Team:SJTU-BioX-Shanghai/Project/Subproject1/Results

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    •



    Rare-Codon Switch

    Results

    Action: Combinations of Modulators and Reporters

    Select a Modulator and a Reporter, then click 11SJTU arrow.jpg to see results!


    tRNA Modulator System

    • System Analysis

    tRNA Modulator + Reporter for Qualitative Analysis

    Action: when rare tRNAArg is over expressed, the amount of tRNAArg in the tRNA pool is largely increased, which facilitates ribosome to get through the tandem of AGG codons in the RFP's mRNA.

    tRNA Modulator + Reporter for Quantitative Analysis Action: when rare tRNAArg is over expressed, the amount of tRNAArg in the tRNA pool is largely increased, which facilitates ribosome to get through the tandem of AGG codons in the modified luciferase's mRNA.

    • Results Conclusion


    aaRS Modulator System

    Action: When TDRS is expressed under IPTG induction, it can charge tRNAAsp-AGG with Asp. tRNAAsp-AGG then recognizes the rare codon AGG on RFP's mRNA and add Asp to the polypeptide chain. RFP is expressed.


    Action: When TDRS is expressed under IPTG induction, it can charge tRNAAsp-AGG with Asp. tRNAAsp-AGG then recognizes the rare codon AGG on the modified luciferase's mRNA and add Asp to the polypeptide chain. Modified luciferase is expressed.

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