div><br />
<div class="page_content_relatedBar_humanpractice_app"><br />
<h2>Pages we think helpful:</h2><br />
<h1><a href="http://itunes.apple.com/us/app/synbio-igem-intro/id468666589?ls=1&mt=8">iTune page for SynBio Intro</a></h1><br />
<h1><a href="https://2011.igem.org/Team:SYSU-China/main_page_human_practice">our human practice main page</a></h1><br />
<h1><a href="https://2011.igem.org/Team:SYSU-China/main_page_story">or our Story page</a></h1><br />
<p>&nbsp;</p><br />
</div><br />
</div> <br />
<!---------------------------------------------!--><br />
<div class="main_page_clearer_light"></div><br />
<!---------------------------------------------!--> <br />
<br />
</div><!--here ends the page_content_WholeBox!--><br />
<div class="main_page_clearer_light"></div><br />
</div><!--here ends the mainpage_project_WholeContainer!--><br />
<br />
<!--here begins the contact_bottombar!--><br />
<div class="contact_bottombar"><br />
<p>From the 2011 iGEM team SYSU-China (2011)</p><br />
<p>Sun Yat-Sen University, Guangzhou, China</p><br />
<p>Address: 135# Xingang Rd.(W.), Haizhu Guangzhou, P.R.China</p><br />
<p><a href="http://eng.sysu.edu.cn/">visit the Sun Yat-sen university website</a></p><br />
<p>Thanks Apycom jQuery Menus and <a href="http://apycom.com/">visit their website</a><br />
</div><br />
<!--here ends the contact_bottombar!--><br />
<br />
</div><!--here ends the backgroud_shadow_bar!--><br />
</body><br />
</html></div>Cambihttp://2011.igem.org/Team:SYSU-China/page_project_safetyTeam:SYSU-China/page project safety2011-10-06T03:53:09Z<p>Cambi: </p>
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<script type="text/javascript" src="jquery.js"></script><br />
<script type="text/javascript" src="menu.js"></script><br />
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<div class="title_container"><br />
<div id="menu"><br />
<ul class="menu"><br />
<li><a href="index.thml"><span></span></a></li><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/main_page_news"><span>NEWS</span></a></li><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/main_page_story"><span>STORY</span></a></li> <br />
<li><a href="https://2011.igem.org/Team:SYSU-China/main_page_project" class="parent"><span>PROJECT</span></a><br />
<div><br />
<ul><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/page_project_modules_construction"><span>Modules Construction</span></a><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/page_project_modules_verification"><span>Modules Verification</span></a></li><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/page_project_functional_construction"><span>Functional Verification</span></a></li><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/page_project_notes"><span>Notes</span></a></li><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/page_project_datapage"><span>Data Page</span></a></li><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/page_project_safety"><span>Safety</span></a></li><br />
</ul><br />
</div><br />
</li><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/main_page_human_practice" class="parent"><span>HUMAN PRACTICE</span></a><br />
<div><br />
<ul><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/page_human_practice_app"><span>App</span></a><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/page_human_practice_labcraft"><span>LabCraft Board Game</span></a></li><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/page_human_practice_workshop"><span>Workshop</span></a></li><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/page_human_practice_survey"><span>Survey</span></a></li><br />
</ul><br />
</div><br />
</li><br />
<br />
<li><a href="https://2011.igem.org/Team:SYSU-China/main_page_aboutus" class="parent"><span>ABOUT US</span></a><br />
<div><br />
<ul><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/page_aboutus_team_members"><span>Team Members</span></a><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/page_aboutus_special_thanks"><span>Spcial Thanks</span></a></li><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/page_aboutus_LT"><span>Logo and T-shirt</span></a></li><br />
</ul><br />
</div><br />
</li><br />
<br />
</ul><br />
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<br />
<h2>SAFETY AND SECURITY QUESTIONS</h2><br />
<p>&nbsp;</p><br />
<h1>1. Would the materials used in your project and/or your final product pose: </h1><br />
<p>a. Risks to the safety and health of team members or others in the lab? </P><br />
<p>b. Risks to the safety and health of the general public if released by design or accident? </P><br />
<p>c. Risks to environmental quality if released by design or accident? </P><br />
<p>d. Risks to security through malicious misuse by individuals, groups or states? </P><br />
<p>Please explain your responses (whether yes or no) to these questions. </P><br />
<p>Specifically, are any parts or devices in your project associated with (or known to cause): </P> <br />
<p>- pathogenicity, infectivity, or toxicity? </P> <br />
<p>- threats to environmental quality? </P><br />
<p>- security concerns? </P> <br /><br />
<br />
<p>&nbsp;&nbsp;&nbsp;&nbsp;From the aspect of the project itself, no materials used by the iGEM 2011 SYSU-China or any final product would raise any safety issue in terms of researchers, the public or the environment. </P> <br /><br />
<p>&nbsp;&nbsp;&nbsp;&nbsp;First, the bacteria we are working on are Escherichia coli BL21 and DH5α,the non-virulent mutants of Escherichia coli, which are considered to be non-pathogenic and unlikely to survive in host tissues and cause disease. These are confirmed by R.M. La Ragione and M.J. Woodwad in their paper An investigation into the pathogenic properties of Escherichia coli strains BLR, BL21, DH5a and EQ1 (http://ors.uchc.edu/bio/resources/pdf/3.6.1.A_colipath.pdf). </P> <br /><br />
<p>&nbsp;&nbsp;&nbsp;&nbsp;Second, since several years' operation of the two kinds of E.coli in the labs and no virulence against human has been reported, we believe that these strains are harmless to team members or others in the lab. Except to that, team members or others in the lab are protected appropriately, such as wearing gloves and masks, and this also reduces the risks that they are infected or get other hurts in the lab. </P> <br /><br />
<p>&nbsp;&nbsp;&nbsp;&nbsp;Third, even if our bacteria are released from the lab by design or accident, they will not pose any risk to the safety and health of the general public, because as we mentioned above, they are the non-virulent mutants of Escherichia coli, and are non-pathogenic and unlikely to survive in host tissues and cause disease. As a result, they are safe to the general public. In terms of the environment, our bacteria are difficult to survive in the natural environment, because they need enough nutrition in which the compositions are in accordance with an appropriate proportion. Since they are difficult to survive, they are unlikely to cause safety problems to the environment. As for the malicious misuse of these bacteria, it is difficult to get the guarantee that they are doomed to be safe. Basically, as long as they are not transformed maliciously, they will not be able to cause the safety issues even if they are maliciously misused. </P> <br /><br />
<br />
<h1>2. Do any of the new BioBrick parts (or devices) that you made this year raise any safety issues? If yes, </h1><br />
<p>a. Did you document these issues in the Registry? </P><br />
<p>b. How did you manage to handle the safety issue? </P><br />
<p>c. How could other teams learn from your experience? </P><br /><br />
<br />
<p>&nbsp;&nbsp;&nbsp;&nbsp;No biobricks made by the SYSU-China team will raise any safety issues. The biobricks, such as recA, recN, cheZ, trkD and ag43, are all the natural parts of E.coli in natural environment, and commonly used for laboratory operation. Their function and functional mechanism are clearly known, and they are not associated with pathogenicity, infectivity, or toxicity, nor will they cause threats to environmental quality. Moreover, cheZ and ag43 are standard biobricks provided by iGEM authority, so the safety of these biobricks can be guaranteed. </P> <br /><br />
<br />
<h1>3. Under what biosafety provisions will / do you operate? </h1><br />
<p>a. Does your institution have its own biosafety rules and if so what are they? Provide a link to them online if possible. </P><br />
<p>b. Does your institution have an Institutional Biosafety Committee or equivalent group? If yes, have you discussed your project with them? Describe any concerns or changes that were made based on this review. </P><br />
<p>c. Will / did you receive any biosafety and/or lab training before beginning your project? If so, describe this training. </P><br />
<p>d. Does your country have national biosafety regulations or guidelines? If so, provide a link to them online if possible. </P> <br /><br />
<br />
<p>&nbsp;&nbsp;&nbsp;&nbsp;First, the SYSU_China team members conform strictly to the established safety rules in the lab (http://biosafety.sysu.edu.cn/administer/UploadFiles_9471/200804/2008041817410158.pdf). </P> <br /><br />
<p>&nbsp;&nbsp;&nbsp;&nbsp;Second, In our university, Biosafety Committee of Sun Yat-sen University is responsible for monitoring the safety of all the research activities on the campus. Their regulations are stipulated according to the WHO Laboratory biosafety manual, which is also conformed to by our country (http://biosafety.sysu.edu.cn/Soft/UploadSoft/200804/2008042116071397.pdf). Before our team started the program, we have talked about the safety issues of the whole project. They were really concerned about the radiation-related experiments, and emphasized that these experiments must be operated by the professional technician. In addition to that, they also requested that all procedures conducted in this project should be performed according to the rules stipulated by WHO. On the whole, they think about our project as safe. With the surveillance of the Biosafety Committee of Sun Yat-sen University and the cautious operation of our members, the project will be harmless to the researchers, the public or the environment. </P> <br /><br />
<p>&nbsp;&nbsp;&nbsp;&nbsp;Third, each team member was required to attend a pre-lab training led by graduate students and advisors both on experimental skills and safety instructions before he or she actually started to do the iGEM program in the lab. They trained us how to operate the fundamental experiments, and taught us the basic safety rules in the lab, such as how to use the toxic reagents. During the program, we wear gloves properly and disinfect tubes and plates after use. When it involves the radiation-related experiments, the operation will be carried out by the technician with radiation-usage permission. </P> <br /><br />
<br />
<br />
<h1>4. OPTIONAL QUESTION: Do you have other ideas on how to deal with safety or security issues that could be useful for future iGEM competitions? How could parts, devices and systems be made even safer through biosafety engineering? </h1> <br /><br />
<br />
<p>&nbsp;&nbsp;&nbsp;&nbsp;We suggest that the safety information of any part used in the iGEM competition, especially its functions, should be investigated clearly through literature search before the usage. If the part has any intention to raise safety issues, the iGEM team should refuse to use it and choose other alternatives. In addition to that, iGEM teams should submit the safety report with their biobricks part to the iGEM authority, describing the safety issues of the biobricks part, which should also be examined by a professional third party. Thus the high-risk biobricks or parts with potential biosafety problems will be identified and abandoned before submitting. Through this way can the parts, devices and systems be made much safer. With above measures, the safety of the biobricks submitted can be guaranteed. </P> <br /><br />
<br />
<br />
<br />
</div> <br />
</div><br />
</div><br />
<!---------------------------------------------!--><br />
<div class="page_content_RsideBar"><br />
<div class="page_content_videoBar"><br />
<br/><br />
<h1>Please watch our animination</h1><br />
<iframe width="307" height="225" src="http://www.youtube.com/embed/KymdcolAhcE?rel=0" frameborder="0" allowfullscreen></iframe><br />
<br />
<br/><br />
<h4>to see the full version of this animation,<a href="http://biogeek.kilu.de/animation.avi<br />
">click here to download</a> </h4><br />
<br/><br />
<br />
</div><br />
<div class="page_content_relatedBar_project_sf"><br />
<h2>Pages we think helpful:</h2><br />
<br />
<h1><a href="https://2011.igem.org/Team:SYSU-China/page_project_modules_construction">see how we construct our modules</a></h1><br />
<h1><a href="https://2011.igem.org/Team:SYSU-China/page_project_modules_verification">see how we verify each module</a></h1><br />
<h1><a href="https://2011.igem.org/Team:SYSU-China/main_page_story">or see our whole idea in Story page</a></h1><br />
<p>&nbsp;</p><br />
</div><br />
</div> <br />
<!---------------------------------------------!--><br />
<div class="main_page_clearer_light"></div><br />
<!---------------------------------------------!--> <br />
<br />
</div><!--here ends the page_content_WholeBox!--><br />
<div class="main_page_clearer_light"></div><br />
</div><!--here ends the mainpage_project_WholeContainer!--><br />
<br />
<!--here begins the contact_bottombar!--><br />
<div class="contact_bottombar"><br />
<p>From the 2011 iGEM team SYSU-China (2011)</p><br />
<p>Sun Yat-Sen University, Guangzhou, China</p><br />
<p>Address: 135# Xingang Rd.(W.), Haizhu Guangzhou, P.R.China</p><br />
<p><a href="http://eng.sysu.edu.cn/">visit the Sun Yat-sen university website</a></p><br />
<p>Thanks Apycom jQuery Menus and <a href="http://apycom.com/">visit their website</a><br />
</div><br />
<!--here ends the contact_bottombar!--><br />
<br />
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</body><br />
</html></div>Cambihttp://2011.igem.org/Team:SYSU-China/page_project_datapageTeam:SYSU-China/page project datapage2011-10-06T03:52:14Z<p>Cambi: </p>
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<li><a href="https://2011.igem.org/Team:SYSU-China/main_page_story"><span>STORY</span></a></li> <br />
<li><a href="https://2011.igem.org/Team:SYSU-China/main_page_project" class="parent"><span>PROJECT</span></a><br />
<div><br />
<ul><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/page_project_modules_construction"><span>Modules Construction</span></a><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/page_project_modules_verification"><span>Modules Verification</span></a></li><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/page_project_functional_construction"><span>Functional Verification</span></a></li><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/page_project_notes"><span>Notes</span></a></li><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/page_project_datapage"><span>Data Page</span></a></li><br />
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</ul><br />
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<li><a href="https://2011.igem.org/Team:SYSU-China/main_page_human_practice" class="parent"><span>HUMAN PRACTICE</span></a><br />
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<li><a href="https://2011.igem.org/Team:SYSU-China/page_human_practice_app"><span>App</span></a><br />
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<img src="https://static.igem.org/mediawiki/igem.org/5/57/SYSU_Page_project_data_page_introBar.png<br />
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<!------here begins the main content section--!--><br />
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<div id="page_content_WholeBox"><br />
<!---------------------------------------------!--><br />
<div class="page_content_LsideBar"><br />
<div class="page_content_bgBar"><img src="https://static.igem.org/mediawiki/igem.org/0/0d/SYSU_Page_project_data_page_bg.jpg<br />
" width="632" height="600" /><br />
<div id="page_content_baBar_details"><a href="#page_content_textBar_enterTEXT">See Details</a></div><br />
</div><br />
<div class="page_content_textBar"><br />
<div id="page_content_textBar_enterTEXT"><br />
<br />
<img src="https://static.igem.org/mediawiki/2011/7/7f/Story_Figure_2.jpg" width="386" height="161" /><br />
<p>This is the Che protein family system we had used<br />
(Picture is from Shana Topp and Justin P. Gallivan, J. AM. CHEM. SOC. 2007, 129, 6807 6811)<br /><br />
<p><br />
<p> <br />
<p>See SYSU-China 2011 iGEM Team Parts<br />
<p>&nbsp; </p><br />
<p><a href="http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2011&amp;group=SYSU-China">http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2011&amp;group=SYSU-China</a></p><br />
<br />
</div> <br />
</div><br />
</div><br />
<!---------------------------------------------!--><br />
<div class="page_content_RsideBar"><br />
<div class="page_content_videoBar"><br />
<br/><br />
<h1>Please watch our animination</h1><br />
<iframe width="307" height="225" src="http://www.youtube.com/embed/KymdcolAhcE?rel=0" frameborder="0" allowfullscreen></iframe><br />
<br />
<br/><br />
<h4>to see the full version of this animation,<a href="http://biogeek.kilu.de/animation.avi<br />
">click here to download</a> </h4><br />
<br/><br />
</div><br />
<div class="page_content_relatedBar_project_dp"><br />
<h2>Pages we think helpful:</h2><br />
<h1><a href="https://2011.igem.org/Team:SYSU-China/page_project_modules_construction">see how we construct our modules</a></h1><br />
<h1><a href="https://2011.igem.org/Team:SYSU-China/page_project_modules_verification">see how we verify each module</a></h1><br />
<h1><a href="https://2011.igem.org/Team:SYSU-China/main_page_story">or see our whole idea in Story page</a></h1><br />
</div><br />
</div> <br />
<!---------------------------------------------!--><br />
<div class="main_page_clearer_light"></div><br />
<!---------------------------------------------!--> <br />
<br />
</div><!--here ends the page_content_WholeBox!--><br />
<div class="main_page_clearer_light"></div><br />
</div><!--here ends the mainpage_project_WholeContainer!--><br />
<br />
<!--here begins the contact_bottombar!--><br />
<div class="contact_bottombar"><br />
<p>From the 2011 iGEM team SYSU-China (2011)</p><br />
<p>Sun Yat-Sen University, Guangzhou, China</p><br />
<p>Address: 135# Xingang Rd.(W.), Haizhu Guangzhou, P.R.China</p><br />
<p><a href="http://eng.sysu.edu.cn/">visit the Sun Yat-sen university website</a></p><br />
<p>Thanks Apycom jQuery Menus and <a href="http://apycom.com/">visit their website</a><br />
</div><br />
<!--here ends the contact_bottombar!--><br />
<br />
</div><!--here ends the backgroud_shadow_bar!--><br />
</body><br />
</html></div>Cambihttp://2011.igem.org/Team:SYSU-China/page_project_notesTeam:SYSU-China/page project notes2011-10-06T03:52:01Z<p>Cambi: </p>
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<!--------here begins the manu bar section--!--><br />
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<li><a href="index.thml"><span></span></a></li><br />
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<li><a href="https://2011.igem.org/Team:SYSU-China/main_page_story"><span>STORY</span></a></li> <br />
<li><a href="https://2011.igem.org/Team:SYSU-China/main_page_project" class="parent"><span>PROJECT</span></a><br />
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<li><a href="https://2011.igem.org/Team:SYSU-China/page_project_modules_construction"><span>Modules Construction</span></a><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/page_project_modules_verification"><span>Modules Verification</span></a></li><br />
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</li><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/main_page_human_practice" class="parent"><span>HUMAN PRACTICE</span></a><br />
<div><br />
<ul><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/page_human_practice_app"><span>App</span></a><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/page_human_practice_labcraft"><span>LabCraft Board Game</span></a></li><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/page_human_practice_workshop"><span>Workshop</span></a></li><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/page_human_practice_survey"><span>Survey</span></a></li><br />
</ul><br />
</div><br />
</li><br />
<br />
<li><a href="https://2011.igem.org/Team:SYSU-China/main_page_aboutus" class="parent"><span>ABOUT US</span></a><br />
<div><br />
<ul><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/page_aboutus_team_members"><span>Team Members</span></a><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/page_aboutus_special_thanks"><span>Spcial Thanks</span></a></li><br />
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<h2>Experiments Dairy</h2><br />
<br /><br /><br />
<li>July.8, 2011 Friday</li><br />
<li>Xu Yitian, Sun Weiwen, Zhao Zhilun: </li><br />
<p>Inoculate 10mL LB with the E. Coli BL21 plys strain.</P><br />
<br /><br />
<li>July.9, 2011 Saturday</li><br />
<li>Xu Yitian, Sun Weiwen, Zhao Zhilun: </li> <br />
<p>Preserve the E. Coli BL21 plys strain in 4℃ incubator. </P><br />
<br /><br />
<li>July.11, 2011 Monday</li><br />
<li>Xu Yitian, Sun Weiwen, Zhao Zhilun: </li> <br />
<p>1. Temperature gradient PCR of the two genes, trkD and cheZ, and two promoters, recA and recN promoters, according to their primers' Tm. </P> <br />
<br /><br />
<p>2. Result: The best PCR annealing temperature of trkD and cheZ are 60°and 45°respectively. The PCR of recA and recN promoters will be repeated tomorrow. </P> <br /><br />
<br />
<li>July.12, 2011 Tuesday</li><br />
<li>Xu Yitian, Sun Weiwen, Zhao Zhilun: </li> <br />
<p>1. PCR of trkD and cheZ to get large amount of these genes, but failed because of mistake of wrong PCR system. </P> <br /><br />
<p>2. PCR of recA and recN promoters, the best PCR temperature are both 60°. </P> <br /><br />
<p>3. Repeat the PCR of trkD and cheZ and extract pUC18 plasmid. Success!<br />
<li>July.13, 2011 Wednesday</li><br />
<li>Xu Yitian, Sun Weiwen, Zhao Zhilun: </li> <br />
<p>Cut the protective bases of the restriction sites of EcoRI and XbaI, and tried to link the promoters to the genes but failed. </P> <br /><br />
<li>July.16, 2011 Saturday</li><br />
<li>Xu Yitian, Sun Weiwen, Zhao Zhilun: </li> <br />
<p>Cut the plasmid with the enzyme of EcoRI, and link the promoters and genes and the plasmid together over night in 16°. </P> <br /><br />
<li>July.17, 2011 Sunday</li><br />
<li>Xu Yitian, Sun Weiwen, Zhao Zhilun: </li> <br />
<p>Eletrotransform the linked product to DH5a competence cells and revive with LB and culture them on the LB plate. However, there are no growing cells on the plate, which means our first try failed. </P> <br /><br />
<li>July.18, 2011 Monday</li><br />
<li>Xu Yitian, Sun Weiwen, Zhao Zhilun: </li> <br />
<p>PCR of the two promoters and the two genes, but during the gel extraction process, a small accident happened and the covers of the Ep tubes became powder, which blocked us from distinguish our four products. But we conducted a electrophoresis of our four products and we can differ our products. </P> <br /> <br />
<br />
<br />
<li>July.19, 2011 Tuesday</li><br />
<li>Xu Yitian, Sun Weiwen, Zhao Zhilun: </li> <br />
<p>Cut the protective bases of the restriction sites of EcoRI and XbaI, and cut the plasmid with the enzyme of EcoRI, and link the promoters and genes and the plasmid together over night in 16°. </P> <br /><br />
<li>July.20, 2011 Wednesday</li><br />
<li>Xu Yitian, Sun Weiwen, Zhao Zhilun: </li> <br />
<p>Eletrotransform the linked product to DH5a competence cells and revive with LB and culture them on the LB plate. However, there are no growing cells on the plate, which means our first try failed. </P> <br /><br />
<li>July.21, 2011 Thursday</li><br />
<li>Xu Yitian, Sun Weiwen, Zhao Zhilun: </li> <br />
<p>1. Use the PCR products two days ago and cut the restriction sites of them and linearize the plasmid, and link the promoters, the genes and the plasmid in 16° overnight. </P> <br /><br />
<p>2. Re-PCR recN since the first design of it has some mistake. </P> <br /><br />
<li>July.28, 2011 Thursday</li><br />
<li>Xu Yitian, Sun Weiwen, Zhao Zhilun: </li> <br />
<p>Successfully link recA promoter to the gene of cheZ (there are colonies on the plate and positive results of Conlony PCR). Then we sent the strain to Invitrogen to check its sequence if there is any base mutation. It turned out that the promoter and the gene are normal. Till now, we get our first useful plasmid: recA-cheZ-pUC18. </P> <br /><br />
<li>July.29, 2011 Friday</li><br />
<li>Xu Yitian, Sun Weiwen, Zhao Zhilun: </li> <br />
<p>We tried to construct the plasmid with GFP. We design the restriction sites of the GFP to be PstI and HindIII, and then we purified the GFP and linked it to the linearized pUC18. </P> <br /><br />
<br />
<li>July.30, 2011 Saturday</li><br />
<li>Xu Yitian, Sun Weiwen, Zhao Zhilun: </li> <br />
<p>1. Eletrotransform the linking product to DH5a competence cells, and grow them on plate. </P> <br /> <br />
<p>2. We continued to link recN to trkD. We PCR the two parts and cut them with EcoRI and XbaI, and cut the pUC18 plasmid with EcoRI. And links them at 16°overnight. </P> <br /><br />
<li>July.31, 2011 Sunday</li><br />
<li>Xu Yitian, Sun Weiwen, Zhao Zhilun: </li> <br />
<p>1. There is no colony on the plate. </P> <br /><br />
<p>2. Eletrotransform the linking product of recN and trkD to DH5a competence cells, and grow them on plate. </P> <br /> <br />
<li>Aug.1, 2011 Monday</li><br />
<li>Xu Yitian, Sun Weiwen, Zhao Zhilun: </li><br />
Colony PCR of the colonies on the plate of recN-trkD, however, we use the wrong primers and get no result. We corrected the mistake and get 4 positive results. We inoculate 2 conlonies into 5mL LB. </P> <br /><br />
<li>Aug.2, 2011 Tuesday</li><br />
<li>Xu Yitian, Sun Weiwen, Zhao Zhilun: </li><br />
<p>We sent the two strains to check sequence. </P> <br /><br />
<li>Aug.7, 2011 Sunday</li><br />
<li>Xu Yitian, Sun Weiwen, Zhao Zhilun: </li><br />
<p>The recN and trkD did not link into the plasmid by checking the sequencing result. </P> <br /><br />
<li>Aug.8, 2011 Monday</li><br />
<li>Xu Yitian, Sun Weiwen, Zhao Zhilun: </li><br />
<p>1. We ran a temperature gradient PCR of the new cheZ and trkD, both of which are without terminator because we want to link the two genes before the GFP and exam the gene expression by observe the fluorescence of GFP. However, the PCR of trkD(no terminator) failed while the cheZ(no termination ) succeed. </P> <br /> <br />
<p>2. Cut the plasmid of pUC18 with HindIII and PstI, then exam the product with the GFP gene. Then link the gene of GFP with the plasmid of pUC18. </P> <br /><br />
<br />
<br />
<li>Aug.9, 2011 Tuesday</li><br />
<li>Xu Yitian, Sun Weiwen, Zhao Zhilun: </li><br />
<p>Eletrotransform the linking product into DH5a cells and culture them on an Amp plate. Patch again after 12h culture. </P> <br /> <br />
<li>Aug.10, 2011 Wednesday</li><br />
<li>Xu Yitian, Sun Weiwen, Zhao Zhilun: </li><br />
<p>1. Colony PCR of the colonies on the plate of GFP-pUC18 and get 3 positive results. Inoculate 3 colonies into 5mL LB and place them in the shaker of 220rpm 37°overnight. </P> <br /> <br />
<p>2. PCR of CheZ and trkD, both of which are off terminators, and the two promoters. Then use enzymes to cut the protective sites of the genes and the restriction sites of the plasmid of GFP-pUC18. Then link recA, recN, cheZ, trkD respectively with the plasmid. </P> <br /> <br />
<br />
<li>Wang Zilong, Wang Li, Zheng Yi: </li><br />
<p>1. Double digest pUC18 and EGFP with HindIII and PstI, then ligate pUC18 and EGFP with T4 ligase in 16℃ over night. (1 is 1Kb marker, 2 is pUC18 and 3 is EGFP). </P> <br /><br />
<br />
<p>2. Gradient PCR to search the proper condition of cloning trkD and cheZ (for western blot). (Left side is cheZ, right side is trkD). </P> <br /><br />
<br />
<br />
<br />
<li>Aug.11, 2011 Thursday</li><br />
<li>Xu Yitian, Sun Weiwen, Zhao Zhilun: </li><br />
<p>1. Send the 3 GFP-pUC18 strains to Invitrogen to check their sequence. And we extract plasmid from the leaved inocula. </P> <br /><br />
<p>2. Eletrotransform the linking product into DH5a cells and culture them on Amp plates. Patch again after 12h culture. </P> <br /><br />
<br />
<li>Wang Zilong, Wang Li, Zheng Yi: </li><br />
<p>1. Transformation of pUC18-EGFP to DH5α. </P> <br /><br />
<p>2. Use KOD plus polymerase to amplifying trkD and cheZ (for western blot). </P> <br /><br />
<br />
<p>3. Double digest trkD and cheZ (for western blot) with EcoRI and HindIII, then ligate them to pET-28a and pET-32a which were digested with same enzymes. However, with few units of plasmids, this ligation had to be done in next day. </P> <br /><br />
<br />
<li>Aug. 12, 2011 Friday</li><br />
<li>Wang Zilong, Wang Li, Zheng Yi: </li><br />
<p>1. Re-digest pET-28a and pET-32a with EcoRI and HindIII, then ligate to trkD and cheZ (for western blot) with T4 ligase in 16℃ over night. (1 is cheZ, 2 is trkD, 3 is 1Kb marker, 4 is pET28a, 5 is pET-32a). </P> <br /><br />
<br />
<p>2. PCR trkD (for recombinant PCR), but failed. </P> <br /><br />
<p>3. Clony PCR pUC18-EGFP with Go Taq® to test the result of transformation. </P> <br /><br />
<br />
<br />
<li>Aug.14, 2011 Sunday</li><br />
<li>Xu Yitian, Sun Weiwen, Zhao Zhilun: </li><br />
<p>Patch the colonies on the plates and run a colony PCR and got several positive result .Inoculate the positive strain with 5mL LB respectively. </P> <br /><br />
<li>Aug.15, 2011 Monday</li><br />
<li>Xu Yitian, Sun Weiwen, Zhao Zhilun: </li><br />
<p>Send our samples to check their sequence. Then extract plasmids from those different strains. </P> <br /><br />
<li>Aug.16, 2011 Tuesday</li><br />
<li>Xu Yitian, Sun Weiwen, Zhao Zhilun: </li><br />
<p>Cut the different plasmids and exam whether these plasmids have genes in them. The cheZ-GFP-pUC18 and trkD-GFP-pUC18 do have the two genes on the plasmid, while the two promoters failed. </P> <br /> <br />
<br />
<li>Wang Zilong, Wang Li, Zheng Yi: </li><br />
<p>Try to use recombinant PCR to clone recN-trkD in one-step program and two-step program with Go Taq®, which helps to find the proper condition of PCR. </P> <br /><br />
<br />
<li>Aug. 17, 2011 Wednesday</li><br />
<li>Wang Zilong, Wang Li, Zheng Yi: </li><br />
<p>1. Digest plasimds cheZ-pET-32a and trkd-pET-32a with EcoRI and double digest with EcoRI and HindIII to test whether their lengths are correct, and the result shows trkd-pET-32a is correctly, but cheZ-pET-32a is not. </P> <br /><br />
<br />
<p>2. Try to use recombinant PCR to clone recN-trkD in two-step program with Go Taq®, which helps to find the proper condition of PCR. </P> <br /><br />
<br />
<li>Aug. 19, 2011 Friday</li><br />
<li>Wang Zilong, Wang Li, Zheng Yi: </li><br />
<p>Induce cheZ-pET-32a and trkd-pET-32a with 0.1, 0.5, 1.0 mM IPTG in OD 0.600. </P> <br /><br />
<li>Aug. 20, 2011 Saturday</li><br />
<li>Wang Zilong, Wang Li, Zheng Yi: </li><br />
<p>In SDS-PAGE, use proteins in liquid and lysate expressed by cheZ-pET-32a and trkd-pET-32a to do electrophoresis. And the gel with proteins in liquid does western blot, while the other gel is dyed with G250. (1~4 are trkd-pET-32a induced by 0, 0.1, 0.5, 1.0 mM IPTG, 5 is marker, 6~9 are cheZ-pET-32a induced by 0, 0.1, 0.5, 1.0 mM IPTG). </P> <br /><br />
<br />
<li>Aug.21, 2011 Sunday</li><br />
<li>Xu Yitian, Sun Weiwen, Zhao Zhilun: </li><br />
<p>We inoculated the cheZ-GFP-pUC18 and trkD-GFP-pUC18 with 5mL LB. We use IPTG to trigger the lac promoter before both the cheZ-GFP-pUC18 and trkD-GFP-pUC18, then use fluorescence microscope to exam whether the two genes have successfully expressed. </P> <br /> <br />
<li>Aug.22, 2011 Monday</li><br />
<li>Xu Yitian, Sun Weiwen, Zhao Zhilun: </li><br />
<p>We intermediatecultured 1ml strains with 100mL LB. After two hours shaking and measured the OD value of the two strains of 0.45, we separated the 100ml inocula into 4 conical flasks and induced with 3 different concentrations of IPTG(one leaved is control). After 4 hours induction, we used fluorescence microscope to exam the inocula and find out that the control group, which should not be of a dark view, have fluorescence too. That means the lac promoter can trigger the expression of its downstream genes without induction. Then we want to transfer our genes onto another plasmid, pET28a, whose promoter lied between the restriction sites of BamHI and BglII. The two enzymes are isocaudamers and the gap cut by them can be linked without inserting any sequence. </P> <br /> <br />
<br />
<li>Wang Zilong, Wang Li, Zheng Yi: </li><br />
<p>Clone recN-trkD recombination in recombinant PCR program with PrimeStar®. After double digest the gene with EcoRI and PstI, ligate it with plasmid pUC18-EGFP digested with same enzymes. (1 is 1Kb marker, 2 is recN-trkD, 3 is pUC18-EGFP). </P> <br /><br />
<br />
<li>Aug.23, 2011 Tuesday</li><br />
<li>Xu Yitian, Sun Weiwen, Zhao Zhilun: </li><br />
<p>1. Cut the plasmid of GFP-pUC18 and the protective sites of recN and recA with the enzyme of XbaI and EcoRI, but the plasmid is so little amount that it cannot be seen on the gel. Inoculate the strain with the plasmid of GFP-pUC18. </P> <br /><br />
<p>2. Use BamHI and BglII to cut the plasmid of pET28a, and use T4 ligation enzyme to link the gap cut by the two enzymes of the plasmid. </P> <br /><br />
<li>Aug.24, 2011 Wednesday</li><br />
<li>Xu Yitian, Sun Weiwen, Zhao Zhilun: </li><br />
<p>1. Eletrotransform the linking product into DH5a cells and culture them on Amp plates. Patch again after 12h culture. Wait 12h but no colony found. </P> <br /><br />
<p>2. Extract plasmid from the inocula. recut the plasmid and get a clear line. Then try to link recA and recN onto the plasmid. 16°overnight. </P> <br /><br />
<li>Aug.25, 2011 Thursday</li><br />
<li>Xu Yitian, Sun Weiwen, Zhao Zhilun: </li><br />
<p>1. Repeat the experiment of pET28a promoter cutting. </P> <br /><br />
<p>2. Eletrotransform the linking product into DH5a cells and culture them on Amp plates. Patch again after 12h culture. </P> <br /> <br />
<li>Aug.26, 2011 Friday</li><br />
<li>Xu Yitian: </li><br />
<p>NO COLONY FOUND!!!!! </P> <br /><br />
<li>Sept.1, 2011 Thursday</li><br />
<li>Wang Li: </li><br />
<p>Western Blot</P> <br /><br />
<br />
<li>Sept.6, 2011 Tuesday</li><br />
<li>Sun Weiwen: </li><br />
<p>1. Transfer the pET32 plasmid into E.coli BL21. </P> <br /><br />
<p>2. Culture the pET32a-BL21 E.coli and the trkD-pET32a-BL21 E.coli with 5ml LB medium. </P> <br /><br />
<p>3. Put 3ml culture of the pET32a-BL21 E.coli and the trkD-pET32a-BL21 E.coli into 100ml CsCl-LB solution (CsCl content: 0.4g/100ml LB) respectively. Two hours later, their respective OD values are 0.35 and 0.53. Then induce them with 500μl IPTG(concentration: 0.1mol/L). Four and a half hours later, their respective OD values are 1.03 and 1.14. Then keep them still in the 37℃ incubator for 4 hours. Centrifugate the culture to collect the E.coli, then wash them with PBS for two times. In the end, suspend the E.coli with 20ml ddH2O respectively. Then send the two samples of the pET32a-BL21 E.coli and the trkD-pET32a-BL21 E.coli to China National Analytical Center, Guangzhou for the analysis of Cs element. </P> <br /><br />
<br />
<li>Wang Li: </li><br />
<p>Western Blot</P> <br /><br />
<br />
<li>Sept. 7, 2011 Wednesday</li><br />
<li>Wang Zilong, Zheng Yi: </li><br />
<p>1. Transform pSB1C3, pSB1A3, pSB1T3 and BBa_J04450 to DH5α. </P> <br /><br />
<p>2. Transform cheZ-pET-28a to strains whose cheZ gene is knocked out, and smear them on the medium with IPTG line to test chemotaxis. </P> <br /><br />
<li>Zhao Zhilun: </li><br />
<p>1. Double digest gfp-pUC18 and recA-cheZ(without terminator) with EcoRI and PstI in the 30μl system. Ligate gfp-pUC18 with recA-cheZ(without terminator) overnight. Electrotransform the ligation product into E.coli DH5α. Smear them on the solid medium containing Ampicillin. </P> <br /> <br />
<p>2. Double digest pET28a with BamHI and BglII, and then ligate itself with T4 ligase overnight.<br />
<p>3. Use colony PCR to detect whether recA-gfp-pUC18-infusion is successfully constructed. The result indicates that it fails. </P> <br /><br />
<p>4. Sequence the only grown E.coli in yesterday which contain recA-gfp-pUC18-infusion. </P> <br /> <br />
<li>Sept. 8, 2011 Thursday</li><br />
<li>Wang Zilong, Zheng Yi: </li><br />
<p>Do PCR on recN and trkD (for biobrick assembling) with KOD plus® polymerase, but failed. </P> <br /><br />
<br />
<li>Zhao Zhilun: </li><br />
<p>1. Electrotransform the self-ligation product of pET28a into DH5α, and smear them on the solid medium. </P> <br /><br />
<p>2. Result of colony PCR shows that I fail to construct recA-GFP-pUC18 in-fusion. </P> <br /><br />
<li>Sept. 9, 2011 Friday</li><br />
<li>Wang Zilong, Zheng Yi: </li><br />
<p>1. Do gradient PCR to clone recN and trkD (for biobrick assembling) with Go Taq®, but failed again. </P> <br /><br />
<p>2. Try to use cheZ-pET-28a to test chemotaxis again. </P> <br /><br />
<br />
<li>Wang Li: </li><br />
<p>Western Blot</P> <br /><br />
<br />
<li>Sept.10, 2011 Saturday</li><br />
<li>Sun Weiwen: </li><br />
<p>1. Culture the E.coli with cheZ-gfp-pUC18 and trkD-gfp-pUC18 with 5ml LB medium overnight. </P> <br /><br />
<p>2. Expand the culture with 100ml LB medium containing ampicillin. Two hours later, both of their OD values are 0.54. Then divide the 100ml culture into two bottles of 50ml, and induce one of the two with IPTG, keeping the other as the control. Four hours later, observe the expression of EGFP with confocal laser scanning microscopy. The result is that the experiment group is brighter than the control group. </P> <br /><br />
<br />
<li>Wang Zilong, Zheng Yi: </li><br />
