Team:Kyoto/Material

From 2011.igem.org

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==DNA==
 
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{| class="materials"
 
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!Name||Source or Reference
 
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==Primers==
==Primers==

Revision as of 08:00, 4 October 2011

Contents

Materials

Strain(Fruits Fly)

NameGenotype and/or Relevant FeaturesSource or Reference
D.melanogaster(fly)Oregon-R-CDGRC[http://kyotofly.kit.jp/cgi-bin/stocks/search_res_det.cgi?DB_NUM=1&DG_NUM=105669]

Strains(E.Coli)

NameGenotype and/or Relevant FeaturesSource or Reference
JM109endA, recA1, gyrA96,thi,hsdR17,(rκ-,mκ+),relA1, supE44,

λ-,Δ(lac-proAB),[F',traD36,proAB,laclqZΔM15]

Promega[http://www.promega.com/products/cloning-and-dna-markers/cloning-tools-and-competent-cells/bacterial-strains-and-competent-cells/bacterial-strain-jm109_de3_/]
NameGenotype and/or Relevant FeaturesSource or Reference
TOP10F-, mcrA, Δ(mrr-hsdRMS-mcrBC), φ80lacZΔM15, ΔlacX74, nupG, recA1, araD139, Δ(ara-leu)7697, galE15, galK16 rpsL(StrR), endA1, λ-Invitrogen
KRX[F′, traD36, ΔompP, proA+B+, lacIq, Δ(lacZ)M15] ΔompT, endA1, recA1, gyrA96 (Nalr), thi-1, hsdR17 (rk, mk+), e14– (McrA–), relA1, supE44, Δ(lac-proAB), Δ(rhaBAD)::T7 RNA polymerase.Laboratory of Gene Biodynamics, Graduate School of Biostudies, Kyoto University
(Promega [http://www.promega.com/catalog/catalogproducts.aspx?categoryname=productleaf_1698])
BL21(DE3)pLysSF–, ompT, hsdSB (rB, mB), dcm, gal, λ(DE3), pLysS, Cmr.Promega [http://www.promega.com/catalog/catalogproducts.aspx?categoryname=productleaf_1005]


Primers

DateNameSequence
07/21S Forward (Same as SRRz Forward)gGAATTCGCGGCCGCTTCTAGAGgcctggatttgttctatcagtaatcga
07/21S ReverseCTGCAGCGGCCGCTACTAGTAttattgatttctaccatcttctactccggc
07/21SRRz ReverseCTGCAGCGGCCGCTACTAGTAtgagttgcccatcgatatgggc
07/28ΔTMD1 Forwardcgcggcagatataatggcggt
07/28ΔTMD1 Reversegtcatgtttttctggcatcttcatgtct
08/19, 08/24Sam7 Forwardggttcattcgtgaccttctcgacttc
08/19, 08/24Sam7 Reverseaggcgataatggcgcacatc
08/20SRRz Forward RetrygGAATTCGCGGCCGCTTCTAGAGgagcaaatccccttattgggggtaagacatg
08/20SRRz Reverse RetryCTGCAGCGGCCGCTACTAGTAgcaactctatctgcactgctcattaatatacttctgg
08/23, 08/24Deletion of Upstream Forwardgagcaaatccccttattgggggtaagacatg
08/23, 08/24Deletion of Upstream Reversectctagaagcggccgcgaattcc
09/13, 09/22Add J23105 ForwardCAGTCCTAGGTACTATGCTAGCtactagtagcggccgctgc
09/13, 09/22Add J23105 ReverseAGCTAGCCGTAAActctagtatataaacgcagaaaggcccacc
10/10Deletion of GFP Forward (Primer1)ccaggcatcaaataaaacgaaaggctc
10/10Deletion of GFP-DT Forward (Primer2)tactagtagcggccgctgc
10/10Deletion of GFP, GFP-DT Reverse (Primer3)ctctagtattattgatttctaccatcttctactcc
- VF2 (<partinfo>G00100</partinfo>)tgccacctgacgtctaagaa
- VF (<partinfo>G00101</partinfo>)attaccgcctttgagtgagc