Team:Glasgow/LOVresults

From 2011.igem.org

(Difference between revisions)
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<br>4)Make LOV2 fluoresce under UV light
<br>4)Make LOV2 fluoresce under UV light
</br></br>
</br></br>
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<br>5)Transform LOV2 into sumbission vector
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<br>5)Ligate LOV2 into sumbission vector
</br></br>
</br></br>
<br>6)Submit LOV 2
<br>6)Submit LOV 2
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</br></br>
</br></br>
<h3>Aims</h3>
<h3>Aims</h3>
 +
 +
<br>1) Design iLOV construct to be synthesised
 +
</br></br>
 +
<br>2) Get iLOV synthesised
 +
</br></br>
 +
<br>3)Transform iLOV cells and make fluoresce
 +
</br></br>
 +
<br>4)Ligate iLOV into sybmission vector
 +
</br></br>
 +
<br>5)Submit iLOV
 +
</br></br>

Revision as of 14:30, 21 September 2011

LOV2 Results







Aims


1)Perform restriction digest to ensure we have LOV2 domain


2) Design site directed mutagenesis primers to get rid of illegal pst1 site


3)To perform site directed mutagenesis on LOV2


4)Make LOV2 fluoresce under UV light


5)Ligate LOV2 into sumbission vector


6)Submit LOV 2

{{Team:Glasgow/Header}}

iLOV Results







Aims


1) Design iLOV construct to be synthesised


2) Get iLOV synthesised


3)Transform iLOV cells and make fluoresce


4)Ligate iLOV into sybmission vector


5)Submit iLOV

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