<p>1. Use Go Taq® to PCR recN again, but failed again. </P> <br /><br />
<p>2. Collect plasmid pSB1C3 to test the length of it. </P> <br /><br />
<li>Sept. 11, 2011 Sunday</li><br />
<li>Wang Zilong, Zheng Yi: </li><br />
<p>1. Digest plasmid pSB1C3 with EcoRI, SpeI, XbaI and PstI, respectively, to test the plasmid. (1 and 6 is 1Kb marker, 2~5 are pSB1C3 digested by EcoRI, SpeI, XbaI and PstI, respectively). </P> <br /><br />
<br />
<p>2. Use new template bacterial to PCR recN and trkD (for biobrick assembling) with Go Taq®, and succeed in time; then amplifying cloning the genes. </P> <br /><br />
<br />
<br />
<li>Sept.12 Monday</li><br />
<li>Wang Li: </li><br />
<p>Western Blot</P> <br /><br />
<br />
<li>Sept.13, 2011 Tuesday</li><br />
<li>Sun Weiwen: </li><br />
<p>1. Continue Zhao's experiment to pick the single colony of infusion recA-gfp-pUC18-DH5α, pET28a-T7 promoter knock-out-DH5α, and recA-cheZ(no ter)-gfp-pU18-DH5α. When the colony grows up, conduct the colony PCR of infusion recA-gfp-pUC18-DH5α and recA-cheZ(no ter)-gfp-pU18-DH5α, but there are no expected results. Extract the plasmids of pET28a-T7 promoter knock-out-DH5α, and then digest them with SmaI and XbaI, the system is as following:<br />
ddH2O: 15μl<br />
Buffer 4: 3μl<br />
Plasmid: 10μl<br />
SmaI: 1μl<br />
XbaI: 1μl<br />
In the end, there is also no expected result. </P> <br /><br />
<br />
<li>Wang Zilong, Zheng Yi: </li><br />
<p>Double digest recN, trkD (for biobrick assembling) and plasmid pSB1C3 with XbaI and SpeI, and then ligate the genes to the plasmid. (1 is 1Kb marker, 2 is recN, 3 is trkD, 4 is pSB1C3). </P> <br /><br />
<br />
<li>Sept. 15, 2011 Thursday</li><br />
<li>Wang Zilong, Zheng Yi: </li><br />
<p>Double digest recA, recN and plasmid pUC18-EGFP with XbaI and EcoRI-HF, and then ligate the genes to the plasmid. </P> <br /><br />
<br />
<li>Sept.16, 2011 Friday</li><br />
<li>Sun Weiwen: </li><br />
<p>1. Receive the Cs analysis report of last experiment, and the results are as following:<br />
Control: 23.9mg/Kg (sample weight: about 20g)<br />
Experiment: 32.8mg/Kg (sample weight: about 20g). </P> <br /><br />
<p>2. Repeat the experiments from 2011-9-4 to 2011-9-6, but the original Cs concentration is 0.05g CsCl/100ml LB. Induce the experiment group with IPTG at 18℃ overnight. Then keep them still in the 37℃ incubator for 6 hours. Collect the E.coli for analysis. </P> <br /><br />
<br />
<li>Wang Zilong, Zheng Yi: </li><br />
<p>Transform recA-pUC18-EGFP and recN-pUC18-EGFP to DH5α. </P> <br /><br />
<br />
<li>Sept.17, 2011 Saturday</li><br />
<li>Sun Weiwen: </li><br />
<p>1. Find out that nalidixic acid or mitomycin C have the same effect on Promoter recA or recN. </P> <br /><br />
<p>2. Plan to ligate recA with trkD if the ligation of recN and trkD cannot be accomplished. </P> <br /><br />
<p>3. T7 promoter of pET28a has been cut off. </P> <br /><br />
<br />
<li>Wang Zilong, Zheng Yi: </li><br />
<p>Do Clony PCR on recN-pSB1C3, trkD-pSB1C3, recA-pUC18-EGFP and recN-pUC18-EGFP to test the ligation. </P> <br /><br />
<br />
<br />
<li>Sept. 18, 2011 Sunday</li><br />
<li>Wang Zilong, Zheng Yi: </li><br />
<p>1. Transform cheZ-pET-28a to strain whose cheZ gene is knocked out. </P> <br /><br />
<p>2. Collect plasmid recN-pSB1C3, trkD-pSB1C3, recA-pUC18-EGFP and recN-pUC18-EGFP and double digest the first one with EcoRI and SpeI, second one with EcoRI and XbaI, third one and forth one with EcoRI and HindIII, which shows the false-positive result of recN-pSB1C3, trkD-pSB1C3. </P> <br /><br />
<br />
<br />
<li>Wang Li: </li><br />
<p>Western Blot</P> <br /><br />
<br />
<li>Sept. 19, 2011 Monday</li><br />
<li>Wang Zilong, Zheng Yi: </li><br />
<p>Re-ligate recN and trkD to pSB1C3 and then transform them into DH5α. After culture over night, do clony PCR to test whether the ligation is successful, and both are successful. (1 is 1Kb marker, 2 is recN, 3 is trkD, 4 is pSB1C3). </P> <br /><br />
<br />
<br />
<li>Sept.21, 2011 Wednesday</li><br />
<li>Sun Weiwen: </li><br />
<p>1. Receive the Cs analysis report of last experiment, and the results are as following:<br />
Control: 5.58mg/Kg (sample weight: 23.22g).<br />
Experiment: 5.38mg/Kg (sample weight: 21.17g). </P> <br /><br />
<p>2. Repeat the experiments from 2011-9-4 to 2011-9-6, but the original Cs concentration is 0.6g CsCl/100ml LB. Induce the experiment group with IPTG at 25℃ overnight. Then keep them still in the 37℃ incubator for 6 hours. Collect the E.coli for analysis. </P> <br /><br />
<p>3. Receive the Cs analysis report of last experiment, and the results are as following:<br />
Control: 112mg/Kg (sample weight: 19.20g)<br />
Experiment: 77.9mg/Kg (sample weight: 20.94g). </P> <br /><br />
<li>Sept. 25, 2011 Sunday</li><br />
<li>Wang Zilong, Zheng Yi: </li><br />
<p>Use Go Taq® to PCR trkD (for ligation with recN-pUC18-EGFP). </P> <br /><br />
<br />
<br />
<li>Wang Li: </li><br />
<p>Western Blot Succeed</P> <br /><br />
<br />
<li>Sept. 26, 2011 Monday</li><br />
<li>Wang Zilong, Zheng Yi: </li><br />
<p>1. Amplifying cloning of trkD (for ligation with recN-pUC18-EGFP) with PrimeStar®. </P> <br /><br />
<br />
<p>2. Double digest pSB1C3 with EcoRI and PstI. </P> <br /><br />
<p>3. Gradient PCR genes (recA1, recN2, cheZ3, cheZ'4, trkD5, trkD'6, EGFP7) for biobrick assembling with Go Taq® to search proper condition of PCR. </P> <br /><br />
<br />
<p>4. Double digest trkD (for ligation with recN-pUC18-EGFP) with XbaI and HindIII. </P> <br /><br />
<br />
<li>Sept. 27, 2011 Tuesday</li><br />
<li>Wang Zilong, Zheng Yi: </li><br />
<p>1. Amplifying cloning of genes for biobrick assembling with PrimeStar®. </P> <br /><br />
<br />
<br />
<p>2. Double digest genes for biobrick assembling with EcoRI and PstI, and then ligate them with pSB1C3 digested by same restrictive enzymes. (1~7 are pSB1C3, recA, recN, cheZ, cheZ', trkD, EGFP). </P> <br /><br />
<br />
<br />
<br />
<li>Wang Li: </li><br />
<p>Repeat Western Blot</P> <br /><br />
<br />
<li>Sept. 28, 2011 Wednesday</li><br />
<li>Wang Zilong, Zheng Yi: </li><br />
<p>Transform products of ligation into DH5α, and the results shows the failure of the ligation. And then do this ligation again with new enzymes. </P> <br /><br />
<br />
<li>Sept.30, 2011 Friday</li><br />
<li>Sun Weiwen: </li><br />
<p>1. Zhao has constructed the plasmid recA-gfp-pET28a(T7 knock-out) and recA-gfp-pET28a(T7 knock-out), So I cut of the gfp gene from the two kinds of plasmid. </P> <br /><br />
<p>2. Try to ligate trkD-gfp or only trkD into the two kinds of plasmid. Although some E.coli could grow on the culture containing antibiotic, the results of colony PCR are not expected. In the end, all tries to ligate trkD-gfp or only trkD into the plasmids recA-gfp-pET28a (T7 knock-out) and recA-gfp-pET28a (T7 knock-out) have failed. </P> <br /><br />
<br />
<li>Oct. 1, 2011 Saturday</li><br />
<li>Wang Zilong, Zheng Yi: </li><br />
<p>Re-clone all the genes again and digest them again, then ligate them with plasmid again to test whether the ligation is successful. Do transformation. (1~8 are recA, recN, EGFP, pSB1C3, cheZ, cheZ', trkD, trkD', respecitvely). </P> <br /><br />
<br />
<li>Xu Yitian, Zhao Zhilun: </li><br />
<p>Inoculate two strains: recA-GFP-pET28a(T7 knock-out)-BL21 and recN-GFP-pET28a(T7 knock-out)-BL21. </P> <br /><br />
<li>Oct. 2, 2011 Sunday</li><br />
<li>Wang Zilong, Zheng Yi: </li><br />
<p>Double digest genes for biobrick assembling with XbaI and SpeI, and then ligate them with pSB1C3 digested by same restrictive enzymes. Do transformation. (1~8 are EGFP, trkD, pSB1C3, recA, recN, cheZ, trkD', cheZ', respectively). </P> <br /><br />
<br />
<br />
<li>Wang Li: </li><br />
<p>Exploring E.coli movement induced by NAL or UV. </P> <br /><br />
<br />
<li>Xu Yitian, Zhao Zhilun: </li><br />
<p>1. Mix 1.4 ml each inocula with 70ml LB, then place them into the 37°220rpm shaker. Separate every 10 ml inocula into an aseptic conical flask, and induce with a specific concentration of NAL. There are 6 experiment groups and one control group. Place all the conical flasks into shaker at 37°220rpm for 4h. Then add 200ul to every hole of a microplate and exam the fluorescence intensity by the Microplate Reader. </P> <br /><br />
<p>2. Inoculate the same stains as last night. </P> <br /><br />
<br />
<li>Oct.3, 2011 Monday</li><br />
<li>Sun Weiwen: </li><br />
<p>1. Digest recA-gfp-pET28a (T7 knock-out) and gene trkD with XbaI and HindIII respectively, and then to ligate them together. </P> <br /> <br />
<p>2. Electrotransform the ligation product into E.coli DH5α, and smear them on the solid medium containing Kanamycin. After the colony grows up, pick up the single colony to smear on another medium. After 6 hours' culture, the colony grows up, and then conduct the colony PCR to copy the gene trkD. The result indicates that I have successfully construct the recA-trkD-pET28a (T7 knock-out) plasmid. </P> <br /> <br />
<br />
<br />
<li>Xu Yitian: </li><br />
<p>1. Mix 3.4 ml each inocula with 170ml LB, then place them into the 37°220rpm shaker. Separate every 10 ml inocula into an aseptic conical flask, and induce with a specific concentration of NAL. There are 6 experiment groups and one control group. Place all the conical flasks into shaker at 37°220rpm for 4h. Then regulate OD value of the inocula in every conical flask to 2.023 to 2.033. The remaining 100ml incula was separated to 20 centrifuge tubes. 5ml inocula in every tube. Place all the tubes under the UV light. Take out one tube every 10min and mark the time on the tube. Then add 200ul to every hole of a microplate and exam the fluorescence intensity by the Microplate Reader. </P> <br /><br />
<p>2. Inoculate three stains: the same stains as last night and the strain of BL21. </P> <br /><br />
<li>Oct.4, 2011 Tuesday</li><br />
<p>Mix 1.4 ml each inocula with 70ml LB, then place them into the 37°220rpm shaker. Separate every 10 ml inocula into an aseptic conical flask, and induce with a specific <br />
<h2>Our Protocols</h2><br />
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<p>Please click <a href="https://static.igem.org/mediawiki/igem.org/5/5f/Protocols_SYSU.pdf">HERE</a> to see or download our protocols in PDF format</p><br />
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<h1><a href="https://static.igem.org/mediawiki/igem.org/5/5f/Protocols_SYSU.pdf">Download our protocols</a></h1><br />
<br />
<h1><a href="https://2011.igem.org/Team:SYSU-China/page_project_modules_construction">see how we construct our modules</a></h1><br />
<h1><a href="https://2011.igem.org/Team:SYSU-China/page_project_modules_verification">see how we verify each module</a></h1><br />
<h1><a href="https://2011.igem.org/Team:SYSU-China/main_page_story">or see our whole idea in Story page</a></h1><br />
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<p>From the 2011 iGEM team SYSU-China (2011)</p><br />
<p>Sun Yat-Sen University, Guangzhou, China</p><br />
<p>Address: 135# Xingang Rd.(W.), Haizhu Guangzhou, P.R.China</p><br />
<p><a href="http://eng.sysu.edu.cn/">visit the Sun Yat-sen university website</a></p><br />
<p>Thanks Apycom jQuery Menus and <a href="http://apycom.com/">visit their website</a><br />
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</html></div>Cambihttp://2011.igem.org/Team:SYSU-China/page_project_functional_constructionTeam:SYSU-China/page project functional construction2011-10-06T03:51:20Z<p>Cambi: </p>
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<li><a href="https://2011.igem.org/Team:SYSU-China/page_project_modules_construction"><span>Modules Construction</span></a><br />
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<p>&nbsp;&nbsp;&nbsp;&nbsp;After the modules verification, we began further tests of our constructed bacteria to verify that the genes can function well.</p><br />
<p>&nbsp;&nbsp;&nbsp;&nbsp;First, we tested the function of our constructed gene cheZ. Because of limited conditions, we tried to replace ion radiation with common UV light, which has the same effect of DNA damage to activate the SOS signal pathway. When exposed to a certain intensity(which couldn't be measured for a lack of apposite apparatus) of UV light, the E.coli transformed with RecA-CheZ-pET28a was observed a faster movement towards it. </p><br />
<p>&nbsp;&nbsp;&nbsp;&nbsp;Then, we used TrkD-pET32a to test the function of trkD, which could be accomplished through detection of the accumulation of Cs+ in E.coli extract. The results indicate that within the inducement of 0.5mM IPTG at 37℃ for 4h, the experimental group has a significantly higher amount of Cs+ absorption than the control group. </p><br />
<p>&nbsp;&nbsp;&nbsp;&nbsp;In terms of the results above, we have proved that cheZ and trkD constructed in the plasmids functions. Taking these two evidence together, it's not hard to predict that the constructed bacteria obtain the ability to 'swim' towards ion radiation and to absorb Cs+. </p><br />
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<h4>to see the full version of this animation,<a href="http://biogeek.kilu.de/animation.avi<br />
">click here to download</a> </h4><br />
<br/><br />
<br />
</div><br />
<div class="page_content_relatedBar_project_fv"><br />
<h2>Pages we think helpful:</h2><br />
<h1><a href="https://2011.igem.org/Team:SYSU-China/page_project_modules_construction">see how we construct our modules</a></h1><br />
<h1><a href="https://2011.igem.org/Team:SYSU-China/page_project_modules_verification">see how we verify each module</a></h1><br />
<h1><a href="https://2011.igem.org/Team:SYSU-China/main_page_story">or see our whole idea in Story page</a></h1><br />
<p>&nbsp;</p><br />
</div><br />
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<br />
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<!--here begins the contact_bottombar!--><br />
<div class="contact_bottombar"><br />
<p>From the 2011 iGEM team SYSU-China (2011)</p><br />
<p>Sun Yat-Sen University, Guangzhou, China</p><br />
<p>Address: 135# Xingang Rd.(W.), Haizhu Guangzhou, P.R.China</p><br />
<p><a href="http://eng.sysu.edu.cn/">visit the Sun Yat-sen university website</a></p><br />
<p>Thanks Apycom jQuery Menus and <a href="http://apycom.com/">visit their website</a><br />
</div><br />
<!--here ends the contact_bottombar!--><br />
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</html></div>Cambihttp://2011.igem.org/Team:SYSU-China/page_project_modules_constructionTeam:SYSU-China/page project modules construction2011-10-06T03:47:25Z<p>Cambi: </p>
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<li><a href="https://2011.igem.org/Team:SYSU-China/page_project_modules_construction"><span>Modules Construction</span></a><br />
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<div id="page_content_baBar_details"><a href="#page_content_textBar_enterTEXT">See Details</a></div><br />
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<p>&nbsp;&nbsp;&nbsp;&nbsp;We have designed three categories of modules - Expressional Testing Modules, Functionalizing Testing Modules and Final Functional Modules - to test the expression and function of every element and to accomplish the final function. The followings are the details of these modules.</p><br />
<br /><br />
<h2>1. Expression Testing Modules</h2><br />
<br /><br />
<p>&nbsp;&nbsp;&nbsp;&nbsp;In order to test whether the elements we obtain from E.coli genome PCR can express normally, we have constructed the expressional testing modules through genetic engineering methods. But the existing promoter in the pET series plasmids will affect the expression test of promoter RecA and RecN, so we cut off the T7 promoter of the plasmid pET28a. By digesting promoter RecA and RecN with restriction endonuclease EcoRI and XbaI, digesting EGFP gene with PstI and HindIII, and then ligating them into plasmid pET28a, pET32a and pUC18 with T4 ligase, the following testing modules have been successfully constructed:</p><br />
<br /><br />
<li>a) RecA-gfp-pET28a(promoter knock-out) (figure.1)</li><br />
<li>b) RecN-gfp-pET28a(promoter knock-out) (figure.2)</li><br />
<li>c) CheZ(no terminator)-gfp-pUC18 (figure.3)</li><br />
<li>d) TrkD(no terminator)- gfp-pUC18 (figure.4)</li><br />
<li>e) CheZ-pET32a (figure.5)</li><br />
<li>f) TrkD-pET32a (figure.6)</li><br />
<br /><br />
<p>&nbsp;&nbsp;&nbsp;&nbsp;Through the appearance of the green fluorescence with the first four modules, we can know whether the elements RecA, RecN, CheZ and TrkD express normally as we expected. With the Western-blot tests using the modules CheZ-pET32a and TrkD-pET32a, we can directly know the expression state of CheZ and TrkD.</p><br />
<br /><br />
<h2>2. Functionalizing Testing Modules</h2><br />
<br /><br />
<p>&nbsp;&nbsp;&nbsp;&nbsp;For the purpose of realizing the function that CheZ and TrkD can start to express under the induction of nuclear radiation, we expected to ligate RecA with CheZ, and RecN with TrkD. But the ligation of RecN and TrkD was of great difficulty to be accomplished. This may be caused by the facts that RecN is too short and the overlapping zone of RecN and TrkD has far less than 15 base pairs, which is essential for recombinant PCR. However, TrkD expression under the promotion of RecA satisfies our requirements. As a result, we have constructed the following modules to conduct the functionalizing tests:</p><br />
<br /><br />
<li>a) RecA-CheZ-pET28a(promoter knock-out) (figure.7)</li><br />
<br />
<li>b) RecA-TrkD-pET28a(promoter knock-out) (figure.8)</li><br />
<li>c) RecA- CheZ(no terminator)-gfp-pET28a(promoter knock-out) (figure.9)</li><br />
<li>d) RecA- TrkD(no terminator)-gfp-pET28a(promoter knock-out) (figure.10)</li><br />
<br /><br />
<p>&nbsp;&nbsp;&nbsp;&nbsp;In addition to the modules constructed above, we also intend to construct the following module:</p><br />
<br /><br />
<li>e) RecN-TrkD(no terminator)-ag43- pET28a(promoter knock-out)</li><br />
<br /><br />
<p>&nbsp;&nbsp;&nbsp;&nbsp;With the first two modules, we could test the expression of CheZ and TrkD through the directed motion of E.coli and the Cs+ absorption under the induction of radiation. With the next two modules, the expression of CheZ and TrkD under the radiation induction can be reflected directly through the fluorescence of gfp. With the last module, we could test the expression state of ag43 through whether E.coli cells connect with each other.</p><br />
<br /><br />
<h2>3. Final Functional Modules</h2><br />
<br /><br />
<p>&nbsp;&nbsp;&nbsp;&nbsp;Based on the former construction of plasmids, we expect to integrate the module RecA-CheZ and RecA-TrkD-ag43 in the same E.coli to fulfill the final function that the E.coli could swimming to the source of nuclear leakage and absorb the radioactive Cs+ under the induction of radiation. At the same time, the ag43 in the downstream of TrkD starts to express. When the ag43 protein accumulates above the threshold, E.coli cells could cross link each other, and then be collected to clear the radioactive.<br />
</p> <br />
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<li> <a href="https://static.igem.org/mediawiki/2011/3/3b/SYSU_Figure.1_recA-GFP-pET28.png" title=""><img src="https://static.igem.org/mediawiki/igem.org/7/73/Figure.1_recA-GFP-pET28_S.png" width="150" height="97" /></a><br />
<br />
<h1>figure.1</h1><br />
<p>&nbsp;&nbsp;&nbsp;&nbsp;RecA-gfp-pET28a(promoter knock-out) <p></li><br />
<li> <a href="https://static.igem.org/mediawiki/2011/9/92/SYSU_Figure.2_recN-GFP-pET28.png" title=""><img src="https://static.igem.org/mediawiki/igem.org/f/f3/Figure.2_recN-GFP-pET28_S.png" width="150" height="97" /></a><br />
<h1>figure.2</h1><br />
<p>&nbsp;&nbsp;&nbsp;&nbsp;RecN-gfp-pET28a(promoter knock-out) </p><br />
</li><br />
<li> <a href="https://static.igem.org/mediawiki/2011/6/66/SYSU_Figure.3_lac-CheZ-GFP-pUC18.png" title=""><img src="https://static.igem.org/mediawiki/igem.org/5/50/Figure.3_lac-CheZ-GFP-pUC18_S.png" width="150" height="76" /></a><br />
<h1>figure.3</h1><br />
<p>&nbsp;&nbsp;&nbsp;&nbsp;CheZ(no terminator)-gfp-pUC18</p></li><br />
<li> <a href="https://static.igem.org/mediawiki/2011/c/ce/SYSU_Figure.4_lac-TrkD-GFP-pUC18.png" title=""><img src="https://static.igem.org/mediawiki/igem.org/9/94/Figure.4_lac-TrkD-GFP-pUC18_S.png" width="150" height="76"/></a><br />
<h1>figure.4</h1><br />
<p>&nbsp;&nbsp;&nbsp;&nbsp;TrkD(no terminator)- gfp-pUC18</p></li><br />
<li> <a href="https://static.igem.org/mediawiki/2011/8/8f/SYSU_Figure.5_T7-Chez-pET32a.png" title=""><img src="https://static.igem.org/mediawiki/igem.org/5/5e/Figure.5_T7-Chez-pET32a_S.png" width="150" height="97" /></a><br />
<h1>figure.5</h1><br />
<p>&nbsp;&nbsp;&nbsp;&nbsp;CheZ-pET32a</p></li><br />
<li> <a href="https://static.igem.org/mediawiki/2011/0/0d/SYSU_Figure.6_T7-TrkD-pET32a.png" title=""><img src="https://static.igem.org/mediawiki/igem.org/f/fe/Figure.6_T7-TrkD-pET32a_S.png" width="150" height="97" /></a><br />
<h1>figure.6</h1><br />
<p>&nbsp;&nbsp;&nbsp;&nbsp;TrkD-pET32a</p></li><br />
<li> <a href="https://static.igem.org/mediawiki/2011/e/e5/SYSU_Figure.7_recA-CheZ.png" title=""><img src="https://static.igem.org/mediawiki/igem.org/5/5f/Figure.7_recA-CheZ_S.png" width="150" height="97"/></a><br />
<h1>figure.7</h1><br />
<p>&nbsp;&nbsp;&nbsp;&nbsp;RecA-CheZ-pET28a(promoter knock-out)</p></li><br />
<li> <a href="https://static.igem.org/mediawiki/2011/1/13/SYSU_Figure.8_recA-TrkD.png" title=""><img src="https://static.igem.org/mediawiki/igem.org/9/99/Figure.8_recA-TrkD.png" width="150" height="97" /></a><br />
<h1>figure.8</h1><br />
<p>&nbsp;&nbsp;&nbsp;&nbsp;RecA-TrkD-pET28a(promoter knock-out)</p></li><br />
<li> <a href="https://static.igem.org/mediawiki/2011/9/90/SYSU_Figure.9_recA-CheZ-GFP-pET28.png" title=""><img src="https://static.igem.org/mediawiki/igem.org/b/b8/Figure.9_recA-CheZ-GFP-pET28_S.png" width="150" height="76" /></a><br />
<h1>figure.9</h1><br />
<p>&nbsp;&nbsp;&nbsp;&nbsp;RecA- CheZ(no terminator)-gfp-pET28a(promoter knock-out)</p></li><br />
<li> <a href="https://static.igem.org/mediawiki/2011/9/90/SYSU_Figure.10_recA-trkd-GFP-pET28.png" title=""><img src="https://static.igem.org/mediawiki/igem.org/e/e0/Figure.10_recA-trkd-GFP-pET28.png" width="150" height="76" /></a><br />
<h1>figure.10</h1><br />
<p>&nbsp;&nbsp;&nbsp;&nbsp;RecA- TrkD(no terminator)-gfp-pET28a(promoter knock-out)</p></li><br />
<br />
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<iframe width="307" height="225" src="http://www.youtube.com/embed/KymdcolAhcE?rel=0" frameborder="0" allowfullscreen></iframe><br />
<br />
<br/><br />
<h4>to see the full version of this animation,<a href="http://biogeek.kilu.de/animation.avi<br />
">click here to download</a> </h4><br />
<br/><br />
</div><br />
<div class="page_content_relatedBar_project_mv"><br />
<h2>Pages we think helpful:</h2><br />
<h1><a href="https://2011.igem.org/Team:SYSU-China/page_project_modules_verification">see how we verify each module</a></h1><br />
<h1><a href="https://2011.igem.org/Team:SYSU-China/main_page_story">or see our whole idea in Story page</a></h1><br />
<p>&nbsp;</p><br />
</div><br />
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<p>From the 2011 iGEM team SYSU-China (2011)</p><br />
<p>Sun Yat-Sen University, Guangzhou, China</p><br />
<p>Address: 135# Xingang Rd.(W.), Haizhu Guangzhou, P.R.China</p><br />
<p><a href="http://eng.sysu.edu.cn/">visit the Sun Yat-sen university website</a></p><br />
<p>Thanks Apycom jQuery Menus and <a href="http://apycom.com/">visit their website</a><br />
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</html></div>Cambihttp://2011.igem.org/Team:SYSU-China/main_page_storyTeam:SYSU-China/main page story2011-10-06T03:46:37Z<p>Cambi: </p>
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<h6>Story</h6><br />
<p>&nbsp;</p><br />
<h6>1. Background</h6><br />
<p>&nbsp;&nbsp;&nbsp;&nbsp;On Mar. 11, 2011, there happened a 9.0 degree earthquake near Japan. Aside from the great damage from earthquake and tsunami, the ensuing nuclear leakage raised more risks to the people and their country. Although human beings have Have been through the horrible nightmare of Chernobyl Disaster, we are still incapable to solve this kind of problem effectively and safely.</p><br />
<p>&nbsp;&nbsp;&nbsp;&nbsp;With constant improvement of synthetic biology, it lends possibility to utilize engineered organisms to implement the dangerous mission, clearance of nuclear leakage. Then, the question is, how to design a coherent system to achieve the goal?</p><br />
<p>&nbsp;</p><br />
<br />
<h6>2. Idea</h6><br />
<p>&nbsp;&nbsp;&nbsp;&nbsp;To approach our objective, there are two basic questions to be answered. First, is there any feasible way to control an organism to search the nuclear leakage in the radioactive environment? Second, is there any way to absorb the main substances of nuclear leakage?</p><br />
<br />
<p>&nbsp;&nbsp;&nbsp;&nbsp;In the next several weeks after we have determined our goal, we have found several important researches. First, there are two promoters, recA and recN in E.Coli which could be started when E.Coli is exposed to irradiation. With these promoters, the expression of key protein CheZ in chemotaxis of E.Coli could be controlled so that the movement of E.Coli can be instructed towards radiant probably. Second, among several main radioactive elements in nuclear leakage, iodine-131 and radon-222 have short half-life (several days) but long harmful influence on human bodies, while cesium-137 has long half-life (about 30 years) in environment. Although we found the protein which could absorb iodine first, it is so enormous and complex for E.Coli to assemble. However, the transmembrane protein trkD in E.Coli has low absorption to cesium, which gives us hope to dispose one of the great harmful elements in the leakage. At last, we realize the constitutive RFP and GFP could help measuring the intensity of radiation and Antigen43 has the function to gather all engineered E.Coli.</p><br />
<br />
<p>&nbsp;&nbsp;&nbsp;&nbsp;With above paramount parts, the design of nuclear-leakage rescuer has come out.</p><br />
<p>&nbsp;</p><br />
<br />
<h6>3. Elements</h6><br />
<p>&nbsp;&nbsp;&nbsp;&nbsp;1) Promoters</p><br />
<p>&nbsp;&nbsp;&nbsp;&nbsp;Gene recA and recN in most bacterial have reflection to the irradiation and recNp has a higher threshold compared with recAp. Both promoters are responsible to repair of DNA damage, which is one of consequences when bacterial are exposed to the radiation. The small pieces of DNA could be combined by RecA or RecN, and then both proteins have the activity of co-enzymes, which help repression proteion LexA to self-degrade. Consequently, promoters recAp and recNp are free from LexA, resulting in more expression of RecA and RecN, which is called the SOS System. Relatively, recAp responses to 2 Gy's (intensity of radiation) irradiation and recNp responses to 10 Gy's irradiation [1]. Because of the different thresholds of promoters, we could guide E.Coli to find the nuclear substances first and then implement the absorption. In this case, the system would be more stable and effective.</p><br />
<br />
<p>&nbsp;&nbsp;&nbsp;&nbsp;2) Proteins</p><br />
<p>&nbsp;&nbsp;&nbsp;&nbsp;Protein CheZ is a cytosolic phosphatase which functions in the chemotaxis signal transduction complex, another of which is CheY. The direction of rotation of the flagellar motor is controlled by the protein CheY. When CheY is not phosphorylated, the flagellar motor rotates counterclockwise (CCW), resulting in moving ahead of E.Coli. (Figure 2) When CheY is phosphorylated (CheY-P), it can bind to the flagellar motor protein FliM, causing the cell to tumble [2]. If we can control the expression of CheZ effectively, it is possible to control the movement of E.Coli.</p><br />
<br />
<p>&nbsp;&nbsp;&nbsp;&nbsp;Figure 1 The pathway of interaction in chemotaxis<br />
(Picture is from Shana Topp and Justin P. Gallivan, J. AM. CHEM. SOC. 2007, 129, 6807 6811)</p><br />
<br />
<p>&nbsp;&nbsp;&nbsp;&nbsp;Protein TrkD (belongs to Kup system) is responsible for the low-affinity transport of potassium into the cell, with the probable high affinity to transport cesium. In contrast to Trk or Kdp, the Kup system does not strongly distinguish between the alkali cations K+, Rb+, or even Cs+. When both of Cs+ and K+ exist, the uptake of Cs+ has inhibition of that of K+ [3]. And among several proteins in Kup system, TrkD has the best effect of uptake of Cs+. Accordingly, when E.Coli approached Cs+, the expression of TrkD would be modulated to a high level to absorb Cs-137.</p><br />
<br />
<p>&nbsp;&nbsp;&nbsp;&nbsp;Protein Antigen43 is a unique autotransporter that promotes bacterial cell-to-cell aggregation. Antigen 43 can be expressed on the E.coli cell surface in large quantities, up to 50,000 copies per cell [4]. After a period of absorption, the high expression of Antigen43 would lead to the aggregation of the colony.</p><br />
<br />
<p>&nbsp;&nbsp;&nbsp;&nbsp;3) Plasmids</p><br />
<p>&nbsp;&nbsp;&nbsp;&nbsp;Considering the manipulation of function, we use low-copy plasmids to express relatively few CheZ, in case of the long time of degradation impedes the control of the movement. On the other side, we use high expression plasmid to produce TrkD, so that the efficiency of absorption could be guaranteed.</p><br />
<br />
<p>&nbsp;&nbsp;&nbsp;&nbsp;4) Vectors</p><br />
<p>&nbsp;&nbsp;&nbsp;&nbsp;To reduce the background of result, we use strains with cheZ knocking out, so that the consequence would not be disturbed by self-product. Although trkD should be knocked out from the genome of the strain too, it doesn't affect the effect of absorption of cesium ion.</p><br />
<p>&nbsp;</p><br />
<br />
<h6>4. System</h6><br />
<p>&nbsp;&nbsp;&nbsp;&nbsp;1) Chemotaxis towards irradiation</p><br />
<p>&nbsp;&nbsp;&nbsp;&nbsp;We construct a part consist of recAp , cheZ, egfp, rfp. Under irradiation, recAp can be started to promote the expression of CheZ. With CheZ, the direction of rotation of the flagellar motor would be counterclockwise and E. Coli would move forward. However, without irradiation or E. Coli moves into a normal environment, the quantity of CheZ would decrease dramatically, leading the direction of rotation of the flagellar motor to be clockwise, then the cell would tumble in one location. Meanwhile, expression of EGFP and RFP has positive correlation to expression of CheZ. In some conditions, we can see that the cells are green under normal light, which helps us to recognize whether the promoters are started more easily.</p><br />
<br />
<p>&nbsp;&nbsp;&nbsp;&nbsp;2) Measurement of intensity of radiation</p><br />
<p>&nbsp;&nbsp;&nbsp;&nbsp;When the EGFP is expressed, RFP is constitutive expressed at the same. Different cells usually have different backgrounds under fluorescence microscope. With EGFP we could know whether there exists radiation, but we cannot measure the intensity of radiation. However, with constitutive expression of RFP, the relative value of EGFP to RFP must have correlation to the intensity of radiation. In other word, RFP helps to delete the difference in background, which can be used to measure the intensity of radiation.</p><br />
<br />
<p>&nbsp;&nbsp;&nbsp;&nbsp;3) Absorption of cesium</p><br />
<p>&nbsp;&nbsp;&nbsp;&nbsp;We construct a part consist of recNp, trkD and egfp. When E. Coli approaches radioactive elements, the intensity of radiation is much more than distant areas. After the intensity is higher than the threshold of recNp, the high expression of TrkD would be started. As a result, E. Coli would absorb cesium-137 around it.</p><br />
<br />
<p>&nbsp;&nbsp;&nbsp;&nbsp;4) Aggregation and collection</p><br />
<p>&nbsp;&nbsp;&nbsp;&nbsp;Despite of success of absorption of cesium-137 in the environment, the radioactive elements are still there. So we need to find a way to clear the leakage more easily. At this time, Antigen 43 helps to aggregate the cells together tightly and the relatively big mass is easier for people to collect. Especially in the sea area, the mass would subside to the bottom more quickly and the risk distant from the mass would be much less serious. We plan to construct this part on the same plasmid with trkD, but there is a time-delay device to postpone the expression of Antigen43, so that E. Coli has enough time to complete the task of absorption.</p><br />
<br />
<h6>5. Whole appearance of rescuers</h6><br />
<p>&nbsp;&nbsp;&nbsp;&nbsp;With the parts above, an E. Coli could sense the irradiation and move to radioactive areas. When E. Coli approaches the irradiation elements, the intensity of radiation would be higher. Then TrkD would be expressed highly to absorb cesium-137 because of the start of recNp. After absorption for a period of time, Antigen43 would be expressed highly and all E. Coli would gather into a ball in the water finally.</p><br />
<br />
<h6>6. Future work</h6><br />
<p>&nbsp;&nbsp;&nbsp;&nbsp;After about 3-months' efforts, we have constructed the chemotactic part and absorption part successfully. However, we still have a lot of work to do to integrate our project. For instance, we need to construct the aggregating part and measuring part later and adjust its parameters. Otherwise, the specificity and sensitivity of temporal parts need to be improved more, so that better manipulation would be achieved. Furthermore, with consideration about safety of engineered E. Coli inputted into our environment, we will design a proper suicide device to protect these functional plasmids from gene flow.</p><br />
<p>&nbsp;</p><br />
<br />
<br />
References<br />
<li>[1] S. Nuyts et al., Radiation Research. 155, 716 (2001).</li><br />
<li>[2] S. Topp, Justin P. Gallivan, J. AM. CHEM. SOC. 129, 6807 (2003).</li><br />
<li>[3] D. Bossemeyer et al., Journal of Bacteriology. 171, 2219 (1989).</li><br />
<li>[4] P. Marguet et al., JR Soc Interface. 4, 607 (2007).</li><br />
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<li> <a href="https://static.igem.org/mediawiki/2011/1/13/Story_Figure_1.jpg" title=""><img src="https://static.igem.org/mediawiki/2011/a/a2/Story_Figure_1_s.jpg" width="150" height="200" /></a> <h1>Figure 1</h1><br />
<p>The explosion of Fukushima nuclear power station</p></li><br />
<br /><br /> <br /><br /> <br /><br /> <br /><br /> <br /><br /> <br /><br /> <br /><br /> <br /><br /> <br /><br /> <br /><br /> <br /><br /> <br /><br /> <br /><br /> <br /><br /> <br /><br /> <br /><br /> <br /><br /> <br /><br /> <br /><br /> <br /><br /> <br /><br /> <br /><br /> <br /><br /> <br /><br /> <br /><br /> <br /><br /><br />
<li> <a href="https://static.igem.org/mediawiki/2011/7/7f/Story_Figure_2.jpg" title=""><img src="https://static.igem.org/mediawiki/2011/2/2a/Story_Figure_2s.jpg" width="150" height="63" /></a><h1>Figure 2</h1> </li><br />
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<li> <a href="https://static.igem.org/mediawiki/2011/2/2c/Story_Figure_3.jpg" title=""><img src="https://static.igem.org/mediawiki/2011/8/8c/Story_Figure_3_s.jpg" width="150" height="200" alt="" /></a><br />
<h1>Figure 3</h1><br />
<p>The transformed E.colis sense the radiation</p></li><br />
<br /> <br /><br /> <br /><br /> <br /><br /> <br /><br /> <br /><br /> <br /><br /> <br /><br />
<li> <a href="https://static.igem.org/mediawiki/2011/3/3e/Story_Figure_4.jpg" title=""><img src="https://static.igem.org/mediawiki/2011/2/2b/Story_Figure_4_s.jpg" width="150" height="200" alt="" /></a><br />
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<p>The E.coli is adsorbing the Cs elements</p></li><br />
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<h2>Pages we think helpful:</h2><br />
<h1><a href="http://www.guardian.co.uk/world/2011/mar/12/japan-earthquake-tsunami-aftermath-live">Japan nuclear alert</a></h1><br />
<h1><a href="http://www.world-nuclear.org/info/chernobyl/inf07.html<br />
">Chernobyl disaster</a></h1><br />
<h1><a href="http://img2.allvoices.com/thumbs/image/609/480/76513300-there-has.jpg">Stop of nuclear</a></h1><br />
<h1><a href="http://en.wikipedia.org/wiki/Chemotaxis">Chemotaxis - wikipedia</a></h1><br />
<h1><a href="http://www.itsyourhealth2.com/pages/products/barleylifevsbarleymax.htm">Cesium-137 in American</a></h1><br />
<h1><a href="http://schools.wikia.com/wiki/Radioactive_Decay">Radioactive Decay</a></h1><br />
<h1><a href="http://partsregistry.org/Part:BBa_K346007">Antigen43</a></h1><br />
<h1><a href="http://upload.wikimedia.org/wikipedia/commons/thumb/e/e4/GFP_structure.png/200px-GFP_structure.png">EGFP</a></h1><br />
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<p>Address: 135# Xingang Rd.(W.), Haizhu Guangzhou, P.R.China</p><br />
<p><a href="http://eng.sysu.edu.cn/">visit the Sun Yat-sen university website</a></p><br />
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<li><a href="https://2011.igem.org/Team:SYSU-China/page_aboutus_team_members"><span>Team Members</span></a><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/page_aboutus_special_thanks"><span>Spcial Thanks</span></a></li><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/page_aboutus_LT"><span>Logo and T-shirt</span></a></li><br />
</ul><br />
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</li><br />
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<div id="page_content_baBar_details"><a href="#page_content_textBar_enterTEXT">See Details</a></div><br />
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<br />
<ul><br />
<li><img name="" src="https://static.igem.org/mediawiki/2011/6/62/SYSU_Other_head.png" width="114" height="152" alt="" /><br />
<h1>Jian REN</h1><br />
<p>Sofeware Adviser</p><br />
</li><br />
<li><img name="" src="https://static.igem.org/mediawiki/2011/6/62/SYSU_Other_head.png" width="114" height="152" alt="" /><br />
<h1>XiongLei HE</h1><br />
<p>Adviser</p><br />
</li><br />
<li><img name="" src="https://static.igem.org/mediawiki/2011/6/62/SYSU_Other_head.png" width="114" height="152" alt="" /><br />
