Agar plates

From 2011.igem.org

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'''Materials:'''
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LB broth
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Agar powder
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Antibiotic
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'''Procedure:'''
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1. mix 20 grams of agar powder for every 1000ml of LB broth being used.
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2. autoclave
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3. wait till temperature has gone down to a point were it wont burn to touch and add the appropriate antibiotic at the desired concentration.
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4. serve 25ml of agar+LB solution per petri dish.
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[https://2011.igem.org/Team:Panama/Protocols/Wetlab '''Back''']
[https://2011.igem.org/Team:Panama/Protocols/Wetlab '''Back''']

Latest revision as of 03:03, 29 September 2011

Materials:

LB broth

Agar powder

Antibiotic

Procedure:

1. mix 20 grams of agar powder for every 1000ml of LB broth being used.

2. autoclave

3. wait till temperature has gone down to a point were it wont burn to touch and add the appropriate antibiotic at the desired concentration.

4. serve 25ml of agar+LB solution per petri dish.

Back