Team:Bilkent UNAM Turkey/Experiment

From 2011.igem.org

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We repeat this step a lot because of failuire.I put right result<br>
We repeat this step a lot because of failuire.I put right result<br>
<img src="https://static.igem.org/mediawiki/2011/2/2f/C.reinhardtii_genler.png" align="center"></img>
<img src="https://static.igem.org/mediawiki/2011/2/2f/C.reinhardtii_genler.png" align="center"></img>
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<img src="https://static.igem.org/mediawiki/2011/9/9b/XhoI_and_BamHI_restriction_digestion_result.png" align="center"></img>
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<img src="https://static.igem.org/mediawiki/2011/c/c7/Confirmation_of_Ligation_Products_with_XhoI_and_BamHI_restriction_digestion.png" align="center"></img>
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<br>We used standard <a href="http://tools.invitrogen.com/content/sfs/manuals/purelink_quick_gel_extraction_kit_man.pdf">gel purification kit</a>
<br>We used standard <a href="http://tools.invitrogen.com/content/sfs/manuals/purelink_quick_gel_extraction_kit_man.pdf">gel purification kit</a>
Our ligation style is really similar to iGEM's one.<br>
Our ligation style is really similar to iGEM's one.<br>

Revision as of 21:33, 21 September 2011





We ordered our genes. We optimized codon usage because of gene taken from E. cloacae.

Codon Usage Then we did restriction digestion and gel electrophoresis.
We repeat this step a lot because of failuire.I put right result

We used standard gel purification kit Our ligation style is really similar to iGEM's one.
Our one;

  • insert volume(µl):X
  • Cut Vector Volume(µl):X
  • DEPC-water(µl):8,5-2X
  • T4 DNA ligase(µl):0,5
  • Ligation Buffer(µl):1
  • Total Volume(µl):10

    • We transformed our plasmid into E.coli with a protocol.
    We used invitrogen purification kit to get our plasmid. We sent genes to sequencing and sequencing data should be available on their link.
    There is a problem with poping up if you see this note.

    Retrieved from "http://2011.igem.org/Team:Bilkent_UNAM_Turkey/Experiment"