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Agar plates
From 2011.igem.org
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| + | Materials: | ||
| + | |||
| + | LB broth | ||
| + | |||
| + | Agar powder | ||
| + | |||
| + | Antibiotic | ||
| + | |||
| + | Procedure: | ||
| + | |||
| + | 1. mix 20 grams of agar powder for every 1000ml of LB broth being used. | ||
| + | |||
| + | 2. autoclave | ||
| + | |||
| + | 3. wait till temperature has gone down to a point were it wont burn to touch and add the appropriate antibiotic at the desired concentration. | ||
| + | |||
| + | 4. serve 25ml of agar+LB solution per petri dish. | ||
| + | |||
[https://2011.igem.org/Team:Panama/Protocols/Wetlab '''Back'''] | [https://2011.igem.org/Team:Panama/Protocols/Wetlab '''Back'''] | ||
Revision as of 03:03, 29 September 2011
Materials:
LB broth
Agar powder
Antibiotic
Procedure:
1. mix 20 grams of agar powder for every 1000ml of LB broth being used.
2. autoclave
3. wait till temperature has gone down to a point were it wont burn to touch and add the appropriate antibiotic at the desired concentration.
4. serve 25ml of agar+LB solution per petri dish.
