Team:Lyon-INSA-ENS/Realisation/Week7Fr

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Semaine 7


From Monday the 25th of July to Friday the 29th of July 2011







Monday


Miniprep of some other parts from the transformed bacteria. Digestion and electrophoresis to control the presence of the right insert.

Miniprep of bacteria with mutated part CsgAB. Digestion to check restriction profile (removal of intern PstI site).



Tuesday



Send of plasmid with the right profile for part CsgAB to sequencing. Extraction of new MC4100 strain's DNA. PCR with fresh DNA and Taq Phusion.





Wednesday


PCR did not work.



Jeudi





Vendredi

La PCR a finalement marché avec la PHU 9104.








ENS assystem Biomérieux INSA INSA