Team:Uppsala-Sweden/Notebook
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Uppsala University.
Welcome to Uppsala-SwedeniEM '2011
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Notebook
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Week 1
This week was dedicated to make buffers such as CCMB80 and SOC, SOB and LB medium (See protocol SOB- medium, LB medium and Competent cell preparation final). We also prepared selective agar plates with ampicillin, chloramphenicol and kanamycin (See agarplate preparation). Finally we prepared competent TOP10 cells and froze them in -80°C (See Competent cell preparation final).
Week 2
2011-06-27
- This week we started with testing the competence in our cells. For this transformation we used one positive control (plasmid pUC19 from New England Biolabs) as well as a negative control without plasmid. For the procedure we followed the protocol for transforming TOP10 competent cells. The result was good, we measured a competence efficiency of 1.7 * 108 transformants /ug DNA.
- Started overnight cultures of E coli carrying the plasmids pGEM11- amilGFP (green output) and pGEM14- amilCP (blue output). For this procedure we followed the protocol Overnight culture and glycerol stock. The strains carrying the amilGFP and amilCP plasmids were provided by J.F Miller, UCLA.
2011-06-28
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2011-06-29
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2011-06-30
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2011-07-01
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Week 3
2011-07-04
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2011-07-05
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2011-07-06
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2011-07-07
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2011-07-08
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Week 4
2011-07-11
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2011-07-12
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2011-07-13
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2011-07-14
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2011-07-15
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Week 5
2011-07-18
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2011-07-19
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2011-07-20
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2011-07-21
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2011-07-22