Team:Tec-Monterrey/projectmodeling

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  The first DNA construction our team used, was called “Construct C” this assembly was made in order to obtain data from our AraC-Pbad promoter. Our team worked with parts from British Columbia 09 team. Part BBa_K206000, pBAD,  is an <i>E.coli<i> promoter that is induced by L-arabinose. In the absence of arabinose, the repressor protein AraC, BBa_I13458, binds to the AraI1 operator site of pBAD and the upstream operator site AraO2, blocking transcription [1]. In the presence of arabinose, AraC binds to it and changes its conformation such that it interacts with the AraI1 and AraI2 operator sites, permitting transcription.
  The first DNA construction our team used, was called “Construct C” this assembly was made in order to obtain data from our AraC-Pbad promoter. Our team worked with parts from British Columbia 09 team. Part BBa_K206000, pBAD,  is an <i>E.coli<i> promoter that is induced by L-arabinose. In the absence of arabinose, the repressor protein AraC, BBa_I13458, binds to the AraI1 operator site of pBAD and the upstream operator site AraO2, blocking transcription [1]. In the presence of arabinose, AraC binds to it and changes its conformation such that it interacts with the AraI1 and AraI2 operator sites, permitting transcription.
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Revision as of 04:10, 29 September 2011

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