<h1>YongJun LU</h1><br />
<p>Adviser</p><br />
</li><br />
</ul><br />
<br />
<ul> <br />
<li><img name="" src="https://static.igem.org/mediawiki/2011/6/62/SYSU_Other_head.png" width="114" height="152" alt="" /><br />
<h1>Chao ZHANG</h1><br />
<p>Ex-member</p><br />
</li><br />
<li><img name="" src="https://static.igem.org/mediawiki/2011/6/62/SYSU_Other_head.png" width="114" height="152" alt="" /><br />
<h1>JiaYan ZHOU</h1><br />
<p>Animation Narrator</p><br />
</li><br />
<li><img name="" src="https://static.igem.org/mediawiki/2011/6/62/SYSU_Other_head.png" width="114" height="152" alt="" /><br />
<h1>YanQiu YANG</h1><br />
<p>Animation Producer</p><br />
</li><br />
<li><img name="" src="https://static.igem.org/mediawiki/2011/6/62/SYSU_Other_head.png" width="114" height="152" alt="" /><br />
<h1>Yi YANG</h1><br />
<p>A really cute girl& Ex-member</p><br />
</li><br />
<li><img name="" src="https://static.igem.org/mediawiki/2011/6/62/SYSU_Other_head.png" width="114" height="152" alt="" /><br />
<h1>ZeDa ZHANG</h1><br />
<p>Ex-member</p><br />
<p>Best friend of Wiki Coder ^ ^</p><br />
<p>God bless you</p><br />
</li><br />
</ul><br />
<ul><br />
<li id="fuking_tired"><br />
<h1>BinBin ZHENG</h1><br />
<p>&nbsp;&nbsp;&nbsp;&nbsp;Young enthusiastic biosynthetic student</p></li><br />
<li id="fuking_tired"><br />
<h1>PengHui YUAN</h1><br />
<p>&nbsp;&nbsp;&nbsp;&nbsp;Young enthusiastic biosynthetic student</p></li><br />
<li id="fuking_tired"><br />
<h1>Xia'Nan FU</h1><br />
<p>&nbsp;&nbsp;&nbsp;&nbsp;Young enthusiastic biosynthetic student</p></li><br />
<li id="fuking_tired"><br />
<h1>YunHao SUN</h1><br />
<p>&nbsp;&nbsp;&nbsp;&nbsp;Thank you Bro. Sui, for the hapiness you have brought to us</p></li><br />
<li id="fuking_tired"><br /><br /><br /></li> <br />
<li id="fuking_tired"><p>etc. We thank you all for helping us during the experiments and the making of the whole project.</p></li><br />
<br />
</ul> <br />
</div> <br />
</div><br />
</div><br />
<!---------------------------------------------!--><br />
<div class="page_content_RsideBar"><br />
<div class="page_content_relatedBar_aboutus"><br />
<h2>Pages we think helpful:</h2><br />
<h1><a href="https://2011.igem.org/Team:SYSU-China/page_aboutus_team_members">our team member page</a></h1><br />
<h1><a href="https://2011.igem.org/Team:SYSU-China/main_page_human_practice">our human practice page</a></h1><br />
<p>&nbsp;</p><br />
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<p>From the 2011 iGEM team SYSU-China (2011)</p><br />
<p>Sun Yat-Sen University, Guangzhou, China</p><br />
<p>Address: 135# Xingang Rd.(W.), Haizhu Guangzhou, P.R.China</p><br />
<p><a href="http://eng.sysu.edu.cn/">visit the Sun Yat-sen university website</a></p><br />
<p>Thanks Apycom jQuery Menus and <a href="http://apycom.com/">visit their website</a><br />
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</html></div>Cambihttp://2011.igem.org/File:SYSU_Other_head.pngFile:SYSU Other head.png2011-10-06T03:28:51Z<p>Cambi: uploaded a new version of &quot;File:SYSU Other head.png&quot;</p>
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<div></div>Cambihttp://2011.igem.org/Team:SYSU-China/page_project_modules_verificationTeam:SYSU-China/page project modules verification2011-10-06T03:13:57Z<p>Cambi: </p>
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<!--------here begins the manu bar section--!--><br />
<div class="title_container"><br />
<div id="menu"><br />
<ul class="menu"><br />
<li><a href="index.thml"><span></span></a></li><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/main_page_news"><span>NEWS</span></a></li><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/main_page_story"><span>STORY</span></a></li> <br />
<li><a href="https://2011.igem.org/Team:SYSU-China/main_page_project" class="parent"><span>PROJECT</span></a><br />
<div><br />
<ul><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/page_project_modules_construction"><span>Modules Construction</span></a><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/page_project_modules_verification"><span>Modules Verification</span></a></li><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/page_project_functional_construction"><span>Functional Verification</span></a></li><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/page_project_notes"><span>Notes</span></a></li><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/page_project_datapage"><span>Date Page</span></a></li><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/page_project_safety"><span>Safety</span></a></li><br />
</ul><br />
</div><br />
</li><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/main_page_human_practice" class="parent"><span>HUMAN PRACTICE</span></a><br />
<div><br />
<ul><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/page_human_practice_app"><span>App</span></a><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/page_human_practice_labcraft"><span>LabCraft Board Game</span></a></li><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/page_human_practice_workshop"><span>Workshop</span></a></li><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/page_human_practice_survey"><span>Survey</span></a></li><br />
</ul><br />
</div><br />
</li><br />
<br />
<li><a href="https://2011.igem.org/Team:SYSU-China/main_page_aboutus" class="parent"><span>ABOUT US</span></a><br />
<div><br />
<ul><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/page_aboutus_team_members"><span>Team Members</span></a><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/page_aboutus_special_thanks"><span>Spcial Thanks</span></a></li><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/page_aboutus_LT"><span>Logo and T-shirt</span></a></li><br />
</ul><br />
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</li><br />
<br />
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<h2>Fluorescence </h2><br />
<div class="page_content_textBar_enterTEXT_enter"><br />
<br /><br />
<p>&nbsp;&nbsp;&nbsp;&nbsp;We decided to examine the expression of the two promoters- PrecA and PrecN, and two genes-cheZ and trkD, via the fluorescent intensity of EGFP whose sequence had been ligated downstream after them. </p><br />
<p>&nbsp;&nbsp;&nbsp;&nbsp;We have constructed a plasmid (pUC18) with EGFP, which is ligated to the plasmid in the PstI and HindIII restriction enzyme cutting sites. Then we link PrecA,PrecN,cheZ,trkD gene to EGFP sequence respectively to construct four plasmids, recA-GFP-pUC18,recN-GFP-pUC18,cheZ-GFP-pUC18 and trkD-GFP-pUC18. We used ultraviolet or nalidixic acid to damage E.coli's DNA to trigger the expression of PrecA and PrecN on in order to examine the expression of EGFP. However, there is a lac promoter upstream before the multi-cloning site of pUC18, which allows us to use IPTG induction to examine the expression of cheZ and trkD by watching the GFP expression. In order to ensure the expression of two genes and GFP, we deleted the terminator of the two genes, cheZ and trkD.</p><br />
<p>&nbsp;&nbsp;&nbsp;&nbsp;Using the laser scanning confocal microscope (LSCM), we can see obvious fluorescence of cheZ-GFP-pUC18 and trkD-GFP-pUC18(Figure 1 and Figure 2). The fluorescence of trkD-GFP-pUC18, is located mostly on the membrane of E.coli. The lac promoter, however, will express without induction of IPTG, resulting in the expression of GFP of control group, which is cheZ-GFP-pUC18 and trkD-GFP-pUC18 that are not induced by IPTG. Consequently, we utilized the Flow Cytometer(FCM) to exam the intensity of GFP in control group and experiment group. (We are still working on that)</p><br />
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<ul><br />
<li> <a href="https://static.igem.org/mediawiki/2011/3/3b/SYSU_1_chez-GFP-pUC18-control.jpg" title=""><img src="https://static.igem.org/mediawiki/igem.org/8/87/1_chez-GFP-pUC18-control_S.jpg" width="150" height="126" alt="Flower" /></a> <p>chez-GFP-pUC18-control</p></li><br />
<li> <a href="https://static.igem.org/mediawiki/2011/8/83/SYSU_2_chez-GFP-pUC18-experiment.jpg" title=""><img src="https://static.igem.org/mediawiki/igem.org/4/43/2_chez-GFP-pUC18-experiment_S.jpg" width="150" height="126" alt="Tree" /></a><p>chez-GFP-pUC18-experiment</p></li><br />
<li> <a href="https://static.igem.org/mediawiki/2011/e/e1/SYSU_3_trkD-GFP-pUC18-control.jpg" title=""><img src="https://static.igem.org/mediawiki/igem.org/d/d1/3_trkD-GFP-pUC18-control_S.jpg" width="150" height="126" alt="" /></a><p>trkD-GFP-pUC18-control</p> </li><br />
<li> <a href="https://static.igem.org/mediawiki/2011/d/d8/SYSU_4_trkd-GFP-pUC18-experiment.jpg" title=""><img src="https://static.igem.org/mediawiki/igem.org/8/81/4_trkd-GFP-pUC18-experiment_S.jpg" width="150" height="126" alt="" /></a><p>trkd-GFP-pUC18-experiment</p></li><br />
<li> <a href="https://static.igem.org/mediawiki/2011/a/a8/SYSU_5_trkD-GFP-pUC18-control1.jpg" title=""><img src="https://static.igem.org/mediawiki/igem.org/3/36/5_trkD-GFP-pUC18-control1_S.jpg" width="150" height="126" alt="" /></a><p>trkD-GFP-pUC18-control</li><br />
<li> <a href="https://static.igem.org/mediawiki/2011/0/0f/SYSU_6_trkd-GFP-pUC18-experiment1.jpg" title=""><img src="https://static.igem.org/mediawiki/igem.org/8/84/6_trkd-GFP-pUC18-experiment1_S.jpg" width="150" height="126" alt="" /></a><p>trkd-GFP-pUC18-experiment</p></li><br />
</ul><br />
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<h2>Western Blot test</h2><br />
<br /><br />
<div class="page_content_textBar_enterTEXT_enter"><p>&nbsp;&nbsp;&nbsp;&nbsp;We planned to exame the expression of gene cheZ and trkD on protein level. Within the inducement of 0.1mg/mL Isopropyl β-D-1-thiogalactopyranoside(IPTG) at 18℃ for 15~18h, we extracted the total proteins of the E.coli transformed with CheZ-pET28a or TrkD-pET32a. Western Blot results showed that the quantity of protein CheZ and TrkD significantly increased after the inducement, indicating that cheZ and trkD expressed well. Additionally, sicne a basal expression caused by promoter lac in both plasmids, the control groups showed a small quantity of proteins. </p></div><br />
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<li> <a href="https://static.igem.org/mediawiki/igem.org/9/92/Western_blot_L.png" title=""><img src="https://static.igem.org/mediawiki/igem.org/a/ae/Westerblot_s.png" width="150" height="137" alt="Flower" /></a> </li><br />
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<h2>Cesium absorption test </h2><br />
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<p>&nbsp;&nbsp;&nbsp;&nbsp;We use TrkD-pET32a to test the function of trkD. The test could be accomplished through the detection of the amount of Cs+ absorption in the experiment group and control group. </p><br />
<p>&nbsp;&nbsp;&nbsp;&nbsp;The analysis of Cs+ absorption through trkD indicates that under the inducement of IPTG (0.5mM) for about 4h, the trkD constructed in plasmid can express normally, thus the experiment group has a higher amount of absorption than that of the control group. While the reason why the control has also absorbed Cs+ is that gene trkD also exists in E.coli's genome, so the control group can also express a certain amount of trkD to accomplish the absorption of Cs+. As a result, the absorption of the experiment group is 47.8% higher than that of the control group.</p><br />
<p>&nbsp;&nbsp;&nbsp;&nbsp;In terms of the result, we have proved that the trkD constructed in the plasmid can function normally. </p><br />
</div><br />
<h2>Characterizing Promoters</h2><br />
<br /><br />
<div class="page_content_textBar_enterTEXT_enter"> <P>&nbsp;&nbsp;&nbsp;&nbsp;Because we want to characterize recA and recN promoters, we turned to Microplate Reader, a machine that can measure the overall fluorescent intensity. We set the exciting wave at 488nm and the emission wave at 520nm, which allow us the measure the fluorescence of recA-GFP-pET28a-BL21 and recA-GFP-pET28a-BL21. After induce with NAL and regulate the OD value of each inocula to a same value, we measured the fluorescence intensity of recA-GFP-pET28a-BL21 and recA-GFP-pET28a-BL21.</P><br />
<p>&nbsp;&nbsp;&nbsp;&nbsp;About the two crests of the curve of recA-GFP-pET28a-BL21, we guess that the rec promoter inside the genome of the BL21 itself, beside the one caused by rec promoter in the plasmid, may be the reason of another crest. At first, the recA promoter is triggered by the increasing NAL concentration, which leads to the first crest. As the NAL concentration increases, the DNA of the plasmid was damaged but the rec promoter in the genome has not been triggered yet since the concentration of the NAL has not reached its threshold, which results in the trough between the two crests. Finally, the rec promoter in the genome is triggered by the relatively high concentration of NAL and produces the protein of rec family, which may help repair the DNA of the plasmid, which causes the second crest of the curve.</p><br />
<p>&nbsp;&nbsp;&nbsp;&nbsp;The curve of recN-GFP-pET28a-BL21 seems to support our hypothesis. The curve of recN-GFP-pET28a-BL21 has a platform instead of a trough. Then a crest after that. We guess that the threshold of recN promoter is higher than recA promoter is the reason. The crest caused by the recN promoter in the plasmid overlappes with the trough, which creates a platform as we see.</p><br />
<p>&nbsp;&nbsp;&nbsp;&nbsp;As the why the the rec promoter of the plasmid will be damaged earlier than that in the genome, we guess the different position of the two promoters is the reason.</p><br />
<p>&nbsp;&nbsp;&nbsp;&nbsp;However, the difference between all the data is not convincing enough, which may lead to another explanation: all the tendency of the data is caused by system error, and yet, which we haven't found out. </p><br />
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<li> <a href="https://static.igem.org/mediawiki/igem.org/f/f3/1.jpg" title=""><img src="https://static.igem.org/mediawiki/igem.org/8/82/Mb1S.png" width="150" height="88" alt="Flower" /></a> <br />
<p>Figure1.&nbsp;&nbsp;&nbsp;&nbsp;Fluorescence intensity of recA-GFP-pET28a-BL21 in the first and second experiments</p><br />
</li><br />
<li> <a href="https://static.igem.org/mediawiki/igem.org/0/0b/Mb2.png" title=""><img src="https://static.igem.org/mediawiki/igem.org/4/41/Mb2S.png" width="150" height="92" alt="Flower" /></a> <p>Figure2.&nbsp;&nbsp;&nbsp;&nbsp;Fluorescence intensity of recA-GFP-pET28a-BL21 in the third and forth experiments</p><br />
</li><br />
<li> <a href="https://static.igem.org/mediawiki/igem.org/c/c5/Mb3.png" title=""><img src="https://static.igem.org/mediawiki/igem.org/d/df/Mb3S.png" width="150" height="99" alt="Flower" /></a> <p>Figure3.&nbsp;&nbsp;&nbsp;&nbsp;Fluorescence intensity of recN-GFP-pET28a-BL21 in all the five experiments</p> </li><br />
</ul><br />
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<h2>Pages we think helpful:</h2><br />
<h1><a href="https://2011.igem.org/Team:SYSU-China/page_project_modules_construction">see how we construct our modules</a></h1><br />
<h1><a href="https://2011.igem.org/Team:SYSU-China/page_project_modules_verification">see how we verify each module</a></h1><br />
<h1><a href="https://2011.igem.org/Team:SYSU-China/main_page_story">or see our whole idea in Story page</a></h1><br />
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<p>From the 2011 iGEM team SYSU-China (2011)</p><br />
<p>Sun Yat-Sen University, Guangzhou, China</p><br />
<p>Address: 135# Xingang Rd.(W.), Haizhu Guangzhou, P.R.China</p><br />
<p><a href="http://eng.sysu.edu.cn/">visit the Sun Yat-sen university website</a></p><br />
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</html></div>Cambihttp://2011.igem.org/Team:SYSU-China/page_project_modules_verificationTeam:SYSU-China/page project modules verification2011-10-06T03:13:22Z<p>Cambi: </p>
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<li><a href="https://2011.igem.org/Team:SYSU-China/page_aboutus_team_members"><span>Team Members</span></a><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/page_aboutus_special_thanks"><span>Spcial Thanks</span></a></li><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/page_aboutus_LT"><span>Logo and T-shirt</span></a></li><br />
</ul><br />
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<h2>Fluorescence </h2><br />
<div class="page_content_textBar_enterTEXT_enter"><br />
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<p>&nbsp;&nbsp;&nbsp;&nbsp;We decided to examine the expression of the two promoters- PrecA and PrecN, and two genes-cheZ and trkD, via the fluorescent intensity of EGFP whose sequence had been ligated downstream after them. </p><br />
<p>&nbsp;&nbsp;&nbsp;&nbsp;We have constructed a plasmid (pUC18) with EGFP, which is ligated to the plasmid in the PstI and HindIII restriction enzyme cutting sites. Then we link PrecA,PrecN,cheZ,trkD gene to EGFP sequence respectively to construct four plasmids, recA-GFP-pUC18,recN-GFP-pUC18,cheZ-GFP-pUC18 and trkD-GFP-pUC18. We used ultraviolet or nalidixic acid to damage E.coli's DNA to trigger the expression of PrecA and PrecN on in order to examine the expression of EGFP. However, there is a lac promoter upstream before the multi-cloning site of pUC18, which allows us to use IPTG induction to examine the expression of cheZ and trkD by watching the GFP expression. In order to ensure the expression of two genes and GFP, we deleted the terminator of the two genes, cheZ and trkD.</p><br />
<p>&nbsp;&nbsp;&nbsp;&nbsp;Using the laser scanning confocal microscope (LSCM), we can see obvious fluorescence of cheZ-GFP-pUC18 and trkD-GFP-pUC18(Figure 1 and Figure 2). The fluorescence of trkD-GFP-pUC18, is located mostly on the membrane of E.coli. The lac promoter, however, will express without induction of IPTG, resulting in the expression of GFP of control group, which is cheZ-GFP-pUC18 and trkD-GFP-pUC18 that are not induced by IPTG. Consequently, we utilized the Flow Cytometer(FCM) to exam the intensity of GFP in control group and experiment group. (We are still working on that)</p><br />
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<li> <a href="https://static.igem.org/mediawiki/2011/3/3b/SYSU_1_chez-GFP-pUC18-control.jpg" title=""><img src="https://static.igem.org/mediawiki/igem.org/8/87/1_chez-GFP-pUC18-control_S.jpg" width="150" height="126" alt="Flower" /></a> <p>chez-GFP-pUC18-control</p></li><br />
<li> <a href="https://static.igem.org/mediawiki/2011/8/83/SYSU_2_chez-GFP-pUC18-experiment.jpg" title=""><img src="https://static.igem.org/mediawiki/igem.org/4/43/2_chez-GFP-pUC18-experiment_S.jpg" width="150" height="126" alt="Tree" /></a><p>chez-GFP-pUC18-experiment</p></li><br />
<li> <a href="https://static.igem.org/mediawiki/2011/e/e1/SYSU_3_trkD-GFP-pUC18-control.jpg" title=""><img src="https://static.igem.org/mediawiki/igem.org/d/d1/3_trkD-GFP-pUC18-control_S.jpg" width="150" height="126" alt="" /></a><p>trkD-GFP-pUC18-control</p> </li><br />
<li> <a href="https://static.igem.org/mediawiki/2011/d/d8/SYSU_4_trkd-GFP-pUC18-experiment.jpg" title=""><img src="https://static.igem.org/mediawiki/igem.org/8/81/4_trkd-GFP-pUC18-experiment_S.jpg" width="150" height="126" alt="" /></a><p>trkd-GFP-pUC18-experiment</p></li><br />
<li> <a href="https://static.igem.org/mediawiki/2011/a/a8/SYSU_5_trkD-GFP-pUC18-control1.jpg" title=""><img src="https://static.igem.org/mediawiki/igem.org/3/36/5_trkD-GFP-pUC18-control1_S.jpg" width="150" height="126" alt="" /></a><p>trkD-GFP-pUC18-control</li><br />
<li> <a href="https://static.igem.org/mediawiki/2011/0/0f/SYSU_6_trkd-GFP-pUC18-experiment1.jpg" title=""><img src="https://static.igem.org/mediawiki/igem.org/8/84/6_trkd-GFP-pUC18-experiment1_S.jpg" width="150" height="126" alt="" /></a><p>trkd-GFP-pUC18-experiment</p></li><br />
</ul><br />
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<h2>Western Blot test</h2><br />
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<div class="page_content_textBar_enterTEXT_enter"><p>&nbsp;&nbsp;&nbsp;&nbsp;We planned to exame the expression of gene cheZ and trkD on protein level. Within the inducement of 0.1mg/mL Isopropyl β-D-1-thiogalactopyranoside(IPTG) at 18℃ for 15~18h, we extracted the total proteins of the E.coli transformed with CheZ-pET28a or TrkD-pET32a. Western Blot results showed that the quantity of protein CheZ and TrkD significantly increased after the inducement, indicating that cheZ and trkD expressed well. Additionally, sicne a basal expression caused by promoter lac in both plasmids, the control groups showed a small quantity of proteins. </p></div><br />
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<ul><br />
<li> <a href="https://static.igem.org/mediawiki/igem.org/9/92/Western_blot_L.png" title=""><img src="https://static.igem.org/mediawiki/igem.org/a/ae/Westerblot_s.png" width="150" height="137" alt="Flower" /></a> </li><br />
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<h2>Cesium absorption test </h2><br />
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<div class="page_content_textBar_enterTEXT_enter"><br />
<p>&nbsp;&nbsp;&nbsp;&nbsp;We use TrkD-pET32a to test the function of trkD. The test could be accomplished through the detection of the amount of Cs+ absorption in the experiment group and control group. </p><br />
<p>&nbsp;&nbsp;&nbsp;&nbsp;The analysis of Cs+ absorption through trkD indicates that under the inducement of IPTG (0.5mM) for about 4h, the trkD constructed in plasmid can express normally, thus the experiment group has a higher amount of absorption than that of the control group. While the reason why the control has also absorbed Cs+ is that gene trkD also exists in E.coli's genome, so the control group can also express a certain amount of trkD to accomplish the absorption of Cs+. As a result, the absorption of the experiment group is 47.8% higher than that of the control group.</p><br />
<p>&nbsp;&nbsp;&nbsp;&nbsp;In terms of the result, we have proved that the trkD constructed in the plasmid can function normally. </p><br />
</div><br />
<h2>Characterizing Promoters</h2><br />
<br /><br />
<div class="page_content_textBar_enterTEXT_enter"> <P>&nbsp;&nbsp;&nbsp;&nbsp;Because we want to characterize recA and recN promoters, we turned to Microplate Reader, a machine that can measure the overall fluorescent intensity. We set the exciting wave at 488nm and the emission wave at 520nm, which allow us the measure the fluorescence of recA-GFP-pET28a-BL21 and recA-GFP-pET28a-BL21. After induce with NAL and regulate the OD value of each inocula to a same value, we measured the fluorescence intensity of recA-GFP-pET28a-BL21 and recA-GFP-pET28a-BL21.</P><br />
<p>&nbsp;&nbsp;&nbsp;&nbsp;About the two crests of the curve of recA-GFP-pET28a-BL21, we guess that the rec promoter inside the genome of the BL21 itself, beside the one caused by rec promoter in the plasmid, may be the reason of another crest. At first, the recA promoter is triggered by the increasing NAL concentration, which leads to the first crest. As the NAL concentration increases, the DNA of the plasmid was damaged but the rec promoter in the genome has not been triggered yet since the concentration of the NAL has not reached its threshold, which results in the trough between the two crests. Finally, the rec promoter in the genome is triggered by the relatively high concentration of NAL and produces the protein of rec family, which may help repair the DNA of the plasmid, which causes the second crest of the curve.</p><br />
<p>&nbsp;&nbsp;&nbsp;&nbsp;The curve of recN-GFP-pET28a-BL21 seems to support our hypothesis. The curve of recN-GFP-pET28a-BL21 has a platform instead of a trough. Then a crest after that. We guess that the threshold of recN promoter is higher than recA promoter is the reason. The crest caused by the recN promoter in the plasmid overlappes with the trough, which creates a platform as we see.</p><br />
<p>&nbsp;&nbsp;&nbsp;&nbsp;As the why the the rec promoter of the plasmid will be damaged earlier than that in the genome, we guess the different position of the two promoters is the reason.</p><br />
<p>&nbsp;&nbsp;&nbsp;&nbsp;However, the difference between all the data is not convincing enough, which may lead to another explanation: all the tendency of the data is caused by system error, and yet, which we haven't found out. </p><br />
</div><br />
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<ul><br />
<li> <a href="https://static.igem.org/mediawiki/igem.org/f/f3/1.jpg" title=""><img src="https://static.igem.org/mediawiki/igem.org/8/82/Mb1S.png" width="150" height="88" alt="Flower" /></a> <br />
<p>Figure1.&nbsp;&nbsp;&nbsp;&nbsp;Fluorescence intensity of recA-GFP-pET28a-BL21 in the first and second experiments</p><br />
</li><br />
<li> <a href="https://static.igem.org/mediawiki/igem.org/0/0b/Mb2.png" title=""><img src="https://static.igem.org/mediawiki/igem.org/4/41/Mb2S.png" width="150" height="92" alt="Flower" /></a> <p>Figure2.&nbsp;&nbsp;&nbsp;&nbsp;Fluorescence intensity of recA-GFP-pET28a-BL21 in the third and forth experiments</p><br />
</li><br />
<li> <a href="https://static.igem.org/mediawiki/igem.org/c/c5/Mb3.png" title=""><img src="https://static.igem.org/mediawiki/igem.org/d/df/Mb3S.png" width="150" height="99" alt="Flower" /></a> <p>Figure3.&nbsp;&nbsp;&nbsp;&nbsp;Fluorescence intensity of recN-GFP-pET28a-BL21 in all the five experiments</p> </li><br />
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<h2>Pages we think helpful:</h2><br />
<h1><a href="https://2011.igem.org/Team:SYSU-China/page_project_modules_construction">see how we construct our modules</a></h1><br />
<h1><a href="https://2011.igem.org/Team:SYSU-China/page_project_modules_verification">see how we verify each module</a></h1><br />
<h1><a href="https://2011.igem.org/Team:SYSU-China/main_page_story">or see our whole idea in Story page</a></h1><br />
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<p>From the 2011 iGEM team SYSU-China (2011)</p><br />
<p>Sun Yat-Sen University, Guangzhou, China</p><br />
<p>Address: 135# Xingang Rd.(W.), Haizhu Guangzhou, P.R.China</p><br />
<p><a href="http://eng.sysu.edu.cn/">visit the Sun Yat-sen university website</a></p><br />
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<p>This is the Che protein family system we had used<br />
(Picture is from Shana Topp and Justin P. Gallivan, J. AM. CHEM. SOC. 2007, 129, 6807 6811)<br /><br />
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<p> <br />
<p>See SYSU-China 2011 iGEM Team Parts<br />
<p>&nbsp; </p><br />
<p><a href="http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2011&amp;group=SYSU-China">http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2011&amp;group=SYSU-China</a></p><br />
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<h2>Pages we think helpful:</h2><br />
<h1><a href="https://2011.igem.org/Team:SYSU-China/page_project_modules_construction">see how we construct our modules</a></h1><br />
<h1><a href="https://2011.igem.org/Team:SYSU-China/page_project_modules_verification">see how we verify each module</a></h1><br />
<h1><a href="https://2011.igem.org/Team:SYSU-China/main_page_story">or see our whole idea in Story page</a></h1><br />
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<!--here begins the contact_bottombar!--><br />
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<p>From the 2011 iGEM team SYSU-China (2011)</p><br />
<p>Sun Yat-Sen University, Guangzhou, China</p><br />
<p>Address: 135# Xingang Rd.(W.), Haizhu Guangzhou, P.R.China</p><br />
<p><a href="http://eng.sysu.edu.cn/">visit the Sun Yat-sen university website</a></p><br />
<p>Thanks Apycom jQuery Menus and <a href="http://apycom.com/">visit their website</a><br />
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<div id="page_content_baBar_details"><a href="#TabbedPanels2">See Details</a></div><br />
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<h2>Fluorescence </h2><br />
<div class="page_content_textBar_enterTEXT_enter"><br />
<br /><br />
<p>&nbsp;&nbsp;&nbsp;&nbsp;We decided to examine the expression of the two promoters- PrecA and PrecN, and two genes-cheZ and trkD, via the fluorescent intensity of EGFP whose sequence had been ligated downstream after them. </p><br />
<p>&nbsp;&nbsp;&nbsp;&nbsp;We have constructed a plasmid (pUC18) with EGFP, which is ligated to the plasmid in the PstI and HindIII restriction enzyme cutting sites. Then we link PrecA,PrecN,cheZ,trkD gene to EGFP sequence respectively to construct four plasmids, recA-GFP-pUC18,recN-GFP-pUC18,cheZ-GFP-pUC18 and trkD-GFP-pUC18. We used ultraviolet or nalidixic acid to damage E.coli's DNA to trigger the expression of PrecA and PrecN on in order to examine the expression of EGFP. However, there is a lac promoter upstream before the multi-cloning site of pUC18, which allows us to use IPTG induction to examine the expression of cheZ and trkD by watching the GFP expression. In order to ensure the expression of two genes and GFP, we deleted the terminator of the two genes, cheZ and trkD.</p><br />
<p>&nbsp;&nbsp;&nbsp;&nbsp;Using the laser scanning confocal microscope (LSCM), we can see obvious fluorescence of cheZ-GFP-pUC18 and trkD-GFP-pUC18(Figure 1 and Figure 2). The fluorescence of trkD-GFP-pUC18, is located mostly on the membrane of E.coli. The lac promoter, however, will express without induction of IPTG, resulting in the expression of GFP of control group, which is cheZ-GFP-pUC18 and trkD-GFP-pUC18 that are not induced by IPTG. Consequently, we utilized the Flow Cytometer(FCM) to exam the intensity of GFP in control group and experiment group. (We are still working on that)</p><br />
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<li> <a href="https://static.igem.org/mediawiki/2011/3/3b/SYSU_1_chez-GFP-pUC18-control.jpg" title=""><img src="https://static.igem.org/mediawiki/igem.org/8/87/1_chez-GFP-pUC18-control_S.jpg" width="150" height="126" alt="Flower" /></a> </li><br />
<li> <a href="https://static.igem.org/mediawiki/2011/8/83/SYSU_2_chez-GFP-pUC18-experiment.jpg" title=""><img src="https://static.igem.org/mediawiki/igem.org/4/43/2_chez-GFP-pUC18-experiment_S.jpg" width="150" height="126" alt="Tree" /></a> </li><br />
<li> <a href="https://static.igem.org/mediawiki/2011/e/e1/SYSU_3_trkD-GFP-pUC18-control.jpg" title=""><img src="https://static.igem.org/mediawiki/igem.org/d/d1/3_trkD-GFP-pUC18-control_S.jpg" width="150" height="126" alt="" /></a> </li><br />
<li> <a href="https://static.igem.org/mediawiki/2011/d/d8/SYSU_4_trkd-GFP-pUC18-experiment.jpg" title=""><img src="https://static.igem.org/mediawiki/igem.org/8/81/4_trkd-GFP-pUC18-experiment_S.jpg" width="150" height="126" alt="" /></a> </li><br />
<li> <a href="https://static.igem.org/mediawiki/2011/a/a8/SYSU_5_trkD-GFP-pUC18-control1.jpg" title=""><img src="https://static.igem.org/mediawiki/igem.org/3/36/5_trkD-GFP-pUC18-control1_S.jpg" width="150" height="126" alt="" /></a> </li><br />
<li> <a href="https://static.igem.org/mediawiki/2011/0/0f/SYSU_6_trkd-GFP-pUC18-experiment1.jpg" title=""><img src="https://static.igem.org/mediawiki/igem.org/8/84/6_trkd-GFP-pUC18-experiment1_S.jpg" width="150" height="126" alt="" /></a> </li><br />
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<h2>Western Blot test</h2><br />
<br /><br />
<div class="page_content_textBar_enterTEXT_enter"><p>&nbsp;&nbsp;&nbsp;&nbsp;We planned to exame the expression of gene cheZ and trkD on protein level. Within the inducement of 0.1mg/mL Isopropyl β-D-1-thiogalactopyranoside(IPTG) at 18℃ for 15~18h, we extracted the total proteins of the E.coli transformed with CheZ-pET28a or TrkD-pET32a. Western Blot results showed that the quantity of protein CheZ and TrkD significantly increased after the inducement, indicating that cheZ and trkD expressed well. Additionally, sicne a basal expression caused by promoter lac in both plasmids, the control groups showed a small quantity of proteins. </p></div><br />
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<li> <a href="https://static.igem.org/mediawiki/igem.org/9/92/Western_blot_L.png" title=""><img src="https://static.igem.org/mediawiki/igem.org/a/ae/Westerblot_s.png" width="150" height="137" alt="Flower" /></a> </li><br />
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<h2>Cesium absorption test </h2><br />
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<div class="page_content_textBar_enterTEXT_enter"><br />
<p>&nbsp;&nbsp;&nbsp;&nbsp;We use TrkD-pET32a to test the function of trkD. The test could be accomplished through the detection of the amount of Cs+ absorption in the experiment group and control group. </p><br />
<p>&nbsp;&nbsp;&nbsp;&nbsp;The analysis of Cs+ absorption through trkD indicates that under the inducement of IPTG (0.5mM) for about 4h, the trkD constructed in plasmid can express normally, thus the experiment group has a higher amount of absorption than that of the control group. While the reason why the control has also absorbed Cs+ is that gene trkD also exists in E.coli's genome, so the control group can also express a certain amount of trkD to accomplish the absorption of Cs+. As a result, the absorption of the experiment group is 47.8% higher than that of the control group.</p><br />
<p>&nbsp;&nbsp;&nbsp;&nbsp;In terms of the result, we have proved that the trkD constructed in the plasmid can function normally. </p><br />
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<h2>Characterizing Promoters</h2><br />
<br /><br />
<div class="page_content_textBar_enterTEXT_enter"> <P>&nbsp;&nbsp;&nbsp;&nbsp;Because we want to characterize recA and recN promoters, we turned to Microplate Reader, a machine that can measure the overall fluorescent intensity. We set the exciting wave at 488nm and the emission wave at 520nm, which allow us the measure the fluorescence of recA-GFP-pET28a-BL21 and recA-GFP-pET28a-BL21. After induce with NAL and regulate the OD value of each inocula to a same value, we measured the fluorescence intensity of recA-GFP-pET28a-BL21 and recA-GFP-pET28a-BL21.</P><br />
<p>&nbsp;&nbsp;&nbsp;&nbsp;About the two crests of the curve of recA-GFP-pET28a-BL21, we guess that the rec promoter inside the genome of the BL21 itself, beside the one caused by rec promoter in the plasmid, may be the reason of another crest. At first, the recA promoter is triggered by the increasing NAL concentration, which leads to the first crest. As the NAL concentration increases, the DNA of the plasmid was damaged but the rec promoter in the genome has not been triggered yet since the concentration of the NAL has not reached its threshold, which results in the trough between the two crests. Finally, the rec promoter in the genome is triggered by the relatively high concentration of NAL and produces the protein of rec family, which may help repair the DNA of the plasmid, which causes the second crest of the curve.</p><br />
<p>&nbsp;&nbsp;&nbsp;&nbsp;The curve of recN-GFP-pET28a-BL21 seems to support our hypothesis. The curve of recN-GFP-pET28a-BL21 has a platform instead of a trough. Then a crest after that. We guess that the threshold of recN promoter is higher than recA promoter is the reason. The crest caused by the recN promoter in the plasmid overlappes with the trough, which creates a platform as we see.</p><br />
<p>&nbsp;&nbsp;&nbsp;&nbsp;As the why the the rec promoter of the plasmid will be damaged earlier than that in the genome, we guess the different position of the two promoters is the reason.</p><br />
<p>&nbsp;&nbsp;&nbsp;&nbsp;However, the difference between all the data is not convincing enough, which may lead to another explanation: all the tendency of the data is caused by system error, and yet, which we haven't found out. </p><br />
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INSERT ANIMINATION <br />
INSERT ANIMINATION <br />
INSERT ANIMINATION <br />
INSERT ANIMINATION <br />
INSERT ANIMINATION <br />
INSERT ANIMINATION INSERT ANIMINATION <br />
INSERT ANIMINATION <br />
INSERT ANIMINATION<br />
INSERT ANIMINATION <br />
INSERT ANIMINATION <br />
INSERT ANIMINATION <br />
INSERT ANIMINATION <br />
INSERT ANIMINATION <br />
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INSERT ANIMINATION INSERT ANIMINATION <br />
<br />
</div><br />
<div class="page_content_relatedBar_project_mc"><br />
<h2>Pages we think helpful:</h2><br />
<h1><a href="https://2011.igem.org/Team:SYSU-China/page_project_modules_construction">see how we construct our modules</a></h1><br />
<h1><a href="https://2011.igem.org/Team:SYSU-China/page_project_modules_verification">see how we verify each module</a></h1><br />
<h1><a href="https://2011.igem.org/Team:SYSU-China/main_page_story">or see our whole idea in Story page</a></h1><br />
<p>&nbsp;</p><br />
</div><br />
</div> <br />
<!---------------------------------------------!--><br />
<div class="main_page_clearer_light"></div><br />
<!---------------------------------------------!--> <br />
<br />
</div><!--here ends the page_content_WholeBox!--><br />
<div class="main_page_clearer_light"></div><br />
</div><!--here ends the mainpage_project_WholeContainer!--><br />
<br />
<!--here begins the contact_bottombar!--><br />
<div class="contact_bottombar"><br />
<p>From the 2011 iGEM team SYSU-China (2011)</p><br />
<p>Sun Yat-Sen University, Guangzhou, China</p><br />
<p>Address: 135# Xingang Rd.(W.), Haizhu Guangzhou, P.R.China</p><br />
<p><a href="http://eng.sysu.edu.cn/">visit the Sun Yat-sen university website</a></p><br />
<p>Thanks Apycom jQuery Menus and <a href="http://apycom.com/">visit their website</a><br />
</div><br />
<!--here ends the contact_bottombar!--><br />
<br />
</div><!--here ends the backgroud_shadow_bar!--><br />
</body><br />
</html></div>Cambihttp://2011.igem.org/Team:SYSU-China/page_aboutus_special_thanksTeam:SYSU-China/page aboutus special thanks2011-10-06T02:55:16Z<p>Cambi: </p>
<hr />
<div>{{:Team:SYSU-China/header/temp}}<br />
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<html xmlns="http://www.w3.org/1999/xhtml"><br />
