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From 2011.igem.org

Revision as of 15:10, 24 September 2011 (view source) Sallen (Talk | contribs) ← Older edit		Latest revision as of 15:10, 24 September 2011 (view source) Sallen (Talk | contribs)
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-	Created TENS
-	:4.5 mL TE and
-	::250uL 10% SDS
-	::250uL 2N NaOH
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-
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-	:-Spin 4 tubes (1.5mL) 30"
-	:-Pour off sol'n & resuspend
-	:-Add
-	::300uL TENS:    mix
-	::150uL NaOH:    mix
-	:-Spin 2.5 min.
-	:-Transfer supernatant to clean tube and add 1mL of EtOH
-	:-Mix and pellet DNA by centrifugation for 2-5 min
-	:-Wash pellet with 70% ethanol and allow pellet to dry
-	:-Resuspend the pellet in 30λ of the TE
-	:-Digest 5-10λ
-
	<gallery widths=910px heights=800px>		<gallery widths=910px heights=800px>
	File:May26June6GDS.jpg		File:May26June6GDS.jpg
	</gallery>		</gallery>

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Latest revision as of 15:10, 24 September 2011