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Copenhagen/11 July 2011
From 2011.igem.org
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*2 overnight cultures of A2 was put in a shaking cabinet in 37 degrees | *2 overnight cultures of A2 was put in a shaking cabinet in 37 degrees | ||
| - | *Biobrick A1 og A2 was [[Team:Copenhagen/Protocol#Mutations|mutated]] (again with template amounts 10,25 and 50 nm) and XL1-Blue competent cells were [[Team:Copenhagen/Protocol# | + | *Biobrick A1 og A2 was [[Team:Copenhagen/Protocol#Mutations|mutated]] (again with template amounts 10,25 and 50 nm) and XL1-Blue competent cells were [[Team:Copenhagen/Protocol#Transformation|transformed]] |
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| + | * We extended our cyp B1 with prefix and suffix by a PCR [[Team:Copenhagen/Protocol#PCR Reaction|PCR approach]]. | ||
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| + | The DNA obtained was purified by using a PCR purification kit, followed by gel purification and then [[Team:Copenhagen/Protocol#Gel Extraction Protocol|extracted]] the DNA from the gel. | ||
| + | The B1 DNA is now ready to be transfected into expression vectors. | ||
Revision as of 14:59, 11 July 2011
Monday
Lab Work
- 2 overnight cultures of A2 was put in a shaking cabinet in 37 degrees
- Biobrick A1 og A2 was mutated (again with template amounts 10,25 and 50 nm) and XL1-Blue competent cells were transformed
- We extended our cyp B1 with prefix and suffix by a PCR PCR approach.
The DNA obtained was purified by using a PCR purification kit, followed by gel purification and then extracted the DNA from the gel. The B1 DNA is now ready to be transfected into expression vectors.
