Team:Calgary/Notebook/Protocols/Process20
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Revision as of 22:48, 27 September 2011
cDNA Synthesis/Reverse Transcription Reaction
Reagents and Materials
- Overnight bacterial growth
- screw captubes
- glycerol
Protocol
- Pipet 0.5mL of 50% glycerol into 3 1.5 screw cap tubes
- Add 0.5mL of overnight culture to each tube
- Pipet up and down to gently mix
- Flash freeze in liquid N2 or dry ice/ethanol bath
- Place in -80 C freezer when frozen