Team:NYMU-Taipei/results/immunological-solution1

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Construction & Parts



Our MinC construct and Inv&LLO construct


AMB-1 minC NYMU.jpg
Fig. Design of MinC Construct on AMB-1
Backbone Plasmid: pYMB
Fig. Design of Inv&LLO Construct on AMB-1
Backbone Plasmid: pRKM415

tetR+RBS+GFP



measurements of construct tetR+RBS+GFP under fluorescence microscope


30.JPG 31.JPG 32.JPG


These are the measurements of our construct tetR+RBS+GFP under fluorescence microscope. The left graph is from the control group of E. coli strain DH5a. The middle is tetR+RBS+GFP in plasmid c3 in E. coli, while the right is from a plate without any bacilli. All graphs are measured in fixed exposure time. Construct of tetR+RBS+GFP which emits light is thought to be a leak phenomenon of plasmid, for RE digest has proven the sequence length is correct, and further sequencing work is under operation.



GFP measurements under different excitation wavelengths

33.JPG


The expression of GFP is measured precisely under excitation wavelength from 475nm to 501nm, given the absorption wavelength 511nm. In our measurement, wavelength over 493nm yields high background, resulting in values of control group surging far beyond normal condition. Thus, 493nm is suggested as the best excitation wavelength, as it presents the highest emission light and the lowest of the control group.