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From 2011.igem.org

Fixation Results

Aims

-To find a suitable material for encapsulation

-To build a construct that will appropriately express this material within a biofilm

-To transform this construct into the chassis and express it

-To characterise the expression and its effects towards fixation and encapsulation of the biofilm

Methods

We found our encapsulating material in Capsular Colanic Acid (Chao & Zhang, 2011). For simplicity it was decided to use an existing colanic acid biobrick, from the Imperial College iGEM 2009 Team.

The parts of the colanic acid system that were required to induce overexpression and export were selected. Top10 cells were transformed with the biobricks and the DNA was extracted to begin building a construct. We began attaching Ribosome Binding Sites and terminators to the biobricks we required, but did not finish the entire construct.

References

Chao, Y. & Zhang, T., 2011. Probing Roles of Lipopolysaccharide, Type 1 Fimbria, and Colanic Acid in the Attachment of Escherichia coli Strains on Inert Surfaces.Langmuir. 2011 Sep 20;27(18):11545-53