"

From 2011.igem.org

Contents 1 green light receptor 1.1 NAME OF YOUR EXPERIMENT 2 blue light receptor 2.1 NAME OF YOUR EXPERIMENT 3 red light receptor 3.1 3A-assembly: Digest 3.2 3A-assembly: Ligation and transformation 4 Lysis cassette 4.1 NAME OF YOUR EXPERIMENT 5 Precipitator 5.1 Test-digest

green light receptor

NAME OF YOUR EXPERIMENT

Investigators:NAME

blue light receptor

NAME OF YOUR EXPERIMENT

Investigators:NAME

red light receptor

3A-assembly: Digest

Investigators: Jakob

3A-assembly of S22 (ho1)+S21a (terminator) and S23 (pcyA)+S21a (terminator)

S21a:

• Enzyme 1: XbaI
• Enzyme 2: PstI

S22, S23

• Enzyme 1: EcoRI
• Enzyme 2: SpeI

Vector:

• Enzyme 1: EcoRI
• Enzyme 2: PstI
• Enzyme 3: DpnI

3A-assembly: Ligation and transformation

Investigators: Julia

Ligation of S22 (ho1)+S21a (terminator) and S23 (pcyA)+S21a (terminator) in vector pSB1T3.

L23: Ligation of S23+S21a in pSB1T3 L22: Ligation of S22+S21a in pSB1T3

Plasmids were transformed in cells and plated out.

Lysis cassette

NAME OF YOUR EXPERIMENT

Investigators:NAME

Precipitator

Test-digest

Investigators: Rüdiger

Testdigest of the minipreps, Sophie did (june, 13).

• Enzyme 1: PstI
• ENzyme 2: EcoRI

Image of different samples from miniprep.