Copenhagen/15 August 2011

From 2011.igem.org

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* Miniprep on the A1 and A2 in pSB1C3, so ready to send to iGEM after sequencing
* Miniprep on the A1 and A2 in pSB1C3, so ready to send to iGEM after sequencing
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* O/N for membrane preparation on A1
+
* O/N for Expression & Purification in BL21 on A1
* Analyzed the SDS-page from friday
* Analyzed the SDS-page from friday
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* Made TB media for membrane preparation
+
* Made TB media for Expression & Purification in BL21
* Try the USER-assembly again
* Try the USER-assembly again

Latest revision as of 13:02, 15 August 2011

Monday

Lab work

  • TLC on A2 (sub=phenylalanin) and A1 (sub=Tyrosin and a control with oxime) again
  • Amp plates
  • Miniprep on the A1 and A2 in pSB1C3, so ready to send to iGEM after sequencing
  • O/N for Expression & Purification in BL21 on A1
  • Analyzed the SDS-page from friday
  • Made TB media for Expression & Purification in BL21
  • Try the USER-assembly again
  • Autoclaved GMO-waste


Other work

  • Started on the poster for the Jamboree
  • Made an illustration of the expression of human CYP p450
  • Eat nice cake because Casper had birthday yesterday - congratulations to him!:)


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