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Revision as of 06:59, 28 September 2011 (view source) Ajb5663 (Talk | contribs) (Created page with "==Tuesday, July 26== Clone tev protease (K316017) into K3 ==Wednesday, July 27== All colonies from July 26 clone=red, retry clone tev protease into K3 Clone XylE and GFP into K3...") ← Older edit		Latest revision as of 07:23, 28 September 2011 (view source) Ajb5663 (Talk | contribs)
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		+	==Sunday, July 24==
		+	No colonies grew for Imp linker+KylE
		+
		+	Colony PCR small linker+XylE
		+
		+	Clone LacZα and GusA into K3 with new primers
		+	==Monday, July 25==
		+	tev protease mutagenesis
		+
		+	clone GusA and LacZα into K3
	==Tuesday, July 26==		==Tuesday, July 26==
-	Clone tev protease (K316017) into K3	+	Place linkers in front of XylE
-	==Wednesday, July 27==	+
-	All colonies from July 26 clone=red, retry clone tev protease into K3	+
-	Clone XylE and GFP into K3	+	Colony PCR GusA and LacZα clones
-	Prepare cultures of K316009 for catechol assay	+	Results: looked good on gel, grown up in culture
		+	==Wednesday, July 27==
		+	Miniprep and sequence GusA and LacZα clones
		+
		+	Results: Failure
	==Thursday, July 28==		==Thursday, July 28==
-	Colony PCR GFP+K3, XylE+K3, RecA+K3 and tev protease+K3	+	Clone GusA and LacZα into K3
-	Results: GFP+K3 and tev protease+K3 failed, all else worked	+
-	Test K316009 with catechol assay	+	Results from 7/22 assemblies:
-	Results: no difference between induced and uninduced cultures	+
		+	Only Pr-RBS-XylE-small linker-tev cleavage site-GFP worked
	==Friday, July 29==		==Friday, July 29==
-	clone tev protease into K3	+	Pr-RBS-XylE-linkers'''+'''cleavage sites+GFP
		+
		+	Colony PCR of GusA and LacZα clones
-	J23100+RBS34	+	Results: failure
-	GFP+tev and cI cleavage sites	+
	==Saturday, July 30==		==Saturday, July 30==
-	Colony PCR of J23100+RBS34, GFP+tev cleavage site, GFP+cI cleavage site	+	Colony PCR of 7/29 assemblies
-	Results: all failed	+
-	XylE+3 linkers	+	Results: failure
-	LacZα+linkers	+
	[[Team:Penn_State/Lab| Back to Wetlab]]		[[Team:Penn_State/Lab| Back to Wetlab]]

---

Latest revision as of 07:23, 28 September 2011

Contents 1 Sunday, July 24 2 Monday, July 25 3 Tuesday, July 26 4 Wednesday, July 27 5 Thursday, July 28 6 Friday, July 29 7 Saturday, July 30

Sunday, July 24

No colonies grew for Imp linker+KylE

Colony PCR small linker+XylE

Clone LacZα and GusA into K3 with new primers

Monday, July 25

tev protease mutagenesis

clone GusA and LacZα into K3

Tuesday, July 26

Place linkers in front of XylE

Colony PCR GusA and LacZα clones

Results: looked good on gel, grown up in culture

Wednesday, July 27

Miniprep and sequence GusA and LacZα clones

Results: Failure

Thursday, July 28

Clone GusA and LacZα into K3

Results from 7/22 assemblies:

Only Pr-RBS-XylE-small linker-tev cleavage site-GFP worked

Friday, July 29

Pr-RBS-XylE-linkers+cleavage sites+GFP

Colony PCR of GusA and LacZα clones

Results: failure

Saturday, July 30

Colony PCR of 7/29 assemblies

Results: failure

Back to Wetlab