Team:Calgary/Notebook/Protocols/Process7

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Plasmid Extraction

This protocol will purify plasmids from bacteria, though identification of plasmids will need to be confirmed by PCR.


Reagents

  1. HEPES (N-[2-hydroxyethyl] piperazine N'[2-ethane-sulfonic acid])
  2. Sorbitol
  3. Percoll
  4. PEG 6000
  5. Ficoll
  6. Bovine serum albumin (BSA)
  7. Isoascorbic acid
  8. Sodium pyrophosphate
  9. Glutathione
  10. MgCl2.6H2O
  11. MnCl2
  12. Sodium-EDTA
Equipment