Copenhagen/12 August 2011

From 2011.igem.org

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(Friday)
(Friday)
 
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* Took the last A2 sample for the SDS-PAGE assey.
* Took the last A2 sample for the SDS-PAGE assey.
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* Membrane preparation on A2  
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* Run a [[Team:Copenhagen/Protocol#SDS-PAGE|SDS-PAGE]] on A2.
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* [[Team:Copenhagen/Protocol#Expression & Purification in BL21|Membrane preparation]] on A2.
* Colony PCR on A1 and A2 in pSB1C3 follwed by gel electroforesis. The biobricks were ligated into the plasmid:)
* Colony PCR on A1 and A2 in pSB1C3 follwed by gel electroforesis. The biobricks were ligated into the plasmid:)
   
   
[[image:gel1208.jpg|400px|left]]
[[image:gel1208.jpg|400px|left]]
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From left to right: marker, A1 5-1 and A2 8-1)
From left to right: marker, A1 5-1 and A2 8-1)
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'''Other Work'''
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* Casper has written two verses for the Cyoerman song

Latest revision as of 14:09, 15 August 2011

Friday

Lab Work

  • Took the last A2 sample for the SDS-PAGE assey.
  • Colony PCR on A1 and A2 in pSB1C3 follwed by gel electroforesis. The biobricks were ligated into the plasmid:)
Gel1208.jpg














From left to right: marker, A1 5-1 and A2 8-1)

Other Work

  • Casper has written two verses for the Cyoerman song




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