Team:ETH Zurich/Achievements/Data page

From 2011.igem.org

Revision as of 22:16, 18 October 2011 by Sabineoesterle (Talk | contribs)

Menu image preload Menu image preload Menu image preload Menu image preload Menu image preload Menu image preload


Data page
How Our System Works New Parts Characterization
SmoColi cells are engineered to sense toxic substances found in cigarette smoke. They are immobilized in a microfluidic channel, in which a concentration gradient of the toxic substance is established. The sensor is linked to a band-pass filter that leads to input-concentration-dependent GFP expression. Continuous increase of the input concentration and its detection, therefore, establishes a moving fluorescent band in the channel. Finally, if the input concentration exceeds a certain threshold, cells produce RFP and the device turns red.

How Our System Works

Figure 1: Molecular mechanism of SmoColi with xylene sensor, depending on the xylene input, either a spatially limited GFP or global RFP signal is produced in the channel.

Data For Our Favorite New Parts

  1. [http://partsregistry.org/Part:BBa_K625000 MainPage]-LacIM1, BBa_K625000; E. coli codon-modified LacI designed to avoid recombination with the wild type LacI in a system where both variants are present, LacIM1 binds to Plac and inhibits transcription. (Characterization)
  2. [http://partsregistry.org/Part:BBa_K625005 MainPage]-Psb6A5, BBa_K625005; improved Plasmid pSB6A1 (ETHZ, 2007): transcriptional terminators added. (Characterization)
  3. [http://partsregistry.org/Part:BBa_K625003 MainPage]-PU promoter, BBa_K625003; Promoter regulated by the transcriptional activator XylR (shortened version) (Characterization)

We've also characterized the following parts

  1. [http://partsregistry.org/Part:BBa_K625001 MainPage]- PTet - LacIM1 - Termination, BBa_K625001 (Characterization)
  2. [http://partsregistry.org/Part:BBa_K625002 MainPage]-PU promoter, BBa_K625002; Promoter regulated by the transcriptional activator XylR (long version with stop codon) (Characterization)
Retrieved from "http://2011.igem.org/Team:ETH_Zurich/Achievements/Data_page"