Team:Panama/Protocols/Wetlab

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18. [[Cell Culture]]
18. [[Cell Culture]]
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Revision as of 07:25, 28 September 2011

1. Agarose gel electrophoresis

2. Transformation

3. Colony PCR

4. Pgem Cloning

5. Preparing chemically competent cells

6. LB media recipe

7. Ampicillin stock

8.Chloramphenico stock

9. Mutagenesis PCR

10. Mutagenesis transformation

11. Miniprep Quiagen Kit

12. Miniprep "Dirty"

13. PCR purification

14. Glycerol Stock

15. PCR

16. Agar plates

17. Primer aliquots

18. Cell Culture

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