Copenhagen/28 June 2011

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Revision as of 09:40, 28 June 2011

Thuesday

Status

It seems that something went worng with our mutants from last week since we weren't able to transform E.coli with them - even though we used a larger amount of plasmid. So now we put all our faith in the mutants we made yesterday.

Lab work

  • Finished the mutation: Digested the parental plasmid
  • Transformed E.coli with our new mutant plasmids. Protocol Transformation
  • Took one colony out of our XL1-Blue transformed with biobrick no. BBa_J04500 and placed it in an overnight culture.