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Mutagenesis PCR
From 2011.igem.org
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'''3.''' 5' - CTCGACCGAACACCTACAGGGCGACTTCTAC - 3' | '''3.''' 5' - CTCGACCGAACACCTACAGGGCGACTTCTAC - 3' | ||
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| + | [https://2011.igem.org/Team:Panama/Protocols/Wetlab '''Back'''] | ||
Latest revision as of 01:25, 29 September 2011
Mutagenesis PCR was done using the protocol established by agilent's Quiklightning Multi-site directed mutagenesis kit.
Our Mutagenesis Primers were:
1. 5' - CGAATCACGTGCTACAGCGCGCCTACG - 3'
2. 5' - ACCTGCCGCACTGTAGTTTCTCGCGGG - 3'
3. 5' - CTCGACCGAACACCTACAGGGCGACTTCTAC - 3'
