26 (May 26, 2011 GDS)

From 2011.igem.org

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Created TENS
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<gallery widths=910px heights=800px>
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:4.5 mL TE and
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File:May26June6GDS.jpg
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::250uL 10% SDS
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</gallery>
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::250uL 2N NaOH
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:-Spin 4 tubes (1.5mL) 30"
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:-Pour off sol'n & resuspend
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:-Add
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::300uL TENS:    mix
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::150uL NaOH:    mix
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:-Spin 2.5 min.
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:-Transfer supernatant to clean tube and add 1mL of EtOH
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:-Mix and pellet DNA by centrifugation for 2-5 min
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:-Wash pellet with 70% ethanol and allow pellet to dry
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:-Resuspend the pellet in 30&lambda; of the TE
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:-Digest 5-10&lambda;
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Latest revision as of 15:10, 24 September 2011