Team:USTC-China/Project/results

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Contents

Experimental Results of the Original Design

Verification of ΔcheZ strain (protocol)

    The size of colonies of E.coli strain RP1616 was much smaller than that E.coli steain RP437 under the same circumstance and after same period of incubation time(about 10h), and the result is shown in Figure1. In colony PCR using the primers of cheZ gene following , RP437 absolutely has much more outcomes than RP1616 and we conclude that RP1616 is actually a ΔcheZ strain.

Figure1.

Figure1.The result of colony PCR(From left to right, the first lane is the marker, the third and the forth lane is the PCR outcome of strain RP437 and strain RP1616, the sixth and the seventh lane is the PCR outcome of strain RP437 and strain RP1616.)

Verification of the function of the constructed riboswitch (protocol)

(A)0.3%agar with 0mM Thephylline

(A)0.3%agar with 0mM Thephylline

(B)0.3%agar with 0.25mM Thephylline

(B)0.3%agar with 0.25mM Thephylline

Figure1.

    From the results above we concluded that the constructed riboswitch functions well, especially on 0.3% Semisolid medium.

Verification of Toggle-switch (protocol)

                   Results14.jpg     Results15.jpg

Numbers of colonies with RFP: Numbers of colonies with GFP ≈ 8:25

Modulate Toggle-switches to produce more balanced ratio (protocol)

                        Results6.jpg     Results17.jpg

Numbers of colonies with RFP: Numbers of colonies with GFP ≈ 6:1

Test of Incorporated Riboswitch into one side of Toggle-switch (protocol)

                    Results18.jpg   Results9.jpg

Verification of the constructed system’s function (protocol)

Results110.jpg
  • 6 tubes of constructed monoclonal RP1616
Results111.jpg
  • Results of the sixth tube

                    Results12.jpg          Results113.jpg