Safety.html

From 2011.igem.org

(Difference between revisions)
 
(4 intermediate revisions not shown)
Line 1: Line 1:
-
<!DOCTYPE html PUBLIC "-//W3C//DTD XHTML 1.0 Transitional//EN" "http://www.w3.org/TR/xhtml1/DTD/xhtml1-transitional.dtd">
 
<html xmlns="http://www.w3.org/1999/xhtml">
<html xmlns="http://www.w3.org/1999/xhtml">
<head>
<head>
<meta http-equiv="Content-Type" content="text/html; charset=utf-8" />
<meta http-equiv="Content-Type" content="text/html; charset=utf-8" />
-
<title>Fatih_Turkey</title>
 
<style type="text/css">
<style type="text/css">
<!--
<!--
Line 31: Line 29:
<body>
<body>
-
<table width="969" border="0" align="center">
+
<table width="967" border="0" align="center">
   <tr>
   <tr>
-
     <td width="963" height="226" align="center" valign="middle" background="renkli.jpg">&nbsp;</td>
+
     <td width="961" height="226" align="center" valign="middle" background="https://static.igem.org/mediawiki/2011/0/02/Renkli.jpg">&nbsp;</td>
   </tr>
   </tr>
   <tr>
   <tr>
-
     <td height="53" bgcolor="#FFFFFF"><table width="968" border="0" cellspacing="10" bgcolor="#FFFFFF">
+
     <td height="53" bgcolor="#FFFFFF"><table width="961" border="0" cellspacing="10" bgcolor="#FFFFFF">
         <tr>
         <tr>
-
           <td width="187" align="center" valign="middle" bgcolor="#990000"><a href="index.html"><span class="style3">HOME</span></a></td>
+
           <td width="177" align="center" valign="middle" bgcolor="#990000"><a href="https://2011.igem.org/Team:Fatih_Turkey"><span class="style3">HOME</span></a></td>
-
           <td width="226" align="center" valign="middle" bgcolor="#695E0E"><a href="canvastown.html"><span class="style3">CANVAS&nbsp; TOWN</span></a></td>
+
           <td width="236" align="center" valign="middle" bgcolor="#695E0E"><a href="canvastown.html"><span class="style3">CANVAS&nbsp; TOWN</span></a></td>
-
           <td width="258" align="center" valign="middle" bgcolor="#1D3551"><a href="therainbowgraveyard.html"><span class="style3">THE RAINBOW GRAVEYARD</span></a></td>
+
           <td width="271" align="center" valign="middle" bgcolor="#1D3551"><a href="therainbowgraveyard.html"><span class="style3">THE RAINBOW GRAVEYARD</span></a></td>
-
           <td width="239" align="center" valign="middle" bgcolor="#960C47"><a href="notebook.html"><span class="style3">&nbsp;NOTEBOOK</span></a></td>
+
           <td width="220" align="center" valign="middle" bgcolor="#960C47"><a href="notebook.html"><span class="style3">&nbsp;NOTEBOOK</span></a></td>
         </tr>
         </tr>
         <tr>
         <tr>
Line 49: Line 47:
   </tr>
   </tr>
   <tr>
   <tr>
-
     <td height="778" align="center" valign="top" bgcolor="#FFFFFF"><p>&nbsp;</p>
+
     <td height="778" align="center" valign="top" bgcolor="#FFFFFF"><table width="799" border="0">
-
      <table width="799" border="0">
+
       <tr>
       <tr>
-
         <td width="793" bgcolor="#FFFFFF"><p>In our history, our hero is B.subtilis because of capability of catching E.coli, especially gram-negative bacteria species presenting LPS layer with the help of synthesized Limulus anti-LPS factor (LALF) onto the biofilm. On the other hand, in The Canvas Town, all species of our project story including B.subtilis police squad and E.coli gang can synthesize Reflectin protein transferred from cephalopod.</p>
+
         <td width="793" height="140" bgcolor="#FFFFFF"><p align="center"><strong> </strong><strong>FAT</strong><strong>IH UNIVERSITY MEDICAL SCHOOL</strong><br />
-
           <p>Here, we will answer the safety questions to tell how we will take precautions in our lab experiments and in usage of our project in public.</p>
+
              <strong>ANSWERS  TO NEW SAFETY AND SECURITY QUESTIONS</strong><strong> </strong></p>
-
           <p><strong>1. Would any of your project ideas raise safety issues in terms of:</strong> <br />
+
          <p><strong>1. Would the materials used in your project and/or your final  product pose:</strong><br />
-
              <a name="Researcher_Safety" id="Researcher_Safety"></a>We have one plan to raise safety issues when studying with B.subtilis. Under stress conditions, <em>B. subtilis</em> will form s
+
              <strong>a. Risks to the safety and health of team members or others  in the lab?</strong><br />
 +
            Actually few hazardous  chemicals and solutions are used in some lab procedures such as gel preparation  and electrophoresis.  However these chemicals and solutions are used according to the safety rules of  the laboratory with care and caution. All the members were trained for safety  regulations of the laboratory as well as toxicity of the chemicals and  solutions before starting the current project.  </p>
 +
          <p><strong>b. Risks to the safety and health of the general public if  released by design or accident?</strong><br />
 +
            When released by accident,  our parts and materials actually cause no negative damage to the general  public. B.subtilis is known not to be associated with disease process in humans  in regular conditions and thus are supposed to be benign by nature and present  no danger to anyone. Only under stress conditions,&nbsp;B. subtilis&nbsp;may  form spores and these spores are resistant versions of single cells.  While&nbsp;B. subtilis&nbsp;itself isn't pathogenic, its spores may be  infectious if inhaled. But all of our team members are trained for safety of the  laboratory rules and use mask and gloves when dealing with bacteria. Besides  all experiments are performed in synthetic laboratory designated by Fatih  University Medical School and no material is carried out of the laboratory. However  to increase public safety of our project, in case of accident, our plan is to  destroy particles including B.subtilis by cleaning the biofilm from the spores and  treating with Fenton reagent containing dissolved oxygen and other  constituents.</p>
 +
          <p><strong>c. Risks to environmental quality if released by design or  accident?</strong></p>
 +
          <p>Bacillus subtilis strain  3610 is a wild type strain that is already widespread in the environment. B.  subtilis strain 168  is a derivative  strain of the 3610 and has disabling auxotrophs mutations  that makes it even less likely to colonize or  cause harm to human or animal health. Our experiment does not include any  modifications, which enhance their ability to survive or disseminate. Thus,  there is no specific environmental risk associated with use of the Bacillus subtilis  strains except its spore formation. In case of accident, cleaning and treating  the spores with Fenton reagent as mentioned above provide environmental safety.  Besides all procedures are performed in a specified laboratory.<br />