<head><br />
<meta http-equiv="Content-Type" content="text/html; charset=utf-8" /><br />
<title>Special Thanks-Sun Yat-sen Univ.</title><br />
<link type="text/css" href="menu.css" rel="stylesheet" /><br />
<script type="text/javascript" src="jquery.js"></script><br />
<script type="text/javascript" src="menu.js"></script><br />
<script type="text/javascript" src="scrollTo.js"></script><br />
<link href="style.css" rel="stylesheet" type="text/css" /><br />
<script type="text/javascript" language="javascript"><br />
$(function(){<br />
// $("#page_content_baBar_details").scrollTo(600,2横向)<br />
<br />
//<br />
// <br />
$("#page_content_baBar_details").scrollTo(700)<br />
});</script><br />
</head><br />
<br />
<body><br />
<div id="background_shadow"><br />
<div class="page_aboutus_WholeContainer"> <br />
<br />
<!--------here begins the manu bar section--!--><br />
<div class="title_container"><br />
<div id="menu"><br />
<ul class="menu"><br />
<li><a href="index.thml"><span></span></a></li><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/main_page_news"><span>NEWS</span></a></li><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/main_page_story"><span>STORY</span></a></li> <br />
<li><a href="https://2011.igem.org/Team:SYSU-China/main_page_project" class="parent"><span>PROJECT</span></a><br />
<div><br />
<ul><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/page_project_modules_construction"><span>Modules Construction</span></a><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/page_project_modules_verification"><span>Modules Verification</span></a></li><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/page_project_functional_construction"><span>Functional Verification</span></a></li><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/page_project_notes"><span>Notes</span></a></li><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/page_project_datapage"><span>Data Page</span></a></li><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/page_project_safety"><span>Safety</span></a></li><br />
</ul><br />
</div><br />
</li><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/main_page_human_practice" class="parent"><span>HUMAN PRACTICE</span></a><br />
<div><br />
<ul><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/page_human_practice_app"><span>App</span></a><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/page_human_practice_labcraft"><span>LabCraft Board Game</span></a></li><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/page_human_practice_workshop"><span>Workshop</span></a></li><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/page_human_practice_survey"><span>Survey</span></a></li><br />
</ul><br />
</div><br />
</li><br />
<br />
<li><a href="https://2011.igem.org/Team:SYSU-China/main_page_aboutus" class="parent"><span>ABOUT US</span></a><br />
<div><br />
<ul><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/page_aboutus_team_members"><span>Team Members</span></a><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/page_aboutus_special_thanks"><span>Spcial Thanks</span></a></li><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/page_aboutus_LT"><span>Logo and T-shirt</span></a></li><br />
</ul><br />
</div><br />
</li><br />
<br />
</ul><br />
</div><!--here ends the manu!--><br />
</div><!--here ends the title_container!--><br />
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<!--------here ends the title_section------!--><br />
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<div class="page_clear_and_introBar"><br />
<img src="https://static.igem.org/mediawiki/igem.org/8/86/SYSU_Page_aboutus_special_thanks_introBar.png<br />
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<!------here begins the main content section--!--><br />
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<!---------------------------------------------!--><br />
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<div class="page_content_bgBar"><img src="https://static.igem.org/mediawiki/igem.org/4/4d/SYSU_Page_aboutus_special_thanks_bg.jpg<br />
" width="632" height="600" /><br />
<div id="page_content_baBar_details"><a href="#page_content_textBar_enterTEXT">See Details</a></div><br />
</div><br />
<div class="page_content_aboutus_textBar"><br />
<div id="page_content_textBar_enterTEXT"><br />
<br />
<ul><br />
<li><img name="" src="https://static.igem.org/mediawiki/igem.org/6/64/SYSU_Page_aboutus_photoBG.png" width="114" height="152" alt="" /><br />
<h1>Jian REN</h1><br />
<p>Sofeware Adviser</p><br />
</li><br />
<li><img name="" src="https://static.igem.org/mediawiki/igem.org/6/64/SYSU_Page_aboutus_photoBG.png" width="114" height="152" alt="" /><br />
<h1>XiongLei HE</h1><br />
<p>Adviser</p><br />
</li><br />
<li><img name="" src="https://static.igem.org/mediawiki/igem.org/6/64/SYSU_Page_aboutus_photoBG.png" width="114" height="152" alt="" /><br />
<h1>YongJun LU</h1><br />
<p>Adviser</p><br />
</li><br />
</ul><br />
<br />
<ul> <br />
<li><img name="" src="https://static.igem.org/mediawiki/igem.org/6/64/SYSU_Page_aboutus_photoBG.png" width="114" height="152" alt="" /><br />
<h1>Chao ZHANG</h1><br />
<p>Ex-member</p><br />
</li><br />
<li><img name="" src="https://static.igem.org/mediawiki/igem.org/6/64/SYSU_Page_aboutus_photoBG.png" width="114" height="152" alt="" /><br />
<h1>JiaYan ZHOU</h1><br />
<p>Animation Narrator</p><br />
</li><br />
<li><img name="" src="https://static.igem.org/mediawiki/igem.org/6/64/SYSU_Page_aboutus_photoBG.png" width="114" height="152" alt="" /><br />
<h1>YanQiu YANG</h1><br />
<p>Animation Producer</p><br />
</li><br />
<li><img name="" src="https://static.igem.org/mediawiki/igem.org/6/64/SYSU_Page_aboutus_photoBG.png" width="114" height="152" alt="" /><br />
<h1>Yi YANG</h1><br />
<p>A really cute girl& Ex-member</p><br />
</li><br />
<li><img name="" src="https://static.igem.org/mediawiki/igem.org/6/64/SYSU_Page_aboutus_photoBG.png" width="114" height="152" alt="" /><br />
<h1>ZeDa ZHANG</h1><br />
<p>Ex-member</p><br />
<p>Best friend of Wiki Coder ^ ^</p><br />
<p>God bless you</p><br />
</li><br />
</ul><br />
<ul><br />
<li id="fuking_tired"><br />
<h1>BinBin ZHENG</h1><br />
<p>&nbsp;&nbsp;&nbsp;&nbsp;Young enthusiastic biosynthetic student</p></li><br />
<li id="fuking_tired"><br />
<h1>PengHui YUAN</h1><br />
<p>&nbsp;&nbsp;&nbsp;&nbsp;Young enthusiastic biosynthetic student</p></li><br />
<li id="fuking_tired"><br />
<h1>Xia'Nan FU</h1><br />
<p>&nbsp;&nbsp;&nbsp;&nbsp;Young enthusiastic biosynthetic student</p></li><br />
<li id="fuking_tired"><br />
<h1>YunHao SUN</h1><br />
<p>&nbsp;&nbsp;&nbsp;&nbsp;Thank you Bro. Sui, for the hapiness you have brought to us</p></li><br />
<li id="fuking_tired"><br /><br /><br /></li> <br />
<li id="fuking_tired"><p>etc. We thank you all for helping us during the experiments and the making of the whole project.</p></li><br />
<br />
</ul> <br />
</div> <br />
</div><br />
</div><br />
<!---------------------------------------------!--><br />
<div class="page_content_RsideBar"><br />
<div class="page_content_relatedBar_aboutus"><br />
<h2>Pages we think helpful:</h2><br />
<h1><a href="https://2011.igem.org/Team:SYSU-China/page_aboutus_team_members">our team member page</a></h1><br />
<h1><a href="https://2011.igem.org/Team:SYSU-China/main_page_human_practice">our human practice page</a></h1><br />
<p>&nbsp;</p><br />
</div><br />
</div> <br />
<!---------------------------------------------!--><br />
<div class="main_page_clearer_light"></div><br />
<!---------------------------------------------!--> <br />
<br />
</div><!--here ends the page_content_WholeBox!--><br />
<div class="main_page_clearer_light"></div><br />
</div><!--here ends the mainpage_project_WholeContainer!--><br />
<br />
<!--here begins the contact_bottombar!--><br />
<div class="contact_bottombar"><br />
<p>From the 2011 iGEM team SYSU-China (2011)</p><br />
<p>Sun Yat-Sen University, Guangzhou, China</p><br />
<p>Address: 135# Xingang Rd.(W.), Haizhu Guangzhou, P.R.China</p><br />
<p><a href="http://eng.sysu.edu.cn/">visit the Sun Yat-sen university website</a></p><br />
<p>Thanks Apycom jQuery Menus and <a href="http://apycom.com/">visit their website</a><br />
</div><br />
<!--here ends the contact_bottombar!--><br />
<br />
</div><!--here ends the backgroud_shadow_bar!--><br />
</body><br />
</html></div>Cambihttp://2011.igem.org/Team:SYSU-China/page_project_modules_verificationTeam:SYSU-China/page project modules verification2011-10-06T02:53:28Z<p>Cambi: </p>
<hr />
<div>{{:Team:SYSU-China/header/temp}}<br />
{{:Team:SYSU-China/header/jscss}}<br />
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<div id="background_shadow"><br />
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<br />
<!--------here begins the manu bar section--!--><br />
<div class="title_container"><br />
<div id="menu"><br />
<ul class="menu"><br />
<li><a href="index.thml"><span></span></a></li><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/main_page_news"><span>NEWS</span></a></li><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/main_page_story"><span>STORY</span></a></li> <br />
<li><a href="https://2011.igem.org/Team:SYSU-China/main_page_project" class="parent"><span>PROJECT</span></a><br />
<div><br />
<ul><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/page_project_modules_construction"><span>Modules Construction</span></a><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/page_project_modules_verification"><span>Modules Verification</span></a></li><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/page_project_functional_construction"><span>Functional Verification</span></a></li><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/page_project_notes"><span>Notes</span></a></li><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/page_project_datapage"><span>Date Page</span></a></li><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/page_project_safety"><span>Safety</span></a></li><br />
</ul><br />
</div><br />
</li><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/main_page_human_practice" class="parent"><span>HUMAN PRACTICE</span></a><br />
<div><br />
<ul><br />
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<h2>Fluorescence </h2><br />
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<p>&nbsp;&nbsp;&nbsp;&nbsp;We decided to examine the expression of the two promoters- PrecA and PrecN, and two genes-cheZ and trkD, via the fluorescent intensity of EGFP whose sequence had been ligated downstream after them. </p><br />
<p>&nbsp;&nbsp;&nbsp;&nbsp;We have constructed a plasmid (pUC18) with EGFP, which is ligated to the plasmid in the PstI and HindIII restriction enzyme cutting sites. Then we link PrecA,PrecN,cheZ,trkD gene to EGFP sequence respectively to construct four plasmids, recA-GFP-pUC18,recN-GFP-pUC18,cheZ-GFP-pUC18 and trkD-GFP-pUC18. We used ultraviolet or nalidixic acid to damage E.coli's DNA to trigger the expression of PrecA and PrecN on in order to examine the expression of EGFP. However, there is a lac promoter upstream before the multi-cloning site of pUC18, which allows us to use IPTG induction to examine the expression of cheZ and trkD by watching the GFP expression. In order to ensure the expression of two genes and GFP, we deleted the terminator of the two genes, cheZ and trkD.</p><br />
<p>&nbsp;&nbsp;&nbsp;&nbsp;Using the laser scanning confocal microscope (LSCM), we can see obvious fluorescence of cheZ-GFP-pUC18 and trkD-GFP-pUC18(Figure 1 and Figure 2). The fluorescence of trkD-GFP-pUC18, is located mostly on the membrane of E.coli. The lac promoter, however, will express without induction of IPTG, resulting in the expression of GFP of control group, which is cheZ-GFP-pUC18 and trkD-GFP-pUC18 that are not induced by IPTG. Consequently, we utilized the Flow Cytometer(FCM) to exam the intensity of GFP in control group and experiment group. (We are still working on that)</p><br />
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<li> <a href="https://static.igem.org/mediawiki/2011/3/3b/SYSU_1_chez-GFP-pUC18-control.jpg" title=""><img src="https://static.igem.org/mediawiki/igem.org/8/87/1_chez-GFP-pUC18-control_S.jpg" width="150" height="126" alt="Flower" /></a> </li><br />
<li> <a href="https://static.igem.org/mediawiki/2011/8/83/SYSU_2_chez-GFP-pUC18-experiment.jpg" title=""><img src="https://static.igem.org/mediawiki/igem.org/4/43/2_chez-GFP-pUC18-experiment_S.jpg" width="150" height="126" alt="Tree" /></a> </li><br />
<li> <a href="https://static.igem.org/mediawiki/2011/e/e1/SYSU_3_trkD-GFP-pUC18-control.jpg" title=""><img src="https://static.igem.org/mediawiki/igem.org/d/d1/3_trkD-GFP-pUC18-control_S.jpg" width="150" height="126" alt="" /></a> </li><br />
<li> <a href="https://static.igem.org/mediawiki/2011/d/d8/SYSU_4_trkd-GFP-pUC18-experiment.jpg" title=""><img src="https://static.igem.org/mediawiki/igem.org/8/81/4_trkd-GFP-pUC18-experiment_S.jpg" width="150" height="126" alt="" /></a> </li><br />
<li> <a href="https://static.igem.org/mediawiki/2011/a/a8/SYSU_5_trkD-GFP-pUC18-control1.jpg" title=""><img src="https://static.igem.org/mediawiki/igem.org/3/36/5_trkD-GFP-pUC18-control1_S.jpg" width="150" height="126" alt="" /></a> </li><br />
<li> <a href="https://static.igem.org/mediawiki/2011/0/0f/SYSU_6_trkd-GFP-pUC18-experiment1.jpg" title=""><img src="https://static.igem.org/mediawiki/igem.org/8/84/6_trkd-GFP-pUC18-experiment1_S.jpg" width="150" height="126" alt="" /></a> </li><br />
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<h2>Western Blot test</h2><br />
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<div class="page_content_textBar_enterTEXT_enter"><p>&nbsp;&nbsp;&nbsp;&nbsp;We planned to exame the expression of gene cheZ and trkD on protein level. Within the inducement of 0.1mg/mL Isopropyl β-D-1-thiogalactopyranoside(IPTG) at 18℃ for 15~18h, we extracted the total proteins of the E.coli transformed with CheZ-pET28a or TrkD-pET32a. Western Blot results showed that the quantity of protein CheZ and TrkD significantly increased after the inducement, indicating that cheZ and trkD expressed well. Additionally, sicne a basal expression caused by promoter lac in both plasmids, the control groups showed a small quantity of proteins. </p></div><br />
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<h2>Cesium absorption test </h2><br />
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<p>&nbsp;&nbsp;&nbsp;&nbsp;We use TrkD-pET32a to test the function of trkD. The test could be accomplished through the detection of the amount of Cs+ absorption in the experiment group and control group. </p><br />
<p>&nbsp;&nbsp;&nbsp;&nbsp;The analysis of Cs+ absorption through trkD indicates that under the inducement of IPTG (0.5mM) for about 4h, the trkD constructed in plasmid can express normally, thus the experiment group has a higher amount of absorption than that of the control group. While the reason why the control has also absorbed Cs+ is that gene trkD also exists in E.coli's genome, so the control group can also express a certain amount of trkD to accomplish the absorption of Cs+. As a result, the absorption of the experiment group is 47.8% higher than that of the control group.</p><br />
<p>&nbsp;&nbsp;&nbsp;&nbsp;In terms of the result, we have proved that the trkD constructed in the plasmid can function normally. </p><br />
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<h2>Characterizing Promoters</h2><br />
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<div class="page_content_textBar_enterTEXT_enter"> <P>&nbsp;&nbsp;&nbsp;&nbsp;Because we want to characterize recA and recN promoters, we turned to Microplate Reader, a machine that can measure the overall fluorescent intensity. We set the exciting wave at 488nm and the emission wave at 520nm, which allow us the measure the fluorescence of recA-GFP-pET28a-BL21 and recA-GFP-pET28a-BL21. After induce with NAL and regulate the OD value of each inocula to a same value, we measured the fluorescence intensity of recA-GFP-pET28a-BL21 and recA-GFP-pET28a-BL21.</P><br />
<p>&nbsp;&nbsp;&nbsp;&nbsp;About the two crests of the curve of recA-GFP-pET28a-BL21, we guess that the rec promoter inside the genome of the BL21 itself, beside the one caused by rec promoter in the plasmid, may be the reason of another crest. At first, the recA promoter is triggered by the increasing NAL concentration, which leads to the first crest. As the NAL concentration increases, the DNA of the plasmid was damaged but the rec promoter in the genome has not been triggered yet since the concentration of the NAL has not reached its threshold, which results in the trough between the two crests. Finally, the rec promoter in the genome is triggered by the relatively high concentration of NAL and produces the protein of rec family, which may help repair the DNA of the plasmid, which causes the second crest of the curve.</p><br />
<p>&nbsp;&nbsp;&nbsp;&nbsp;The curve of recN-GFP-pET28a-BL21 seems to support our hypothesis. The curve of recN-GFP-pET28a-BL21 has a platform instead of a trough. Then a crest after that. We guess that the threshold of recN promoter is higher than recA promoter is the reason. The crest caused by the recN promoter in the plasmid overlappes with the trough, which creates a platform as we see.</p><br />
<p>&nbsp;&nbsp;&nbsp;&nbsp;As the why the the rec promoter of the plasmid will be damaged earlier than that in the genome, we guess the different position of the two promoters is the reason.</p><br />
<p>&nbsp;&nbsp;&nbsp;&nbsp;However, the difference between all the data is not convincing enough, which may lead to another explanation: all the tendency of the data is caused by system error, and yet, which we haven't found out. </p><br />
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<h2>Pages we think helpful:</h2><br />
<h1><a href="https://2011.igem.org/Team:SYSU-China/page_project_modules_construction">see how we construct our modules</a></h1><br />
<h1><a href="https://2011.igem.org/Team:SYSU-China/page_project_modules_verification">see how we verify each module</a></h1><br />
<h1><a href="https://2011.igem.org/Team:SYSU-China/main_page_story">or see our whole idea in Story page</a></h1><br />
<p>&nbsp;</p><br />
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<p>From the 2011 iGEM team SYSU-China (2011)</p><br />
<p>Sun Yat-Sen University, Guangzhou, China</p><br />
<p>Address: 135# Xingang Rd.(W.), Haizhu Guangzhou, P.R.China</p><br />
<p><a href="http://eng.sysu.edu.cn/">visit the Sun Yat-sen university website</a></p><br />
<p>Thanks Apycom jQuery Menus and <a href="http://apycom.com/">visit their website</a><br />
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</html></div>Cambihttp://2011.igem.org/Team:SYSU-China/page_project_modules_verificationTeam:SYSU-China/page project modules verification2011-10-06T02:42:13Z<p>Cambi: </p>
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<li><a href="https://2011.igem.org/Team:SYSU-China/main_page_news"><span>NEWS</span></a></li><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/main_page_story"><span>STORY</span></a></li> <br />
<li><a href="https://2011.igem.org/Team:SYSU-China/main_page_project" class="parent"><span>PROJECT</span></a><br />
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<li><a href="https://2011.igem.org/Team:SYSU-China/page_project_modules_construction"><span>Modules Construction</span></a><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/page_project_modules_verification"><span>Modules Verification</span></a></li><br />
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<li><a href="https://2011.igem.org/Team:SYSU-China/page_project_notes"><span>Notes</span></a></li><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/page_project_datapage"><span>Date Page</span></a></li><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/page_project_safety"><span>Safety</span></a></li><br />
</ul><br />
</div><br />
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<li><a href="https://2011.igem.org/Team:SYSU-China/main_page_human_practice" class="parent"><span>HUMAN PRACTICE</span></a><br />
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<ul><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/page_human_practice_app"><span>App</span></a><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/page_human_practice_labcraft"><span>LabCraft Board Game</span></a></li><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/page_human_practice_workshop"><span>Workshop</span></a></li><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/page_human_practice_survey"><span>Survey</span></a></li><br />
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</li><br />
<br />
<li><a href="https://2011.igem.org/Team:SYSU-China/main_page_aboutus" class="parent"><span>ABOUT US</span></a><br />
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<li><a href="https://2011.igem.org/Team:SYSU-China/page_aboutus_team_members"><span>Team Members</span></a><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/page_aboutus_special_thanks"><span>Spcial Thanks</span></a></li><br />
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<div id="page_content_baBar_details"><a href="#TabbedPanels2">See Details</a></div><br />
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<div class="page_content_textBar"><br />
<div id="TabbedPanels2"><br />
<h2>Fluorescence </h2><br />
<div class="page_content_textBar_enterTEXT_enter"><br />
<br /><br />
<p>&nbsp;&nbsp;&nbsp;&nbsp;We decided to examine the expression of the two promoters- PrecA and PrecN, and two genes-cheZ and trkD, via the fluorescent intensity of EGFP whose sequence had been ligated downstream after them. </p><br />
<p>&nbsp;&nbsp;&nbsp;&nbsp;We have constructed a plasmid (pUC18) with EGFP, which is ligated to the plasmid in the PstI and HindIII restriction enzyme cutting sites. Then we link PrecA,PrecN,cheZ,trkD gene to EGFP sequence respectively to construct four plasmids, recA-GFP-pUC18,recN-GFP-pUC18,cheZ-GFP-pUC18 and trkD-GFP-pUC18. We used ultraviolet or nalidixic acid to damage E.coli's DNA to trigger the expression of PrecA and PrecN on in order to examine the expression of EGFP. However, there is a lac promoter upstream before the multi-cloning site of pUC18, which allows us to use IPTG induction to examine the expression of cheZ and trkD by watching the GFP expression. In order to ensure the expression of two genes and GFP, we deleted the terminator of the two genes, cheZ and trkD.</p><br />
<p>&nbsp;&nbsp;&nbsp;&nbsp;Using the laser scanning confocal microscope (LSCM), we can see obvious fluorescence of cheZ-GFP-pUC18 and trkD-GFP-pUC18(Figure 1 and Figure 2). The fluorescence of trkD-GFP-pUC18, is located mostly on the membrane of E.coli. The lac promoter, however, will express without induction of IPTG, resulting in the expression of GFP of control group, which is cheZ-GFP-pUC18 and trkD-GFP-pUC18 that are not induced by IPTG. Consequently, we utilized the Flow Cytometer(FCM) to exam the intensity of GFP in control group and experiment group. (We are still working on that)</p><br />
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<ul><br />
<li> <a href="https://static.igem.org/mediawiki/2011/3/3b/SYSU_1_chez-GFP-pUC18-control.jpg" title=""><img src="https://static.igem.org/mediawiki/igem.org/8/87/1_chez-GFP-pUC18-control_S.jpg" width="150" height="126" alt="Flower" /></a> <p>chez-GFP-pUC18-control</p></li><br />
<li> <a href="https://static.igem.org/mediawiki/2011/8/83/SYSU_2_chez-GFP-pUC18-experiment.jpg" title=""><img src="https://static.igem.org/mediawiki/igem.org/4/43/2_chez-GFP-pUC18-experiment_S.jpg" width="150" height="126" alt="Tree" /></a><p>chez-GFP-pUC18-experiment</p></li><br />
<li> <a href="https://static.igem.org/mediawiki/2011/e/e1/SYSU_3_trkD-GFP-pUC18-control.jpg" title=""><img src="https://static.igem.org/mediawiki/igem.org/d/d1/3_trkD-GFP-pUC18-control_S.jpg" width="150" height="126" alt="" /></a><p>trkD-GFP-pUC18-control</p> </li><br />
<li> <a href="https://static.igem.org/mediawiki/2011/d/d8/SYSU_4_trkd-GFP-pUC18-experiment.jpg" title=""><img src="https://static.igem.org/mediawiki/igem.org/8/81/4_trkd-GFP-pUC18-experiment_S.jpg" width="150" height="126" alt="" /></a><p>trkd-GFP-pUC18-experiment</p></li><br />
<li> <a href="https://static.igem.org/mediawiki/2011/a/a8/SYSU_5_trkD-GFP-pUC18-control1.jpg" title=""><img src="https://static.igem.org/mediawiki/igem.org/3/36/5_trkD-GFP-pUC18-control1_S.jpg" width="150" height="126" alt="" /></a><p>trkD-GFP-pUC18-control</li><br />
<li> <a href="https://static.igem.org/mediawiki/2011/0/0f/SYSU_6_trkd-GFP-pUC18-experiment1.jpg" title=""><img src="https://static.igem.org/mediawiki/igem.org/8/84/6_trkd-GFP-pUC18-experiment1_S.jpg" width="150" height="126" alt="" /></a><p>trkd-GFP-pUC18-experiment</p></li><br />
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<h2>Western Blot test</h2><br />
<br /><br />
<div class="page_content_textBar_enterTEXT_enter"><p>&nbsp;&nbsp;&nbsp;&nbsp;We planned to exame the expression of gene cheZ and trkD on protein level. Within the inducement of 0.1mg/mL Isopropyl β-D-1-thiogalactopyranoside(IPTG) at 18℃ for 15~18h, we extracted the total proteins of the E.coli transformed with CheZ-pET28a or TrkD-pET32a. Western Blot results showed that the quantity of protein CheZ and TrkD significantly increased after the inducement, indicating that cheZ and trkD expressed well. Additionally, sicne a basal expression caused by promoter lac in both plasmids, the control groups showed a small quantity of proteins. </p></div><br />
<div class="page_content_text_Bar_enterTEXT_RpicBar"><br />
<div id="gallery" class="lbGallery"><br />
<ul><br />
<li> <a href="https://static.igem.org/mediawiki/igem.org/9/92/Western_blot_L.png" title=""><img src="https://static.igem.org/mediawiki/igem.org/a/ae/Westerblot_s.png" width="150" height="137" alt="Flower" /></a> </li><br />
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<br />
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<h2>Cesium absorption test </h2><br />
<br /><br />
<div class="page_content_textBar_enterTEXT_enter"><br />
<p>&nbsp;&nbsp;&nbsp;&nbsp;We use TrkD-pET32a to test the function of trkD. The test could be accomplished through the detection of the amount of Cs+ absorption in the experiment group and control group. </p><br />
<p>&nbsp;&nbsp;&nbsp;&nbsp;The analysis of Cs+ absorption through trkD indicates that under the inducement of IPTG (0.5mM) for about 4h, the trkD constructed in plasmid can express normally, thus the experiment group has a higher amount of absorption than that of the control group. While the reason why the control has also absorbed Cs+ is that gene trkD also exists in E.coli's genome, so the control group can also express a certain amount of trkD to accomplish the absorption of Cs+. As a result, the absorption of the experiment group is 47.8% higher than that of the control group.</p><br />
<p>&nbsp;&nbsp;&nbsp;&nbsp;In terms of the result, we have proved that the trkD constructed in the plasmid can function normally. </p><br />
</div><br />
<h2>Characterizing Promoters</h2><br />
<br /><br />
<div class="page_content_textBar_enterTEXT_enter"> <P>&nbsp;&nbsp;&nbsp;&nbsp;Because we want to characterize recA and recN promoters, we turned to Microplate Reader, a machine that can measure the overall fluorescent intensity. We set the exciting wave at 488nm and the emission wave at 520nm, which allow us the measure the fluorescence of recA-GFP-pET28a-BL21 and recA-GFP-pET28a-BL21. After induce with NAL and regulate the OD value of each inocula to a same value, we measured the fluorescence intensity of recA-GFP-pET28a-BL21 and recA-GFP-pET28a-BL21.</P><br />
<p>&nbsp;&nbsp;&nbsp;&nbsp;About the two crests of the curve of recA-GFP-pET28a-BL21, we guess that the rec promoter inside the genome of the BL21 itself, beside the one caused by rec promoter in the plasmid, may be the reason of another crest. At first, the recA promoter is triggered by the increasing NAL concentration, which leads to the first crest. As the NAL concentration increases, the DNA of the plasmid was damaged but the rec promoter in the genome has not been triggered yet since the concentration of the NAL has not reached its threshold, which results in the trough between the two crests. Finally, the rec promoter in the genome is triggered by the relatively high concentration of NAL and produces the protein of rec family, which may help repair the DNA of the plasmid, which causes the second crest of the curve.</p><br />
<p>&nbsp;&nbsp;&nbsp;&nbsp;The curve of recN-GFP-pET28a-BL21 seems to support our hypothesis. The curve of recN-GFP-pET28a-BL21 has a platform instead of a trough. Then a crest after that. We guess that the threshold of recN promoter is higher than recA promoter is the reason. The crest caused by the recN promoter in the plasmid overlappes with the trough, which creates a platform as we see.</p><br />
<p>&nbsp;&nbsp;&nbsp;&nbsp;As the why the the rec promoter of the plasmid will be damaged earlier than that in the genome, we guess the different position of the two promoters is the reason.</p><br />
<p>&nbsp;&nbsp;&nbsp;&nbsp;However, the difference between all the data is not convincing enough, which may lead to another explanation: all the tendency of the data is caused by system error, and yet, which we haven't found out. </p><br />
</div><br />
<div class="page_content_text_Bar_enterTEXT_RpicBar"><br />
<div id="gallery" class="lbGallery"><br />
<ul><br />
<li> <a href="https://static.igem.org/mediawiki/igem.org/f/f3/1.jpg" title=""><img src="https://static.igem.org/mediawiki/igem.org/8/82/Mb1S.png" width="150" height="88" alt="Flower" /></a> <br />
<p>Figure1.&nbsp;&nbsp;&nbsp;&nbsp;Fluorescence intensity of recA-GFP-pET28a-BL21 in the first and second experiments</p><br />
</li><br />
<li> <a href="https://static.igem.org/mediawiki/igem.org/0/0b/Mb2.png" title=""><img src="https://static.igem.org/mediawiki/igem.org/4/41/Mb2S.png" width="150" height="92" alt="Flower" /></a> <p>Figure2.&nbsp;&nbsp;&nbsp;&nbsp;Fluorescence intensity of recA-GFP-pET28a-BL21 in the third and forth experiments</p><br />
</li><br />
<li> <a href="https://static.igem.org/mediawiki/igem.org/c/c5/Mb3.png" title=""><img src="https://static.igem.org/mediawiki/igem.org/d/df/Mb3S.png" width="150" height="99" alt="Flower" /></a> <p>Figure3.&nbsp;&nbsp;&nbsp;&nbsp;Fluorescence intensity of recN-GFP-pET28a-BL21 in all the five experiments</p> </li><br />
</ul><br />
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<h1>Please watch our animination</h1><br />
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<h2>Pages we think helpful:</h2><br />
<h1><a href="https://2011.igem.org/Team:SYSU-China/page_project_modules_construction">see how we construct our modules</a></h1><br />
<h1><a href="https://2011.igem.org/Team:SYSU-China/page_project_modules_verification">see how we verify each module</a></h1><br />
<h1><a href="https://2011.igem.org/Team:SYSU-China/main_page_story">or see our whole idea in Story page</a></h1><br />
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<p>From the 2011 iGEM team SYSU-China (2011)</p><br />
<p>Sun Yat-Sen University, Guangzhou, China</p><br />
<p>Address: 135# Xingang Rd.(W.), Haizhu Guangzhou, P.R.China</p><br />
<p><a href="http://eng.sysu.edu.cn/">visit the Sun Yat-sen university website</a></p><br />
<p>Thanks Apycom jQuery Menus and <a href="http://apycom.com/">visit their website</a><br />
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}<br />
<br />
ul#output li a {<br />
position: absolute;<br />
bottom: 10px;<br />
right: 10px;<br />
padding: 8px 12px;<br />
text-decoration: none;<br />
font-size: 11px;<br />
color: #FFF;<br />
background: #000;<br />
-moz-border-radius: 5px;<br />
}<br />
<br />
ul#output li a:hover {<br />
background: #D33431;<br />
}<br />
<br />
<br />
#logo_bar {<br />
position:relative;<br />
width:93px;<br />
height:125px;<br />
z-index:5;<br />
left: 20px;<br />
top: -75px;<br />
background-image: url(https://static.igem.org/mediawiki/igem.org/b/bd/SYSU_Logo_green.png);<br />
}<br />
<br />
.contact_bottombar {<br />
margin: 0px;<br />
width: 1000px;<br />
font-family: Arial, Helvetica, sans-serif;<br />
padding: 20px;<br />
font-size: 12px;<br />
}<br />
.title_container {<br />
width: 960px;<br />
margin-right: 20px;<br />
margin-left: 20px;<br />
z-index: 5;<br />
}<br />
<br />
.mainpage_project_WholeContainer {<br />
background-color: #5a7b22;<br />
background-image: url(https://static.igem.org/mediawiki/2011/6/65/SYSU_Project.png);<br />
background-position: top;<br />
width: 1000px;<br />
background-repeat: no-repeat;<br />
}<br />
.mainpage_human_practice_WholeContainer {<br />
background-color: #cda901;<br />
background-image: url(https://static.igem.org/mediawiki/igem.org/8/87/SYSU_main_pages_BG_pics_Human_practice.png<br />
);<br />
background-position: top;<br />
width: 1000px;<br />
background-repeat: no-repeat;<br />
}<br />
.mainpage_aboutus_WholeContainer {<br />
background-color: #000;<br />
background-image: url(https://static.igem.org/mediawiki/igem.org/d/d9/SYSU_main_pages_BG_pics_Aboutus.png<br />
);<br />
background-position: top;<br />
width: 1000px;<br />
background-repeat: no-repeat;<br />
}<br />
.mainpage_human_practice_WholeContainer {<br />
background-color: #cda901;<br />
background-image: url(https://static.igem.org/mediawiki/igem.org/8/87/SYSU_main_pages_BG_pics_Human_practice.png<br />
);<br />
background-position: top;<br />
width: 1000px;<br />
background-repeat: no-repeat;<br />
}<br />
.page_WholeContainer {<br />
width: 1000px;<br />
}<br />
.page_aboutus_WholeContainer {<br />
width: 1000px;<br />
background-color: #000;<br />
}<br />
<br />
<br />
#main_page_clearer {<br />
background-color: transparent;<br />
height: 535px;<br />
width: 1000px;<br />
clear: both;<br />
}<br />
<br />
.main_page_clearer_light {<br />
background-color: transparent;<br />
height: 20px;<br />
width: 100%;<br />
clear: both;<br />
}<br />
#main_page_content_WholeBox {<br />
width: 1000px;<br />
padding-top: 0px;<br />
padding-right: 20px;<br />
padding-bottom: 0px;<br />
padding-left: 20px;<br />
background-color: transparent;<br />
height: auto;<br />
}<br />
.main_page_content_box {<br />
background-color: transparent;<br />
float: left;<br />
width: 306px;<br />
margin-top: 20px;<br />
margin-right: 20px;<br />
margin-bottom: 0px;<br />
margin-left: 0px;<br />
height: auto;<br />
}<br />
.main_page_content_box_r0 {<br />
background-color: transparent;<br />
float: left;<br />
width: 306px;<br />
margin-top: 20px;<br />
margin-right: 0px;<br />
margin-bottom: 20px;<br />
margin-left: 0px;<br />
<br />
}<br />
.main_page_introduction_bar {<br />
padding: 0px;<br />
float: left;<br />
height: 34px;<br />
width: 227px;<br />
margin-top: 0px;<br />
margin-right: 4px;<br />
margin-bottom: 0px;<br />
margin-left: 0px;<br />
}<br />
.main_page_introduction_bar p {<br />
font-size: 18px;<br />
color: #000;<br />
font-family: Arial, Helvetica, sans-serif;<br />
text-align: center;<br />
vertical-align: middle;<br />
}<br />
.main_page_content_more_bar a{<br />
float: right;<br />
height: 34px;<br />
width: 75px;<br />
background-image: url(https://static.igem.org/mediawiki/igem.org/f/f2/SYSU_main_pages_BG_pics_More.png<br />
);<br />
clear: right;<br />
}<br />
.main_page_content_more_bar a:hover {<br />
background-image: url(https://static.igem.org/mediawiki/igem.org/4/40/SYSU_main_pages_BG_pics_More_light.png<br />
);<br />
float: right;<br />
height: 34px;<br />
width: 75px;<br />
clear: right;<br />
}<br />
.main_page_content_picbox {<br />
height: 136px;<br />
width: 100%;<br />
float: left;<br />
}<br />
.main_page_content_textbox {<br />
background-color: #d9d9d9;<br />
width: 100%;<br />
float: left;<br />
overflow: hidden;<br />
padding-top: 12px;<br />
padding-bottom: 12px;<br />
line-height: 1.5em;<br />
}<br />
.main_page_content_Whole_intro_bar {<br />
clear: right;<br />
float: left;<br />
width: 100%;<br />
margin-top: 0px;<br />
margin-right: 0px;<br />
margin-bottom: 5px;<br />
margin-left: 0px;<br />
}<br />
<br />
.main_page_content_textbox p {<br />
font-family: Arial, Helvetica, sans-serif;<br />
font-size: 14px;<br />
padding-right: 12px;<br />
padding-left: 12px;<br />
}<br />
.main_page_content_box_r0 .main_page_content_textbox p {<br />
font-family: Arial, Helvetica, sans-serif;<br />
font-size: 14px;<br />
}<br />
.page_clear_and_introBar {<br />
height: 65px;<br />
width: 100%;<br />
margin-top: -50px;<br />
margin-right: 0px;<br />
margin-bottom: 5px;<br />
margin-left: 20px;<br />
}<br />
#page_content_WholeBox {<br />
width: 1000px;<br />
padding-top: 0px;<br />
padding-right: 20px;<br />
padding-bottom: 0px;<br />
padding-left: 20px;<br />
background-color: transparent;<br />
height: auto;<br />
}<br />
.page_content_bgBar {<br />
height: 600px;<br />
width: 632px;<br />
margin-bottom: 20px;<br />
position: relative;<br />
}<br />
.page_content_LsideBar {<br />
float: left;<br />
height: auto;<br />
width: 632px;<br />
}<br />
.page_content_textBar {<br />
width: 632px;<br />
background-color: #f5f5f5;<br />
font-family: Arial, Helvetica, sans-serif;<br />
font-size: 14px;<br />
border: 1px solid #d9d9d9;<br />
padding: 0px;<br />
height: auto;<br />
}<br />
.page_content_aboutus_textBar {<br />
width: 632px;<br />
background-color: #292f2f;<br />
font-family: Arial, Helvetica, sans-serif;<br />
font-size: 14px;<br />
padding: 0px;<br />
float: left;<br />
}<br />
.page_content_aboutus1_textBar {<br />
width: 632px;<br />
background-color: #292f2f;<br />
font-family: Arial, Helvetica, sans-serif;<br />
font-size: 14px;<br />
padding: 0px;<br />
float: left;<br />
}<br />
<br />
#page_content_textBar_enterTEXT {<br />
padding: 12px;<br />
width: 608px;<br />
line-height: 1.75em;<br />
font-size: 14px;<br />
}<br />
