jun 28-july 4
2011.6.28
cultivate the bacteria of cheZ deficiency(RP1616),and the Control group(RP437).
check the resistibility of RP1616 and RP437
result:both of the two groups are of none resistibility.
members:Siwei Luo, Fangzhou Zhao, Zhilin Chen...
2011.6.29
check the motility of RP1616 strain and RP437 strain
cultivate Top10 strain
result: the bacterial plaque of RP1616 is smaller than that of RP437, implying that RP1616 is of cheZ deficiency, causing the decreased motility of the bacteria.
6.30
prepare for the competent cell of Top10 strain cultivated on 6.29
extract the genome of Top10 strain and use it as complates to run PCR of CheZ
result: the concentration of PCR result is too low to continue the experiments.
7.1
conduct PCR of CheZ again
ligate the CheZ DNA segment to plasmid PSb1C3, and transform it into Top10 competent cells
result:as the picture shows.
7.2
pick up single colony from the Petri dish to reproduce, in which the competent cells are cultivated and use them colony PCR
result:The bacteria grow up well on dishes with chloromycetin resistency，verifying that the transformation is successfull
while the concentration of PCR product is pretty high,demonstrating that the plasmids indeed contains the sagment CheZ.
7.3
extract the plasmids from the reproduced bacteria and send it to check the base sequence
result: the concentration of the plasmids extracted is about 500ng/uL
7.4
extract the standard parts LuxPR and Terminator from plate sent by iGEM registry 2010 and put them into Top10 strain by transformation
(LuxPr: Plate 2,24C Terminator : Plate 1,6O)
result: the bacteria on the plate with Amp resistency grows well
july 5-july 11
7.5
pick up single colony from the Petri dish to reproduce in the liquid culture medium
7.6
extract plasmids containing LuxPR and Terminator and make enzyme digestion with EcoR1 and Spe1 in order to validate the existence of the two parts
recieve part Toggle Switch and rbs-Ci-ter from PKU and conduct transformation
result: Due to the short length of LuxPR and Terminator, the result of the enzyme digestion is hard to examine by electrophoresis.
7.7
result: there are a few colonies with part Toggle switch grown while no colony of rbs-Ci-ter appeared.
7.8
pick up single colony with part Toggle switch .
extract the standard parts LuxPR and Terminator from plate sent by iGEM registry 2011 and put them into Top10 strain by transformation
7.9
pick up single colony with LuxPR & terminator.
extract plasmids containing toggle switch to conduct PCR.
process PCR with plasmids aptamer-CheZ
result:as the picture shows.
the base sequence of CheZ extracted before is proved right.
7.10
ligate aptamer-CheZ with PSB1C3 plasmids
extract plasmids containing LuxPR and terminator
result:the concentractions of plasmids LuxPR and terminator are both 600ng/ul
7.11
transform PSB1C3 containing aptamer-CheZ into bacteria and cultivate.
july 12-july18
7.12
carry out Double digestion of toggle switch and pSB1c3 and ligate them together.
pick up single colony from bacteria cultivated yesterday.
result: Both the location and the brightness of the electrophoretic bands correspond with our expectation.
7.14
transform the part rbs-ci-ter sent from PKU again into bacteria.
check the colors of the colonies containing toggle switch.
result: the ratio of red vs green is 8:25, verifying the function of toggle switch.
7.15
pick up the single colony from the plate yesterday and ten hours later extract the plasmids containing Ci-terminator.
7.16
conduct PCR with the plasmids yesterday to reproduce rbs-ci-Term.
result:failure
7.18-7.21
ligate the standard part Terminator to the end of toggle switch
result: we conduct a enzyme digestion with EcoR1 and Pst1 to the final ligation product and the electrophoresis result shows that the ligation is successful.
Fangzhou Zhao
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Hi,everyone!I am Zhao Fangzhou, a sophomore student majoring in Life Science in USTC. Born in the autumn of 1991, I was lucky enough to enjoy a 21-year life filled with happiness and sadness. Shangrao, located in the northeast of Jiangxi Province, is the very place where I was brought up. This beautiful town, positioned in Southern China, with its natural scenery and cultural heritage, has been teaching me how to handle with my own life and with the whole world. Over the years before, I have developed interests in a variety of occupations, like reading books, playing computer games and various ball games. However, since the day I was admitted into USTC, I began to devote much of my time to studying, and correspondingly cut down the time spent on after-class activities, expecting that two years later, I can walk out of this university without any regret.
