"
Copenhagen/31 August 2011
From 2011.igem.org
(Difference between revisions)
(Created page with "==Wednesday== The plates with User2C9 had no colonies. The human CYPs are therefore to no use and are definitively dropped. '''Labwork''' * Grow cells: UserB1 and Control Bl21...") |
(→Wednesday) |
||
| Line 7: | Line 7: | ||
* Grow cells: UserB1 and Control Bl21, Took SDS sample by 0 h. | * Grow cells: UserB1 and Control Bl21, Took SDS sample by 0 h. | ||
| - | * Made Gel, ran gel with PCR products from yesterday: A2 in new expression vector (1-8) and A2 in old expression vector (1-8). Positive result for A2 new | + | * Made Gel, ran gel with PCR products from yesterday: A2 in new expression vector (1-8) and A2 in old expression vector (1-8). Positive result for A2 new 4,5,7,8 and A2 old 2,4,6,7. |
* Miniprep on the O/N which showed a positive result on the gel. | * Miniprep on the O/N which showed a positive result on the gel. | ||
| Line 14: | Line 14: | ||
* Make O/N of UserB1 to sequencing tomorrow. | * Make O/N of UserB1 to sequencing tomorrow. | ||
| + | |||
Back to [[Team:Copenhagen/Notebook|notebook]] | Back to [[Team:Copenhagen/Notebook|notebook]] | ||
Latest revision as of 15:54, 31 August 2011
Wednesday
The plates with User2C9 had no colonies. The human CYPs are therefore to no use and are definitively dropped.
Labwork
- Grow cells: UserB1 and Control Bl21, Took SDS sample by 0 h.
- Made Gel, ran gel with PCR products from yesterday: A2 in new expression vector (1-8) and A2 in old expression vector (1-8). Positive result for A2 new 4,5,7,8 and A2 old 2,4,6,7.
- Miniprep on the O/N which showed a positive result on the gel.
- Transformation of positive A2s into Bl21 cells.
- Make O/N of UserB1 to sequencing tomorrow.
Back to notebook
