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From 2011.igem.org

Parth--made fresh TENS

4.5 TE

250λ 10% SDS

250λ 2M NaOH

Spin 1.5 mL (2 tubes) 30"

Pour off sol'n + resuspend

Add: 300λ TENS mix

150λ NaOAc mix

Spin 2.5'

Sup to new tube

Add 1 mL ETOH - mix well

Spin 3' ________

Wash 70% ETOH

Minipreps (2 each) for:

pNICE SA-15λ TE

J024430 GE-15λ TE

Trans002-5, AB-Resp 30λ

Trans003-W1 SD, SA-