 +
            E. Coli strains TOP10 and  BL1 have very limited ability to survive outside the laboratory so that it  would be unable to survive or disseminate. Therefore, there is no specific  environmental risk associated with the E. coli strains. <br />
 +
            All bacterial waste are  kept in 10% bleaching solution for one day, then are autoclaved to be  sterilized. </p>
 +
          <p><strong>d. Risks to security through malicious misuse by individuals,  groups or states?</strong><br />
 +
            Please explain your  responses (whether yes or no) to these questions. Specifically, are any parts  or devices in your project associated with (or known to cause):<br />
 +
            - pathogenicity, infectivity, or toxicity? No <br />
 +
            - threats to environmental  quality? No<br />
 +
            - security concerns? No<br />
 +
            As explained below:<br />
 +
            Nothing could be used  maliciously in our project. The materials we use are commonly used in  experimental procedures in various laboratories all over the world and all are harmless  and do not pose any threat to environment and public as well as security  concerns. <br />
 +
            Throughout the project, we  used the Escherichia coli strains Top10 and BL1, Bacillus subtilis strains 3610  and 168. Wild-type E. coli is classified as a hazard group 2 pathogen by the UK  Advisory Committee on the Dangerous Pathogens (ACDP) and Wild-type Bacillus  subtilis (i.e. strain 3610) is classified as hazard group 1 organism by the  ACDP and its derivative B. subtilis strain 168 has disabling auxotrophs  mutations (e.g. conferring a requirement for tryptophan, Zeigler DR et al, 2008.  J of Bacteriology) that makes it even less likely to colonize or cause harm to  human or animal health. B.subtilis is at level 1 biosafety according to World  Health Organization (WHO).</p>
 +
           <p><strong>2. If your response to any of the questions above is yes:</strong><br />
 +
              <strong> a. Explain how you  addressed these issues in project design and while conducting laboratory work. </strong><br />
 +
              <strong>b. Describe and document safety, security, health and/or  environmental issues as you submit your parts to the Registry</strong></p>
 +
          <p><strong>3. Under what biosafety provisions will / do you operate?</strong></p>
 +
          <p><strong>a. Does your institution have its own biosafety rules and if  so what are they? Provide a link to them online if possible.</strong><br />
 +
            Yes, our institution has  its own biosafety rules. Rules for laboratory use, general principles,  prevention from hazardous materials and application of emergency intervention  in case of accident are included. The web link is given as (<a href="http://www.fatihmed.edu.tr/icerik/guvenlikkilavuzu.php">http://www.fatihmed.edu.tr/icerik/guvenlikkilavuzu.php</a>). </p>
 +
          <p><strong>b. Does your institution have an Institutional Biosafety Committee  or equivalent group? If yes, have you discussed your project with them?  Describe any concerns or changes that were made based on this review</strong> </p>
 +
          <p>Yes. In Fatih University  Medical School, Laboratory and Patient-Employee Safety Committee is responsible  for control as well as biosafety of laboratories and safety of patients and  employees. This committee works under one of the vice medical director of Fatih  University Hospital, Prof. Dr. Mehmet Gunduz. Form on safety rules of Fatih  University Medical School Laboratory use was filled in as required. We  discussed our project with Prof. Dr. Mehmet Gunduz. Safety and security issues  are found sufficient enough that no change is considered as necessary. (Further  questions can be directed to Prof. Gunduz, tel: +90-312-203 5103, mgunduz@fatih.edu.tr).</p>
 +
           <p><strong>c.Will / did you receive any biosafety and/or lab training  before beginning your project? If so, describe this training.</strong></p>
 +
          <p>Yes, we did. Our advisor  provided us biosafety and lab training before starting of our project. In the  training, general safety rules of laboratory use, prevention from hazardous  chemicals and solutions as well as emergency intervention in case of accident  were included.  </p>
 +
          <p><strong>d. Does your country have national biosafety regulations or  guidelines? If so, provide a link to them online if possible.</strong></p>
 +
          <p>Yes, Turkey has national  biosafety regulations and the link is given as (<a href="http://www.tbbdm.gov.tr/Home/BioSafetyCouncilHome/BioSafetyCouncilHomeChoose.aspx">http://www.tbbdm.gov.tr/Home/BioSafetyCouncilHome/BioSafetyCouncilHomeChoose.aspx</a>). </p>
 +
          <p><strong>4. OPTIONAL QUESTION: Do you have other ideas on how to deal with  safety or security issues that could be useful for future iGEM competitions?  How could parts, devices and systems be made even safer through biosafety  engineering?</strong></p>
 +
          <p>First, IGEM committee may  prepare a lab safety acknowledgement form like OSHA form. This form can be  required to be filled in by all members. This will allow us to confirm whether  members of the teams are informed about safety issues.<br />
 +
            Second, a new prize  related with security and safety issues may take place in the judging procedure  separated from “Best Human Practices” prize. Existence of “Best Safety Part” or  “Best Safety Application” can emphasize the importance of the safety issues and  inspire iGEM teams to work on these issues and may give a chance to develop new  ideas, to interrogate current safety and security applications in order to  improve them.<br />
 +
          Third, iGEM committee may  organize a webinar to inform the participants about safety and security in  labs. Webinar can be done at the beginning of the experiments. Mentor scientists  may talk about their experiences and mention some tricks about safety issues.</p></td>
 +
      </tr>
 +
    </table>   
 +
    <p>&nbsp;</p>
 +
    </td>
 +
  </tr>
 +
</table>
 +
</body>
 +
</html>