.page_content_LsideBar .page_content_textBar #page_content_textBar_enterTEXT p {<br />
font-family: Arial, Helvetica, sans-serif;<br />
}<br />
/*about us 的文字在这改!!!!!!*/<br />
.page_content_LsideBar .page_content_aboutus_textBar #page_content_textBar_enterTEXT p {<br />
font-family: Arial, Helvetica, sans-serif;<br />
color: #FFF;<br />
}<br />
<br />
.page_content_RsideBar {<br />
float: left;<br />
width: 307px;<br />
margin-left: 20px;<br />
}<br />
#page_content_WholeBox .page_content_RsideBar p {<br />
font-family: Arial, Helvetica, sans-serif;<br />
}<br />
<br />
.page_content_videoBar {<br />
background-color: #484848;<br />
width: 100%;<br />
}<br />
#page_content_baBar_details a {<br />
background-color: #333;<br />
display: block;<br />
height: 25px;<br />
width: 100px;<br />
position: absolute;<br />
top: 480px;<br />
left: 525px;<br />
font-family: Arial, Helvetica, sans-serif;<br />
font-size: 16px;<br />
font-weight: bold;<br />
text-decoration: none;<br />
color: #FFF;<br />
text-align: center;<br />
padding-top: 6px;<br />
}<br />
#page_content_baBar_details a:hover {<br />
display: block;<br />
height: 25px;<br />
width: 100px;<br />
position: absolute;<br />
top: 480px;<br />
left: 525px;<br />
font-family: Arial, Helvetica, sans-serif;<br />
font-size: 16px;<br />
font-weight: bold;<br />
text-decoration: none;<br />
color: #FFF;<br />
text-align: center;<br />
padding-top: 5px;<br />
background-color: #D33431;<br />
}<br />
<br />
/*--------------various relatedBar------------*/<br />
.page_content_relatedBar_project_mc {<br />
background-color: #57a226;<br />
width: 283px;<br />
position: relative;<br />
margin-top: 20px;<br />
background-image: url(https://static.igem.org/mediawiki/igem.org/8/87/SYSU_Page_project_modules_construction_relatedBar.png);<br />
background-repeat: repeat-x;<br />
background-position: top;<br />
padding: 12px;<br />
word-wrap: break-word;<br />
word-break: break-all;<br />
font-family: Arial, Helvetica, sans-serif;<br />
}<br />
.page_content_relatedBar_project_mv {<br />
background-color: #25A025;<br />
width: 283px;<br />
position: relative;<br />
margin-top: 20px;<br />
background-image: url(https://static.igem.org/mediawiki/igem.org/3/39/SYSU_Page_project_modules_verification_relatedBar.png<br />
);<br />
background-repeat: repeat-x;<br />
background-position: top;<br />
padding: 12px;<br />
word-wrap: break-word;<br />
word-break: break-all;<br />
font-family: Arial, Helvetica, sans-serif;<br />
}<br />
.page_content_relatedBar_project_fv {<br />
background-color: #0169DF;<br />
width: 283px;<br />
position: relative;<br />
margin-top: 20px;<br />
background-image: url(https://static.igem.org/mediawiki/igem.org/6/6c/SYSU_Page_project_functional_verification_relatedBar.png<br />
);<br />
background-repeat: repeat-x;<br />
background-position: top;<br />
padding: 12px;<br />
word-wrap: break-word;<br />
word-break: break-all;<br />
font-family: Arial, Helvetica, sans-serif;<br />
}<br />
.page_content_relatedBar_project_sf {<br />
background-color: #CE8300;<br />
width: 283px;<br />
position: relative;<br />
margin-top: 20px;<br />
background-image: url(https://static.igem.org/mediawiki/igem.org/d/df/SYSU_Page_project_safety_relatedBar.png<br />
);<br />
background-repeat: repeat-x;<br />
background-position: top;<br />
padding: 12px;<br />
word-wrap: break-word;<br />
word-break: break-all;<br />
font-family: Arial, Helvetica, sans-serif;<br />
}<br />
.page_content_relatedBar_project_no {<br />
background-color: #C5AC1E;<br />
width: 283px;<br />
position: relative;<br />
margin-top: 20px;<br />
background-image: url(https://static.igem.org/mediawiki/igem.org/1/1e/SYSU_Page_project_notes_relatedBar.png<br />
);<br />
background-repeat: repeat-x;<br />
background-position: top;<br />
padding: 12px;<br />
word-wrap: break-word;<br />
word-break: break-all;<br />
font-family: Arial, Helvetica, sans-serif;<br />
}<br />
.page_content_relatedBar_project_dp {<br />
background-color: #179F55;<br />
width: 283px;<br />
position: relative;<br />
margin-top: 20px;<br />
background-image: url(https://static.igem.org/mediawiki/igem.org/5/50/SYSU_Page_project_data_page_relatedBar.png<br />
);<br />
background-repeat: repeat-x;<br />
background-position: top;<br />
padding: 12px;<br />
word-wrap: break-word;<br />
word-break: break-all;<br />
font-family: Arial, Helvetica, sans-serif;<br />
}<br />
.page_content_relatedBar_story {<br />
background-color: #0B59A4;<br />
width: 283px;<br />
position: relative;<br />
margin-top: 20px;<br />
background-image: url(https://static.igem.org/mediawiki/igem.org/4/4b/SYSU_Page_story_relatedBar.png<br />
);<br />
background-repeat: repeat-x;<br />
background-position: top;<br />
padding: 12px;<br />
word-wrap: break-word;<br />
word-break: break-all;<br />
font-family: Arial, Helvetica, sans-serif;<br />
}<br />
.page_content_relatedBar_aboutus {<br />
background-color: #484848;<br />
width: 283px;<br />
position: relative;<br />
background-image: url(https://static.igem.org/mediawiki/igem.org/6/66/SYSU_Page_aboutus_relatedBar.png<br />
);<br />
background-repeat: repeat-x;<br />
background-position: top;<br />
padding: 12px;<br />
word-wrap: break-word;<br />
word-break: break-all;<br />
font-family: Arial, Helvetica, sans-serif;<br />
color: #FFF;<br />
}<br />
.page_content_relatedBar_humanpractice_LB {<br />
background-color: #4A913B;<br />
width: 283px;<br />
position: relative;<br />
margin-top: 20px;<br />
background-image: url(https://static.igem.org/mediawiki/igem.org/c/cd/Page_human_pratice_lab_craft_relatedBar.png);<br />
background-repeat: repeat-x;<br />
background-position: top;<br />
padding: 12px;<br />
word-wrap: break-word;<br />
word-break: break-all;<br />
font-family: Arial, Helvetica, sans-serif;<br />
}<br />
.page_content_relatedBar_humanpractice_survey {<br />
background-color: #58137C;<br />
width: 283px;<br />
position: relative;<br />
margin-top: 20px;<br />
background-image: url(https://static.igem.org/mediawiki/igem.org/e/e1/Page_human_pratice_survey_relatedBar.png);<br />
background-repeat: repeat-x;<br />
background-position: top;<br />
padding: 12px;<br />
word-wrap: break-word;<br />
word-break: break-all;<br />
font-family: Arial, Helvetica, sans-serif;<br />
}<br />
.page_content_relatedBar_humanpractice_app {<br />
background-color: #022AAB;<br />
width: 283px;<br />
position: relative;<br />
margin-top: 20px;<br />
background-image: url(https://static.igem.org/mediawiki/igem.org/0/02/Page_human_pratice_apple_app_relatedBar.png);<br />
background-repeat: repeat-x;<br />
background-position: top;<br />
padding: 12px;<br />
word-wrap: break-word;<br />
word-break: break-all;<br />
font-family: Arial, Helvetica, sans-serif;<br />
}<br />
.page_content_relatedBar_humanpractice_workshop {<br />
background-color: #465970;<br />
width: 283px;<br />
position: relative;<br />
margin-top: 20px;<br />
background-image: url(https://static.igem.org/mediawiki/igem.org/f/fc/Page_human_pratice_workshop_relatedBar.png);<br />
background-repeat: repeat-x;<br />
background-position: top;<br />
padding: 12px;<br />
word-wrap: break-word;<br />
word-break: break-all;<br />
font-family: Arial, Helvetica, sans-serif;<br />
}<br />
<br />
<br />
/*--------------various relatedBar------------*/<br />
.page_content_aboutus_textBar #page_content_textBar_enterTEXT ul {<br />
width: 620px;<br />
margin-top: 5px;<br />
margin-bottom: 50px;<br />
margin-left: 0px;<br />
float: left;<br />
}<br />
.page_content_aboutus_textBar #page_content_textBar_enterTEXT ul li {<br />
background-repeat: no-repeat;<br />
background-position: top;<br />
float: left;<br />
width: 114px;<br />
margin-right: 10px;<br />
margin-top: 20px;<br />
background-image: url(https://static.igem.org/mediawiki/igem.org/6/64/SYSU_Page_aboutus_photoBG.png);<br />
}<br />
.page_content_aboutus_textBar #page_content_textBar_enterTEXT ul p {<br />
font-family: Arial, Helvetica, sans-serif;<br />
color: #FFF;<br />
}<br />
.page_content_aboutus_textBar #page_content_textBar_enterTEXT ul h1 {<br />
font-family: Arial, Helvetica, sans-serif;<br />
font-weight: bold;<br />
color: #99ccff;<br />
}<br />
.page_content_aboutus_textBar #page_content_textBar_enterTEXT ul #fuking_tired {<br />
background-color: #292F2F;<br />
background-image: url(none);<br />
float: left;<br />
width: 600px;<br />
margin-top: 10px;<br />
}<br />
.page_content_aboutus_textBar #page_content_textBar_enterTEXT ul li p {<br />
font-family: Arial, Helvetica, sans-serif;<br />
color: #FFF;<br />
}<br />
.page_content_aboutus_textBar #page_content_textBar_enterTEXT {<br />
padding: 10px;<br />
float: left;<br />
width: 100%;<br />
margin-bottom: 50px;<br />
}<br />
.page_content_aboutus_textBar #page_content_textBar_enterTEXT ul li h1 {<br />
font-family: Arial, Helvetica, sans-serif;<br />
font-weight: bold;<br />
color: #99ccff;<br />
margin-top: 3px;<br />
margin-bottom: 5px;<br />
}<br />
.page_content_aboutus_textBar #page_content_textBar_enterTEXT ul #fuking_tired p {<br />
padding-left: 15px;<br />
}<br />
<br />
.page_content_text_Bar_enterTEXT_RpicBar {<br />
float: right;<br />
width: 180px;<br />
margin-left: 10px;<br />
}<br />
.page_content_textBar_enterTEXT_enter {<br />
float: left;<br />
width: 418px;<br />
}<br />
#background_shadow .page_WholeContainer #page_content_WholeBox .page_content_LsideBar .page_content_textBar #page_content_textBar_enterTEXT .page_content_textBar_enterTEXT_enter li {<br />
font-family: Arial, Helvetica, sans-serif;<br />
font-size: 14px;<br />
line-height: 1.5em;<br />
}<br />
/*-------------lightbox style---------------*/<br />
/**<br />
* jQuery lightBox plugin<br />
* This jQuery plugin was inspired and based on Lightbox 2 by Lokesh Dhakar (http://www.huddletogether.com/projects/lightbox2/)<br />
* and adapted to me for use like a plugin from jQuery.<br />
* @name jquery-lightbox-0.5.css<br />
* @author Leandro Vieira Pinho - http://leandrovieira.com<br />
* @version 0.5<br />
* @date April 11, 2008<br />
* @category jQuery plugin<br />
* @copyright (c) 2008 Leandro Vieira Pinho (leandrovieira.com)<br />
* @license CC Attribution-No Derivative Works 2.5 Brazil - http://creativecommons.org/licenses/by-nd/2.5/br/deed.en_US<br />
* @example Visit http://leandrovieira.com/projects/jquery/lightbox/ for more informations about this jQuery plugin<br />
*/<br />
#jquery-overlay {<br />
position: fixed;<br />
top: 0;<br />
left: 0;<br />
z-index: 90;<br />
width: 100%;<br />
height: 500px;<br />
}<br />
#jquery-lightbox {<br />
position: absolute;<br />
top: 0;<br />
left: 0;<br />
width: 100%;<br />
z-index: 100;<br />
text-align: center;<br />
line-height: 0;<br />
}<br />
#jquery-lightbox a img { border: none; }<br />
#lightbox-container-image-box {<br />
position: relative;<br />
background-color: #fff;<br />
width: 250px;<br />
height: 250px;<br />
margin: 0 auto;<br />
}<br />
#lightbox-container-image { padding: 10px; }<br />
<br />
#lightbox-loading {<br />
position: absolute;<br />
top: 40%;<br />
left: 0%;<br />
height: 25%;<br />
width: 100%;<br />
text-align: center;<br />
line-height: 0;<br />
}<br />
#lightbox-nav {<br />
position: absolute;<br />
top: 0;<br />
left: 0;<br />
height: 100%;<br />
width: 100%;<br />
z-index: 10;<br />
}<br />
#lightbox-container-image-box > #lightbox-nav { left: 0; }<br />
#lightbox-container-image-box.loaded {<br />
border-bottom: 0;<br />
}<br />
#lightbox-nav a { outline: none;}<br />
#lightbox-nav-btnPrev, #lightbox-nav-btnNext {<br />
width: 49%;<br />
height: 100%;<br />
zoom: 1;<br />
display: block;<br />
}<br />
#lightbox-nav-btnPrev { <br />
left: 0; <br />
float: left;<br />
}<br />
#lightbox-nav-btnNext { <br />
right: 0; <br />
float: right;<br />
}<br />
#lightbox-container-image-data-box {<br />
font: 10px Verdana, Helvetica, sans-serif;<br />
background-color: #fff;<br />
margin: 0 auto;<br />
line-height: 1.4em;<br />
overflow: auto;<br />
width: 100%;<br />
padding: 0 10px 0;<br />
}<br />
<br />
#lightbox-container-image-data {<br />
padding: 0 10px; <br />
color: #666; <br />
}<br />
#lightbox-container-image-data #lightbox-image-details { <br />
width: 70%; <br />
float: left; <br />
text-align: left; <br />
} <br />
#lightbox-image-details-caption { font-weight: bold; }<br />
#lightbox-image-details-currentNumber {<br />
display: block; <br />
clear: left; <br />
padding-bottom: 1.0em; <br />
} <br />
#lightbox-secNav-btnClose {<br />
width: 66px; <br />
float: right;<br />
padding-bottom: 0.7em; <br />
}<br />
<br />
@charset "utf-8";<br />
/* CSS Document */<br />
/* jQuery lightBox plugin - Gallery style */<br />
/* This layout is for sample purposes<br />
Feel free to edit it to suit your needs<br />
*/<br />
.lbGallery {<br />
background-color: #444;<br />
padding: 10px;<br />
width: 520px;<br />
}<br />
.lbGallery ul { list-style: none; }<br />
.lbGallery ul li { display: inline; }<br />
.lbGallery ul img {<br />
border: 5px solid #3e3e3e;<br />
border-width: 5px 5px 20px;<br />
}<br />
.lbGallery ul a:hover img {<br />
border: 5px solid #fff;<br />
border-width: 5px 5px 20px;<br />
color: #fff;<br />
}<br />
.lbGallery ul a:hover { color: #fff; }<br />
<br />
<br />
<br />
/*-------------------lightbox style-------------*/<br />
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<h2>SAFETY AND SECURITY QUESTIONS</h2><br />
<p>&nbsp;</p><br />
<h1>1. Would the materials used in your project and/or your final product pose: </h1><br />
<p>a. Risks to the safety and health of team members or others in the lab? </P><br />
<p>b. Risks to the safety and health of the general public if released by design or accident? </P><br />
<p>c. Risks to environmental quality if released by design or accident? </P><br />
<p>d. Risks to security through malicious misuse by individuals, groups or states? </P><br />
<p>Please explain your responses (whether yes or no) to these questions. </P><br />
<p>Specifically, are any parts or devices in your project associated with (or known to cause): </P> <br />
<p>- pathogenicity, infectivity, or toxicity? </P> <br />
<p>- threats to environmental quality? </P><br />
<p>- security concerns? </P> <br /><br />
<br />
<p>&nbsp;&nbsp;&nbsp;&nbsp;From the aspect of the project itself, no materials used by the iGEM 2011 SYSU-China or any final product would raise any safety issue in terms of researchers, the public or the environment. </P> <br /><br />
<p>&nbsp;&nbsp;&nbsp;&nbsp;First, the bacteria we are working on are Escherichia coli BL21 and DH5α,the non-virulent mutants of Escherichia coli, which are considered to be non-pathogenic and unlikely to survive in host tissues and cause disease. These are confirmed by R.M. La Ragione and M.J. Woodwad in their paper An investigation into the pathogenic properties of Escherichia coli strains BLR, BL21, DH5a and EQ1 (http://ors.uchc.edu/bio/resources/pdf/3.6.1.A_colipath.pdf). </P> <br /><br />
<p>&nbsp;&nbsp;&nbsp;&nbsp;Second, since several years' operation of the two kinds of E.coli in the labs and no virulence against human has been reported, we believe that these strains are harmless to team members or others in the lab. Except to that, team members or others in the lab are protected appropriately, such as wearing gloves and masks, and this also reduces the risks that they are infected or get other hurts in the lab. </P> <br /><br />
<p>&nbsp;&nbsp;&nbsp;&nbsp;Third, even if our bacteria are released from the lab by design or accident, they will not pose any risk to the safety and health of the general public, because as we mentioned above, they are the non-virulent mutants of Escherichia coli, and are non-pathogenic and unlikely to survive in host tissues and cause disease. As a result, they are safe to the general public. In terms of the environment, our bacteria are difficult to survive in the natural environment, because they need enough nutrition in which the compositions are in accordance with an appropriate proportion. Since they are difficult to survive, they are unlikely to cause safety problems to the environment. As for the malicious misuse of these bacteria, it is difficult to get the guarantee that they are doomed to be safe. Basically, as long as they are not transformed maliciously, they will not be able to cause the safety issues even if they are maliciously misused. </P> <br /><br />
<br />
<h1>2. Do any of the new BioBrick parts (or devices) that you made this year raise any safety issues? If yes, </h1><br />
<p>a. Did you document these issues in the Registry? </P><br />
<p>b. How did you manage to handle the safety issue? </P><br />
<p>c. How could other teams learn from your experience? </P><br /><br />
<br />
<p>&nbsp;&nbsp;&nbsp;&nbsp;No biobricks made by the SYSU-China team will raise any safety issues. The biobricks, such as recA, recN, cheZ, trkD and ag43, are all the natural parts of E.coli in natural environment, and commonly used for laboratory operation. Their function and functional mechanism are clearly known, and they are not associated with pathogenicity, infectivity, or toxicity, nor will they cause threats to environmental quality. Moreover, cheZ and ag43 are standard biobricks provided by iGEM authority, so the safety of these biobricks can be guaranteed. </P> <br /><br />
<br />
<h1>3. Under what biosafety provisions will / do you operate? </h1><br />
<p>a. Does your institution have its own biosafety rules and if so what are they? Provide a link to them online if possible. </P><br />
<p>b. Does your institution have an Institutional Biosafety Committee or equivalent group? If yes, have you discussed your project with them? Describe any concerns or changes that were made based on this review. </P><br />
<p>c. Will / did you receive any biosafety and/or lab training before beginning your project? If so, describe this training. </P><br />
<p>d. Does your country have national biosafety regulations or guidelines? If so, provide a link to them online if possible. </P> <br /><br />
<br />
<p>&nbsp;&nbsp;&nbsp;&nbsp;First, the SYSU_China team members conform strictly to the established safety rules in the lab (http://biosafety.sysu.edu.cn/administer/UploadFiles_9471/200804/2008041817410158.pdf). </P> <br /><br />
<p>&nbsp;&nbsp;&nbsp;&nbsp;Second, In our university, Biosafety Committee of Sun Yat-sen University is responsible for monitoring the safety of all the research activities on the campus. Their regulations are stipulated according to the WHO Laboratory biosafety manual, which is also conformed to by our country (http://biosafety.sysu.edu.cn/Soft/UploadSoft/200804/2008042116071397.pdf). Before our team started the program, we have talked about the safety issues of the whole project. They were really concerned about the radiation-related experiments, and emphasized that these experiments must be operated by the professional technician. In addition to that, they also requested that all procedures conducted in this project should be performed according to the rules stipulated by WHO. On the whole, they think about our project as safe. With the surveillance of the Biosafety Committee of Sun Yat-sen University and the cautious operation of our members, the project will be harmless to the researchers, the public or the environment. </P> <br /><br />
<p>&nbsp;&nbsp;&nbsp;&nbsp;Third, each team member was required to attend a pre-lab training led by graduate students and advisors both on experimental skills and safety instructions before he or she actually started to do the iGEM program in the lab. They trained us how to operate the fundamental experiments, and taught us the basic safety rules in the lab, such as how to use the toxic reagents. During the program, we wear gloves properly and disinfect tubes and plates after use. When it involves the radiation-related experiments, the operation will be carried out by the technician with radiation-usage permission. </P> <br /><br />
<br />
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<h1>4. OPTIONAL QUESTION: Do you have other ideas on how to deal with safety or security issues that could be useful for future iGEM competitions? How could parts, devices and systems be made even safer through biosafety engineering? </h1> <br /><br />
<br />
<p>&nbsp;&nbsp;&nbsp;&nbsp;We suggest that the safety information of any part used in the iGEM competition, especially its functions, should be investigated clearly through literature search before the usage. If the part has any intention to raise safety issues, the iGEM team should refuse to use it and choose other alternatives. In addition to that, iGEM teams should submit the safety report with their biobricks part to the iGEM authority, describing the safety issues of the biobricks part, which should also be examined by a professional third party. Thus the high-risk biobricks or parts with potential biosafety problems will be identified and abandoned before submitting. Through this way can the parts, devices and systems be made much safer. With above measures, the safety of the biobricks submitted can be guaranteed. </P> <br /><br />
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</div><br />
</div><br />
<!---------------------------------------------!--><br />
<div class="page_content_RsideBar"><br />
<div class="page_content_videoBar"><br />
<h1>Please watch our animination</h1><br />
<iframe width="307" height="225" src="http://www.youtube.com/embed/KymdcolAhcE?rel=0" frameborder="0" allowfullscreen></iframe><br />
<br />
<br/><br />
<br />
</div><br />
<div class="page_content_relatedBar_project_sf"><br />
<h2>Pages we think helpful:</h2><br />
<br />
<h1><a href="https://2011.igem.org/Team:SYSU-China/page_project_modules_construction">see how we construct our modules</a></h1><br />
<h1><a href="https://2011.igem.org/Team:SYSU-China/page_project_modules_verification">see how we verify each module</a></h1><br />
<h1><a href="https://2011.igem.org/Team:SYSU-China/main_page_story">or see our whole idea in Story page</a></h1><br />
<p>&nbsp;</p><br />
</div><br />
</div> <br />
<!---------------------------------------------!--><br />
<div class="main_page_clearer_light"></div><br />
<!---------------------------------------------!--> <br />
<br />
</div><!--here ends the page_content_WholeBox!--><br />
<div class="main_page_clearer_light"></div><br />
</div><!--here ends the mainpage_project_WholeContainer!--><br />
<br />
<!--here begins the contact_bottombar!--><br />
<div class="contact_bottombar"><br />
<p>From the 2011 iGEM team SYSU-China (2011)</p><br />
<p>Sun Yat-Sen University, Guangzhou, China</p><br />
<p>Address: 135# Xingang Rd.(W.), Haizhu Guangzhou, P.R.China</p><br />
<p><a href="http://eng.sysu.edu.cn/">visit the Sun Yat-sen university website</a></p><br />
<p>Thanks Apycom jQuery Menus and <a href="http://apycom.com/">visit their website</a><br />
</div><br />
<!--here ends the contact_bottombar!--><br />
<br />
</div><!--here ends the backgroud_shadow_bar!--><br />
</body><br />
</html></div>Cambihttp://2011.igem.org/Team:SYSU-China/page_project_datapageTeam:SYSU-China/page project datapage2011-10-06T02:30:43Z<p>Cambi: </p>
<hr />
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<html xmlns="http://www.w3.org/1999/xhtml"><br />
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<script type="text/javascript" language="javascript"><br />
$(function(){<br />
// $("#page_content_baBar_details").scrollTo(600,2横向)<br />
<br />
//<br />
// <br />
$("#page_content_baBar_details").scrollTo(700)<br />
});</script><br />
</head><br />
<br />
<body><br />
<div id="background_shadow"><br />
<div class="page_WholeContainer"> <br />
<br />
<!--------here begins the manu bar section--!--><br />
<div class="title_container"><br />
<div id="menu"><br />
<ul class="menu"><br />
<li><a href="index.thml"><span></span></a></li><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/main_page_news"><span>NEWS</span></a></li><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/main_page_story"><span>STORY</span></a></li> <br />
<li><a href="https://2011.igem.org/Team:SYSU-China/main_page_project" class="parent"><span>PROJECT</span></a><br />
<div><br />
<ul><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/page_project_modules_construction"><span>Modules Construction</span></a><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/page_project_modules_verification"><span>Modules Verification</span></a></li><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/page_project_functional_construction"><span>Functional Verification</span></a></li><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/page_project_notes"><span>Notes</span></a></li><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/page_project_datapage"><span>Date Page</span></a></li><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/page_project_safety"><span>Safety</span></a></li><br />
</ul><br />
</div><br />
</li><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/main_page_human_practice" class="parent"><span>HUMAN PRACTICE</span></a><br />
<div><br />
<ul><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/page_human_practice_app"><span>App</span></a><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/page_human_practice_labcraft"><span>LabCraft Board Game</span></a></li><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/page_human_practice_workshop"><span>Workshop</span></a></li><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/page_human_practice_survey"><span>Survey</span></a></li><br />
</ul><br />
</div><br />
</li><br />
<br />
<li><a href="https://2011.igem.org/Team:SYSU-China/main_page_aboutus" class="parent"><span>ABOUT US</span></a><br />
<div><br />
<ul><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/page_aboutus_team_members"><span>Team Members</span></a><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/page_aboutus_special_thanks"><span>Spcial Thanks</span></a></li><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/page_aboutus_LT"><span>Logo and T-shirt</span></a></li><br />
</ul><br />
</div><br />
</li><br />
<br />
</ul><br />
</div><!--here ends the manu!--><br />
</div><!--here ends the title_container!--><br />
<div id="logo_bar"></div> <br />
<!--------here ends the title_section------!--><br />
<br />
<div class="page_clear_and_introBar"><br />
<img src="https://static.igem.org/mediawiki/igem.org/5/57/SYSU_Page_project_data_page_introBar.png<br />
" width="958" height="65" /></div><br />
<!------here begins the main content section--!--><br />
<br />
<div id="page_content_WholeBox"><br />
<!---------------------------------------------!--><br />
<div class="page_content_LsideBar"><br />
<div class="page_content_bgBar"><img src="https://static.igem.org/mediawiki/igem.org/0/0d/SYSU_Page_project_data_page_bg.jpg<br />
" width="632" height="600" /><br />
<div id="page_content_baBar_details"><a href="#page_content_textBar_enterTEXT">See Details</a></div><br />
</div><br />
<div class="page_content_textBar"><br />
<div id="page_content_textBar_enterTEXT"><br />
<br />
<p>See SYSU-China 2011 iGEM Team Parts<br />
<p>&nbsp; </p><br />
<p><a href="http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2011&amp;group=SYSU-China">http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2011&amp;group=SYSU-China</a></p><br />
<br />
</div> <br />
</div><br />
</div><br />
<!---------------------------------------------!--><br />
<div class="page_content_RsideBar"><br />
<div class="page_content_videoBar"><br />
<h1>Please watch our animination</h1><br />
<iframe width="307" height="225" src="http://www.youtube.com/embed/KymdcolAhcE?rel=0" frameborder="0" allowfullscreen></iframe><br />
<br />
<br/><br />
<br />
</div><br />
<div class="page_content_relatedBar_project_dp"><br />
<h2>Pages we think helpful:</h2><br />
<h1><a href="https://2011.igem.org/Team:SYSU-China/page_project_modules_construction">see how we construct our modules</a></h1><br />
<h1><a href="https://2011.igem.org/Team:SYSU-China/page_project_modules_verification">see how we verify each module</a></h1><br />
<h1><a href="https://2011.igem.org/Team:SYSU-China/main_page_story">or see our whole idea in Story page</a></h1><br />
</div><br />
</div> <br />
<!---------------------------------------------!--><br />
<div class="main_page_clearer_light"></div><br />
<!---------------------------------------------!--> <br />
<br />
</div><!--here ends the page_content_WholeBox!--><br />
<div class="main_page_clearer_light"></div><br />
</div><!--here ends the mainpage_project_WholeContainer!--><br />
<br />
<!--here begins the contact_bottombar!--><br />
<div class="contact_bottombar"><br />
<p>From the 2011 iGEM team SYSU-China (2011)</p><br />
<p>Sun Yat-Sen University, Guangzhou, China</p><br />
<p>Address: 135# Xingang Rd.(W.), Haizhu Guangzhou, P.R.China</p><br />
<p><a href="http://eng.sysu.edu.cn/">visit the Sun Yat-sen university website</a></p><br />
<p>Thanks Apycom jQuery Menus and <a href="http://apycom.com/">visit their website</a><br />
</div><br />
<!--here ends the contact_bottombar!--><br />
<br />
</div><!--here ends the backgroud_shadow_bar!--><br />
</body><br />
</html></div>Cambihttp://2011.igem.org/Team:SYSU-China/page_project_notesTeam:SYSU-China/page project notes2011-10-06T02:29:48Z<p>Cambi: </p>
<hr />
<div>{{:Team:SYSU-China/header/temp}}<br />
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<title>Notes-Sun Yat-sen Univ.</title><br />
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<script type="text/javascript" src="menu.js"></script><br />
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<script type="text/javascript" language="javascript"><br />
$(function(){<br />
// $("#page_content_baBar_details").scrollTo(600,2横向)<br />
<br />
//<br />
// <br />
$("#page_content_baBar_details").scrollTo(700)<br />
});</script><br />
<br />
<script src="SpryDOMUtils.js" type="text/javascript"></script><br />
<script src="SpryDOMEffects.js" type="text/javascript"></script><br />
<script src="SpryWidget.js" type="text/javascript"></script><br />
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<br />
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<link href="SpryTabbedPanels2.css" rel="stylesheet" type="text/css" /><br />
<style type="text/css"><br />
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<body><br />
<div id="background_shadow"><br />
<div class="page_WholeContainer"> <br />
<br />
<!--------here begins the manu bar section--!--><br />
<div class="title_container"><br />
<div id="menu"><br />
<ul class="menu"><br />
<li><a href="index.thml"><span></span></a></li><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/main_page_news"><span>NEWS</span></a></li><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/main_page_story"><span>STORY</span></a></li> <br />
<li><a href="https://2011.igem.org/Team:SYSU-China/main_page_project" class="parent"><span>PROJECT</span></a><br />
<div><br />
<ul><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/page_project_modules_construction"><span>Modules Construction</span></a><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/page_project_modules_verification"><span>Modules Verification</span></a></li><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/page_project_functional_construction"><span>Functional Verification</span></a></li><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/page_project_notes"><span>Notes</span></a></li><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/page_project_datapage"><span>Date Page</span></a></li><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/page_project_safety"><span>Safety</span></a></li><br />
</ul><br />
</div><br />
</li><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/main_page_human_practice" class="parent"><span>HUMAN PRACTICE</span></a><br />
<div><br />
<ul><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/page_human_practice_app"><span>App</span></a><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/page_human_practice_labcraft"><span>LabCraft Board Game</span></a></li><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/page_human_practice_workshop"><span>Workshop</span></a></li><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/page_human_practice_survey"><span>Survey</span></a></li><br />
</ul><br />
</div><br />
</li><br />
<br />
<li><a href="https://2011.igem.org/Team:SYSU-China/main_page_aboutus" class="parent"><span>ABOUT US</span></a><br />
<div><br />
<ul><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/page_aboutus_team_members"><span>Team Members</span></a><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/page_aboutus_special_thanks"><span>Spcial Thanks</span></a></li><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/page_aboutus_LT"><span>Logo and T-shirt</span></a></li><br />
</ul><br />
</div><br />
</li><br />
<br />
</ul><br />
</div><!--here ends the manu!--><br />
</div><!--here ends the title_container!--><br />
<div id="logo_bar"></div> <br />
<!--------here ends the title_section------!--><br />
<br />
<div class="page_clear_and_introBar"><br />
<img src="https://static.igem.org/mediawiki/igem.org/2/2c/SYSU_Page_project_notes_introBar.png<br />
" width="958" height="65" /></div><br />
<!------here begins the main content section--!--><br />
<br />
<div id="page_content_WholeBox"><br />
<!---------------------------------------------!--><br />
<div class="page_content_LsideBar"><br />
<div class="page_content_bgBar"><img src="https://static.igem.org/mediawiki/igem.org/c/c2/SYSU_Page_project_notes_bg.jpg<br />
" width="632" height="600" /><br />
<div id="page_content_baBar_details"><a href="#TabbedPanels2">See Details</a></div><br />
</div><br />
<div class="page_content_textBar"><br />
<div id="TabbedPanels2"><br />
<h2>Experiments Dairy</h2><br />
<br /><br /><br />
<li>July.8, 2011 Friday</li><br />
<li>Xu Yitian, Sun Weiwen, Zhao Zhilun: </li><br />
<p>Inoculate 10mL LB with the E. Coli BL21 plys strain.</P><br />
<br /><br />
<li>July.9, 2011 Saturday</li><br />
<li>Xu Yitian, Sun Weiwen, Zhao Zhilun: </li> <br />
<p>Preserve the E. Coli BL21 plys strain in 4℃ incubator. </P><br />
<br /><br />
<li>July.11, 2011 Monday</li><br />
<li>Xu Yitian, Sun Weiwen, Zhao Zhilun: </li> <br />
<p>1. Temperature gradient PCR of the two genes, trkD and cheZ, and two promoters, recA and recN promoters, according to their primers' Tm. </P> <br />
<br /><br />
<p>2. Result: The best PCR annealing temperature of trkD and cheZ are 60°and 45°respectively. The PCR of recA and recN promoters will be repeated tomorrow. </P> <br /><br />
<br />
<li>July.12, 2011 Tuesday</li><br />
<li>Xu Yitian, Sun Weiwen, Zhao Zhilun: </li> <br />
<p>1. PCR of trkD and cheZ to get large amount of these genes, but failed because of mistake of wrong PCR system. </P> <br /><br />
<p>2. PCR of recA and recN promoters, the best PCR temperature are both 60°. </P> <br /><br />
<p>3. Repeat the PCR of trkD and cheZ and extract pUC18 plasmid. Success!<br />
<li>July.13, 2011 Wednesday</li><br />
<li>Xu Yitian, Sun Weiwen, Zhao Zhilun: </li> <br />
<p>Cut the protective bases of the restriction sites of EcoRI and XbaI, and tried to link the promoters to the genes but failed. </P> <br /><br />
<li>July.16, 2011 Saturday</li><br />
<li>Xu Yitian, Sun Weiwen, Zhao Zhilun: </li> <br />
<p>Cut the plasmid with the enzyme of EcoRI, and link the promoters and genes and the plasmid together over night in 16°. </P> <br /><br />
<li>July.17, 2011 Sunday</li><br />
<li>Xu Yitian, Sun Weiwen, Zhao Zhilun: </li> <br />
<p>Eletrotransform the linked product to DH5a competence cells and revive with LB and culture them on the LB plate. However, there are no growing cells on the plate, which means our first try failed. </P> <br /><br />
<li>July.18, 2011 Monday</li><br />
<li>Xu Yitian, Sun Weiwen, Zhao Zhilun: </li> <br />
<p>PCR of the two promoters and the two genes, but during the gel extraction process, a small accident happened and the covers of the Ep tubes became powder, which blocked us from distinguish our four products. But we conducted a electrophoresis of our four products and we can differ our products. </P> <br /> <br />
<br />
<br />
<li>July.19, 2011 Tuesday</li><br />
<li>Xu Yitian, Sun Weiwen, Zhao Zhilun: </li> <br />
<p>Cut the protective bases of the restriction sites of EcoRI and XbaI, and cut the plasmid with the enzyme of EcoRI, and link the promoters and genes and the plasmid together over night in 16°. </P> <br /><br />
<li>July.20, 2011 Wednesday</li><br />
<li>Xu Yitian, Sun Weiwen, Zhao Zhilun: </li> <br />
<p>Eletrotransform the linked product to DH5a competence cells and revive with LB and culture them on the LB plate. However, there are no growing cells on the plate, which means our first try failed. </P> <br /><br />
<li>July.21, 2011 Thursday</li><br />
<li>Xu Yitian, Sun Weiwen, Zhao Zhilun: </li> <br />
<p>1. Use the PCR products two days ago and cut the restriction sites of them and linearize the plasmid, and link the promoters, the genes and the plasmid in 16° overnight. </P> <br /><br />
<p>2. Re-PCR recN since the first design of it has some mistake. </P> <br /><br />
<li>July.28, 2011 Thursday</li><br />
<li>Xu Yitian, Sun Weiwen, Zhao Zhilun: </li> <br />
<p>Successfully link recA promoter to the gene of cheZ (there are colonies on the plate and positive results of Conlony PCR). Then we sent the strain to Invitrogen to check its sequence if there is any base mutation. It turned out that the promoter and the gene are normal. Till now, we get our first useful plasmid: recA-cheZ-pUC18. </P> <br /><br />
<li>July.29, 2011 Friday</li><br />
<li>Xu Yitian, Sun Weiwen, Zhao Zhilun: </li> <br />
<p>We tried to construct the plasmid with GFP. We design the restriction sites of the GFP to be PstI and HindIII, and then we purified the GFP and linked it to the linearized pUC18. </P> <br /><br />
<br />
<li>July.30, 2011 Saturday</li><br />
<li>Xu Yitian, Sun Weiwen, Zhao Zhilun: </li> <br />
<p>1. Eletrotransform the linking product to DH5a competence cells, and grow them on plate. </P> <br /> <br />
