Team:Paris Liliane Bettencourt/Notebook/2011/08/03

From 2011.igem.org

Kevin

Culture of double transformed cells (suite 2)

Diluted at 1/100 => expected to be between OD 300 and 600

• 3 Tubes pSG20 at 37°C
• 3 Tubes pFX234 at 37°C

• 3 Tubes pSG20 only at 30°C
• 3 Tubes FX234 only at 30°C
• 3 Tubes pSG20/pDAG464 at 30°C
• 3 Tubes pFX234/pDAG479 at 30°C

At OD 300-600, induction of fusion protein with arabinose 0%, 0,1 % and 0,2% for 1h30.

Microscopy (suite 2)