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NZY+ Recipe
From 2011.igem.org
(Difference between revisions)
(Created page with "[https://2011.igem.org/Team:Panama/Protocols/Wetlab '''Back''']") |
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| + | NZY+ Broth (per Liter) | ||
| + | |||
| + | 10 g of NZ amine (casein hydrolysate) | ||
| + | |||
| + | 5 g of yeast extract | ||
| + | |||
| + | 5 g of NaCl | ||
| + | |||
| + | Add deionized H2O to a final volume of 1 liter. Adjust to pH 7.5 using NaOH. | ||
| + | Autoclave. | ||
| + | |||
| + | Add the following filer-sterilized supplements prior to use: | ||
| + | |||
| + | 12.5 ml of 1 M MgCl2 | ||
| + | |||
| + | 12.5 ml of 1 M MgSO4 | ||
| + | |||
| + | 20 ml of 20% (w/v) glucose (or 10 ml of 2 M glucose) | ||
| + | |||
[https://2011.igem.org/Team:Panama/Protocols/Wetlab '''Back'''] | [https://2011.igem.org/Team:Panama/Protocols/Wetlab '''Back'''] | ||
Latest revision as of 01:44, 29 September 2011
NZY+ Broth (per Liter)
10 g of NZ amine (casein hydrolysate)
5 g of yeast extract
5 g of NaCl
Add deionized H2O to a final volume of 1 liter. Adjust to pH 7.5 using NaOH. Autoclave.
Add the following filer-sterilized supplements prior to use:
12.5 ml of 1 M MgCl2
12.5 ml of 1 M MgSO4
20 ml of 20% (w/v) glucose (or 10 ml of 2 M glucose)
