Team:Minnesota/davis950-08162011135544-17
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{{Team:Minnesota/Top}} | {{Team:Minnesota/Top}} | ||
- | <br>== Digest of pcyA and ho1 == | + | <br> |
+ | == Digest of pcyA and ho1 == | ||
'''Project:''' Regulatory | '''Project:''' Regulatory | ||
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<nowiki>ho1 digest product should be treated with phosphatase to prevent religation to self. pcyA digest should be gel purified and extracted to remove backbone. These two products should be ligated together, transformed and a colony PCR should verify which colonies contain the full construct.</nowiki> | <nowiki>ho1 digest product should be treated with phosphatase to prevent religation to self. pcyA digest should be gel purified and extracted to remove backbone. These two products should be ligated together, transformed and a colony PCR should verify which colonies contain the full construct.</nowiki> | ||
+ | |||
{{Team:Minnesota/Bottom}} | {{Team:Minnesota/Bottom}} |
Latest revision as of 22:46, 27 September 2011
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