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Team:Glasgow/LOVresults
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| - | 1)Perform restriction digest to ensure we have LOV2 domain | + | <br>1)Perform restriction digest to ensure we have LOV2 domain |
</br></br> | </br></br> | ||
| - | 2) Design site directed mutagenesis primers to get rid of illegal pst1 site | + | <br>2) Design site directed mutagenesis primers to get rid of illegal pst1 site |
</br></br> | </br></br> | ||
| - | 3)To perform site directed mutagenesis on LOV2 | + | <br>3)To perform site directed mutagenesis on LOV2 |
</br></br> | </br></br> | ||
| - | 4)Make LOV2 fluoresce under UV light | + | <br>4)Make LOV2 fluoresce under UV light |
</br></br> | </br></br> | ||
| - | 5)Transform LOV2 into sumbission vector | + | <br>5)Transform LOV2 into sumbission vector |
</br></br> | </br></br> | ||
6)Submit LOV 2 | 6)Submit LOV 2 | ||
Revision as of 14:23, 21 September 2011
LOV2 Results
Aims
1)Perform restriction digest to ensure we have LOV2 domain
2) Design site directed mutagenesis primers to get rid of illegal pst1 site
3)To perform site directed mutagenesis on LOV2
4)Make LOV2 fluoresce under UV light
5)Transform LOV2 into sumbission vector 6)Submit LOV 2