<p>2. We continued to link recN to trkD. We PCR the two parts and cut them with EcoRI and XbaI, and cut the pUC18 plasmid with EcoRI. And links them at 16°overnight. </P> <br /><br />
<li>July.31, 2011 Sunday</li><br />
<li>Xu Yitian, Sun Weiwen, Zhao Zhilun: </li> <br />
<p>1. There is no colony on the plate. </P> <br /><br />
<p>2. Eletrotransform the linking product of recN and trkD to DH5a competence cells, and grow them on plate. </P> <br /> <br />
<li>Aug.1, 2011 Monday</li><br />
<li>Xu Yitian, Sun Weiwen, Zhao Zhilun: </li><br />
Colony PCR of the colonies on the plate of recN-trkD, however, we use the wrong primers and get no result. We corrected the mistake and get 4 positive results. We inoculate 2 conlonies into 5mL LB. </P> <br /><br />
<li>Aug.2, 2011 Tuesday</li><br />
<li>Xu Yitian, Sun Weiwen, Zhao Zhilun: </li><br />
<p>We sent the two strains to check sequence. </P> <br /><br />
<li>Aug.7, 2011 Sunday</li><br />
<li>Xu Yitian, Sun Weiwen, Zhao Zhilun: </li><br />
<p>The recN and trkD did not link into the plasmid by checking the sequencing result. </P> <br /><br />
<li>Aug.8, 2011 Monday</li><br />
<li>Xu Yitian, Sun Weiwen, Zhao Zhilun: </li><br />
<p>1. We ran a temperature gradient PCR of the new cheZ and trkD, both of which are without terminator because we want to link the two genes before the GFP and exam the gene expression by observe the fluorescence of GFP. However, the PCR of trkD(no terminator) failed while the cheZ(no termination ) succeed. </P> <br /> <br />
<p>2. Cut the plasmid of pUC18 with HindIII and PstI, then exam the product with the GFP gene. Then link the gene of GFP with the plasmid of pUC18. </P> <br /><br />
<br />
<br />
<li>Aug.9, 2011 Tuesday</li><br />
<li>Xu Yitian, Sun Weiwen, Zhao Zhilun: </li><br />
<p>Eletrotransform the linking product into DH5a cells and culture them on an Amp plate. Patch again after 12h culture. </P> <br /> <br />
<li>Aug.10, 2011 Wednesday</li><br />
<li>Xu Yitian, Sun Weiwen, Zhao Zhilun: </li><br />
<p>1. Colony PCR of the colonies on the plate of GFP-pUC18 and get 3 positive results. Inoculate 3 colonies into 5mL LB and place them in the shaker of 220rpm 37°overnight. </P> <br /> <br />
<p>2. PCR of CheZ and trkD, both of which are off terminators, and the two promoters. Then use enzymes to cut the protective sites of the genes and the restriction sites of the plasmid of GFP-pUC18. Then link recA, recN, cheZ, trkD respectively with the plasmid. </P> <br /> <br />
<br />
<li>Wang Zilong, Wang Li, Zheng Yi: </li><br />
<p>1. Double digest pUC18 and EGFP with HindIII and PstI, then ligate pUC18 and EGFP with T4 ligase in 16℃ over night. (1 is 1Kb marker, 2 is pUC18 and 3 is EGFP). </P> <br /><br />
<br />
<p>2. Gradient PCR to search the proper condition of cloning trkD and cheZ (for western blot). (Left side is cheZ, right side is trkD). </P> <br /><br />
<br />
<br />
<br />
<li>Aug.11, 2011 Thursday</li><br />
<li>Xu Yitian, Sun Weiwen, Zhao Zhilun: </li><br />
<p>1. Send the 3 GFP-pUC18 strains to Invitrogen to check their sequence. And we extract plasmid from the leaved inocula. </P> <br /><br />
<p>2. Eletrotransform the linking product into DH5a cells and culture them on Amp plates. Patch again after 12h culture. </P> <br /><br />
<br />
<li>Wang Zilong, Wang Li, Zheng Yi: </li><br />
<p>1. Transformation of pUC18-EGFP to DH5α. </P> <br /><br />
<p>2. Use KOD plus polymerase to amplifying trkD and cheZ (for western blot). </P> <br /><br />
<br />
<p>3. Double digest trkD and cheZ (for western blot) with EcoRI and HindIII, then ligate them to pET-28a and pET-32a which were digested with same enzymes. However, with few units of plasmids, this ligation had to be done in next day. </P> <br /><br />
<br />
<li>Aug. 12, 2011 Friday</li><br />
<li>Wang Zilong, Wang Li, Zheng Yi: </li><br />
<p>1. Re-digest pET-28a and pET-32a with EcoRI and HindIII, then ligate to trkD and cheZ (for western blot) with T4 ligase in 16℃ over night. (1 is cheZ, 2 is trkD, 3 is 1Kb marker, 4 is pET28a, 5 is pET-32a). </P> <br /><br />
<br />
<p>2. PCR trkD (for recombinant PCR), but failed. </P> <br /><br />
<p>3. Clony PCR pUC18-EGFP with Go Taq® to test the result of transformation. </P> <br /><br />
<br />
<br />
<li>Aug.14, 2011 Sunday</li><br />
<li>Xu Yitian, Sun Weiwen, Zhao Zhilun: </li><br />
<p>Patch the colonies on the plates and run a colony PCR and got several positive result .Inoculate the positive strain with 5mL LB respectively. </P> <br /><br />
<li>Aug.15, 2011 Monday</li><br />
<li>Xu Yitian, Sun Weiwen, Zhao Zhilun: </li><br />
<p>Send our samples to check their sequence. Then extract plasmids from those different strains. </P> <br /><br />
<li>Aug.16, 2011 Tuesday</li><br />
<li>Xu Yitian, Sun Weiwen, Zhao Zhilun: </li><br />
<p>Cut the different plasmids and exam whether these plasmids have genes in them. The cheZ-GFP-pUC18 and trkD-GFP-pUC18 do have the two genes on the plasmid, while the two promoters failed. </P> <br /> <br />
<br />
<li>Wang Zilong, Wang Li, Zheng Yi: </li><br />
<p>Try to use recombinant PCR to clone recN-trkD in one-step program and two-step program with Go Taq®, which helps to find the proper condition of PCR. </P> <br /><br />
<br />
<li>Aug. 17, 2011 Wednesday</li><br />
<li>Wang Zilong, Wang Li, Zheng Yi: </li><br />
<p>1. Digest plasimds cheZ-pET-32a and trkd-pET-32a with EcoRI and double digest with EcoRI and HindIII to test whether their lengths are correct, and the result shows trkd-pET-32a is correctly, but cheZ-pET-32a is not. </P> <br /><br />
<br />
<p>2. Try to use recombinant PCR to clone recN-trkD in two-step program with Go Taq®, which helps to find the proper condition of PCR. </P> <br /><br />
<br />
<li>Aug. 19, 2011 Friday</li><br />
<li>Wang Zilong, Wang Li, Zheng Yi: </li><br />
<p>Induce cheZ-pET-32a and trkd-pET-32a with 0.1, 0.5, 1.0 mM IPTG in OD 0.600. </P> <br /><br />
<li>Aug. 20, 2011 Saturday</li><br />
<li>Wang Zilong, Wang Li, Zheng Yi: </li><br />
<p>In SDS-PAGE, use proteins in liquid and lysate expressed by cheZ-pET-32a and trkd-pET-32a to do electrophoresis. And the gel with proteins in liquid does western blot, while the other gel is dyed with G250. (1~4 are trkd-pET-32a induced by 0, 0.1, 0.5, 1.0 mM IPTG, 5 is marker, 6~9 are cheZ-pET-32a induced by 0, 0.1, 0.5, 1.0 mM IPTG). </P> <br /><br />
<br />
<li>Aug.21, 2011 Sunday</li><br />
<li>Xu Yitian, Sun Weiwen, Zhao Zhilun: </li><br />
<p>We inoculated the cheZ-GFP-pUC18 and trkD-GFP-pUC18 with 5mL LB. We use IPTG to trigger the lac promoter before both the cheZ-GFP-pUC18 and trkD-GFP-pUC18, then use fluorescence microscope to exam whether the two genes have successfully expressed. </P> <br /> <br />
<li>Aug.22, 2011 Monday</li><br />
<li>Xu Yitian, Sun Weiwen, Zhao Zhilun: </li><br />
<p>We intermediatecultured 1ml strains with 100mL LB. After two hours shaking and measured the OD value of the two strains of 0.45, we separated the 100ml inocula into 4 conical flasks and induced with 3 different concentrations of IPTG(one leaved is control). After 4 hours induction, we used fluorescence microscope to exam the inocula and find out that the control group, which should not be of a dark view, have fluorescence too. That means the lac promoter can trigger the expression of its downstream genes without induction. Then we want to transfer our genes onto another plasmid, pET28a, whose promoter lied between the restriction sites of BamHI and BglII. The two enzymes are isocaudamers and the gap cut by them can be linked without inserting any sequence. </P> <br /> <br />
<br />
<li>Wang Zilong, Wang Li, Zheng Yi: </li><br />
<p>Clone recN-trkD recombination in recombinant PCR program with PrimeStar®. After double digest the gene with EcoRI and PstI, ligate it with plasmid pUC18-EGFP digested with same enzymes. (1 is 1Kb marker, 2 is recN-trkD, 3 is pUC18-EGFP). </P> <br /><br />
<br />
<li>Aug.23, 2011 Tuesday</li><br />
<li>Xu Yitian, Sun Weiwen, Zhao Zhilun: </li><br />
<p>1. Cut the plasmid of GFP-pUC18 and the protective sites of recN and recA with the enzyme of XbaI and EcoRI, but the plasmid is so little amount that it cannot be seen on the gel. Inoculate the strain with the plasmid of GFP-pUC18. </P> <br /><br />
<p>2. Use BamHI and BglII to cut the plasmid of pET28a, and use T4 ligation enzyme to link the gap cut by the two enzymes of the plasmid. </P> <br /><br />
<li>Aug.24, 2011 Wednesday</li><br />
<li>Xu Yitian, Sun Weiwen, Zhao Zhilun: </li><br />
<p>1. Eletrotransform the linking product into DH5a cells and culture them on Amp plates. Patch again after 12h culture. Wait 12h but no colony found. </P> <br /><br />
<p>2. Extract plasmid from the inocula. recut the plasmid and get a clear line. Then try to link recA and recN onto the plasmid. 16°overnight. </P> <br /><br />
<li>Aug.25, 2011 Thursday</li><br />
<li>Xu Yitian, Sun Weiwen, Zhao Zhilun: </li><br />
<p>1. Repeat the experiment of pET28a promoter cutting. </P> <br /><br />
<p>2. Eletrotransform the linking product into DH5a cells and culture them on Amp plates. Patch again after 12h culture. </P> <br /> <br />
<li>Aug.26, 2011 Friday</li><br />
<li>Xu Yitian: </li><br />
<p>NO COLONY FOUND!!!!! </P> <br /><br />
<li>Sept.1, 2011 Thursday</li><br />
<li>Wang Li: </li><br />
<p>Western Blot</P> <br /><br />
<br />
<li>Sept.6, 2011 Tuesday</li><br />
<li>Sun Weiwen: </li><br />
<p>1. Transfer the pET32 plasmid into E.coli BL21. </P> <br /><br />
<p>2. Culture the pET32a-BL21 E.coli and the trkD-pET32a-BL21 E.coli with 5ml LB medium. </P> <br /><br />
<p>3. Put 3ml culture of the pET32a-BL21 E.coli and the trkD-pET32a-BL21 E.coli into 100ml CsCl-LB solution (CsCl content: 0.4g/100ml LB) respectively. Two hours later, their respective OD values are 0.35 and 0.53. Then induce them with 500μl IPTG(concentration: 0.1mol/L). Four and a half hours later, their respective OD values are 1.03 and 1.14. Then keep them still in the 37℃ incubator for 4 hours. Centrifugate the culture to collect the E.coli, then wash them with PBS for two times. In the end, suspend the E.coli with 20ml ddH2O respectively. Then send the two samples of the pET32a-BL21 E.coli and the trkD-pET32a-BL21 E.coli to China National Analytical Center, Guangzhou for the analysis of Cs element. </P> <br /><br />
<br />
<li>Wang Li: </li><br />
<p>Western Blot</P> <br /><br />
<br />
<li>Sept. 7, 2011 Wednesday</li><br />
<li>Wang Zilong, Zheng Yi: </li><br />
<p>1. Transform pSB1C3, pSB1A3, pSB1T3 and BBa_J04450 to DH5α. </P> <br /><br />
<p>2. Transform cheZ-pET-28a to strains whose cheZ gene is knocked out, and smear them on the medium with IPTG line to test chemotaxis. </P> <br /><br />
<li>Zhao Zhilun: </li><br />
<p>1. Double digest gfp-pUC18 and recA-cheZ(without terminator) with EcoRI and PstI in the 30μl system. Ligate gfp-pUC18 with recA-cheZ(without terminator) overnight. Electrotransform the ligation product into E.coli DH5α. Smear them on the solid medium containing Ampicillin. </P> <br /> <br />
<p>2. Double digest pET28a with BamHI and BglII, and then ligate itself with T4 ligase overnight.<br />
<p>3. Use colony PCR to detect whether recA-gfp-pUC18-infusion is successfully constructed. The result indicates that it fails. </P> <br /><br />
<p>4. Sequence the only grown E.coli in yesterday which contain recA-gfp-pUC18-infusion. </P> <br /> <br />
<li>Sept. 8, 2011 Thursday</li><br />
<li>Wang Zilong, Zheng Yi: </li><br />
<p>Do PCR on recN and trkD (for biobrick assembling) with KOD plus® polymerase, but failed. </P> <br /><br />
<br />
<li>Zhao Zhilun: </li><br />
<p>1. Electrotransform the self-ligation product of pET28a into DH5α, and smear them on the solid medium. </P> <br /><br />
<p>2. Result of colony PCR shows that I fail to construct recA-GFP-pUC18 in-fusion. </P> <br /><br />
<li>Sept. 9, 2011 Friday</li><br />
<li>Wang Zilong, Zheng Yi: </li><br />
<p>1. Do gradient PCR to clone recN and trkD (for biobrick assembling) with Go Taq®, but failed again. </P> <br /><br />
<p>2. Try to use cheZ-pET-28a to test chemotaxis again. </P> <br /><br />
<br />
<li>Wang Li: </li><br />
<p>Western Blot</P> <br /><br />
<br />
<li>Sept.10, 2011 Saturday</li><br />
<li>Sun Weiwen: </li><br />
<p>1. Culture the E.coli with cheZ-gfp-pUC18 and trkD-gfp-pUC18 with 5ml LB medium overnight. </P> <br /><br />
<p>2. Expand the culture with 100ml LB medium containing ampicillin. Two hours later, both of their OD values are 0.54. Then divide the 100ml culture into two bottles of 50ml, and induce one of the two with IPTG, keeping the other as the control. Four hours later, observe the expression of EGFP with confocal laser scanning microscopy. The result is that the experiment group is brighter than the control group. </P> <br /><br />
<br />
<li>Wang Zilong, Zheng Yi: </li><br />
<p>1. Use Go Taq® to PCR recN again, but failed again. </P> <br /><br />
<p>2. Collect plasmid pSB1C3 to test the length of it. </P> <br /><br />
<li>Sept. 11, 2011 Sunday</li><br />
<li>Wang Zilong, Zheng Yi: </li><br />
<p>1. Digest plasmid pSB1C3 with EcoRI, SpeI, XbaI and PstI, respectively, to test the plasmid. (1 and 6 is 1Kb marker, 2~5 are pSB1C3 digested by EcoRI, SpeI, XbaI and PstI, respectively). </P> <br /><br />
<br />
<p>2. Use new template bacterial to PCR recN and trkD (for biobrick assembling) with Go Taq®, and succeed in time; then amplifying cloning the genes. </P> <br /><br />
<br />
<br />
<li>Sept.12 Monday</li><br />
<li>Wang Li: </li><br />
<p>Western Blot</P> <br /><br />
<br />
<li>Sept.13, 2011 Tuesday</li><br />
<li>Sun Weiwen: </li><br />
<p>1. Continue Zhao's experiment to pick the single colony of infusion recA-gfp-pUC18-DH5α, pET28a-T7 promoter knock-out-DH5α, and recA-cheZ(no ter)-gfp-pU18-DH5α. When the colony grows up, conduct the colony PCR of infusion recA-gfp-pUC18-DH5α and recA-cheZ(no ter)-gfp-pU18-DH5α, but there are no expected results. Extract the plasmids of pET28a-T7 promoter knock-out-DH5α, and then digest them with SmaI and XbaI, the system is as following:<br />
ddH2O: 15μl<br />
Buffer 4: 3μl<br />
Plasmid: 10μl<br />
SmaI: 1μl<br />
XbaI: 1μl<br />
In the end, there is also no expected result. </P> <br /><br />
<br />
<li>Wang Zilong, Zheng Yi: </li><br />
<p>Double digest recN, trkD (for biobrick assembling) and plasmid pSB1C3 with XbaI and SpeI, and then ligate the genes to the plasmid. (1 is 1Kb marker, 2 is recN, 3 is trkD, 4 is pSB1C3). </P> <br /><br />
<br />
<li>Sept. 15, 2011 Thursday</li><br />
<li>Wang Zilong, Zheng Yi: </li><br />
<p>Double digest recA, recN and plasmid pUC18-EGFP with XbaI and EcoRI-HF, and then ligate the genes to the plasmid. </P> <br /><br />
<br />
<li>Sept.16, 2011 Friday</li><br />
<li>Sun Weiwen: </li><br />
<p>1. Receive the Cs analysis report of last experiment, and the results are as following:<br />
Control: 23.9mg/Kg (sample weight: about 20g)<br />
Experiment: 32.8mg/Kg (sample weight: about 20g). </P> <br /><br />
<p>2. Repeat the experiments from 2011-9-4 to 2011-9-6, but the original Cs concentration is 0.05g CsCl/100ml LB. Induce the experiment group with IPTG at 18℃ overnight. Then keep them still in the 37℃ incubator for 6 hours. Collect the E.coli for analysis. </P> <br /><br />
<br />
<li>Wang Zilong, Zheng Yi: </li><br />
<p>Transform recA-pUC18-EGFP and recN-pUC18-EGFP to DH5α. </P> <br /><br />
<br />
<li>Sept.17, 2011 Saturday</li><br />
<li>Sun Weiwen: </li><br />
<p>1. Find out that nalidixic acid or mitomycin C have the same effect on Promoter recA or recN. </P> <br /><br />
<p>2. Plan to ligate recA with trkD if the ligation of recN and trkD cannot be accomplished. </P> <br /><br />
<p>3. T7 promoter of pET28a has been cut off. </P> <br /><br />
<br />
<li>Wang Zilong, Zheng Yi: </li><br />
<p>Do Clony PCR on recN-pSB1C3, trkD-pSB1C3, recA-pUC18-EGFP and recN-pUC18-EGFP to test the ligation. </P> <br /><br />
<br />
<br />
<li>Sept. 18, 2011 Sunday</li><br />
<li>Wang Zilong, Zheng Yi: </li><br />
<p>1. Transform cheZ-pET-28a to strain whose cheZ gene is knocked out. </P> <br /><br />
<p>2. Collect plasmid recN-pSB1C3, trkD-pSB1C3, recA-pUC18-EGFP and recN-pUC18-EGFP and double digest the first one with EcoRI and SpeI, second one with EcoRI and XbaI, third one and forth one with EcoRI and HindIII, which shows the false-positive result of recN-pSB1C3, trkD-pSB1C3. </P> <br /><br />
<br />
<br />
<li>Wang Li: </li><br />
<p>Western Blot</P> <br /><br />
<br />
<li>Sept. 19, 2011 Monday</li><br />
<li>Wang Zilong, Zheng Yi: </li><br />
<p>Re-ligate recN and trkD to pSB1C3 and then transform them into DH5α. After culture over night, do clony PCR to test whether the ligation is successful, and both are successful. (1 is 1Kb marker, 2 is recN, 3 is trkD, 4 is pSB1C3). </P> <br /><br />
<br />
<br />
<li>Sept.21, 2011 Wednesday</li><br />
<li>Sun Weiwen: </li><br />
<p>1. Receive the Cs analysis report of last experiment, and the results are as following:<br />
Control: 5.58mg/Kg (sample weight: 23.22g).<br />
Experiment: 5.38mg/Kg (sample weight: 21.17g). </P> <br /><br />
<p>2. Repeat the experiments from 2011-9-4 to 2011-9-6, but the original Cs concentration is 0.6g CsCl/100ml LB. Induce the experiment group with IPTG at 25℃ overnight. Then keep them still in the 37℃ incubator for 6 hours. Collect the E.coli for analysis. </P> <br /><br />
<p>3. Receive the Cs analysis report of last experiment, and the results are as following:<br />
Control: 112mg/Kg (sample weight: 19.20g)<br />
Experiment: 77.9mg/Kg (sample weight: 20.94g). </P> <br /><br />
<li>Sept. 25, 2011 Sunday</li><br />
<li>Wang Zilong, Zheng Yi: </li><br />
<p>Use Go Taq® to PCR trkD (for ligation with recN-pUC18-EGFP). </P> <br /><br />
<br />
<br />
<li>Wang Li: </li><br />
<p>Western Blot Succeed</P> <br /><br />
<br />
<li>Sept. 26, 2011 Monday</li><br />
<li>Wang Zilong, Zheng Yi: </li><br />
<p>1. Amplifying cloning of trkD (for ligation with recN-pUC18-EGFP) with PrimeStar®. </P> <br /><br />
<br />
<p>2. Double digest pSB1C3 with EcoRI and PstI. </P> <br /><br />
<p>3. Gradient PCR genes (recA1, recN2, cheZ3, cheZ'4, trkD5, trkD'6, EGFP7) for biobrick assembling with Go Taq® to search proper condition of PCR. </P> <br /><br />
<br />
<p>4. Double digest trkD (for ligation with recN-pUC18-EGFP) with XbaI and HindIII. </P> <br /><br />
<br />
<li>Sept. 27, 2011 Tuesday</li><br />
<li>Wang Zilong, Zheng Yi: </li><br />
<p>1. Amplifying cloning of genes for biobrick assembling with PrimeStar®. </P> <br /><br />
<br />
<br />
<p>2. Double digest genes for biobrick assembling with EcoRI and PstI, and then ligate them with pSB1C3 digested by same restrictive enzymes. (1~7 are pSB1C3, recA, recN, cheZ, cheZ', trkD, EGFP). </P> <br /><br />
<br />
<br />
<br />
<li>Wang Li: </li><br />
<p>Repeat Western Blot</P> <br /><br />
<br />
<li>Sept. 28, 2011 Wednesday</li><br />
<li>Wang Zilong, Zheng Yi: </li><br />
<p>Transform products of ligation into DH5α, and the results shows the failure of the ligation. And then do this ligation again with new enzymes. </P> <br /><br />
<br />
<li>Sept.30, 2011 Friday</li><br />
<li>Sun Weiwen: </li><br />
<p>1. Zhao has constructed the plasmid recA-gfp-pET28a(T7 knock-out) and recA-gfp-pET28a(T7 knock-out), So I cut of the gfp gene from the two kinds of plasmid. </P> <br /><br />
<p>2. Try to ligate trkD-gfp or only trkD into the two kinds of plasmid. Although some E.coli could grow on the culture containing antibiotic, the results of colony PCR are not expected. In the end, all tries to ligate trkD-gfp or only trkD into the plasmids recA-gfp-pET28a (T7 knock-out) and recA-gfp-pET28a (T7 knock-out) have failed. </P> <br /><br />
<br />
<li>Oct. 1, 2011 Saturday</li><br />
<li>Wang Zilong, Zheng Yi: </li><br />
<p>Re-clone all the genes again and digest them again, then ligate them with plasmid again to test whether the ligation is successful. Do transformation. (1~8 are recA, recN, EGFP, pSB1C3, cheZ, cheZ', trkD, trkD', respecitvely). </P> <br /><br />
<br />
<li>Xu Yitian, Zhao Zhilun: </li><br />
<p>Inoculate two strains: recA-GFP-pET28a(T7 knock-out)-BL21 and recN-GFP-pET28a(T7 knock-out)-BL21. </P> <br /><br />
<li>Oct. 2, 2011 Sunday</li><br />
<li>Wang Zilong, Zheng Yi: </li><br />
<p>Double digest genes for biobrick assembling with XbaI and SpeI, and then ligate them with pSB1C3 digested by same restrictive enzymes. Do transformation. (1~8 are EGFP, trkD, pSB1C3, recA, recN, cheZ, trkD', cheZ', respectively). </P> <br /><br />
<br />
<br />
<li>Wang Li: </li><br />
<p>Exploring E.coli movement induced by NAL or UV. </P> <br /><br />
<br />
<li>Xu Yitian, Zhao Zhilun: </li><br />
<p>1. Mix 1.4 ml each inocula with 70ml LB, then place them into the 37°220rpm shaker. Separate every 10 ml inocula into an aseptic conical flask, and induce with a specific concentration of NAL. There are 6 experiment groups and one control group. Place all the conical flasks into shaker at 37°220rpm for 4h. Then add 200ul to every hole of a microplate and exam the fluorescence intensity by the Microplate Reader. </P> <br /><br />
<p>2. Inoculate the same stains as last night. </P> <br /><br />
<br />
<li>Oct.3, 2011 Monday</li><br />
<li>Sun Weiwen: </li><br />
<p>1. Digest recA-gfp-pET28a (T7 knock-out) and gene trkD with XbaI and HindIII respectively, and then to ligate them together. </P> <br /> <br />
<p>2. Electrotransform the ligation product into E.coli DH5α, and smear them on the solid medium containing Kanamycin. After the colony grows up, pick up the single colony to smear on another medium. After 6 hours' culture, the colony grows up, and then conduct the colony PCR to copy the gene trkD. The result indicates that I have successfully construct the recA-trkD-pET28a (T7 knock-out) plasmid. </P> <br /> <br />
<br />
<br />
<li>Xu Yitian: </li><br />
<p>1. Mix 3.4 ml each inocula with 170ml LB, then place them into the 37°220rpm shaker. Separate every 10 ml inocula into an aseptic conical flask, and induce with a specific concentration of NAL. There are 6 experiment groups and one control group. Place all the conical flasks into shaker at 37°220rpm for 4h. Then regulate OD value of the inocula in every conical flask to 2.023 to 2.033. The remaining 100ml incula was separated to 20 centrifuge tubes. 5ml inocula in every tube. Place all the tubes under the UV light. Take out one tube every 10min and mark the time on the tube. Then add 200ul to every hole of a microplate and exam the fluorescence intensity by the Microplate Reader. </P> <br /><br />
<p>2. Inoculate three stains: the same stains as last night and the strain of BL21. </P> <br /><br />
<li>Oct.4, 2011 Tuesday</li><br />
<p>Mix 1.4 ml each inocula with 70ml LB, then place them into the 37°220rpm shaker. Separate every 10 ml inocula into an aseptic conical flask, and induce with a specific <br />
<h2>Our Protocols</h2><br />
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<h2>Pages we think helpful:</h2><br />
<h1><a href="https://static.igem.org/mediawiki/igem.org/5/5f/Protocols_SYSU.pdf">Download our protocols</a></h1><br />
<br />
<h1><a href="https://2011.igem.org/Team:SYSU-China/page_project_modules_construction">see how we construct our modules</a></h1><br />
<h1><a href="https://2011.igem.org/Team:SYSU-China/page_project_modules_verification">see how we verify each module</a></h1><br />
<h1><a href="https://2011.igem.org/Team:SYSU-China/main_page_story">or see our whole idea in Story page</a></h1><br />
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<p>From the 2011 iGEM team SYSU-China (2011)</p><br />
<p>Sun Yat-Sen University, Guangzhou, China</p><br />
<p>Address: 135# Xingang Rd.(W.), Haizhu Guangzhou, P.R.China</p><br />
<p><a href="http://eng.sysu.edu.cn/">visit the Sun Yat-sen university website</a></p><br />
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</html></div>Cambihttp://2011.igem.org/Team:SYSU-China/page_project_functional_constructionTeam:SYSU-China/page project functional construction2011-10-06T02:28:54Z<p>Cambi: </p>
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<p>&nbsp;&nbsp;&nbsp;&nbsp;After the modules verification, we began further tests of our constructed bacteria to verify that the genes can function well.</p><br />
<p>&nbsp;&nbsp;&nbsp;&nbsp;First, we tested the function of our constructed gene cheZ. Because of limited conditions, we tried to replace ion radiation with common UV light, which has the same effect of DNA damage to activate the SOS signal pathway. When exposed to a certain intensity(which couldn't be measured for a lack of apposite apparatus) of UV light, the E.coli transformed with RecA-CheZ-pET28a was observed a faster movement towards it. </p><br />
<p>&nbsp;&nbsp;&nbsp;&nbsp;Then, we used TrkD-pET32a to test the function of trkD, which could be accomplished through detection of the accumulation of Cs+ in E.coli extract. The results indicate that within the inducement of 0.5mM IPTG at 37℃ for 4h, the experimental group has a significantly higher amount of Cs+ absorption than the control group. </p><br />
<p>&nbsp;&nbsp;&nbsp;&nbsp;In terms of the results above, we have proved that cheZ and trkD constructed in the plasmids functions. Taking these two evidence together, it's not hard to predict that the constructed bacteria obtain the ability to 'swim' towards ion radiation and to absorb Cs+. </p><br />
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<h2>Pages we think helpful:</h2><br />
<h1><a href="https://2011.igem.org/Team:SYSU-China/page_project_modules_construction">see how we construct our modules</a></h1><br />
<h1><a href="https://2011.igem.org/Team:SYSU-China/page_project_modules_verification">see how we verify each module</a></h1><br />
<h1><a href="https://2011.igem.org/Team:SYSU-China/main_page_story">or see our whole idea in Story page</a></h1><br />
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<p>From the 2011 iGEM team SYSU-China (2011)</p><br />
<p>Sun Yat-Sen University, Guangzhou, China</p><br />
<p>Address: 135# Xingang Rd.(W.), Haizhu Guangzhou, P.R.China</p><br />
<p><a href="http://eng.sysu.edu.cn/">visit the Sun Yat-sen university website</a></p><br />
<p>Thanks Apycom jQuery Menus and <a href="http://apycom.com/">visit their website</a><br />
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</html></div>Cambihttp://2011.igem.org/Team:SYSU-China/page_project_modules_verificationTeam:SYSU-China/page project modules verification2011-10-06T02:27:31Z<p>Cambi: </p>
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<div id="page_content_baBar_details"><a href="#TabbedPanels2">See Details</a></div><br />
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<h2>Fluorescence </h2><br />
<div class="page_content_textBar_enterTEXT_enter"><br />
<br /><br />
<p>&nbsp;&nbsp;&nbsp;&nbsp;We decided to examine the expression of the two promoters- PrecA and PrecN, and two genes-cheZ and trkD, via the fluorescent intensity of EGFP whose sequence had been ligated downstream after them. </p><br />
<p>&nbsp;&nbsp;&nbsp;&nbsp;We have constructed a plasmid (pUC18) with EGFP, which is ligated to the plasmid in the PstI and HindIII restriction enzyme cutting sites. Then we link PrecA,PrecN,cheZ,trkD gene to EGFP sequence respectively to construct four plasmids, recA-GFP-pUC18,recN-GFP-pUC18,cheZ-GFP-pUC18 and trkD-GFP-pUC18. We used ultraviolet or nalidixic acid to damage E.coli's DNA to trigger the expression of PrecA and PrecN on in order to examine the expression of EGFP. However, there is a lac promoter upstream before the multi-cloning site of pUC18, which allows us to use IPTG induction to examine the expression of cheZ and trkD by watching the GFP expression. In order to ensure the expression of two genes and GFP, we deleted the terminator of the two genes, cheZ and trkD.</p><br />
<p>&nbsp;&nbsp;&nbsp;&nbsp;Using the laser scanning confocal microscope (LSCM), we can see obvious fluorescence of cheZ-GFP-pUC18 and trkD-GFP-pUC18(Figure 1 and Figure 2). The fluorescence of trkD-GFP-pUC18, is located mostly on the membrane of E.coli. The lac promoter, however, will express without induction of IPTG, resulting in the expression of GFP of control group, which is cheZ-GFP-pUC18 and trkD-GFP-pUC18 that are not induced by IPTG. Consequently, we utilized the Flow Cytometer(FCM) to exam the intensity of GFP in control group and experiment group. (We are still working on that)</p><br />
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<li> <a href="https://static.igem.org/mediawiki/2011/3/3b/SYSU_1_chez-GFP-pUC18-control.jpg" title=""><img src="https://static.igem.org/mediawiki/igem.org/8/87/1_chez-GFP-pUC18-control_S.jpg" width="150" height="126" alt="Flower" /></a> </li><br />
<li> <a href="https://static.igem.org/mediawiki/2011/8/83/SYSU_2_chez-GFP-pUC18-experiment.jpg" title=""><img src="https://static.igem.org/mediawiki/igem.org/4/43/2_chez-GFP-pUC18-experiment_S.jpg" width="150" height="126" alt="Tree" /></a> </li><br />
<li> <a href="https://static.igem.org/mediawiki/2011/e/e1/SYSU_3_trkD-GFP-pUC18-control.jpg" title=""><img src="https://static.igem.org/mediawiki/igem.org/d/d1/3_trkD-GFP-pUC18-control_S.jpg" width="150" height="126" alt="" /></a> </li><br />
<li> <a href="https://static.igem.org/mediawiki/2011/d/d8/SYSU_4_trkd-GFP-pUC18-experiment.jpg" title=""><img src="https://static.igem.org/mediawiki/igem.org/8/81/4_trkd-GFP-pUC18-experiment_S.jpg" width="150" height="126" alt="" /></a> </li><br />
<li> <a href="https://static.igem.org/mediawiki/2011/a/a8/SYSU_5_trkD-GFP-pUC18-control1.jpg" title=""><img src="https://static.igem.org/mediawiki/igem.org/3/36/5_trkD-GFP-pUC18-control1_S.jpg" width="150" height="126" alt="" /></a> </li><br />
<li> <a href="https://static.igem.org/mediawiki/2011/0/0f/SYSU_6_trkd-GFP-pUC18-experiment1.jpg" title=""><img src="https://static.igem.org/mediawiki/igem.org/8/84/6_trkd-GFP-pUC18-experiment1_S.jpg" width="150" height="126" alt="" /></a> </li><br />
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<h2>Western Blot test</h2><br />
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<div class="page_content_textBar_enterTEXT_enter"><p>&nbsp;&nbsp;&nbsp;&nbsp;We planned to exame the expression of gene cheZ and trkD on protein level. Within the inducement of 0.1mg/mL Isopropyl β-D-1-thiogalactopyranoside(IPTG) at 18℃ for 15~18h, we extracted the total proteins of the E.coli transformed with CheZ-pET28a or TrkD-pET32a. Western Blot results showed that the quantity of protein CheZ and TrkD significantly increased after the inducement, indicating that cheZ and trkD expressed well. Additionally, sicne a basal expression caused by promoter lac in both plasmids, the control groups showed a small quantity of proteins. </p></div><br />
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<li> <a href="https://static.igem.org/mediawiki/igem.org/9/92/Western_blot_L.png" title=""><img src="https://static.igem.org/mediawiki/igem.org/a/ae/Westerblot_s.png" width="150" height="137" alt="Flower" /></a> </li><br />
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<h2>Cesium absorption test </h2><br />
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<div class="page_content_textBar_enterTEXT_enter"><br />
<p>&nbsp;&nbsp;&nbsp;&nbsp;We use TrkD-pET32a to test the function of trkD. The test could be accomplished through the detection of the amount of Cs+ absorption in the experiment group and control group. </p><br />
<p>&nbsp;&nbsp;&nbsp;&nbsp;The analysis of Cs+ absorption through trkD indicates that under the inducement of IPTG (0.5mM) for about 4h, the trkD constructed in plasmid can express normally, thus the experiment group has a higher amount of absorption than that of the control group. While the reason why the control has also absorbed Cs+ is that gene trkD also exists in E.coli's genome, so the control group can also express a certain amount of trkD to accomplish the absorption of Cs+. As a result, the absorption of the experiment group is 47.8% higher than that of the control group.</p><br />
<p>&nbsp;&nbsp;&nbsp;&nbsp;In terms of the result, we have proved that the trkD constructed in the plasmid can function normally. </p><br />
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<h2>Characterizing Promoters</h2><br />
<br /><br />
<div class="page_content_textBar_enterTEXT_enter"> <P>&nbsp;&nbsp;&nbsp;&nbsp;Because we want to characterize recA and recN promoters, we turned to Microplate Reader, a machine that can measure the overall fluorescent intensity. We set the exciting wave at 488nm and the emission wave at 520nm, which allow us the measure the fluorescence of recA-GFP-pET28a-BL21 and recA-GFP-pET28a-BL21. After induce with NAL and regulate the OD value of each inocula to a same value, we measured the fluorescence intensity of recA-GFP-pET28a-BL21 and recA-GFP-pET28a-BL21.</P><br />
<p>&nbsp;&nbsp;&nbsp;&nbsp;About the two crests of the curve of recA-GFP-pET28a-BL21, we guess that the rec promoter inside the genome of the BL21 itself, beside the one caused by rec promoter in the plasmid, may be the reason of another crest. At first, the recA promoter is triggered by the increasing NAL concentration, which leads to the first crest. As the NAL concentration increases, the DNA of the plasmid was damaged but the rec promoter in the genome has not been triggered yet since the concentration of the NAL has not reached its threshold, which results in the trough between the two crests. Finally, the rec promoter in the genome is triggered by the relatively high concentration of NAL and produces the protein of rec family, which may help repair the DNA of the plasmid, which causes the second crest of the curve.</p><br />
<p>&nbsp;&nbsp;&nbsp;&nbsp;The curve of recN-GFP-pET28a-BL21 seems to support our hypothesis. The curve of recN-GFP-pET28a-BL21 has a platform instead of a trough. Then a crest after that. We guess that the threshold of recN promoter is higher than recA promoter is the reason. The crest caused by the recN promoter in the plasmid overlappes with the trough, which creates a platform as we see.</p><br />
<p>&nbsp;&nbsp;&nbsp;&nbsp;As the why the the rec promoter of the plasmid will be damaged earlier than that in the genome, we guess the different position of the two promoters is the reason.</p><br />
<p>&nbsp;&nbsp;&nbsp;&nbsp;However, the difference between all the data is not convincing enough, which may lead to another explanation: all the tendency of the data is caused by system error, and yet, which we haven't found out. </p><br />
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<li> <a href="https://static.igem.org/mediawiki/igem.org/f/f3/1.jpg" title=""><img src="https://static.igem.org/mediawiki/igem.org/f/f3/1%27.jpg" width="150" height="137" alt="Flower" /></a> </li><br />
<li> <a href="https://static.igem.org/mediawiki/igem.org/1/15/2.jpg" title=""><img src="https://static.igem.org/mediawiki/igem.org/9/95/2%27.jpg" width="150" height="137" alt="Flower" /></a> </li><br />
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<h2>Pages we think helpful:</h2><br />
<h1><a href="https://2011.igem.org/Team:SYSU-China/page_project_modules_construction">see how we construct our modules</a></h1><br />
<h1><a href="https://2011.igem.org/Team:SYSU-China/page_project_modules_verification">see how we verify each module</a></h1><br />
<h1><a href="https://2011.igem.org/Team:SYSU-China/main_page_story">or see our whole idea in Story page</a></h1><br />
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<p>From the 2011 iGEM team SYSU-China (2011)</p><br />
<p>Sun Yat-Sen University, Guangzhou, China</p><br />
<p>Address: 135# Xingang Rd.(W.), Haizhu Guangzhou, P.R.China</p><br />
<p><a href="http://eng.sysu.edu.cn/">visit the Sun Yat-sen university website</a></p><br />
<p>Thanks Apycom jQuery Menus and <a href="http://apycom.com/">visit their website</a><br />
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<div id="page_content_baBar_details"><a href="#page_content_textBar_enterTEXT">See Details</a></div><br />
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<p>&nbsp;&nbsp;&nbsp;&nbsp;We have designed three categories of modules - Expressional Testing Modules, Functionalizing Testing Modules and Final Functional Modules - to test the expression and function of every element and to accomplish the final function. The followings are the details of these modules.</p><br />
<br /><br />
<h2>1. Expression Testing Modules</h2><br />
<br /><br />
<p>&nbsp;&nbsp;&nbsp;&nbsp;In order to test whether the elements we obtain from E.coli genome PCR can express normally, we have constructed the expressional testing modules through genetic engineering methods. But the existing promoter in the pET series plasmids will affect the expression test of promoter RecA and RecN, so we cut off the T7 promoter of the plasmid pET28a. By digesting promoter RecA and RecN with restriction endonuclease EcoRI and XbaI, digesting EGFP gene with PstI and HindIII, and then ligating them into plasmid pET28a, pET32a and pUC18 with T4 ligase, the following testing modules have been successfully constructed:</p><br />
<br /><br />
<li>a) RecA-gfp-pET28a(promoter knock-out) (figure.1)</li><br />
<li>b) RecN-gfp-pET28a(promoter knock-out) (figure.2)</li><br />
<li>c) CheZ(no terminator)-gfp-pUC18 (figure.3)</li><br />
<li>d) TrkD(no terminator)- gfp-pUC18 (figure.4)</li><br />
<li>e) CheZ-pET32a (figure.5)</li><br />
<li>f) TrkD-pET32a (figure.6)</li><br />
<br /><br />
<p>&nbsp;&nbsp;&nbsp;&nbsp;Through the appearance of the green fluorescence with the first four modules, we can know whether the elements RecA, RecN, CheZ and TrkD express normally as we expected. With the Western-blot tests using the modules CheZ-pET32a and TrkD-pET32a, we can directly know the expression state of CheZ and TrkD.</p><br />
<br /><br />
<h2>2. Functionalizing Testing Modules</h2><br />
<br /><br />
