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From 2011.igem.org

Friday

Deadline for preliminary project description and safety page

Lab Work

A1

• We purified A1 with Mini prep from 3 different colonies.
• We analyzed A1 cut with Pst1 (which cut at the restrictionsite we want to remove)on an agarose gel. The results looked good and we sent them to be sequenced.

A2

• Double digestion of A1 using Kenneths approach.
• The plasmid was purified with gel purification

B1

We run a PCR to confirm that colony contains CYP79B1.

We plate the colony once again on agarplates with chloramphenicol hoping to collect a lot of cells after the weekend:)

Other work

Team building and listening to reggae.

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