Team:Bilkent UNAM Turkey/Experiment

(Difference between revisions)

Revision as of 20:42, 21 September 2011

We ordered our genes. We optimized codon usage because of gene taken from E. cloacae.

Codon Usage Then we did restriction digestion and gel electrophoresis.
We repeat this step a lot because of failuire.I put right result

We used standard gel purification kit Our ligation style is really similar to iGEM's one.
Our one;

insert volume(µl):X

Cut Vector Volume(µl):X

DEPC-water(µl):8,5-2X

T4 DNA ligase(µl):0,5

Ligation Buffer(µl):1

Total Volume(µl):10

We transform our plasmid into E.coli with a protocol.
We used invitrogen purification kit to get our plasmid.

@@ Line 15: / Line 15: @@
 <li>Total Volume(µl):10</li>
 <br>We transform our plasmid into E.coli with a <a href="http://www.med.nyu.edu/medicine/labs/blaserlab/Protocols/E-coli_competent_cells_protocol_&_transformation.pdf">protocol.</a><br>
-We used invitrogen <a href="http://tools.invitrogen.com/content/sfs/manuals/purelink_pro96_plasmid_kit_man.pdf">purification kit.</a>
+We used invitrogen <a href="http://tools.invitrogen.com/content/sfs/manuals/purelink_pro96_plasmid_kit_man.pdf">purification kit </a>to get our plasmid.
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