<p>&nbsp;&nbsp;&nbsp;&nbsp;For the purpose of realizing the function that CheZ and TrkD can start to express under the induction of nuclear radiation, we expected to ligate RecA with CheZ, and RecN with TrkD. But the ligation of RecN and TrkD was of great difficulty to be accomplished. This may be caused by the facts that RecN is too short and the overlapping zone of RecN and TrkD has far less than 15 base pairs, which is essential for recombinant PCR. However, TrkD expression under the promotion of RecA satisfies our requirements. As a result, we have constructed the following modules to conduct the functionalizing tests:</p><br />
<br /><br />
<li>a) RecA-CheZ-pET28a(promoter knock-out) (figure.7)</li><br />
<br />
<li>b) RecA-TrkD-pET28a(promoter knock-out) (figure.8)</li><br />
<li>c) RecA- CheZ(no terminator)-gfp-pET28a(promoter knock-out) (figure.9)</li><br />
<li>d) RecA- TrkD(no terminator)-gfp-pET28a(promoter knock-out) (figure.10)</li><br />
<br /><br />
<p>&nbsp;&nbsp;&nbsp;&nbsp;In addition to the modules constructed above, we also intend to construct the following module:</p><br />
<br /><br />
<li>e) RecN-TrkD(no terminator)-ag43- pET28a(promoter knock-out)</li><br />
<br /><br />
<p>&nbsp;&nbsp;&nbsp;&nbsp;With the first two modules, we could test the expression of CheZ and TrkD through the directed motion of E.coli and the Cs+ absorption under the induction of radiation. With the next two modules, the expression of CheZ and TrkD under the radiation induction can be reflected directly through the fluorescence of gfp. With the last module, we could test the expression state of ag43 through whether E.coli cells connect with each other.</p><br />
<br /><br />
<h2>3. Final Functional Modules</h2><br />
<br /><br />
<p>&nbsp;&nbsp;&nbsp;&nbsp;Based on the former construction of plasmids, we expect to integrate the module RecA-CheZ and RecA-TrkD-ag43 in the same E.coli to fulfill the final function that the E.coli could swimming to the source of nuclear leakage and absorb the radioactive Cs+ under the induction of radiation. At the same time, the ag43 in the downstream of TrkD starts to express. When the ag43 protein accumulates above the threshold, E.coli cells could cross link each other, and then be collected to clear the radioactive.<br />
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<li> <a href="https://static.igem.org/mediawiki/2011/3/3b/SYSU_Figure.1_recA-GFP-pET28.png" title=""><img src="https://static.igem.org/mediawiki/igem.org/7/73/Figure.1_recA-GFP-pET28_S.png" width="150" height="97" /></a><br />
<br />
<h1>figure.1</h1><br />
<p>&nbsp;&nbsp;&nbsp;&nbsp;RecA-gfp-pET28a(promoter knock-out) <p></li><br />
<li> <a href="https://static.igem.org/mediawiki/2011/9/92/SYSU_Figure.2_recN-GFP-pET28.png" title=""><img src="https://static.igem.org/mediawiki/igem.org/f/f3/Figure.2_recN-GFP-pET28_S.png" width="150" height="97" /></a><br />
<h1>figure.2</h1><br />
<p>&nbsp;&nbsp;&nbsp;&nbsp;RecN-gfp-pET28a(promoter knock-out) </p><br />
</li><br />
<li> <a href="https://static.igem.org/mediawiki/2011/6/66/SYSU_Figure.3_lac-CheZ-GFP-pUC18.png" title=""><img src="https://static.igem.org/mediawiki/igem.org/5/50/Figure.3_lac-CheZ-GFP-pUC18_S.png" width="150" height="76" /></a><br />
<h1>figure.3</h1><br />
<p>&nbsp;&nbsp;&nbsp;&nbsp;CheZ(no terminator)-gfp-pUC18</p></li><br />
<li> <a href="https://static.igem.org/mediawiki/2011/c/ce/SYSU_Figure.4_lac-TrkD-GFP-pUC18.png" title=""><img src="https://static.igem.org/mediawiki/igem.org/9/94/Figure.4_lac-TrkD-GFP-pUC18_S.png" width="150" height="76"/></a><br />
<h1>figure.4</h1><br />
<p>&nbsp;&nbsp;&nbsp;&nbsp;TrkD(no terminator)- gfp-pUC18</p></li><br />
<li> <a href="https://static.igem.org/mediawiki/2011/8/8f/SYSU_Figure.5_T7-Chez-pET32a.png" title=""><img src="https://static.igem.org/mediawiki/igem.org/5/5e/Figure.5_T7-Chez-pET32a_S.png" width="150" height="97" /></a><br />
<h1>figure.5</h1><br />
<p>&nbsp;&nbsp;&nbsp;&nbsp;CheZ-pET32a</p></li><br />
<li> <a href="https://static.igem.org/mediawiki/2011/0/0d/SYSU_Figure.6_T7-TrkD-pET32a.png" title=""><img src="https://static.igem.org/mediawiki/igem.org/f/fe/Figure.6_T7-TrkD-pET32a_S.png" width="150" height="97" /></a><br />
<h1>figure.6</h1><br />
<p>&nbsp;&nbsp;&nbsp;&nbsp;TrkD-pET32a</p></li><br />
<li> <a href="https://static.igem.org/mediawiki/2011/e/e5/SYSU_Figure.7_recA-CheZ.png" title=""><img src="https://static.igem.org/mediawiki/igem.org/5/5f/Figure.7_recA-CheZ_S.png" width="150" height="97"/></a><br />
<h1>figure.7</h1><br />
<p>&nbsp;&nbsp;&nbsp;&nbsp;RecA-CheZ-pET28a(promoter knock-out)</p></li><br />
<li> <a href="https://static.igem.org/mediawiki/2011/1/13/SYSU_Figure.8_recA-TrkD.png" title=""><img src="https://static.igem.org/mediawiki/igem.org/9/99/Figure.8_recA-TrkD.png" width="150" height="97" /></a><br />
<h1>figure.8</h1><br />
<p>&nbsp;&nbsp;&nbsp;&nbsp;RecA-TrkD-pET28a(promoter knock-out)</p></li><br />
<li> <a href="https://static.igem.org/mediawiki/2011/9/90/SYSU_Figure.9_recA-CheZ-GFP-pET28.png" title=""><img src="https://static.igem.org/mediawiki/igem.org/b/b8/Figure.9_recA-CheZ-GFP-pET28_S.png" width="150" height="76" /></a><br />
<h1>figure.9</h1><br />
<p>&nbsp;&nbsp;&nbsp;&nbsp;RecA- CheZ(no terminator)-gfp-pET28a(promoter knock-out)</p></li><br />
<li> <a href="https://static.igem.org/mediawiki/2011/9/90/SYSU_Figure.10_recA-trkd-GFP-pET28.png" title=""><img src="https://static.igem.org/mediawiki/igem.org/e/e0/Figure.10_recA-trkd-GFP-pET28.png" width="150" height="76" /></a><br />
<h1>figure.10</h1><br />
<p>&nbsp;&nbsp;&nbsp;&nbsp;RecA- TrkD(no terminator)-gfp-pET28a(promoter knock-out)</p></li><br />
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<h2>Pages we think helpful:</h2><br />
<h1><a href="https://2011.igem.org/Team:SYSU-China/page_project_modules_verification">see how we verify each module</a></h1><br />
<h1><a href="https://2011.igem.org/Team:SYSU-China/main_page_story">or see our whole idea in Story page</a></h1><br />
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<p>From the 2011 iGEM team SYSU-China (2011)</p><br />
<p>Sun Yat-Sen University, Guangzhou, China</p><br />
<p>Address: 135# Xingang Rd.(W.), Haizhu Guangzhou, P.R.China</p><br />
<p><a href="http://eng.sysu.edu.cn/">visit the Sun Yat-sen university website</a></p><br />
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</html></div>Cambihttp://2011.igem.org/Team:SYSU-China/page_human_practice_surveyTeam:SYSU-China/page human practice survey2011-10-06T02:25:13Z<p>Cambi: </p>
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<li><a href="https://2011.igem.org/Team:SYSU-China/page_project_modules_construction"><span>Modules Construction</span></a><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/page_project_modules_verification"><span>Modules Verification</span></a></li><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/page_project_functional_construction"><span>Functional Verification</span></a></li><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/page_project_notes"><span>Notes</span></a></li><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/page_project_datapage"><span>Date Page</span></a></li><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/page_project_safety"><span>Safety</span></a></li><br />
</ul><br />
</div><br />
</li><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/main_page_human_practice" class="parent"><span>HUMAN PRACTICE</span></a><br />
<div><br />
<ul><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/page_human_practice_app"><span>App</span></a><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/page_human_practice_labcraft"><span>LabCraft Board Game</span></a></li><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/page_human_practice_workshop"><span>Workshop</span></a></li><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/page_human_practice_survey"><span>Survey</span></a></li><br />
</ul><br />
</div><br />
</li><br />
<br />
<li><a href="https://2011.igem.org/Team:SYSU-China/main_page_aboutus" class="parent"><span>ABOUT US</span></a><br />
<div><br />
<ul><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/page_aboutus_team_members"><span>Team Members</span></a><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/page_aboutus_special_thanks"><span>Spcial Thanks</span></a></li><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/page_aboutus_LT"><span>Logo and T-shirt</span></a></li><br />
</ul><br />
</div><br />
</li><br />
<br />
</ul><br />
</div><!--here ends the manu!--><br />
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<div class="page_clear_and_introBar"><br />
<img src="https://static.igem.org/mediawiki/igem.org/b/ba/Page_human_pratice_survey_introBar.png" width="958" height="65" /></div><br />
<!------here begins the main content section--!--><br />
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<img src="https://static.igem.org/mediawiki/igem.org/b/b6/Page_human_pratice_survey_bg.jpg" width="632" height="600" /><br />
<div id="page_content_baBar_details"><a href="#page_content_textBar_enterTEXT">See Details</a></div><br />
</div><br />
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<div id="page_content_textBar_enterTEXT"><br />
<!----------text section begins----------------!--> <br />
<br />
<br />
<p>&nbsp;&nbsp;&nbsp;&nbsp;We have conducted a survey to learn about what people think about the synthetic biology. To get more information, we tried to do the survey among people with different background. Most people know something about synthetic biology more or less, and they show some interest in synthetic biology and its applications. The result is shown below. </p><br />
<p>&nbsp;</p><br />
<img src="https://static.igem.org/mediawiki/2011/3/34/Survey1_s.png" width="406" height="537" /><br />
<p>&nbsp;&nbsp;&nbsp;&nbsp;From the two pie charts above, we can see that most people have some knowledge about the synthetic biology and have interests in it. As the words "Synthia" and synthetic biology appear more in the public media, it becomes a hot topic, especially on the issue of bioethics, so we set a question about that.</p><br />
<p>&nbsp;</p><br />
<img src="https://static.igem.org/mediawiki/2011/4/44/Survey_pic2.png" width="406" height="301" /><br />
<p>&nbsp;&nbsp;&nbsp;&nbsp;Most people show some worries about the biosafety, but still think synbio is a promising technique. So we should pay more attention to safety and ethics, while doing experiments.</p><br />
<p>&nbsp;</p><br />
<img src="https://static.igem.org/mediawiki/2011/b/b6/Survey_pic3.png" width="406" height="525" /><br />
<p>&nbsp;&nbsp;&nbsp;&nbsp;As we all know, interdisciplinary study get more attention recently, and synthetic biology is typical in this term. As the result shows, more people think interdisciplinary study will become more important, and more and more people have the idea that other disciplines will contribute to the synthetic biology.</p><br />
<p>&nbsp;</p><br />
<img src="https://static.igem.org/mediawiki/2011/e/eb/Survey_pic4.png" width="406" height="258" /><br />
<p>&nbsp;&nbsp;&nbsp;&nbsp;In terms of the ultimate object of synthetic biology, most people give their vote to the application, and in our opinion, the application is also the ultimate object of iGEM.</p><br />
<br />
<br />
<!-----------text section ends--------------!--> <br />
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<div class="main_page_clearer_light"></div><br />
</div> <br />
</div><br />
</div><br />
<!---------------------------------------------!--><br />
<div class="page_content_RsideBar"><br />
<div class="page_content_videoBar"><br />
<h1>Please watch our animination</h1><br />
<iframe width="307" height="225" src="http://www.youtube.com/embed/KymdcolAhcE?rel=0" frameborder="0" allowfullscreen></iframe><br />
<br />
<br/><br />
<br />
</div><br />
<div class="page_content_relatedBar_humanpractice_survey"><br />
<h2>Pages we think helpful:</h2><br />
<h1><a href="https://2011.igem.org/Team:SYSU-China/page_human_practice_labcraft">check out LabCraft board game</a></h1><br />
<h1><a href="http://biogeek.kilu.de/Lab_Craft_Guide_Book.pdf">Download our LabCraft Guide Book</a></h1><br />
<br />
<h1><a href="http://itunes.apple.com/us/app/synbio-igem-intro/id468666589?ls=1&mt=8">iTune page for SynBio Intro</a></h1><br />
<h1><a href="https://2011.igem.org/Team:SYSU-China/main_page_human_practice">our human practice main page</a></h1><br />
<h1><a href="https://2011.igem.org/Team:SYSU-China/main_page_story">or our Story page</a></h1><br />
</div><br />
</div> <br />
<!---------------------------------------------!--><br />
<div class="main_page_clearer_light"></div><br />
<!---------------------------------------------!--> <br />
<br />
</div><!--here ends the page_content_WholeBox!--><br />
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</div><!--here ends the mainpage_project_WholeContainer!--><br />
<br />
<!--here begins the contact_bottombar!--><br />
<div class="contact_bottombar"><br />
<p>From the 2011 iGEM team SYSU-China (2011)</p><br />
<p>Sun Yat-Sen University, Guangzhou, China</p><br />
<p>Address: 135# Xingang Rd.(W.), Haizhu Guangzhou, P.R.China</p><br />
<p><a href="http://eng.sysu.edu.cn/">visit the Sun Yat-sen university website</a></p><br />
<p>Thanks Apycom jQuery Menus and <a href="http://apycom.com/">visit their website</a><br />
</div><br />
<!--here ends the contact_bottombar!--><br />
<br />
</div><!--here ends the backgroud_shadow_bar!--><br />
</body><br />
</html></div>Cambihttp://2011.igem.org/Team:SYSU-China/page_human_practice_workshopTeam:SYSU-China/page human practice workshop2011-10-06T02:23:50Z<p>Cambi: </p>
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<ul class="menu"><br />
<li><a href="index.thml"><span></span></a></li><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/main_page_news"><span>NEWS</span></a></li><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/main_page_story"><span>STORY</span></a></li> <br />
<li><a href="https://2011.igem.org/Team:SYSU-China/main_page_project" class="parent"><span>PROJECT</span></a><br />
<div><br />
<ul><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/page_project_modules_construction"><span>Modules Construction</span></a><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/page_project_modules_verification"><span>Modules Verification</span></a></li><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/page_project_functional_construction"><span>Functional Verification</span></a></li><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/page_project_notes"><span>Notes</span></a></li><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/page_project_datapage"><span>Date Page</span></a></li><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/page_project_safety"><span>Safety</span></a></li><br />
</ul><br />
</div><br />
</li><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/main_page_human_practice" class="parent"><span>HUMAN PRACTICE</span></a><br />
<div><br />
<ul><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/page_human_practice_app"><span>App</span></a><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/page_human_practice_labcraft"><span>LabCraft Board Game</span></a></li><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/page_human_practice_workshop"><span>Workshop</span></a></li><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/page_human_practice_survey"><span>Survey</span></a></li><br />
</ul><br />
</div><br />
</li><br />
<br />
<li><a href="https://2011.igem.org/Team:SYSU-China/main_page_aboutus" class="parent"><span>ABOUT US</span></a><br />
<div><br />
<ul><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/page_aboutus_team_members"><span>Team Members</span></a><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/page_aboutus_special_thanks"><span>Spcial Thanks</span></a></li><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/page_aboutus_LT"><span>Logo and T-shirt</span></a></li><br />
</ul><br />
</div><br />
</li><br />
<br />
</ul><br />
</div><!--here ends the manu!--><br />
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<!--------here ends the title_section------!--><br />
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<div id="page_content_baBar_details"><a href="#page_content_textBar_enterTEXT">See Details</a></div><br />
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<p>&nbsp;&nbsp;&nbsp;&nbsp;We ran a workshop in our school with junior students. For more effective group discussion, we limit the participants to no more than 15 students. </p><br />
<p>&nbsp;&nbsp;&nbsp;&nbsp;Firstly, we introduced the concept and achievements of synthetic biology. Then we talked about the Biobrick and iGEM. As we think, the workshop is not only for iGEM or synthetic biology, we also spend much time talking about problems they maybe meet in their future study.</p><br />
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<li> <a href="https://static.igem.org/mediawiki/igem.org/2/21/NEO_IMG_IMG_4418.jpg" title=""><img src="https://static.igem.org/mediawiki/igem.org/7/76/NEO_IMG_IMG_4418s.jpg" width="150" height="92" alt="Flower" /></a> </li><br />
<li> <a href="https://static.igem.org/mediawiki/igem.org/3/38/NEO_IMG_IMG_4420.jpg" title=""><img src="https://static.igem.org/mediawiki/igem.org/f/fb/NEO_IMG_IMG_4420s.jpg" width="150" height="100" alt="Tree" /></a> </li><br />
<li> <a href="https://static.igem.org/mediawiki/igem.org/d/d7/NEO_IMG_IMG_4426.jpg" title=""><img src="https://static.igem.org/mediawiki/igem.org/b/b5/NEO_IMG_IMG_4426s.jpg" width="150" height="100" alt="" /></a> </li><br />
<li> <a href="https://static.igem.org/mediawiki/igem.org/4/4f/NEO_IMG_IMG_4430.jpg" title=""><img src="https://static.igem.org/mediawiki/igem.org/8/8c/NEO_IMG_IMG_4430s.jpg" width="150" height="75" alt="" /></a> </li><br />
<li> <a href="https://static.igem.org/mediawiki/igem.org/3/30/NEO_IMG_IMG_4439.jpg" title=""><img src="https://static.igem.org/mediawiki/igem.org/5/50/NEO_IMG_IMG_4439s.jpg" width="150" height="113" alt="" /></a> </li><br />
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</div><br />
</div><br />
<!---------------------------------------------!--><br />
<div class="page_content_RsideBar"><br />
<div class="page_content_videoBar"><br />
<h1>Please watch our animination</h1><br />
<iframe width="307" height="225" src="http://www.youtube.com/embed/KymdcolAhcE?rel=0" frameborder="0" allowfullscreen></iframe><br />
<br />
<br/> <br />
<br />
</div><br />
<div class="page_content_relatedBar_humanpractice_workshop"><br />
<h2>Pages we think helpful:</h2><br />
<h1><a href="https://2011.igem.org/Team:SYSU-China/page_human_practice_labcraft">check out LabCraft board game</a></h1><br />
<h1><a href="http://biogeek.kilu.de/Lab_Craft_Guide_Book.pdf">Download our LabCraft Guide Book</a></h1><br />
<br />
<h1><a href="http://itunes.apple.com/us/app/synbio-igem-intro/id468666589?ls=1&mt=8">iTune page for SynBio Intro</a></h1><br />
<h1><a href="https://2011.igem.org/Team:SYSU-China/main_page_human_practice">our human practice main page</a></h1><br />
<h1><a href="https://2011.igem.org/Team:SYSU-China/main_page_story">or our Story page</a></h1><br />
</div><br />
</div> <br />
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<!--here begins the contact_bottombar!--><br />
<div class="contact_bottombar"><br />
<p>From the 2011 iGEM team SYSU-China (2011)</p><br />
<p>Sun Yat-Sen University, Guangzhou, China</p><br />
<p>Address: 135# Xingang Rd.(W.), Haizhu Guangzhou, P.R.China</p><br />
<p><a href="http://eng.sysu.edu.cn/">visit the Sun Yat-sen university website</a></p><br />
<p>Thanks Apycom jQuery Menus and <a href="http://apycom.com/">visit their website</a><br />
</div><br />
<!--here ends the contact_bottombar!--><br />
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</html></div>Cambihttp://2011.igem.org/Team:SYSU-China/page_human_practice_labcraftTeam:SYSU-China/page human practice labcraft2011-10-06T02:23:12Z<p>Cambi: </p>
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$(function(){<br />
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//<br />
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$("#page_content_baBar_details").scrollTo(700)<br />
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<!--------here begins the manu bar section--!--><br />
<div class="title_container"><br />
<div id="menu"><br />
<ul class="menu"><br />
<li><a href="index.thml"><span></span></a></li><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/main_page_news"><span>NEWS</span></a></li><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/main_page_story"><span>STORY</span></a></li> <br />
<li><a href="https://2011.igem.org/Team:SYSU-China/main_page_project" class="parent"><span>PROJECT</span></a><br />
<div><br />
<ul><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/page_project_modules_construction"><span>Modules Construction</span></a><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/page_project_modules_verification"><span>Modules Verification</span></a></li><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/page_project_functional_construction"><span>Functional Verification</span></a></li><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/page_project_notes"><span>Notes</span></a></li><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/page_project_datapage"><span>Date Page</span></a></li><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/page_project_safety"><span>Safety</span></a></li><br />
</ul><br />
</div><br />
</li><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/main_page_human_practice" class="parent"><span>HUMAN PRACTICE</span></a><br />
<div><br />
<ul><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/page_human_practice_app"><span>App</span></a><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/page_human_practice_labcraft"><span>LabCraft Board Game</span></a></li><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/page_human_practice_workshop"><span>Workshop</span></a></li><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/page_human_practice_survey"><span>Survey</span></a></li><br />
</ul><br />
</div><br />
</li><br />
<br />
<li><a href="https://2011.igem.org/Team:SYSU-China/main_page_aboutus" class="parent"><span>ABOUT US</span></a><br />
<div><br />
<ul><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/page_aboutus_team_members"><span>Team Members</span></a><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/page_aboutus_special_thanks"><span>Spcial Thanks</span></a></li><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/page_aboutus_LT"><span>Logo and T-shirt</span></a></li><br />
</ul><br />
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<div class="page_clear_and_introBar"><br />
<img src="<br />
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<div class="page_content_LsideBar"><br />
<div class="page_content_bgBar"><img src="https://static.igem.org/mediawiki/igem.org/2/2d/Page_human_pratice_lab_craft_bg.jpg" width="632" height="600" /><br />
<div id="page_content_baBar_details"><a href="#page_content_textBar_enterTEXT">See Details</a></div><br />
</div><br />
<div class="page_content_textBar"><br />
<div id="page_content_textBar_enterTEXT"><br />
<div class="page_content_textBar_enterTEXT_enter"><br />
<h2>Click <a href="http://biogeek.kilu.de/Lab_Craft_Guide_Book.pdf">HERE</a> to download LabCraft Guide Book</h2><br />
<br /><br />
<h2>Welcome to the World of LabCraft</h2><br />
<br /><br />
<p>&nbsp;&nbsp;&nbsp;&nbsp;LabCraft is a shootout game that happens in a biological lab. In the lab, Everything is like haveing a war.</p><br />
<br />
<p>&nbsp;&nbsp;&nbsp;&nbsp;The game itself focus between a group of Postdocs and the PI, who is their primary target. The PhDs incognito help the PI, but there is also a Master pursuing his own goal! </p><br />
<br />
<p>&nbsp;&nbsp;&nbsp;&nbsp;In LabCraft each player plays one of these roles, and represents a famous lab character.</p> <br />
<br />
<p>&nbsp;&nbsp;&nbsp;&nbsp;Have fun and enjoy yourself with your friends, classmates, colleagues, even your teachers.<br />
<br /><br />
<br /><br />
<h2>CONTENTS</h2><br />
<br /><br />
<p>&nbsp;&nbsp;&nbsp;&nbsp;119 cards of three different types, they can be identified by their back:</p><br />
<br />
<h1>7 "ROLE" cards:</h1> <br />
<p>&nbsp;&nbsp;&nbsp;&nbsp;1 PI, 2 PhDs, 3 Postdocs, 1 Master.</p><br />
<br />
<h1>8 "CHARACTER" cards,</h1> <br />
<p>&nbsp;&nbsp;&nbsp;&nbsp;with colonies printed on the left side;.</p><br />
<br />
<h1>104 playing cards,</h1><br />
<p>&nbsp;&nbsp;&nbsp;&nbsp;also called funtional cards, printed "LabCraft" on their back.</p><br />
<br />
<h1>"colonies" cards</h1><br />
<p>&nbsp;&nbsp;&nbsp;&nbsp;And by the way, in the box we also offered several "colonies" cards printed with three, four or five colonies (circles that represented the blood/life you left).</p><br />
<br /><br />
<br /><br />
<h2>OBJECT</h2><br />
<br /><br />
<p>&nbsp;&nbsp;&nbsp;&nbsp;Each player has his own goal:</p><br />
<br />
<h1>PI</h1><br />
<p>&nbsp;&nbsp;&nbsp;&nbsp;They must eliminate all the Postdocs and the Master, to protect the lab.</p><br />
<br />
<h1>Postdoc</h1><br />
<p>&nbsp;&nbsp;&nbsp;&nbsp;They would like to eliminate the PI, but they have no scruples about eliminating each other to gain rewards!</p><br />
<br />
<h1>PhD</h1><br />
<p>&nbsp;&nbsp;&nbsp;&nbsp;They help and protect the PI, and share his same goal, at all costs!</p><br />
<br />
<h1>Master</h1><br />
<p>&nbsp;&nbsp;&nbsp;&nbsp;He wants to be the new PI; his goal is to be the last character in play.</p><br />
<br /><br />
<br /><br />
<h2>CHARACTERS</h2><br />
<br /><br />
<p>&nbsp;&nbsp;&nbsp;&nbsp;Each lab character has some special abilities, which make him unique. The series of colonies near the character's picture show how many life points the character begins with, i.e. how many times he can be hit before being eliminated from play. </p><br />
<br />
<p>Moreover, the conlonies indicate how many cards the player can hold in his hand at the end of his turn (hand size limit).</p><br />
<br /><br />
<br /><br />
<h2>THE GAME</h2><br />
<br /><br />
<p>&nbsp;&nbsp;&nbsp;&nbsp;The game is played in turns, in clockwise order, with a beginning from PI. Each player's turn is divided into three phases:</p><br />
<br />
<h1>1. Draw two cards;</h1><br />
<h1>2. Play any number of cards;</h1><br />
<h1>3. Discard excess cards.</h1><br />
<br />
<br />
<li>&nbsp;&nbsp;&nbsp;&nbsp;1. Draw two cards</li><br />
<p>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;The active player draws the top two cards from the draw pile. As soon as the draw pile is empty, shuffle the discard pile to create a new playing deck.</p><br />
<br />
<li>&nbsp;&nbsp;&nbsp;&nbsp;2. Play any number of cards</li><br />
<p>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Now the player may play cards to help himself or hurt the other players, trying to eliminate them. He is not forced to play cards during this phase. Any number of cards may be played.</p><br />
<br />
<p>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;There are only two limitations:</p><br />
<li>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;1) Only one Antibiotic! card may be played per turn;</li><br />
<li>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;2) No player can ever have two identical cards face up in front of him.</li><br />
<br />
<p>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;When a card is played, just follow the instruction on it. Cards can be played only during your turn (with the exception of "Medium" and "Biofilm!" cards).</p><br />
<br />
<p>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Normally a card has an effect which is immediately resolved, and then the card is discarded. However, "Equipments" cards have long-lasting effects, and are kept on the table face up in front of you. The effect of these cards ("in play" cards) lasts until they are discarded or removed somehow, or a special event occurs .</p><br />
<br />
<li>&nbsp;&nbsp;&nbsp;&nbsp;3. Discard excess cards</li><br />
<p>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Once the second phase is over (you do not want to or cannot play any more cards), then you must discard from your hand any cards exceeding your hand-size limit. Remember that the hand size limit of a player, at the end of his turn, is equal to the number of life points he left. </p><br />
<br />
<p>Then it is the next player's turn, in clockwise order.</p><br />
<br /><br /><br />
<p>&nbsp;&nbsp;&nbsp;&nbsp;Please download the Guide Book of LabCraft (PDF) to learn more about our LabCraft.</p><br />
<br />
</div><br />
<br />
<div class="page_content_text_Bar_enterTEXT_RpicBar"><br />
<div id="gallery" class="lbGallery"><br />
<ul><br />
<li> <a href="https://static.igem.org/mediawiki/igem.org/5/5d/Figure_1_update.jpg" title=""><img src="https://static.igem.org/mediawiki/igem.org/9/91/Figure_1_s.jpg" /></a> </li><br />
<h2>Figure 1.</h2>The box art (front cover) of the board game.<br />
<li> <a href="https://static.igem.org/mediawiki/igem.org/8/80/Figure_2.jpg" title=""><img src="https://static.igem.org/mediawiki/igem.org/0/09/Figure_2_s.jpg" /></a> </li><br />
<h2>Figure 2.</h2>The "Character" cards.<br />
<li> <a href="https://static.igem.org/mediawiki/igem.org/c/c9/Figure_3.jpg" title=""><img src="https://static.igem.org/mediawiki/igem.org/2/29/Figure_3_s.jpg" /></a> </li><br />
<h2>Figure 3.</h2>The three basic game playing (or functional) cards.<br />
</ul><br />
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</div><br />
</div><br />
<!---------------------------------------------!--><br />
<div class="page_content_RsideBar"><br />
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<h1>Please watch our animination</h1><br />
<iframe width="307" height="225" src="http://www.youtube.com/embed/KymdcolAhcE?rel=0" frameborder="0" allowfullscreen></iframe><br />
<br />
<br/><br />
<br />
</div><br />
<div class="page_content_relatedBar_humanpractice_LB"><br />
<h2>Pages we think helpful:</h2><br />
<h1><a href="http://biogeek.kilu.de/Lab_Craft_Guide_Book.pdf">Download our LabCraft Guide Book</a></h1><br />
<br />
<h1><a href="http://itunes.apple.com/us/app/synbio-igem-intro/id468666589?ls=1&mt=8">iTune page for SynBio Intro</a></h1><br />
<h1><a href="https://2011.igem.org/Team:SYSU-China/main_page_human_practice">our human practice main page</a></h1><br />
<h1><a href="https://2011.igem.org/Team:SYSU-China/main_page_story">or our Story page</a></h1><br />
<p>&nbsp;</p><br />
</div><br />
</div> <br />
<!---------------------------------------------!--><br />
<div class="main_page_clearer_light"></div><br />
<!---------------------------------------------!--> <br />
<br />
</div><!--here ends the page_content_WholeBox!--><br />
<div class="main_page_clearer_light"></div><br />
</div><!--here ends the mainpage_project_WholeContainer!--><br />
<br />
<!--here begins the contact_bottombar!--><br />
<div class="contact_bottombar"><br />
<p>From the 2011 iGEM team SYSU-China (2011)</p><br />
<p>Sun Yat-Sen University, Guangzhou, China</p><br />
<p>Address: 135# Xingang Rd.(W.), Haizhu Guangzhou, P.R.China</p><br />
<p><a href="http://eng.sysu.edu.cn/">visit the Sun Yat-sen university website</a></p><br />
<p>Thanks Apycom jQuery Menus and <a href="http://apycom.com/">visit their website</a><br />
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</html></div>Cambihttp://2011.igem.org/Team:SYSU-China/page_human_practice_appTeam:SYSU-China/page human practice app2011-10-06T02:19:52Z<p>Cambi: </p>
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<li><a href="index.thml"><span></span></a></li><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/main_page_news"><span>NEWS</span></a></li><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/main_page_story"><span>STORY</span></a></li> <br />
<li><a href="https://2011.igem.org/Team:SYSU-China/main_page_project" class="parent"><span>PROJECT</span></a><br />
<div><br />
<ul><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/page_project_modules_construction"><span>Modules Construction</span></a><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/page_project_modules_verification"><span>Modules Verification</span></a></li><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/page_project_functional_construction"><span>Functional Verification</span></a></li><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/page_project_notes"><span>Notes</span></a></li><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/page_project_datapage"><span>Date Page</span></a></li><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/page_project_safety"><span>Safety</span></a></li><br />
</ul><br />
</div><br />
</li><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/main_page_human_practice" class="parent"><span>HUMAN PRACTICE</span></a><br />
<div><br />
<ul><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/page_human_practice_app"><span>App</span></a><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/page_human_practice_labcraft"><span>LabCraft Board Game</span></a></li><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/page_human_practice_workshop"><span>Workshop</span></a></li><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/page_human_practice_survey"><span>Survey</span></a></li><br />
</ul><br />
</div><br />
</li><br />
<br />
<li><a href="https://2011.igem.org/Team:SYSU-China/main_page_aboutus" class="parent"><span>ABOUT US</span></a><br />
<div><br />
<ul><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/page_aboutus_team_members"><span>Team Members</span></a><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/page_aboutus_special_thanks"><span>Spcial Thanks</span></a></li><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/page_aboutus_LT"><span>Logo and T-shirt</span></a></li><br />
</ul><br />
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<br />
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<!--------here ends the title_section------!--><br />
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<div class="page_clear_and_introBar"><br />
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<!------here begins the main content section--!--><br />
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<div id="page_content_baBar_details"><a href="#page_content_textBar_enterTEXT">See Details</a></div><br />
</div><br />
<div class="page_content_textBar"><br />
<div id="page_content_textBar_enterTEXT"><br />
<h2>SynBio Intro</h2><br />
<br /><br />
<p>&nbsp;&nbsp;&nbsp;&nbsp;SynBio Intro is an app runs on iPhone / iPod Touch. It was designed to provide an all-in-one experience of modern biotechnology from explaining &ldquo;what is DNA&rdquo; to building an organism on your own. With easy language and friendly UI, you will get a joyful but deep impression on how synthetic biology works and change our lives.</p><br />
<p>&nbsp;&nbsp;&nbsp;&nbsp;The app is under review and will be available soon on <a href="http://itunes.apple.com/us/app/synbio-igem-intro/id468666589?ls=1&amp;mt=8">http://itunes.apple.com/us/app/synbio-igem-intro/id468666589?ls=1&amp;mt=8</a></p><br />
</div> <br />
</div><br />
</div><br />
<!---------------------------------------------!--><br />
<div class="page_content_RsideBar"><br />
<div class="page_content_videoBar"><br />
<h1>Please watch our animination</h1><br />
<iframe width="307" height="225" src="http://www.youtube.com/embed/KymdcolAhcE?rel=0" frameborder="0" allowfullscreen></iframe><br />
<br />
<br/><br />
<br />
</div><br />
<div class="page_content_relatedBar_humanpractice_app"><br />
<h2>Pages we think helpful:</h2><br />
<h1><a href="http://itunes.apple.com/us/app/synbio-igem-intro/id468666589?ls=1&mt=8">iTune page for SynBio Intro</a></h1><br />
<h1><a href="https://2011.igem.org/Team:SYSU-China/main_page_human_practice">our human practice main page</a></h1><br />
<h1><a href="https://2011.igem.org/Team:SYSU-China/main_page_story">or our Story page</a></h1><br />
<p>&nbsp;</p><br />
</div><br />
</div> <br />
<!---------------------------------------------!--><br />
<div class="main_page_clearer_light"></div><br />
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<br />
</div><!--here ends the page_content_WholeBox!--><br />
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</div><!--here ends the mainpage_project_WholeContainer!--><br />
<br />
<!--here begins the contact_bottombar!--><br />
<div class="contact_bottombar"><br />
<p>From the 2011 iGEM team SYSU-China (2011)</p><br />
<p>Sun Yat-Sen University, Guangzhou, China</p><br />
<p>Address: 135# Xingang Rd.(W.), Haizhu Guangzhou, P.R.China</p><br />
<p><a href="http://eng.sysu.edu.cn/">visit the Sun Yat-sen university website</a></p><br />
<p>Thanks Apycom jQuery Menus and <a href="http://apycom.com/">visit their website</a><br />
</div><br />
<!--here ends the contact_bottombar!--><br />
<br />
</div><!--here ends the backgroud_shadow_bar!--><br />
</body><br />
</html></div>Cambihttp://2011.igem.org/Team:SYSU-China/page_human_practice_appTeam:SYSU-China/page human practice app2011-10-06T02:18:55Z<p>Cambi: </p>
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// $("#page_content_baBar_details").scrollTo(600,2横向)<br />
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<li><a href="https://2011.igem.org/Team:SYSU-China/page_project_modules_construction"><span>Modules Construction</span></a><br />
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<li><a href="https://2011.igem.org/Team:SYSU-China/page_project_safety"><span>Safety</span></a></li><br />
</ul><br />
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</li><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/main_page_human_practice" class="parent"><span>HUMAN PRACTICE</span></a><br />
<div><br />
<ul><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/page_human_practice_app"><span>App</span></a><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/page_human_practice_labcraft"><span>LabCraft Board Game</span></a></li><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/page_human_practice_workshop"><span>Workshop</span></a></li><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/page_human_practice_survey"><span>Survey</span></a></li><br />
