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Team:British Columbia/Notebook/4 July 2011
From 2011.igem.org
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*Amplified 5 plasmids received from Greece (IDI1, HMG2, p300, Cin, Sab). NOTE: did not properly centrifuge during DNA precipitation stage. Will validate plasmids in yeast transformations to make sure plasmids are in working order. | *Amplified 5 plasmids received from Greece (IDI1, HMG2, p300, Cin, Sab). NOTE: did not properly centrifuge during DNA precipitation stage. Will validate plasmids in yeast transformations to make sure plasmids are in working order. | ||
*Awaiting materials from France (erg20-2). | *Awaiting materials from France (erg20-2). | ||
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| + | ====1,8-Cineole==== | ||
| + | *Ran a gel to test restriction digest and variant SDM-PCR product. | ||
Revision as of 20:33, 4 July 2011
Contents |
July 04 2011
Week of Jun27 - Jul04
IDI1, HMG2, erg20-2
- Validated competent cells by transforming with pet100 vector containing Cineole synthase. They are in working order.
- Amplified 5 plasmids received from Greece (IDI1, HMG2, p300, Cin, Sab). NOTE: did not properly centrifuge during DNA precipitation stage. Will validate plasmids in yeast transformations to make sure plasmids are in working order.
- Awaiting materials from France (erg20-2).
1,8-Cineole
- Ran a gel to test restriction digest and variant SDM-PCR product.
