Copenhagen/10 August 2011

From 2011.igem.org

(Difference between revisions)
(Created page with "===Wednesday=== '''Lab Work'''")
(Wednesday)
Line 1: Line 1:
===Wednesday===
===Wednesday===
 +
 +
The single A2 colony plated yesterday in pSB1C3 were all red today = no insert!
 +
 +
Transformation of B1 and A2 in pSB1C3 from yesterday. B1 had lot of colonies but red = no insert. A2 had a lot of colonies as well, but some were white! 8 colonies were picked out and replated and an O/N culture made.
 +
'''Lab Work'''
'''Lab Work'''
 +
 +
* 8 A2 colonies (pSB1C3) were picked out and replated and an O/N culture made.
 +
 +
* Membrane preparation of O/N culture of IPTG induced A1 expressing cells.
 +
 +
* Measured absorption spectra on proteins from the membrane fraction. Despite helt from Johan, Thomas, Peter and Birger, we did not succeed to confirm active CYP79A1. There might be a problem with the CO container or the dithionite used (either could be oxidated from the air, and therefore useless).   
 +
 +
 +
'''Other Work'''
 +
 +
* Had a very nice meeting with Jaide from the DTU-2 Team. She gave us DNA pieces to do User Cloning on CYP79A2 and B1.

Revision as of 16:16, 10 August 2011

Wednesday

The single A2 colony plated yesterday in pSB1C3 were all red today = no insert!

Transformation of B1 and A2 in pSB1C3 from yesterday. B1 had lot of colonies but red = no insert. A2 had a lot of colonies as well, but some were white! 8 colonies were picked out and replated and an O/N culture made.


Lab Work

  • 8 A2 colonies (pSB1C3) were picked out and replated and an O/N culture made.
  • Membrane preparation of O/N culture of IPTG induced A1 expressing cells.
  • Measured absorption spectra on proteins from the membrane fraction. Despite helt from Johan, Thomas, Peter and Birger, we did not succeed to confirm active CYP79A1. There might be a problem with the CO container or the dithionite used (either could be oxidated from the air, and therefore useless).


Other Work

  • Had a very nice meeting with Jaide from the DTU-2 Team. She gave us DNA pieces to do User Cloning on CYP79A2 and B1.