Team:Korea U Seoul/Project

From 2011.igem.org

Revision as of 12:47, 27 August 2011 by Ssyy90 (Talk | contribs)
You can write a background of your team here. Give us a background of your team, the members, etc. Or tell us more about something of your choosing.
Korea U Seoul logo.png

Tell us more about your project. Give us background. Use this is the abstract of your project. Be descriptive but concise (1-2 paragraphs)

File:Korea U Seoul team.png
Your team picture
Team Example


Home Team Official Team Profile Project Parts Submitted to the Registry Modeling Notebook Safety Attributions



Contents

Overall project

The goal of our project is to produce alkane chains from glucose molecules. In nature, numerous biochemical pathways and enzymes exist, making life adoptable to even extreme conditions such as volcanic regions. We focused on biochemical pathways, enzymes of glucose metabolism and luminescene luciferase from Vibrio harveyi to achieve our goal. Based on glycolysis, pyruvate oxidation, enzymes coded in luciferase genes (lux operon) and FAC from cyanobacteria, glucose is turned into alkane chain of about 14 carbon atoms in length. Synthesized fuel is functionally identical to natural petroleum and can be used as bioenergy. Produced alkane chain is part of a carbon circulation cycle as it is synthesized from glucose, in vivo. The fuel is relatively environment-friendly, unlike ordinary petroleum which increases CO2 concentration in the atmosphere. Though the production of alkanes using bioblock could be not satisfied commercially, succeeding in the synthesis of alkane chains from glucose nevertheless will show another method of producing alternative energy source. Therefore, the success of this research will contribute to global effort in reducing atmospheric CO2 levels.

Project Details

We believe that there exists difficulty in producing Wax ester. Therefore, we read carefully several dissertations in order to find a compound that we can use as biofuel. One of our members came up with an idea after finding a picture(shown below) about a synthetic pathway in fluorescing bacteria.

frame less

According to the picture above, it is possible to turn Acyl-ACP into a C14 aldehyde. We can then deduce from such a result the possibility of producing a C14 alkane from glucose, utilizing microorganisms as a synthetic machinery.

By considering such possibilities, team members began searching for pathways that utilize two substances, which are: 1. luxAB removed luxCDE from fluorescing bacteria 2. cyanobacteria's aldehyde decarbonylase gene

Picture below depicts biofuel synthetic pathway.

thumb nail


Synthetic Fatty Acids Reduction Pathway

thumb nail



Cloning of target genes

thumb nail

Results