Team:Glasgow/BiofilmResults

From 2011.igem.org

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<p>Biofilm formation was also confirmed by SEM pictures that showed the extracellular matrix, such as Picture 1 below.</p>
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<h1>Results</h1>
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<p>The images below show a selection of stages of biofilm formation. Starting with Image 1 showing a lab strain of <i>E.coli</i>that has no fimbriae, and is not forming a biofilm.</p>
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<p>Image 2 shows an EM of <i>E.coli</i> Nissle 1917 in the early stages of biofilm formation. The fimbriae that allow the cells to cling to each other are clearly visible.</p>
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<p>Image 3 shows a Nissle biofilm in the later stages of formation, with the cells densely packed and the extracellular matrix that holds them together showing.</p>
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<img src="http://farm7.static.flickr.com/6177/6166139079_a35d6a5930_m.jpg" width="230" height="180" /><p><font size="1" color="grey">Picture 1: SEM image of Nissle biofilm </br>showing the extracellular matrix</font></p>
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<img src="http://farm7.static.flickr.com/6171/6170367511_51e5363dbd_m.jpg" width="230" height="170"/>
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<p><font size="1" color="grey"> Image 1: 15,000x EM of E.coli for comparison. </br>No fimbriae or EPS is visible. (courtesy of Rocky Mountain Laboratories)</font></p>
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<img src="http://farm7.static.flickr.com/6156/6166741226_e4cfd217bd_m.jpg" /><p><font size="1" color="grey">Picture 4: 10,000x SEM image of Nissle </br>showing the fimbriae</font></p>
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<p><font size="1" color="grey"> Picture 2: 400x A gram-stained 16-hour </br><i>E.coli</i> Nissle biofilm </font></p>
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<img src="http://farm7.static.flickr.com/6161/6169966780_264a3b3147_m.jpg"/>
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<img src="http://farm7.static.flickr.com/6177/6166139079_a35d6a5930_m.jpg" width="230" height="180" /><p><font size="1" color="grey">Image 3: SEM image of Nissle biofilm </br>showing the extracellular matrix</font></p>
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<p><font size="1" color="grey"> Picture 3: 100x A gram stained 16-hour </br><i>E.coli</i> Nissle biofilm</font></p>
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<p><font size="1" color="grey">Image 1: 1000x EM of P. aeruginosa biofilm, showing its densely packed structure </br>(courtesy of Dan Walker, University of Glasgow)</font></p>
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<p>The images below may show why <i>E.coli</i> Nissle has the biofilm forming that <i>E.coli</i> lab strains lack. In Picture 4 the fimbriae of Nissle are clearly visible. Since the <i>E.coli</i> lab strain in Picture 5 does not have them, they may be the reason <i>E.coli</i> Nissle 1917 are so adept at clinging to each other.</p>
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<img src="http://farm7.static.flickr.com/6156/6166741226_e4cfd217bd_m.jpg" /><p><font size="1" color="grey">Picture 4: 10,000x SEM image of Nissle </br>showing the fimbriae</font></p>
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<p><font size="1" color="grey"> Image 2: 15,000x EM of E.coli for comparison. </br>No fimbriae or EPS is visible. (courtesy of Rocky Mountain Laboratories)</font></p>
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Revision as of 00:53, 22 September 2011

Results

The images below show a selection of stages of biofilm formation. Starting with Image 1 showing a lab strain of E.colithat has no fimbriae, and is not forming a biofilm.

Image 2 shows an EM of E.coli Nissle 1917 in the early stages of biofilm formation. The fimbriae that allow the cells to cling to each other are clearly visible.

Image 3 shows a Nissle biofilm in the later stages of formation, with the cells densely packed and the extracellular matrix that holds them together showing.

Image 1: 15,000x EM of E.coli for comparison.
No fimbriae or EPS is visible. (courtesy of Rocky Mountain Laboratories)

Picture 4: 10,000x SEM image of Nissle
showing the fimbriae

Image 3: SEM image of Nissle biofilm
showing the extracellular matrix

Image 1: 1000x EM of P. aeruginosa biofilm, showing its densely packed structure
(courtesy of Dan Walker, University of Glasgow)