Team:Kyoto/Luminescence/Note

From 2011.igem.org

(Difference between revisions)
Line 2: Line 2:
== Aug 4th, 2011 ==
== Aug 4th, 2011 ==
-
# We get drosophila melanogaster(oregon-rs) in 3 tubes from [http://gcoe.biol.sci.kyoto-u.ac.jp/gcoe/eng/member/2008/07/fuse_naoyuki.php Mr. Fuse].
+
* We get drosophila melanogaster(oregon-rs) in 3 tubes from [http://gcoe.biol.sci.kyoto-u.ac.jp/gcoe/eng/member/2008/07/fuse_naoyuki.php Mr. Fuse].
-
# We make [http://www.biol.se.tmu.ac.jp/fly/www/standard-medium.html standard feed of Tokyo Metropolitan Univ. for drosophila].
+
* We make [http://www.biol.se.tmu.ac.jp/fly/www/standard-medium.html standard feed of Tokyo Metropolitan Univ. for drosophila].
-
:#Materials
+
::{| class="wikitable"
-
::* water 500mL
+
|+ [http://www.biol.se.tmu.ac.jp/fly/www/standard-medium.html Standard feed of Tokyo Metropolitan Univ. for drosophila]
-
::* dry yeast 20g
+
! width="300" |Materials
-
::* corn flour 45g
+
! width="300" | Making
-
::* glucose 50g
+
|-
-
::* agalose 3.5g
+
|
-
::* propionic acid 1.5mL
+
* water 500mL
-
:#Making
+
* dry yeast 20g
-
::# Stir dry yeast and agalose with about two-thirds of water. Then, autoclave it.
+
* corn flour 45g
 +
* glucose 50g
 +
* agalose 3.5g
 +
* propionic acid 1.5mL
 +
|
 +
# Stir dry yeast and agalose with about two-thirds of water. Then, autoclave it.
 +
# Stir corn flour and glucose with the remaining water.
 +
# Stir 1 and 2, then autoclave it again.
 +
# after autoclave, add propionic acid into it.■
 +
|}
 +
* We dispense the standard-medium into 3 bottles.

Revision as of 11:11, 30 August 2011

This page is the note of Luminescence Group.

Aug 4th, 2011

  • We get drosophila melanogaster(oregon-rs) in 3 tubes from [http://gcoe.biol.sci.kyoto-u.ac.jp/gcoe/eng/member/2008/07/fuse_naoyuki.php Mr. Fuse].
  • We make [http://www.biol.se.tmu.ac.jp/fly/www/standard-medium.html standard feed of Tokyo Metropolitan Univ. for drosophila].
[http://www.biol.se.tmu.ac.jp/fly/www/standard-medium.html Standard feed of Tokyo Metropolitan Univ. for drosophila]
Materials Making
  • water 500mL
  • dry yeast 20g
  • corn flour 45g
  • glucose 50g
  • agalose 3.5g
  • propionic acid 1.5mL
  1. Stir dry yeast and agalose with about two-thirds of water. Then, autoclave it.
  2. Stir corn flour and glucose with the remaining water.
  3. Stir 1 and 2, then autoclave it again.
  4. after autoclave, add propionic acid into it.■
  • We dispense the standard-medium into 3 bottles.