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Agar plates
From 2011.igem.org
(Difference between revisions)
| Line 1: | Line 1: | ||
| - | Materials: | + | '''Materials:''' |
LB broth | LB broth | ||
| Line 7: | Line 7: | ||
Antibiotic | Antibiotic | ||
| - | Procedure: | + | '''Procedure:''' |
1. mix 20 grams of agar powder for every 1000ml of LB broth being used. | 1. mix 20 grams of agar powder for every 1000ml of LB broth being used. | ||
Latest revision as of 03:03, 29 September 2011
Materials:
LB broth
Agar powder
Antibiotic
Procedure:
1. mix 20 grams of agar powder for every 1000ml of LB broth being used.
2. autoclave
3. wait till temperature has gone down to a point were it wont burn to touch and add the appropriate antibiotic at the desired concentration.
4. serve 25ml of agar+LB solution per petri dish.
