Team:Paris Liliane Bettencourt/Notebook/2011/09/04/

From 2011.igem.org

Revision as of 13:59, 4 September 2011 by Kyauy (Talk | contribs)

Contents

Kevin

Biobrick the YFP:tetR fusion protein

Let's start again with good primers to biobrick the protein.

PCR

3 Temperatures of primer annealing: 85 - 58 - 48 °C
Primers : pFX234 FW - pFX234 Reverse CP

Following steps

PCR Purification, digestion in ES, PCR Purification again, Ligation

To be continued

Transformation, sequencing and glycerol.