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From 2011.igem.org

RecA Project Sensor Project Reporter Project

The Reporter

Our reporter system will use a modified version of Imperial College London iGEM 2010 fast reporter system. Imperial’s quick acting response module used the transcription of TEV protease to cleave the bond between the GFP, C23O fusion protein. This unbound form of C230 could then form a tetramer and convert the colorless substrate catechol into a yellow product.

Using the Freiburg BioBricking Assembly technique, we plan to produce a miniature catalog of fast acting reporters while reducing the leakiness of Imperial’s construct. To achieve the latter, we plan to link a second GFP to the C-terminus of C230; this new linkage would be cleavable by RecA.