Latest revision as of 05:10, 25 August 2011

 
HOME CANVAS  TOWN THE RAINBOW GRAVEYARD  NOTEBOOK
SAFETY

 FATIH UNIVERSITY MEDICAL SCHOOL
ANSWERS TO NEW SAFETY AND SECURITY QUESTIONS

1. Would the materials used in your project and/or your final product pose:
a. Risks to the safety and health of team members or others in the lab?
Actually few hazardous chemicals and solutions are used in some lab procedures such as gel preparation and electrophoresis. However these chemicals and solutions are used according to the safety rules of the laboratory with care and caution. All the members were trained for safety regulations of the laboratory as well as toxicity of the chemicals and solutions before starting the current project.  

b. Risks to the safety and health of the general public if released by design or accident?
When released by accident, our parts and materials actually cause no negative damage to the general public. B.subtilis is known not to be associated with disease process in humans in regular conditions and thus are supposed to be benign by nature and present no danger to anyone. Only under stress conditions, B. subtilis may form spores and these spores are resistant versions of single cells. While B. subtilis itself isn't pathogenic, its spores may be infectious if inhaled. But all of our team members are trained for safety of the laboratory rules and use mask and gloves when dealing with bacteria. Besides all experiments are performed in synthetic laboratory designated by Fatih University Medical School and no material is carried out of the laboratory. However to increase public safety of our project, in case of accident, our plan is to destroy particles including B.subtilis by cleaning the biofilm from the spores and treating with Fenton reagent containing dissolved oxygen and other constituents.

c. Risks to environmental quality if released by design or accident?

