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Team:Glasgow/BiofilmResults
From 2011.igem.org
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<h2>Summary</h2> | <h2>Summary</h2> | ||
<p>- New Chassis</p> | <p>- New Chassis</p> | ||
| + | <p>-Transformable</p> | ||
<p>- Forms biofilms</p> | <p>- Forms biofilms</p> | ||
<p>- Non-pathogenic and compatible with majority of biobricks</p> | <p>- Non-pathogenic and compatible with majority of biobricks</p> | ||
<p>-No shuttle vector necessary</p> | <p>-No shuttle vector necessary</p> | ||
<p>-Time series shows that biofilm grows at similar speed to planktonic cells</p> | <p>-Time series shows that biofilm grows at similar speed to planktonic cells</p> | ||
| + | <p><b>Well suited for biofilm investigation, especially when intending to transform the biofilm</b></p> | ||
Revision as of 01:05, 22 September 2011
Results
The images below show a selection of stages of biofilm formation. Starting with Image 1 showing a lab strain of E.colithat has no fimbriae, and is not forming a biofilm.
Image 2 shows an EM of E.coli Nissle 1917 in the early stages of biofilm formation. The fimbriae that allow the cells to cling to each other are clearly visible.
Image 3 shows a Nissle biofilm in the later stages of formation, with the cells densely packed and the extracellular matrix that holds them together showing.
Image 1: 15,000x EM of E.coli for comparison. No fimbriae or EPS is visible. (courtesy of Rocky Mountain Laboratories) |
 Picture 4: 10,000x SEM image of Nissle showing the fimbriae |
 Image 3: SEM image of Nissle biofilm showing the extracellular matrix |
Image 1: 1000x EM of P. aeruginosa biofilm, showing its densely packed structure (courtesy of Dan Walker, University of Glasgow) |
Summary
- New Chassis
-Transformable
- Forms biofilms
- Non-pathogenic and compatible with majority of biobricks
-No shuttle vector necessary
-Time series shows that biofilm grows at similar speed to planktonic cells
Well suited for biofilm investigation, especially when intending to transform the biofilm
