Team:Grinnell/Notebook/Gels

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PCR test of transformation products of insertion of gel extracted DNA into plasmid containing another gene. Lane 1: ladder; lanes 2-4: insertion of <i>rsaA</i> C-terminal into plasmid containing <i>esp</i>; lanes 5-7: insertion of <i>esp</i> into plasmid containing <i>rsaA</i> C-terminal.  Smearing of bands is inconclusive and suggests that there may be no DNA (colonies are contaminants) or that freeze-thaw did not succeed in providing sufficient template DNA for PCR.
PCR test of transformation products of insertion of gel extracted DNA into plasmid containing another gene. Lane 1: ladder; lanes 2-4: insertion of <i>rsaA</i> C-terminal into plasmid containing <i>esp</i>; lanes 5-7: insertion of <i>esp</i> into plasmid containing <i>rsaA</i> C-terminal.  Smearing of bands is inconclusive and suggests that there may be no DNA (colonies are contaminants) or that freeze-thaw did not succeed in providing sufficient template DNA for PCR.
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Digested miniprep samples of overnight cultures of transformation product that may contain <i>esp</i> and <i>rsaA</i>. Lane 1: <i>esp</i> standard; lanes 2,3: <i>rsaA</i> from gel extraction inserted into pSB1C3 containing <i>esp</i> from plates spread with 20&mu;L of transformation cells; lanes 4,5: <i>rsaA</i> from gel extraction inserted into pSB1C3 containing <i>esp</i> from plates spread with 200&mu;L of transformation cells; lanes 6,7: <i>esp</i> from gel extraction inserted into pSB1C3 containing <i>rsaA</i> from plates spread with 20&mu;L of transformation cells; lanes 8,9: <i>esp</i> from gel extraction inserted into pSB1C3 containing <i>rsaA</i> from plates spread with 200&mu;L of transformation cells; lane 10: ladder.
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Lane 1: ladder; lane 2: digest of miniprep of transformation product of insert of <i>esp</i> from gel extraction into plasmid containing <i>rsaA</i>; lanes 3,4: ligations gel extracted <i>esp</i> and <i>rsaA</i> and plasmid containing the other gene; lane 5: ligation of gel extracted <i>esp</i> with <i>rsaA</i>. Lane 2 shows that ligation was unsuccessful. The rest of the lanes are inconclusive and a follow up gel with additional DNA is to be run.
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Lane 1: ladder; lane 2: ligation of <i>esp</i> and <i>rsaA</i> C-terminal fragments from gel extraction. No <i>rsaA</i> appears to be present, nor any ligation, only <i>esp</i>.
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Revision as of 22:59, 5 July 2011

Grinnell Menubar

Gel Pics

2011.06.05-2011.06.11

2011.06.12-2011.06.18

2011.06.19-2011.06.25

2011.06.26-2011.07.02

2011.07.03-2011.07.09