Bacillus subtilis strain 3610 is a wild type strain that is already widespread in the environment. B. subtilis strain 168  is a derivative strain of the 3610 and has disabling auxotrophs mutations  that makes it even less likely to colonize or cause harm to human or animal health. Our experiment does not include any modifications, which enhance their ability to survive or disseminate. Thus, there is no specific environmental risk associated with use of the Bacillus subtilis strains except its spore formation. In case of accident, cleaning and treating the spores with Fenton reagent as mentioned above provide environmental safety. Besides all procedures are performed in a specified laboratory.
E. Coli strains TOP10 and BL1 have very limited ability to survive outside the laboratory so that it would be unable to survive or disseminate. Therefore, there is no specific environmental risk associated with the E. coli strains.
All bacterial waste are kept in 10% bleaching solution for one day, then are autoclaved to be sterilized.

d. Risks to security through malicious misuse by individuals, groups or states?
Please explain your responses (whether yes or no) to these questions. Specifically, are any parts or devices in your project associated with (or known to cause):
- pathogenicity, infectivity, or toxicity? No
- threats to environmental quality? No
- security concerns? No
As explained below:
Nothing could be used maliciously in our project. The materials we use are commonly used in experimental procedures in various laboratories all over the world and all are harmless and do not pose any threat to environment and public as well as security concerns.
Throughout the project, we used the Escherichia coli strains Top10 and BL1, Bacillus subtilis strains 3610 and 168. Wild-type E. coli is classified as a hazard group 2 pathogen by the UK Advisory Committee on the Dangerous Pathogens (ACDP) and Wild-type Bacillus subtilis (i.e. strain 3610) is classified as hazard group 1 organism by the ACDP and its derivative B. subtilis strain 168 has disabling auxotrophs mutations (e.g. conferring a requirement for tryptophan, Zeigler DR et al, 2008. J of Bacteriology) that makes it even less likely to colonize or cause harm to human or animal health. B.subtilis is at level 1 biosafety according to World Health Organization (WHO).

2. If your response to any of the questions above is yes:
 a. Explain how you addressed these issues in project design and while conducting laboratory work.
b. Describe and document safety, security, health and/or environmental issues as you submit your parts to the Registry

3. Under what biosafety provisions will / do you operate?

a. Does your institution have its own biosafety rules and if so what are they? Provide a link to them online if possible.
Yes, our institution has its own biosafety rules. Rules for laboratory use, general principles, prevention from hazardous materials and application of emergency intervention in case of accident are included. The web link is given as (http://www.fatihmed.edu.tr/icerik/guvenlikkilavuzu.php).

b. Does your institution have an Institutional Biosafety Committee or equivalent group? If yes, have you discussed your project with them? Describe any concerns or changes that were made based on this review

Yes. In Fatih University Medical School, Laboratory and Patient-Employee Safety Committee is responsible for control as well as biosafety of laboratories and safety of patients and employees. This committee works under one of the vice medical director of Fatih University Hospital, Prof. Dr. Mehmet Gunduz. Form on safety rules of Fatih University Medical School Laboratory use was filled in as required. We discussed our project with Prof. Dr. Mehmet Gunduz. Safety and security issues are found sufficient enough that no change is considered as necessary. (Further questions can be directed to Prof. Gunduz, tel: +90-312-203 5103, mgunduz@fatih.edu.tr).

c.Will / did you receive any biosafety and/or lab training before beginning your project? If so, describe this training.

Yes, we did. Our advisor provided us biosafety and lab training before starting of our project. In the training, general safety rules of laboratory use, prevention from hazardous chemicals and solutions as well as emergency intervention in case of accident were included.  

d. Does your country have national biosafety regulations or guidelines? If so, provide a link to them online if possible.

Yes, Turkey has national biosafety regulations and the link is given as (http://www.tbbdm.gov.tr/Home/BioSafetyCouncilHome/BioSafetyCouncilHomeChoose.aspx).

4. OPTIONAL QUESTION: Do you have other ideas on how to deal with safety or security issues that could be useful for future iGEM competitions? How could parts, devices and systems be made even safer through biosafety engineering?

First, IGEM committee may prepare a lab safety acknowledgement form like OSHA form. This form can be required to be filled in by all members. This will allow us to confirm whether members of the teams are informed about safety issues.
Second, a new prize related with security and safety issues may take place in the judging procedure separated from “Best Human Practices” prize. Existence of “Best Safety Part” or “Best Safety Application” can emphasize the importance of the safety issues and inspire iGEM teams to work on these issues and may give a chance to develop new ideas, to interrogate current safety and security applications in order to improve them.
Third, iGEM committee may organize a webinar to inform the participants about safety and security in labs. Webinar can be done at the beginning of the experiments. Mentor scientists may talk about their experiences and mention some tricks about safety issues.