Team:Freiburg/Notebook/6 September

From 2011.igem.org

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Trafo of ligated parts of the 2A-assembly.
Trafo of ligated parts of the 2A-assembly.
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===Sequence analysis==
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sequence analysis of our lovtap-Notgate was neagtive:-( So we´ll try the gibson assembly.
==<span style="color:red;">red light receptor</span>==
==<span style="color:red;">red light receptor</span>==

Revision as of 11:06, 6 September 2011


This is the wiki page
of the Freiburger student
team competing for iGEM 2011.
Thank you for your interest!

Contents

Commons

Miniprep

Investigators: Sandra

Yesterday LB was inoculated with strains (S14, S39-S44 etc.). Miniprep of strains.


Digestion: 2A-assembly

Investigators: Sandra

Digestion of:

  • S14 (GFP)

with XbaI and PstI

  • S39 (PR1)
  • S40 (PR2)
  • S41 (PR3)
  • S42 (PR4)
  • S43 (PR5)
  • S44 (PR6)

with SpeI and PstI

incubation for 6 hours at 37°C.

green light receptor

NAME OF YOUR EXPERIMENT

Investigators:NAME


blue light receptor

Trafo

Investigators: Sandra

Trafo of ligated parts of the 2A-assembly.

=Sequence analysis

sequence analysis of our lovtap-Notgate was neagtive:-( So we´ll try the gibson assembly.

red light receptor

NAME OF YOUR EXPERIMENT

Investigators:NAME



Lysis cassette

NAME OF YOUR EXPERIMENT

Investigators:NAME


Precipitator

NAME OF YOUR EXPERIMENT

Investigators: NAME

                       

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