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Mutagenesis PCR
From 2011.igem.org
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| - | Mutagenesis PCR was done using the protocol established by agilent's [http://www.genomics.agilent.com/files/Manual/210514.pdf ''' | + | Mutagenesis PCR was done using the protocol established by agilent's [http://www.genomics.agilent.com/files/Manual/210514.pdf '''Quiklightning Multi-site directed mutagenesis kit.'''] |
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| + | '''Our Mutagenesis Primers were:''' | ||
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| + | '''1.''' 5' - CGAATCACGTGCTACAGCGCGCCTACG - 3' | ||
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| + | '''2.''' 5' - ACCTGCCGCACTGTAGTTTCTCGCGGG - 3' | ||
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| + | '''3.''' 5' - CTCGACCGAACACCTACAGGGCGACTTCTAC - 3' | ||
Revision as of 01:12, 29 September 2011
Mutagenesis PCR was done using the protocol established by agilent's Quiklightning Multi-site directed mutagenesis kit.
Our Mutagenesis Primers were:
1. 5' - CGAATCACGTGCTACAGCGCGCCTACG - 3'
2. 5' - ACCTGCCGCACTGTAGTTTCTCGCGGG - 3'
3. 5' - CTCGACCGAACACCTACAGGGCGACTTCTAC - 3'