Shaohua Li
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Name: Shaohua LiO(∩_∩)O！
Email: lsh7763@mail.ustc.edu.cn
Education: School of Life Sciences, USTC
Major: Medicinal Biotechnology
Hobby: take trips
Motto:Eat well,sleep well. In the meantime ,play with Ecoli.↖(^ω^)↗
Mo Li
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Mo Li:Symbol of element 3, that is Li,Lithium./Symbol of element 42,Molybenum,that is Mo./What if they react with each other? /Bimetal?/Not excatly./It's Li Mo/That's me！motto: E.coli is my lovely pet!
Mengping Sun
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Hi, I'm Mengping Sun, an IGEMer of team USTC-China. This is my last year as an undergraduate student and I am pround of being an IGEM fan. IGEM not only marks a start for my reserch career but also is a good way to meet many friends from all over the world. As for me, I am a person who love sports, movies and chatting with others. Being an IGEMer is lucky for the world becomes smaller when we have one commom dream. And it's a great honor to fulfill it in MIT.
Yufeng Luo
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Hello, everyone, I’m Yufeng Luo, an undergraduate from USTC-China team. My major is Chemistry. I’m honored to join the IGEM2011 competition, the memory of which will never be forgotten in my life.During last seven months I’ve being studied and worked with our teammates. In our project, I take part our experiments in constructing our system. We spent lots of time together, it’s not happy all the time, but every minites is meaningful. As The Grand Final coming,we are confident to have a good result. Best wishes(∩_∩).
Ning Wang
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Yuntao Liu
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Dan Zhou
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Name:Dan Chou
Email: zhoudan@mail.ustc.edu.cn
Study field: Chemistry
Interests: Music&Movies
Research Interest: Biochemistry
I am the clerk and the manager of USTC-China 2011 iGEM team, and I am the secretary-general of 2011 iGEM China-meetup. I also participated in the experiment of constructing the prompter, aptamer, cheZ and GFP together. I feel this enjoyable all the time.
Linna An
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Hi,I'm Anlina,and my Chinese name is also Linna An.I am now a new sophomore in USTC,the department of Chemistry and Material Science.I designed the wiki,completed it with my friend Yuntao Liu,and did some experiments with other teammates.But the most valuable experience is that I participated a complete project,which will definately help with my career.Not everything is easy,but there is always others to help.Not every day is happy,yet all the difficulties made what we are.When there is will,there is way!
Junhui Peng
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Junhui Peng
Senior undergraduate in Lab of Computational Biology, School of Life Sciences, USTC.
I think, it must be very exciting to find and understand the principles of how macromolecules organize in universe. In iGEM lab and compubio lab, you can try to build your own principles for bacteria and for macromolecules. Badminton is my favorite sport, and Defense of the Ancients is my favorite computer game.
Yanyue Wang
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I am Wang Yanyue from Department of Material Science and Engineering. You can call me Era for short. In our team I am in charge of the Human Practice part and also make my contribution to performing some experiments. The reason why I joined the team was that biology is so mysterious and fancy for an outsider like me, and I wish one day I may be able to untangle some of the fascinating enigmas of living creatures.Well, it is a bliss that we are living in such a wonderful world with so many lovely people caring about each other. We are so young and have the hearts to live what seemed impossible and challenging. As long as we have enough courage and wit, we are the ones who can make a change!
Xiaolei Zhang
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My name is Xiaolei Zhang,an undergraduate of system biology in USTC. While I began to cast doubt on the my conviction of studying biology,synthetic biology with the iGEM came to touch me, fascinating me with its innovative application of engineering idea in biology.In retrospect,all the diverse elements in this iGEM trip make me to see biology and scientific research from a refreshing point of view.Anyway,whether our research is serious enough,I take it seriously and cherish this iGEM trip.
Yang Zhou
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Yanyue Wang
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I am Wang Yanyue from Department of Material Science and Engineering. You can call me Era for short. In our team I am in charge of the Human Practice part and also make my contribution to performing some experiments. The reason why I joined the team was that biology is so mysterious and fancy for an outsider like me, and I wish one day I may be able to untangle some of the fascinating enigmas of living creatures.
Well, it is a bliss that we are living in such a wonderful world with so many lovely people caring about each other. We are so young and have the hearts to live what seemed impossible and challenging. As long as we have enough courage and wit, we are the ones who can make a change!
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