Team:Paris Liliane Bettencourt/Notebook/2011/09/04

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Danyel

Overview

The constructs involved in the tRNA project so far:

  • tRNA
    • S38: in pSB1C3
  • tRNA_TT
    • S58: in pSB1C3
  • Pveg_spoVG_tRNA
    • S38 in S24 --- [in progress]
  • Pveg_spoVG_tRNA_TT
    • S81 : this contruct in pSB1AK3 (ampR)
    • S92 : this contruct in pHM3 (ampR/tetR) --- sequencing required and transformation in subtilis attempted by Adrien.
  • pHyperspank_tRNA
    • S38 in S82 --- [in progress]
  • pHyperspank_tRNA_TT
    • S58 in S82 --- [in progress]
  • pT7_RBS_GFP_TT  :
    • S79 : this construct in ampR plasmid (pSB1AK3?).
    • functionality has been tested through transformation in BL21 and microscopy. (cf.27/08/11)