</ul><br />
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</li><br />
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<li><a href="https://2011.igem.org/Team:SYSU-China/page_aboutus_team_members"><span>Team Members</span></a><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/page_aboutus_special_thanks"><span>Spcial Thanks</span></a></li><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/page_aboutus_LT"><span>Logo and T-shirt</span></a></li><br />
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<div class="page_content_textBar"><br />
<div id="page_content_textBar_enterTEXT"><br />
<h2>SynBio Intro</h2><br />
<br /><br />
<p>&nbsp;&nbsp;&nbsp;&nbsp;SynBio Intro is an app runs on iPhone / iPod Touch. It was designed to provide an all-in-one experience of modern biotechnology from explaining &ldquo;what is DNA&rdquo; to building an organism on your own. With easy language and friendly UI, you will get a joyful but deep impression on how synthetic biology works and change our lives.</p><br />
<p>&nbsp;&nbsp;&nbsp;&nbsp;The app is under review and will be available soon on <a href="http://itunes.apple.com/us/app/synbio-igem-intro/id468666589?ls=1&amp;mt=8">http://itunes.apple.com/us/app/synbio-igem-intro/id468666589?ls=1&amp;mt=8</a></p><br />
</div> <br />
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<br />
<br/><br />
<br />
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<h2>Pages we think helpful:</h2><br />
<h1><a href="http://itunes.apple.com/us/app/synbio-igem-intro/id468666589?ls=1&mt=8">iTune page for SynBio Intro</a></h1><br />
<h1><a href="https://2011.igem.org/Team:SYSU-China/main_page_human_practice">our human practice main page</a></h1><br />
<h1><a href="https://2011.igem.org/Team:SYSU-China/main_page_story">or our Story page</a></h1><br />
<p>&nbsp;</p><br />
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<p>From the 2011 iGEM team SYSU-China (2011)</p><br />
<p>Sun Yat-Sen University, Guangzhou, China</p><br />
<p>Address: 135# Xingang Rd.(W.), Haizhu Guangzhou, P.R.China</p><br />
<p><a href="http://eng.sysu.edu.cn/">visit the Sun Yat-sen university website</a></p><br />
<p>Thanks Apycom jQuery Menus and <a href="http://apycom.com/">visit their website</a><br />
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<li><a href="https://2011.igem.org/Team:SYSU-China/main_page_story"><span>STORY</span></a></li> <br />
<li><a href="https://2011.igem.org/Team:SYSU-China/main_page_project" class="parent"><span>PROJECT</span></a><br />
<div><br />
<ul><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/page_project_modules_construction"><span>Modules Construction</span></a><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/page_project_modules_verification"><span>Modules Verification</span></a></li><br />
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<li><a href="https://2011.igem.org/Team:SYSU-China/page_project_notes"><span>Notes</span></a></li><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/page_project_datapage"><span>Date Page</span></a></li><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/page_project_safety"><span>Safety</span></a></li><br />
</ul><br />
</div><br />
</li><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/main_page_human_practice" class="parent"><span>HUMAN PRACTICE</span></a><br />
<div><br />
<ul><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/page_human_practice_app"><span>App</span></a><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/page_human_practice_labcraft"><span>LabCraft Board Game</span></a></li><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/page_human_practice_workshop"><span>Workshop</span></a></li><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/page_human_practice_survey"><span>Survey</span></a></li><br />
</ul><br />
</div><br />
</li><br />
<br />
<li><a href="https://2011.igem.org/Team:SYSU-China/main_page_aboutus" class="parent"><span>ABOUT US</span></a><br />
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<ul><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/page_aboutus_team_members"><span>Team Members</span></a><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/page_aboutus_special_thanks"><span>Spcial Thanks</span></a></li><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/page_aboutus_LT"><span>Logo and T-shirt</span></a></li><br />
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<h2> &nbsp;&nbsp;&nbsp;&nbsp;Would any of your project ideas raise safety issues in terms of: <br/><br />
• researcher safety, <br/><br />
• public safety, or <br/><br />
• environmental safety? </h2><br />
<br /><br /><br /><br />
<p>Actually no.</p><br />
<p>&nbsp;&nbsp;&nbsp;&nbsp;From the aspect of the project itself, there is no part in the iGEM 2011 SYSU-China would raise any safety issue in terms of researchers, the public or the environment. The bacteria we are working on are Escherichia coli BL21 and DH5α,the non-virulent mutants of Escherichia coli, which are widely accepted to be non-pathogenic and unlikely to survive in host tissues and cause disease. These are confirmed by R.M. La Ragione and M.J. Woodwad in their paper An investigation into the pathogenic properties of Escherichia coli strains BLR, BL21, DH5a and EQ1 <a href="http://ors.uchc.edu/bio/resources/pdf/3.6.1.A_colipath.pdf">(http://ors.uchc.edu/bio/resources/pdf/3.6.1.A_colipath.pdf)</a>. In addition, because of several years' operation of the two kinds of E.coli in the labs and no virulence report against human, we believe that these strains are harmless to researchers, the public, and the environment. From the aspect of our iGEM team members, each one was required to attend a pre-lab training conducted by graduate students and advisors both on experimental skills and safety instructions before he or she actually started the iGEM program in the lab. They trained us how to operate the fundamental experiments, and taught us the basic safety rules in the lab, such as how to use the toxic reagents. During the program, we wear gloves properly and disinfect tubes and plates after use. When our experiments involve the radiation-related operation, it will be carried out by the technician with radiation-usage permission. We also conform strictly to the established safety rules in the lab <a href="http://biosafety.sysu.edu.cn/administer/UploadFiles_9471/200804/2008041817410158.pdf">(http://biosafety.sysu.edu.cn/administer/UploadFiles_9471/200804/2008041817410158.pdf)</a>. </p><br />
<br />
<br /><br /><br /><br /><br />
<h2>&nbsp;&nbsp;&nbsp;&nbsp;Do any of the new BioBrick parts (or devices) that you made this year raise any safety issues? If yes, <br/><br />
• did you document these issues in the Registry? <br/><br />
• how did you manage to handle the safety issue? <br/><br />
• How could other teams learn from your experience? </h2><br />
<br /><br /><br /><br />
<p>&nbsp;&nbsp;&nbsp;&nbsp;No biobricks made by the SYSU-China team will raise any safety issues. The biobricks, such as recA, recN, cheZ, trkD and ag43, are all the natural parts of E.coli in natural environment, and commonly used for laboratory operation. Their function and functional mechanism are clearly known, and they are not associated with pathogenicity, infectivity, or toxicity, nor will they cause threats to environmental quality. Moreover, cheZ and ag43 are standard biobricks provided by iGEM authority, so the safety of these biobricks can be guaranteed.</p><br />
<br /><br /><br /><br /><br />
<h2> &nbsp;&nbsp;&nbsp;&nbsp;Is there a local biosafety group, committee, or review board at your institution? <br /><br />
• If yes, what does your local biosafety group think about your project? <br /><br />
• If no, which specific biosafety rules or guidelines do you have to consider in your country? </h2> <br />
<br /><br /><br /><br />
<p>&nbsp;&nbsp;&nbsp;&nbsp;In our university, Biosafety Committee of Sun Yat-Sen University is responsible for monitoring the safety of all researches on the campus. Their regulations are stipulated according to the WHO Laboratory biosafety manual, which is also conformed to by our country <a href="http://biosafety.sysu.edu.cn/Soft/UploadSoft/200804/2008042116071397.pdf">(http://biosafety.sysu.edu.cn/Soft/UploadSoft/200804/2008042116071397.pdf)</a>. </p><br />
<p>&nbsp;&nbsp;&nbsp;&nbsp;Before our team started the program, we have discussed about the safety issues of the whole project. The committee paid great attention on the radiation-related experiments, and emphasized that these experiments must be operated by the professional technician. Furthermore, the committee also requested that all procedures conducted in this project should be performed according to the rules stipulated by WHO. On the whole, they confirmed our project to be safe. With the surveillance of the Biosafety Committee of Sun Yat-sen University and the cautious operation of our members, the project will be of no harm to the researchers, the public or the environment.</p><br />
<br />
<br /><br /><br /><br /><br />
<h2>&nbsp;&nbsp;&nbsp;&nbsp;Do you have any other ideas how to deal with safety issues that could be useful for future iGEM competitions? How could parts, devices and systems be made even safer through biosafety engineering?</h2><br />
<br /><br /><br /><br />
<p>&nbsp;&nbsp;&nbsp;&nbsp;We suggest that the safety information of any part used in the iGEM competition, especially its functions, should be investigated clearly through literature search before the usage. If any part has any intention to raise safety issues, the iGEM team should refuse to use it and choose other alternatives. Additionally, iGEM teams should submit the safety report with their biobricks part to the iGEM authority, describing the safety issues of the biobricks part, which should also be examined by a professional third party. Thus the high-risk biobricks or parts with potential biosafety problems will be identified and abandoned before submitting. Through this way can the parts, devices and systems be safe. With measures above, the safety of the biobricks submitted can be guaranteed.<br />
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<p>From the 2011 iGEM team SYSU-China (2011)</p><br />
<p>Sun Yat-Sen University, Guangzhou, China</p><br />
<p>Address: 135# Xingang Rd.(W.), Haizhu Guangzhou, P.R.China</p><br />
<p><a href="http://www.sysu.edu.cn">visit the Sun Yat-sen university website</a></p><br />
<p>Thanks Apycom jQuery Menus and <a href="http://apycom.com/">visit their website</a><br />
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<li><a href="https://2011.igem.org/Team:SYSU-China/main_page_news"><span>NEWS</span></a></li><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/main_page_story"><span>STORY</span></a></li> <br />
<li><a href="https://2011.igem.org/Team:SYSU-China/main_page_project" class="parent"><span>PROJECT</span></a><br />
<div><br />
<ul><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/page_project_modules_construction"><span>Modules Construction</span></a><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/page_project_modules_verification"><span>Modules Verification</span></a></li><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/page_project_functional_construction"><span>Functional Verification</span></a></li><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/page_project_notes"><span>Notes</span></a></li><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/page_project_datapage"><span>Date Page</span></a></li><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/page_project_safety"><span>Safety</span></a></li><br />
</ul><br />
</div><br />
</li><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/main_page_human_practice" class="parent"><span>HUMAN PRACTICE</span></a><br />
<div><br />
<ul><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/page_human_practice_app"><span>App</span></a><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/page_human_practice_labcraft"><span>LabCraft Board Game</span></a></li><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/page_human_practice_workshop"><span>Workshop</span></a></li><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/page_human_practice_survey"><span>Survey</span></a></li><br />
</ul><br />
</div><br />
</li><br />
<br />
<li><a href="https://2011.igem.org/Team:SYSU-China/main_page_aboutus" class="parent"><span>ABOUT US</span></a><br />
<div><br />
<ul><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/page_aboutus_team_members"><span>Team Members</span></a><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/page_aboutus_special_thanks"><span>Spcial Thanks</span></a></li><br />
<li><a href="https://2011.igem.org/Team:SYSU-China/page_aboutus_LT"><span>Logo and T-shirt</span></a></li><br />
</ul><br />
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</li><br />
<br />
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<h2> &nbsp;&nbsp;&nbsp;&nbsp;Would any of your project ideas raise safety issues in terms of: <br/><br />
• researcher safety, <br/><br />
• public safety, or <br/><br />
• environmental safety? </h2><br />
<br /><br /><br /><br />
<p>Actually no.</p><br />
<p>&nbsp;&nbsp;&nbsp;&nbsp;From the aspect of the project itself, there is no part in the iGEM 2011 SYSU-China would raise any safety issue in terms of researchers, the public or the environment. The bacteria we are working on are Escherichia coli BL21 and DH5α,the non-virulent mutants of Escherichia coli, which are widely accepted to be non-pathogenic and unlikely to survive in host tissues and cause disease. These are confirmed by R.M. La Ragione and M.J. Woodwad in their paper An investigation into the pathogenic properties of Escherichia coli strains BLR, BL21, DH5a and EQ1 <a href="http://ors.uchc.edu/bio/resources/pdf/3.6.1.A_colipath.pdf">(http://ors.uchc.edu/bio/resources/pdf/3.6.1.A_colipath.pdf)</a>. In addition, because of several years' operation of the two kinds of E.coli in the labs and no virulence report against human, we believe that these strains are harmless to researchers, the public, and the environment. From the aspect of our iGEM team members, each one was required to attend a pre-lab training conducted by graduate students and advisors both on experimental skills and safety instructions before he or she actually started the iGEM program in the lab. They trained us how to operate the fundamental experiments, and taught us the basic safety rules in the lab, such as how to use the toxic reagents. During the program, we wear gloves properly and disinfect tubes and plates after use. When our experiments involve the radiation-related operation, it will be carried out by the technician with radiation-usage permission. We also conform strictly to the established safety rules in the lab <a href="http://biosafety.sysu.edu.cn/administer/UploadFiles_9471/200804/2008041817410158.pdf">(http://biosafety.sysu.edu.cn/administer/UploadFiles_9471/200804/2008041817410158.pdf)</a>. </p><br />
<br />
<br /><br /><br /><br /><br />
<h2>&nbsp;&nbsp;&nbsp;&nbsp;Do any of the new BioBrick parts (or devices) that you made this year raise any safety issues? If yes, <br/><br />
• did you document these issues in the Registry? <br/><br />
• how did you manage to handle the safety issue? <br/><br />
• How could other teams learn from your experience? </h2><br />
<br /><br /><br /><br />
<p>&nbsp;&nbsp;&nbsp;&nbsp;No biobricks made by the SYSU-China team will raise any safety issues. The biobricks, such as recA, recN, cheZ, trkD and ag43, are all the natural parts of E.coli in natural environment, and commonly used for laboratory operation. Their function and functional mechanism are clearly known, and they are not associated with pathogenicity, infectivity, or toxicity, nor will they cause threats to environmental quality. Moreover, cheZ and ag43 are standard biobricks provided by iGEM authority, so the safety of these biobricks can be guaranteed.</p><br />
<br /><br /><br /><br /><br />
<h2> &nbsp;&nbsp;&nbsp;&nbsp;Is there a local biosafety group, committee, or review board at your institution? <br /><br />
• If yes, what does your local biosafety group think about your project? <br /><br />
• If no, which specific biosafety rules or guidelines do you have to consider in your country? </h2> <br />
<br /><br /><br /><br />
<p>&nbsp;&nbsp;&nbsp;&nbsp;In our university, Biosafety Committee of Sun Yat-Sen University is responsible for monitoring the safety of all researches on the campus. Their regulations are stipulated according to the WHO Laboratory biosafety manual, which is also conformed to by our country <a href="http://biosafety.sysu.edu.cn/Soft/UploadSoft/200804/2008042116071397.pdf">(http://biosafety.sysu.edu.cn/Soft/UploadSoft/200804/2008042116071397.pdf)</a>. </p><br />
<p>&nbsp;&nbsp;&nbsp;&nbsp;Before our team started the program, we have discussed about the safety issues of the whole project. The committee paid great attention on the radiation-related experiments, and emphasized that these experiments must be operated by the professional technician. Furthermore, the committee also requested that all procedures conducted in this project should be performed according to the rules stipulated by WHO. On the whole, they confirmed our project to be safe. With the surveillance of the Biosafety Committee of Sun Yat-sen University and the cautious operation of our members, the project will be of no harm to the researchers, the public or the environment.</p><br />
<br />
<br /><br /><br /><br /><br />
<h2>&nbsp;&nbsp;&nbsp;&nbsp;Do you have any other ideas how to deal with safety issues that could be useful for future iGEM competitions? How could parts, devices and systems be made even safer through biosafety engineering?</h2><br />
<br /><br /><br /><br />
<p>&nbsp;&nbsp;&nbsp;&nbsp;We suggest that the safety information of any part used in the iGEM competition, especially its functions, should be investigated clearly through literature search before the usage. If any part has any intention to raise safety issues, the iGEM team should refuse to use it and choose other alternatives. Additionally, iGEM teams should submit the safety report with their biobricks part to the iGEM authority, describing the safety issues of the biobricks part, which should also be examined by a professional third party. Thus the high-risk biobricks or parts with potential biosafety problems will be identified and abandoned before submitting. Through this way can the parts, devices and systems be safe. With measures above, the safety of the biobricks submitted can be guaranteed.<br />
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<p>From the 2011 iGEM team SYSU-China (2011)</p><br />
<p>Sun Yat-Sen University, Guangzhou, China</p><br />
<p>Address: 135# Xingang Rd.(W.), Haizhu Guangzhou, P.R.China</p><br />
<p><a href="http://www.sysu.edu.cn">visit the Sun Yat-sen university website</a></p><br />
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<li><a href="https://2011.igem.org/Team:SYSU-China/page_project_modules_construction"><span>Modules Construction</span></a><br />
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<h2> &nbsp;&nbsp;&nbsp;&nbsp;Would any of your project ideas raise safety issues in terms of: <br/><br />
• researcher safety, <br/><br />
• public safety, or <br/><br />
• environmental safety? </h2><br />
<br /><br /><br /><br />
<p>Actually no.</p><br />
<p>&nbsp;&nbsp;&nbsp;&nbsp;From the aspect of the project itself, there is no part in the iGEM 2011 SYSU-China would raise any safety issue in terms of researchers, the public or the environment. The bacteria we are working on are Escherichia coli BL21 and DH5α,the non-virulent mutants of Escherichia coli, which are widely accepted to be non-pathogenic and unlikely to survive in host tissues and cause disease. These are confirmed by R.M. La Ragione and M.J. Woodwad in their paper An investigation into the pathogenic properties of Escherichia coli strains BLR, BL21, DH5a and EQ1 <a href="http://ors.uchc.edu/bio/resources/pdf/3.6.1.A_colipath.pdf">(http://ors.uchc.edu/bio/resources/pdf/3.6.1.A_colipath.pdf)</a>. In addition, because of several years' operation of the two kinds of E.coli in the labs and no virulence report against human, we believe that these strains are harmless to researchers, the public, and the environment. From the aspect of our iGEM team members, each one was required to attend a pre-lab training conducted by graduate students and advisors both on experimental skills and safety instructions before he or she actually started the iGEM program in the lab. They trained us how to operate the fundamental experiments, and taught us the basic safety rules in the lab, such as how to use the toxic reagents. During the program, we wear gloves properly and disinfect tubes and plates after use. When our experiments involve the radiation-related operation, it will be carried out by the technician with radiation-usage permission. We also conform strictly to the established safety rules in the lab <a href="http://biosafety.sysu.edu.cn/administer/UploadFiles_9471/200804/2008041817410158.pdf">(http://biosafety.sysu.edu.cn/administer/UploadFiles_9471/200804/2008041817410158.pdf)</a>. </p><br />
<br />
<br /><br /><br /><br /><br />
<h2>&nbsp;&nbsp;&nbsp;&nbsp;Do any of the new BioBrick parts (or devices) that you made this year raise any safety issues? If yes, <br/><br />
• did you document these issues in the Registry? <br/><br />
• how did you manage to handle the safety issue? <br/><br />
• How could other teams learn from your experience? </h2><br />
<br /><br /><br /><br />
<p>&nbsp;&nbsp;&nbsp;&nbsp;No biobricks made by the SYSU-China team will raise any safety issues. The biobricks, such as recA, recN, cheZ, trkD and ag43, are all the natural parts of E.coli in natural environment, and commonly used for laboratory operation. Their function and functional mechanism are clearly known, and they are not associated with pathogenicity, infectivity, or toxicity, nor will they cause threats to environmental quality. Moreover, cheZ and ag43 are standard biobricks provided by iGEM authority, so the safety of these biobricks can be guaranteed.</p><br />
<br /><br /><br /><br /><br />
<h2> &nbsp;&nbsp;&nbsp;&nbsp;Is there a local biosafety group, committee, or review board at your institution? <br /><br />
• If yes, what does your local biosafety group think about your project? <br /><br />
• If no, which specific biosafety rules or guidelines do you have to consider in your country? </h2> <br />
<br /><br /><br /><br />
<p>&nbsp;&nbsp;&nbsp;&nbsp;In our university, Biosafety Committee of Sun Yat-Sen University is responsible for monitoring the safety of all researches on the campus. Their regulations are stipulated according to the WHO Laboratory biosafety manual, which is also conformed to by our country <a href="http://biosafety.sysu.edu.cn/Soft/UploadSoft/200804/2008042116071397.pdf">(http://biosafety.sysu.edu.cn/Soft/UploadSoft/200804/2008042116071397.pdf)</a>. </p><br />
<p>&nbsp;&nbsp;&nbsp;&nbsp;Before our team started the program, we have discussed about the safety issues of the whole project. The committee paid great attention on the radiation-related experiments, and emphasized that these experiments must be operated by the professional technician. Furthermore, the committee also requested that all procedures conducted in this project should be performed according to the rules stipulated by WHO. On the whole, they confirmed our project to be safe. With the surveillance of the Biosafety Committee of Sun Yat-sen University and the cautious operation of our members, the project will be of no harm to the researchers, the public or the environment.</p><br />
<br />
<br /><br /><br /><br /><br />
<h2>&nbsp;&nbsp;&nbsp;&nbsp;Do you have any other ideas how to deal with safety issues that could be useful for future iGEM competitions? How could parts, devices and systems be made even safer through biosafety engineering?</h2><br />
<br /><br /><br /><br />
<p>&nbsp;&nbsp;&nbsp;&nbsp;We suggest that the safety information of any part used in the iGEM competition, especially its functions, should be investigated clearly through literature search before the usage. If any part has any intention to raise safety issues, the iGEM team should refuse to use it and choose other alternatives. Additionally, iGEM teams should submit the safety report with their biobricks part to the iGEM authority, describing the safety issues of the biobricks part, which should also be examined by a professional third party. Thus the high-risk biobricks or parts with potential biosafety problems will be identified and abandoned before submitting. Through this way can the parts, devices and systems be safe. With measures above, the safety of the biobricks submitted can be guaranteed.<br />
</p><br />
<br />
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<p>From the 2011 iGEM team SYSU-China (2011)</p><br />
<p>Sun Yat-Sen University, Guangzhou, China</p><br />
<p>Address: 135# Xingang Rd.(W.), Haizhu Guangzhou, P.R.China</p><br />
<p><a href="http://www.sysu.edu.cn">visit the Sun Yat-sen university website</a></p><br />
<p>Thanks Apycom jQuery Menus and <a href="http://apycom.com/">visit their website</a><br />
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</html></div>Cambihttp://2011.igem.org/Team:SYSU-China/NotebookTeam:SYSU-China/Notebook2011-10-05T14:58:19Z<p>Cambi: </p>
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<br />
<h2> &nbsp;&nbsp;&nbsp;&nbsp;Would any of your project ideas raise safety issues in terms of: <br/><br />
• researcher safety, <br/><br />
• public safety, or <br/><br />
• environmental safety? </h2><br />
<br /><br /><br /><br />
<p>Actually no.</p><br />
<p>&nbsp;&nbsp;&nbsp;&nbsp;From the aspect of the project itself, there is no part in the iGEM 2011 SYSU-China would raise any safety issue in terms of researchers, the public or the environment. The bacteria we are working on are Escherichia coli BL21 and DH5α,the non-virulent mutants of Escherichia coli, which are widely accepted to be non-pathogenic and unlikely to survive in host tissues and cause disease. These are confirmed by R.M. La Ragione and M.J. Woodwad in their paper An investigation into the pathogenic properties of Escherichia coli strains BLR, BL21, DH5a and EQ1 <a href="http://ors.uchc.edu/bio/resources/pdf/3.6.1.A_colipath.pdf">(http://ors.uchc.edu/bio/resources/pdf/3.6.1.A_colipath.pdf)</a>. In addition, because of several years' operation of the two kinds of E.coli in the labs and no virulence report against human, we believe that these strains are harmless to researchers, the public, and the environment. From the aspect of our iGEM team members, each one was required to attend a pre-lab training conducted by graduate students and advisors both on experimental skills and safety instructions before he or she actually started the iGEM program in the lab. They trained us how to operate the fundamental experiments, and taught us the basic safety rules in the lab, such as how to use the toxic reagents. During the program, we wear gloves properly and disinfect tubes and plates after use. When our experiments involve the radiation-related operation, it will be carried out by the technician with radiation-usage permission. We also conform strictly to the established safety rules in the lab <a href="http://biosafety.sysu.edu.cn/administer/UploadFiles_9471/200804/2008041817410158.pdf">(http://biosafety.sysu.edu.cn/administer/UploadFiles_9471/200804/2008041817410158.pdf)</a>. </p><br />
<br />
<br /><br /><br /><br /><br />
<h2>&nbsp;&nbsp;&nbsp;&nbsp;Do any of the new BioBrick parts (or devices) that you made this year raise any safety issues? If yes, <br/><br />
• did you document these issues in the Registry? <br/><br />
• how did you manage to handle the safety issue? <br/><br />
• How could other teams learn from your experience? </h2><br />
<br /><br /><br /><br />
<p>&nbsp;&nbsp;&nbsp;&nbsp;No biobricks made by the SYSU-China team will raise any safety issues. The biobricks, such as recA, recN, cheZ, trkD and ag43, are all the natural parts of E.coli in natural environment, and commonly used for laboratory operation. Their function and functional mechanism are clearly known, and they are not associated with pathogenicity, infectivity, or toxicity, nor will they cause threats to environmental quality. Moreover, cheZ and ag43 are standard biobricks provided by iGEM authority, so the safety of these biobricks can be guaranteed.</p><br />
<br /><br /><br /><br /><br />
<h2> &nbsp;&nbsp;&nbsp;&nbsp;Is there a local biosafety group, committee, or review board at your institution? <br /><br />
• If yes, what does your local biosafety group think about your project? <br /><br />
• If no, which specific biosafety rules or guidelines do you have to consider in your country? </h2> <br />
<br /><br /><br /><br />
<p>&nbsp;&nbsp;&nbsp;&nbsp;In our university, Biosafety Committee of Sun Yat-Sen University is responsible for monitoring the safety of all researches on the campus. Their regulations are stipulated according to the WHO Laboratory biosafety manual, which is also conformed to by our country <a href="http://biosafety.sysu.edu.cn/Soft/UploadSoft/200804/2008042116071397.pdf">(http://biosafety.sysu.edu.cn/Soft/UploadSoft/200804/2008042116071397.pdf)</a>. </p><br />
<p>&nbsp;&nbsp;&nbsp;&nbsp;Before our team started the program, we have discussed about the safety issues of the whole project. The committee paid great attention on the radiation-related experiments, and emphasized that these experiments must be operated by the professional technician. Furthermore, the committee also requested that all procedures conducted in this project should be performed according to the rules stipulated by WHO. On the whole, they confirmed our project to be safe. With the surveillance of the Biosafety Committee of Sun Yat-sen University and the cautious operation of our members, the project will be of no harm to the researchers, the public or the environment.</p><br />
<br />
<br /><br /><br /><br /><br />
<h2>&nbsp;&nbsp;&nbsp;&nbsp;Do you have any other ideas how to deal with safety issues that could be useful for future iGEM competitions? How could parts, devices and systems be made even safer through biosafety engineering?</h2><br />
<br /><br /><br /><br />
<p>&nbsp;&nbsp;&nbsp;&nbsp;We suggest that the safety information of any part used in the iGEM competition, especially its functions, should be investigated clearly through literature search before the usage. If any part has any intention to raise safety issues, the iGEM team should refuse to use it and choose other alternatives. Additionally, iGEM teams should submit the safety report with their biobricks part to the iGEM authority, describing the safety issues of the biobricks part, which should also be examined by a professional third party. Thus the high-risk biobricks or parts with potential biosafety problems will be identified and abandoned before submitting. Through this way can the parts, devices and systems be safe. With measures above, the safety of the biobricks submitted can be guaranteed.<br />
</p><br />
<br />
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<p>From the 2011 iGEM team SYSU-China (2011)</p><br />
<p>Sun Yat-Sen University, Guangzhou, China</p><br />
<p>Address: 135# Xingang Rd.(W.), Haizhu Guangzhou, P.R.China</p><br />
<p><a href="http://www.sysu.edu.cn">visit the Sun Yat-sen university website</a></p><br />
<p>Thanks Apycom jQuery Menus and <a href="http://apycom.com/">visit their website</a><br />
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<h2> &nbsp;&nbsp;&nbsp;&nbsp;Would any of your project ideas raise safety issues in terms of: <br/><br />
• researcher safety, <br/><br />
• public safety, or <br/><br />
• environmental safety? </h2><br />
<br /><br /><br /><br />
<p>Actually no.</p><br />
<p>&nbsp;&nbsp;&nbsp;&nbsp;From the aspect of the project itself, there is no part in the iGEM 2011 SYSU-China would raise any safety issue in terms of researchers, the public or the environment. The bacteria we are working on are Escherichia coli BL21 and DH5α,the non-virulent mutants of Escherichia coli, which are widely accepted to be non-pathogenic and unlikely to survive in host tissues and cause disease. These are confirmed by R.M. La Ragione and M.J. Woodwad in their paper An investigation into the pathogenic properties of Escherichia coli strains BLR, BL21, DH5a and EQ1 <a href="http://ors.uchc.edu/bio/resources/pdf/3.6.1.A_colipath.pdf">(http://ors.uchc.edu/bio/resources/pdf/3.6.1.A_colipath.pdf)</a>. In addition, because of several years' operation of the two kinds of E.coli in the labs and no virulence report against human, we believe that these strains are harmless to researchers, the public, and the environment. From the aspect of our iGEM team members, each one was required to attend a pre-lab training conducted by graduate students and advisors both on experimental skills and safety instructions before he or she actually started the iGEM program in the lab. They trained us how to operate the fundamental experiments, and taught us the basic safety rules in the lab, such as how to use the toxic reagents. During the program, we wear gloves properly and disinfect tubes and plates after use. When our experiments involve the radiation-related operation, it will be carried out by the technician with radiation-usage permission. We also conform strictly to the established safety rules in the lab <a href="http://biosafety.sysu.edu.cn/administer/UploadFiles_9471/200804/2008041817410158.pdf">(http://biosafety.sysu.edu.cn/administer/UploadFiles_9471/200804/2008041817410158.pdf)</a>. </p><br />
<br />
<br /><br /><br /><br /><br />
<h2>&nbsp;&nbsp;&nbsp;&nbsp;Do any of the new BioBrick parts (or devices) that you made this year raise any safety issues? If yes, <br/><br />
• did you document these issues in the Registry? <br/><br />
• how did you manage to handle the safety issue? <br/><br />
• How could other teams learn from your experience? </h2><br />
<br /><br /><br /><br />
<p>&nbsp;&nbsp;&nbsp;&nbsp;No biobricks made by the SYSU-China team will raise any safety issues. The biobricks, such as recA, recN, cheZ, trkD and ag43, are all the natural parts of E.coli in natural environment, and commonly used for laboratory operation. Their function and functional mechanism are clearly known, and they are not associated with pathogenicity, infectivity, or toxicity, nor will they cause threats to environmental quality. Moreover, cheZ and ag43 are standard biobricks provided by iGEM authority, so the safety of these biobricks can be guaranteed.</p><br />
<br /><br /><br /><br /><br />
<h2> &nbsp;&nbsp;&nbsp;&nbsp;Is there a local biosafety group, committee, or review board at your institution? <br /><br />
• If yes, what does your local biosafety group think about your project? <br /><br />
• If no, which specific biosafety rules or guidelines do you have to consider in your country? </h2> <br />
<br /><br /><br /><br />
<p>&nbsp;&nbsp;&nbsp;&nbsp;In our university, Biosafety Committee of Sun Yat-Sen University is responsible for monitoring the safety of all researches on the campus. Their regulations are stipulated according to the WHO Laboratory biosafety manual, which is also conformed to by our country <a href="http://biosafety.sysu.edu.cn/Soft/UploadSoft/200804/2008042116071397.pdf">(http://biosafety.sysu.edu.cn/Soft/UploadSoft/200804/2008042116071397.pdf)</a>. </p><br />
<p>&nbsp;&nbsp;&nbsp;&nbsp;Before our team started the program, we have discussed about the safety issues of the whole project. The committee paid great attention on the radiation-related experiments, and emphasized that these experiments must be operated by the professional technician. Furthermore, the committee also requested that all procedures conducted in this project should be performed according to the rules stipulated by WHO. On the whole, they confirmed our project to be safe. With the surveillance of the Biosafety Committee of Sun Yat-sen University and the cautious operation of our members, the project will be of no harm to the researchers, the public or the environment.</p><br />
<br />
<br /><br /><br /><br /><br />
<h2>&nbsp;&nbsp;&nbsp;&nbsp;Do you have any other ideas how to deal with safety issues that could be useful for future iGEM competitions? How could parts, devices and systems be made even safer through biosafety engineering?</h2><br />
<br /><br /><br /><br />
<p>&nbsp;&nbsp;&nbsp;&nbsp;We suggest that the safety information of any part used in the iGEM competition, especially its functions, should be investigated clearly through literature search before the usage. If any part has any intention to raise safety issues, the iGEM team should refuse to use it and choose other alternatives. Additionally, iGEM teams should submit the safety report with their biobricks part to the iGEM authority, describing the safety issues of the biobricks part, which should also be examined by a professional third party. Thus the high-risk biobricks or parts with potential biosafety problems will be identified and abandoned before submitting. Through this way can the parts, devices and systems be safe. With measures above, the safety of the biobricks submitted can be guaranteed.<br />
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<p>Sun Yat-Sen University, Guangzhou, China</p><br />
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<p>From the 2011 iGEM team SYSU-China (2011)</p><br />
<p>Sun Yat-Sen University, Guangzhou, China</p><br />
<p>Address: 135# Xingang Rd.(W.), Haizhu Guangzhou, P.R.China</p><br />
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